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Dataset: Histone exchange assay in loss of DOT1

External ID
GSE106450
Reference
Lee S, et al. (2018)
Channels
1
Conditions
40
Description
Strain background is Saccharomyces cerevisiae wzy42 histone mutant strain with pWZ403 Myc-HHT2-HHF2 plasmid and pGAL1/10 FLAG-HHT1-HHF1 integrated gene. Cells were grown in YP+2% raffinose until mid-log (OD600=0.5) and arrested with alpha factor. When most of Cells were in G1 phase, added galactose (final conc. 2%) and harvested cells after 1 hour. Fixed cells were harvested by centrifugation at 4 Celcius degrees and the cell pellets were washed by 15mL of pH7.5 TBS buffer per a conical tube. ChIP cells were disrupted by glass bead vortexing in ChIP lysis buffer and sonicated by Sonic Dismembrator Model 500, Fisher Scientific. Sonicated soluble lysate were immunoprecipiated by FLAG antibody (F1804; Sigma).
Categories
histone modification, chromatin organization

Conditions

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ConditionExternal ID
ATAC_dot1DGSM2837737
ATAC_sas2DGSM2837736
ATAC_set2DGSM2837738
ATAC_WTGSM2837739
FLAG_dot1DGSM2837728
FLAG_sas2DGSM2884465
FLAG_WTGSM2837727
H3_Dot1101-140DGSM2837725
H3_dot1DGSM2837722
H3_dot1Dset2DGSM2837724
Showing 1 to 10 of 40 entries