Complex: CHL1-CTF4 complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
- Curated by
- IntAct
- ComplexAc
- CPX-26727
- Systematic Name
- CHL1:3xCTF4
- SGD ID
- SGD:S000381795
- Aliases
- Replisome accessory complex
- Description
- Functions as a replisome-organizing module that coordinates DNA replication fork progression with sister chromatid cohesion establishment during S phase. CTF4 acts as a replisomal interaction hub, recruiting the CHL1 helicase to the CMG helicase (CPX-297) via a conserved binding motif shared with GINS (CPX-1641) and DNA polymerase-alpha. This coupling links DNA unwinding to lagging-strand priming, promoting efficient replication, particularly under conditions of nucleotide depletion.
Complex Diagram
This diagram displays the protein subunits (blue) of the complex and how they interact with each other. Interactions with other relevant participants such as small molecules (purple), sub-complexes (yellow), and other subunits (red) are also shown. If a participant appears in the diagram with no lines connected to it, then its specific interactions with other participants are currently unknown.
Subunits
This table lists all participants of the complex (proteins, small molecules, nucleic acids, etc.) and their respective stoichiometry. A brief description is also provided for each participant in the table.
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| Subunit | Description | Stoichiometry |
|---|
Gene Ontology
Gene Ontology (GO) terms that describe the function of a complex, the biological process in which it participates, or its cellular location. Click on a term for its definition and other objects annotated to that term.
- Molecular Function
-
- enables DNA helicase activity (IDA)
- enables DNA replication origin binding (BSR)
- Biological Process
-
- involved in mitotic DNA replication initiation (BSR)
- involved in mitotic sister chromatid cohesion (BSR)
- involved in regulation of DNA-templated DNA replication initiation (BSR)
- Cellular Component
-
- located in replication fork (IPI)
Shared Annotations
This diagram displays Gene Ontology terms (green) and subunits (blue) that are shared between the given macromolecular complex (black) and other yeast complexes (yellow). The shared GO terms or subunits can be visualized separately using the radio button at the bottom of the graph. Note: No diagram is shown ("No shared annotations") if there are less than 2 shared annotations (either GO terms or subunits of other complexes) between this complex and any other complexes.
References
List of references used specifically to curate the information on this page.
- Wu Y, et al. (2017) Targeting the Genome-Stability Hub Ctf4 by Stapled-Peptide Design. Angew Chem Int Ed Engl 56(42):12866-12872 PMID: 28815832
- Samora CP, et al. (2016) Ctf4 Links DNA Replication with Sister Chromatid Cohesion Establishment by Recruiting the Chl1 Helicase to the Replisome. Mol Cell 63(3):371-84 PMID: 27397686
- Simon AC, et al. (2014) A Ctf4 trimer couples the CMG helicase to DNA polymerase α in the eukaryotic replisome. Nature 510(7504):293-297 PMID: 24805245
- Gambus A, et al. (2009) A key role for Ctf4 in coupling the MCM2-7 helicase to DNA polymerase alpha within the eukaryotic replisome. EMBO J 28(19):2992-3004 PMID: 19661920
Resources
IntAct | ModelArchive | PDB:4C8H | PDB:4C8S ... Show all Show fewer