Restriction Fragments for SUP6/tY(GUA)F2



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The currently selected gene/sequence is :

SUP6/tY(GUA)F2 on chromosome VI from coordinates 210707 to 210619.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.21445.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 8 13 + TsoI TARCCA 24 22 . . 9 12 + CviJI RGCY 10 10 . . 21 24 + MseI TTAA 21 23 . . 26 29 + Hin6I GCGC 26 28 . . 26 29 + GlaI GCGC 27 27 . . 26 29 + HhaI GCGC 28 26 . . 33 37 + Tsp4CI* ACNGT 35 34 . . 38 41 + TspEI AATT 37 41 . . 55 60 + Hpy178III* TCNNGA 56 58 . . 56 61 + SmlI CTYRAG 56 60 . . 56 61 + Bce83I* CTTGAG 77 75 . . 61 64 + DpnI GATC 62 62 . . 61 64 + BstKTI GATC 63 61 . . 61 64 + MboI GATC 60 64 . . 71 74 + TaqI TCGA 71 73 . . 73 77 + HinfI GANTC 73 76 . . 73 77 - PleI GAGTC 67 68 . . 73 77 - MlyI GAGTC 67 67 . . 80 85 + SecI* CCNNGG 80 84 . . 81 86 + SmaI CCCGGG 83 83 . . 81 86 + Cfr9I CCCGGG 81 85 . . 81 85 + BssKI CCNGG 80 85 . . 81 86 + SecI* CCNNGG 81 85 . . 81 86 + BmeT110I CYCGRG 82 84 . . 81 85 + ScrFI CCNGG 82 83 . . 81 85 + CauII* CCSGG 82 83 . . 81 86 + AvaI CYCGRG 81 85 . . 82 86 + BssKI CCNGG 81 86 . . 82 85 + HpaII CCGG 82 84 . . 82 86 + ScrFI CCNGG 83 84 . . 82 86 + CauII* CCSGG 83 84 . . #--------------------------------------- # # # AvaI: # [CYCGRG] # 8 81 # # Bce83I*: # [CTTGAG] # 12 77 # # BmeT110I: # [CYCGRG] # 7 82 # # BssKI: # [CCNGG] # 1 8 80 # # BstKTI: # [GATC] # 26 63 # # CauII*: # [CCSGG] # 1 6 82 # # Cfr9I: # [CCCGGG] # 8 81 # # CviJI: # [RGCY] # 10 79 # # DpnI: # [GATC] # 27 62 # # GlaI: # [GCGC] # 27 62 # # HhaI: # [GCGC] # 28 61 # # Hin6I: # [GCGC] # 26 63 # # HinfI: # [GANTC] # 16 73 # # HpaII: # [CCGG] # 7 82 # # Hpy178III*: # [TCNNGA] # 33 56 # # MboI: # [GATC] # 29 60 # # MlyI: # [GAGTC] # 22 67 # # MseI: # [TTAA] # 21 68 # # PleI: # [GAGTC] # 22 67 # # ScrFI: # [CCNGG] # 1 6 82 # # SecI*: # [CCNNGG] # 1 8 80 # # SmaI: # [CCCGGG] # 6 83 # # SmlI: # [CTYRAG] # 33 56 # # TaqI: # [TCGA] # 18 71 # # TsoI: # [TARCCA] # 24 65 # # Tsp4CI*: # [ACNGT] # 35 54 # # TspEI: # [AATT] # 37 52 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 89 # Reported_sequences: 1 # Reported_hitcount: 31 #---------------------------------------