Restriction Fragments for tS(AGA)D1



Send questions or suggestions to SGD

BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

tS(AGA)D1 on chromosome IV from coordinates 437772 to 437853.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.14426.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 8 13 + CfrI YGGCCR 8 12 . . 9 12 + CviJI RGCY 10 10 . . 9 12 + HaeIII GGCC 10 10 . . 10 15 + NmeAIII GCCGAG 36 34 . . 19 22 + MseI TTAA 19 21 . . 16 21 - TsoI TARCCA 6 4 . . 30 40 + Hin4I GAYNNNNNVTC 21 16 53 48 30 40 - Hin4I GAYNNNNNVTC 53 48 21 16 46 49 + CviJI RGCY 47 47 . . 47 57 + MwoI GCNNNNNNNGC 53 50 . . 52 57 + Cac8I GCNNGC 54 54 . . 53 57 + FauI CCCGC 61 63 . . 54 57 + AciI CCGC 54 56 . . 55 58 + FnuDII* CGCG 56 56 . . 56 59 + Hin6I GCGC 56 58 . . 56 59 + GlaI GCGC 57 57 . . 56 59 + HhaI GCGC 58 56 . . 60 63 + SetI ASST 63 59 . . 58 63 - BspMI ACCTGC 49 53 . . 64 67 + TaqI TCGA 64 66 . . 66 70 + PleI GAGTC 74 75 . . 66 70 + MlyI GAGTC 75 75 . . 66 70 + HinfI GANTC 66 69 . . 71 76 + PstI CTGCAG 75 71 . . 71 76 + SfeI* CTRYAG 71 75 . . 72 75 + CviRI* TGCA 73 73 . . #--------------------------------------- # # # AciI: # [CCGC] # 28 54 # # BspMI: # [ACCTGC] # 33 49 # # Cac8I: # [GCNNGC] # 28 54 # # CfrI: # [YGGCCR] # 8 74 # # CviJI: # [RGCY] # 10 35 37 # # CviRI*: # [TGCA] # 9 73 # # FauI: # [CCCGC] # 21 61 # # FnuDII*: # [CGCG] # 26 56 # # GlaI: # [GCGC] # 25 57 # # HaeIII: # [GGCC] # 10 72 # # HhaI: # [GCGC] # 24 58 # # Hin4I: # [GAYNNNNNVTC] # 0 0 21 29 32 # # Hin6I: # [GCGC] # 26 56 # # HinfI: # [GANTC] # 16 66 # # MlyI: # [GAGTC] # 7 75 # # MseI: # [TTAA] # 19 63 # # MwoI: # [GCNNNNNNNGC] # 29 53 # # NmeAIII: # [GCCGAG] # 36 46 # # PleI: # [GAGTC] # 8 74 # # PstI: # [CTGCAG] # 7 75 # # SetI: # [ASST] # 19 63 # # SfeI*: # [CTRYAG] # 11 71 # # TaqI: # [TCGA] # 18 64 # # TsoI: # [TARCCA] # 6 76 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 82 # Reported_sequences: 1 # Reported_hitcount: 26 #---------------------------------------