Restriction Fragments for tR(UCU)J2



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The currently selected gene/sequence is :

tR(UCU)J2 on chromosome X from coordinates 374577 to 374506.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.12249.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 1 11 + MwoI GCNNNNNNNGC 7 4 . . 1 6 + Cac8I GCNNGC 3 3 . . 4 7 + FnuDII* CGCG 5 5 . . 21 26 + MluI ACGCGT 21 25 . . 21 26 + AflIII ACRYGT 21 25 . . 22 25 + FnuDII* CGCG 23 23 . . 26 30 + Hpy188I TCNGA 28 27 . . 23 27 - HgaI GACGC 12 17 . . 29 39 + Hin4I GAYNNNNNVTC 20 15 52 47 38 42 + Hpy188I TCNGA 40 39 . . 29 39 - Hin4I GAYNNNNNVTC 52 47 20 15 41 45 + MboII GAAGA 53 52 . . 54 57 + TaqI TCGA 54 56 . . 61 65 + BccI CCATC 69 70 . . 64 69 + Hpy178III* TCNNGA 65 67 . . #--------------------------------------- # # # AflIII: # [ACRYGT] # 21 51 # # BccI: # [CCATC] # 3 69 # # Cac8I: # [GCNNGC] # 3 69 # # FnuDII*: # [CGCG] # 5 18 49 # # HgaI: # [GACGC] # 12 60 # # Hin4I: # [GAYNNNNNVTC] # 0 0 20 20 32 # # Hpy178III*: # [TCNNGA] # 7 65 # # Hpy188I: # [TCNGA] # 12 28 32 # # MboII: # [GAAGA] # 19 53 # # MluI: # [ACGCGT] # 21 51 # # MwoI: # [GCNNNNNNNGC] # 7 65 # # TaqI: # [TCGA] # 18 54 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 72 # Reported_sequences: 1 # Reported_hitcount: 15 #---------------------------------------