Restriction Fragments for tN(GUU)C



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

tN(GUU)C on chromosome III from coordinates 127716 to 127789.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.5601.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 1 5 + HinfI GANTC 1 4 . . 5 19 + XcmI CCANNNNNNNNNTGG 12 11 . . 5 10 + DsaI* CCRYGG 5 9 . . 5 10 + StyI CCWWGG 5 9 . . 5 10 + NcoI CCATGG 5 9 . . 5 10 + SecI* CCNNGG 5 9 . . 6 9 + FatI CATG 5 9 . . 6 9 + NlaIII CATG 9 5 . . 6 9 + CviAII CATG 6 8 . . 8 13 + BalI TGGCCA 10 10 . . 8 13 + CfrI YGGCCR 8 12 . . 9 12 + CviJI RGCY 10 10 . . 9 12 + HaeIII GGCC 10 10 . . 20 23 + MseI TTAA 20 22 . . 17 22 - TsoI TARCCA 7 5 . . 25 30 + Cac8I GCNNGC 27 27 . . 31 41 + Hin4I GAYNNNNNVTC 22 17 54 49 32 36 + Tsp4CI* ACNGT 34 33 . . 36 39 + MseI TTAA 36 38 . . 31 41 - Hin4I GAYNNNNNVTC 54 49 22 17 46 49 + DpnI GATC 47 47 . . 46 49 + BstKTI GATC 48 46 . . 46 49 + MboI GATC 45 49 . . 48 53 + Hpy178III* TCNNGA 49 51 . . 60 70 + BsiYI* CCNNNNNNNGG 66 63 . . 61 64 + MnlI CCTC 71 70 . . 61 71 + BsiYI* CCNNNNNNNGG 67 64 . . 64 70 + TspRI CASTGNN 70 61 . . 65 69 + BsrI ACTGG 70 68 . . #--------------------------------------- # # # BalI: # [TGGCCA] # 10 64 # # BsiYI*: # [CCNNNNNNNGG] # 1 7 66 # # BsrI: # [ACTGG] # 4 70 # # BstKTI: # [GATC] # 26 48 # # Cac8I: # [GCNNGC] # 27 47 # # CfrI: # [YGGCCR] # 8 66 # # CviAII: # [CATG] # 6 68 # # CviJI: # [RGCY] # 10 64 # # DpnI: # [GATC] # 27 47 # # DsaI*: # [CCRYGG] # 5 69 # # FatI: # [CATG] # 5 69 # # HaeIII: # [GGCC] # 10 64 # # Hin4I: # [GAYNNNNNVTC] # 0 0 20 22 32 # # HinfI: # [GANTC] # 1 73 # # Hpy178III*: # [TCNNGA] # 25 49 # # MboI: # [GATC] # 29 45 # # MnlI: # [CCTC] # 3 71 # # MseI: # [TTAA] # 16 20 38 # # NcoI: # [CCATGG] # 5 69 # # NlaIII: # [CATG] # 9 65 # # SecI*: # [CCNNGG] # 5 69 # # StyI: # [CCWWGG] # 5 69 # # TsoI: # [TARCCA] # 7 67 # # Tsp4CI*: # [ACNGT] # 34 40 # # TspRI: # [CASTGNN] # 4 70 # # XcmI: # [CCANNNNNNNNNTGG] # 12 62 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 74 # Reported_sequences: 1 # Reported_hitcount: 29 #---------------------------------------