Restriction Fragments for SUP56/tL(CAA)A



Send questions or suggestions to SGD

BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

SUP56/tL(CAA)A on chromosome I from coordinates 181141 to 181254.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.19032.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 8 13 + CfrI YGGCCR 8 12 . . 9 12 + CviJI RGCY 10 10 . . 9 12 + HaeIII GGCC 10 10 . . 10 15 + NmeAIII GCCGAG 36 34 . . 15 18 - AciI CCGC 15 17 . . 13 18 - BsrBI CCGCTC 15 15 . . 20 24 + DdeI CTNAG 20 23 . . 24 29 + NarI GGCGCC 25 27 . . 24 29 + KasI GGCGCC 24 28 . . 24 29 + DinI GGCGCC 26 26 . . 24 29 + HaeII RGCGCY 28 24 . . 24 29 + NlaIV GGNNCC 26 26 . . 24 29 + AcyI GRCGYC 25 27 . . 24 29 + HgiCI* GGYRCC 24 28 . . 25 28 + Hin6I GCGC 25 27 . . 25 28 + GlaI GCGC 26 26 . . 25 28 + HhaI GCGC 27 25 . . 31 35 + TfiI GAWTC 31 34 . . 31 35 + HinfI GANTC 31 34 . . 34 39 + Hpy178III* TCNNGA 35 37 . . 44 49 + Hpy178III* TCNNGA 45 47 . . 50 53 + AciI CCGC 50 52 . . 52 57 + BtsI GCAGTG 59 57 . . 53 59 + TspRI CASTGNN 59 50 . . 55 60 + Hpy166II GTNNAC 57 57 . . 59 63 + Tsp4CI* ACNGT 61 60 . . 70 80 + BaeI ACNNNNGTAYC 59 54 92 87 71 75 + DdeI CTNAG 71 74 . . 71 75 + BspCNI CTCAG 84 82 . . 71 75 + BseMII CTCAG 85 83 . . 74 77 + SetI ASST 77 73 . . 87 90 + CviRI* TGCA 88 88 . . 85 89 - SfaNI GCATC 75 79 . . 95 100 + AsuII TTCGAA 96 98 . . 96 99 + TaqI TCGA 96 98 . . 98 102 + TfiI GAWTC 98 101 . . 98 102 + HinfI GANTC 98 101 . . 104 108 + DdeI CTNAG 104 107 . . #--------------------------------------- # # # AciI: # [CCGC] # 15 35 64 # # AcyI: # [GRCGYC] # 25 89 # # AsuII: # [TTCGAA] # 18 96 # # BaeI: # [ACNNNNGTAYC] # 22 33 59 # # BseMII: # [CTCAG] # 29 85 # # BspCNI: # [CTCAG] # 30 84 # # BsrBI: # [CCGCTC] # 15 99 # # BtsI: # [GCAGTG] # 55 59 # # CfrI: # [YGGCCR] # 8 106 # # CviJI: # [RGCY] # 10 104 # # CviRI*: # [TGCA] # 26 88 # # DdeI: # [CTNAG] # 10 20 33 51 # # DinI: # [GGCGCC] # 26 88 # # GlaI: # [GCGC] # 26 88 # # HaeII: # [RGCGCY] # 28 86 # # HaeIII: # [GGCC] # 10 104 # # HgiCI*: # [GGYRCC] # 24 90 # # HhaI: # [GCGC] # 27 87 # # Hin6I: # [GCGC] # 25 89 # # HinfI: # [GANTC] # 16 31 67 # # Hpy166II: # [GTNNAC] # 57 57 # # Hpy178III*: # [TCNNGA] # 10 35 69 # # KasI: # [GGCGCC] # 24 90 # # NarI: # [GGCGCC] # 25 89 # # NlaIV: # [GGNNCC] # 26 88 # # NmeAIII: # [GCCGAG] # 36 78 # # SetI: # [ASST] # 37 77 # # SfaNI: # [GCATC] # 39 75 # # TaqI: # [TCGA] # 18 96 # # TfiI: # [GAWTC] # 16 31 67 # # Tsp4CI*: # [ACNGT] # 53 61 # # TspRI: # [CASTGNN] # 55 59 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 114 # Reported_sequences: 1 # Reported_hitcount: 38 #---------------------------------------