Restriction Fragments for tD(GUC)L1



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

tD(GUC)L1 on chromosome XII from coordinates 427132 to 427203.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.6259.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 12 15 + MseI TTAA 12 14 . . 19 23 + Hpy188I TCNGA 21 20 . . 27 32 + HaeII RGCGCY 31 27 . . 28 31 + Hin6I GCGC 28 30 . . 28 31 + GlaI GCGC 29 29 . . 28 38 + MwoI GCNNNNNNNGC 34 31 . . 28 31 + HhaI GCGC 30 28 . . 36 39 + FnuDII* CGCG 37 37 . . 37 42 + Cac8I GCNNGC 39 39 . . 42 52 + BsiYI* CCNNNNNNNGG 48 45 . . 45 48 + DpnI GATC 46 46 . . 45 48 + BstKTI GATC 47 45 . . 45 48 + MboI GATC 44 48 . . 56 59 + TspEI AATT 55 59 . . 60 70 + BsiYI* CCNNNNNNNGG 66 63 . . 63 67 + Hpy99I CGWCG 67 62 . . 66 69 + FnuDII* CGCG 67 67 . . 67 70 - AciI CCGC 67 69 . . #--------------------------------------- # # # AciI: # [CCGC] # 5 67 # # BsiYI*: # [CCNNNNNNNGG] # 6 18 48 # # BstKTI: # [GATC] # 25 47 # # Cac8I: # [GCNNGC] # 33 39 # # DpnI: # [GATC] # 26 46 # # FnuDII*: # [CGCG] # 5 30 37 # # GlaI: # [GCGC] # 29 43 # # HaeII: # [RGCGCY] # 31 41 # # HhaI: # [GCGC] # 30 42 # # Hin6I: # [GCGC] # 28 44 # # Hpy188I: # [TCNGA] # 21 51 # # Hpy99I: # [CGWCG] # 5 67 # # MboI: # [GATC] # 28 44 # # MseI: # [TTAA] # 12 60 # # MwoI: # [GCNNNNNNNGC] # 34 38 # # TspEI: # [AATT] # 17 55 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 72 # Reported_sequences: 1 # Reported_hitcount: 18 #---------------------------------------