Restriction Fragments for tC(GCA)Q



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

tC(GCA)Q on mitochondria from coordinates 64415 to 64490.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.13449.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 1 11 - BsaXI ACNNNNNCTCC 23 20 . . 13 16 + MseI TTAA 13 15 . . 16 19 + SetI ASST 19 15 . . 19 22 + MseI TTAA 19 21 . . 30 40 + Hin4I GAYNNNNNVTC 21 16 53 48 33 36 + CviRI* TGCA 34 34 . . 30 40 - Hin4I GAYNNNNNVTC 53 48 21 16 47 50 + MseI TTAA 47 49 . . 55 58 + TaqI TCGA 55 57 . . 57 61 + TfiI GAWTC 57 60 . . 57 61 + HinfI GANTC 57 60 . . 61 66 + Ksp632I* CTCTTC 67 70 . . 62 66 - MboII GAAGA 54 53 . . 64 68 - TspDTI ATGAA 54 52 . . 72 75 + MseI TTAA 72 74 . . #--------------------------------------- # # # BsaXI: # [ACNNNNNCTCC] # 23 53 # # CviRI*: # [TGCA] # 34 42 # # Hin4I: # [GAYNNNNNVTC] # 0 0 21 23 32 # # HinfI: # [GANTC] # 19 57 # # Ksp632I*: # [CTCTTC] # 9 67 # # MboII: # [GAAGA] # 22 54 # # MseI: # [TTAA] # 4 6 13 25 28 # # SetI: # [ASST] # 19 57 # # TaqI: # [TCGA] # 21 55 # # TfiI: # [GAWTC] # 19 57 # # TspDTI: # [ATGAA] # 22 54 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 76 # Reported_sequences: 1 # Reported_hitcount: 15 #---------------------------------------