Restriction Fragments for tA(AGC)F



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The currently selected gene/sequence is :

tA(AGC)F on chromosome VI from coordinates 204996 to 204924.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.13882.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 22 25 + Hin6I GCGC 22 24 . . 22 25 + GlaI GCGC 23 23 . . 22 27 + Cac8I GCNNGC 24 24 . . 22 27 + BsePI GCGCGC 22 26 . . 22 25 + HhaI GCGC 24 22 . . 23 26 + FnuDII* CGCG 24 24 . . 24 27 + Hin6I GCGC 24 26 . . 24 27 + GlaI GCGC 25 25 . . 24 27 + HhaI GCGC 26 24 . . 26 36 + MwoI GCNNNNNNNGC 32 29 . . 30 36 + Bpu10I CCTNAGC 31 34 . . 30 40 + BsiYI* CCNNNNNNNGG 36 33 . . 31 35 + DdeI CTNAG 31 34 . . 36 39 + FatI CATG 35 39 . . 36 39 + NlaIII CATG 39 35 . . 36 39 + CviAII CATG 36 38 . . 44 47 + SetI ASST 47 43 . . 45 50 + Eco31I GGTCTC 51 55 . . 43 46 - MnlI CCTC 36 35 . . 46 50 + BsmAI GTCTC 51 55 . . 49 54 + BetI* WCCGGW 49 53 . . 50 53 + HpaII CCGG 50 52 . . 55 58 + TaqI TCGA 55 57 . . 57 61 + TfiI GAWTC 57 60 . . 57 61 + HinfI GANTC 57 60 . . 60 65 + Hpy178III* TCNNGA 61 63 . . 60 65 + BetI* WCCGGW 60 64 . . 60 65 + BspMII* TCCGGA 60 64 . . 61 64 + HpaII CCGG 61 63 . . 64 68 + HinfI GANTC 64 67 . . 64 68 - PleI GAGTC 58 59 . . 64 68 - MlyI GAGTC 58 58 . . #--------------------------------------- # # # BetI*: # [WCCGGW] # 11 13 49 # # Bpu10I: # [CCTNAGC] # 31 42 # # BsePI: # [GCGCGC] # 22 51 # # BsiYI*: # [CCNNNNNNNGG] # 36 37 # # BsmAI: # [GTCTC] # 22 51 # # BspMII*: # [TCCGGA] # 13 60 # # Cac8I: # [GCNNGC] # 24 49 # # CviAII: # [CATG] # 36 37 # # DdeI: # [CTNAG] # 31 42 # # Eco31I: # [GGTCTC] # 22 51 # # FatI: # [CATG] # 35 38 # # FnuDII*: # [CGCG] # 24 49 # # GlaI: # [GCGC] # 2 23 48 # # HhaI: # [GCGC] # 2 24 47 # # Hin6I: # [GCGC] # 2 22 49 # # HinfI: # [GANTC] # 7 9 57 # # HpaII: # [CCGG] # 11 12 50 # # Hpy178III*: # [TCNNGA] # 12 61 # # MlyI: # [GAGTC] # 15 58 # # MnlI: # [CCTC] # 36 37 # # MwoI: # [GCNNNNNNNGC] # 32 41 # # NlaIII: # [CATG] # 34 39 # # PleI: # [GAGTC] # 15 58 # # SetI: # [ASST] # 26 47 # # TaqI: # [TCGA] # 18 55 # # TfiI: # [GAWTC] # 16 57 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 73 # Reported_sequences: 1 # Reported_hitcount: 32 #---------------------------------------