Restriction Fragments for SNR86/snR86



Send questions or suggestions to SGD

BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

SNR86/snR86 on chromosome XIII from coordinates 763113 to 762110.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.5166.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 1 6 + TsoI TARCCA 17 15 . . 10 14 + TspDTI ATGAA 25 23 . . 13 18 + ApoI RAATTY 13 17 . . 14 17 + TspEI AATT 13 17 . . 27 31 - BslFI GGGAC 12 16 . . 31 41 + BsiYI* CCNNNNNNNGG 37 34 . . 33 36 + TspEI AATT 32 36 . . 38 42 + Hpy188I TCNGA 40 39 . . 50 53 + CviRI* TGCA 51 51 . . 49 54 - BsgI GTGCAG 34 32 . . 56 65 + XmnI GAANNNNTTC 60 60 . . 58 63 + SspI AATATT 60 60 . . 92 95 + SetI ASST 95 91 . . 92 95 + CviJI RGCY 93 93 . . 92 95 + AluI AGCT 93 93 . . 108 111 + TspEI AATT 107 111 . . 122 126 - MboII GAAGA 114 113 . . 131 135 - TspDTI ATGAA 121 119 . . 141 146 + MfeI CAATTG 141 145 . . 142 145 + TspEI AATT 141 145 . . 159 162 - MnlI CCTC 152 151 . . 163 168 + ApoI RAATTY 163 167 . . 164 167 + TspEI AATT 163 167 . . 177 180 + CviRI* TGCA 178 178 . . 192 197 + BtgZI GCGATG 207 211 . . 196 199 + CviRI* TGCA 197 197 . . 194 198 - SfaNI GCATC 184 188 . . 205 209 - TspDTI ATGAA 195 193 . . 211 214 + MseI TTAA 211 213 . . 234 244 + MwoI GCNNNNNNNGC 240 237 . . 237 241 + TspDTI ATGAA 252 250 . . 238 249 + BdaI TGANNNNNNTCA 227 225 261 259 239 248 + XmnI GAANNNNTTC 243 243 . . 241 246 + MluI ACGCGT 241 245 . . 241 246 + AflIII ACRYGT 241 245 . . 242 245 + FnuDII* CGCG 243 243 . . 238 249 - BdaI TGANNNNNNTCA 261 259 227 225 246 250 - TspDTI ATGAA 236 234 . . 253 256 + MseI TTAA 253 255 . . 257 261 + Tsp45I GTSAC 256 261 . . 257 261 + MaeIII GTNAC 256 261 . . 260 265 + BetI* WCCGGW 260 264 . . 261 264 + HpaII CCGG 261 263 . . 263 268 + NlaIV GGNNCC 265 265 . . 263 267 + BmgT120I GGNCC 264 265 . . 263 267 + AvaII GGWCC 263 266 . . 263 267 + AsuI* GGNCC 263 266 . . 272 277 + SspI AATATT 274 274 . . 279 282 + SetI ASST 282 278 . . 285 288 + FatI CATG 284 288 . . 285 288 + NlaIII CATG 288 284 . . 285 288 + CviAII CATG 285 287 . . 289 293 + TspGWI ACGGA 304 302 . . 294 297 + SetI ASST 297 293 . . 294 297 + CviJI RGCY 295 295 . . 294 297 + AluI AGCT 295 295 . . 298 302 + TspDTI ATGAA 313 311 . . 301 304 + TspEI AATT 300 304 . . 302 307 + VspI ATTAAT 303 305 . . 303 306 + MseI TTAA 303 305 . . 308 318 + EcoNI CCTNNNNNAGG 312 313 . . 308 312 + BssKI CCNGG 307 312 . . 308 312 + BseBI CCWGG 309 310 . . 308 312 + ScrFI CCNGG 309 310 . . 308 312 + EcoRII CCWGG 307 312 . . 308 318 + BsiYI* CCNNNNNNNGG 314 311 . . 315 318 - MnlI CCTC 308 307 . . 333 336 - MnlI CCTC 326 325 . . 339 343 + FokI GGATG 352 356 . . 339 343 + BseGI GGATG 345 343 . . 337 340 - MnlI CCTC 330 329 . . 354 358 + TspGWI ACGGA 369 367 . . 362 365 + CviRI* TGCA 363 363 . . 371 374 + TaiI ACGT 374 370 . . 371 374 + SetI ASST 374 370 . . 371 374 + MaeII ACGT 371 373 . . 368 372 - BsmAI GTCTC 362 366 . . 368 373 - Esp3I CGTCTC 362 366 . . 411 414 - AciI CCGC 411 413 . . 421 424 + TaiI ACGT 424 420 . . 421 424 + SetI ASST 424 420 . . 421 424 + MaeII ACGT 421 423 . . 452 455 + SetI ASST 455 451 . . 474 478 + TfiI GAWTC 474 477 . . 474 478 + HinfI GANTC 474 477 . . 483 487 + TfiI GAWTC 483 486 . . 483 487 + HinfI GANTC 483 486 . . 488 493 + AflIII ACRYGT 488 492 . . 490 493 + TaiI ACGT 493 489 . . 490 493 + SetI ASST 493 489 . . 490 493 + MaeII ACGT 490 492 . . 512 517 + Hpy166II GTNNAC 514 514 . . 512 517 + HpaI GTTAAC 514 514 . . 512 517 + HindII GTYRAC 514 514 . . 513 516 + MseI TTAA 513 515 . . 516 520 + TspGWI ACGGA 531 529 . . 516 526 + BsaXI ACNNNNNCTCC 506 503 536 533 525 535 + BsiYI* CCNNNNNNNGG 531 528 . . 526 529 + FatI CATG 525 529 . . 526 529 + NlaIII CATG 529 525 . . 526 529 + CviAII CATG 526 528 . . 530 533 + MseI TTAA 530 532 . . 533 536 + SetI ASST 536 532 . . 540 545 + PsiI TTATAA 542 542 . . 548 553 + ApoI RAATTY 548 552 . . 549 552 + TspEI AATT 548 552 . . 557 566 + XmnI GAANNNNTTC 561 561 . . 557 562 + ApoI RAATTY 557 561 . . 558 561 + TspEI AATT 557 561 . . 578 583 + Hpy166II GTNNAC 580 580 . . 587 590 + MnlI CCTC 597 596 . . 589 594 + Hpy178III* TCNNGA 590 592 . . 585 590 - BseRI GAGGAG 576 574 . . 588 593 - BsiI* CACGAG 588 592 . . 619 622 + MnlI CCTC 629 628 . . 616 620 - FokI GGATG 602 606 . . 616 620 - BseGI GGATG 615 613 . . 623 627 + Hpy188I TCNGA 625 624 . . 642 645 + FatI CATG 641 645 . . 642 645 + NlaIII CATG 645 641 . . 642 645 + CviAII CATG 642 644 . . 645 648 + CviJI RGCY 646 646 . . 653 657 - TspDTI ATGAA 643 641 . . 655 660 - BsrDI GCAATG 654 652 . . 667 671 + Tsp4CI* ACNGT 669 668 . . 672 676 - BccI CCATC 666 667 . . 692 695 + CviRI* TGCA 693 693 . . 699 703 + TspDTI ATGAA 714 712 . . 703 706 + TaiI ACGT 706 702 . . 703 706 + SetI ASST 706 702 . . 703 706 + MaeII ACGT 703 705 . . 712 715 + SetI ASST 715 711 . . 711 714 - MnlI CCTC 704 703 . . 733 738 + Hpy166II GTNNAC 735 735 . . 734 739 + TatI WGTACW 734 738 . . 735 738 + Csp6I GTAC 735 737 . . 735 738 + RsaI GTAC 736 736 . . 738 743 + SfeI* CTRYAG 738 742 . . 744 747 + CviRI* TGCA 745 745 . . 745 750 + BsrDI GCAATG 752 750 . . 756 759 + MseI TTAA 756 758 . . 758 761 + TspEI AATT 757 761 . . 778 781 + TaqI TCGA 778 780 . . 775 779 - MboII GAAGA 767 766 . . 790 793 - AciI CCGC 790 792 . . 788 793 - BsrBI CCGCTC 790 790 . . 793 796 - MnlI CCTC 786 785 . . 801 804 + SetI ASST 804 800 . . 802 806 + HphI GGTGA 814 813 . . 799 803 - Hin4II* CCTTC 793 792 . . 807 810 + TspEI AATT 806 810 . . 813 817 - Hin4II* CCTTC 807 806 . . 827 837 + Hin4I GAYNNNNNVTC 818 813 850 845 836 841 + Hpy178III* TCNNGA 837 839 . . 827 837 - Hin4I GAYNNNNNVTC 850 845 818 813 842 846 + TspDTI ATGAA 857 855 . . 878 881 + CviRI* TGCA 879 879 . . 879 884 + NspI RCATGY 883 879 . . 880 883 + FatI CATG 879 883 . . 880 883 + NlaIII CATG 883 879 . . 880 883 + CviAII CATG 880 882 . . 908 911 + TaqI TCGA 908 910 . . 904 909 - BsmI GAATGC 904 902 . . 914 919 + Hpy166II GTNNAC 916 916 . . 910 914 - BccI CCATC 904 905 . . 929 934 + ApoI RAATTY 929 933 . . 930 933 + TspEI AATT 929 933 . . 946 949 + MseI TTAA 946 948 . . 961 964 + MseI TTAA 961 963 . . 985 989 - TspDTI ATGAA 975 973 . . 991 996 + SmaI CCCGGG 993 993 . . 991 996 + Cfr9I CCCGGG 991 995 . . 991 995 + BssKI CCNGG 990 995 . . 991 996 + SecI* CCNNGG 991 995 . . 991 996 + BmeT110I CYCGRG 992 994 . . 991 995 + ScrFI CCNGG 992 993 . . 991 995 + CauII* CCSGG 992 993 . . 991 996 + AvaI CYCGRG 991 995 . . 992 996 + BssKI CCNGG 991 996 . . 992 995 + HpaII CCGG 992 994 . . 992 996 + ScrFI CCNGG 993 994 . . 992 996 + CauII* CCSGG 993 994 . . 999 1004 + ApoI RAATTY 999 1003 . . 1000 1003 + TspEI AATT 999 1003 . . #--------------------------------------- # # # AciI: # [CCGC] # 214 379 411 # # AflIII: # [ACRYGT] # 241 247 516 # # AluI: # [AGCT] # 93 202 709 # # ApoI: # [RAATTY] # 5 9 13 70 150 372 # 385 # # AsuI*: # [GGNCC] # 263 741 # # AvaI: # [CYCGRG] # 13 991 # # AvaII: # [GGWCC] # 263 741 # # BccI: # [CCATC] # 100 238 666 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 227 743 # # BetI*: # [WCCGGW] # 260 744 # # BmeT110I: # [CYCGRG] # 12 992 # # BmgT120I: # [GGNCC] # 264 740 # # BsaXI: # [ACNNNNNCTCC] # 30 468 506 # # BseBI: # [CCWGG] # 309 695 # # BseGI: # [GGATG] # 270 345 389 # # BseRI: # [GAGGAG] # 428 576 # # BsgI: # [GTGCAG] # 34 970 # # BsiI*: # [CACGAG] # 416 588 # # BsiYI*: # [CCNNNNNNNGG] # 37 217 277 473 # # BslFI: # [GGGAC] # 12 992 # # BsmAI: # [GTCTC] # 362 642 # # BsmI: # [GAATGC] # 100 904 # # BsrBI: # [CCGCTC] # 214 790 # # BsrDI: # [GCAATG] # 98 252 654 # # BssKI: # [CCNGG] # 1 13 307 683 # # BtgZI: # [GCGATG] # 207 797 # # CauII*: # [CCSGG] # 1 11 992 # # Cfr9I: # [CCCGGG] # 13 991 # # Csp6I: # [GTAC] # 269 735 # # CviAII: # [CATG] # 116 124 238 241 285 # # CviJI: # [RGCY] # 93 202 351 358 # # CviRI*: # [TGCA] # 19 51 52 125 127 134 # 166 330 # # EcoNI: # [CCTNNNNNAGG] # 312 692 # # EcoRII: # [CCWGG] # 307 697 # # Esp3I: # [CGTCTC] # 362 642 # # FatI: # [CATG] # 116 125 238 241 284 # # FnuDII*: # [CGCG] # 243 761 # # FokI: # [GGATG] # 250 352 402 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 154 818 # # Hin4II*: # [CCTTC] # 14 197 793 # # HindII: # [GTYRAC] # 490 514 # # HinfI: # [GANTC] # 9 474 521 # # HpaI: # [GTTAAC] # 490 514 # # HpaII: # [CCGG] # 12 261 731 # # HphI: # [GGTGA] # 190 814 # # Hpy166II: # [GTNNAC] # 66 88 155 181 514 # # Hpy178III*: # [TCNNGA] # 167 247 590 # # Hpy188I: # [TCNGA] # 40 379 585 # # MaeII: # [ACGT] # 50 69 213 301 371 # # MaeIII: # [GTNAC] # 256 748 # # MboII: # [GAAGA] # 114 237 653 # # MfeI: # [CAATTG] # 141 863 # # MluI: # [ACGCGT] # 241 763 # # MnlI: # [CCTC] # 4 18 32 75 82 152 # 156 218 267 # # MseI: # [TTAA] # 15 17 42 43 50 190 # 210 211 226 # # MwoI: # [GCNNNNNNNGC] # 240 764 # # NlaIII: # [CATG] # 116 121 238 241 288 # # NlaIV: # [GGNNCC] # 265 739 # # NspI: # [RCATGY] # 121 883 # # PsiI: # [TTATAA] # 462 542 # # RsaI: # [GTAC] # 268 736 # # ScrFI: # [CCNGG] # 1 11 309 683 # # SecI*: # [CCNNGG] # 13 991 # # SetI: # [ASST] # 9 15 31 38 43 50 # 77 89 95 170 187 200 # # SfaNI: # [GCATC] # 184 820 # # SfeI*: # [CTRYAG] # 266 738 # # SmaI: # [CCCGGG] # 11 993 # # SspI: # [AATATT] # 60 214 730 # # TaiI: # [ACGT] # 50 69 213 298 374 # # TaqI: # [TCGA] # 96 130 778 # # TatI: # [WGTACW] # 270 734 # # TfiI: # [GAWTC] # 9 474 521 # # TsoI: # [TARCCA] # 17 987 # # Tsp45I: # [GTSAC] # 256 748 # # Tsp4CI*: # [ACNGT] # 335 669 # # TspDTI: # [ATGAA] # 16 25 29 41 61 71 # 74 96 118 143 330 # # TspEI: # [AATT] # 5 9 13 19 22 34 # 49 70 75 123 137 200 # 248 # # TspGWI: # [ACGGA] # 65 162 304 473 # # VspI: # [ATTAAT] # 303 701 # # XmnI: # [GAANNNNTTC] # 60 183 318 443 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1004 # Reported_sequences: 1 # Reported_hitcount: 184 #---------------------------------------