Restriction Fragments for SNR85/snR85



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The currently selected gene/sequence is :

SNR85/snR85 on chromosome XIII from coordinates 67938 to 67768.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.15146.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 6 + MaeI CTAG 3 5 . . 9 14 + NdeI CATATG 10 12 . . 17 22 + ApoI RAATTY 17 21 . . 18 21 + TspEI AATT 17 21 . . 25 28 + TspEI AATT 24 28 . . 27 30 + MseI TTAA 27 29 . . 29 34 + AclI AACGTT 30 32 . . 30 33 + TaiI ACGT 33 29 . . 30 33 + SetI ASST 33 29 . . 30 33 + MaeII ACGT 30 32 . . 42 46 - TspDTI ATGAA 32 30 . . 52 56 + DdeI CTNAG 52 55 . . 57 60 + CviJI RGCY 58 58 . . 58 62 - BceAI ACGGC 43 45 . . 63 66 + TspEI AATT 62 66 . . 72 77 + EcoRV GATATC 74 74 . . 87 91 + MboII GAAGA 99 98 . . 97 107 + BsiYI* CCNNNNNNNGG 103 100 . . 105 108 + SetI ASST 108 104 . . 104 107 - MnlI CCTC 97 96 . . 113 118 + TatI WGTACW 113 117 . . 114 119 + Hpy166II GTNNAC 116 116 . . 114 117 + Csp6I GTAC 114 116 . . 114 117 + RsaI GTAC 115 115 . . 124 129 + McrI* CGRYCG 127 125 . . 124 129 + PvuI CGATCG 127 125 . . 124 135 + BcgI CGANNNNNNTGC 113 111 147 145 125 128 + DpnI GATC 126 126 . . 125 128 + BstKTI GATC 127 125 . . 125 128 + MboI GATC 124 128 . . 132 137 + SduI GDGCHC 136 132 . . 132 137 + HgiAI* GWGCWC 136 132 . . 134 144 + MwoI GCNNNNNNNGC 140 137 . . 138 141 + MnlI CCTC 148 147 . . 138 144 + Bpu10I CCTNAGC 139 142 . . 138 144 + BbvCI CCTCAGC 139 142 . . 139 143 + DdeI CTNAG 139 142 . . 139 143 + BspCNI CTCAG 152 150 . . 139 143 + BseMII CTCAG 153 151 . . 142 145 + CviJI RGCY 143 143 . . 144 149 + DsaI* CCRYGG 144 148 . . 144 149 + SecI* CCNNGG 144 148 . . 145 154 + MslI CAYNNNNRTG 149 149 . . 145 154 + OliI CACNNNNGTG 149 149 . . 150 155 + Cac8I GCNNGC 152 152 . . #--------------------------------------- # # # AclI: # [AACGTT] # 30 141 # # ApoI: # [RAATTY] # 17 154 # # BbvCI: # [CCTCAGC] # 32 139 # # BceAI: # [ACGGC] # 43 128 # # BcgI: # [CGANNNNNNTGC] # 24 34 113 # # Bpu10I: # [CCTNAGC] # 32 139 # # BseMII: # [CTCAG] # 18 153 # # BsiYI*: # [CCNNNNNNNGG] # 68 103 # # BspCNI: # [CTCAG] # 19 152 # # BstKTI: # [GATC] # 44 127 # # Cac8I: # [GCNNGC] # 19 152 # # Csp6I: # [GTAC] # 57 114 # # CviJI: # [RGCY] # 28 58 85 # # DdeI: # [CTNAG] # 32 52 87 # # DpnI: # [GATC] # 45 126 # # DsaI*: # [CCRYGG] # 27 144 # # EcoRV: # [GATATC] # 74 97 # # HgiAI*: # [GWGCWC] # 35 136 # # Hpy166II: # [GTNNAC] # 55 116 # # MaeI: # [CTAG] # 3 168 # # MaeII: # [ACGT] # 30 141 # # MboI: # [GATC] # 47 124 # # MboII: # [GAAGA] # 72 99 # # McrI*: # [CGRYCG] # 44 127 # # MnlI: # [CCTC] # 23 51 97 # # MseI: # [TTAA] # 27 144 # # MslI: # [CAYNNNNRTG] # 22 149 # # MwoI: # [GCNNNNNNNGC] # 31 140 # # NdeI: # [CATATG] # 10 161 # # OliI: # [CACNNNNGTG] # 22 149 # # PvuI: # [CGATCG] # 44 127 # # RsaI: # [GTAC] # 56 115 # # SduI: # [GDGCHC] # 35 136 # # SecI*: # [CCNNGG] # 27 144 # # SetI: # [ASST] # 33 63 75 # # TaiI: # [ACGT] # 33 138 # # TatI: # [WGTACW] # 58 113 # # TspDTI: # [ATGAA] # 32 139 # # TspEI: # [AATT] # 7 17 38 109 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 171 # Reported_sequences: 1 # Reported_hitcount: 45 #---------------------------------------