Restriction Fragments for SNR19/snR19



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The currently selected gene/sequence is :

SNR19/snR19 on chromosome XIV from coordinates 230672 to 230105.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.29947.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 7 10 + SetI ASST 10 6 . . 9 14 + SmlI CTYRAG 9 13 . . 9 14 + AflII CTTAAG 9 13 . . 10 13 + MseI TTAA 10 12 . . 14 19 + EcoRV GATATC 16 16 . . 18 22 + Hpy188I TCNGA 20 19 . . 21 26 + BseRI GAGGAG 36 34 . . 21 24 - MnlI CCTC 14 13 . . 26 29 + DpnI GATC 27 27 . . 26 29 + BstKTI GATC 28 26 . . 26 29 + MboI GATC 25 29 . . 28 33 + Hpy178III* TCNNGA 29 31 . . 42 47 + BclI TGATCA 42 46 . . 43 46 + DpnI GATC 44 44 . . 43 46 + BstKTI GATC 45 43 . . 43 46 + MboI GATC 42 46 . . 49 54 + NspI RCATGY 53 49 . . 50 53 + FatI CATG 49 53 . . 50 53 + NlaIII CATG 53 49 . . 50 53 + CviAII CATG 50 52 . . 53 56 + Hin6I GCGC 53 55 . . 53 56 + GlaI GCGC 54 54 . . 53 56 + HhaI GCGC 55 53 . . 68 72 - Hin4II* CCTTC 62 61 . . 73 76 + TaiI ACGT 76 72 . . 73 76 + SetI ASST 76 72 . . 73 76 + MaeII ACGT 73 75 . . 76 79 + MseI TTAA 76 78 . . 99 102 + TspEI AATT 98 102 . . 104 108 - TspDTI ATGAA 94 92 . . 119 129 + Hin4I GAYNNNNNVTC 110 105 142 137 121 124 + CviRI* TGCA 122 122 . . 119 123 - SfaNI GCATC 109 113 . . 129 134 + StyI CCWWGG 129 133 . . 129 134 + SecI* CCNNGG 129 133 . . 134 138 + Tsp45I GTSAC 133 138 . . 134 138 + MaeIII GTNAC 133 138 . . 147 151 + BceAI ACGGC 163 165 . . 150 153 + Hin6I GCGC 150 152 . . 150 153 + GlaI GCGC 151 151 . . 150 160 + MwoI GCNNNNNNNGC 156 153 . . 150 153 + HhaI GCGC 152 150 . . 151 154 + FnuDII* CGCG 152 152 . . 152 155 - AciI CCGC 152 154 . . 155 159 - Hin4II* CCTTC 149 148 . . 171 174 + TaiI ACGT 174 170 . . 171 174 + SetI ASST 174 170 . . 171 174 + MaeII ACGT 171 173 . . 201 204 + MseI TTAA 201 203 . . 198 203 - TsoI TARCCA 188 186 . . 211 215 + TfiI GAWTC 211 214 . . 211 215 + HinfI GANTC 211 214 . . 233 236 + MseI TTAA 233 235 . . 247 253 + DraII RGGNCCY 248 251 . . 247 252 + NlaIV GGNNCC 249 249 . . 248 254 + DraII RGGNCCY 249 252 . . 248 253 + SduI GDGCHC 252 248 . . 248 253 + Bsp120I GGGCCC 248 252 . . 248 253 + NlaIV GGNNCC 250 250 . . 248 252 + BmgT120I GGNCC 249 250 . . 248 253 + HgiJII* GRGCYC 252 248 . . 248 253 + BseSI GKGCMC 252 248 . . 248 252 + AsuI* GGNCC 248 251 . . 248 253 + ApaI GGGCCC 252 248 . . 249 252 + CviJI RGCY 250 250 . . 249 252 + HaeIII GGCC 250 250 . . 249 253 + BmgT120I GGNCC 250 251 . . 249 253 + AsuI* GGNCC 249 252 . . 260 264 - MboII GAAGA 252 251 . . 267 271 + BssKI CCNGG 266 271 . . 267 271 + BseBI CCWGG 268 269 . . 267 271 + ScrFI CCNGG 268 269 . . 267 271 + EcoRII CCWGG 266 271 . . 268 273 + Eco57MI CTGRAG 289 287 . . 268 273 + GsuI CTGGAG 289 287 . . 284 298 + XcmI CCANNNNNNNNNTGG 291 290 . . 286 291 + ApoI RAATTY 286 290 . . 287 290 + TspEI AATT 286 290 . . 293 296 + TspEI AATT 292 296 . . 308 311 + SetI ASST 311 307 . . 308 311 + CviJI RGCY 309 309 . . 308 311 + AluI AGCT 309 309 . . 312 318 + DraII RGGNCCY 313 316 . . 312 317 + NlaIV GGNNCC 314 314 . . 313 317 + BmgT120I GGNCC 314 315 . . 313 317 + AsuI* GGNCC 313 316 . . 309 314 - GsaI CCCAGC 313 309 . . 314 317 + CviJI RGCY 315 315 . . 314 317 + HaeIII GGCC 315 315 . . 309 314 - BseYI CCCAGC 309 313 . . 329 332 + SetI ASST 332 328 . . 329 332 + CviJI RGCY 330 330 . . 329 332 + AluI AGCT 330 330 . . 338 341 - MnlI CCTC 331 330 . . 341 346 - BsmI GAATGC 341 339 . . 364 367 + MaeI CTAG 364 366 . . 367 372 + Cac8I GCNNGC 369 369 . . 387 391 + BceAI ACGGC 403 405 . . 393 397 + TfiI GAWTC 393 396 . . 393 397 + HinfI GANTC 393 396 . . 395 400 + AsuII TTCGAA 396 398 . . 396 399 + TaqI TCGA 396 398 . . 400 404 + TspDTI ATGAA 415 413 . . 405 410 + SmlI CTYRAG 405 409 . . 405 410 + AflII CTTAAG 405 409 . . 406 409 + MseI TTAA 406 408 . . 417 421 + TspDTI ATGAA 432 430 . . 421 424 + SetI ASST 424 420 . . 419 423 - Hin4II* CCTTC 413 412 . . 427 430 + CviJI RGCY 428 428 . . 446 451 + Hpy178III* TCNNGA 447 449 . . 461 465 + MboII GAAGA 473 472 . . 467 472 + SduI GDGCHC 471 467 . . 467 472 + HgiAI* GWGCWC 471 467 . . 476 487 + BdaI TGANNNNNNTCA 465 463 499 497 482 486 + BsmAI GTCTC 487 491 . . 476 487 - BdaI TGANNNNNNTCA 499 497 465 463 500 510 + BsiYI* CCNNNNNNNGG 506 503 . . 513 518 + ApoI RAATTY 513 517 . . 514 517 + TspEI AATT 513 517 . . 523 526 + SetI ASST 526 522 . . 521 525 - Hin4II* CCTTC 515 514 . . 543 548 + XhoII RGATCY 543 547 . . 543 547 + BinI* GGATC 551 552 . . 544 547 + DpnI GATC 545 545 . . 544 547 + BstKTI GATC 546 544 . . 544 547 + MboI GATC 543 547 . . 548 553 + PsiI TTATAA 550 550 . . 552 555 + TspEI AATT 551 555 . . #--------------------------------------- # # # AciI: # [CCGC] # 152 416 # # AflII: # [CTTAAG] # 9 163 396 # # AluI: # [AGCT] # 21 238 309 # # ApaI: # [GGGCCC] # 252 316 # # ApoI: # [RAATTY] # 55 227 286 # # AsuI*: # [GGNCC] # 1 64 248 255 # # AsuII: # [TTCGAA] # 172 396 # # BceAI: # [ACGGC] # 163 165 240 # # BclI: # [TGATCA] # 42 526 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 69 465 # # BinI*: # [GGATC] # 17 551 # # BmgT120I: # [GGNCC] # 1 64 249 254 # # BseBI: # [CCWGG] # 268 300 # # BseRI: # [GAGGAG] # 36 532 # # BseSI: # [GKGCMC] # 252 316 # # BseYI: # [CCCAGC] # 259 309 # # BsiYI*: # [CCNNNNNNNGG] # 62 506 # # BsmAI: # [GTCTC] # 81 487 # # BsmI: # [GAATGC] # 227 341 # # Bsp120I: # [GGGCCC] # 248 320 # # BssKI: # [CCNGG] # 266 302 # # BstKTI: # [GATC] # 17 22 28 501 # # Cac8I: # [GCNNGC] # 199 369 # # CviAII: # [CATG] # 50 518 # # CviJI: # [RGCY] # 6 15 59 98 140 250 # # CviRI*: # [TGCA] # 122 446 # # DpnI: # [GATC] # 17 23 27 501 # # DraII: # [RGGNCCY] # 1 64 248 255 # # Eco57MI: # [CTGRAG] # 279 289 # # EcoRII: # [CCWGG] # 266 302 # # EcoRV: # [GATATC] # 16 552 # # FatI: # [CATG] # 49 519 # # FnuDII*: # [CGCG] # 152 416 # # GlaI: # [GCGC] # 54 97 417 # # GsaI: # [CCCAGC] # 255 313 # # GsuI: # [CTGGAG] # 279 289 # # HaeIII: # [GGCC] # 65 250 253 # # HgiAI*: # [GWGCWC] # 97 471 # # HgiJII*: # [GRGCYC] # 252 316 # # HhaI: # [GCGC] # 55 97 416 # # Hin4I: # [GAYNNNNNVTC] # 32 110 426 # # Hin4II*: # [CCTTC] # 53 62 87 102 264 # # Hin6I: # [GCGC] # 53 97 418 # # HinfI: # [GANTC] # 175 182 211 # # Hpy178III*: # [TCNNGA] # 29 121 418 # # Hpy188I: # [TCNGA] # 20 548 # # MaeI: # [CTAG] # 204 364 # # MaeII: # [ACGT] # 73 98 397 # # MaeIII: # [GTNAC] # 133 435 # # MboI: # [GATC] # 17 25 25 501 # # MboII: # [GAAGA] # 95 221 252 # # MnlI: # [CCTC] # 14 237 317 # # MseI: # [TTAA] # 10 32 66 125 162 173 # # MwoI: # [GCNNNNNNNGC] # 156 412 # # NlaIII: # [CATG] # 53 515 # # NlaIV: # [GGNNCC] # 1 64 249 254 # # NspI: # [RCATGY] # 53 515 # # PsiI: # [TTATAA] # 18 550 # # ScrFI: # [CCNGG] # 268 300 # # SduI: # [GDGCHC] # 97 219 252 # # SecI*: # [CCNNGG] # 129 439 # # SetI: # [ASST] # 10 21 42 66 92 98 # 102 137 # # SfaNI: # [GCATC] # 109 459 # # SmlI: # [CTYRAG] # 9 163 396 # # StyI: # [CCWWGG] # 129 439 # # TaiI: # [ACGT] # 76 98 394 # # TaqI: # [TCGA] # 172 396 # # TfiI: # [GAWTC] # 175 182 211 # # TsoI: # [TARCCA] # 188 380 # # Tsp45I: # [GTSAC] # 133 435 # # TspDTI: # [ATGAA] # 17 94 136 321 # # TspEI: # [AATT] # 6 17 38 98 188 221 # # XcmI: # [CCANNNNNNNNNTGG] # 277 291 # # XhoII: # [RGATCY] # 25 543 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 568 # Reported_sequences: 1 # Reported_hitcount: 129 #---------------------------------------