Restriction Fragments for YPRCdelta24



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The currently selected gene/sequence is :

YPRCdelta24 on chromosome XVI from coordinates 856554 to 856217.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.9740.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 65 69 + Tsp4CI* ACNGT 67 66 . . 71 74 + MseI TTAA 71 73 . . 97 100 + SetI ASST 100 96 . . 97 100 + CviJI RGCY 98 98 . . 97 100 + AluI AGCT 98 98 . . 93 103 - Hin4I GAYNNNNNVTC 116 111 84 79 104 109 + ClaI ATCGAT 105 107 . . 105 108 + TaqI TCGA 105 107 . . 114 117 - MnlI CCTC 107 106 . . 119 122 + SetI ASST 122 118 . . 119 122 + CviJI RGCY 120 120 . . 119 122 + AluI AGCT 120 120 . . 149 153 + BinI* GGATC 157 158 . . 150 153 + DpnI GATC 151 151 . . 150 153 + BstKTI GATC 152 150 . . 150 153 + MboI GATC 149 153 . . 155 159 + TspDTI ATGAA 170 168 . . 174 178 + TspGWI ACGGA 189 187 . . 181 184 - MnlI CCTC 174 173 . . 181 191 - Hin4I GAYNNNNNVTC 204 199 172 167 194 199 + SspI AATATT 196 196 . . 216 220 + TfiI GAWTC 216 219 . . 216 220 + HinfI GANTC 216 219 . . 220 230 + BsiYI* CCNNNNNNNGG 226 223 . . 230 234 + TfiI GAWTC 230 233 . . 227 230 - MnlI CCTC 220 219 . . 230 234 + HinfI GANTC 230 233 . . 241 248 + PspXI VCTCGAGB 242 246 . . 241 244 + MnlI CCTC 251 250 . . 241 248 + AbsI CCTCGAGG 242 246 . . 242 247 + SmlI CTYRAG 242 246 . . 242 247 + XhoI CTCGAG 242 246 . . 242 247 + BmeT110I CYCGRG 243 245 . . 242 247 + AvaI CYCGRG 242 246 . . 243 246 + TaqI TCGA 243 245 . . 245 250 + BseRI GAGGAG 260 258 . . 245 248 - MnlI CCTC 238 237 . . 256 259 + MaeI CTAG 256 258 . . 268 273 + Hpy166II GTNNAC 270 270 . . 268 273 + BssNAI GTATAC 270 270 . . 268 273 + AccI GTMKAC 269 271 . . 272 275 + SetI ASST 275 271 . . 276 281 + SspI AATATT 278 278 . . 284 287 + CviJI RGCY 285 285 . . 301 305 + TfiI GAWTC 301 304 . . 301 305 + HinfI GANTC 301 304 . . 311 314 + TspEI AATT 310 314 . . 319 322 + TspEI AATT 318 322 . . 325 329 - HphI GGTGA 317 316 . . #--------------------------------------- # # # AbsI: # [CCTCGAGG] # 96 242 # # AccI: # [GTMKAC] # 69 269 # # AluI: # [AGCT] # 22 98 218 # # AvaI: # [CYCGRG] # 96 242 # # BinI*: # [GGATC] # 157 181 # # BmeT110I: # [CYCGRG] # 95 243 # # BseRI: # [GAGGAG] # 78 260 # # BsiYI*: # [CCNNNNNNNGG] # 112 226 # # BssNAI: # [GTATAC] # 68 270 # # BstKTI: # [GATC] # 152 186 # # ClaI: # [ATCGAT] # 105 233 # # CviJI: # [RGCY] # 22 53 98 165 # # DpnI: # [GATC] # 151 187 # # Hin4I: # [GAYNNNNNVTC] # 32 32 56 84 134 # # HinfI: # [GANTC] # 14 37 71 216 # # HphI: # [GGTGA] # 21 317 # # Hpy166II: # [GTNNAC] # 68 270 # # MaeI: # [CTAG] # 82 256 # # MboI: # [GATC] # 149 189 # # MnlI: # [CCTC] # 13 18 46 67 87 107 # # MseI: # [TTAA] # 71 267 # # PspXI: # [VCTCGAGB] # 96 242 # # SetI: # [ASST] # 22 63 100 153 # # SmlI: # [CTYRAG] # 96 242 # # SspI: # [AATATT] # 60 82 196 # # TaqI: # [TCGA] # 95 105 138 # # TfiI: # [GAWTC] # 14 37 71 216 # # Tsp4CI*: # [ACNGT] # 67 271 # # TspDTI: # [ATGAA] # 168 170 # # TspEI: # [AATT] # 8 20 310 # # TspGWI: # [ACGGA] # 149 189 # # XhoI: # [CTCGAG] # 96 242 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 338 # Reported_sequences: 1 # Reported_hitcount: 49 #---------------------------------------