Restriction Fragments for SUA7/YPR086W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

SUA7/YPR086W on chromosome XVI from coordinates 710101 to 711138.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.7716.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 8 11 + MaeI CTAG 8 10 . . 35 39 + MboII GAAGA 47 46 . . 42 48 + PpuMI RGGWCCY 43 46 . . 38 42 - Hin4II* CCTTC 32 31 . . 42 48 + DraII RGGNCCY 43 46 . . 42 47 + NlaIV GGNNCC 44 44 . . 43 47 + BmgT120I GGNCC 44 45 . . 43 47 + AvaII GGWCC 43 46 . . 43 47 + AsuI* GGNCC 43 46 . . 51 55 + Hpy188I TCNGA 53 52 . . 55 60 + SspI AATATT 57 57 . . 67 70 + TaiI ACGT 70 66 . . 67 70 + SetI ASST 70 66 . . 67 70 + MaeII ACGT 67 69 . . 67 72 + AflIII ACRYGT 67 71 . . 66 71 - BtrI CACGTC 68 68 . . 72 77 + Hpy178III* TCNNGA 73 75 . . 72 77 + BetI* WCCGGW 72 76 . . 72 77 + BspMII* TCCGGA 72 76 . . 73 76 + HpaII CCGG 73 75 . . 79 82 + CviRI* TGCA 80 80 . . 85 90 + Hpy166II GTNNAC 87 87 . . 85 90 + AccI GTMKAC 86 88 . . 118 122 - Hin4II* CCTTC 112 111 . . 123 127 + FokI GGATG 136 140 . . 123 127 + BseGI GGATG 129 127 . . 134 139 + SduI GDGCHC 138 134 . . 134 139 + HgiAI* GWGCWC 138 134 . . 148 151 + MaeI CTAG 148 150 . . 150 155 + TatI WGTACW 150 154 . . 150 155 + ScaI AGTACT 152 152 . . 151 154 + Csp6I GTAC 151 153 . . 151 154 + RsaI GTAC 152 152 . . 157 161 + Hpy188I TCNGA 159 158 . . 165 169 + BsrI ACTGG 170 168 . . 169 174 + Hpy166II GTNNAC 171 171 . . 169 174 + HindII GTYRAC 171 171 . . 172 182 + Hin4I GAYNNNNNVTC 163 158 195 190 172 182 + Eam1105I GACNNNNNGTC 177 176 . . 175 181 + SexAI ACCWGGT 175 180 . . 176 180 + BssKI CCNGG 175 180 . . 176 180 + BseBI CCWGG 177 178 . . 176 180 + ScrFI CCNGG 177 178 . . 176 180 + EcoRII CCWGG 175 180 . . 178 181 + SetI ASST 181 177 . . 181 185 + Hpy188I TCNGA 183 182 . . 172 182 - Hin4I GAYNNNNNVTC 195 190 163 158 180 185 - MmeI TCCRAC 161 159 . . 191 200 + XmnI GAANNNNTTC 195 195 . . 192 197 + AclI AACGTT 193 195 . . 193 196 + TaiI ACGT 196 192 . . 193 196 + SetI ASST 196 192 . . 193 196 + MaeII ACGT 193 195 . . 207 212 + BclI TGATCA 207 211 . . 208 211 + DpnI GATC 209 209 . . 208 211 + BstKTI GATC 210 208 . . 208 211 + MboI GATC 207 211 . . 211 220 + MslI CAYNNNNRTG 215 215 . . 211 220 + OliI CACNNNNGTG 215 215 . . 215 219 + Tsp4CI* ACNGT 217 216 . . 217 221 + HphI GGTGA 229 228 . . 223 232 + PshAI GACNNNNGTC 227 227 . . 238 242 + HphI GGTGA 250 249 . . 243 246 + CviJI RGCY 244 244 . . 241 244 - MnlI CCTC 234 233 . . 253 256 + MnlI CCTC 263 262 . . 262 266 - BccI CCATC 256 257 . . 272 275 + SetI ASST 275 271 . . 284 288 + BinI* GGATC 292 293 . . 285 288 + DpnI GATC 286 286 . . 285 288 + BstKTI GATC 287 285 . . 285 288 + MboI GATC 284 288 . . 287 291 + Hpy188I TCNGA 289 288 . . 295 299 + HphI GGTGA 307 306 . . 302 307 + DsaI* CCRYGG 302 306 . . 302 307 + SecI* CCNNGG 302 306 . . 303 312 + MslI CAYNNNNRTG 307 307 . . 304 308 + TspGWI ACGGA 319 317 . . 314 318 + TfiI GAWTC 314 317 . . 314 318 + HinfI GANTC 314 317 . . 317 321 - HphI GGTGA 309 308 . . 343 354 - TstI CACNNNNNNTCC 367 362 335 330 354 358 - BccI CCATC 348 349 . . 371 375 + TspDTI ATGAA 386 384 . . 375 380 + TatI WGTACW 375 379 . . 376 379 + Csp6I GTAC 376 378 . . 376 379 + RsaI GTAC 377 377 . . 381 384 + SetI ASST 384 380 . . 381 384 + CviJI RGCY 382 382 . . 381 384 + AluI AGCT 382 382 . . 382 392 + MwoI GCNNNNNNNGC 388 385 . . 382 386 + BlsI GCNGC 384 383 . . 382 386 + BisI GCNGC 383 384 . . 382 386 + TseI GCWGC 382 385 . . 384 387 + CviRI* TGCA 385 385 . . 382 386 - BbvI GCAGC 369 373 . . 392 396 + DdeI CTNAG 392 395 . . 402 405 + FatI CATG 401 405 . . 402 405 + NlaIII CATG 405 401 . . 402 405 + CviAII CATG 402 404 . . 415 419 + BlsI GCNGC 417 416 . . 415 419 + BisI GCNGC 416 417 . . 415 419 + TseI GCWGC 415 418 . . 412 416 - SfaNI GCATC 402 406 . . 415 419 - BbvI GCAGC 402 406 . . 416 421 - EcoP15I CAGCAG 388 390 . . 422 425 + TspEI AATT 421 425 . . 429 433 + MboII GAAGA 441 440 . . 448 458 + MwoI GCNNNNNNNGC 454 451 . . 449 454 + StyI CCWWGG 449 453 . . 449 454 + SecI* CCNNGG 449 453 . . 476 480 + TspDTI ATGAA 491 489 . . 489 493 - Hin4II* CCTTC 483 482 . . 516 519 + CviJI RGCY 517 517 . . 517 521 + BlsI GCNGC 519 518 . . 517 521 + BisI GCNGC 518 519 . . 517 521 + TseI GCWGC 517 520 . . 519 522 + CviRI* TGCA 520 520 . . 520 524 + SfaNI GCATC 529 533 . . 517 521 - BbvI GCAGC 504 508 . . 521 525 - FokI GGATG 507 511 . . 521 525 - BseGI GGATG 520 518 . . 538 541 + CviRI* TGCA 539 539 . . 543 546 + TaiI ACGT 546 542 . . 543 546 + SetI ASST 546 542 . . 543 546 + MaeII ACGT 543 545 . . 547 557 + MwoI GCNNNNNNNGC 553 550 . . 542 547 - BtrI CACGTC 544 544 . . 548 553 + Eco57MI CTGRAG 569 567 . . 548 553 + Eco57I CTGAAG 569 567 . . 565 570 + AhaIII* TTTAAA 567 567 . . 566 569 + MseI TTAA 566 568 . . 576 580 - BsrI ACTGG 576 574 . . 582 587 + VspI ATTAAT 583 585 . . 583 586 + MseI TTAA 583 585 . . 589 592 + FatI CATG 588 592 . . 589 592 + NlaIII CATG 592 588 . . 589 592 + CviAII CATG 589 591 . . 618 623 + AhaIII* TTTAAA 620 620 . . 619 622 + MseI TTAA 619 621 . . 628 632 + TspDTI ATGAA 643 641 . . 630 634 + MboII GAAGA 642 641 . . 640 643 + MseI TTAA 640 642 . . 644 647 - MnlI CCTC 637 636 . . 655 659 + MboII GAAGA 667 666 . . 658 662 - BccI CCATC 652 653 . . 665 670 + Hpy178III* TCNNGA 666 668 . . 669 673 + MboII GAAGA 681 680 . . 695 700 + Hpy166II GTNNAC 697 697 . . 695 700 + SduI GDGCHC 699 695 . . 695 700 + BseSI GKGCMC 699 695 . . 695 700 + ApaLI GTGCAC 695 699 . . 695 700 + HgiAI* GWGCWC 699 695 . . 696 699 + CviRI* TGCA 697 697 . . 704 707 + SetI ASST 707 703 . . 718 723 + SecI* CCNNGG 718 722 . . 719 723 + BssKI CCNGG 718 723 . . 719 723 + BseBI CCWGG 720 721 . . 719 723 + ScrFI CCNGG 720 721 . . 719 723 + EcoRII CCWGG 718 723 . . 721 724 + SetI ASST 724 720 . . 732 736 + SfaNI GCATC 741 745 . . 739 742 + CviJI RGCY 740 740 . . 749 752 + CviRI* TGCA 750 750 . . 747 751 - SfaNI GCATC 737 741 . . 754 758 + Tsp45I GTSAC 753 758 . . 754 758 + MaeIII GTNAC 753 758 . . 764 769 - EcoP15I CAGCAG 736 738 . . 776 779 + AciI CCGC 776 778 . . 795 798 + DpnI GATC 796 796 . . 795 798 + BstKTI GATC 797 795 . . 795 798 + MboI GATC 794 798 . . 804 807 + TspEI AATT 803 807 . . 807 810 + CviRI* TGCA 808 808 . . 810 813 + SetI ASST 813 809 . . 808 813 - BspMI ACCTGC 799 803 . . 842 846 + BccI CCATC 850 851 . . 855 859 - FokI GGATG 841 845 . . 855 859 - BseGI GGATG 854 852 . . 867 871 + Hpy188I TCNGA 869 868 . . 869 874 + XhoII RGATCY 869 873 . . 870 873 + DpnI GATC 871 871 . . 870 873 + BstKTI GATC 872 870 . . 870 873 + MboI GATC 869 873 . . 870 874 - BinI* GGATC 864 865 . . 881 884 + AciI CCGC 881 883 . . 883 887 + BlsI GCNGC 885 884 . . 883 887 + BisI GCNGC 884 885 . . 883 887 + BbvI GCAGC 895 899 . . 883 887 + TseI GCWGC 883 886 . . 894 898 + BmgT120I GGNCC 895 896 . . 894 898 + AsuI* GGNCC 894 897 . . 895 898 + CviJI RGCY 896 896 . . 895 898 + HaeIII GGCC 896 896 . . 901 904 + SetI ASST 904 900 . . 905 908 + CviRI* TGCA 906 906 . . 910 914 + MaeIII GTNAC 909 914 . . 914 919 + Eco57MI CTGRAG 935 933 . . 914 919 + Eco57I CTGAAG 935 933 . . 918 921 + SetI ASST 921 917 . . 919 924 + KpnI GGTACC 923 919 . . 919 924 + NlaIV GGNNCC 921 921 . . 919 924 + HgiCI* GGYRCC 919 923 . . 919 924 + Acc65I GGTACC 919 923 . . 916 920 - Hin4II* CCTTC 910 909 . . 920 923 + Csp6I GTAC 920 922 . . 920 923 + RsaI GTAC 921 921 . . 923 927 + BccI CCATC 931 932 . . 935 939 + MaeIII GTNAC 934 939 . . 933 938 - TsoI TARCCA 923 921 . . 959 963 - BsmAI GTCTC 953 957 . . 964 969 + HindIII AAGCTT 964 968 . . 965 968 + SetI ASST 968 964 . . 965 968 + CviJI RGCY 966 966 . . 965 968 + AluI AGCT 966 966 . . 972 977 + XhoII RGATCY 972 976 . . 972 977 + NlaIV GGNNCC 974 974 . . 972 977 + BamHI GGATCC 972 976 . . 972 976 + BinI* GGATC 980 981 . . 973 976 + DpnI GATC 974 974 . . 973 976 + BstKTI GATC 975 973 . . 973 976 + MboI GATC 972 976 . . 976 979 + AciI CCGC 976 978 . . 973 977 - BinI* GGATC 967 968 . . 978 982 + BlsI GCNGC 980 979 . . 978 982 + BisI GCNGC 979 980 . . 978 982 + BbvI GCAGC 990 994 . . 978 982 + TseI GCWGC 978 981 . . 978 989 + AlfI GCANNNNNNTGC 967 965 1001 999 980 983 + SetI ASST 983 979 . . 980 983 + CviJI RGCY 981 981 . . 980 983 + AluI AGCT 981 981 . . 978 989 - AlfI GCANNNNNNTGC 1001 999 967 965 1018 1022 + BssKI CCNGG 1017 1022 . . 1018 1021 + HpaII CCGG 1018 1020 . . 1018 1022 + ScrFI CCNGG 1019 1020 . . 1018 1022 + CauII* CCSGG 1019 1020 . . #--------------------------------------- # # # Acc65I: # [GGTACC] # 119 919 # # AccI: # [GTMKAC] # 86 952 # # AciI: # [CCGC] # 62 95 105 776 # # AclI: # [AACGTT] # 193 845 # # AflIII: # [ACRYGT] # 67 971 # # AhaIII*: # [TTTAAA] # 53 418 567 # # AlfI: # [GCANNNNNNTGC] # 0 0 34 37 967 # # AluI: # [AGCT] # 15 57 382 584 # # ApaLI: # [GTGCAC] # 343 695 # # AsuI*: # [GGNCC] # 43 144 851 # # AvaII: # [GGWCC] # 43 995 # # BamHI: # [GGATCC] # 66 972 # # BbvI: # [GCAGC] # 33 48 95 102 369 391 # # BccI: # [CCATC] # 81 92 107 198 256 304 # # BclI: # [TGATCA] # 207 831 # # BetI*: # [WCCGGW] # 72 966 # # BinI*: # [GGATC] # 13 58 103 292 572 # # BisI: # [GCNGC] # 33 59 95 102 366 383 # # BlsI: # [GCNGC] # 33 58 95 102 366 384 # # BmgT120I: # [GGNCC] # 44 143 851 # # BseBI: # [CCWGG] # 177 318 543 # # BseGI: # [GGATG] # 129 184 334 391 # # BseSI: # [GKGCMC] # 339 699 # # BsmAI: # [GTCTC] # 85 953 # # BspMI: # [ACCTGC] # 239 799 # # BspMII*: # [TCCGGA] # 72 966 # # BsrI: # [ACTGG] # 170 406 462 # # BssKI: # [CCNGG] # 21 175 299 543 # # BstKTI: # [GATC] # 63 75 77 103 210 510 # # BtrI: # [CACGTC] # 68 476 494 # # CauII*: # [CCSGG] # 19 1019 # # Csp6I: # [GTAC] # 118 151 225 544 # # CviAII: # [CATG] # 187 402 449 # # CviJI: # [RGCY] # 15 57 70 135 138 156 # 223 244 # # CviRI*: # [TGCA] # 19 53 58 80 98 132 # 135 158 305 # # DdeI: # [CTNAG] # 392 646 # # DpnI: # [GATC] # 64 75 77 103 209 510 # # DraII: # [RGGNCCY] # 43 995 # # DsaI*: # [CCRYGG] # 302 736 # # Eam1105I: # [GACNNNNNGTC] # 177 861 # # Eco57I: # [CTGAAG] # 103 366 569 # # Eco57MI: # [CTGRAG] # 103 366 569 # # EcoP15I: # [CAGCAG] # 302 348 388 # # EcoRII: # [CCWGG] # 175 320 543 # # FatI: # [CATG] # 187 401 450 # # FokI: # [GGATG] # 136 197 334 371 # # HaeIII: # [GGCC] # 142 896 # # HgiAI*: # [GWGCWC] # 138 339 561 # # HgiCI*: # [GGYRCC] # 119 919 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 163 843 # # Hin4II*: # [CCTTC] # 32 80 128 371 427 # # HindII: # [GTYRAC] # 171 867 # # HindIII: # [AAGCTT] # 74 964 # # HinfI: # [GANTC] # 314 724 # # HpaII: # [CCGG] # 20 73 945 # # HphI: # [GGTGA] # 2 21 57 229 729 # # Hpy166II: # [GTNNAC] # 84 87 341 526 # # Hpy178III*: # [TCNNGA] # 73 372 593 # # Hpy188I: # [TCNGA] # 24 53 106 106 169 580 # # KpnI: # [GGTACC] # 115 923 # # MaeI: # [CTAG] # 8 140 890 # # MaeII: # [ACGT] # 67 126 350 495 # # MaeIII: # [GTNAC] # 25 104 156 753 # # MboI: # [GATC] # 66 75 77 103 207 510 # # MboII: # [GAAGA] # 14 25 47 201 357 394 # # MmeI: # [TCCRAC] # 161 877 # # MnlI: # [CCTC] # 29 234 374 401 # # MseI: # [TTAA] # 17 21 36 398 566 # # MslI: # [CAYNNNNRTG] # 92 215 731 # # MwoI: # [GCNNNNNNNGC] # 66 99 388 485 # # NlaIII: # [CATG] # 187 405 446 # # NlaIV: # [GGNNCC] # 44 53 64 877 # # OliI: # [CACNNNNGTG] # 215 823 # # PpuMI: # [RGGWCCY] # 43 995 # # PshAI: # [GACNNNNGTC] # 227 811 # # RsaI: # [GTAC] # 117 152 225 544 # # ScaI: # [AGTACT] # 152 886 # # ScrFI: # [CCNGG] # 19 177 299 543 # # SduI: # [GDGCHC] # 138 339 561 # # SecI*: # [CCNNGG] # 147 269 302 320 # # SetI: # [ASST] # 15 15 17 17 47 55 # 70 79 89 91 109 111 # 161 162 # # SexAI: # [ACCWGGT] # 175 863 # # SfaNI: # [GCATC] # 4 127 208 297 402 # # SspI: # [AATATT] # 57 981 # # StyI: # [CCWWGG] # 449 589 # # TaiI: # [ACGT] # 70 126 350 492 # # TatI: # [WGTACW] # 150 225 663 # # TfiI: # [GAWTC] # 314 724 # # TseI: # [GCWGC] # 33 60 95 102 366 382 # # TsoI: # [TARCCA] # 115 923 # # Tsp45I: # [GTSAC] # 285 753 # # Tsp4CI*: # [ACNGT] # 217 821 # # TspDTI: # [ATGAA] # 105 152 386 395 # # TspEI: # [AATT] # 235 382 421 # # TspGWI: # [ACGGA] # 319 719 # # TstI: # [CACNNNNNNTCC] # 32 335 671 # # VspI: # [ATTAAT] # 455 583 # # XhoII: # [RGATCY] # 66 103 869 # # XmnI: # [GAANNNNTTC] # 195 843 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1038 # Reported_sequences: 1 # Reported_hitcount: 237 #---------------------------------------