Restriction Fragments for ROX1/YPR065W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

ROX1/YPR065W on chromosome XVI from coordinates 679693 to 680799.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.1805.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 1 5 + TspDTI ATGAA 16 14 . . 3 7 + TfiI GAWTC 3 6 . . 3 7 + HinfI GANTC 3 6 . . 14 17 + MnlI CCTC 24 23 . . 21 24 + SetI ASST 24 20 . . 23 27 + DdeI CTNAG 23 26 . . 27 31 + TfiI GAWTC 27 30 . . 27 31 + HinfI GANTC 27 30 . . 59 63 + Hpy188I TCNGA 61 60 . . 77 82 + XhoII RGATCY 77 81 . . 77 81 + BinI* GGATC 85 86 . . 78 81 + DpnI GATC 79 79 . . 78 81 + BstKTI GATC 80 78 . . 78 81 + MboI GATC 77 81 . . 82 85 + MseI TTAA 82 84 . . 95 99 + BmgT120I GGNCC 96 97 . . 95 99 + AvaII GGWCC 95 98 . . 95 99 + AsuI* GGNCC 95 98 . . 98 101 + AciI CCGC 98 100 . . 98 103 + BsrBI CCGCTC 100 100 . . 101 106 + SmlI CTYRAG 101 105 . . 105 108 + SetI ASST 108 104 . . 101 106 - Bce83I* CTTGAG 86 84 . . 124 127 + TspEI AATT 123 127 . . 141 144 + TspEI AATT 140 144 . . 149 152 + Csp6I GTAC 149 151 . . 149 152 + RsaI GTAC 150 150 . . 159 163 - Hin4II* CCTTC 153 152 . . 163 166 + CviJI RGCY 164 164 . . 171 176 + BetI* WCCGGW 171 175 . . 172 175 + HpaII CCGG 172 174 . . 175 179 + MboII GAAGA 187 186 . . 187 193 + TspRI CASTGNN 193 184 . . 188 192 + BsrI ACTGG 193 191 . . 188 193 + BfiI ACTGGG 198 197 . . 199 202 + MaeI CTAG 199 201 . . 202 205 - AciI CCGC 202 204 . . 207 211 - Hin4II* CCTTC 201 200 . . 217 220 + MaeI CTAG 217 219 . . 223 226 + FatI CATG 222 226 . . 223 226 + NlaIII CATG 226 222 . . 223 226 + CviAII CATG 223 225 . . 224 228 + TspDTI ATGAA 239 237 . . 234 239 + BciVI GTATCC 245 244 . . 237 242 + Hpy178III* TCNNGA 238 240 . . 254 257 + CviJI RGCY 255 255 . . 255 260 + Cfr10I RCCGGY 255 259 . . 256 259 + HpaII CCGG 256 258 . . 269 273 + DdeI CTNAG 269 272 . . 273 277 + MboII GAAGA 285 284 . . 291 295 - Hin4II* CCTTC 285 284 . . 299 302 + TaqI TCGA 299 301 . . 303 313 + MwoI GCNNNNNNNGC 309 306 . . 307 312 + EcoP15I CAGCAG 337 339 . . 309 313 + BlsI GCNGC 311 310 . . 309 313 + BisI GCNGC 310 311 . . 309 313 + BbvI GCAGC 321 325 . . 309 313 + TseI GCWGC 309 312 . . 310 315 + EcoP15I CAGCAG 340 342 . . 312 316 + BlsI GCNGC 314 313 . . 312 316 + BisI GCNGC 313 314 . . 312 316 + BbvI GCAGC 324 328 . . 312 316 + TseI GCWGC 312 315 . . 331 336 + EcoP15I CAGCAG 361 363 . . 333 337 + BlsI GCNGC 335 334 . . 333 337 + BisI GCNGC 334 335 . . 333 337 + BbvI GCAGC 345 349 . . 333 337 + TseI GCWGC 333 336 . . 334 339 + EcoP15I CAGCAG 364 366 . . 336 340 + BlsI GCNGC 338 337 . . 336 340 + BisI GCNGC 337 338 . . 336 340 + BbvI GCAGC 348 352 . . 336 340 + TseI GCWGC 336 339 . . 337 342 + EcoP15I CAGCAG 367 369 . . 345 349 + Tsp4CI* ACNGT 347 346 . . 348 352 + Tsp45I GTSAC 347 352 . . 348 352 + MaeIII GTNAC 347 352 . . 355 358 + AciI CCGC 355 357 . . 359 362 + TspEI AATT 358 362 . . 368 371 + CviJI RGCY 369 369 . . 374 377 + MseI TTAA 374 376 . . 394 398 + TspDTI ATGAA 409 407 . . 401 406 + SduI GDGCHC 405 401 . . 401 406 + HgiAI* GWGCWC 405 401 . . 418 422 + BccI CCATC 426 427 . . 415 419 - HphI GGTGA 407 406 . . 425 428 + MnlI CCTC 435 434 . . 421 425 - MboII GAAGA 413 412 . . 425 430 + SecI* CCNNGG 425 429 . . 436 439 + SetI ASST 439 435 . . 436 439 + CviJI RGCY 437 437 . . 436 439 + AluI AGCT 437 437 . . 437 447 + MwoI GCNNNNNNNGC 443 440 . . 439 442 + TaqI TCGA 439 441 . . 445 448 + SetI ASST 448 444 . . 445 448 + CviJI RGCY 446 446 . . 445 448 + AluI AGCT 446 446 . . 457 462 + MfeI CAATTG 457 461 . . 458 461 + TspEI AATT 457 461 . . 469 472 + DpnI GATC 470 470 . . 469 472 + BstKTI GATC 471 469 . . 469 472 + MboI GATC 468 472 . . 472 477 + SmlI CTYRAG 472 476 . . 472 477 + AflII CTTAAG 472 476 . . 473 476 + MseI TTAA 473 475 . . 478 481 + SetI ASST 481 477 . . 477 480 - MnlI CCTC 470 469 . . 490 494 + Hin4II* CCTTC 500 499 . . 497 500 + MseI TTAA 497 499 . . 516 521 + Eco31I GGTCTC 522 526 . . 517 521 + BsmAI GTCTC 522 526 . . 520 525 + Hpy178III* TCNNGA 521 523 . . 523 528 + XhoII RGATCY 523 527 . . 523 528 + BglII AGATCT 523 527 . . 519 524 - Eco57MI CTGRAG 504 502 . . 524 527 + DpnI GATC 525 525 . . 524 527 + BstKTI GATC 526 524 . . 519 524 - GsuI CTGGAG 504 502 . . 524 527 + MboI GATC 523 527 . . 529 532 + MseI TTAA 529 531 . . 533 538 + Hpy166II GTNNAC 535 535 . . 540 543 + SetI ASST 543 539 . . 546 550 + HgaI GACGC 555 560 . . 550 553 + FatI CATG 549 553 . . 550 553 + NlaIII CATG 553 549 . . 550 553 + CviAII CATG 550 552 . . 559 563 + BceAI ACGGC 575 577 . . 569 572 + SetI ASST 572 568 . . 566 570 - BsmAI GTCTC 560 564 . . 566 571 - Eco31I GGTCTC 560 564 . . 577 582 + PvuII CAGCTG 579 579 . . 577 582 + NspBII* CMGCKG 579 579 . . 578 581 + SetI ASST 581 577 . . 578 581 + CviJI RGCY 579 579 . . 578 581 + AluI AGCT 579 579 . . 594 599 + ApoI RAATTY 594 598 . . 595 598 + TspEI AATT 594 598 . . 612 616 + DdeI CTNAG 612 615 . . 612 616 + BspCNI CTCAG 625 623 . . 612 616 + BseMII CTCAG 626 624 . . 615 618 + SetI ASST 618 614 . . 615 618 + CviJI RGCY 616 616 . . 615 618 + AluI AGCT 616 616 . . 628 632 + Hin4II* CCTTC 638 637 . . 636 640 - BsmAI GTCTC 630 634 . . 647 650 + SetI ASST 650 646 . . 648 651 + MnlI CCTC 658 657 . . 653 656 + TaiI ACGT 656 652 . . 653 656 + SetI ASST 656 652 . . 653 656 + MaeII ACGT 653 655 . . 654 658 + Hpy99I CGWCG 658 653 . . 659 664 + SmlI CTYRAG 659 663 . . 663 666 + SetI ASST 666 662 . . 663 666 + CviJI RGCY 664 664 . . 663 666 + AluI AGCT 664 664 . . 659 664 - Bce83I* CTTGAG 644 642 . . 670 675 + StyI CCWWGG 670 674 . . 670 675 + SecI* CCNNGG 670 674 . . 675 678 + CviJI RGCY 676 676 . . 682 685 + TaqI TCGA 682 684 . . 688 691 + MnlI CCTC 698 697 . . 691 696 + MfeI CAATTG 691 695 . . 692 695 + TspEI AATT 691 695 . . 705 709 - TspDTI ATGAA 695 693 . . 707 711 - FokI GGATG 693 697 . . 707 711 - BseGI GGATG 706 704 . . 723 728 + EcoP15I CAGCAG 753 755 . . 735 740 + Cfr10I RCCGGY 735 739 . . 735 740 + BetI* WCCGGW 735 739 . . 735 740 + AgeI ACCGGT 735 739 . . 736 739 + HpaII CCGG 736 738 . . 739 743 + MaeIII GTNAC 738 743 . . 755 759 - FokI GGATG 741 745 . . 755 759 - BseGI GGATG 754 752 . . 771 781 + Hin4I GAYNNNNNVTC 762 757 794 789 772 782 + BsaXI ACNNNNNCTCC 762 759 792 789 775 779 - MboII GAAGA 767 766 . . 781 785 + BssKI CCNGG 780 785 . . 781 785 + BseBI CCWGG 782 783 . . 781 786 + SecI* CCNNGG 781 785 . . 781 785 + ScrFI CCNGG 782 783 . . 781 785 + EcoRII CCWGG 780 785 . . 787 792 + ApoI RAATTY 787 791 . . 788 791 + TspEI AATT 787 791 . . 794 797 + MnlI CCTC 804 803 . . 795 800 + Ksp632I* CTCTTC 801 804 . . 792 797 - BseRI GAGGAG 783 781 . . 796 800 - MboII GAAGA 788 787 . . 801 805 + Hpy188I TCNGA 803 802 . . 809 812 + MnlI CCTC 819 818 . . 807 812 - BseRI GAGGAG 798 796 . . 813 818 + TatI WGTACW 813 817 . . 814 817 + Csp6I GTAC 814 816 . . 814 817 + RsaI GTAC 815 815 . . 816 820 + BsrI ACTGG 821 819 . . 817 822 + Eco57MI CTGRAG 838 836 . . 817 822 + GsuI CTGGAG 838 836 . . 832 835 + MseI TTAA 832 834 . . 836 840 + Tsp4CI* ACNGT 838 837 . . 847 850 + TspEI AATT 846 850 . . 857 862 + SspI AATATT 859 859 . . 864 867 + SetI ASST 867 863 . . 865 868 + MnlI CCTC 875 874 . . 871 874 + MnlI CCTC 881 880 . . 879 882 + SetI ASST 882 878 . . 880 884 + Hin4II* CCTTC 890 889 . . 887 890 + CviRI* TGCA 888 888 . . 900 904 + BsrI ACTGG 905 903 . . 903 907 + BinI* GGATC 911 912 . . 904 907 + DpnI GATC 905 905 . . 904 907 + BstKTI GATC 906 904 . . 904 907 + MboI GATC 903 907 . . 909 912 + Csp6I GTAC 909 911 . . 909 912 + RsaI GTAC 910 910 . . 913 918 + EcoP15I CAGCAG 943 945 . . 915 925 + MwoI GCNNNNNNNGC 921 918 . . 915 919 + BlsI GCNGC 917 916 . . 915 919 + BisI GCNGC 916 917 . . 915 919 + BbvI GCAGC 927 931 . . 915 919 + TseI GCWGC 915 918 . . 917 920 + SetI ASST 920 916 . . 917 920 + CviJI RGCY 918 918 . . 917 920 + AluI AGCT 918 918 . . 930 934 + BslFI GGGAC 944 948 . . 930 935 + NlaIV GGNNCC 932 932 . . 931 935 + BmgT120I GGNCC 932 933 . . 927 931 - BccI CCATC 921 922 . . 931 935 + AvaII GGWCC 931 934 . . 931 935 + AsuI* GGNCC 931 934 . . 953 959 + TspRI CASTGNN 959 950 . . 954 958 + Tsp4CI* ACNGT 956 955 . . 957 961 + BsmAI GTCTC 962 966 . . 965 969 + BssKI CCNGG 964 969 . . 965 969 + BseBI CCWGG 966 967 . . 965 969 + ScrFI CCNGG 966 967 . . 965 969 + EcoRII CCWGG 964 969 . . 981 986 + Hpy166II GTNNAC 983 983 . . 999 1003 + SfaNI GCATC 1008 1012 . . 1012 1015 + MnlI CCTC 1022 1021 . . 1036 1039 + MnlI CCTC 1046 1045 . . 1047 1050 + TspEI AATT 1046 1050 . . 1049 1054 + PsiI TTATAA 1051 1051 . . 1056 1061 + SmlI CTYRAG 1056 1060 . . 1056 1061 - Bce83I* CTTGAG 1041 1039 . . 1065 1071 + TspRI CASTGNN 1071 1062 . . 1067 1071 + DdeI CTNAG 1067 1070 . . 1070 1073 + SetI ASST 1073 1069 . . 1071 1077 + BstEII GGTNACC 1071 1076 . . 1067 1071 - BspCNI CTCAG 1059 1057 . . 1067 1071 - BseMII CTCAG 1058 1056 . . 1072 1076 + Tsp45I GTSAC 1071 1076 . . 1069 1072 - MnlI CCTC 1062 1061 . . 1072 1076 + MaeIII GTNAC 1071 1076 . . 1076 1079 + AciI CCGC 1076 1078 . . 1073 1077 - HphI GGTGA 1065 1064 . . 1085 1088 + MaeI CTAG 1085 1087 . . 1087 1090 + CviJI RGCY 1088 1088 . . 1090 1093 + MaeI CTAG 1090 1092 . . 1098 1102 + Hpy188I TCNGA 1100 1099 . . #--------------------------------------- # # # AciI: # [CCGC] # 31 98 104 153 721 # # AflII: # [CTTAAG] # 472 635 # # AgeI: # [ACCGGT] # 372 735 # # AluI: # [AGCT] # 9 37 48 133 189 254 # 437 # # ApoI: # [RAATTY] # 193 320 594 # # AsuI*: # [GGNCC] # 95 176 836 # # AvaII: # [GGWCC] # 95 176 836 # # BbvI: # [GCAGC] # 3 3 21 180 321 579 # # BccI: # [CCATC] # 186 426 495 # # Bce83I*: # [CTTGAG] # 66 86 397 558 # # BceAI: # [ACGGC] # 532 575 # # BciVI: # [GTATCC] # 245 862 # # BetI*: # [WCCGGW] # 171 372 564 # # BfiI: # [ACTGGG] # 198 909 # # BglII: # [AGATCT] # 523 584 # # BinI*: # [GGATC] # 85 196 826 # # BisI: # [GCNGC] # 3 3 21 191 310 579 # # BlsI: # [GCNGC] # 3 3 21 190 311 579 # # BmgT120I: # [GGNCC] # 96 175 836 # # BsaXI: # [ACNNNNNCTCC] # 30 315 762 # # BseBI: # [CCWGG] # 141 184 782 # # BseGI: # [GGATG] # 48 353 706 # # BseMII: # [CTCAG] # 49 432 626 # # BseRI: # [GAGGAG] # 15 309 783 # # BslFI: # [GGGAC] # 163 944 # # BsmAI: # [GTCTC] # 38 70 145 332 522 # # BspCNI: # [CTCAG] # 48 434 625 # # BsrBI: # [CCGCTC] # 100 1007 # # BsrI: # [ACTGG] # 84 193 202 628 # # BssKI: # [CCNGG] # 143 184 780 # # BstEII: # [GGTNACC] # 36 1071 # # BstKTI: # [GATC] # 55 80 201 380 391 # # Cfr10I: # [RCCGGY] # 255 372 480 # # Csp6I: # [GTAC] # 95 149 198 665 # # CviAII: # [CATG] # 223 327 557 # # CviJI: # [RGCY] # 9 12 19 37 48 68 # 91 114 133 164 170 242 # # CviRI*: # [TGCA] # 219 888 # # DdeI: # [CTNAG] # 23 40 246 343 455 # # DpnI: # [GATC] # 55 79 202 380 391 # # Eco31I: # [GGTCTC] # 38 522 547 # # Eco57MI: # [CTGRAG] # 269 334 504 # # EcoP15I: # [CAGCAG] # 3 3 3 21 164 190 # 337 386 # # EcoRII: # [CCWGG] # 143 184 780 # # FatI: # [CATG] # 222 327 558 # # FokI: # [GGATG] # 48 366 693 # # GsuI: # [CTGGAG] # 269 334 504 # # HgaI: # [GACGC] # 552 555 # # HgiAI*: # [GWGCWC] # 405 702 # # Hin4I: # [GAYNNNNNVTC] # 32 313 762 # # Hin4II*: # [CCTTC] # 48 84 138 153 215 217 # 252 # # HinfI: # [GANTC] # 3 24 1080 # # HpaII: # [CCGG] # 84 172 371 480 # # HphI: # [GGTGA] # 42 407 658 # # Hpy166II: # [GTNNAC] # 124 448 535 # # Hpy178III*: # [TCNNGA] # 238 283 586 # # Hpy188I: # [TCNGA] # 7 61 297 742 # # Hpy99I: # [CGWCG] # 449 658 # # Ksp632I*: # [CTCTTC] # 306 801 # # MaeI: # [CTAG] # 5 17 18 199 868 # # MaeII: # [ACGT] # 454 653 # # MaeIII: # [GTNAC] # 36 333 347 391 # # MboI: # [GATC] # 55 77 204 380 391 # # MboII: # [GAAGA] # 21 98 128 187 319 354 # # MfeI: # [CAATTG] # 234 416 457 # # MnlI: # [CCTC] # 6 15 16 24 24 35 # 40 45 56 106 141 188 # 411 # # MseI: # [TTAA] # 24 32 82 99 275 292 # 303 # # MwoI: # [GCNNNNNNNGC] # 134 186 309 478 # # NlaIII: # [CATG] # 226 327 554 # # NlaIV: # [GGNNCC] # 175 932 # # NspBII*: # [CMGCKG] # 528 579 # # PsiI: # [TTATAA] # 56 1051 # # PvuII: # [CAGCTG] # 528 579 # # RsaI: # [GTAC] # 95 150 197 665 # # ScrFI: # [CCNGG] # 141 184 782 # # SduI: # [GDGCHC] # 405 702 # # SecI*: # [CCNNGG] # 111 245 326 425 # # SetI: # [ASST] # 6 9 9 10 15 24 # 29 32 33 34 37 38 # 62 84 153 201 331 # # SfaNI: # [GCATC] # 99 1008 # # SmlI: # [CTYRAG] # 51 101 187 371 397 # # SspI: # [AATATT] # 248 859 # # StyI: # [CCWWGG] # 437 670 # # TaiI: # [ACGT] # 451 656 # # TaqI: # [TCGA] # 140 243 299 425 # # TatI: # [WGTACW] # 294 813 # # TfiI: # [GAWTC] # 3 24 1080 # # TseI: # [GCWGC] # 3 3 21 192 309 579 # # Tsp45I: # [GTSAC] # 36 347 724 # # Tsp4CI*: # [ACNGT] # 118 151 347 491 # # TspDTI: # [ATGAA] # 16 170 223 286 412 # # TspEI: # [AATT] # 17 59 61 96 97 99 # 123 137 200 218 # # TspRI: # [CASTGNN] # 36 112 193 766 # # XhoII: # [RGATCY] # 77 446 584 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1107 # Reported_sequences: 1 # Reported_hitcount: 259 #---------------------------------------