Restriction Fragments for KAR9/YPL269W



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The currently selected gene/sequence is :

KAR9/YPL269W on chromosome XVI from coordinates 33013 to 34947.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.10563.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 13 18 + NlaIV GGNNCC 15 15 . . 13 17 + BmgT120I GGNCC 14 15 . . 13 17 + AvaII GGWCC 13 16 . . 13 17 + AsuI* GGNCC 13 16 . . 10 14 - BccI CCATC 4 5 . . 19 24 + XhoII RGATCY 19 23 . . 19 24 + BglII AGATCT 19 23 . . 20 23 + DpnI GATC 21 21 . . 20 23 + BstKTI GATC 22 20 . . 20 23 + MboI GATC 19 23 . . 36 40 + FokI GGATG 49 53 . . 36 40 + BseGI GGATG 42 40 . . 59 64 + Hpy166II GTNNAC 61 61 . . 62 67 + AclI AACGTT 63 65 . . 63 66 + TaiI ACGT 66 62 . . 63 66 + SetI ASST 66 62 . . 63 66 + MaeII ACGT 63 65 . . 70 79 + XmnI GAANNNNTTC 74 74 . . 74 79 + EcoRI GAATTC 74 78 . . 74 79 + ApoI RAATTY 74 78 . . 75 78 + TspEI AATT 74 78 . . 103 113 + Hin4I GAYNNNNNVTC 94 89 126 121 106 109 + CviRI* TGCA 107 107 . . 125 128 + Csp6I GTAC 125 127 . . 125 128 + RsaI GTAC 126 126 . . 139 143 + Hpy188I TCNGA 141 140 . . 142 146 - BsmAI GTCTC 136 140 . . 158 161 + TspEI AATT 157 161 . . 177 188 + PpiI GAACNNNNNCTC 169 164 201 196 178 183 + AclI AACGTT 179 181 . . 179 182 + TaiI ACGT 182 178 . . 179 182 + SetI ASST 182 178 . . 179 182 + MaeII ACGT 179 181 . . 185 188 + MnlI CCTC 195 194 . . 185 190 + SecI* CCNNGG 185 189 . . 192 195 + TspEI AATT 191 195 . . 195 200 + Hpy178III* TCNNGA 196 198 . . 199 207 + Tth111I GACNNNGTC 202 203 . . 201 205 + DdeI CTNAG 201 204 . . 205 210 + Hpy166II GTNNAC 207 207 . . 205 210 + HindII GTYRAC 207 207 . . 219 223 + BsrI ACTGG 224 222 . . 219 224 + BfiI ACTGGG 229 228 . . 251 255 + BsrI ACTGG 256 254 . . 253 258 - TsoI TARCCA 243 241 . . 284 287 + TspEI AATT 283 287 . . 293 297 + Tsp45I GTSAC 292 297 . . 293 297 + MaeIII GTNAC 292 297 . . 296 299 + TaiI ACGT 299 295 . . 296 299 + SetI ASST 299 295 . . 296 299 + MaeII ACGT 296 298 . . 299 303 - TspDTI ATGAA 289 287 . . 304 309 + SspI AATATT 306 306 . . 310 313 - MnlI CCTC 303 302 . . 315 318 + SetI ASST 318 314 . . 313 317 - Hin4II* CCTTC 307 306 . . 330 335 - MmeI TCCRAC 311 309 . . 340 343 + MaeI CTAG 340 342 . . 345 349 + FokI GGATG 358 362 . . 345 349 + BseGI GGATG 351 349 . . 343 346 - MnlI CCTC 336 335 . . 346 356 + Hin4I GAYNNNNNVTC 337 332 369 364 347 351 + TspDTI ATGAA 362 360 . . 349 354 + EcoRI GAATTC 349 353 . . 349 354 + ApoI RAATTY 349 353 . . 350 353 + TspEI AATT 349 353 . . 353 358 + Hpy178III* TCNNGA 354 356 . . 355 358 + TaqI TCGA 355 357 . . 357 361 - Hin4II* CCTTC 351 350 . . 364 367 + DpnI GATC 365 365 . . 364 367 + BstKTI GATC 366 364 . . 364 367 + MboI GATC 363 367 . . 366 371 + Hpy178III* TCNNGA 367 369 . . 386 391 + ClaI ATCGAT 387 389 . . 387 390 + TaqI TCGA 387 389 . . 394 397 + CviJI RGCY 395 395 . . 406 411 + ZraI GACGTC 408 408 . . 406 411 + AcyI GRCGYC 407 409 . . 406 411 + AatII GACGTC 410 406 . . 407 410 + TaiI ACGT 410 406 . . 407 410 + SetI ASST 410 406 . . 407 410 + MaeII ACGT 407 409 . . 415 419 + MboII GAAGA 427 426 . . 418 422 + TfiI GAWTC 418 421 . . 418 422 + HinfI GANTC 418 421 . . 429 432 + TspEI AATT 428 432 . . 431 434 + MseI TTAA 431 433 . . 435 439 + HinfI GANTC 435 438 . . 437 441 + DdeI CTNAG 437 440 . . 437 441 + BspCNI CTCAG 450 448 . . 437 441 + BseMII CTCAG 451 449 . . 435 439 - PleI GAGTC 429 430 . . 435 439 - MlyI GAGTC 429 429 . . 444 449 + NlaIV GGNNCC 446 446 . . 441 446 - MmeI TCCRAC 422 420 . . 446 449 + CviJI RGCY 447 447 . . 448 453 + DsaI* CCRYGG 448 452 . . 448 453 + StyI CCWWGG 448 452 . . 448 453 + NcoI CCATGG 448 452 . . 448 453 + SecI* CCNNGG 448 452 . . 449 452 + FatI CATG 448 452 . . 449 452 + NlaIII CATG 452 448 . . 449 452 + CviAII CATG 449 451 . . 452 455 + CviJI RGCY 453 453 . . 465 478 + AjuI GAANNNNNNNTTGG 457 452 489 484 465 469 - Hin4II* CCTTC 459 458 . . 470 473 + TspEI AATT 469 473 . . 481 484 + TaiI ACGT 484 480 . . 481 484 + SetI ASST 484 480 . . 481 484 + MaeII ACGT 481 483 . . 477 482 - BciVI GTATCC 471 470 . . 490 495 + ApoI RAATTY 490 494 . . 491 494 + TspEI AATT 490 494 . . 494 497 + MseI TTAA 494 496 . . 500 505 + ApoI RAATTY 500 504 . . 501 504 + TspEI AATT 500 504 . . 510 514 + BinI* GGATC 518 519 . . 511 514 + DpnI GATC 512 512 . . 511 514 + BstKTI GATC 513 511 . . 511 514 + MboI GATC 510 514 . . 514 519 + NdeI CATATG 515 517 . . 519 524 - BciVI GTATCC 513 512 . . 538 543 + VspI ATTAAT 539 541 . . 539 542 + MseI TTAA 539 541 . . 561 566 + Hpy178III* TCNNGA 562 564 . . 566 571 + ApoI RAATTY 566 570 . . 567 570 + TspEI AATT 566 570 . . 570 575 + Hpy178III* TCNNGA 571 573 . . 574 578 + MboII GAAGA 586 585 . . 580 583 + SetI ASST 583 579 . . 573 585 - AloI GAACNNNNNNTCC 597 592 565 560 585 588 + FnuDII* CGCG 586 586 . . 586 590 - HgaI GACGC 575 580 . . 590 600 + BsiYI* CCNNNNNNNGG 596 593 . . 588 592 - BslFI GGGAC 573 577 . . 597 601 + BceAI ACGGC 613 615 . . 607 611 + BccI CCATC 615 616 . . 619 622 + MaeI CTAG 619 621 . . 627 632 + SpeI ACTAGT 627 631 . . 628 631 + MaeI CTAG 628 630 . . 652 656 + TspGWI ACGGA 667 665 . . 655 659 - BsmAI GTCTC 649 653 . . 662 666 + DdeI CTNAG 662 665 . . 665 668 + CviJI RGCY 666 666 . . 662 666 - BspCNI CTCAG 654 652 . . 662 666 - BseMII CTCAG 653 651 . . 671 674 + SetI ASST 674 670 . . 672 677 + KpnI GGTACC 676 672 . . 672 677 + NlaIV GGNNCC 674 674 . . 672 677 + HgiCI* GGYRCC 672 676 . . 672 677 + Acc65I GGTACC 672 676 . . 673 676 + Csp6I GTAC 673 675 . . 673 676 + RsaI GTAC 674 674 . . 679 684 + ApoI RAATTY 679 683 . . 680 683 + TspEI AATT 679 683 . . 688 691 + HpaII CCGG 688 690 . . 694 698 + MboII GAAGA 706 705 . . 697 707 + Hin4I GAYNNNNNVTC 688 683 720 715 706 711 + Hpy178III* TCNNGA 707 709 . . 697 707 - Hin4I GAYNNNNNVTC 720 715 688 683 716 721 + Hpy178III* TCNNGA 717 719 . . 722 725 + SetI ASST 725 721 . . 739 742 + MnlI CCTC 749 748 . . 743 748 + MfeI CAATTG 743 747 . . 744 747 + TspEI AATT 743 747 . . 763 768 + EcoRV GATATC 765 765 . . 771 774 + SetI ASST 774 770 . . 772 775 + MnlI CCTC 782 781 . . 780 783 + TspEI AATT 779 783 . . 785 788 + CviRI* TGCA 786 786 . . 786 796 + MwoI GCNNNNNNNGC 792 789 . . 788 791 + TspEI AATT 787 791 . . 793 798 + SduI GDGCHC 797 793 . . 793 798 + BseSI GKGCMC 797 793 . . 794 799 + NlaIV GGNNCC 796 796 . . 794 799 + HgiCI* GGYRCC 794 798 . . 797 802 + BetI* WCCGGW 797 801 . . 798 801 + HpaII CCGG 798 800 . . 824 827 + CviRI* TGCA 825 825 . . 836 839 + CviRI* TGCA 837 837 . . 851 854 + SetI ASST 854 850 . . 851 854 + CviJI RGCY 852 852 . . 851 854 + AluI AGCT 852 852 . . 859 863 + TspDTI ATGAA 874 872 . . 877 881 + TspDTI ATGAA 892 890 . . 879 884 + EcoRI GAATTC 879 883 . . 879 884 + ApoI RAATTY 879 883 . . 880 883 + TspEI AATT 879 883 . . 883 887 + Hpy188I TCNGA 885 884 . . 896 899 + TspEI AATT 895 899 . . 905 908 + TaqI TCGA 905 907 . . 914 917 + TaqI TCGA 914 916 . . 920 925 + AclI AACGTT 921 923 . . 921 924 + TaiI ACGT 924 920 . . 921 924 + SetI ASST 924 920 . . 921 924 + MaeII ACGT 921 923 . . 923 928 - MmeI TCCRAC 904 902 . . 940 945 + VspI ATTAAT 941 943 . . 941 944 + MseI TTAA 941 943 . . 943 946 + TspEI AATT 942 946 . . 944 951 + SwaI ATTTAAAT 947 947 . . 945 950 + AhaIII* TTTAAA 947 947 . . 946 949 + MseI TTAA 946 948 . . 951 956 + Hpy178III* TCNNGA 952 954 . . 951 956 + BspHI TCATGA 951 955 . . 952 955 + FatI CATG 951 955 . . 952 955 + NlaIII CATG 955 951 . . 952 955 + CviAII CATG 952 954 . . 953 957 + TspDTI ATGAA 968 966 . . 969 974 + Hpy178III* TCNNGA 970 972 . . 985 988 + SetI ASST 988 984 . . 989 992 + CviRI* TGCA 990 990 . . 994 997 + TaqI TCGA 994 996 . . 998 1001 + TspEI AATT 997 1001 . . 1014 1019 + Hpy166II GTNNAC 1016 1016 . . 1014 1019 + BssNAI GTATAC 1016 1016 . . 1014 1019 + AccI GTMKAC 1015 1017 . . 1024 1029 + EcoRV GATATC 1026 1026 . . 1037 1040 + CviJI RGCY 1038 1038 . . 1038 1048 + MwoI GCNNNNNNNGC 1044 1041 . . 1039 1044 + SmlI CTYRAG 1039 1043 . . 1039 1044 + XhoI CTCGAG 1039 1043 . . 1039 1044 + BmeT110I CYCGRG 1040 1042 . . 1039 1044 + AvaI CYCGRG 1039 1043 . . 1040 1043 + TaqI TCGA 1040 1042 . . 1040 1045 + Hpy178III* TCNNGA 1041 1043 . . 1044 1047 - MnlI CCTC 1037 1036 . . 1048 1053 + MfeI CAATTG 1048 1052 . . 1049 1052 + TspEI AATT 1048 1052 . . 1054 1064 - Hin4I GAYNNNNNVTC 1077 1072 1045 1040 1069 1073 + Tsp4CI* ACNGT 1071 1070 . . 1076 1080 + DdeI CTNAG 1076 1079 . . 1087 1092 + SspI AATATT 1089 1089 . . 1108 1113 + EcoRI GAATTC 1108 1112 . . 1108 1113 + ApoI RAATTY 1108 1112 . . 1109 1112 + TspEI AATT 1108 1112 . . 1123 1127 + HgaI GACGC 1132 1137 . . 1123 1134 + DrdI GACNNNNNNGTC 1129 1127 . . 1126 1136 + MwoI GCNNNNNNNGC 1132 1129 . . 1126 1131 + Cac8I GCNNGC 1128 1128 . . 1129 1134 + AcyI GRCGYC 1130 1132 . . 1131 1135 + Hpy99I CGWCG 1135 1130 . . 1130 1134 - HgaI GACGC 1119 1124 . . 1135 1139 + SfaNI GCATC 1144 1148 . . 1138 1141 + TaqI TCGA 1138 1140 . . 1140 1144 + MboII GAAGA 1152 1151 . . 1140 1145 + BbvII* GAAGAC 1147 1151 . . 1148 1152 + TspDTI ATGAA 1163 1161 . . 1149 1160 + BdaI TGANNNNNNTCA 1138 1136 1172 1170 1151 1154 + TspEI AATT 1150 1154 . . 1155 1158 + SetI ASST 1158 1154 . . 1155 1158 + CviJI RGCY 1156 1156 . . 1155 1158 + AluI AGCT 1156 1156 . . 1149 1160 - BdaI TGANNNNNNTCA 1172 1170 1138 1136 1166 1169 + CviJI RGCY 1167 1167 . . 1163 1167 - BccI CCATC 1157 1158 . . 1178 1181 + CviJI RGCY 1179 1179 . . 1181 1186 + SfeI* CTRYAG 1181 1185 . . 1184 1189 + NspBII* CMGCKG 1186 1186 . . 1186 1189 - AciI CCGC 1186 1188 . . 1193 1198 + ApoI RAATTY 1193 1197 . . 1194 1197 + TspEI AATT 1193 1197 . . 1208 1211 + MnlI CCTC 1218 1217 . . 1211 1214 + AciI CCGC 1211 1213 . . 1227 1230 + SetI ASST 1230 1226 . . 1227 1230 + CviJI RGCY 1228 1228 . . 1227 1230 + AluI AGCT 1228 1228 . . 1232 1235 + MaeI CTAG 1232 1234 . . 1239 1243 + MboII GAAGA 1251 1250 . . 1242 1246 + MboII GAAGA 1254 1253 . . 1245 1249 + TfiI GAWTC 1245 1248 . . 1245 1249 + HinfI GANTC 1245 1248 . . 1267 1270 + CviJI RGCY 1268 1268 . . 1276 1281 + McrI* CGRYCG 1279 1277 . . 1277 1282 + TaqII GACCGA 1293 1291 . . 1304 1309 + SecI* CCNNGG 1304 1308 . . 1305 1309 + BssKI CCNGG 1304 1309 . . 1305 1309 + BseBI CCWGG 1306 1307 . . 1305 1309 + ScrFI CCNGG 1306 1307 . . 1305 1309 + EcoRII CCWGG 1304 1309 . . 1313 1319 + PfoI TCCNGGA 1313 1318 . . 1314 1318 + BssKI CCNGG 1313 1318 . . 1314 1318 + BseBI CCWGG 1315 1316 . . 1314 1318 + ScrFI CCNGG 1315 1316 . . 1314 1318 + EcoRII CCWGG 1313 1318 . . 1322 1326 + PleI GAGTC 1330 1331 . . 1322 1326 + MlyI GAGTC 1331 1331 . . 1322 1326 + HinfI GANTC 1322 1325 . . 1330 1335 + VspI ATTAAT 1331 1333 . . 1331 1334 + MseI TTAA 1331 1333 . . 1334 1338 + TspDTI ATGAA 1349 1347 . . 1338 1341 + SetI ASST 1341 1337 . . 1339 1343 + HphI GGTGA 1351 1350 . . 1340 1344 + Tsp45I GTSAC 1339 1344 . . 1340 1344 + MaeIII GTNAC 1339 1344 . . 1336 1340 - Hin4II* CCTTC 1330 1329 . . 1343 1347 + Tsp4CI* ACNGT 1345 1344 . . 1356 1361 + BetI* WCCGGW 1356 1360 . . 1357 1360 + HpaII CCGG 1357 1359 . . 1362 1367 + ApoI RAATTY 1362 1366 . . 1363 1366 + TspEI AATT 1362 1366 . . 1367 1378 + CspCI CAANNNNNGTGG 1355 1353 1390 1388 1370 1375 + SfeI* CTRYAG 1370 1374 . . 1372 1376 + Tsp4CI* ACNGT 1374 1373 . . 1373 1379 + TspRI CASTGNN 1379 1370 . . 1377 1380 + CviJI RGCY 1378 1378 . . 1386 1389 + SetI ASST 1389 1385 . . 1393 1398 + HindIII AAGCTT 1393 1397 . . 1394 1397 + SetI ASST 1397 1393 . . 1394 1397 + CviJI RGCY 1395 1395 . . 1394 1397 + AluI AGCT 1395 1395 . . 1407 1411 + MaeIII GTNAC 1406 1411 . . 1411 1416 + PstI CTGCAG 1415 1411 . . 1411 1416 + SfeI* CTRYAG 1411 1415 . . 1412 1415 + CviRI* TGCA 1413 1413 . . 1416 1420 + MboII GAAGA 1428 1427 . . 1420 1424 + TspDTI ATGAA 1435 1433 . . 1423 1428 + SspI AATATT 1425 1425 . . 1427 1430 + MseI TTAA 1427 1429 . . 1431 1434 + SetI ASST 1434 1430 . . 1431 1436 + BspMI ACCTGC 1440 1444 . . 1434 1437 + CviRI* TGCA 1435 1435 . . 1441 1444 + CviJI RGCY 1442 1442 . . 1446 1451 + ApoI RAATTY 1446 1450 . . 1447 1450 + TspEI AATT 1446 1450 . . 1449 1453 - TspDTI ATGAA 1439 1437 . . 1462 1467 + VspI ATTAAT 1463 1465 . . 1463 1466 + MseI TTAA 1463 1465 . . 1477 1487 + Hin4I GAYNNNNNVTC 1468 1463 1500 1495 1477 1480 + DpnI GATC 1478 1478 . . 1477 1480 + BstKTI GATC 1479 1477 . . 1477 1480 + MboI GATC 1476 1480 . . 1479 1484 + Hpy178III* TCNNGA 1480 1482 . . 1479 1484 + BetI* WCCGGW 1479 1483 . . 1479 1484 + BspMII* TCCGGA 1479 1483 . . 1480 1483 + HpaII CCGG 1480 1482 . . 1477 1481 - BinI* GGATC 1471 1472 . . 1483 1487 + PleI GAGTC 1491 1492 . . 1483 1487 + MlyI GAGTC 1492 1492 . . 1483 1487 + HinfI GANTC 1483 1486 . . 1477 1487 - Hin4I GAYNNNNNVTC 1500 1495 1468 1463 1489 1499 + BsiYI* CCNNNNNNNGG 1495 1492 . . 1510 1514 + DdeI CTNAG 1510 1513 . . 1522 1525 + MnlI CCTC 1532 1531 . . 1530 1533 + SetI ASST 1533 1529 . . 1547 1551 + TspDTI ATGAA 1562 1560 . . 1557 1560 + MnlI CCTC 1567 1566 . . 1559 1563 - TspGWI ACGGA 1549 1547 . . 1568 1573 - BsiI* CACGAG 1568 1572 . . 1580 1584 + TspDTI ATGAA 1595 1593 . . 1587 1591 + Tsp45I GTSAC 1586 1591 . . 1587 1591 + MaeIII GTNAC 1586 1591 . . 1588 1592 - HphI GGTGA 1580 1579 . . 1594 1598 + HinfI GANTC 1594 1597 . . 1594 1598 - PleI GAGTC 1588 1589 . . 1594 1598 - MlyI GAGTC 1588 1588 . . 1598 1602 + Hin4II* CCTTC 1608 1607 . . 1600 1604 - TspDTI ATGAA 1590 1588 . . 1610 1615 - NmeAIII GCCGAG 1590 1588 . . 1615 1618 + FatI CATG 1614 1618 . . 1615 1618 + NlaIII CATG 1618 1614 . . 1615 1618 + CviAII CATG 1615 1617 . . 1616 1620 + TspDTI ATGAA 1631 1629 . . 1626 1631 + TatI WGTACW 1626 1630 . . 1627 1630 + Csp6I GTAC 1627 1629 . . 1627 1630 + RsaI GTAC 1628 1628 . . 1636 1646 + BsaXI ACNNNNNCTCC 1626 1623 1656 1653 1650 1654 - BccI CCATC 1644 1645 . . 1679 1682 + TaqI TCGA 1679 1681 . . 1695 1699 + TfiI GAWTC 1695 1698 . . 1695 1699 + HinfI GANTC 1695 1698 . . 1703 1708 + Cfr10I RCCGGY 1703 1707 . . 1703 1708 + BetI* WCCGGW 1703 1707 . . 1703 1708 + AgeI ACCGGT 1703 1707 . . 1704 1707 + HpaII CCGG 1704 1706 . . 1706 1710 + BmgT120I GGNCC 1707 1708 . . 1706 1710 + AvaII GGWCC 1706 1709 . . 1706 1710 + AsuI* GGNCC 1706 1709 . . 1715 1718 + MnlI CCTC 1725 1724 . . 1718 1721 + MaeI CTAG 1718 1720 . . 1721 1726 + XhoII RGATCY 1721 1725 . . 1721 1726 + NlaIV GGNNCC 1723 1723 . . 1721 1726 + BamHI GGATCC 1721 1725 . . 1721 1725 + BinI* GGATC 1729 1730 . . 1722 1725 + DpnI GATC 1723 1723 . . 1722 1725 + BstKTI GATC 1724 1722 . . 1722 1725 + MboI GATC 1721 1725 . . 1722 1726 - BinI* GGATC 1716 1717 . . 1746 1750 + HphI GGTGA 1758 1757 . . 1753 1757 + Tsp45I GTSAC 1752 1757 . . 1753 1757 + MaeIII GTNAC 1752 1757 . . 1756 1760 + Tsp4CI* ACNGT 1758 1757 . . 1754 1758 - HphI GGTGA 1746 1745 . . 1781 1784 + SetI ASST 1784 1780 . . 1787 1792 + Hpy178III* TCNNGA 1788 1790 . . 1791 1795 + HinfI GANTC 1791 1794 . . 1791 1795 - PleI GAGTC 1785 1786 . . 1791 1795 - MlyI GAGTC 1785 1785 . . 1795 1798 + AciI CCGC 1795 1797 . . 1794 1799 - EciI GGCGGA 1784 1782 . . 1808 1813 + Hpy178III* TCNNGA 1809 1811 . . 1815 1818 + SetI ASST 1818 1814 . . 1832 1838 + SanDI GGGWCCC 1833 1836 . . 1832 1838 + PpuMI RGGWCCY 1833 1836 . . 1832 1838 + DraII RGGNCCY 1833 1836 . . 1832 1837 + NlaIV GGNNCC 1834 1834 . . 1833 1838 + NlaIV GGNNCC 1835 1835 . . 1833 1837 + BmgT120I GGNCC 1834 1835 . . 1833 1837 + AvaII GGWCC 1833 1836 . . 1833 1837 + AsuI* GGNCC 1833 1836 . . 1837 1841 + Hin4II* CCTTC 1847 1846 . . 1834 1838 - BslFI GGGAC 1819 1823 . . 1840 1843 + TaqI TCGA 1840 1842 . . 1847 1851 + MboII GAAGA 1859 1858 . . 1850 1854 + MboII GAAGA 1862 1861 . . 1856 1866 + BsiYI* CCNNNNNNNGG 1862 1859 . . 1864 1869 + StuI AGGCCT 1866 1866 . . 1865 1868 + CviJI RGCY 1866 1866 . . 1865 1868 + HaeIII GGCC 1866 1866 . . 1863 1866 - MnlI CCTC 1856 1855 . . 1867 1870 + MnlI CCTC 1877 1876 . . 1884 1888 + Tsp4CI* ACNGT 1886 1885 . . 1887 1891 + MaeIII GTNAC 1886 1891 . . 1897 1907 + BsiYI* CCNNNNNNNGG 1903 1900 . . 1894 1898 - HphI GGTGA 1886 1885 . . 1903 1907 - Hin4II* CCTTC 1897 1896 . . #--------------------------------------- # # # AatII: # [GACGTC] # 410 1525 # # Acc65I: # [GGTACC] # 672 1263 # # AccI: # [GTMKAC] # 920 1015 # # AciI: # [CCGC] # 25 140 584 1186 # # AclI: # [AACGTT] # 63 116 742 1014 # # AcyI: # [GRCGYC] # 407 723 805 # # AgeI: # [ACCGGT] # 232 1703 # # AhaIII*: # [TTTAAA] # 947 988 # # AjuI: # [GAANNNNNNNTTGG] # 32 457 1446 # # AloI: # [GAACNNNNNNTCC] # 32 565 1338 # # AluI: # [AGCT] # 72 167 304 540 852 # # ApoI: # [RAATTY] # 10 66 74 84 85 113 # 141 169 200 229 275 489 # # AsuI*: # [GGNCC] # 13 102 127 1693 # # AvaI: # [CYCGRG] # 896 1039 # # AvaII: # [GGWCC] # 13 102 127 1693 # # BamHI: # [GGATCC] # 214 1721 # # BbvII*: # [GAAGAC] # 788 1147 # # BccI: # [CCATC] # 4 291 487 542 611 # # BceAI: # [ACGGC] # 613 1322 # # BciVI: # [GTATCC] # 42 471 1422 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 763 1138 # # BetI*: # [WCCGGW] # 123 224 232 559 797 # # BfiI: # [ACTGGG] # 229 1706 # # BglII: # [AGATCT] # 19 1916 # # BinI*: # [GGATC] # 13 206 245 518 953 # # BmeT110I: # [CYCGRG] # 895 1040 # # BmgT120I: # [GGNCC] # 14 101 127 1693 # # BsaXI: # [ACNNNNNCTCC] # 30 279 1626 # # BseBI: # [CCWGG] # 9 620 1306 # # BseGI: # [GGATG] # 42 309 1584 # # BseMII: # [CTCAG] # 202 451 1282 # # BseSI: # [GKGCMC] # 797 1138 # # BsiI*: # [CACGAG] # 367 1568 # # BsiYI*: # [CCNNNNNNNGG] # 32 41 367 596 899 # # BslFI: # [GGGAC] # 116 573 1246 # # BsmAI: # [GTCTC] # 136 513 1286 # # BspCNI: # [CTCAG] # 204 450 1281 # # BspHI: # [TCATGA] # 951 984 # # BspMI: # [ACCTGC] # 495 1440 # # BspMII*: # [TCCGGA] # 456 1479 # # BsrI: # [ACTGG] # 32 224 1679 # # BssKI: # [CCNGG] # 9 622 1304 # # BssNAI: # [GTATAC] # 919 1016 # # BstKTI: # [GATC] # 22 147 211 245 344 966 # # Cac8I: # [GCNNGC] # 807 1128 # # Cfr10I: # [RCCGGY] # 232 1703 # # ClaI: # [ATCGAT] # 387 1548 # # Csp6I: # [GTAC] # 125 308 548 954 # # CspCI: # [CAANNNNNGTGG] # 35 545 1355 # # CviAII: # [CATG] # 320 449 503 663 # # CviJI: # [RGCY] # 6 11 12 17 40 47 # 49 52 69 110 118 186 # 186 213 395 424 # # CviRI*: # [TGCA] # 12 22 39 107 153 423 # 500 679 # # DdeI: # [CTNAG] # 201 225 236 414 425 434 # # DpnI: # [GATC] # 21 147 212 245 344 966 # # DraII: # [RGGNCCY] # 102 1833 # # DrdI: # [GACNNNNNNGTC] # 806 1129 # # DsaI*: # [CCRYGG] # 448 1487 # # EciI: # [GGCGGA] # 151 1784 # # EcoRI: # [GAATTC] # 74 229 275 530 827 # # EcoRII: # [CCWGG] # 9 622 1304 # # EcoRV: # [GATATC] # 261 765 909 # # FatI: # [CATG] # 321 448 503 663 # # FnuDII*: # [CGCG] # 586 1349 # # FokI: # [GGATG] # 49 309 1577 # # HaeIII: # [GGCC] # 69 1866 # # HgaI: # [GACGC] # 13 544 575 803 # # HgiCI*: # [GGYRCC] # 122 672 1141 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 32 32 # 32 32 32 94 211 319 # 325 391 435 # # Hin4II*: # [CCTTC] # 38 44 50 108 239 278 # 307 871 # # HindII: # [GTYRAC] # 207 1728 # # HindIII: # [AAGCTT] # 542 1393 # # HinfI: # [GANTC] # 17 77 96 101 111 144 # 161 418 810 # # HpaII: # [CCGG] # 110 123 224 231 559 688 # # HphI: # [GGTGA] # 12 49 128 166 229 1351 # # Hpy166II: # [GTNNAC] # 61 146 809 919 # # Hpy178III*: # [TCNNGA] # 9 10 13 18 21 71 # 126 136 158 195 196 235 # 308 439 # # Hpy188I: # [TCNGA] # 141 744 1050 # # Hpy99I: # [CGWCG] # 800 1135 # # KpnI: # [GGTACC] # 676 1259 # # MaeI: # [CTAG] # 9 217 279 340 486 604 # # MaeII: # [ACGT] # 63 74 111 116 117 440 # 1014 # # MaeIII: # [GTNAC] # 49 67 134 166 180 292 # 1047 # # MboI: # [GATC] # 19 147 214 245 344 966 # # MboII: # [GAAGA] # 3 3 73 99 120 159 # 174 427 431 446 # # McrI*: # [CGRYCG] # 656 1279 # # MfeI: # [CAATTG] # 305 743 887 # # MlyI: # [GAGTC] # 96 150 161 197 429 902 # # MmeI: # [TCCRAC] # 111 311 482 1031 # # MnlI: # [CCTC] # 21 33 33 35 58 108 # 131 158 181 195 255 314 # 413 # # MseI: # [TTAA] # 5 36 45 63 96 385 # 402 431 472 # # MwoI: # [GCNNNNNNNGC] # 88 252 792 803 # # NcoI: # [CCATGG] # 448 1487 # # NdeI: # [CATATG] # 515 1420 # # NlaIII: # [CATG] # 317 452 503 663 # # NlaIV: # [GGNNCC] # 1 15 100 111 122 228 # 431 927 # # NmeAIII: # [GCCGAG] # 345 1590 # # NspBII*: # [CMGCKG] # 749 1186 # # PfoI: # [TCCNGGA] # 622 1313 # # PleI: # [GAGTC] # 97 150 161 197 429 901 # # PpiI: # [GAACNNNNNCTC] # 32 169 1734 # # PpuMI: # [RGGWCCY] # 102 1833 # # PstI: # [CTGCAG] # 520 1415 # # RsaI: # [GTAC] # 126 307 548 954 # # SanDI: # [GGGWCCC] # 102 1833 # # ScrFI: # [CCNGG] # 9 620 1306 # # SduI: # [GDGCHC] # 797 1138 # # SecI*: # [CCNNGG] # 185 263 631 856 # # SetI: # [ASST] # 8 19 34 37 48 49 # 51 64 66 70 72 74 # 80 91 92 99 99 111 # 116 117 117 170 251 # # SfaNI: # [GCATC] # 791 1144 # # SfeI*: # [CTRYAG] # 41 189 524 1181 # # SmlI: # [CTYRAG] # 896 1039 # # SpeI: # [ACTAGT] # 627 1308 # # SspI: # [AATATT] # 306 336 510 783 # # StuI: # [AGGCCT] # 69 1866 # # StyI: # [CCWWGG] # 448 1487 # # SwaI: # [ATTTAAAT] # 947 988 # # TaiI: # [ACGT] # 66 74 111 116 117 440 # 1011 # # TaqI: # [TCGA] # 9 32 46 80 95 98 # 161 355 518 541 # # TaqII: # [GACCGA] # 642 1293 # # TatI: # [WGTACW] # 309 1626 # # TfiI: # [GAWTC] # 240 418 450 827 # # TsoI: # [TARCCA] # 243 1692 # # Tsp45I: # [GTSAC] # 166 183 247 292 1047 # # Tsp4CI*: # [ACNGT] # 29 49 128 274 384 1071 # # TspDTI: # [ATGAA] # 4 5 18 28 36 73 # 76 86 123 186 195 289 # 304 512 # # TspEI: # [AATT] # 8 10 16 21 34 36 # 41 42 43 47 51 55 # 60 64 66 66 74 79 # 83 84 92 92 113 169 # 489 # # TspGWI: # [ACGGA] # 386 667 882 # # TspRI: # [CASTGNN] # 556 1379 # # Tth111I: # [GACNNNGTC] # 202 1733 # # VspI: # [ATTAAT] # 132 390 402 472 539 # # XhoI: # [CTCGAG] # 896 1039 # # XhoII: # [RGATCY] # 19 214 1702 # # XmnI: # [GAANNNNTTC] # 74 1861 # # ZraI: # [GACGTC] # 408 1527 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1935 # Reported_sequences: 1 # Reported_hitcount: 426 #---------------------------------------