Restriction Fragments for BMS1/YPL217C



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The currently selected gene/sequence is :

BMS1/YPL217C on chromosome XVI from coordinates 143171 to 139620.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.4584.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 23 27 + Tsp4CI* ACNGT 25 24 . . 33 37 - Hin4II* CCTTC 27 26 . . 39 43 + MboII GAAGA 51 50 . . 59 62 + SetI ASST 62 58 . . 59 62 + CviJI RGCY 60 60 . . 59 62 + AluI AGCT 60 60 . . 60 70 + MwoI GCNNNNNNNGC 66 63 . . 63 73 + BsiYI* CCNNNNNNNGG 69 66 . . 81 84 + CviRI* TGCA 82 82 . . 86 91 + StuI AGGCCT 88 88 . . 87 90 + CviJI RGCY 88 88 . . 87 90 + HaeIII GGCC 88 88 . . 94 104 + MwoI GCNNNNNNNGC 100 97 . . 95 100 + DsaI* CCRYGG 95 99 . . 95 100 + SecI* CCNNGG 95 99 . . 94 98 - BceAI ACGGC 79 81 . . 100 104 + BlsI GCNGC 102 101 . . 100 104 + BisI GCNGC 101 102 . . 100 104 + BbvI GCAGC 112 116 . . 100 104 + TseI GCWGC 100 103 . . 102 105 + CviJI RGCY 103 103 . . 105 110 + SecI* CCNNGG 105 109 . . 106 110 + BssKI CCNGG 105 110 . . 106 110 + BseBI CCWGG 107 108 . . 106 110 + ScrFI CCNGG 107 108 . . 106 110 + EcoRII CCWGG 105 110 . . 108 111 + SetI ASST 111 107 . . 114 118 - BccI CCATC 108 109 . . 119 130 + BcgI CGANNNNNNTGC 108 106 142 140 128 131 + CviRI* TGCA 129 129 . . 133 136 + SetI ASST 136 132 . . 145 150 + Hpy166II GTNNAC 147 147 . . 161 164 + CviRI* TGCA 162 162 . . 162 167 + NspI RCATGY 166 162 . . 163 166 + FatI CATG 162 166 . . 163 166 + NlaIII CATG 166 162 . . 163 166 + CviAII CATG 163 165 . . 178 181 + DpnI GATC 179 179 . . 178 181 + BstKTI GATC 180 178 . . 178 181 + MboI GATC 177 181 . . 182 185 + Csp6I GTAC 182 184 . . 182 185 + RsaI GTAC 183 183 . . 188 193 + Eco57MI CTGRAG 209 207 . . 188 193 + Eco57I CTGAAG 209 207 . . 190 194 + MboII GAAGA 202 201 . . 196 199 + DpnI GATC 197 197 . . 196 199 + BstKTI GATC 198 196 . . 196 199 + MboI GATC 195 199 . . 199 202 + AciI CCGC 199 201 . . 196 200 - BinI* GGATC 190 191 . . 202 205 + MnlI CCTC 212 211 . . 198 203 - EciI GGCGGA 188 186 . . 213 217 + Hpy99I CGWCG 217 212 . . 225 230 + NlaIV GGNNCC 227 227 . . 226 230 + BmgT120I GGNCC 227 228 . . 226 230 + AvaII GGWCC 226 229 . . 226 230 + AsuI* GGNCC 226 229 . . 227 232 + Hpy166II GTNNAC 229 229 . . 231 234 + SetI ASST 234 230 . . 232 242 + EcoNI CCTNNNNNAGG 236 237 . . 232 236 + BssKI CCNGG 231 236 . . 232 236 + BseBI CCWGG 233 234 . . 232 236 + ScrFI CCNGG 233 234 . . 232 236 + EcoRII CCWGG 231 236 . . 232 242 + BsiYI* CCNNNNNNNGG 238 235 . . 255 259 + TfiI GAWTC 255 258 . . 255 259 + HinfI GANTC 255 258 . . 260 265 + NlaIV GGNNCC 262 262 . . 260 264 + BmgT120I GGNCC 261 262 . . 260 264 + AvaII GGWCC 260 263 . . 260 264 + AsuI* GGNCC 260 263 . . 258 263 - TaqII GACCGA 248 246 . . 263 273 + BsiYI* CCNNNNNNNGG 269 266 . . 264 267 + MnlI CCTC 274 273 . . 261 265 - BslFI GGGAC 246 250 . . 269 274 + Hpy178III* TCNNGA 270 272 . . 306 309 + SetI ASST 309 305 . . 306 312 + PpuMI RGGWCCY 307 310 . . 306 312 + DraII RGGNCCY 307 310 . . 307 311 + BmgT120I GGNCC 308 309 . . 307 311 + AvaII GGWCC 307 310 . . 307 311 + AsuI* GGNCC 307 310 . . 316 320 + Tsp4CI* ACNGT 318 317 . . 322 326 + BsmAI GTCTC 327 331 . . 329 334 + Hpy166II GTNNAC 331 331 . . 338 342 + MboII GAAGA 350 349 . . 338 343 + BbvII* GAAGAC 345 349 . . 339 349 + FalI AAGNNNNNCTT 330 325 362 357 339 349 - FalI AAGNNNNNCTT 362 357 330 325 357 362 + SduI GDGCHC 361 357 . . 357 362 + BseSI GKGCMC 361 357 . . 364 367 - AciI CCGC 364 366 . . 369 380 + BcgI CGANNNNNNTGC 358 356 392 390 370 373 + DpnI GATC 371 371 . . 370 373 + BstKTI GATC 372 370 . . 370 373 + MboI GATC 369 373 . . 372 376 + Hpy188I TCNGA 374 373 . . 375 380 + BsmI GAATGC 381 379 . . 379 384 + BtgZI GCGATG 394 398 . . 394 404 + MwoI GCNNNNNNNGC 400 397 . . 395 399 + DdeI CTNAG 395 398 . . 423 426 + TspEI AATT 422 426 . . 428 432 + Tsp4CI* ACNGT 430 429 . . 433 436 + TspEI AATT 432 436 . . 460 465 + EcoRI GAATTC 460 464 . . 460 465 + ApoI RAATTY 460 464 . . 461 464 + TspEI AATT 460 464 . . 466 469 + MseI TTAA 466 468 . . 469 474 + SspI AATATT 471 471 . . 485 489 + Tsp4CI* ACNGT 487 486 . . 498 503 + TatI WGTACW 498 502 . . 498 503 + ScaI AGTACT 500 500 . . 499 502 + Csp6I GTAC 499 501 . . 499 502 + RsaI GTAC 500 500 . . 510 513 + SetI ASST 513 509 . . 510 513 + CviJI RGCY 511 511 . . 510 513 + AluI AGCT 511 511 . . 519 524 + Hpy178III* TCNNGA 520 522 . . 523 526 + DpnI GATC 524 524 . . 523 526 + BstKTI GATC 525 523 . . 523 526 + MboI GATC 522 526 . . 529 534 + AhaIII* TTTAAA 531 531 . . 530 533 + MseI TTAA 530 532 . . 536 540 + DdeI CTNAG 536 539 . . 536 540 + BspCNI CTCAG 549 547 . . 536 540 + BseMII CTCAG 550 548 . . 540 545 + Hpy166II GTNNAC 542 542 . . 540 545 + HindII GTYRAC 542 542 . . 552 555 + SetI ASST 555 551 . . 552 555 + CviJI RGCY 553 553 . . 552 555 + AluI AGCT 553 553 . . 557 561 + DdeI CTNAG 557 560 . . 568 571 + MseI TTAA 568 570 . . 576 584 + AlwNI CAGNNNCTG 581 578 . . 578 582 + TfiI GAWTC 578 581 . . 578 582 + HinfI GANTC 578 581 . . 581 586 + Hpy178III* TCNNGA 582 584 . . 586 590 + TspGWI ACGGA 601 599 . . 600 603 + SetI ASST 603 599 . . 603 606 + CviRI* TGCA 604 604 . . 608 611 + TspEI AATT 607 611 . . 617 620 + SetI ASST 620 616 . . 631 636 + VspI ATTAAT 632 634 . . 632 635 + MseI TTAA 632 634 . . 640 643 + SetI ASST 643 639 . . 638 642 - Hin4II* CCTTC 632 631 . . 642 647 + BciVI GTATCC 653 652 . . 645 650 + Hpy178III* TCNNGA 646 648 . . 660 665 + AhaIII* TTTAAA 662 662 . . 661 664 + MseI TTAA 661 663 . . 665 668 + SetI ASST 668 664 . . 670 675 + Hpy178III* TCNNGA 671 673 . . 674 683 + BsaBI GATNNNNATC 678 678 . . 688 692 + TspDTI ATGAA 703 701 . . 691 696 + ApoI RAATTY 691 695 . . 692 695 + TspEI AATT 691 695 . . 695 699 + Hpy188I TCNGA 697 696 . . 700 711 + BstXI CCANNNNNNTGG 707 703 . . 701 710 + MslI CAYNNNNRTG 705 705 . . 703 706 + MseI TTAA 703 705 . . 711 714 - MnlI CCTC 704 703 . . 721 725 - FokI GGATG 707 711 . . 721 725 - BseGI GGATG 720 718 . . 735 738 + CviJI RGCY 736 736 . . 743 747 + TfiI GAWTC 743 746 . . 743 747 + HinfI GANTC 743 746 . . 754 757 + MseI TTAA 754 756 . . 762 767 + Hpy178III* TCNNGA 763 765 . . 762 767 + BetI* WCCGGW 762 766 . . 762 767 + BspMII* TCCGGA 762 766 . . 763 766 + HpaII CCGG 763 765 . . 760 764 - FokI GGATG 746 750 . . 760 764 - BseGI GGATG 759 757 . . 767 770 + TspEI AATT 766 770 . . 768 773 + VspI ATTAAT 769 771 . . 769 772 + MseI TTAA 769 771 . . 777 781 + HinfI GANTC 777 780 . . 779 784 + SmlI CTYRAG 779 783 . . 775 779 - BsmAI GTCTC 769 773 . . 777 781 - PleI GAGTC 771 772 . . 777 781 - MlyI GAGTC 771 771 . . 779 784 - Bce83I* CTTGAG 764 762 . . 785 795 + Hin4I GAYNNNNNVTC 776 771 808 803 788 791 + CviRI* TGCA 789 789 . . 794 797 + TaqI TCGA 794 796 . . 785 795 - Hin4I GAYNNNNNVTC 808 803 776 771 795 800 + McrI* CGRYCG 798 796 . . 796 807 + DrdI GACNNNNNNGTC 802 800 . . 798 801 + AciI CCGC 798 800 . . 816 821 - TsoI TARCCA 806 804 . . 824 827 + CviRI* TGCA 825 825 . . 827 831 + Tsp4CI* ACNGT 829 828 . . 830 833 + Csp6I GTAC 830 832 . . 830 833 + RsaI GTAC 831 831 . . 841 845 + Hin4II* CCTTC 851 850 . . 850 854 + BssKI CCNGG 849 854 . . 850 853 + HpaII CCGG 850 852 . . 850 854 + ScrFI CCNGG 851 852 . . 850 854 + CauII* CCSGG 851 852 . . 852 857 + SduI GDGCHC 856 852 . . 852 857 + BseSI GKGCMC 856 852 . . 854 865 + AlfI GCANNNNNNTGC 843 841 877 875 855 864 + MslI CAYNNNNRTG 859 859 . . 855 864 + OliI CACNNNNGTG 859 859 . . 857 860 + MaeI CTAG 857 859 . . 863 866 + CviRI* TGCA 864 864 . . 854 865 - AlfI GCANNNNNNTGC 877 875 843 841 880 884 + HphI GGTGA 892 891 . . 888 892 + DdeI CTNAG 888 891 . . 888 892 + BspCNI CTCAG 901 899 . . 888 892 + BseMII CTCAG 902 900 . . 900 903 + TspEI AATT 899 903 . . 908 911 + TspEI AATT 907 911 . . 915 920 + XhoII RGATCY 915 919 . . 916 919 + DpnI GATC 917 917 . . 916 919 + BstKTI GATC 918 916 . . 916 919 + MboI GATC 915 919 . . 920 923 + FatI CATG 919 923 . . 920 923 + NlaIII CATG 923 919 . . 920 923 + CviAII CATG 920 922 . . 916 920 - BinI* GGATC 910 911 . . 923 927 - BslFI GGGAC 908 912 . . 930 933 + SetI ASST 933 929 . . 943 951 + AlwNI CAGNNNCTG 948 945 . . 948 952 + BsrI ACTGG 953 951 . . 951 955 + FokI GGATG 964 968 . . 951 955 + BseGI GGATG 957 955 . . 963 966 + TaiI ACGT 966 962 . . 963 966 + SetI ASST 966 962 . . 963 966 + MaeII ACGT 963 965 . . 973 977 + TspDTI ATGAA 988 986 . . 979 983 + MboII GAAGA 991 990 . . 979 984 - Ksp632I* CTCTTC 974 977 . . 982 985 - MnlI CCTC 975 974 . . 990 993 + CviJI RGCY 991 991 . . 995 999 + TspGWI ACGGA 1010 1008 . . 1002 1005 + TspEI AATT 1001 1005 . . 1013 1016 + MnlI CCTC 1023 1022 . . 1025 1029 + MboII GAAGA 1037 1036 . . 1025 1030 + BbvII* GAAGAC 1032 1036 . . 1029 1032 + TaiI ACGT 1032 1028 . . 1029 1032 + SetI ASST 1032 1028 . . 1029 1032 + MaeII ACGT 1029 1031 . . 1036 1039 + SetI ASST 1039 1035 . . 1055 1058 + TspEI AATT 1054 1058 . . 1059 1069 + Hin4I GAYNNNNNVTC 1050 1045 1082 1077 1075 1079 + Hpy188I TCNGA 1077 1076 . . 1082 1086 + Hpy188I TCNGA 1084 1083 . . 1086 1089 + SetI ASST 1089 1085 . . 1081 1086 - MmeI TCCRAC 1062 1060 . . 1085 1088 - MnlI CCTC 1078 1077 . . 1095 1099 - BccI CCATC 1089 1090 . . 1104 1108 + FokI GGATG 1117 1121 . . 1104 1108 + BseGI GGATG 1110 1108 . . 1105 1109 - SfaNI GCATC 1095 1099 . . 1136 1140 + TspDTI ATGAA 1151 1149 . . 1138 1142 + MboII GAAGA 1150 1149 . . 1142 1145 + CviJI RGCY 1143 1143 . . 1138 1143 - Ksp632I* CTCTTC 1133 1136 . . 1138 1144 - SapI GCTCTTC 1133 1136 . . 1156 1160 + BssKI CCNGG 1155 1160 . . 1156 1160 + ScrFI CCNGG 1157 1158 . . 1156 1160 + CauII* CCSGG 1157 1158 . . 1157 1160 + HpaII CCGG 1157 1159 . . 1158 1163 + CfrI YGGCCR 1158 1162 . . 1159 1162 + CviJI RGCY 1160 1160 . . 1159 1162 + HaeIII GGCC 1160 1160 . . 1170 1173 + SetI ASST 1173 1169 . . 1171 1175 + HphI GGTGA 1183 1182 . . 1169 1172 - MnlI CCTC 1162 1161 . . 1174 1178 - Hin4II* CCTTC 1168 1167 . . 1178 1188 + BplI GAGNNNNNCTC 1169 1164 1201 1196 1187 1192 + Hpy178III* TCNNGA 1188 1190 . . 1187 1192 + BspHI TCATGA 1187 1191 . . 1188 1191 + FatI CATG 1187 1191 . . 1188 1191 + NlaIII CATG 1191 1187 . . 1188 1191 + CviAII CATG 1188 1190 . . 1178 1188 - BplI GAGNNNNNCTC 1201 1196 1169 1164 1196 1201 + Hpy166II GTNNAC 1198 1198 . . 1221 1224 + CviRI* TGCA 1222 1222 . . 1228 1233 + ApoI RAATTY 1228 1232 . . 1229 1232 + TspEI AATT 1228 1232 . . 1234 1238 - BccI CCATC 1228 1229 . . 1247 1250 + CviRI* TGCA 1248 1248 . . 1249 1254 + MfeI CAATTG 1249 1253 . . 1250 1253 + TspEI AATT 1249 1253 . . 1262 1266 + BceAI ACGGC 1278 1280 . . 1266 1272 + TspRI CASTGNN 1272 1263 . . 1268 1272 + DdeI CTNAG 1268 1271 . . 1271 1274 + SetI ASST 1274 1270 . . 1271 1274 + CviJI RGCY 1272 1272 . . 1271 1274 + AluI AGCT 1272 1272 . . 1268 1272 - BspCNI CTCAG 1260 1258 . . 1268 1272 - BseMII CTCAG 1259 1257 . . 1275 1280 + Hpy178III* TCNNGA 1276 1278 . . 1275 1280 + BspHI TCATGA 1275 1279 . . 1276 1279 + FatI CATG 1275 1279 . . 1276 1279 + NlaIII CATG 1279 1275 . . 1276 1279 + CviAII CATG 1276 1278 . . 1277 1281 + TspDTI ATGAA 1292 1290 . . 1274 1278 - TspDTI ATGAA 1264 1262 . . 1288 1291 + DpnI GATC 1289 1289 . . 1288 1291 + BstKTI GATC 1290 1288 . . 1288 1291 + MboI GATC 1287 1291 . . 1290 1295 + Hpy178III* TCNNGA 1291 1293 . . 1290 1295 + BspHI TCATGA 1290 1294 . . 1291 1294 + FatI CATG 1290 1294 . . 1291 1294 + NlaIII CATG 1294 1290 . . 1291 1294 + CviAII CATG 1291 1293 . . 1292 1296 + TspDTI ATGAA 1307 1305 . . 1294 1298 - Hin4II* CCTTC 1288 1287 . . 1299 1302 + FatI CATG 1298 1302 . . 1299 1302 + NlaIII CATG 1302 1298 . . 1299 1302 + CviAII CATG 1299 1301 . . 1302 1306 + FokI GGATG 1315 1319 . . 1302 1306 + BseGI GGATG 1308 1306 . . 1318 1322 + MboII GAAGA 1330 1329 . . 1330 1333 - MnlI CCTC 1323 1322 . . 1337 1341 + TspDTI ATGAA 1352 1350 . . 1339 1343 - Hin4II* CCTTC 1333 1332 . . 1355 1359 + MboII GAAGA 1367 1366 . . 1361 1365 - BsrI ACTGG 1361 1359 . . 1366 1369 + MseI TTAA 1366 1368 . . 1379 1384 + ApoI RAATTY 1379 1383 . . 1380 1383 + TspEI AATT 1379 1383 . . 1391 1394 + CviJI RGCY 1392 1392 . . 1393 1397 - BsrI ACTGG 1393 1391 . . 1398 1403 + Hpy178III* TCNNGA 1399 1401 . . 1402 1406 + MboII GAAGA 1414 1413 . . 1405 1409 + MboII GAAGA 1417 1416 . . 1405 1410 + BbvII* GAAGAC 1412 1416 . . 1408 1412 + HgaI GACGC 1417 1422 . . 1423 1426 + TspEI AATT 1422 1426 . . 1436 1440 + TspDTI ATGAA 1451 1449 . . 1438 1442 + MboII GAAGA 1450 1449 . . 1443 1446 + SetI ASST 1446 1442 . . 1461 1465 + Hpy99I CGWCG 1465 1460 . . 1463 1466 + TaiI ACGT 1466 1462 . . 1463 1466 + SetI ASST 1466 1462 . . 1463 1466 + MaeII ACGT 1463 1465 . . 1465 1470 + BsgI GTGCAG 1486 1484 . . 1466 1469 + CviRI* TGCA 1467 1467 . . 1462 1467 - BtrI CACGTC 1464 1464 . . 1471 1475 + HinfI GANTC 1471 1474 . . 1474 1478 + Hpy188I TCNGA 1476 1475 . . 1471 1475 - PleI GAGTC 1465 1466 . . 1471 1475 - MlyI GAGTC 1465 1465 . . 1475 1485 + BsiYI* CCNNNNNNNGG 1481 1478 . . 1480 1485 + StyI CCWWGG 1480 1484 . . 1480 1485 + SecI* CCNNGG 1480 1484 . . 1484 1488 + FokI GGATG 1497 1501 . . 1484 1488 + BseGI GGATG 1490 1488 . . 1485 1489 - BccI CCATC 1479 1480 . . 1495 1500 + ClaI ATCGAT 1496 1498 . . 1496 1499 + TaqI TCGA 1496 1498 . . 1510 1515 + Cfr10I RCCGGY 1510 1514 . . 1510 1515 + BetI* WCCGGW 1510 1514 . . 1510 1515 + AgeI ACCGGT 1510 1514 . . 1511 1514 + HpaII CCGG 1511 1513 . . 1513 1517 + HphI GGTGA 1525 1524 . . 1522 1527 + NlaIV GGNNCC 1524 1524 . . 1522 1527 + HgiCI* GGYRCC 1522 1526 . . 1532 1535 + TspEI AATT 1531 1535 . . 1536 1539 + SetI ASST 1539 1535 . . 1536 1539 + CviJI RGCY 1537 1537 . . 1536 1539 + AluI AGCT 1537 1537 . . 1547 1555 + AlwNI CAGNNNCTG 1552 1549 . . 1549 1553 + TfiI GAWTC 1549 1552 . . 1549 1553 + HinfI GANTC 1549 1552 . . 1552 1556 + Hpy188I TCNGA 1554 1553 . . 1555 1560 + ApoI RAATTY 1555 1559 . . 1556 1559 + TspEI AATT 1555 1559 . . 1561 1565 + MboII GAAGA 1573 1572 . . 1561 1566 - Ksp632I* CTCTTC 1556 1559 . . 1570 1574 + MboII GAAGA 1582 1581 . . 1583 1587 + BssKI CCNGG 1582 1587 . . 1583 1587 + BseBI CCWGG 1584 1585 . . 1583 1588 + SecI* CCNNGG 1583 1587 . . 1583 1587 + ScrFI CCNGG 1584 1585 . . 1583 1587 + EcoRII CCWGG 1582 1587 . . 1595 1600 + NspBII* CMGCKG 1597 1597 . . 1597 1601 + TauI GCSGC 1600 1597 . . 1597 1601 + BlsI GCNGC 1599 1598 . . 1597 1601 + BisI GCNGC 1598 1599 . . 1597 1600 - AciI CCGC 1597 1599 . . 1607 1610 + SetI ASST 1610 1606 . . 1607 1610 + CviJI RGCY 1608 1608 . . 1607 1610 + AluI AGCT 1608 1608 . . 1609 1614 + Eco57MI CTGRAG 1630 1628 . . 1609 1614 + Eco57I CTGAAG 1630 1628 . . 1611 1615 + MboII GAAGA 1623 1622 . . 1633 1639 + PpuMI RGGWCCY 1634 1637 . . 1633 1639 + DraII RGGNCCY 1634 1637 . . 1634 1638 + BmgT120I GGNCC 1635 1636 . . 1634 1638 + AvaII GGWCC 1634 1637 . . 1634 1638 + AsuI* GGNCC 1634 1637 . . 1636 1639 + SetI ASST 1639 1635 . . 1637 1641 + BssKI CCNGG 1636 1641 . . 1637 1641 + BseBI CCWGG 1638 1639 . . 1637 1641 + ScrFI CCNGG 1638 1639 . . 1637 1641 + EcoRII CCWGG 1636 1641 . . 1652 1655 + SetI ASST 1655 1651 . . 1652 1655 + CviJI RGCY 1653 1653 . . 1652 1655 + AluI AGCT 1653 1653 . . 1662 1665 + FatI CATG 1661 1665 . . 1662 1665 + NlaIII CATG 1665 1661 . . 1662 1665 + CviAII CATG 1662 1664 . . 1677 1680 + SetI ASST 1680 1676 . . 1679 1684 + Eco57MI CTGRAG 1700 1698 . . 1675 1679 - HphI GGTGA 1667 1666 . . 1679 1684 + Eco57I CTGAAG 1700 1698 . . 1681 1685 + MboII GAAGA 1693 1692 . . 1696 1699 + SetI ASST 1699 1695 . . 1694 1698 - Hin4II* CCTTC 1688 1687 . . 1701 1704 - MnlI CCTC 1694 1693 . . 1708 1711 - MnlI CCTC 1701 1700 . . 1713 1717 + Hpy99I CGWCG 1717 1712 . . 1733 1737 + Tsp45I GTSAC 1732 1737 . . 1733 1737 + MaeIII GTNAC 1732 1737 . . 1741 1745 + MboII GAAGA 1753 1752 . . 1744 1748 + MboII GAAGA 1756 1755 . . 1766 1771 + Hpy178III* TCNNGA 1767 1769 . . 1763 1767 - MboII GAAGA 1755 1754 . . 1764 1769 - Eco57MI CTGRAG 1749 1747 . . 1764 1769 - Eco57I CTGAAG 1749 1747 . . 1781 1784 + Csp6I GTAC 1781 1783 . . 1777 1781 - BccI CCATC 1771 1772 . . 1781 1784 + RsaI GTAC 1782 1782 . . 1783 1787 + Tsp4CI* ACNGT 1785 1784 . . 1786 1790 + MaeIII GTNAC 1785 1790 . . 1795 1798 - MnlI CCTC 1788 1787 . . 1810 1813 + FatI CATG 1809 1813 . . 1810 1813 + NlaIII CATG 1813 1809 . . 1810 1813 + CviAII CATG 1810 1812 . . 1812 1815 + CviRI* TGCA 1813 1813 . . 1844 1847 + TaqI TCGA 1844 1846 . . 1852 1857 + AsuII TTCGAA 1853 1855 . . 1853 1856 + TaqI TCGA 1853 1855 . . 1859 1862 + TspEI AATT 1858 1862 . . 1863 1866 + CviJI RGCY 1864 1864 . . 1862 1867 - TsoI TARCCA 1852 1850 . . 1887 1890 + CviRI* TGCA 1888 1888 . . 1885 1889 - SfaNI GCATC 1875 1879 . . 1901 1905 + TfiI GAWTC 1901 1904 . . 1901 1905 + HinfI GANTC 1901 1904 . . 1905 1908 + MnlI CCTC 1915 1914 . . 1905 1910 + SecI* CCNNGG 1905 1909 . . 1910 1913 + Hin6I GCGC 1910 1912 . . 1910 1913 + GlaI GCGC 1911 1911 . . 1910 1913 + HhaI GCGC 1912 1910 . . 1906 1911 - NmeAIII GCCGAG 1886 1884 . . 1912 1917 + Cac8I GCNNGC 1914 1914 . . 1923 1926 + SetI ASST 1926 1922 . . 1946 1950 + Tsp45I GTSAC 1945 1950 . . 1946 1950 + MaeIII GTNAC 1945 1950 . . 1948 1952 + HinfI GANTC 1948 1951 . . 1948 1952 - PleI GAGTC 1942 1943 . . 1948 1952 - MlyI GAGTC 1942 1942 . . 1955 1959 + TspDTI ATGAA 1970 1968 . . 1959 1962 + SetI ASST 1962 1958 . . 1957 1961 - Hin4II* CCTTC 1951 1950 . . 1966 1970 + MboII GAAGA 1978 1977 . . 1976 1980 + BceAI ACGGC 1992 1994 . . 1987 1991 + MboII GAAGA 1999 1998 . . 1989 1994 + XhoII RGATCY 1989 1993 . . 1989 1994 + BglII AGATCT 1989 1993 . . 1990 1993 + DpnI GATC 1991 1991 . . 1990 1993 + BstKTI GATC 1992 1990 . . 1990 1993 + MboI GATC 1989 1993 . . 1992 1997 + XbaI TCTAGA 1992 1996 . . 1992 1997 + Hpy178III* TCNNGA 1993 1995 . . 1993 1996 + MaeI CTAG 1993 1995 . . 1996 2000 + MboII GAAGA 2008 2007 . . 1999 2003 - BccI CCATC 1993 1994 . . 2003 2012 + XmnI GAANNNNTTC 2007 2007 . . 2008 2012 + Hin4II* CCTTC 2018 2017 . . 2011 2015 + Hpy188I TCNGA 2013 2012 . . 2023 2027 + MboII GAAGA 2035 2034 . . 2023 2028 + BbvII* GAAGAC 2030 2034 . . 2033 2037 + Tsp45I GTSAC 2032 2037 . . 2033 2037 + MaeIII GTNAC 2032 2037 . . 2049 2053 + FokI GGATG 2062 2066 . . 2049 2053 + BseGI GGATG 2055 2053 . . 2047 2050 - MnlI CCTC 2040 2039 . . 2055 2059 + FokI GGATG 2068 2072 . . 2055 2059 + BseGI GGATG 2061 2059 . . 2053 2056 - MnlI CCTC 2046 2045 . . 2057 2061 + TspDTI ATGAA 2072 2070 . . 2065 2069 + MboII GAAGA 2077 2076 . . 2065 2070 + BbvII* GAAGAC 2072 2076 . . 2089 2092 + TspEI AATT 2088 2092 . . 2091 2095 - TspDTI ATGAA 2081 2079 . . 2101 2104 + TspEI AATT 2100 2104 . . 2107 2111 - SfaNI GCATC 2097 2101 . . 2110 2113 - AciI CCGC 2110 2112 . . 2113 2116 - MnlI CCTC 2106 2105 . . 2128 2131 + MseI TTAA 2128 2130 . . 2129 2134 + TsoI TARCCA 2145 2143 . . 2132 2137 + DsaI* CCRYGG 2132 2136 . . 2132 2137 + StyI CCWWGG 2132 2136 . . 2132 2137 + NcoI CCATGG 2132 2136 . . 2132 2137 + SecI* CCNNGG 2132 2136 . . 2133 2136 + FatI CATG 2132 2136 . . 2133 2136 + NlaIII CATG 2136 2132 . . 2133 2136 + CviAII CATG 2133 2135 . . 2152 2156 + TspDTI ATGAA 2167 2165 . . 2154 2159 + BsmI GAATGC 2160 2158 . . 2158 2162 + BlsI GCNGC 2160 2159 . . 2158 2162 + BisI GCNGC 2159 2160 . . 2158 2162 + TseI GCWGC 2158 2161 . . 2160 2163 + CviRI* TGCA 2161 2161 . . 2158 2162 - BbvI GCAGC 2145 2149 . . 2166 2170 - Hin4II* CCTTC 2160 2159 . . 2177 2182 + BsaAI YACGTR 2179 2179 . . 2178 2181 + TaiI ACGT 2181 2177 . . 2178 2181 + SetI ASST 2181 2177 . . 2178 2181 + MaeII ACGT 2178 2180 . . 2180 2185 + Hpy166II GTNNAC 2182 2182 . . 2180 2185 + SduI GDGCHC 2184 2180 . . 2180 2185 + BseSI GKGCMC 2184 2180 . . 2180 2185 + ApaLI GTGCAC 2180 2184 . . 2180 2185 + HgiAI* GWGCWC 2184 2180 . . 2181 2184 + CviRI* TGCA 2182 2182 . . 2186 2189 + TspEI AATT 2185 2189 . . 2189 2192 + TaqI TCGA 2189 2191 . . 2189 2194 + Hpy178III* TCNNGA 2190 2192 . . 2197 2201 + MboII GAAGA 2209 2208 . . 2202 2205 + SetI ASST 2205 2201 . . 2203 2207 + HphI GGTGA 2215 2214 . . 2200 2204 - Hin4II* CCTTC 2194 2193 . . 2212 2217 + AhaIII* TTTAAA 2214 2214 . . 2213 2216 + MseI TTAA 2213 2215 . . 2218 2222 + MboII GAAGA 2230 2229 . . 2225 2229 + TspDTI ATGAA 2240 2238 . . 2234 2238 + TspDTI ATGAA 2249 2247 . . 2246 2249 + FatI CATG 2245 2249 . . 2246 2249 + NlaIII CATG 2249 2245 . . 2246 2249 + CviAII CATG 2246 2248 . . 2252 2256 + TspDTI ATGAA 2267 2265 . . 2257 2262 + SfeI* CTRYAG 2257 2261 . . 2265 2268 + CviJI RGCY 2266 2266 . . 2265 2268 + HaeIII GGCC 2266 2266 . . 2262 2266 - Hin4II* CCTTC 2256 2255 . . 2271 2276 + EcoRV GATATC 2273 2273 . . 2280 2284 + Tsp4CI* ACNGT 2282 2281 . . 2289 2292 + TspEI AATT 2288 2292 . . 2291 2294 + MseI TTAA 2291 2293 . . 2307 2312 + Hpy178III* TCNNGA 2308 2310 . . 2316 2320 + HinfI GANTC 2316 2319 . . 2319 2324 + Hpy178III* TCNNGA 2320 2322 . . 2316 2320 - PleI GAGTC 2310 2311 . . 2316 2320 - MlyI GAGTC 2310 2310 . . 2318 2323 - Eco57MI CTGRAG 2303 2301 . . 2318 2323 - GsuI CTGGAG 2303 2301 . . 2328 2331 + TaiI ACGT 2331 2327 . . 2328 2331 + SetI ASST 2331 2327 . . 2328 2331 + MaeII ACGT 2328 2330 . . 2336 2340 + TfiI GAWTC 2336 2339 . . 2336 2340 + HinfI GANTC 2336 2339 . . 2339 2342 + TaqI TCGA 2339 2341 . . 2343 2346 + SetI ASST 2346 2342 . . 2341 2345 - Hin4II* CCTTC 2335 2334 . . 2352 2355 + SetI ASST 2355 2351 . . 2352 2355 + CviJI RGCY 2353 2353 . . 2352 2355 + AluI AGCT 2353 2353 . . 2365 2368 + Csp6I GTAC 2365 2367 . . 2365 2368 + RsaI GTAC 2366 2366 . . 2366 2371 + SnaBI TACGTA 2368 2368 . . 2366 2371 + BsaAI YACGTR 2368 2368 . . 2367 2370 + TaiI ACGT 2370 2366 . . 2367 2370 + SetI ASST 2370 2366 . . 2367 2370 + MaeII ACGT 2367 2369 . . 2377 2382 + AsuII TTCGAA 2378 2380 . . 2378 2381 + TaqI TCGA 2378 2380 . . 2386 2390 - BslFI GGGAC 2371 2375 . . 2395 2400 + ApoI RAATTY 2395 2399 . . 2396 2399 + TspEI AATT 2395 2399 . . 2402 2407 + Hpy178III* TCNNGA 2403 2405 . . 2406 2411 + ApoI RAATTY 2406 2410 . . 2407 2410 + TspEI AATT 2406 2410 . . 2419 2424 + ApoI RAATTY 2419 2423 . . 2420 2423 + TspEI AATT 2419 2423 . . 2426 2431 + MfeI CAATTG 2426 2430 . . 2427 2430 + TspEI AATT 2426 2430 . . 2435 2440 - TaqII CACCCA 2425 2423 . . 2450 2455 + McrI* CGRYCG 2453 2451 . . 2451 2456 + TaqII GACCGA 2467 2465 . . 2478 2481 + CviJI RGCY 2479 2479 . . 2480 2484 + BssKI CCNGG 2479 2484 . . 2480 2484 + BseBI CCWGG 2481 2482 . . 2480 2484 + ScrFI CCNGG 2481 2482 . . 2480 2484 + EcoRII CCWGG 2479 2484 . . 2482 2485 + SetI ASST 2485 2481 . . 2489 2493 + MboII GAAGA 2501 2500 . . 2489 2494 + BbvII* GAAGAC 2496 2500 . . 2489 2502 + AjuI GAANNNNNNNTTGG 2481 2476 2513 2508 2487 2497 - Hin4I GAYNNNNNVTC 2510 2505 2478 2473 2508 2512 + MboII GAAGA 2520 2519 . . 2517 2521 + MboII GAAGA 2529 2528 . . 2517 2522 + BbvII* GAAGAC 2524 2528 . . 2527 2538 + DrdI GACNNNNNNGTC 2533 2531 . . 2531 2537 + TspRI CASTGNN 2537 2528 . . 2532 2536 + BsrI ACTGG 2537 2535 . . 2530 2541 - CspCI CAANNNNNGTGG 2554 2552 2519 2517 2549 2554 - MmeI TCCRAC 2530 2528 . . 2557 2560 + SetI ASST 2560 2556 . . 2558 2563 + NlaIV GGNNCC 2560 2560 . . 2555 2559 - Hin4II* CCTTC 2549 2548 . . 2574 2577 + TspEI AATT 2573 2577 . . 2590 2594 + HinfI GANTC 2590 2593 . . 2593 2598 + XbaI TCTAGA 2593 2597 . . 2593 2598 + Hpy178III* TCNNGA 2594 2596 . . 2590 2594 - PleI GAGTC 2584 2585 . . 2590 2594 - MlyI GAGTC 2584 2584 . . 2594 2597 + MaeI CTAG 2594 2596 . . 2609 2614 + BsmI GAATGC 2615 2613 . . 2619 2624 + Hpy166II GTNNAC 2621 2621 . . 2619 2624 + BssNAI GTATAC 2621 2621 . . 2619 2624 + AccI GTMKAC 2620 2622 . . 2647 2651 + TauI GCSGC 2650 2647 . . 2647 2651 + BlsI GCNGC 2649 2648 . . 2647 2651 + BisI GCNGC 2648 2649 . . 2649 2652 + CviJI RGCY 2650 2650 . . 2647 2650 - AciI CCGC 2647 2649 . . 2657 2661 + Tsp4CI* ACNGT 2659 2658 . . 2659 2664 + NlaIV GGNNCC 2661 2661 . . 2659 2663 + BmgT120I GGNCC 2660 2661 . . 2659 2663 + AvaII GGWCC 2659 2662 . . 2659 2663 + AsuI* GGNCC 2659 2662 . . 2660 2664 - BslFI GGGAC 2645 2649 . . 2682 2685 + SetI ASST 2685 2681 . . 2683 2693 + BsiYI* CCNNNNNNNGG 2689 2686 . . 2700 2703 + TspEI AATT 2699 2703 . . 2720 2724 + BssKI CCNGG 2719 2724 . . 2720 2723 + HpaII CCGG 2720 2722 . . 2720 2724 + ScrFI CCNGG 2721 2722 . . 2720 2724 + CauII* CCSGG 2721 2722 . . 2726 2730 + Tsp4CI* ACNGT 2728 2727 . . 2736 2739 + TspEI AATT 2735 2739 . . 2739 2742 + CviRI* TGCA 2740 2740 . . 2740 2744 + BlsI GCNGC 2742 2741 . . 2740 2744 + BisI GCNGC 2741 2742 . . 2740 2744 + BbvI GCAGC 2752 2756 . . 2740 2744 + TseI GCWGC 2740 2743 . . 2748 2751 + SetI ASST 2751 2747 . . 2758 2762 + MboII GAAGA 2770 2769 . . 2758 2763 - Ksp632I* CTCTTC 2753 2756 . . 2768 2771 + TaiI ACGT 2771 2767 . . 2768 2771 + SetI ASST 2771 2767 . . 2768 2771 + MaeII ACGT 2768 2770 . . 2771 2774 + MseI TTAA 2771 2773 . . 2773 2778 + SspI AATATT 2775 2775 . . 2781 2784 + TspEI AATT 2780 2784 . . 2786 2789 + MseI TTAA 2786 2788 . . 2794 2797 + MseI TTAA 2794 2796 . . 2798 2801 + TspEI AATT 2797 2801 . . 2819 2824 + ApoI RAATTY 2819 2823 . . 2820 2823 + TspEI AATT 2819 2823 . . 2840 2845 + PsiI TTATAA 2842 2842 . . 2849 2854 + BspLU11I* ACATGT 2849 2853 . . 2849 2854 + NspI RCATGY 2853 2849 . . 2849 2854 + AflIII ACRYGT 2849 2853 . . 2850 2853 + FatI CATG 2849 2853 . . 2850 2853 + NlaIII CATG 2853 2849 . . 2850 2853 + CviAII CATG 2850 2852 . . 2874 2877 + CviRI* TGCA 2875 2875 . . 2878 2881 + SetI ASST 2881 2877 . . 2884 2887 - MnlI CCTC 2877 2876 . . 2889 2894 + MstI* TGCGCA 2891 2891 . . 2890 2893 + Hin6I GCGC 2890 2892 . . 2890 2893 + GlaI GCGC 2891 2891 . . 2890 2893 + HhaI GCGC 2892 2890 . . 2895 2898 + DpnI GATC 2896 2896 . . 2895 2898 + BstKTI GATC 2897 2895 . . 2895 2898 + MboI GATC 2894 2898 . . 2902 2906 + Tsp4CI* ACNGT 2904 2903 . . 2905 2909 + BsmAI GTCTC 2910 2914 . . 2907 2912 + BmeT110I CYCGRG 2908 2910 . . 2907 2912 + AvaI CYCGRG 2907 2911 . . 2912 2917 + BciVI GTATCC 2923 2922 . . 2916 2921 + DsaI* CCRYGG 2916 2920 . . 2916 2921 + SecI* CCNNGG 2916 2920 . . 2915 2919 - TspGWI ACGGA 2905 2903 . . 2920 2924 + HphI GGTGA 2932 2931 . . 2933 2938 + SduI GDGCHC 2937 2933 . . 2933 2938 + BseSI GKGCMC 2937 2933 . . 2945 2948 + CviJI RGCY 2946 2946 . . 2947 2953 + SauI* CCTNAGG 2948 2951 . . 2948 2952 + DdeI CTNAG 2948 2951 . . 2948 2952 - BspCNI CTCAG 2940 2938 . . 2948 2952 - BseMII CTCAG 2939 2937 . . 2950 2953 - MnlI CCTC 2943 2942 . . 2954 2958 + Tsp45I GTSAC 2953 2958 . . 2954 2958 + MaeIII GTNAC 2953 2958 . . 2958 2963 + SfeI* CTRYAG 2958 2962 . . 2965 2969 + BlsI GCNGC 2967 2966 . . 2965 2969 + BisI GCNGC 2966 2967 . . 2965 2969 + BbvI GCAGC 2977 2981 . . 2965 2969 + TseI GCWGC 2965 2968 . . 2967 2970 + CviJI RGCY 2968 2968 . . 2974 2977 - MnlI CCTC 2967 2966 . . 3003 3006 + TspEI AATT 3002 3006 . . 3007 3011 + DdeI CTNAG 3007 3010 . . 3011 3014 + DpnI GATC 3012 3012 . . 3011 3014 + BstKTI GATC 3013 3011 . . 3011 3014 + MboI GATC 3010 3014 . . 3014 3017 + FatI CATG 3013 3017 . . 3014 3017 + NlaIII CATG 3017 3013 . . 3014 3017 + CviAII CATG 3014 3016 . . 3018 3023 + BciVI GTATCC 3029 3028 . . 3027 3030 + AciI CCGC 3027 3029 . . 3028 3031 + FnuDII* CGCG 3029 3029 . . 3032 3037 + Hpy178III* TCNNGA 3033 3035 . . 3029 3033 - HgaI GACGC 3018 3023 . . 3037 3042 + ApoI RAATTY 3037 3041 . . 3038 3041 + TspEI AATT 3037 3041 . . 3052 3056 + MaeIII GTNAC 3051 3056 . . 3055 3058 + SetI ASST 3058 3054 . . 3056 3059 + MnlI CCTC 3066 3065 . . 3069 3073 - Hin4II* CCTTC 3063 3062 . . 3090 3093 + SetI ASST 3093 3089 . . 3094 3097 + MseI TTAA 3094 3096 . . 3106 3109 + CviJI RGCY 3107 3107 . . 3106 3109 + HaeIII GGCC 3107 3107 . . 3118 3122 + TauI GCSGC 3121 3118 . . 3118 3122 + BlsI GCNGC 3120 3119 . . 3118 3122 + BisI GCNGC 3119 3120 . . 3120 3123 + CviJI RGCY 3121 3121 . . 3118 3121 - AciI CCGC 3118 3120 . . 3116 3121 - BsrBI CCGCTC 3118 3118 . . 3124 3128 + TspDTI ATGAA 3139 3137 . . 3128 3131 + SetI ASST 3131 3127 . . 3129 3133 + BssKI CCNGG 3128 3133 . . 3129 3133 + BseBI CCWGG 3130 3131 . . 3129 3133 + ScrFI CCNGG 3130 3131 . . 3129 3133 + EcoRII CCWGG 3128 3133 . . 3138 3142 + Tsp4CI* ACNGT 3140 3139 . . 3140 3144 + Hpy99I CGWCG 3144 3139 . . 3142 3145 + TaqI TCGA 3142 3144 . . 3144 3148 + TfiI GAWTC 3144 3147 . . 3144 3148 + HinfI GANTC 3144 3147 . . 3147 3152 + Hpy178III* TCNNGA 3148 3150 . . 3147 3152 + BetI* WCCGGW 3147 3151 . . 3147 3152 + BspMII* TCCGGA 3147 3151 . . 3148 3151 + HpaII CCGG 3148 3150 . . 3152 3156 + Tsp4CI* ACNGT 3154 3153 . . 3153 3159 + TspRI CASTGNN 3159 3150 . . 3156 3159 + CviRI* TGCA 3157 3157 . . 3174 3177 + TaiI ACGT 3177 3173 . . 3174 3177 + SetI ASST 3177 3173 . . 3174 3177 + MaeII ACGT 3174 3176 . . 3174 3179 + AflIII ACRYGT 3174 3178 . . 3187 3196 + MslI CAYNNNNRTG 3191 3191 . . 3203 3206 + SetI ASST 3206 3202 . . 3203 3209 + PpuMI RGGWCCY 3204 3207 . . 3203 3209 + DraII RGGNCCY 3204 3207 . . 3204 3209 + NlaIV GGNNCC 3206 3206 . . 3204 3208 + BmgT120I GGNCC 3205 3206 . . 3204 3208 + AvaII GGWCC 3204 3207 . . 3204 3208 + AsuI* GGNCC 3204 3207 . . 3205 3209 - BslFI GGGAC 3190 3194 . . 3213 3216 + SetI ASST 3216 3212 . . 3213 3216 + CviJI RGCY 3214 3214 . . 3213 3216 + AluI AGCT 3214 3214 . . 3227 3230 + TspEI AATT 3226 3230 . . 3242 3246 + DdeI CTNAG 3242 3245 . . 3242 3246 + BspCNI CTCAG 3255 3253 . . 3242 3246 + BseMII CTCAG 3256 3254 . . 3243 3247 + Hpy188I TCNGA 3245 3244 . . 3245 3250 + XhoII RGATCY 3245 3249 . . 3246 3249 + DpnI GATC 3247 3247 . . 3246 3249 + BstKTI GATC 3248 3246 . . 3246 3249 + MboI GATC 3245 3249 . . 3249 3253 + BccI CCATC 3257 3258 . . 3246 3250 - BinI* GGATC 3240 3241 . . 3256 3260 + TspDTI ATGAA 3271 3269 . . 3270 3274 + MboII GAAGA 3282 3281 . . 3285 3288 + CviJI RGCY 3286 3286 . . 3286 3296 + MwoI GCNNNNNNNGC 3292 3289 . . 3284 3289 - TsoI TARCCA 3274 3272 . . 3294 3297 + CviJI RGCY 3295 3295 . . 3296 3301 + DsaI* CCRYGG 3296 3300 . . 3296 3301 + SecI* CCNNGG 3296 3300 . . 3295 3299 - BceAI ACGGC 3280 3282 . . 3304 3307 + MaeI CTAG 3304 3306 . . 3306 3309 + SetI ASST 3309 3305 . . 3310 3314 + HphI GGTGA 3322 3321 . . 3314 3318 + TspDTI ATGAA 3329 3327 . . 3321 3325 - Hin4II* CCTTC 3315 3314 . . 3329 3332 + DpnI GATC 3330 3330 . . 3329 3332 + BstKTI GATC 3331 3329 . . 3329 3332 + MboI GATC 3328 3332 . . 3334 3338 - TspDTI ATGAA 3324 3322 . . 3357 3362 + AsuII TTCGAA 3358 3360 . . 3358 3361 + TaqI TCGA 3358 3360 . . 3363 3366 + CviJI RGCY 3364 3364 . . 3378 3381 - MnlI CCTC 3371 3370 . . 3381 3385 - Hin4II* CCTTC 3375 3374 . . 3393 3396 + CviJI RGCY 3394 3394 . . 3390 3394 - Hin4II* CCTTC 3384 3383 . . 3395 3398 + MaeI CTAG 3395 3397 . . 3398 3403 + Hpy166II GTNNAC 3400 3400 . . 3398 3403 + HindII GTYRAC 3400 3400 . . 3402 3405 + TaiI ACGT 3405 3401 . . 3402 3405 + SetI ASST 3405 3401 . . 3402 3405 + MaeII ACGT 3402 3404 . . 3422 3425 + TspEI AATT 3421 3425 . . 3426 3429 + SetI ASST 3429 3425 . . 3426 3429 + CviJI RGCY 3427 3427 . . 3426 3429 + AluI AGCT 3427 3427 . . 3428 3432 + DdeI CTNAG 3428 3431 . . 3432 3436 - BccI CCATC 3426 3427 . . 3436 3439 - MnlI CCTC 3429 3428 . . 3439 3443 + MboII GAAGA 3451 3450 . . 3514 3518 + HphI GGTGA 3526 3525 . . 3515 3519 + Tsp45I GTSAC 3514 3519 . . 3515 3519 + MaeIII GTNAC 3514 3519 . . 3512 3515 - AciI CCGC 3512 3514 . . 3517 3527 + Hin4I GAYNNNNNVTC 3508 3503 3540 3535 3521 3525 + TspDTI ATGAA 3536 3534 . . 3523 3527 + TfiI GAWTC 3523 3526 . . 3523 3527 + HinfI GANTC 3523 3526 . . 3517 3527 - Hin4I GAYNNNNNVTC 3540 3535 3508 3503 3530 3535 + Hpy166II GTNNAC 3532 3532 . . 3533 3538 + BsaAI YACGTR 3535 3535 . . 3534 3537 + TaiI ACGT 3537 3533 . . 3534 3537 + SetI ASST 3537 3533 . . 3534 3537 + MaeII ACGT 3534 3536 . . 3543 3546 - MnlI CCTC 3536 3535 . . 3547 3550 + SetI ASST 3550 3546 . . 3546 3549 - MnlI CCTC 3539 3538 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 932 2620 # # AciI: # [CCGC] # 40 91 165 199 380 394 # 434 513 537 799 # # AflIII: # [ACRYGT] # 325 378 2849 # # AgeI: # [ACCGGT] # 1510 2042 # # AhaIII*: # [TTTAAA] # 131 531 1338 1552 # # AjuI: # [GAANNNNNNNTTGG] # 32 1039 2481 # # AlfI: # [GCANNNNNNTGC] # 0 0 34 843 2675 # # AluI: # [AGCT] # 42 45 60 71 125 213 # 265 451 700 719 861 # # AlwNI: # [CAGNNNCTG] # 367 581 604 2000 # # ApaLI: # [GTGCAC] # 1372 2180 # # ApoI: # [RAATTY] # 11 13 151 176 218 231 # 400 460 515 537 840 # # AsuI*: # [GGNCC] # 34 47 226 348 545 1025 # 1327 # # AsuII: # [TTCGAA] # 194 525 980 1853 # # AvaI: # [CYCGRG] # 645 2907 # # AvaII: # [GGWCC] # 34 47 226 348 545 1025 # 1327 # # BbvI: # [GCAGC] # 112 225 575 607 2033 # # BbvII*: # [GAAGAC] # 28 42 345 380 424 618 # 687 1028 # # BccI: # [CCATC] # 108 126 139 169 222 251 # 292 981 1264 # # Bce83I*: # [CTTGAG] # 764 2788 # # BceAI: # [ACGGC] # 79 272 714 1199 1288 # # BcgI: # [CGANNNNNNTGC] # 34 34 108 216 3160 # # BciVI: # [GTATCC] # 106 523 653 2270 # # BetI*: # [WCCGGW] # 405 748 762 1637 # # BglII: # [AGATCT] # 1563 1989 # # BinI*: # [GGATC] # 190 312 720 2330 # # BisI: # [GCNGC] # 93 101 153 225 433 489 # 561 1497 # # BlsI: # [GCNGC] # 93 102 153 225 432 489 # 561 1497 # # BmeT110I: # [CYCGRG] # 644 2908 # # BmgT120I: # [GGNCC] # 34 47 227 347 545 1025 # 1327 # # BplI: # [GAGNNNNNCTC] # 0 0 32 1169 2351 # # BsaAI: # [YACGTR] # 17 189 1167 2179 # # BsaBI: # [GATNNNNATC] # 678 2874 # # BseBI: # [CCWGG] # 54 107 126 422 649 843 # 1351 # # BseGI: # [GGATG] # 6 39 153 182 198 198 # 565 720 1491 # # BseMII: # [CTCAG] # 296 317 352 357 550 1680 # # BseSI: # [GKGCMC] # 361 495 615 753 1328 # # BsgI: # [GTGCAG] # 1486 2066 # # BsiYI*: # [CCNNNNNNNGG] # 31 69 169 863 1208 1212 # # BslFI: # [GGGAC] # 246 274 362 545 662 1463 # # BsmAI: # [GTCTC] # 327 442 642 2141 # # BsmI: # [GAATGC] # 381 455 937 1779 # # BspCNI: # [CTCAG] # 297 315 352 359 549 1680 # # BspHI: # [TCATGA] # 15 88 1187 2262 # # BspLU11I*: # [ACATGT] # 703 2849 # # BspMII*: # [TCCGGA] # 405 762 2385 # # BsrBI: # [CCGCTC] # 434 3118 # # BsrI: # [ACTGG] # 32 408 953 1015 1144 # # BssKI: # [CCNGG] # 54 105 126 240 306 409 # 424 427 618 843 # # BssNAI: # [GTATAC] # 931 2621 # # BstKTI: # [GATC] # 18 83 116 153 174 180 # 221 235 372 393 702 905 # # BstXI: # [CCANNNNNNTGG] # 707 2845 # # BtgZI: # [GCGATG] # 394 3158 # # BtrI: # [CACGTC] # 1464 2088 # # Cac8I: # [GCNNGC] # 1638 1914 # # CauII*: # [CCSGG] # 306 831 851 1564 # # Cfr10I: # [RCCGGY] # 1510 2042 # # CfrI: # [YGGCCR] # 1158 2394 # # ClaI: # [ATCGAT] # 1496 2056 # # Csp6I: # [GTAC] # 182 317 331 584 951 1187 # # CspCI: # [CAANNNNNGTGG] # 35 998 2519 # # CviAII: # [CATG] # 8 15 88 113 148 163 # 164 268 323 363 538 604 # 757 # # CviJI: # [RGCY] # 9 14 15 17 22 28 # 30 33 42 45 60 69 # 71 72 87 93 112 120 # 125 126 139 145 152 171 # 183 211 255 296 402 408 # # CviRI*: # [TGCA] # 21 26 33 36 39 47 # 75 82 135 185 219 273 # 282 346 358 395 442 558 # # DdeI: # [CTNAG] # 21 59 124 141 186 235 # 331 380 395 1680 # # DpnI: # [GATC] # 18 83 116 153 174 179 # 222 235 372 393 702 905 # # DraII: # [RGGNCCY] # 307 348 1327 1570 # # DrdI: # [GACNNNNNNGTC] # 802 1019 1731 # # DsaI*: # [CCRYGG] # 95 256 380 784 2037 # # EciI: # [GGCGGA] # 188 3364 # # Eco57I: # [CTGAAG] # 49 70 209 1421 1803 # # Eco57MI: # [CTGRAG] # 49 70 209 554 1249 1421 # # EcoNI: # [CCTNNNNNAGG] # 236 3316 # # EcoRI: # [GAATTC] # 460 3092 # # EcoRII: # [CCWGG] # 54 105 126 424 649 843 # 1351 # # EcoRV: # [GATATC] # 1279 2273 # # FalI: # [AAGNNNNNCTT] # 0 0 32 330 3190 # # FatI: # [CATG] # 8 15 88 113 148 162 # 164 268 323 363 539 604 # 757 # # FnuDII*: # [CGCG] # 523 3029 # # FokI: # [GGATG] # 6 39 153 182 198 218 # 565 707 1484 # # GlaI: # [GCGC] # 661 980 1911 # # GsuI: # [CTGGAG] # 1249 2303 # # HaeIII: # [GGCC] # 88 445 841 1072 1106 # # HgaI: # [GACGC] # 534 1417 1601 # # HgiAI*: # [GWGCWC] # 1368 2184 # # HgiCI*: # [GGYRCC] # 1522 2030 # # HhaI: # [GCGC] # 660 980 1912 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 12 32 # 32 32 32 242 776 998 # 1396 # # Hin4II*: # [CCTTC] # 9 27 34 45 60 62 # 67 79 120 142 168 214 # 219 252 263 317 355 514 # 605 # # Hin6I: # [GCGC] # 662 980 1910 # # HindII: # [GTYRAC] # 152 542 2858 # # HinfI: # [GANTC] # 20 29 34 47 78 165 # 254 255 323 352 368 379 # 554 694 # # HpaII: # [CCGG] # 87 307 354 404 428 763 # 1209 # # HphI: # [GGTGA] # 26 142 204 291 342 390 # 548 717 892 # # Hpy166II: # [GTNNAC] # 20 82 102 132 147 211 # 439 656 779 984 # # Hpy178III*: # [TCNNGA] # 12 15 25 62 64 83 # 88 92 108 115 118 191 # 197 226 250 270 368 404 # 425 439 # # Hpy188I: # [TCNGA] # 7 78 307 323 374 380 # 392 459 1232 # # Hpy99I: # [CGWCG] # 217 252 408 1248 1427 # # Ksp632I*: # [CTCTTC] # 159 423 799 974 1197 # # MaeI: # [CTAG] # 91 157 601 710 857 1136 # # MaeII: # [ACGT] # 18 39 66 132 150 228 # 401 406 434 715 963 # # MaeIII: # [GTNAC] # 38 53 87 98 160 463 # 921 1732 # # MboI: # [GATC] # 18 83 116 153 174 177 # 224 235 372 393 702 905 # # MboII: # [GAAGA] # 1 2 3 9 9 9 # 19 21 21 27 33 37 # 41 42 46 47 51 60 # 70 101 113 123 132 148 # 151 169 180 222 241 271 # 512 641 # # McrI*: # [CGRYCG] # 798 1099 1655 # # MfeI: # [CAATTG] # 1126 1177 1249 # # MlyI: # [GAGTC] # 274 368 477 694 771 968 # # MmeI: # [TCCRAC] # 1022 1062 1468 # # MnlI: # [CCTC] # 3 6 7 13 24 48 # 55 58 60 62 66 84 # 87 99 107 125 127 161 # 212 271 305 371 430 771 # # MseI: # [TTAA] # 8 15 15 29 38 42 # 51 64 64 78 85 300 # 458 466 480 597 762 # # MslI: # [CAYNNNNRTG] # 154 361 705 2332 # # MstI*: # [TGCGCA] # 661 2891 # # MwoI: # [GCNNNNNNNGC] # 34 66 260 300 2892 # # NcoI: # [CCATGG] # 1420 2132 # # NlaIII: # [CATG] # 8 15 88 113 148 164 # 166 268 323 363 535 604 # 757 # # NlaIV: # [GGNNCC] # 35 101 227 346 545 1036 # 1262 # # NmeAIII: # [GCCGAG] # 1666 1886 # # NspBII*: # [CMGCKG] # 1597 1955 # # NspI: # [RCATGY] # 166 699 2687 # # OliI: # [CACNNNNGTG] # 859 2693 # # PleI: # [GAGTC] # 274 368 477 694 771 968 # # PpuMI: # [RGGWCCY] # 307 348 1327 1570 # # PsiI: # [TTATAA] # 710 2842 # # RsaI: # [GTAC] # 183 317 331 584 951 1186 # # SapI: # [GCTCTTC] # 1133 2419 # # SauI*: # [CCTNAGG] # 604 2948 # # ScaI: # [AGTACT] # 500 3052 # # ScrFI: # [CCNGG] # 54 107 126 240 306 409 # 422 427 618 843 # # SduI: # [GDGCHC] # 361 495 615 753 1328 # # SecI*: # [CCNNGG] # 10 95 103 227 256 322 # 380 784 1375 # # SetI: # [ASST] # 2 7 9 10 13 15 # 15 16 17 19 20 20 # 23 24 24 25 25 25 # 29 29 33 35 36 38 # 42 46 48 49 50 62 # 66 66 71 73 75 75 # 84 93 96 98 101 108 # 110 115 125 126 172 177 # 204 219 227 265 # # SfaNI: # [GCATC] # 222 780 1095 1455 # # SfeI*: # [CTRYAG] # 594 701 2257 # # SmlI: # [CTYRAG] # 779 2773 # # SnaBI: # [TACGTA] # 1184 2368 # # SspI: # [AATATT] # 471 777 2304 # # StuI: # [AGGCCT] # 88 3464 # # StyI: # [CCWWGG] # 652 1420 1480 # # TaiI: # [ACGT] # 15 39 66 132 150 228 # 401 406 434 715 966 # # TaqI: # [TCGA] # 9 39 150 194 216 336 # 348 702 764 794 # # TaqII: # [GACCGA] # 42 248 1085 2177 # # TatI: # [WGTACW] # 498 3054 # # TauI: # [GCSGC] # 431 471 1050 1600 # # TfiI: # [GAWTC] # 29 165 255 323 352 379 # 435 806 808 # # TseI: # [GCWGC] # 100 225 582 587 2058 # # TsoI: # [TARCCA] # 278 293 806 1046 1129 # # Tsp45I: # [GTSAC] # 38 87 213 561 921 1732 # # Tsp4CI*: # [ACNGT] # 14 25 57 69 112 176 # 236 293 342 377 398 497 # 956 # # TspDTI: # [ATGAA] # 5 9 9 15 16 18 # 28 45 53 73 86 99 # 102 113 132 163 207 285 # 519 703 872 # # TspEI: # [AATT] # 7 8 10 11 12 13 # 17 21 22 24 28 35 # 36 43 45 53 75 84 # 85 94 103 107 109 126 # 130 131 133 147 147 174 # 183 189 195 230 303 422 # # TspGWI: # [ACGGA] # 409 601 647 1895 # # TspRI: # [CASTGNN] # 393 622 1265 1272 # # VspI: # [ATTAAT] # 137 632 2783 # # XbaI: # [TCTAGA] # 601 959 1992 # # XhoII: # [RGATCY] # 307 915 1074 1256 # # XmnI: # [GAANNNNTTC] # 1545 2007 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 3552 # Reported_sequences: 1 # Reported_hitcount: 831 #---------------------------------------