Restriction Fragments for PGC1/YPL206C



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

PGC1/YPL206C on chromosome XVI from coordinates 163597 to 162632.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.14287.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 9 12 + TspEI AATT 8 12 . . 15 19 + BmgT120I GGNCC 16 17 . . 15 19 + AsuI* GGNCC 15 18 . . 16 19 + CviJI RGCY 17 17 . . 16 19 + HaeIII GGCC 17 17 . . 24 27 + SetI ASST 27 23 . . 24 27 + CviJI RGCY 25 25 . . 24 27 + AluI AGCT 25 25 . . 25 35 + MwoI GCNNNNNNNGC 31 28 . . 28 33 + AhaIII* TTTAAA 30 30 . . 29 32 + MseI TTAA 29 31 . . 38 43 + EcoRV GATATC 40 40 . . 42 47 + Hpy178III* TCNNGA 43 45 . . 58 61 + MaeI CTAG 58 60 . . 72 75 + CviJI RGCY 73 73 . . 69 73 - Hin4II* CCTTC 63 62 . . 73 83 + MwoI GCNNNNNNNGC 79 76 . . 78 81 + CviRI* TGCA 79 79 . . 79 89 + MwoI GCNNNNNNNGC 85 82 . . 79 89 + BstAPI GCANNNNNTGC 85 82 . . 79 83 + BlsI GCNGC 81 80 . . 79 83 + BisI GCNGC 80 81 . . 79 83 + BbvI GCAGC 91 95 . . 79 83 + TseI GCWGC 79 82 . . 80 85 + EcoP15I CAGCAG 110 112 . . 83 91 + AlwNI CAGNNNCTG 88 85 . . 84 87 + SetI ASST 87 83 . . 82 87 - BspMI ACCTGC 73 77 . . 82 88 - AarI CACCTGC 73 77 . . 103 107 + TspGWI ACGGA 118 116 . . 119 133 + XcmI CCANNNNNNNNNTGG 126 125 . . 122 127 + BtgZI GCGATG 137 141 . . 124 128 - BccI CCATC 118 119 . . 139 144 + Hpy166II GTNNAC 141 141 . . 145 148 + FatI CATG 144 148 . . 145 148 + NlaIII CATG 148 144 . . 145 148 + CviAII CATG 145 147 . . 148 152 + HinfI GANTC 148 151 . . 150 154 + DdeI CTNAG 150 153 . . 150 154 + BspCNI CTCAG 163 161 . . 150 154 + BseMII CTCAG 164 162 . . 151 155 + Hpy188I TCNGA 153 152 . . 148 152 - PleI GAGTC 142 143 . . 148 152 - MlyI GAGTC 142 142 . . 152 160 + AlwNI CAGNNNCTG 157 154 . . 154 163 + PshAI GACNNNNGTC 158 158 . . 156 162 + TspRI CASTGNN 162 153 . . 157 161 + BsrI ACTGG 162 160 . . 162 166 + Hpy188I TCNGA 164 163 . . 164 168 + FokI GGATG 177 181 . . 164 168 + BseGI GGATG 170 168 . . 161 166 - MmeI TCCRAC 142 140 . . 183 187 + HphI GGTGA 195 194 . . 193 197 + PleI GAGTC 201 202 . . 193 197 + MlyI GAGTC 202 202 . . 193 197 + HinfI GANTC 193 196 . . 195 200 + SalI GTCGAC 195 199 . . 195 200 + Hpy166II GTNNAC 197 197 . . 195 200 + HindII GTYRAC 197 197 . . 195 200 + AccI GTMKAC 196 198 . . 196 199 + TaqI TCGA 196 198 . . 205 210 + BseRI GAGGAG 220 218 . . 205 208 - MnlI CCTC 198 197 . . 209 212 + SetI ASST 212 208 . . 208 211 - MnlI CCTC 201 200 . . 212 215 + MseI TTAA 212 214 . . 217 228 + BcgI CGANNNNNNTGC 206 204 240 238 222 225 + Hin6I GCGC 222 224 . . 222 225 + GlaI GCGC 223 223 . . 222 225 + HhaI GCGC 224 222 . . 224 228 + BlsI GCNGC 226 225 . . 224 228 + BisI GCNGC 225 226 . . 224 228 + TseI GCWGC 224 227 . . 226 229 + CviRI* TGCA 227 227 . . 224 228 - BbvI GCAGC 211 215 . . 234 238 + FokI GGATG 247 251 . . 234 238 + BseGI GGATG 240 238 . . 232 235 - MnlI CCTC 225 224 . . 238 243 + NlaIV GGNNCC 240 240 . . 235 239 - BccI CCATC 229 230 . . 242 248 + Bpu10I CCTNAGC 243 246 . . 243 247 + DdeI CTNAG 243 246 . . 247 252 + BsrDI GCAATG 254 252 . . 261 265 - Hin4II* CCTTC 255 254 . . 266 271 + ApoI RAATTY 266 270 . . 267 270 + TspEI AATT 266 270 . . 275 278 + FatI CATG 274 278 . . 275 278 + NlaIII CATG 278 274 . . 275 278 + CviAII CATG 275 277 . . 280 285 + BtsI GCAGTG 287 285 . . 281 287 + TspRI CASTGNN 287 278 . . 288 292 + BccI CCATC 296 297 . . 291 297 + PfoI TCCNGGA 291 296 . . 292 296 + BssKI CCNGG 291 296 . . 292 296 + BseBI CCWGG 293 294 . . 292 296 + ScrFI CCNGG 293 294 . . 292 296 + EcoRII CCWGG 291 296 . . 289 293 - FokI GGATG 275 279 . . 289 293 - BseGI GGATG 288 286 . . 297 300 + SetI ASST 300 296 . . 297 300 + CviJI RGCY 298 298 . . 297 300 + AluI AGCT 298 298 . . 302 305 + TspEI AATT 301 305 . . 309 314 - MmeI TCCRAC 290 288 . . 319 324 + ApoI RAATTY 319 323 . . 320 323 + TspEI AATT 319 323 . . 341 346 + Hpy178III* TCNNGA 342 344 . . 341 346 + BspHI TCATGA 341 345 . . 342 345 + FatI CATG 341 345 . . 342 345 + NlaIII CATG 345 341 . . 342 345 + CviAII CATG 342 344 . . 344 349 + BclI TGATCA 344 348 . . 345 348 + DpnI GATC 346 346 . . 345 348 + BstKTI GATC 347 345 . . 345 348 + MboI GATC 344 348 . . 347 352 + Hpy178III* TCNNGA 348 350 . . 352 355 + SetI ASST 355 351 . . 363 366 + FatI CATG 362 366 . . 363 366 + NlaIII CATG 366 362 . . 363 366 + CviAII CATG 363 365 . . 371 374 + SetI ASST 374 370 . . 370 373 - MnlI CCTC 363 362 . . 374 377 + MseI TTAA 374 376 . . 382 385 + DpnI GATC 383 383 . . 382 385 + BstKTI GATC 384 382 . . 382 385 + MboI GATC 381 385 . . 386 389 + MseI TTAA 386 388 . . 388 393 + ApoI RAATTY 388 392 . . 389 392 + TspEI AATT 388 392 . . 404 408 + BinI* GGATC 412 413 . . 402 405 - MnlI CCTC 395 394 . . 405 408 + DpnI GATC 406 406 . . 405 408 + BstKTI GATC 407 405 . . 405 408 + MboI GATC 404 408 . . 408 413 + BsaAI YACGTR 410 410 . . 408 413 + PmaCI CACGTG 410 410 . . 409 412 + TaiI ACGT 412 408 . . 409 412 + SetI ASST 412 408 . . 409 412 + MaeII ACGT 409 411 . . 416 419 + CviJI RGCY 417 417 . . 417 427 + MwoI GCNNNNNNNGC 423 420 . . 435 438 + Csp6I GTAC 435 437 . . 435 438 + RsaI GTAC 436 436 . . 454 458 + BsrI ACTGG 459 457 . . 462 466 + Hpy188I TCNGA 464 463 . . 463 468 + Eco57MI CTGRAG 484 482 . . 463 468 + Eco57I CTGAAG 484 482 . . 464 475 + BdaI TGANNNNNNTCA 453 451 487 485 465 474 + XmnI GAANNNNTTC 469 469 . . 465 469 - Hin4II* CCTTC 459 458 . . 464 475 - BdaI TGANNNNNNTCA 487 485 453 451 489 499 + FalI AAGNNNNNCTT 480 475 512 507 489 499 - FalI AAGNNNNNCTT 512 507 480 475 502 512 + Hin4I GAYNNNNNVTC 493 488 525 520 502 507 + EcoRV GATATC 504 504 . . 509 512 + MnlI CCTC 519 518 . . 515 518 + TspEI AATT 514 518 . . 524 529 + HaeII RGCGCY 528 524 . . 524 529 + Eco47III AGCGCT 526 526 . . 525 528 + Hin6I GCGC 525 527 . . 525 528 + GlaI GCGC 526 526 . . 525 528 + HhaI GCGC 527 525 . . 536 542 + TspRI CASTGNN 542 533 . . 544 547 + DpnI GATC 545 545 . . 544 547 + BstKTI GATC 546 544 . . 544 547 + MboI GATC 543 547 . . 547 550 + MnlI CCTC 557 556 . . 544 548 - BinI* GGATC 538 539 . . 572 576 - HgaI GACGC 561 566 . . 583 587 + BsmAI GTCTC 588 592 . . 587 590 + MnlI CCTC 597 596 . . 585 590 - BseRI GAGGAG 576 574 . . 591 596 + Hpy166II GTNNAC 593 593 . . 588 593 - BsiI* CACGAG 588 592 . . 598 601 + CviJI RGCY 599 599 . . 602 605 + TspEI AATT 601 605 . . 621 624 + SetI ASST 624 620 . . 631 635 + TspDTI ATGAA 646 644 . . 633 637 + MboII GAAGA 645 644 . . 640 645 + EcoRV GATATC 642 642 . . 647 650 + SetI ASST 650 646 . . 661 665 + Tsp4CI* ACNGT 663 662 . . 664 669 + Hpy166II GTNNAC 666 666 . . 664 669 + HindII GTYRAC 666 666 . . 672 675 + SetI ASST 675 671 . . 674 679 + SfeI* CTRYAG 674 678 . . 698 701 + TspEI AATT 697 701 . . 703 713 + BsiYI* CCNNNNNNNGG 709 706 . . 708 713 + DsaI* CCRYGG 708 712 . . 708 713 + StyI CCWWGG 708 712 . . 708 713 + NcoI CCATGG 708 712 . . 708 713 + SecI* CCNNGG 708 712 . . 709 712 + FatI CATG 708 712 . . 709 712 + NlaIII CATG 712 708 . . 709 712 + CviAII CATG 709 711 . . 720 731 + TstI CACNNNNNNTCC 711 706 743 738 721 725 + TspGWI ACGGA 736 734 . . 723 727 + FokI GGATG 736 740 . . 723 727 + BseGI GGATG 729 727 . . 727 730 + DpnI GATC 728 728 . . 727 730 + BstKTI GATC 729 727 . . 727 730 + MboI GATC 726 730 . . 729 733 + Hpy188I TCNGA 731 730 . . 727 731 - BinI* GGATC 721 722 . . 732 735 + DpnI GATC 733 733 . . 732 735 + BstKTI GATC 734 732 . . 732 735 + MboI GATC 731 735 . . 752 757 + BtgZI GCGATG 767 771 . . 754 758 - BccI CCATC 748 749 . . 763 767 + Tsp4CI* ACNGT 765 764 . . 764 769 + DsaI* CCRYGG 764 768 . . 764 769 + SecI* CCNNGG 764 768 . . 771 775 + MboII GAAGA 783 782 . . 776 779 + CviJI RGCY 777 777 . . 782 787 - EcoP15I CAGCAG 754 756 . . 802 805 + Hin6I GCGC 802 804 . . 802 805 + GlaI GCGC 803 803 . . 802 805 + HhaI GCGC 804 802 . . 806 810 - BsrI ACTGG 806 804 . . 805 810 - BfiI ACTGGG 800 799 . . 814 818 + SfaNI GCATC 823 827 . . 818 823 + SfeI* CTRYAG 818 822 . . 827 830 + CviJI RGCY 828 828 . . 823 827 - BccI CCATC 817 818 . . 829 833 + DdeI CTNAG 829 832 . . 833 837 + TfiI GAWTC 833 836 . . 833 837 + HinfI GANTC 833 836 . . 838 841 + FatI CATG 837 841 . . 838 841 + NlaIII CATG 841 837 . . 838 841 + CviAII CATG 838 840 . . 855 860 + PsiI TTATAA 857 857 . . 861 865 - FokI GGATG 847 851 . . 861 865 - BseGI GGATG 860 858 . . 868 871 + CviRI* TGCA 869 869 . . 869 874 + Cac8I GCNNGC 871 871 . . 890 895 + NlaIV GGNNCC 892 892 . . 891 895 + BmgT120I GGNCC 892 893 . . 891 895 + AvaII GGWCC 891 894 . . 891 895 + AsuI* GGNCC 891 894 . . 899 902 + MseI TTAA 899 901 . . 908 911 - AciI CCGC 908 910 . . 942 946 + Hin4II* CCTTC 952 951 . . 944 949 + AsuII TTCGAA 945 947 . . 945 948 + TaqI TCGA 945 947 . . 953 957 - TspDTI ATGAA 943 941 . . #--------------------------------------- # # # AarI: # [CACCTGC] # 73 893 # # AccI: # [GTMKAC] # 196 770 # # AciI: # [CCGC] # 58 908 # # AhaIII*: # [TTTAAA] # 30 936 # # AluI: # [AGCT] # 25 273 668 # # AlwNI: # [CAGNNNCTG] # 69 88 809 # # ApoI: # [RAATTY] # 53 69 266 578 # # AsuI*: # [GGNCC] # 15 75 876 # # AsuII: # [TTCGAA] # 21 945 # # AvaII: # [GGWCC] # 75 891 # # BbvI: # [GCAGC] # 91 120 755 # # BccI: # [CCATC] # 67 69 111 118 149 452 # # BcgI: # [CGANNNNNNTGC] # 34 206 726 # # BclI: # [TGATCA] # 344 622 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 453 479 # # BfiI: # [ACTGGG] # 166 800 # # BinI*: # [GGATC] # 126 183 245 412 # # BisI: # [GCNGC] # 80 145 741 # # BlsI: # [GCNGC] # 81 145 740 # # BmgT120I: # [GGNCC] # 16 74 876 # # Bpu10I: # [CCTNAGC] # 243 723 # # BsaAI: # [YACGTR] # 410 556 # # BseBI: # [CCWGG] # 293 673 # # BseGI: # [GGATG] # 48 70 106 131 170 441 # # BseMII: # [CTCAG] # 164 802 # # BseRI: # [GAGGAG] # 220 356 390 # # BsiI*: # [CACGAG] # 378 588 # # BsiYI*: # [CCNNNNNNNGG] # 257 709 # # BsmAI: # [GTCTC] # 378 588 # # BspCNI: # [CTCAG] # 163 803 # # BspHI: # [TCATGA] # 341 625 # # BspMI: # [ACCTGC] # 73 893 # # BsrDI: # [GCAATG] # 254 712 # # BsrI: # [ACTGG] # 160 162 297 347 # # BssKI: # [CCNGG] # 291 675 # # BstAPI: # [GCANNNNNTGC] # 85 881 # # BstKTI: # [GATC] # 5 23 37 139 183 232 # 347 # # BtgZI: # [GCGATG] # 137 199 630 # # BtsI: # [GCAGTG] # 287 679 # # Cac8I: # [GCNNGC] # 95 871 # # Csp6I: # [GTAC] # 435 531 # # CviAII: # [CATG] # 21 67 128 129 130 145 # 346 # # CviJI: # [RGCY] # 8 17 48 51 119 138 # 178 182 225 # # CviRI*: # [TGCA] # 79 97 148 642 # # DdeI: # [CTNAG] # 93 137 150 586 # # DpnI: # [GATC] # 5 23 37 139 183 233 # 346 # # DsaI*: # [CCRYGG] # 56 202 708 # # Eco47III: # [AGCGCT] # 440 526 # # Eco57I: # [CTGAAG] # 482 484 # # Eco57MI: # [CTGRAG] # 482 484 # # EcoP15I: # [CAGCAG] # 110 212 644 # # EcoRII: # [CCWGG] # 291 675 # # EcoRV: # [GATATC] # 40 138 324 464 # # FalI: # [AAGNNNNNCTT] # 0 0 32 454 480 # # FatI: # [CATG] # 21 67 129 129 130 144 # 346 # # FokI: # [GGATG] # 28 70 111 119 177 461 # # GlaI: # [GCGC] # 163 223 277 303 # # HaeII: # [RGCGCY] # 438 528 # # HaeIII: # [GGCC] # 17 949 # # HgaI: # [GACGC] # 405 561 # # HhaI: # [GCGC] # 162 224 277 303 # # Hin4I: # [GAYNNNNNVTC] # 32 441 493 # # Hin4II*: # [CCTTC] # 14 63 192 204 493 # # Hin6I: # [GCGC] # 164 222 277 303 # # HindII: # [GTYRAC] # 197 300 469 # # HinfI: # [GANTC] # 45 133 148 640 # # HphI: # [GGTGA] # 195 771 # # Hpy166II: # [GTNNAC] # 56 73 141 300 396 # # Hpy178III*: # [TCNNGA] # 6 43 299 618 # # Hpy188I: # [TCNGA] # 11 153 235 267 300 # # MaeI: # [CTAG] # 58 908 # # MaeII: # [ACGT] # 409 557 # # MboI: # [GATC] # 5 23 37 139 183 235 # 344 # # MboII: # [GAAGA] # 138 183 645 # # MlyI: # [GAGTC] # 60 142 764 # # MmeI: # [TCCRAC] # 142 148 676 # # MnlI: # [CCTC] # 3 24 32 38 40 124 # 138 198 369 # # MseI: # [TTAA] # 12 29 67 162 183 513 # # MwoI: # [GCNNNNNNNGC] # 6 31 48 338 543 # # NcoI: # [CCATGG] # 258 708 # # NlaIII: # [CATG] # 21 67 125 129 130 148 # 346 # # NlaIV: # [GGNNCC] # 74 240 652 # # PfoI: # [TCCNGGA] # 291 675 # # PleI: # [GAGTC] # 59 142 765 # # PmaCI: # [CACGTG] # 410 556 # # PshAI: # [GACNNNNGTC] # 158 808 # # PsiI: # [TTATAA] # 109 857 # # RsaI: # [GTAC] # 436 530 # # SalI: # [GTCGAC] # 195 771 # # ScrFI: # [CCNGG] # 293 673 # # SecI*: # [CCNNGG] # 56 202 708 # # SetI: # [ASST] # 19 25 26 27 38 55 # 60 88 125 212 291 # # SfaNI: # [GCATC] # 143 823 # # SfeI*: # [CTRYAG] # 144 148 674 # # StyI: # [CCWWGG] # 258 708 # # TaiI: # [ACGT] # 412 554 # # TaqI: # [TCGA] # 21 196 749 # # TfiI: # [GAWTC] # 133 833 # # TseI: # [GCWGC] # 79 145 742 # # Tsp4CI*: # [ACNGT] # 102 201 663 # # TspDTI: # [ATGAA] # 23 297 646 # # TspEI: # [AATT] # 8 18 35 69 87 96 # 126 258 269 # # TspGWI: # [ACGGA] # 118 230 618 # # TspRI: # [CASTGNN] # 125 162 255 424 # # TstI: # [CACNNNNNNTCC] # 32 223 711 # # XcmI: # [CCANNNNNNNNNTGG] # 126 840 # # XmnI: # [GAANNNNTTC] # 469 497 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 966 # Reported_sequences: 1 # Reported_hitcount: 245 #---------------------------------------