Restriction Fragments for HHO1/YPL127C



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The currently selected gene/sequence is :

HHO1/YPL127C on chromosome XVI from coordinates 309604 to 308828.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.4904.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 8 + NlaIV GGNNCC 5 5 . . 3 8 + HgiCI* GGYRCC 3 7 . . 5 10 + TaqII CACCCA 21 19 . . 47 50 + CviJI RGCY 48 48 . . 48 53 + Cac8I GCNNGC 50 50 . . 51 54 + CviRI* TGCA 52 52 . . 83 88 + StyI CCWWGG 83 87 . . 83 88 + SecI* CCNNGG 83 87 . . 87 90 + CviJI RGCY 88 88 . . 87 90 + HaeIII GGCC 88 88 . . 88 92 + TauI GCSGC 91 88 . . 88 92 + BlsI GCNGC 90 89 . . 88 92 + BisI GCNGC 89 90 . . 89 92 + AciI CCGC 89 91 . . 95 100 + Hpy178III* TCNNGA 96 98 . . 104 109 + DsaI* CCRYGG 104 108 . . 104 109 + SecI* CCNNGG 104 108 . . 106 110 + BceAI ACGGC 122 124 . . 118 121 - MnlI CCTC 111 110 . . 122 127 + HindIII AAGCTT 122 126 . . 123 126 + SetI ASST 126 122 . . 123 126 + CviJI RGCY 124 124 . . 123 126 + AluI AGCT 124 124 . . 128 131 + MnlI CCTC 138 137 . . 137 141 + MaeIII GTNAC 136 141 . . 149 154 + BclI TGATCA 149 153 . . 150 153 + DpnI GATC 151 151 . . 150 153 + BstKTI GATC 152 150 . . 150 153 + MboI GATC 149 153 . . 160 165 + SduI GDGCHC 164 160 . . 160 165 + HgiJII* GRGCYC 164 160 . . 157 161 - Hin4II* CCTTC 151 150 . . 161 164 + CviJI RGCY 162 162 . . 166 170 + BceAI ACGGC 182 184 . . 168 171 + CviJI RGCY 169 169 . . 174 178 - Hin4II* CCTTC 168 167 . . 180 183 + TaiI ACGT 183 179 . . 180 183 + SetI ASST 183 179 . . 180 183 + MaeII ACGT 180 182 . . 187 192 + XhoII RGATCY 187 191 . . 187 192 + NlaIV GGNNCC 189 189 . . 187 192 + BamHI GGATCC 187 191 . . 187 191 + BinI* GGATC 195 196 . . 188 191 + DpnI GATC 189 189 . . 188 191 + BstKTI GATC 190 188 . . 188 191 + MboI GATC 187 191 . . 188 192 - BinI* GGATC 182 183 . . 191 195 - BsrI ACTGG 191 189 . . 199 202 + HpaII CCGG 199 201 . . 205 210 + SmlI CTYRAG 205 209 . . 206 211 + Hpy178III* TCNNGA 207 209 . . 205 210 - Bce83I* CTTGAG 190 188 . . 232 242 + BsiYI* CCNNNNNNNGG 238 235 . . 233 238 + McrI* CGRYCG 236 234 . . 233 238 + PvuI CGATCG 236 234 . . 234 237 + DpnI GATC 235 235 . . 234 237 + BstKTI GATC 236 234 . . 234 237 + MboI GATC 233 237 . . 237 241 + Hpy99I CGWCG 241 236 . . 239 243 + Hpy188I TCNGA 241 240 . . 241 246 + XhoII RGATCY 241 245 . . 241 246 + NlaIV GGNNCC 243 243 . . 241 246 + BamHI GGATCC 241 245 . . 241 245 + BinI* GGATC 249 250 . . 242 245 + DpnI GATC 243 243 . . 242 245 + BstKTI GATC 244 242 . . 242 245 + MboI GATC 241 245 . . 238 243 - MmeI TCCRAC 219 217 . . 245 248 + AciI CCGC 245 247 . . 242 246 - BinI* GGATC 236 237 . . 247 252 + Cac8I GCNNGC 249 249 . . 263 268 + TatI WGTACW 263 267 . . 264 267 + Csp6I GTAC 264 266 . . 264 267 + RsaI GTAC 265 265 . . 285 289 - Hin4II* CCTTC 279 278 . . 297 300 + CviJI RGCY 298 298 . . 297 300 + HaeIII GGCC 298 298 . . 298 303 + NaeI GCCGGC 300 300 . . 298 303 + MroNI GCCGGC 298 302 . . 295 298 - MnlI CCTC 288 287 . . 298 303 + Cac8I GCNNGC 300 300 . . 298 303 + Cfr10I RCCGGY 298 302 . . 299 302 + HpaII CCGG 299 301 . . 314 317 + CviJI RGCY 315 315 . . 321 327 + SanDI GGGWCCC 322 325 . . 321 327 + PpuMI RGGWCCY 322 325 . . 321 327 + DraII RGGNCCY 322 325 . . 321 325 + BslFI GGGAC 335 339 . . 321 326 + NlaIV GGNNCC 323 323 . . 322 327 + NlaIV GGNNCC 324 324 . . 322 326 + BmgT120I GGNCC 323 324 . . 322 326 + AvaII GGWCC 322 325 . . 322 326 + AsuI* GGNCC 322 325 . . 325 329 + FauI CCCGC 333 335 . . 326 331 + NspBII* CMGCKG 328 328 . . 326 329 + AciI CCGC 326 328 . . 342 346 + BsrI ACTGG 347 345 . . 344 349 + BalI TGGCCA 346 346 . . 344 349 + CfrI YGGCCR 344 348 . . 345 348 + CviJI RGCY 346 346 . . 345 348 + HaeIII GGCC 346 346 . . 351 355 + MboII GAAGA 363 362 . . 360 365 + Hpy178III* TCNNGA 361 363 . . 359 364 - Eco57MI CTGRAG 344 342 . . 359 364 - GsuI CTGGAG 344 342 . . 383 386 + SetI ASST 386 382 . . 382 385 - MnlI CCTC 375 374 . . 402 407 + Cac8I GCNNGC 404 404 . . 405 408 + CviJI RGCY 406 406 . . 406 410 + TauI GCSGC 409 406 . . 406 410 + BlsI GCNGC 408 407 . . 406 410 + BisI GCNGC 407 408 . . 407 410 + AciI CCGC 407 409 . . 411 420 + MslI CAYNNNNRTG 415 415 . . 411 420 + OliI CACNNNNGTG 415 415 . . 423 426 + CviRI* TGCA 424 424 . . 428 431 + AciI CCGC 428 430 . . 430 434 + SfaNI GCATC 439 443 . . 430 440 + MwoI GCNNNNNNNGC 436 433 . . 439 449 + MwoI GCNNNNNNNGC 445 442 . . 444 447 + CviJI RGCY 445 445 . . 445 449 + TauI GCSGC 448 445 . . 445 449 + BlsI GCNGC 447 446 . . 445 449 + BisI GCNGC 446 447 . . 446 449 + AciI CCGC 446 448 . . 448 452 + SfaNI GCATC 457 461 . . 449 453 - FokI GGATG 435 439 . . 449 453 - BseGI GGATG 448 446 . . 458 461 + SetI ASST 461 457 . . 458 461 + CviJI RGCY 459 459 . . 458 461 + AluI AGCT 459 459 . . 459 464 + Cac8I GCNNGC 461 461 . . 459 464 + BmtI GCTAGC 463 459 . . 459 464 + NheI GCTAGC 459 463 . . 460 463 + MaeI CTAG 460 462 . . 462 467 + HaeII RGCGCY 466 462 . . 463 466 + Hin6I GCGC 463 465 . . 463 466 + GlaI GCGC 464 464 . . 463 466 + HhaI GCGC 465 463 . . 496 500 + Tsp4CI* ACNGT 498 497 . . 499 503 + MaeIII GTNAC 498 503 . . 503 506 + AciI CCGC 503 505 . . 505 515 + MwoI GCNNNNNNNGC 511 508 . . 505 515 + BglI GCCNNNNNGGC 511 508 . . 512 517 + StuI AGGCCT 514 514 . . 513 516 + CviJI RGCY 514 514 . . 513 516 + HaeIII GGCC 514 514 . . 510 514 - Hin4II* CCTTC 504 503 . . 515 518 + MnlI CCTC 525 524 . . 516 521 + Ksp632I* CTCTTC 522 525 . . 517 521 - MboII GAAGA 509 508 . . 523 527 + Hin4II* CCTTC 533 532 . . 526 530 - MboII GAAGA 518 517 . . 528 532 - TspDTI ATGAA 518 516 . . 535 538 + SetI ASST 538 534 . . 549 552 + DpnI GATC 550 550 . . 549 552 + BstKTI GATC 551 549 . . 549 552 + MboI GATC 548 552 . . 549 553 - BinI* GGATC 543 544 . . 554 557 + MseI TTAA 554 556 . . 560 565 + NspI RCATGY 564 560 . . 560 565 + Cac8I GCNNGC 562 562 . . 560 565 + SphI GCATGC 564 560 . . 561 564 + FatI CATG 560 564 . . 561 564 + NlaIII CATG 564 560 . . 561 564 + CviAII CATG 561 563 . . 565 568 + MnlI CCTC 575 574 . . 572 575 + MseI TTAA 572 574 . . 578 582 + Tsp4CI* ACNGT 580 579 . . 585 590 + SduI GDGCHC 589 585 . . 585 590 + HgiJII* GRGCYC 589 585 . . 586 589 + CviJI RGCY 587 587 . . 586 591 + NlaIV GGNNCC 588 588 . . 592 595 + CviJI RGCY 593 593 . . 588 593 - Eco57MI CTGRAG 573 571 . . 588 593 - GsuI CTGGAG 573 571 . . 593 597 - BceAI ACGGC 578 580 . . 603 608 + AhaIII* TTTAAA 605 605 . . 604 607 + MseI TTAA 604 606 . . 622 632 + Hin4I GAYNNNNNVTC 613 608 645 640 623 633 + BsaXI ACNNNNNCTCC 613 610 643 640 632 635 + MnlI CCTC 642 641 . . 630 635 - BseRI GAGGAG 621 619 . . 649 652 + SetI ASST 652 648 . . 649 652 + CviJI RGCY 650 650 . . 649 652 + AluI AGCT 650 650 . . 654 659 + ApoI RAATTY 654 658 . . 655 658 + TspEI AATT 654 658 . . 676 681 + HaeII RGCGCY 680 676 . . 676 681 + Eco47III AGCGCT 678 678 . . 677 680 + Hin6I GCGC 677 679 . . 677 680 + GlaI GCGC 678 678 . . 677 680 + HhaI GCGC 679 677 . . 683 688 + Hpy178III* TCNNGA 684 686 . . 701 705 + BceAI ACGGC 717 719 . . 713 716 + CviRI* TGCA 714 714 . . 722 728 + DraII RGGNCCY 723 726 . . 723 729 + DraII RGGNCCY 724 727 . . 723 728 + SduI GDGCHC 727 723 . . 723 728 + Bsp120I GGGCCC 723 727 . . 723 728 + NlaIV GGNNCC 725 725 . . 723 727 + BmgT120I GGNCC 724 725 . . 723 728 + HgiJII* GRGCYC 727 723 . . 723 728 + BseSI GKGCMC 727 723 . . 723 727 + AsuI* GGNCC 723 726 . . 723 728 + ApaI GGGCCC 727 723 . . 724 727 + CviJI RGCY 725 725 . . 724 727 + HaeIII GGCC 725 725 . . 724 729 + NlaIV GGNNCC 726 726 . . 724 728 + BmgT120I GGNCC 725 726 . . 724 728 + AsuI* GGNCC 724 727 . . 725 735 + MwoI GCNNNNNNNGC 731 728 . . 725 735 + BglI GCCNNNNNGGC 731 728 . . 728 731 + MnlI CCTC 738 737 . . 731 734 + HpaII CCGG 731 733 . . 740 743 + MseI TTAA 740 742 . . 753 757 + MboII GAAGA 765 764 . . 758 761 + SetI ASST 761 757 . . 756 760 - Hin4II* CCTTC 750 749 . . 771 776 + BsaAI YACGTR 773 773 . . 772 775 + TaiI ACGT 775 771 . . 772 775 + SetI ASST 775 771 . . 772 775 + MaeII ACGT 772 774 . . #--------------------------------------- # # # AciI: # [CCGC] # 18 21 57 81 81 89 # 156 274 # # AhaIII*: # [TTTAAA] # 172 605 # # AluI: # [AGCT] # 124 127 191 335 # # ApaI: # [GGGCCC] # 50 727 # # ApoI: # [RAATTY] # 123 654 # # AsuI*: # [GGNCC] # 1 53 322 401 # # AvaII: # [GGWCC] # 322 455 # # BalI: # [TGGCCA] # 346 431 # # BamHI: # [GGATCC] # 54 187 536 # # Bce83I*: # [CTTGAG] # 190 587 # # BceAI: # [ACGGC] # 60 60 122 139 396 # # BclI: # [TGATCA] # 149 628 # # BglI: # [GCCNNNNNGGC] # 46 220 511 # # BinI*: # [GGATC] # 13 13 41 182 234 294 # # BisI: # [GCNGC] # 39 89 318 331 # # BlsI: # [GCNGC] # 39 90 318 330 # # BmgT120I: # [GGNCC] # 1 52 323 401 # # BmtI: # [GCTAGC] # 314 463 # # BsaAI: # [YACGTR] # 4 773 # # BsaXI: # [ACNNNNNCTCC] # 30 134 613 # # BseGI: # [GGATG] # 329 448 # # BseRI: # [GAGGAG] # 156 621 # # BseSI: # [GKGCMC] # 50 727 # # BsiYI*: # [CCNNNNNNNGG] # 238 539 # # BslFI: # [GGGAC] # 335 442 # # Bsp120I: # [GGGCCC] # 54 723 # # BsrI: # [ACTGG] # 156 191 430 # # BstKTI: # [GATC] # 8 38 46 152 226 307 # # Cac8I: # [GCNNGC] # 50 51 57 101 104 199 # 215 # # Cfr10I: # [RCCGGY] # 298 479 # # CfrI: # [YGGCCR] # 344 433 # # Csp6I: # [GTAC] # 264 513 # # CviAII: # [CATG] # 216 561 # # CviJI: # [RGCY] # 6 7 14 17 31 36 # 38 39 40 48 52 55 # 57 60 73 75 129 # # CviRI*: # [TGCA] # 52 63 290 372 # # DpnI: # [GATC] # 8 38 46 151 227 307 # # DraII: # [RGGNCCY] # 1 53 322 401 # # DsaI*: # [CCRYGG] # 104 673 # # Eco47III: # [AGCGCT] # 99 678 # # Eco57MI: # [CTGRAG] # 204 229 344 # # FatI: # [CATG] # 217 560 # # FauI: # [CCCGC] # 333 444 # # FokI: # [GGATG] # 342 435 # # GlaI: # [GCGC] # 99 214 464 # # GsuI: # [CTGGAG] # 204 229 344 # # HaeII: # [RGCGCY] # 97 214 466 # # HaeIII: # [GGCC] # 48 52 88 168 210 211 # # HgiCI*: # [GGYRCC] # 3 774 # # HgiJII*: # [GRGCYC] # 50 138 164 425 # # HhaI: # [GCGC] # 98 214 465 # # Hin4I: # [GAYNNNNNVTC] # 32 132 613 # # Hin4II*: # [CCTTC] # 17 27 29 111 151 217 # 225 # # Hin6I: # [GCGC] # 100 214 463 # # HindIII: # [AAGCTT] # 122 655 # # HpaII: # [CCGG] # 46 100 199 432 # # Hpy178III*: # [TCNNGA] # 93 96 111 154 323 # # Hpy188I: # [TCNGA] # 241 536 # # Hpy99I: # [CGWCG] # 241 536 # # Ksp632I*: # [CTCTTC] # 255 522 # # MaeI: # [CTAG] # 317 460 # # MaeII: # [ACGT] # 5 180 592 # # MaeIII: # [GTNAC] # 136 279 362 # # MboI: # [GATC] # 8 38 46 149 229 307 # # MboII: # [GAAGA] # 9 12 146 247 363 # # McrI*: # [CGRYCG] # 236 541 # # MmeI: # [TCCRAC] # 219 558 # # MnlI: # [CCTC] # 27 39 50 67 87 96 # 111 150 150 # # MroNI: # [GCCGGC] # 298 479 # # MseI: # [TTAA] # 18 32 37 136 554 # # MslI: # [CAYNNNNRTG] # 362 415 # # MwoI: # [GCNNNNNNNGC] # 9 46 66 220 436 # # NaeI: # [GCCGGC] # 300 477 # # NheI: # [GCTAGC] # 318 459 # # NlaIII: # [CATG] # 213 564 # # NlaIV: # [GGNNCC] # 1 1 5 51 54 80 # 137 184 264 # # NspBII*: # [CMGCKG] # 328 449 # # NspI: # [RCATGY] # 213 564 # # OliI: # [CACNNNNGTG] # 362 415 # # PpuMI: # [RGGWCCY] # 322 455 # # PvuI: # [CGATCG] # 236 541 # # RsaI: # [GTAC] # 265 512 # # SanDI: # [GGGWCCC] # 322 455 # # SduI: # [GDGCHC] # 50 138 164 425 # # SecI*: # [CCNNGG] # 21 83 673 # # SetI: # [ASST] # 2 14 57 75 77 109 # 114 126 203 # # SfaNI: # [GCATC] # 18 320 439 # # SmlI: # [CTYRAG] # 205 572 # # SphI: # [GCATGC] # 213 564 # # StuI: # [AGGCCT] # 263 514 # # StyI: # [CCWWGG] # 83 694 # # TaiI: # [ACGT] # 2 183 592 # # TaqII: # [CACCCA] # 21 756 # # TatI: # [WGTACW] # 263 514 # # TauI: # [GCSGC] # 39 91 318 329 # # Tsp4CI*: # [ACNGT] # 82 197 498 # # TspDTI: # [ATGAA] # 259 518 # # TspEI: # [AATT] # 123 654 # # XhoII: # [RGATCY] # 54 187 536 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 777 # Reported_sequences: 1 # Reported_hitcount: 223 #---------------------------------------