Restriction Fragments for NAN1/YPL126W



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The currently selected gene/sequence is :

NAN1/YPL126W on chromosome XVI from coordinates 310210 to 312900.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.17945.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 7 + HgaI GACGC 12 17 . . 12 17 + StyI CCWWGG 12 16 . . 12 17 + SecI* CCNNGG 12 16 . . 12 17 + AvrII CCTAGG 12 16 . . 13 16 + MaeI CTAG 13 15 . . 15 18 + SetI ASST 18 14 . . 20 23 + TaqI TCGA 20 22 . . 24 28 + Tsp4CI* ACNGT 26 25 . . 33 37 + Tsp4CI* ACNGT 35 34 . . 58 62 + FauI CCCGC 66 68 . . 59 62 + AciI CCGC 59 61 . . 65 76 + BdaI TGANNNNNNTCA 54 52 88 86 73 77 + DdeI CTNAG 73 76 . . 73 77 + BspCNI CTCAG 86 84 . . 73 77 + BseMII CTCAG 87 85 . . 65 76 - BdaI TGANNNNNNTCA 88 86 54 52 82 86 + MaeIII GTNAC 81 86 . . 85 90 + Cfr10I RCCGGY 85 89 . . 86 89 + HpaII CCGG 86 88 . . 103 108 + Cac8I GCNNGC 105 105 . . 104 108 + FauI CCCGC 112 114 . . 105 108 + AciI CCGC 105 107 . . 143 146 + MseI TTAA 143 145 . . 150 154 + Hpy188I TCNGA 152 151 . . 153 156 + DpnI GATC 154 154 . . 153 156 + BstKTI GATC 155 153 . . 153 156 + MboI GATC 152 156 . . 158 161 + SetI ASST 161 157 . . 176 179 + MaeI CTAG 176 178 . . 188 191 + MseI TTAA 188 190 . . 192 196 + HinfI GANTC 192 195 . . 192 196 - PleI GAGTC 186 187 . . 192 196 - MlyI GAGTC 186 186 . . 204 207 + CviRI* TGCA 205 205 . . 223 228 + Hpy178III* TCNNGA 224 226 . . 223 228 + BetI* WCCGGW 223 227 . . 223 228 + BspMII* TCCGGA 223 227 . . 224 227 + HpaII CCGG 224 226 . . 228 233 + SspI AATATT 230 230 . . 236 239 + CviRI* TGCA 237 237 . . 241 245 + MboII GAAGA 253 252 . . 244 248 + MboII GAAGA 256 255 . . 244 249 - Ksp632I* CTCTTC 239 242 . . 254 258 + TspDTI ATGAA 269 267 . . 256 260 + TfiI GAWTC 256 259 . . 256 260 + HinfI GANTC 256 259 . . 258 263 + ClaI ATCGAT 259 261 . . 259 262 + TaqI TCGA 259 261 . . 270 274 + TfiI GAWTC 270 273 . . 270 274 + HinfI GANTC 270 273 . . 274 279 - EcoP15I CAGCAG 246 248 . . 280 284 + HphI GGTGA 292 291 . . 276 281 - GsaI CCCAGC 280 276 . . 276 281 - BseYI CCCAGC 276 280 . . 278 283 - TaqII CACCCA 268 266 . . 289 293 + Tsp4CI* ACNGT 291 290 . . 295 298 + AciI CCGC 295 297 . . 304 308 + MboII GAAGA 316 315 . . 304 309 + BbvII* GAAGAC 311 315 . . 307 311 + HgaI GACGC 316 321 . . 315 319 + Hpy188I TCNGA 317 316 . . 322 326 + Tsp4CI* ACNGT 324 323 . . 329 333 - HphI GGTGA 321 320 . . 343 346 + FatI CATG 342 346 . . 343 346 + NlaIII CATG 346 342 . . 343 346 + CviAII CATG 343 345 . . 348 351 + TspEI AATT 347 351 . . 354 359 + AhaIII* TTTAAA 356 356 . . 355 358 + MseI TTAA 355 357 . . 358 361 + TspEI AATT 357 361 . . 360 365 + PsiI TTATAA 362 362 . . 371 374 + SetI ASST 374 370 . . 371 374 + CviJI RGCY 372 372 . . 371 374 + AluI AGCT 372 372 . . 377 380 + TaqI TCGA 377 379 . . 379 383 + PleI GAGTC 387 388 . . 379 383 + MlyI GAGTC 388 388 . . 379 383 + HinfI GANTC 379 382 . . 381 385 - BslFI GGGAC 366 370 . . 385 391 + Bpu10I CCTNAGC 386 389 . . 386 390 + DdeI CTNAG 386 389 . . 413 417 + TspDTI ATGAA 428 426 . . 418 423 + HindIII AAGCTT 418 422 . . 419 422 + SetI ASST 422 418 . . 419 422 + CviJI RGCY 420 420 . . 419 422 + AluI AGCT 420 420 . . 420 425 + Cac8I GCNNGC 422 422 . . 423 426 + CviRI* TGCA 424 424 . . 435 445 + BsiYI* CCNNNNNNNGG 441 438 . . 442 445 - MnlI CCTC 435 434 . . 450 455 + SfeI* CTRYAG 450 454 . . 455 460 + ApoI RAATTY 455 459 . . 456 459 + TspEI AATT 455 459 . . 461 472 + BdaI TGANNNNNNTCA 450 448 484 482 465 470 + AclI AACGTT 466 468 . . 466 469 + TaiI ACGT 469 465 . . 466 469 + SetI ASST 469 465 . . 466 469 + MaeII ACGT 466 468 . . 461 472 - BdaI TGANNNNNNTCA 484 482 450 448 473 479 + PpuMI RGGWCCY 474 477 . . 473 479 + DraII RGGNCCY 474 477 . . 474 479 + NlaIV GGNNCC 476 476 . . 474 478 + BmgT120I GGNCC 475 476 . . 474 478 + AvaII GGWCC 474 477 . . 474 478 + AsuI* GGNCC 474 477 . . 475 484 + PshAI GACNNNNGTC 479 479 . . 479 482 + MaeI CTAG 479 481 . . 503 506 + CviRI* TGCA 504 504 . . 510 513 + Csp6I GTAC 510 512 . . 510 513 + RsaI GTAC 511 511 . . 514 517 + SetI ASST 517 513 . . 521 526 + EcoT22I ATGCAT 525 521 . . 522 525 + CviRI* TGCA 523 523 . . 526 529 + MseI TTAA 526 528 . . 529 532 + SetI ASST 532 528 . . 538 542 - SfaNI GCATC 528 532 . . 551 554 + TspEI AATT 550 554 . . 555 566 + BdaI TGANNNNNNTCA 544 542 578 576 561 564 + CviJI RGCY 562 562 . . 562 572 + MwoI GCNNNNNNNGC 568 565 . . 555 566 - BdaI TGANNNNNNTCA 578 576 544 542 583 588 + AclI AACGTT 584 586 . . 584 587 + TaiI ACGT 587 583 . . 584 587 + SetI ASST 587 583 . . 584 587 + MaeII ACGT 584 586 . . 598 603 + SspI AATATT 600 600 . . 604 608 - MboII GAAGA 596 595 . . 614 619 + Hpy166II GTNNAC 616 616 . . 621 626 + Cac8I GCNNGC 623 623 . . 621 626 + BmtI GCTAGC 625 621 . . 621 626 + NheI GCTAGC 621 625 . . 622 625 + MaeI CTAG 622 624 . . 625 628 + Hin6I GCGC 625 627 . . 625 628 + GlaI GCGC 626 626 . . 625 628 + HhaI GCGC 627 625 . . 628 633 + NdeI CATATG 629 631 . . 634 637 + CviRI* TGCA 635 635 . . 640 645 + ZraI GACGTC 642 642 . . 640 645 + AcyI GRCGYC 641 643 . . 640 645 + AatII GACGTC 644 640 . . 641 644 + TaiI ACGT 644 640 . . 641 644 + SetI ASST 644 640 . . 641 644 + MaeII ACGT 641 643 . . 642 647 + Esp3I CGTCTC 648 652 . . 643 647 + BsmAI GTCTC 648 652 . . 655 658 + DpnI GATC 656 656 . . 655 658 + BstKTI GATC 657 655 . . 655 658 + MboI GATC 654 658 . . 657 662 + Hpy178III* TCNNGA 658 660 . . 671 675 + BccI CCATC 679 680 . . 675 679 + DdeI CTNAG 675 678 . . 675 679 + BspCNI CTCAG 688 686 . . 675 679 + BseMII CTCAG 689 687 . . 697 701 + TfiI GAWTC 697 700 . . 697 701 + HinfI GANTC 697 700 . . 705 710 + ApoI RAATTY 705 709 . . 706 709 + TspEI AATT 705 709 . . 709 712 + MseI TTAA 709 711 . . 711 716 + HindIII AAGCTT 711 715 . . 712 715 + SetI ASST 715 711 . . 712 715 + CviJI RGCY 713 713 . . 712 715 + AluI AGCT 713 713 . . 720 723 + MnlI CCTC 730 729 . . 720 725 + SecI* CCNNGG 720 724 . . 724 729 + NlaIV GGNNCC 726 726 . . 728 732 + BccI CCATC 736 737 . . 729 733 - FokI GGATG 715 719 . . 729 733 - BseGI GGATG 728 726 . . 736 740 - MboII GAAGA 728 727 . . 744 748 + HinfI GANTC 744 747 . . 744 748 - PleI GAGTC 738 739 . . 744 748 - MlyI GAGTC 738 738 . . 769 772 + SetI ASST 772 768 . . 771 774 + Csp6I GTAC 771 773 . . 771 774 + RsaI GTAC 772 772 . . 773 776 + TaiI ACGT 776 772 . . 773 776 + SetI ASST 776 772 . . 773 776 + MaeII ACGT 773 775 . . 779 782 + MaeI CTAG 779 781 . . 783 786 + FatI CATG 782 786 . . 783 786 + NlaIII CATG 786 782 . . 783 786 + CviAII CATG 783 785 . . 785 790 + BalI TGGCCA 787 787 . . 785 790 + CfrI YGGCCR 785 789 . . 786 789 + CviJI RGCY 787 787 . . 786 789 + HaeIII GGCC 787 787 . . 788 802 + XcmI CCANNNNNNNNNTGG 795 794 . . 803 808 + Hpy166II GTNNAC 805 805 . . 803 808 + HindII GTYRAC 805 805 . . 810 814 + BsrI ACTGG 815 813 . . 813 816 + CviJI RGCY 814 814 . . 817 822 - MmeI TCCRAC 798 796 . . 827 830 + MnlI CCTC 837 836 . . 829 834 + Hpy178III* TCNNGA 830 832 . . 829 834 + BetI* WCCGGW 829 833 . . 829 834 + BspMII* TCCGGA 829 833 . . 830 833 + HpaII CCGG 830 832 . . 836 841 + PsiI TTATAA 838 838 . . 850 853 + CviJI RGCY 851 851 . . 852 856 + BssKI CCNGG 851 856 . . 852 856 + BseBI CCWGG 853 854 . . 852 856 + ScrFI CCNGG 853 854 . . 852 856 + EcoRII CCWGG 851 856 . . 854 859 + CfrI YGGCCR 854 858 . . 855 858 + CviJI RGCY 856 856 . . 855 858 + HaeIII GGCC 856 856 . . 859 862 + DpnI GATC 860 860 . . 859 862 + BstKTI GATC 861 859 . . 859 862 + MboI GATC 858 862 . . 876 886 + MwoI GCNNNNNNNGC 882 879 . . 892 896 + HinfI GANTC 892 895 . . 892 896 - PleI GAGTC 886 887 . . 892 896 - MlyI GAGTC 886 886 . . 903 907 + Tsp45I GTSAC 902 907 . . 903 907 + MaeIII GTNAC 902 907 . . 918 923 + Hpy178III* TCNNGA 919 921 . . 919 930 + TstI CACNNNNNNTCC 910 905 942 937 925 930 + XhoII RGATCY 925 929 . . 925 930 + NlaIV GGNNCC 927 927 . . 925 930 + BamHI GGATCC 925 929 . . 925 929 + BinI* GGATC 933 934 . . 926 929 + DpnI GATC 927 927 . . 926 929 + BstKTI GATC 928 926 . . 926 929 + MboI GATC 925 929 . . 922 926 - BccI CCATC 916 917 . . 926 930 - BinI* GGATC 920 921 . . 973 978 + MfeI CAATTG 973 977 . . 974 977 + TspEI AATT 973 977 . . 995 998 + TspEI AATT 994 998 . . 1003 1006 + MnlI CCTC 1013 1012 . . 1013 1017 + Tsp4CI* ACNGT 1015 1014 . . 1019 1024 + PsiI TTATAA 1021 1021 . . 1025 1028 + TaqI TCGA 1025 1027 . . 1028 1032 + Tsp4CI* ACNGT 1030 1029 . . 1034 1037 + SetI ASST 1037 1033 . . 1041 1047 + PpuMI RGGWCCY 1042 1045 . . 1041 1047 + DraII RGGNCCY 1042 1045 . . 1041 1045 + BslFI GGGAC 1055 1059 . . 1041 1046 + NlaIV GGNNCC 1043 1043 . . 1042 1046 + BmgT120I GGNCC 1043 1044 . . 1042 1046 + AvaII GGWCC 1042 1045 . . 1042 1046 + AsuI* GGNCC 1042 1045 . . 1044 1047 + SetI ASST 1047 1043 . . 1045 1048 + MnlI CCTC 1055 1054 . . 1046 1051 + SmlI CTYRAG 1046 1050 . . 1046 1051 - Bce83I* CTTGAG 1031 1029 . . 1053 1064 + PpiI GAACNNNNNCTC 1045 1040 1077 1072 1066 1069 + MseI TTAA 1066 1068 . . 1090 1093 + TspEI AATT 1089 1093 . . 1095 1100 + ApoI RAATTY 1095 1099 . . 1096 1099 + TspEI AATT 1095 1099 . . 1099 1103 + Hpy188I TCNGA 1101 1100 . . 1109 1112 + TspEI AATT 1108 1112 . . 1122 1127 + BsmI GAATGC 1128 1126 . . 1129 1133 + Hpy188I TCNGA 1131 1130 . . 1132 1142 + Hin4I GAYNNNNNVTC 1123 1118 1155 1150 1138 1141 + SetI ASST 1141 1137 . . 1139 1142 + MnlI CCTC 1149 1148 . . 1145 1148 + TspEI AATT 1144 1148 . . 1150 1153 + TaqI TCGA 1150 1152 . . 1154 1157 + MseI TTAA 1154 1156 . . 1159 1163 + BmgT120I GGNCC 1160 1161 . . 1159 1163 + AsuI* GGNCC 1159 1162 . . 1160 1163 + CviJI RGCY 1161 1161 . . 1160 1163 + HaeIII GGCC 1161 1161 . . 1167 1172 + Cfr10I RCCGGY 1167 1171 . . 1167 1172 + BetI* WCCGGW 1167 1171 . . 1167 1172 + AgeI ACCGGT 1167 1171 . . 1168 1171 + HpaII CCGG 1168 1170 . . 1211 1216 + CfrI YGGCCR 1211 1215 . . 1212 1215 + CviJI RGCY 1213 1213 . . 1212 1215 + HaeIII GGCC 1213 1213 . . 1209 1214 - NmeAIII GCCGAG 1189 1187 . . 1215 1218 + FatI CATG 1214 1218 . . 1215 1218 + NlaIII CATG 1218 1214 . . 1215 1218 + CviAII CATG 1215 1217 . . 1216 1220 + TspDTI ATGAA 1231 1229 . . 1227 1238 + PpiI GAACNNNNNCTC 1219 1214 1251 1246 1231 1236 + MmeI TCCRAC 1256 1254 . . 1239 1242 + TspEI AATT 1238 1242 . . 1243 1246 + SetI ASST 1246 1242 . . 1244 1247 + MnlI CCTC 1254 1253 . . 1259 1263 + DdeI CTNAG 1259 1262 . . 1263 1267 + MboII GAAGA 1275 1274 . . 1273 1278 + XbaI TCTAGA 1273 1277 . . 1273 1278 + Hpy178III* TCNNGA 1274 1276 . . 1274 1277 + MaeI CTAG 1274 1276 . . 1284 1287 + TspEI AATT 1283 1287 . . 1286 1289 + MseI TTAA 1286 1288 . . 1291 1294 + TaqI TCGA 1291 1293 . . 1291 1296 + Hpy178III* TCNNGA 1292 1294 . . 1295 1299 + MboII GAAGA 1307 1306 . . 1295 1300 + BbvII* GAAGAC 1302 1306 . . 1307 1311 - HphI GGTGA 1299 1298 . . 1334 1337 + MseI TTAA 1334 1336 . . 1364 1369 - EcoP15I CAGCAG 1336 1338 . . 1373 1378 + ApoI RAATTY 1373 1377 . . 1374 1377 + TspEI AATT 1373 1377 . . 1402 1407 + Hpy166II GTNNAC 1404 1404 . . 1402 1407 + HpaI GTTAAC 1404 1404 . . 1402 1407 + HindII GTYRAC 1404 1404 . . 1403 1406 + MseI TTAA 1403 1405 . . 1417 1420 + MseI TTAA 1417 1419 . . 1425 1428 + SetI ASST 1428 1424 . . 1429 1434 + Hpy166II GTNNAC 1431 1431 . . 1429 1434 + HindII GTYRAC 1431 1431 . . 1435 1438 + TspEI AATT 1434 1438 . . 1459 1471 + PsrI GAACNNNNNNTAC 1451 1446 1483 1478 1464 1469 + ApoI RAATTY 1464 1468 . . 1465 1468 + TspEI AATT 1464 1468 . . 1497 1502 + Hpy166II GTNNAC 1499 1499 . . 1502 1512 + BsiYI* CCNNNNNNNGG 1508 1505 . . 1499 1503 - HphI GGTGA 1491 1490 . . 1507 1511 - BccI CCATC 1501 1502 . . 1512 1517 + BsrDI GCAATG 1519 1517 . . 1517 1521 + FokI GGATG 1530 1534 . . 1517 1521 + BseGI GGATG 1523 1521 . . 1533 1536 + TspEI AATT 1532 1536 . . 1539 1544 + BciVI GTATCC 1550 1549 . . 1543 1546 + AciI CCGC 1543 1545 . . 1542 1547 - EciI GGCGGA 1532 1530 . . 1552 1562 + Hin4I GAYNNNNNVTC 1543 1538 1575 1570 1553 1556 + SetI ASST 1556 1552 . . 1554 1557 + MnlI CCTC 1564 1563 . . 1552 1562 - Hin4I GAYNNNNNVTC 1575 1570 1543 1538 1561 1565 - MboII GAAGA 1553 1552 . . 1565 1570 + StyI CCWWGG 1565 1569 . . 1565 1570 + SecI* CCNNGG 1565 1569 . . 1573 1576 + MseI TTAA 1573 1575 . . 1583 1588 + Hpy178III* TCNNGA 1584 1586 . . 1588 1593 + ApoI RAATTY 1588 1592 . . 1589 1592 + TspEI AATT 1588 1592 . . 1595 1599 + BmgT120I GGNCC 1596 1597 . . 1595 1599 + AvaII GGWCC 1595 1598 . . 1595 1599 + AsuI* GGNCC 1595 1598 . . 1612 1616 - BsmAI GTCTC 1606 1610 . . 1618 1621 + TspEI AATT 1617 1621 . . 1623 1628 + ApoI RAATTY 1623 1627 . . 1624 1627 + TspEI AATT 1623 1627 . . 1663 1667 - BslFI GGGAC 1648 1652 . . 1668 1671 + TspEI AATT 1667 1671 . . 1684 1688 + Hin4II* CCTTC 1694 1693 . . 1687 1691 - HphI GGTGA 1679 1678 . . 1694 1697 + DpnI GATC 1695 1695 . . 1694 1697 + BstKTI GATC 1696 1694 . . 1694 1697 + MboI GATC 1693 1697 . . 1700 1703 + MseI TTAA 1700 1702 . . 1714 1717 + CviJI RGCY 1715 1715 . . 1720 1723 + MseI TTAA 1720 1722 . . 1723 1727 + BceAI ACGGC 1739 1741 . . 1725 1728 + CviJI RGCY 1726 1726 . . 1731 1742 + CspCI CAANNNNNGTGG 1719 1717 1754 1752 1736 1740 + Tsp4CI* ACNGT 1738 1737 . . 1739 1744 + Hpy166II GTNNAC 1741 1741 . . 1747 1752 + ApoI RAATTY 1747 1751 . . 1748 1751 + TspEI AATT 1747 1751 . . 1754 1758 + BmgT120I GGNCC 1755 1756 . . 1754 1758 + AvaII GGWCC 1754 1757 . . 1754 1758 + AsuI* GGNCC 1754 1757 . . 1757 1761 + Hin4II* CCTTC 1767 1766 . . 1760 1763 + TaqI TCGA 1760 1762 . . 1762 1766 + HinfI GANTC 1762 1765 . . 1762 1766 - PleI GAGTC 1756 1757 . . 1762 1766 - MlyI GAGTC 1756 1756 . . 1767 1772 + Hpy178III* TCNNGA 1768 1770 . . 1767 1772 + BspHI TCATGA 1767 1771 . . 1768 1771 + FatI CATG 1767 1771 . . 1768 1771 + NlaIII CATG 1771 1767 . . 1768 1771 + CviAII CATG 1768 1770 . . 1775 1785 + MwoI GCNNNNNNNGC 1781 1778 . . 1775 1785 + BstAPI GCANNNNNTGC 1781 1778 . . 1778 1782 + BsrI ACTGG 1783 1781 . . 1793 1798 + ApoI RAATTY 1793 1797 . . 1794 1797 + TspEI AATT 1793 1797 . . 1811 1814 + MseI TTAA 1811 1813 . . 1823 1827 + MaeIII GTNAC 1822 1827 . . 1828 1832 - TspGWI ACGGA 1818 1816 . . 1839 1842 + SetI ASST 1842 1838 . . 1839 1842 + CviJI RGCY 1840 1840 . . 1839 1842 + AluI AGCT 1840 1840 . . 1844 1849 + Eco31I GGTCTC 1850 1854 . . 1845 1849 + BsmAI GTCTC 1850 1854 . . 1847 1852 + SmlI CTYRAG 1847 1851 . . 1848 1853 + Hpy178III* TCNNGA 1849 1851 . . 1847 1852 - Bce83I* CTTGAG 1832 1830 . . 1856 1861 + Eco31I GGTCTC 1862 1866 . . 1857 1861 + BsmAI GTCTC 1862 1866 . . 1863 1868 + SspI AATATT 1865 1865 . . 1869 1874 + DsaI* CCRYGG 1869 1873 . . 1869 1874 + StyI CCWWGG 1869 1873 . . 1869 1874 + NcoI CCATGG 1869 1873 . . 1869 1874 + SecI* CCNNGG 1869 1873 . . 1870 1873 + FatI CATG 1869 1873 . . 1870 1873 + NlaIII CATG 1873 1869 . . 1870 1873 + CviAII CATG 1870 1872 . . 1877 1880 + TaqI TCGA 1877 1879 . . 1889 1892 + CviRI* TGCA 1890 1890 . . 1892 1897 + ApoI RAATTY 1892 1896 . . 1893 1896 + TspEI AATT 1892 1896 . . 1904 1907 + TaqI TCGA 1904 1906 . . 1912 1917 + AhaIII* TTTAAA 1914 1914 . . 1913 1916 + MseI TTAA 1913 1915 . . 1924 1928 + TfiI GAWTC 1924 1927 . . 1924 1928 + HinfI GANTC 1924 1927 . . 1945 1949 + Tsp4CI* ACNGT 1947 1946 . . 1950 1956 + TspRI CASTGNN 1956 1947 . . 1956 1961 + Hpy166II GTNNAC 1958 1958 . . 1960 1963 + TaiI ACGT 1963 1959 . . 1960 1963 + SetI ASST 1963 1959 . . 1960 1963 + MaeII ACGT 1960 1962 . . 1966 1970 + HinfI GANTC 1966 1969 . . 1969 1973 + Hpy188I TCNGA 1971 1970 . . 1966 1970 - PleI GAGTC 1960 1961 . . 1966 1970 - MlyI GAGTC 1960 1960 . . 1981 1985 + Tsp4CI* ACNGT 1983 1982 . . 1993 1998 + VspI ATTAAT 1994 1996 . . 1994 1997 + MseI TTAA 1994 1996 . . 2004 2009 + ApoI RAATTY 2004 2008 . . 2005 2008 + TspEI AATT 2004 2008 . . 2008 2011 + MseI TTAA 2008 2010 . . 2015 2020 + BalI TGGCCA 2017 2017 . . 2015 2020 + CfrI YGGCCR 2015 2019 . . 2016 2019 + CviJI RGCY 2017 2017 . . 2016 2019 + HaeIII GGCC 2017 2017 . . 2021 2025 + BssKI CCNGG 2020 2025 . . 2021 2025 + BseBI CCWGG 2022 2023 . . 2021 2025 + ScrFI CCNGG 2022 2023 . . 2021 2025 + EcoRII CCWGG 2020 2025 . . 2032 2035 + MseI TTAA 2032 2034 . . 2039 2043 + BccI CCATC 2047 2048 . . 2052 2055 - AciI CCGC 2052 2054 . . 2052 2056 - FauI CCCGC 2045 2047 . . 2085 2089 - TspGWI ACGGA 2075 2073 . . 2092 2097 + Hpy166II GTNNAC 2094 2094 . . 2092 2097 + HpaI GTTAAC 2094 2094 . . 2092 2097 + HindII GTYRAC 2094 2094 . . 2093 2096 + MseI TTAA 2093 2095 . . 2096 2100 + TspGWI ACGGA 2111 2109 . . 2114 2118 + Tsp45I GTSAC 2113 2118 . . 2114 2118 + MaeIII GTNAC 2113 2118 . . 2118 2121 + FatI CATG 2117 2121 . . 2118 2121 + NlaIII CATG 2121 2117 . . 2118 2121 + CviAII CATG 2118 2120 . . 2126 2129 + CviJI RGCY 2127 2127 . . 2138 2142 + Tsp45I GTSAC 2137 2142 . . 2138 2142 + MaeIII GTNAC 2137 2142 . . 2157 2162 - BsrDI GCAATG 2156 2154 . . 2162 2167 + SecI* CCNNGG 2162 2166 . . 2163 2167 + BssKI CCNGG 2162 2167 . . 2163 2167 + BseBI CCWGG 2164 2165 . . 2163 2167 + ScrFI CCNGG 2164 2165 . . 2163 2167 + EcoRII CCWGG 2162 2167 . . 2168 2173 + PsiI TTATAA 2170 2170 . . 2165 2170 - TsoI TARCCA 2155 2153 . . 2188 2191 + DpnI GATC 2189 2189 . . 2188 2191 + BstKTI GATC 2190 2188 . . 2188 2191 + MboI GATC 2187 2191 . . 2190 2195 + Hpy178III* TCNNGA 2191 2193 . . 2192 2195 + TaqI TCGA 2192 2194 . . 2194 2198 - BccI CCATC 2188 2189 . . 2200 2203 + Csp6I GTAC 2200 2202 . . 2200 2203 + RsaI GTAC 2201 2201 . . 2212 2215 + TspEI AATT 2211 2215 . . 2237 2240 + TaqI TCGA 2237 2239 . . 2239 2243 + TfiI GAWTC 2239 2242 . . 2239 2243 + HinfI GANTC 2239 2242 . . 2242 2246 + Hpy188I TCNGA 2244 2243 . . 2258 2261 + TspEI AATT 2257 2261 . . 2266 2269 + TspEI AATT 2265 2269 . . 2274 2278 + SfaNI GCATC 2283 2287 . . 2277 2282 + Hpy178III* TCNNGA 2278 2280 . . 2277 2282 + BspHI TCATGA 2277 2281 . . 2278 2281 + FatI CATG 2277 2281 . . 2278 2281 + NlaIII CATG 2281 2277 . . 2278 2281 + CviAII CATG 2278 2280 . . 2279 2283 + TspDTI ATGAA 2294 2292 . . 2305 2308 + TspEI AATT 2304 2308 . . 2300 2305 - MmeI TCCRAC 2281 2279 . . 2320 2324 - TspDTI ATGAA 2310 2308 . . 2332 2342 + Hin4I GAYNNNNNVTC 2323 2318 2355 2350 2338 2342 + TfiI GAWTC 2338 2341 . . 2338 2342 + HinfI GANTC 2338 2341 . . 2332 2342 - Hin4I GAYNNNNNVTC 2355 2350 2323 2318 2345 2353 + DraIII CACNNNGTG 2350 2347 . . 2347 2350 + MaeI CTAG 2347 2349 . . 2349 2352 + SetI ASST 2352 2348 . . 2357 2361 + Hpy188I TCNGA 2359 2358 . . 2359 2364 + NlaIV GGNNCC 2361 2361 . . 2356 2361 - MmeI TCCRAC 2337 2335 . . 2364 2370 + TspRI CASTGNN 2370 2361 . . 2365 2369 + Tsp4CI* ACNGT 2367 2366 . . 2359 2370 - TstI CACNNNNNNTCC 2383 2378 2351 2346 2383 2387 + Hpy188I TCNGA 2385 2384 . . 2387 2391 + TspDTI ATGAA 2402 2400 . . 2393 2396 + MseI TTAA 2393 2395 . . 2401 2405 - Hin4II* CCTTC 2395 2394 . . 2406 2411 + SspI AATATT 2408 2408 . . 2412 2416 + FokI GGATG 2425 2429 . . 2412 2416 + BseGI GGATG 2418 2416 . . 2416 2419 + CviJI RGCY 2417 2417 . . 2416 2419 + HaeIII GGCC 2417 2417 . . 2413 2417 - BccI CCATC 2407 2408 . . 2418 2422 + BssKI CCNGG 2417 2422 . . 2418 2422 + BseBI CCWGG 2419 2420 . . 2418 2422 + ScrFI CCNGG 2419 2420 . . 2418 2422 + EcoRII CCWGG 2417 2422 . . 2426 2430 + MaeIII GTNAC 2425 2430 . . 2432 2436 + BccI CCATC 2440 2441 . . 2446 2450 + SfaNI GCATC 2455 2459 . . 2447 2451 - FokI GGATG 2433 2437 . . 2447 2451 - BseGI GGATG 2446 2444 . . 2457 2468 + BcgI CGANNNNNNTGC 2446 2444 2480 2478 2466 2469 + CviRI* TGCA 2467 2467 . . 2473 2478 + MfeI CAATTG 2473 2477 . . 2474 2477 + TspEI AATT 2473 2477 . . 2477 2480 + CviRI* TGCA 2478 2478 . . 2484 2488 + Tsp4CI* ACNGT 2486 2485 . . 2492 2495 + MaeI CTAG 2492 2494 . . 2507 2511 + Tsp45I GTSAC 2506 2511 . . 2507 2511 + MaeIII GTNAC 2506 2511 . . 2513 2516 + MaeI CTAG 2513 2515 . . 2538 2542 + Hpy99I CGWCG 2542 2537 . . 2542 2546 + MboII GAAGA 2554 2553 . . 2546 2550 + TspDTI ATGAA 2561 2559 . . 2548 2552 + MboII GAAGA 2560 2559 . . 2551 2555 + MboII GAAGA 2563 2562 . . 2554 2559 + EcoRV GATATC 2556 2556 . . 2566 2571 + ApoI RAATTY 2566 2570 . . 2567 2570 + TspEI AATT 2566 2570 . . 2570 2575 + PsiI TTATAA 2572 2572 . . 2594 2597 + SetI ASST 2597 2593 . . 2594 2597 + CviJI RGCY 2595 2595 . . 2594 2597 + AluI AGCT 2595 2595 . . 2596 2599 + MaeI CTAG 2596 2598 . . 2605 2609 + TspDTI ATGAA 2620 2618 . . 2621 2626 + NspI RCATGY 2625 2621 . . 2622 2625 + FatI CATG 2621 2625 . . 2622 2625 + NlaIII CATG 2625 2621 . . 2622 2625 + CviAII CATG 2622 2624 . . 2628 2639 + BdaI TGANNNNNNTCA 2617 2615 2651 2649 2628 2639 - BdaI TGANNNNNNTCA 2651 2649 2617 2615 2642 2645 + TaiI ACGT 2645 2641 . . 2642 2645 + SetI ASST 2645 2641 . . 2642 2645 + MaeII ACGT 2642 2644 . . 2645 2648 + CviRI* TGCA 2646 2646 . . 2652 2657 - BciVI GTATCC 2646 2645 . . 2665 2668 + DpnI GATC 2666 2666 . . 2665 2668 + BstKTI GATC 2667 2665 . . 2665 2668 + MboI GATC 2664 2668 . . 2674 2678 + TspDTI ATGAA 2689 2687 . . 2683 2686 + MseI TTAA 2683 2685 . . #--------------------------------------- # # # AatII: # [GACGTC] # 644 2047 # # AciI: # [CCGC] # 46 59 190 509 639 1248 # # AclI: # [AACGTT] # 118 466 2107 # # AcyI: # [GRCGYC] # 641 2050 # # AgeI: # [ACCGGT] # 1167 1524 # # AhaIII*: # [TTTAAA] # 356 777 1558 # # AluI: # [AGCT] # 48 96 293 372 755 1127 # # ApoI: # [RAATTY] # 35 46 91 99 112 124 # 124 125 250 278 390 455 # 562 # # AsuI*: # [GGNCC] # 117 159 436 474 568 937 # # AvaII: # [GGWCC] # 159 474 553 568 937 # # AvrII: # [CCTAGG] # 12 2679 # # BalI: # [TGGCCA] # 674 787 1230 # # BamHI: # [GGATCC] # 925 1766 # # BbvII*: # [GAAGAC] # 311 991 1389 # # BccI: # [CCATC] # 33 57 141 180 219 251 # 546 585 679 # # Bce83I*: # [CTTGAG] # 801 859 1031 # # BceAI: # [ACGGC] # 952 1739 # # BcgI: # [CGANNNNNNTGC] # 34 211 2446 # # BciVI: # [GTATCC] # 45 1096 1550 # # BdaI: # [TGANNNNNNTCA] # 0 0 0 0 0 0 # 0 0 34 34 34 34 # 40 54 60 362 2039 # # BetI*: # [WCCGGW] # 223 338 606 1524 # # BinI*: # [GGATC] # 13 920 1758 # # BmgT120I: # [GGNCC] # 117 159 436 475 568 936 # # BmtI: # [GCTAGC] # 625 2066 # # Bpu10I: # [CCTNAGC] # 386 2305 # # BseBI: # [CCWGG] # 142 255 272 853 1169 # # BseGI: # [GGATG] # 28 245 728 795 895 # # BseMII: # [CTCAG] # 87 602 2002 # # BseYI: # [CCCAGC] # 276 2415 # # BsiYI*: # [CCNNNNNNNGG] # 441 1067 1183 # # BslFI: # [GGGAC] # 366 593 689 1043 # # BsmAI: # [GTCTC] # 12 244 648 829 958 # # BsmI: # [GAATGC] # 1128 1563 # # BspCNI: # [CTCAG] # 86 602 2003 # # BspHI: # [TCATGA] # 414 510 1767 # # BspMII*: # [TCCGGA] # 223 606 1862 # # BsrDI: # [GCAATG] # 535 637 1519 # # BsrI: # [ACTGG] # 815 908 968 # # BssKI: # [CCNGG] # 142 255 274 851 1169 # # BstAPI: # [GCANNNNNTGC] # 910 1781 # # BstKTI: # [GATC] # 24 67 155 204 477 494 # 502 768 # # Cac8I: # [GCNNGC] # 105 201 317 2068 # # Cfr10I: # [RCCGGY] # 85 1082 1524 # # CfrI: # [YGGCCR] # 69 357 676 785 804 # # ClaI: # [ATCGAT] # 259 2432 # # Csp6I: # [GTAC] # 261 491 510 1429 # # CspCI: # [CAANNNNNGTGG] # 35 937 1719 # # CviAII: # [CATG] # 69 102 160 248 343 344 # 432 440 553 # # CviJI: # [RGCY] # 5 11 27 37 48 52 # 74 96 110 114 142 151 # 177 178 290 305 372 502 # # CviRI*: # [TGCA] # 11 19 32 45 80 112 # 168 187 205 577 1255 # # DdeI: # [CTNAG] # 73 289 313 584 1432 # # DpnI: # [GATC] # 25 67 154 204 477 494 # 502 768 # # DraII: # [RGGNCCY] # 474 568 1649 # # DraIII: # [CACNNNGTG] # 341 2350 # # DsaI*: # [CCRYGG] # 822 1869 # # EciI: # [GGCGGA] # 1159 1532 # # Eco31I: # [GGTCTC] # 12 829 1850 # # EcoP15I: # [CAGCAG] # 246 1090 1355 # # EcoRII: # [CCWGG] # 142 255 274 851 1169 # # EcoRV: # [GATATC] # 135 2556 # # EcoT22I: # [ATGCAT] # 525 2166 # # Esp3I: # [CGTCTC] # 648 2043 # # FatI: # [CATG] # 70 102 160 248 342 344 # 432 440 553 # # FauI: # [CCCGC] # 46 66 646 1933 # # FokI: # [GGATG] # 8 258 715 815 895 # # GlaI: # [GCGC] # 626 2065 # # GsaI: # [CCCAGC] # 280 2411 # # HaeIII: # [GGCC] # 52 69 274 305 400 787 # 804 # # HgaI: # [GACGC] # 12 304 2375 # # HhaI: # [GCGC] # 627 2064 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 32 32 # 32 336 388 748 1123 # # Hin4II*: # [CCTTC] # 73 296 628 1694 # # Hin6I: # [GCGC] # 625 2066 # # HindII: # [GTYRAC] # 27 597 599 663 805 # # HindIII: # [AAGCTT] # 293 418 1980 # # HinfI: # [GANTC] # 14 42 47 64 99 109 # 148 162 192 273 318 353 # 870 # # HpaI: # [GTTAAC] # 597 690 1404 # # HpaII: # [CCGG] # 86 138 338 606 1523 # # HphI: # [GGTGA] # 29 188 192 292 978 1012 # # Hpy166II: # [GTNNAC] # 27 68 136 189 217 242 # 597 599 616 # # Hpy178III*: # [TCNNGA] # 18 81 87 89 172 184 # 224 292 342 355 413 434 # # Hpy188I: # [TCNGA] # 26 30 115 152 165 273 # 306 784 840 # # Hpy99I: # [CGWCG] # 149 2542 # # Ksp632I*: # [CTCTTC] # 239 2452 # # MaeI: # [CTAG] # 13 21 83 95 143 145 # 157 163 303 495 1073 # # MaeII: # [ACGT] # 49 57 118 132 466 682 # 1187 # # MaeIII: # [GTNAC] # 24 81 81 185 288 291 # 821 920 # # MboI: # [GATC] # 27 67 152 204 477 494 # 502 768 # # MboII: # [GAAGA] # 3 3 6 32 60 128 # 132 246 253 280 547 1001 # # MfeI: # [CAATTG] # 218 973 1500 # # MlyI: # [GAGTC] # 148 186 202 204 350 731 # 870 # # MmeI: # [TCCRAC] # 56 354 458 798 1025 # # MnlI: # [CCTC] # 42 94 105 107 176 295 # 310 435 1127 # # MseI: # [TTAA] # 8 14 14 20 24 45 # 48 61 69 81 88 91 # 102 127 132 143 156 167 # 171 183 290 300 357 # # MwoI: # [GCNNNNNNNGC] # 314 568 899 910 # # NcoI: # [CCATGG] # 822 1869 # # NdeI: # [CATATG] # 629 2062 # # NheI: # [GCTAGC] # 621 2070 # # NlaIII: # [CATG] # 66 102 160 248 344 346 # 432 440 553 # # NlaIV: # [GGNNCC] # 116 201 250 330 476 1318 # # NmeAIII: # [GCCGAG] # 1189 1502 # # NspI: # [RCATGY] # 66 2625 # # PleI: # [GAGTC] # 148 186 201 204 351 731 # 870 # # PpiI: # [GAACNNNNNCTC] # 32 32 142 1045 1440 # # PpuMI: # [RGGWCCY] # 474 568 1649 # # PshAI: # [GACNNNNGTC] # 479 2212 # # PsiI: # [TTATAA] # 119 183 362 402 476 1149 # # PsrI: # [GAACNNNNNNTAC] # 32 1208 1451 # # RsaI: # [GTAC] # 261 490 511 1429 # # ScrFI: # [CCNGG] # 142 255 272 853 1169 # # SecI*: # [CCNNGG] # 12 293 304 529 708 845 # # SetI: # [ASST] # 4 10 15 18 46 47 # 48 48 48 55 57 57 # 71 94 105 121 128 143 # 182 213 245 261 286 389 # # SfaNI: # [GCATC] # 172 236 528 1755 # # SfeI*: # [CTRYAG] # 450 2241 # # SmlI: # [CTYRAG] # 801 844 1046 # # SspI: # [AATATT] # 230 283 370 543 1265 # # StyI: # [CCWWGG] # 12 304 822 1553 # # TaiI: # [ACGT] # 46 57 118 132 469 682 # 1187 # # TaqI: # [TCGA] # 20 27 45 117 118 125 # 141 239 288 454 469 648 # # TaqII: # [CACCCA] # 268 2423 # # TfiI: # [GAWTC] # 14 99 256 315 353 427 # 1227 # # TsoI: # [TARCCA] # 536 2155 # # Tsp45I: # [GTSAC] # 24 185 369 902 1211 # # Tsp4CI*: # [ACNGT] # 9 15 26 33 36 119 # 205 209 256 384 691 708 # # TspDTI: # [ATGAA] # 2 16 59 69 92 159 # 159 269 803 1063 # # TspEI: # [AATT] # 6 6 8 10 13 21 # 29 30 36 39 44 45 # 46 46 56 61 68 80 # 90 93 94 95 95 98 # 99 112 125 155 169 207 # 268 347 # # TspGWI: # [ACGGA] # 36 257 580 1818 # # TspRI: # [CASTGNN] # 321 414 1956 # # TstI: # [CACNNNNNNTCC] # 32 32 308 910 1409 # # VspI: # [ATTAAT] # 697 1994 # # XbaI: # [TCTAGA] # 1273 1418 # # XcmI: # [CCANNNNNNNNNTGG] # 795 1896 # # XhoII: # [RGATCY] # 925 1766 # # ZraI: # [GACGTC] # 642 2049 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 2691 # Reported_sequences: 1 # Reported_hitcount: 551 #---------------------------------------