Restriction Fragments for MNN9/YPL050C



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The currently selected gene/sequence is :

MNN9/YPL050C on chromosome XVI from coordinates 461966 to 460779.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.9243.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 7 + Tsp45I GTSAC 2 7 . . 3 7 + MaeIII GTNAC 2 7 . . 21 24 + Csp6I GTAC 21 23 . . 21 24 + RsaI GTAC 22 22 . . 24 27 + AciI CCGC 24 26 . . 28 32 + DdeI CTNAG 28 31 . . 40 45 + DsaI* CCRYGG 40 44 . . 40 45 + SecI* CCNNGG 40 44 . . 46 51 + Hpy166II GTNNAC 48 48 . . 46 51 + HpaI GTTAAC 48 48 . . 46 51 + HindII GTYRAC 48 48 . . 47 50 + MseI TTAA 47 49 . . 60 63 + SetI ASST 63 59 . . 68 73 + BalI TGGCCA 70 70 . . 68 73 + CfrI YGGCCR 68 72 . . 69 72 + CviJI RGCY 70 70 . . 69 72 + HaeIII GGCC 70 70 . . 90 93 + TspEI AATT 89 93 . . 98 103 + Hpy178III* TCNNGA 99 101 . . 106 109 + DpnI GATC 107 107 . . 106 109 + BstKTI GATC 108 106 . . 106 109 + MboI GATC 105 109 . . 120 124 + BslFI GGGAC 134 138 . . 125 128 + MseI TTAA 125 127 . . 129 134 + BalI TGGCCA 131 131 . . 129 134 + CfrI YGGCCR 129 133 . . 130 133 + CviJI RGCY 131 131 . . 130 133 + HaeIII GGCC 131 131 . . 132 136 - BsrI ACTGG 132 130 . . 155 160 + SduI GDGCHC 159 155 . . 155 160 + BseSI GKGCMC 159 155 . . 188 192 + Hin4II* CCTTC 198 197 . . 191 195 - HphI GGTGA 183 182 . . 215 220 + TatI WGTACW 215 219 . . 215 220 + ScaI AGTACT 217 217 . . 216 219 + Csp6I GTAC 216 218 . . 216 219 + RsaI GTAC 217 217 . . 222 227 + PsiI TTATAA 224 224 . . 236 240 + TauI GCSGC 239 236 . . 236 240 + BlsI GCNGC 238 237 . . 236 240 + BisI GCNGC 237 238 . . 238 241 + CviJI RGCY 239 239 . . 236 239 - AciI CCGC 236 238 . . 253 256 + DpnI GATC 254 254 . . 253 256 + BstKTI GATC 255 253 . . 253 256 + MboI GATC 252 256 . . 256 261 + BsaAI YACGTR 258 258 . . 256 261 + PmaCI CACGTG 258 258 . . 257 260 + TaiI ACGT 260 256 . . 257 260 + SetI ASST 260 256 . . 257 260 + MaeII ACGT 257 259 . . 262 266 + MboII GAAGA 274 273 . . 279 282 + SetI ASST 282 278 . . 277 281 - Hin4II* CCTTC 271 270 . . 288 291 + CviRI* TGCA 289 289 . . 308 311 + TspEI AATT 307 311 . . 311 314 + CviRI* TGCA 312 312 . . 319 322 + TaiI ACGT 322 318 . . 319 322 + SetI ASST 322 318 . . 319 322 + MaeII ACGT 319 321 . . 322 326 + Hpy188I TCNGA 324 323 . . 328 332 + BlsI GCNGC 330 329 . . 328 332 + BisI GCNGC 329 330 . . 328 332 + BbvI GCAGC 340 344 . . 328 332 + TseI GCWGC 328 331 . . 329 334 + PvuII CAGCTG 331 331 . . 329 334 + NspBII* CMGCKG 331 331 . . 330 333 + SetI ASST 333 329 . . 330 333 + CviJI RGCY 331 331 . . 330 333 + AluI AGCT 331 331 . . 354 364 + Hin4I GAYNNNNNVTC 345 340 377 372 360 364 + HinfI GANTC 360 363 . . 360 364 - PleI GAGTC 354 355 . . 360 364 - MlyI GAGTC 354 354 . . 368 371 + CviRI* TGCA 369 369 . . 377 381 - TspDTI ATGAA 367 365 . . 389 393 + BsrI ACTGG 394 392 . . 389 394 + BfiI ACTGGG 399 398 . . 392 396 + BslFI GGGAC 406 410 . . 397 400 + TspEI AATT 396 400 . . 402 406 + BlsI GCNGC 404 403 . . 402 406 + BisI GCNGC 403 404 . . 402 406 + TseI GCWGC 402 405 . . 403 408 + PstI CTGCAG 407 403 . . 403 408 + SfeI* CTRYAG 403 407 . . 404 407 + CviRI* TGCA 405 405 . . 405 409 + BlsI GCNGC 407 406 . . 405 409 + BisI GCNGC 406 407 . . 405 409 + BbvI GCAGC 417 421 . . 405 409 + TseI GCWGC 405 408 . . 402 406 - BbvI GCAGC 389 393 . . 407 410 + SetI ASST 410 406 . . 407 410 + CviJI RGCY 408 408 . . 407 410 + AluI AGCT 408 408 . . 412 415 + TspEI AATT 411 415 . . 418 421 + MnlI CCTC 428 427 . . 418 423 + SecI* CCNNGG 418 422 . . 419 424 + BmeT110I CYCGRG 420 422 . . 419 424 + AvaI CYCGRG 419 423 . . 420 425 + Hpy178III* TCNNGA 421 423 . . 425 428 + TspEI AATT 424 428 . . 434 437 + TspEI AATT 433 437 . . 442 446 - TspDTI ATGAA 432 430 . . 458 466 + AlwNI CAGNNNCTG 463 460 . . 459 462 + CviJI RGCY 460 460 . . 461 475 + XcmI CCANNNNNNNNNTGG 468 467 . . 462 468 + TspRI CASTGNN 468 459 . . 463 467 + BsrI ACTGG 468 466 . . 466 470 + HphI GGTGA 478 477 . . 467 471 + Tsp45I GTSAC 466 471 . . 467 471 + MaeIII GTNAC 466 471 . . 474 477 + CviJI RGCY 475 475 . . 474 477 + HaeIII GGCC 475 475 . . 478 481 + MseI TTAA 478 480 . . 485 488 + TspEI AATT 484 488 . . 492 497 + BsmI GAATGC 498 496 . . 500 503 + MseI TTAA 500 502 . . 506 509 + SetI ASST 509 505 . . 514 518 + TspGWI ACGGA 529 527 . . 543 546 + TspEI AATT 542 546 . . 561 566 + EcoRI GAATTC 561 565 . . 561 566 + ApoI RAATTY 561 565 . . 562 565 + TspEI AATT 561 565 . . 585 589 - BccI CCATC 579 580 . . 591 595 - Hin4II* CCTTC 585 584 . . 597 607 + FalI AAGNNNNNCTT 588 583 620 615 597 607 - FalI AAGNNNNNCTT 620 615 588 583 627 630 + TaiI ACGT 630 626 . . 627 630 + SetI ASST 630 626 . . 627 630 + MaeII ACGT 627 629 . . 629 634 + BsgI GTGCAG 650 648 . . 630 633 + CviRI* TGCA 631 631 . . 631 641 + MwoI GCNNNNNNNGC 637 634 . . 631 635 + BlsI GCNGC 633 632 . . 631 635 + BisI GCNGC 632 633 . . 631 635 + BbvI GCAGC 643 647 . . 631 635 + TseI GCWGC 631 634 . . 626 631 - BtrI CACGTC 628 628 . . 634 639 + BsrDI GCAATG 641 639 . . 639 642 + CviJI RGCY 640 640 . . 646 649 + Hin6I GCGC 646 648 . . 646 649 + GlaI GCGC 647 647 . . 646 651 + Cac8I GCNNGC 648 648 . . 646 651 + BsePI GCGCGC 646 650 . . 646 649 + HhaI GCGC 648 646 . . 647 650 + FnuDII* CGCG 648 648 . . 648 651 + Hin6I GCGC 648 650 . . 648 651 + GlaI GCGC 649 649 . . 648 651 + HhaI GCGC 650 648 . . 650 655 + BsrDI GCAATG 657 655 . . 653 657 + TspDTI ATGAA 668 666 . . 656 659 + TspEI AATT 655 659 . . 675 681 + PpuMI RGGWCCY 676 679 . . 675 681 + DraII RGGNCCY 676 679 . . 676 680 + BmgT120I GGNCC 677 678 . . 676 680 + AvaII GGWCC 676 679 . . 676 680 + AsuI* GGNCC 676 679 . . 678 681 + SetI ASST 681 677 . . 679 682 + MnlI CCTC 689 688 . . 691 696 - TaqII CACCCA 681 679 . . 701 704 + CviJI RGCY 702 702 . . 703 706 + MaeI CTAG 703 705 . . 700 705 - TsoI TARCCA 690 688 . . 706 710 - SfaNI GCATC 696 700 . . 721 725 - BsmAI GTCTC 715 719 . . 730 734 + BccI CCATC 738 739 . . 736 739 + MseI TTAA 736 738 . . 738 741 + TspEI AATT 737 741 . . 741 746 + Hpy178III* TCNNGA 742 744 . . 747 750 + FatI CATG 746 750 . . 747 750 + NlaIII CATG 750 746 . . 747 750 + CviAII CATG 747 749 . . 765 768 + SetI ASST 768 764 . . 765 768 + CviJI RGCY 766 766 . . 765 768 + AluI AGCT 766 766 . . 766 776 + MwoI GCNNNNNNNGC 772 769 . . 771 775 + DdeI CTNAG 771 774 . . 774 777 + SetI ASST 777 773 . . 774 777 + CviJI RGCY 775 775 . . 774 777 + AluI AGCT 775 775 . . 775 779 + BlsI GCNGC 777 776 . . 775 779 + BisI GCNGC 776 777 . . 775 779 + TseI GCWGC 775 778 . . 777 780 + CviRI* TGCA 778 778 . . 775 779 - BbvI GCAGC 762 766 . . 803 807 + TspDTI ATGAA 818 816 . . 805 809 + MboII GAAGA 817 816 . . 810 814 + MboII GAAGA 822 821 . . 805 810 - Ksp632I* CTCTTC 800 803 . . 820 824 + BccI CCATC 828 829 . . 827 831 + Hpy188I TCNGA 829 828 . . 848 852 + BsrI ACTGG 853 851 . . 860 864 + Tsp45I GTSAC 859 864 . . 860 864 + MaeIII GTNAC 859 864 . . 865 870 + Cfr10I RCCGGY 865 869 . . 865 870 + BetI* WCCGGW 865 869 . . 865 870 + AgeI ACCGGT 865 869 . . 866 869 + HpaII CCGG 866 868 . . 879 882 + CviJI RGCY 880 880 . . 881 884 + MnlI CCTC 891 890 . . 884 888 + DdeI CTNAG 884 887 . . 884 888 + BspCNI CTCAG 897 895 . . 884 888 + BseMII CTCAG 898 896 . . 885 889 + Hpy188I TCNGA 887 886 . . 888 892 - BccI CCATC 882 883 . . 892 896 + HphI GGTGA 904 903 . . 890 895 - TaqII CACCCA 880 878 . . 911 914 + TaqI TCGA 911 913 . . 912 923 + BcgI CGANNNNNNTGC 901 899 935 933 913 916 - MnlI CCTC 906 905 . . 921 924 + CviRI* TGCA 922 922 . . 922 932 + MwoI GCNNNNNNNGC 928 925 . . 922 932 + BstAPI GCANNNNNTGC 928 925 . . 927 930 + TspEI AATT 926 930 . . 932 942 + BsiYI* CCNNNNNNNGG 938 935 . . 941 944 + CviJI RGCY 942 942 . . 941 944 + HaeIII GGCC 942 942 . . 945 950 + VspI ATTAAT 946 948 . . 946 949 + MseI TTAA 946 948 . . 951 954 + CviJI RGCY 952 952 . . 964 968 - SfaNI GCATC 954 958 . . 976 979 + Csp6I GTAC 976 978 . . 976 979 + RsaI GTAC 977 977 . . 978 984 + SexAI ACCWGGT 978 983 . . 979 983 + BssKI CCNGG 978 983 . . 979 983 + BseBI CCWGG 980 981 . . 979 983 + ScrFI CCNGG 980 981 . . 979 983 + EcoRII CCWGG 978 983 . . 981 984 + SetI ASST 984 980 . . 982 986 + HphI GGTGA 994 993 . . 985 989 + MboII GAAGA 997 996 . . 985 990 - Ksp632I* CTCTTC 980 983 . . 993 998 + HaeII RGCGCY 997 993 . . 994 997 + Hin6I GCGC 994 996 . . 994 997 + GlaI GCGC 995 995 . . 990 994 - BccI CCATC 984 985 . . 994 997 + HhaI GCGC 996 994 . . 999 1003 + FokI GGATG 1012 1016 . . 999 1003 + BseGI GGATG 1005 1003 . . 1000 1004 - BccI CCATC 994 995 . . 1015 1018 + CviJI RGCY 1016 1016 . . 1013 1016 - MnlI CCTC 1006 1005 . . 1018 1023 + TatI WGTACW 1018 1022 . . 1019 1022 + Csp6I GTAC 1019 1021 . . 1019 1022 + RsaI GTAC 1020 1020 . . 1039 1044 + Hpy166II GTNNAC 1041 1041 . . 1049 1053 + Tsp4CI* ACNGT 1051 1050 . . 1046 1050 - BsmAI GTCTC 1040 1044 . . 1046 1051 - Esp3I CGTCTC 1040 1044 . . 1051 1056 + NlaIV GGNNCC 1053 1053 . . 1051 1056 + HgiCI* GGYRCC 1051 1055 . . 1056 1059 + FatI CATG 1055 1059 . . 1056 1059 + NlaIII CATG 1059 1055 . . 1056 1059 + CviAII CATG 1056 1058 . . 1066 1069 + TspEI AATT 1065 1069 . . 1098 1101 + SetI ASST 1101 1097 . . 1096 1100 - Hin4II* CCTTC 1090 1089 . . 1105 1115 + MwoI GCNNNNNNNGC 1111 1108 . . 1106 1110 + DdeI CTNAG 1106 1109 . . 1110 1114 - BccI CCATC 1104 1105 . . 1116 1120 + MboII GAAGA 1128 1127 . . 1116 1121 - Ksp632I* CTCTTC 1111 1114 . . 1123 1126 + MseI TTAA 1123 1125 . . 1141 1144 + CviJI RGCY 1142 1142 . . 1171 1174 - MnlI CCTC 1164 1163 . . 1174 1178 + MboII GAAGA 1186 1185 . . 1174 1179 - Ksp632I* CTCTTC 1169 1172 . . #--------------------------------------- # # # AciI: # [CCGC] # 24 212 952 # # AgeI: # [ACCGGT] # 323 865 # # AluI: # [AGCT] # 9 77 331 358 413 # # AlwNI: # [CAGNNNCTG] # 463 725 # # ApoI: # [RAATTY] # 561 627 # # AsuI*: # [GGNCC] # 512 676 # # AvaI: # [CYCGRG] # 419 769 # # AvaII: # [GGWCC] # 512 676 # # BalI: # [TGGCCA] # 61 70 1057 # # BbvI: # [GCAGC] # 28 49 119 226 340 426 # # BccI: # [CCATC] # 10 54 84 90 102 110 # 159 579 # # BcgI: # [CGANNNNNNTGC] # 34 253 901 # # BetI*: # [WCCGGW] # 323 865 # # BfiI: # [ACTGGG] # 399 789 # # BisI: # [GCNGC] # 3 74 92 144 226 237 # 412 # # BlsI: # [GCNGC] # 3 74 92 144 226 238 # 411 # # BmeT110I: # [CYCGRG] # 420 768 # # BmgT120I: # [GGNCC] # 511 677 # # BsaAI: # [YACGTR] # 258 930 # # BseBI: # [CCWGG] # 208 980 # # BseGI: # [GGATG] # 183 1005 # # BseMII: # [CTCAG] # 290 898 # # BsePI: # [GCGCGC] # 542 646 # # BseSI: # [GKGCMC] # 159 1029 # # BsgI: # [GTGCAG] # 538 650 # # BsiYI*: # [CCNNNNNNNGG] # 250 938 # # BslFI: # [GGGAC] # 134 272 782 # # BsmAI: # [GTCTC] # 148 325 715 # # BsmI: # [GAATGC] # 498 690 # # BspCNI: # [CTCAG] # 291 897 # # BsrDI: # [GCAATG] # 16 531 641 # # BsrI: # [ACTGG] # 74 132 262 335 385 # # BssKI: # [CCNGG] # 210 978 # # BstAPI: # [GCANNNNNTGC] # 260 928 # # BstKTI: # [GATC] # 108 147 933 # # BtrI: # [CACGTC] # 560 628 # # Cac8I: # [GCNNGC] # 540 648 # # Cfr10I: # [RCCGGY] # 323 865 # # CfrI: # [YGGCCR] # 61 68 1059 # # Csp6I: # [GTAC] # 21 43 169 195 760 # # CviAII: # [CATG] # 132 309 747 # # CviJI: # [RGCY] # 9 10 15 46 52 61 # 62 62 64 64 70 77 # 92 105 108 126 165 # # CviRI*: # [TGCA] # 23 36 57 144 147 226 # 266 289 # # DdeI: # [CTNAG] # 28 82 113 222 743 # # DpnI: # [GATC] # 107 147 934 # # DraII: # [RGGNCCY] # 512 676 # # DsaI*: # [CCRYGG] # 40 1148 # # EcoRI: # [GAATTC] # 561 627 # # EcoRII: # [CCWGG] # 210 978 # # Esp3I: # [CGTCTC] # 148 1040 # # FalI: # [AAGNNNNNCTT] # 0 0 32 568 588 # # FatI: # [CATG] # 133 309 746 # # FnuDII*: # [CGCG] # 540 648 # # FokI: # [GGATG] # 176 1012 # # GlaI: # [GCGC] # 2 193 346 647 # # HaeII: # [RGCGCY] # 191 997 # # HaeIII: # [GGCC] # 61 70 246 344 467 # # HgiCI*: # [GGYRCC] # 137 1051 # # HhaI: # [GCGC] # 2 192 346 648 # # Hin4I: # [GAYNNNNNVTC] # 32 345 811 # # Hin4II*: # [CCTTC] # 73 98 198 314 505 # # Hin6I: # [GCGC] # 2 194 346 646 # # HindII: # [GTYRAC] # 48 1140 # # HinfI: # [GANTC] # 360 828 # # HpaI: # [GTTAAC] # 48 1140 # # HpaII: # [CCGG] # 322 866 # # HphI: # [GGTGA] # 90 183 194 295 426 # # Hpy166II: # [GTNNAC] # 48 147 993 # # Hpy178III*: # [TCNNGA] # 99 321 322 446 # # Hpy188I: # [TCNGA] # 58 301 324 505 # # Ksp632I*: # [CTCTTC] # 19 58 131 180 800 # # MaeI: # [CTAG] # 485 703 # # MaeII: # [ACGT] # 62 257 308 561 # # MaeIII: # [GTNAC] # 2 329 393 464 # # MboI: # [GATC] # 105 147 936 # # MboII: # [GAAGA] # 2 5 58 131 175 274 # 543 # # MlyI: # [GAGTC] # 354 834 # # MnlI: # [CCTC] # 15 24 100 158 202 261 # 428 # # MseI: # [TTAA] # 22 47 65 78 177 210 # 236 353 # # MwoI: # [GCNNNNNNNGC] # 77 135 156 183 637 # # NlaIII: # [CATG] # 129 309 750 # # NlaIV: # [GGNNCC] # 135 1053 # # NspBII*: # [CMGCKG] # 331 857 # # PleI: # [GAGTC] # 354 834 # # PmaCI: # [CACGTG] # 258 930 # # PpuMI: # [RGGWCCY] # 512 676 # # PsiI: # [TTATAA] # 224 964 # # PstI: # [CTGCAG] # 407 781 # # PvuII: # [CAGCTG] # 331 857 # # RsaI: # [GTAC] # 22 43 168 195 760 # # ScaI: # [AGTACT] # 217 971 # # ScrFI: # [CCNGG] # 208 980 # # SduI: # [GDGCHC] # 159 1029 # # SecI*: # [CCNNGG] # 40 378 770 # # SetI: # [ASST] # 9 11 22 40 51 63 # 77 87 87 99 117 121 # 197 207 # # SexAI: # [ACCWGGT] # 210 978 # # SfaNI: # [GCATC] # 234 258 696 # # SfeI*: # [CTRYAG] # 403 785 # # TaiI: # [ACGT] # 62 260 308 558 # # TaqI: # [TCGA] # 277 911 # # TaqII: # [CACCCA] # 199 308 681 # # TatI: # [WGTACW] # 170 215 803 # # TauI: # [GCSGC] # 239 949 # # TseI: # [GCWGC] # 3 74 144 226 328 413 # # TsoI: # [TARCCA] # 498 690 # # Tsp45I: # [GTSAC] # 2 329 393 464 # # Tsp4CI*: # [ACNGT] # 137 1051 # # TspDTI: # [ATGAA] # 65 150 236 367 370 # # TspEI: # [AATT] # 9 13 15 19 51 58 # 82 89 89 94 123 139 # 189 218 # # TspGWI: # [ACGGA] # 529 659 # # TspRI: # [CASTGNN] # 468 720 # # VspI: # [ATTAAT] # 242 946 # # XcmI: # [CCANNNNNNNNNTGG] # 468 720 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1188 # Reported_sequences: 1 # Reported_hitcount: 267 #---------------------------------------