Restriction Fragments for RRP12/YPL012W



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The currently selected gene/sequence is :

RRP12/YPL012W on chromosome XVI from coordinates 529723 to 533409.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.22781.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 7 + BinI* GGATC 11 12 . . 4 7 + DpnI GATC 5 5 . . 4 7 + BstKTI GATC 6 4 . . 4 7 + MboI GATC 3 7 . . 6 11 + Hpy178III* TCNNGA 7 9 . . 32 35 + SetI ASST 35 31 . . 32 35 + CviJI RGCY 33 33 . . 32 35 + AluI AGCT 33 33 . . 34 39 + Eco57MI CTGRAG 55 53 . . 34 39 + GsuI CTGGAG 55 53 . . 37 40 - MnlI CCTC 30 29 . . 44 47 + SetI ASST 47 43 . . 44 47 + CviJI RGCY 45 45 . . 44 47 + AluI AGCT 45 45 . . 45 50 + Cac8I GCNNGC 47 47 . . 48 51 + CviJI RGCY 49 49 . . 47 52 - TsoI TARCCA 37 35 . . 53 58 + ApoI RAATTY 53 57 . . 54 57 + TspEI AATT 53 57 . . 67 72 + Hpy166II GTNNAC 69 69 . . 77 80 + TspEI AATT 76 80 . . 84 88 + TfiI GAWTC 84 87 . . 84 88 + HinfI GANTC 84 87 . . 105 110 + SspI AATATT 107 107 . . 109 112 + MseI TTAA 109 111 . . 115 119 - BceAI ACGGC 100 102 . . 121 124 - MnlI CCTC 114 113 . . 127 132 + SspI AATATT 129 129 . . 132 135 + CviRI* TGCA 133 133 . . 133 143 + MwoI GCNNNNNNNGC 139 136 . . 135 138 + SetI ASST 138 134 . . 133 138 - BspMI ACCTGC 124 128 . . 141 144 + SetI ASST 144 140 . . 141 144 + CviJI RGCY 142 142 . . 141 144 + AluI AGCT 142 142 . . 172 175 + TspEI AATT 171 175 . . 192 196 + Tsp45I GTSAC 191 196 . . 192 196 + MaeIII GTNAC 191 196 . . 201 205 + BinI* GGATC 209 210 . . 202 205 + DpnI GATC 203 203 . . 202 205 + BstKTI GATC 204 202 . . 202 205 + MboI GATC 201 205 . . 211 216 + Hpy166II GTNNAC 213 213 . . 211 216 + AccI GTMKAC 212 214 . . 217 221 + BccI CCATC 225 226 . . 225 230 + Hpy178III* TCNNGA 226 228 . . 225 230 + BspHI TCATGA 225 229 . . 226 229 + FatI CATG 225 229 . . 226 229 + NlaIII CATG 229 225 . . 226 229 + CviAII CATG 226 228 . . 227 231 + TspDTI ATGAA 242 240 . . 244 249 + MfeI CAATTG 244 248 . . 245 248 + TspEI AATT 244 248 . . 249 252 + CviJI RGCY 250 250 . . 254 257 + MnlI CCTC 264 263 . . 270 275 + XbaI TCTAGA 270 274 . . 270 275 + Hpy178III* TCNNGA 271 273 . . 267 271 - MboII GAAGA 259 258 . . 271 274 + MaeI CTAG 271 273 . . 273 278 + XhoII RGATCY 273 277 . . 273 278 + BglII AGATCT 273 277 . . 274 277 + DpnI GATC 275 275 . . 274 277 + BstKTI GATC 276 274 . . 274 277 + MboI GATC 273 277 . . 276 280 + Hpy188I TCNGA 278 277 . . 284 288 - TspDTI ATGAA 274 272 . . 295 301 + SauI* CCTNAGG 296 299 . . 296 300 + DdeI CTNAG 296 299 . . 299 302 + SetI ASST 302 298 . . 307 310 + MseI TTAA 307 309 . . 311 314 + DpnI GATC 312 312 . . 311 314 + BstKTI GATC 313 311 . . 311 314 + MboI GATC 310 314 . . 313 316 + TaqI TCGA 313 315 . . 322 326 + Hpy188I TCNGA 324 323 . . 339 342 + TspEI AATT 338 342 . . 347 350 + FatI CATG 346 350 . . 347 350 + NlaIII CATG 350 346 . . 347 350 + CviAII CATG 347 349 . . 354 360 + TspRI CASTGNN 360 351 . . 356 361 + PstI CTGCAG 360 356 . . 356 361 + SfeI* CTRYAG 356 360 . . 357 360 + CviRI* TGCA 358 358 . . 358 368 + MwoI GCNNNNNNNGC 364 361 . . 354 359 - BtsI GCAGTG 353 351 . . 366 369 + CviJI RGCY 367 367 . . 376 379 + MnlI CCTC 386 385 . . 381 384 + TspEI AATT 380 384 . . 387 390 + SetI ASST 390 386 . . 387 390 + CviJI RGCY 388 388 . . 387 390 + AluI AGCT 388 388 . . 388 392 + BlsI GCNGC 390 389 . . 388 392 + BisI GCNGC 389 390 . . 388 392 + TseI GCWGC 388 391 . . 388 392 - BbvI GCAGC 375 379 . . 406 410 + TfiI GAWTC 406 409 . . 406 410 + HinfI GANTC 406 409 . . 406 415 + XmnI GAANNNNTTC 410 410 . . 410 415 + Ksp632I* CTCTTC 416 419 . . 411 415 - MboII GAAGA 403 402 . . 421 431 + MwoI GCNNNNNNNGC 427 424 . . 422 427 + SmlI CTYRAG 422 426 . . 423 428 + Hpy178III* TCNNGA 424 426 . . 422 427 - Bce83I* CTTGAG 407 405 . . 429 432 + CviRI* TGCA 430 430 . . 427 431 - SfaNI GCATC 417 421 . . 435 438 + CviJI RGCY 436 436 . . 437 441 + BssKI CCNGG 436 441 . . 437 441 + BseBI CCWGG 438 439 . . 437 441 + ScrFI CCNGG 438 439 . . 437 441 + EcoRII CCWGG 436 441 . . 454 457 + DpnI GATC 455 455 . . 454 457 + BstKTI GATC 456 454 . . 454 457 + MboI GATC 453 457 . . 461 464 + TaiI ACGT 464 460 . . 461 464 + SetI ASST 464 460 . . 461 464 + MaeII ACGT 461 463 . . 463 467 + Tsp45I GTSAC 462 467 . . 463 467 + MaeIII GTNAC 462 467 . . 469 479 + BsiYI* CCNNNNNNNGG 475 472 . . 477 480 + SetI ASST 480 476 . . 476 479 - MnlI CCTC 469 468 . . 482 485 + CviRI* TGCA 483 483 . . 488 493 + Hpy166II GTNNAC 490 490 . . 488 493 + BssNAI GTATAC 490 490 . . 488 493 + AccI GTMKAC 489 491 . . 534 539 + HaeII RGCGCY 538 534 . . 534 539 + Eco47III AGCGCT 536 536 . . 535 538 + Hin6I GCGC 535 537 . . 535 538 + GlaI GCGC 536 536 . . 535 545 + MwoI GCNNNNNNNGC 541 538 . . 535 538 + HhaI GCGC 537 535 . . 538 541 + MaeI CTAG 538 540 . . 543 546 + CviRI* TGCA 544 544 . . 544 554 + MwoI GCNNNNNNNGC 550 547 . . 544 554 + BstAPI GCANNNNNTGC 550 547 . . 541 545 - SfaNI GCATC 531 535 . . 550 553 + FatI CATG 549 553 . . 550 553 + NlaIII CATG 553 549 . . 550 553 + CviAII CATG 550 552 . . 548 552 - TspDTI ATGAA 538 536 . . 553 556 - AciI CCGC 553 555 . . 562 565 + MseI TTAA 562 564 . . 568 571 + MnlI CCTC 578 577 . . 576 579 + CviRI* TGCA 577 577 . . 577 587 + MwoI GCNNNNNNNGC 583 580 . . 577 587 + BstAPI GCANNNNNTGC 583 580 . . 584 589 + PstI CTGCAG 588 584 . . 584 589 + SfeI* CTRYAG 584 588 . . 585 588 + CviRI* TGCA 586 586 . . 591 596 + NspI RCATGY 595 591 . . 592 595 + FatI CATG 591 595 . . 592 595 + NlaIII CATG 595 591 . . 592 595 + CviAII CATG 592 594 . . 598 602 + TauI GCSGC 601 598 . . 598 602 + BlsI GCNGC 600 599 . . 598 602 + BisI GCNGC 599 600 . . 598 601 - AciI CCGC 598 600 . . 623 627 + Tsp45I GTSAC 622 627 . . 623 627 + MaeIII GTNAC 622 627 . . 639 642 + SetI ASST 642 638 . . 637 642 - BspMI ACCTGC 628 632 . . 645 650 + AhaIII* TTTAAA 647 647 . . 646 649 + MseI TTAA 646 648 . . 652 655 + DpnI GATC 653 653 . . 652 655 + BstKTI GATC 654 652 . . 652 655 + MboI GATC 651 655 . . 656 661 + Hpy178III* TCNNGA 657 659 . . 658 661 + TaqI TCGA 658 660 . . 660 665 + ApoI RAATTY 660 664 . . 661 664 + TspEI AATT 660 664 . . 676 681 + MfeI CAATTG 676 680 . . 677 680 + TspEI AATT 676 680 . . 700 704 + MboII GAAGA 712 711 . . 700 705 + BbvII* GAAGAC 707 711 . . 707 710 + MseI TTAA 707 709 . . 713 716 + MaeI CTAG 713 715 . . 720 723 + FatI CATG 719 723 . . 720 723 + NlaIII CATG 723 719 . . 720 723 + CviAII CATG 720 722 . . 723 726 + Hin6I GCGC 723 725 . . 723 726 + GlaI GCGC 724 724 . . 723 726 + HhaI GCGC 725 723 . . 730 734 + DdeI CTNAG 730 733 . . 733 736 + SetI ASST 736 732 . . 743 748 - Eco57MI CTGRAG 728 726 . . 743 748 - Eco57I CTGAAG 728 726 . . 750 755 + Esp3I CGTCTC 756 760 . . 751 755 + BsmAI GTCTC 756 760 . . 757 761 + BsrI ACTGG 762 760 . . 762 766 + Tsp4CI* ACNGT 764 763 . . 763 769 + TspRI CASTGNN 769 760 . . 766 771 + BalI TGGCCA 768 768 . . 766 771 + CfrI YGGCCR 766 770 . . 767 770 + CviJI RGCY 768 768 . . 767 770 + HaeIII GGCC 768 768 . . 769 773 + BccI CCATC 777 778 . . 772 776 - MboII GAAGA 764 763 . . 780 790 + Hin4I GAYNNNNNVTC 771 766 803 798 785 788 + CviJI RGCY 786 786 . . 787 790 + MnlI CCTC 797 796 . . 798 803 + TatI WGTACW 798 802 . . 799 802 + Csp6I GTAC 799 801 . . 799 802 + RsaI GTAC 800 800 . . 809 813 + PleI GAGTC 817 818 . . 809 813 + MlyI GAGTC 818 818 . . 809 813 + HinfI GANTC 809 812 . . 811 815 + Tsp45I GTSAC 810 815 . . 811 815 + MaeIII GTNAC 810 815 . . 812 816 - HphI GGTGA 804 803 . . 820 823 + SetI ASST 823 819 . . 820 823 + CviJI RGCY 821 821 . . 820 823 + AluI AGCT 821 821 . . 822 826 + DdeI CTNAG 822 825 . . 822 826 + BspCNI CTCAG 835 833 . . 822 826 + BseMII CTCAG 836 834 . . 823 827 + Hpy188I TCNGA 825 824 . . 843 846 + CviRI* TGCA 844 844 . . 844 848 + SfaNI GCATC 853 857 . . 848 857 + MslI CAYNNNNRTG 852 852 . . 866 871 + NspI RCATGY 870 866 . . 867 870 + FatI CATG 866 870 . . 867 870 + NlaIII CATG 870 866 . . 867 870 + CviAII CATG 867 869 . . 901 906 + Hpy178III* TCNNGA 902 904 . . 898 902 - MboII GAAGA 890 889 . . 909 912 + CviJI RGCY 910 910 . . 921 924 + Csp6I GTAC 921 923 . . 921 924 + RsaI GTAC 922 922 . . 923 926 + SetI ASST 926 922 . . 925 929 + DdeI CTNAG 925 928 . . 929 933 + PleI GAGTC 937 938 . . 929 933 + MlyI GAGTC 938 938 . . 929 933 + HinfI GANTC 929 932 . . 936 941 - BciVI GTATCC 930 929 . . 948 951 + CviRI* TGCA 949 949 . . 952 955 + MseI TTAA 952 954 . . 959 962 + MnlI CCTC 969 968 . . 968 971 + TaqI TCGA 968 970 . . 979 982 + MseI TTAA 979 981 . . 980 985 + TsoI TARCCA 996 994 . . 983 993 + BsiYI* CCNNNNNNNGG 989 986 . . 983 993 + PflMI CCANNNNNTGG 989 986 . . 988 994 + PfoI TCCNGGA 988 993 . . 989 993 + BssKI CCNGG 988 993 . . 989 993 + BseBI CCWGG 990 991 . . 989 993 + ScrFI CCNGG 990 991 . . 989 993 + EcoRII CCWGG 988 993 . . 1007 1010 + MseI TTAA 1007 1009 . . 1017 1022 + Hpy166II GTNNAC 1019 1019 . . 1040 1046 + TspRI CASTGNN 1046 1037 . . 1047 1052 + HaeII RGCGCY 1051 1047 . . 1047 1052 + Eco47III AGCGCT 1049 1049 . . 1048 1051 + Hin6I GCGC 1048 1050 . . 1048 1051 + GlaI GCGC 1049 1049 . . 1048 1051 + HhaI GCGC 1050 1048 . . 1052 1057 + SnaBI TACGTA 1054 1054 . . 1052 1057 + BsaAI YACGTR 1054 1054 . . 1053 1056 + TaiI ACGT 1056 1052 . . 1053 1056 + SetI ASST 1056 1052 . . 1053 1056 + MaeII ACGT 1053 1055 . . 1058 1063 + ApoI RAATTY 1058 1062 . . 1059 1062 + TspEI AATT 1058 1062 . . 1063 1067 + BssKI CCNGG 1062 1067 . . 1063 1067 + BseBI CCWGG 1064 1065 . . 1063 1067 + ScrFI CCNGG 1064 1065 . . 1063 1067 + EcoRII CCWGG 1062 1067 . . 1083 1088 + Hpy166II GTNNAC 1085 1085 . . 1083 1088 + SduI GDGCHC 1087 1083 . . 1083 1088 + BseSI GKGCMC 1087 1083 . . 1083 1088 + ApaLI GTGCAC 1083 1087 . . 1083 1088 + HgiAI* GWGCWC 1087 1083 . . 1084 1087 + CviRI* TGCA 1085 1085 . . 1095 1098 + SetI ASST 1098 1094 . . 1095 1098 + CviJI RGCY 1096 1096 . . 1095 1098 + AluI AGCT 1096 1096 . . 1097 1100 + MaeI CTAG 1097 1099 . . 1107 1112 + Hpy178III* TCNNGA 1108 1110 . . 1106 1111 - Eco57MI CTGRAG 1091 1089 . . 1106 1111 - GsuI CTGGAG 1091 1089 . . 1114 1119 + Hpy166II GTNNAC 1116 1116 . . 1116 1119 + Csp6I GTAC 1116 1118 . . 1116 1119 + RsaI GTAC 1117 1117 . . 1122 1125 + SetI ASST 1125 1121 . . 1122 1125 + CviJI RGCY 1123 1123 . . 1122 1125 + AluI AGCT 1123 1123 . . 1123 1127 + BlsI GCNGC 1125 1124 . . 1123 1127 + BisI GCNGC 1124 1125 . . 1123 1127 + TseI GCWGC 1123 1126 . . 1127 1130 + MnlI CCTC 1137 1136 . . 1123 1127 - BbvI GCAGC 1110 1114 . . 1153 1157 + Hpy188I TCNGA 1155 1154 . . 1154 1158 + DdeI CTNAG 1154 1157 . . 1156 1160 + PleI GAGTC 1164 1165 . . 1156 1160 + MlyI GAGTC 1165 1165 . . 1156 1160 + HinfI GANTC 1156 1159 . . 1154 1158 - BspCNI CTCAG 1146 1144 . . 1154 1158 - BseMII CTCAG 1145 1143 . . 1163 1166 + MseI TTAA 1163 1165 . . 1182 1187 + Hpy166II GTNNAC 1184 1184 . . 1182 1187 + BssNAI GTATAC 1184 1184 . . 1182 1187 + AccI GTMKAC 1183 1185 . . 1187 1195 + DraIII CACNNNGTG 1192 1189 . . 1197 1201 + FokI GGATG 1210 1214 . . 1197 1201 + BseGI GGATG 1203 1201 . . 1203 1212 + XmnI GAANNNNTTC 1207 1207 . . 1217 1220 + TaiI ACGT 1220 1216 . . 1217 1220 + SetI ASST 1220 1216 . . 1217 1220 + MaeII ACGT 1217 1219 . . 1223 1227 + TspDTI ATGAA 1238 1236 . . 1239 1242 + TspEI AATT 1238 1242 . . 1256 1259 + TaqI TCGA 1256 1258 . . 1258 1268 + Hin4I GAYNNNNNVTC 1249 1244 1281 1276 1258 1268 - Hin4I GAYNNNNNVTC 1281 1276 1249 1244 1288 1293 + Hpy178III* TCNNGA 1289 1291 . . 1295 1300 + ApoI RAATTY 1295 1299 . . 1296 1299 + TspEI AATT 1295 1299 . . 1300 1305 + SmlI CTYRAG 1300 1304 . . 1300 1305 + AflII CTTAAG 1300 1304 . . 1301 1304 + MseI TTAA 1301 1303 . . 1315 1319 + BlsI GCNGC 1317 1316 . . 1315 1319 + BisI GCNGC 1316 1317 . . 1315 1319 + BbvI GCAGC 1327 1331 . . 1315 1319 + TseI GCWGC 1315 1318 . . 1318 1323 - BsmI GAATGC 1318 1316 . . 1327 1332 + ApoI RAATTY 1327 1331 . . 1328 1331 + TspEI AATT 1327 1331 . . 1331 1335 + Hpy188I TCNGA 1333 1332 . . 1334 1343 + BsaBI GATNNNNATC 1338 1338 . . 1339 1344 + XhoII RGATCY 1339 1343 . . 1339 1344 + BglII AGATCT 1339 1343 . . 1340 1343 + DpnI GATC 1341 1341 . . 1340 1343 + BstKTI GATC 1342 1340 . . 1340 1343 + MboI GATC 1339 1343 . . 1348 1351 + MnlI CCTC 1358 1357 . . 1356 1361 + AhaIII* TTTAAA 1358 1358 . . 1357 1360 + MseI TTAA 1357 1359 . . 1364 1370 + TspRI CASTGNN 1370 1361 . . 1376 1379 + TaqI TCGA 1376 1378 . . 1382 1385 + FatI CATG 1381 1385 . . 1382 1385 + NlaIII CATG 1385 1381 . . 1382 1385 + CviAII CATG 1382 1384 . . 1394 1398 + TspDTI ATGAA 1409 1407 . . 1396 1400 + MboII GAAGA 1408 1407 . . 1401 1405 + Tsp4CI* ACNGT 1403 1402 . . 1421 1425 + TspDTI ATGAA 1436 1434 . . 1427 1430 + TaqI TCGA 1427 1429 . . 1430 1433 + TspEI AATT 1429 1433 . . 1439 1443 + Hpy188I TCNGA 1441 1440 . . 1441 1446 + NlaIV GGNNCC 1443 1443 . . 1443 1446 + CviJI RGCY 1444 1444 . . 1445 1448 + MnlI CCTC 1455 1454 . . 1448 1462 + XcmI CCANNNNNNNNNTGG 1455 1454 . . 1461 1467 + DraII RGGNCCY 1462 1465 . . 1461 1466 + SduI GDGCHC 1465 1461 . . 1461 1466 + Bsp120I GGGCCC 1461 1465 . . 1461 1466 + NlaIV GGNNCC 1463 1463 . . 1461 1465 + BmgT120I GGNCC 1462 1463 . . 1461 1466 + HgiJII* GRGCYC 1465 1461 . . 1461 1466 + BseSI GKGCMC 1465 1461 . . 1461 1465 + AsuI* GGNCC 1461 1464 . . 1461 1466 + ApaI GGGCCC 1465 1461 . . 1462 1465 + CviJI RGCY 1463 1463 . . 1462 1465 + HaeIII GGCC 1463 1463 . . 1462 1466 + BmgT120I GGNCC 1463 1464 . . 1462 1466 + AsuI* GGNCC 1462 1465 . . 1494 1499 + ApoI RAATTY 1494 1498 . . 1495 1498 + TspEI AATT 1494 1498 . . 1501 1511 + Hin4I GAYNNNNNVTC 1492 1487 1524 1519 1507 1511 + BccI CCATC 1515 1516 . . 1501 1511 - Hin4I GAYNNNNNVTC 1524 1519 1492 1487 1513 1517 + Hpy188I TCNGA 1515 1514 . . 1510 1514 - MboII GAAGA 1502 1501 . . 1521 1524 + SetI ASST 1524 1520 . . 1522 1526 + BssKI CCNGG 1521 1526 . . 1522 1526 + BseBI CCWGG 1523 1524 . . 1522 1526 + ScrFI CCNGG 1523 1524 . . 1522 1526 + EcoRII CCWGG 1521 1526 . . 1530 1533 + CviJI RGCY 1531 1531 . . 1539 1543 + MaeIII GTNAC 1538 1543 . . 1543 1546 + AciI CCGC 1543 1545 . . 1570 1580 + MwoI GCNNNNNNNGC 1576 1573 . . 1574 1577 + SetI ASST 1577 1573 . . 1578 1581 + CviRI* TGCA 1579 1579 . . 1595 1598 + TspEI AATT 1594 1598 . . 1599 1604 + NlaIV GGNNCC 1601 1601 . . 1599 1604 + HgiCI* GGYRCC 1599 1603 . . 1610 1613 + MseI TTAA 1610 1612 . . 1625 1629 + DdeI CTNAG 1625 1628 . . 1631 1634 + TaqI TCGA 1631 1633 . . 1640 1646 + PfoI TCCNGGA 1640 1645 . . 1641 1645 + BssKI CCNGG 1640 1645 . . 1641 1645 + ScrFI CCNGG 1642 1643 . . 1641 1645 + CauII* CCSGG 1642 1643 . . 1642 1645 + HpaII CCGG 1642 1644 . . 1639 1643 - BslFI GGGAC 1624 1628 . . 1645 1649 + MboII GAAGA 1657 1656 . . 1648 1652 + TfiI GAWTC 1648 1651 . . 1648 1652 + HinfI GANTC 1648 1651 . . 1648 1657 + XmnI GAANNNNTTC 1652 1652 . . 1657 1662 + MfeI CAATTG 1657 1661 . . 1658 1661 + TspEI AATT 1657 1661 . . 1681 1686 + Hpy166II GTNNAC 1683 1683 . . 1681 1686 + HindII GTYRAC 1683 1683 . . 1695 1700 + Hpy166II GTNNAC 1697 1697 . . 1695 1700 + AccI GTMKAC 1696 1698 . . 1705 1710 + DsaI* CCRYGG 1705 1709 . . 1705 1710 + SecI* CCNNGG 1705 1709 . . 1707 1711 + Tsp4CI* ACNGT 1709 1708 . . 1715 1720 + Hpy166II GTNNAC 1717 1717 . . 1731 1736 + SmlI CTYRAG 1731 1735 . . 1731 1736 + Bce83I* CTTGAG 1752 1750 . . 1734 1737 - MnlI CCTC 1727 1726 . . 1738 1742 + TfiI GAWTC 1738 1741 . . 1738 1742 + HinfI GANTC 1738 1741 . . 1742 1746 + Hin4II* CCTTC 1752 1751 . . 1746 1752 + TspRI CASTGNN 1752 1743 . . 1753 1758 + ApoI RAATTY 1753 1757 . . 1754 1757 + TspEI AATT 1753 1757 . . 1760 1763 + MnlI CCTC 1770 1769 . . 1761 1765 + DdeI CTNAG 1761 1764 . . 1761 1765 + BspCNI CTCAG 1774 1772 . . 1761 1765 + BseMII CTCAG 1775 1773 . . 1762 1766 + Hpy188I TCNGA 1764 1763 . . 1771 1775 - MboII GAAGA 1763 1762 . . 1790 1793 + SetI ASST 1793 1789 . . 1789 1792 - MnlI CCTC 1782 1781 . . 1798 1801 + MseI TTAA 1798 1800 . . 1809 1812 + TspEI AATT 1808 1812 . . 1816 1819 + FatI CATG 1815 1819 . . 1816 1819 + NlaIII CATG 1819 1815 . . 1816 1819 + CviAII CATG 1816 1818 . . 1821 1826 + AhaIII* TTTAAA 1823 1823 . . 1822 1825 + MseI TTAA 1822 1824 . . 1826 1829 + SetI ASST 1829 1825 . . 1841 1846 + BsrDI GCAATG 1848 1846 . . 1850 1855 + NdeI CATATG 1851 1853 . . 1848 1852 - TspDTI ATGAA 1838 1836 . . 1858 1862 + MboII GAAGA 1870 1869 . . 1861 1865 + PleI GAGTC 1869 1870 . . 1861 1865 + MlyI GAGTC 1870 1870 . . 1861 1865 + HinfI GANTC 1861 1864 . . 1858 1863 - Ksp632I* CTCTTC 1853 1856 . . 1865 1868 + MnlI CCTC 1875 1874 . . 1866 1871 + Ksp632I* CTCTTC 1872 1875 . . 1867 1871 - MboII GAAGA 1859 1858 . . 1876 1881 + AclI AACGTT 1877 1879 . . 1877 1880 + TaiI ACGT 1880 1876 . . 1877 1880 + SetI ASST 1880 1876 . . 1877 1880 + MaeII ACGT 1877 1879 . . 1889 1892 + CviRI* TGCA 1890 1890 . . 1892 1897 + AclI AACGTT 1893 1895 . . 1893 1896 + TaiI ACGT 1896 1892 . . 1893 1896 + SetI ASST 1896 1892 . . 1893 1896 + MaeII ACGT 1893 1895 . . 1906 1910 + Hpy188I TCNGA 1908 1907 . . 1907 1912 + Eco57MI CTGRAG 1928 1926 . . 1907 1912 + Eco57I CTGAAG 1928 1926 . . 1911 1914 + SetI ASST 1914 1910 . . 1911 1914 + CviJI RGCY 1912 1912 . . 1911 1914 + AluI AGCT 1912 1912 . . 1931 1934 + SetI ASST 1934 1930 . . 1950 1954 + TfiI GAWTC 1950 1953 . . 1950 1954 + HinfI GANTC 1950 1953 . . 1953 1957 - MboII GAAGA 1945 1944 . . 1957 1960 + MaeI CTAG 1957 1959 . . 1998 2001 + CviRI* TGCA 1999 1999 . . 2025 2030 + BclI TGATCA 2025 2029 . . 2026 2029 + DpnI GATC 2027 2027 . . 2026 2029 + BstKTI GATC 2028 2026 . . 2026 2029 + MboI GATC 2025 2029 . . 2030 2035 + SspI AATATT 2032 2032 . . 2034 2039 + AhaIII* TTTAAA 2036 2036 . . 2035 2038 + MseI TTAA 2035 2037 . . 2053 2057 + MboII GAAGA 2065 2064 . . 2064 2073 + XmnI GAANNNNTTC 2068 2068 . . 2076 2087 + CspCI CAANNNNNGTGG 2064 2062 2099 2097 2078 2081 + TaiI ACGT 2081 2077 . . 2078 2081 + SetI ASST 2081 2077 . . 2078 2081 + MaeII ACGT 2078 2080 . . 2094 2098 + MboII GAAGA 2106 2105 . . 2097 2102 + EcoRI GAATTC 2097 2101 . . 2097 2102 + ApoI RAATTY 2097 2101 . . 2098 2101 + TspEI AATT 2097 2101 . . 2104 2108 + TspDTI ATGAA 2119 2117 . . 2108 2112 + TspDTI ATGAA 2123 2121 . . 2110 2114 + MboII GAAGA 2122 2121 . . 2120 2123 - AciI CCGC 2120 2122 . . 2128 2133 + Hpy166II GTNNAC 2130 2130 . . 2147 2150 + CviJI RGCY 2148 2148 . . 2149 2152 + MnlI CCTC 2159 2158 . . 2152 2157 + MfeI CAATTG 2152 2156 . . 2153 2156 + TspEI AATT 2152 2156 . . 2176 2179 + MseI TTAA 2176 2178 . . 2181 2190 + MslI CAYNNNNRTG 2185 2185 . . 2181 2186 + NdeI CATATG 2182 2184 . . 2202 2205 + SetI ASST 2205 2201 . . 2206 2210 + BsmAI GTCTC 2211 2215 . . 2239 2242 + CviJI RGCY 2240 2240 . . 2241 2244 + MnlI CCTC 2251 2250 . . 2245 2249 + Tsp4CI* ACNGT 2247 2246 . . 2243 2247 - HphI GGTGA 2235 2234 . . 2250 2255 + ApoI RAATTY 2250 2254 . . 2251 2254 + TspEI AATT 2250 2254 . . 2255 2260 - EcoP15I CAGCAG 2227 2229 . . 2260 2264 + HgaI GACGC 2269 2274 . . 2262 2265 + FnuDII* CGCG 2263 2263 . . 2263 2266 + Hin6I GCGC 2263 2265 . . 2263 2266 + GlaI GCGC 2264 2264 . . 2263 2266 + HhaI GCGC 2265 2263 . . 2268 2271 + TspEI AATT 2267 2271 . . 2280 2283 + SetI ASST 2283 2279 . . 2280 2283 + CviJI RGCY 2281 2281 . . 2280 2283 + AluI AGCT 2281 2281 . . 2289 2292 + TspEI AATT 2288 2292 . . 2299 2304 + HindIII AAGCTT 2299 2303 . . 2300 2303 + SetI ASST 2303 2299 . . 2300 2303 + CviJI RGCY 2301 2301 . . 2300 2303 + AluI AGCT 2301 2301 . . 2305 2309 + Hpy188I TCNGA 2307 2306 . . 2306 2310 + DdeI CTNAG 2306 2309 . . 2306 2310 - BspCNI CTCAG 2298 2296 . . 2306 2310 - BseMII CTCAG 2297 2295 . . 2317 2322 + Hpy178III* TCNNGA 2318 2320 . . 2308 2318 - Hin4I GAYNNNNNVTC 2331 2326 2299 2294 2320 2324 + BinI* GGATC 2328 2329 . . 2321 2324 + DpnI GATC 2322 2322 . . 2321 2324 + BstKTI GATC 2323 2321 . . 2321 2324 + MboI GATC 2320 2324 . . 2327 2332 + PvuII CAGCTG 2329 2329 . . 2327 2332 + NspBII* CMGCKG 2329 2329 . . 2328 2331 + SetI ASST 2331 2327 . . 2328 2331 + CviJI RGCY 2329 2329 . . 2328 2331 + AluI AGCT 2329 2329 . . 2339 2342 + TspEI AATT 2338 2342 . . 2341 2345 - TspDTI ATGAA 2331 2329 . . 2347 2351 + Hpy188I TCNGA 2349 2348 . . 2364 2367 + FatI CATG 2363 2367 . . 2364 2367 + NlaIII CATG 2367 2363 . . 2364 2367 + CviAII CATG 2364 2366 . . 2371 2381 + Hin4I GAYNNNNNVTC 2362 2357 2394 2389 2374 2379 + HaeII RGCGCY 2378 2374 . . 2375 2378 + Hin6I GCGC 2375 2377 . . 2375 2378 + GlaI GCGC 2376 2376 . . 2375 2378 + HhaI GCGC 2377 2375 . . 2378 2381 + MnlI CCTC 2388 2387 . . 2381 2384 + MnlI CCTC 2391 2390 . . 2379 2384 - BseRI GAGGAG 2370 2368 . . 2398 2408 + MwoI GCNNNNNNNGC 2404 2401 . . 2403 2406 + SetI ASST 2406 2402 . . 2403 2406 + CviJI RGCY 2404 2404 . . 2403 2406 + AluI AGCT 2404 2404 . . 2404 2408 + BlsI GCNGC 2406 2405 . . 2404 2408 + BisI GCNGC 2405 2406 . . 2404 2408 + TseI GCWGC 2404 2407 . . 2406 2409 + CviRI* TGCA 2407 2407 . . 2404 2408 - BbvI GCAGC 2391 2395 . . 2413 2416 + MseI TTAA 2413 2415 . . 2421 2424 + TspEI AATT 2420 2424 . . 2423 2426 + MseI TTAA 2423 2425 . . 2438 2441 + TspEI AATT 2437 2441 . . 2445 2448 + SetI ASST 2448 2444 . . 2452 2455 + DpnI GATC 2453 2453 . . 2452 2455 + BstKTI GATC 2454 2452 . . 2452 2455 + MboI GATC 2451 2455 . . 2457 2462 + Hpy178III* TCNNGA 2458 2460 . . 2464 2468 - TspDTI ATGAA 2454 2452 . . 2476 2480 + Tsp4CI* ACNGT 2478 2477 . . 2478 2482 + Hpy99I CGWCG 2482 2477 . . 2483 2487 + DdeI CTNAG 2483 2486 . . 2486 2489 + SetI ASST 2489 2485 . . 2483 2487 - BspCNI CTCAG 2475 2473 . . 2483 2487 - BseMII CTCAG 2474 2472 . . 2485 2488 - MnlI CCTC 2478 2477 . . 2482 2488 - Bpu10I CCTNAGC 2483 2486 . . 2482 2488 - BbvCI CCTCAGC 2483 2486 . . 2497 2502 + TatI WGTACW 2497 2501 . . 2498 2501 + Csp6I GTAC 2498 2500 . . 2498 2501 + RsaI GTAC 2499 2499 . . 2505 2509 + FokI GGATG 2518 2522 . . 2505 2509 + BseGI GGATG 2511 2509 . . 2513 2517 + TspDTI ATGAA 2528 2526 . . 2521 2526 + XbaI TCTAGA 2521 2525 . . 2521 2526 + Hpy178III* TCNNGA 2522 2524 . . 2522 2525 + MaeI CTAG 2522 2524 . . 2527 2531 - BsmAI GTCTC 2521 2525 . . 2531 2534 + AciI CCGC 2531 2533 . . 2527 2532 - Eco31I GGTCTC 2521 2525 . . 2545 2548 + MseI TTAA 2545 2547 . . 2547 2550 + TspEI AATT 2546 2550 . . 2558 2562 + MboII GAAGA 2570 2569 . . 2566 2570 + TspDTI ATGAA 2581 2579 . . 2573 2576 + CviJI RGCY 2574 2574 . . 2591 2594 + TspEI AATT 2590 2594 . . 2604 2610 + SexAI ACCWGGT 2604 2609 . . 2605 2609 + BssKI CCNGG 2604 2609 . . 2605 2609 + BseBI CCWGG 2606 2607 . . 2605 2609 + ScrFI CCNGG 2606 2607 . . 2605 2609 + EcoRII CCWGG 2604 2609 . . 2607 2610 + SetI ASST 2610 2606 . . 2614 2617 + DpnI GATC 2615 2615 . . 2614 2617 + BstKTI GATC 2616 2614 . . 2614 2617 + MboI GATC 2613 2617 . . 2614 2618 - BinI* GGATC 2608 2609 . . 2625 2630 + Hpy178III* TCNNGA 2626 2628 . . 2629 2639 + Hin4I GAYNNNNNVTC 2620 2615 2652 2647 2624 2629 - Eco57MI CTGRAG 2609 2607 . . 2629 2632 + DpnI GATC 2630 2630 . . 2629 2632 + BstKTI GATC 2631 2629 . . 2624 2629 - GsuI CTGGAG 2609 2607 . . 2629 2632 + MboI GATC 2628 2632 . . 2636 2640 + Hpy99I CGWCG 2640 2635 . . 2629 2639 - Hin4I GAYNNNNNVTC 2652 2647 2620 2615 2647 2651 + Hpy188I TCNGA 2649 2648 . . 2657 2660 + MseI TTAA 2657 2659 . . 2661 2664 + DpnI GATC 2662 2662 . . 2661 2664 + BstKTI GATC 2663 2661 . . 2661 2664 + MboI GATC 2660 2664 . . 2671 2676 + Cfr10I RCCGGY 2671 2675 . . 2672 2675 + HpaII CCGG 2672 2674 . . 2683 2687 + HphI GGTGA 2695 2694 . . 2686 2690 + TfiI GAWTC 2686 2689 . . 2686 2690 + HinfI GANTC 2686 2689 . . 2695 2700 + NdeI CATATG 2696 2698 . . 2699 2704 - TsoI TARCCA 2689 2687 . . 2712 2715 + TspEI AATT 2711 2715 . . 2740 2745 + EcoRI GAATTC 2740 2744 . . 2740 2745 + ApoI RAATTY 2740 2744 . . 2741 2744 + TspEI AATT 2740 2744 . . 2750 2754 + TspDTI ATGAA 2765 2763 . . 2756 2759 + TaqI TCGA 2756 2758 . . 2758 2762 + TfiI GAWTC 2758 2761 . . 2758 2762 + HinfI GANTC 2758 2761 . . 2761 2766 + BetI* WCCGGW 2761 2765 . . 2762 2765 + HpaII CCGG 2762 2764 . . 2765 2770 + Hpy166II GTNNAC 2767 2767 . . 2765 2770 + BssNAI GTATAC 2767 2767 . . 2765 2770 + AccI GTMKAC 2766 2768 . . 2795 2798 + TspEI AATT 2794 2798 . . 2798 2803 + TatI WGTACW 2798 2802 . . 2799 2802 + Csp6I GTAC 2799 2801 . . 2799 2802 + RsaI GTAC 2800 2800 . . 2803 2806 + MseI TTAA 2803 2805 . . 2816 2819 + MaeI CTAG 2816 2818 . . 2823 2826 + TspEI AATT 2822 2826 . . 2833 2838 + HaeII RGCGCY 2837 2833 . . 2834 2837 + Hin6I GCGC 2834 2836 . . 2834 2837 + GlaI GCGC 2835 2835 . . 2834 2837 + HhaI GCGC 2836 2834 . . 2844 2849 + Hpy166II GTNNAC 2846 2846 . . 2849 2854 + StyI CCWWGG 2849 2853 . . 2849 2854 + SecI* CCNNGG 2849 2853 . . 2852 2855 + SetI ASST 2855 2851 . . 2867 2870 + CviJI RGCY 2868 2868 . . 2871 2876 + Hpy178III* TCNNGA 2872 2874 . . 2875 2879 + MboII GAAGA 2887 2886 . . 2888 2891 + CviJI RGCY 2889 2889 . . 2888 2891 + HaeIII GGCC 2889 2889 . . 2886 2889 - MnlI CCTC 2879 2878 . . 2889 2899 + MwoI GCNNNNNNNGC 2895 2892 . . 2894 2897 + SetI ASST 2897 2893 . . 2895 2900 + NlaIV GGNNCC 2897 2897 . . 2895 2900 + HgiCI* GGYRCC 2895 2899 . . 2904 2908 + MaeIII GTNAC 2903 2908 . . 2920 2923 + MseI TTAA 2920 2922 . . 2927 2932 + Eco31I GGTCTC 2933 2937 . . 2924 2928 - BccI CCATC 2918 2919 . . 2928 2932 + BsmAI GTCTC 2933 2937 . . 2931 2936 + Hpy178III* TCNNGA 2932 2934 . . 2940 2946 + TspRI CASTGNN 2946 2937 . . 2941 2945 + BsrI ACTGG 2946 2944 . . 2955 2958 + CviJI RGCY 2956 2956 . . 2982 2987 + VspI ATTAAT 2983 2985 . . 2983 2986 + MseI TTAA 2983 2985 . . 3015 3018 + CviJI RGCY 3016 3016 . . 3026 3031 + Eco57MI CTGRAG 3047 3045 . . 3026 3031 + Eco57I CTGAAG 3047 3045 . . 3028 3032 + MboII GAAGA 3040 3039 . . 3031 3035 + MboII GAAGA 3043 3042 . . 3046 3049 + MseI TTAA 3046 3048 . . 3065 3070 + MstI* TGCGCA 3067 3067 . . 3066 3069 + Hin6I GCGC 3066 3068 . . 3066 3069 + GlaI GCGC 3067 3067 . . 3066 3069 + HhaI GCGC 3068 3066 . . 3063 3067 - SfaNI GCATC 3053 3057 . . 3089 3093 + TspDTI ATGAA 3104 3102 . . 3091 3095 + MboII GAAGA 3103 3102 . . 3097 3101 + MaeIII GTNAC 3096 3101 . . 3103 3107 + BsrI ACTGG 3108 3106 . . 3104 3109 + Eco57MI CTGRAG 3125 3123 . . 3104 3109 + GsuI CTGGAG 3125 3123 . . 3120 3123 + SetI ASST 3123 3119 . . 3120 3123 + CviJI RGCY 3121 3121 . . 3120 3123 + AluI AGCT 3121 3121 . . 3121 3125 + BlsI GCNGC 3123 3122 . . 3121 3125 + BisI GCNGC 3122 3123 . . 3121 3125 + TseI GCWGC 3121 3124 . . 3121 3125 - BbvI GCAGC 3108 3112 . . 3136 3141 + Hpy178III* TCNNGA 3137 3139 . . 3150 3153 + CviRI* TGCA 3151 3151 . . 3155 3158 + TaqI TCGA 3155 3157 . . 3151 3156 - BsmI GAATGC 3151 3149 . . 3158 3162 + TspDTI ATGAA 3173 3171 . . 3162 3165 + SetI ASST 3165 3161 . . 3162 3165 + CviJI RGCY 3163 3163 . . 3162 3165 + AluI AGCT 3163 3163 . . 3175 3179 + Hpy188I TCNGA 3177 3176 . . 3179 3183 + TspDTI ATGAA 3194 3192 . . 3181 3185 + MboII GAAGA 3193 3192 . . 3190 3200 + Hin4I GAYNNNNNVTC 3181 3176 3213 3208 3196 3200 + BinI* GGATC 3204 3205 . . 3197 3200 + DpnI GATC 3198 3198 . . 3197 3200 + BstKTI GATC 3199 3197 . . 3197 3200 + MboI GATC 3196 3200 . . 3199 3203 + Hpy188I TCNGA 3201 3200 . . 3190 3200 - Hin4I GAYNNNNNVTC 3213 3208 3181 3176 3202 3205 + DpnI GATC 3203 3203 . . 3202 3205 + BstKTI GATC 3204 3202 . . 3202 3205 + MboI GATC 3201 3205 . . 3204 3209 + Hpy178III* TCNNGA 3205 3207 . . 3208 3211 - MnlI CCTC 3201 3200 . . 3232 3236 + TspDTI ATGAA 3247 3245 . . 3234 3238 + MboII GAAGA 3246 3245 . . 3251 3254 + TspEI AATT 3250 3254 . . 3262 3266 + TfiI GAWTC 3262 3265 . . 3262 3266 + HinfI GANTC 3262 3265 . . 3265 3270 + BetI* WCCGGW 3265 3269 . . 3266 3269 + HpaII CCGG 3266 3268 . . 3268 3272 + HphI GGTGA 3280 3279 . . 3269 3273 + Tsp45I GTSAC 3268 3273 . . 3269 3273 + MaeIII GTNAC 3268 3273 . . 3280 3283 + MaeI CTAG 3280 3282 . . 3277 3288 - CspCI CAANNNNNGTGG 3301 3299 3266 3264 3292 3296 + TfiI GAWTC 3292 3295 . . 3292 3296 + HinfI GANTC 3292 3295 . . 3304 3307 + MaeI CTAG 3304 3306 . . 3306 3309 + SetI ASST 3309 3305 . . 3306 3309 + CviJI RGCY 3307 3307 . . 3306 3309 + AluI AGCT 3307 3307 . . 3334 3339 + ApoI RAATTY 3334 3338 . . 3335 3338 + TspEI AATT 3334 3338 . . 3345 3349 + TfiI GAWTC 3345 3348 . . 3345 3349 + HinfI GANTC 3345 3348 . . 3361 3364 + SetI ASST 3364 3360 . . 3365 3368 + MseI TTAA 3365 3367 . . 3373 3377 - SfaNI GCATC 3363 3367 . . 3380 3383 + MseI TTAA 3380 3382 . . 3388 3392 + HinfI GANTC 3388 3391 . . 3390 3394 + DdeI CTNAG 3390 3393 . . 3390 3394 + BspCNI CTCAG 3403 3401 . . 3390 3394 + BseMII CTCAG 3404 3402 . . 3391 3395 + Hpy188I TCNGA 3393 3392 . . 3388 3392 - PleI GAGTC 3382 3383 . . 3388 3392 - MlyI GAGTC 3382 3382 . . 3394 3398 - Hin4II* CCTTC 3388 3387 . . 3420 3423 + SetI ASST 3423 3419 . . 3428 3431 + MnlI CCTC 3438 3437 . . 3439 3444 + Hpy166II GTNNAC 3441 3441 . . 3439 3444 + AccI GTMKAC 3440 3442 . . 3445 3448 + DpnI GATC 3446 3446 . . 3445 3448 + BstKTI GATC 3447 3445 . . 3445 3448 + MboI GATC 3444 3448 . . 3445 3449 - BinI* GGATC 3439 3440 . . 3460 3464 + Tsp45I GTSAC 3459 3464 . . 3460 3464 + MaeIII GTNAC 3459 3464 . . 3470 3474 + TfiI GAWTC 3470 3473 . . 3470 3474 + HinfI GANTC 3470 3473 . . 3467 3470 - AciI CCGC 3467 3469 . . 3483 3488 + XbaI TCTAGA 3483 3487 . . 3483 3488 + Hpy178III* TCNNGA 3484 3486 . . 3484 3487 + MaeI CTAG 3484 3486 . . 3489 3492 + CviJI RGCY 3490 3490 . . 3487 3490 - MnlI CCTC 3480 3479 . . 3502 3506 + BmgT120I GGNCC 3503 3504 . . 3502 3506 + AvaII GGWCC 3502 3505 . . 3502 3506 + AsuI* GGNCC 3502 3505 . . 3505 3515 + EcoNI CCTNNNNNAGG 3509 3510 . . 3505 3515 + BsiYI* CCNNNNNNNGG 3511 3508 . . 3513 3516 + SetI ASST 3516 3512 . . 3512 3515 - MnlI CCTC 3505 3504 . . 3527 3530 + SetI ASST 3530 3526 . . 3527 3530 + CviJI RGCY 3528 3528 . . 3527 3530 + AluI AGCT 3528 3528 . . 3532 3537 + ApoI RAATTY 3532 3536 . . 3533 3536 + TspEI AATT 3532 3536 . . 3536 3541 + Hpy178III* TCNNGA 3537 3539 . . 3553 3557 + HinfI GANTC 3553 3556 . . 3556 3560 + Hpy188I TCNGA 3558 3557 . . 3553 3557 - PleI GAGTC 3547 3548 . . 3553 3557 - MlyI GAGTC 3547 3547 . . 3560 3564 + TspDTI ATGAA 3575 3573 . . 3562 3567 + ApoI RAATTY 3562 3566 . . 3563 3566 + TspEI AATT 3562 3566 . . 3569 3574 + BtgZI GCGATG 3584 3588 . . 3583 3587 + HphI GGTGA 3595 3594 . . 3580 3584 - BccI CCATC 3574 3575 . . 3599 3602 + CviJI RGCY 3600 3600 . . 3608 3611 - MnlI CCTC 3601 3600 . . 3611 3615 + MboII GAAGA 3623 3622 . . 3611 3616 - Ksp632I* CTCTTC 3606 3609 . . 3624 3627 + SetI ASST 3627 3623 . . 3626 3630 + MaeIII GTNAC 3625 3630 . . 3654 3657 + SetI ASST 3657 3653 . . 3655 3659 + BmgT120I GGNCC 3656 3657 . . 3655 3659 + AvaII GGWCC 3655 3658 . . 3655 3659 + AsuI* GGNCC 3655 3658 . . 3670 3675 + XbaI TCTAGA 3670 3674 . . 3670 3675 + Hpy178III* TCNNGA 3671 3673 . . 3671 3674 + MaeI CTAG 3671 3673 . . 3680 3683 + TspEI AATT 3679 3683 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 212 247 277 513 674 694 # 1070 # # AciI: # [CCGC] # 45 220 411 553 577 936 # 945 # # AclI: # [AACGTT] # 16 1794 1877 # # AflII: # [CTTAAG] # 1300 2387 # # AhaIII*: # [TTTAAA] # 213 465 647 711 1651 # # AluI: # [AGCT] # 12 20 27 28 33 42 # 75 97 144 159 221 246 # 275 369 433 717 789 # # ApaI: # [GGGCCC] # 1465 2222 # # ApaLI: # [GTGCAC] # 1083 2604 # # ApoI: # [RAATTY] # 30 32 53 125 153 167 # 198 237 259 344 398 490 # 594 607 # # AsuI*: # [GGNCC] # 1 32 153 1461 2040 # # AvaII: # [GGWCC] # 32 153 3502 # # BalI: # [TGGCCA] # 768 2919 # # BbvCI: # [CCTCAGC] # 1204 2483 # # BbvI: # [GCAGC] # 217 375 579 717 735 1064 # # BbvII*: # [GAAGAC] # 707 2980 # # BccI: # [CCATC] # 113 225 552 656 738 1403 # # Bce83I*: # [CTTGAG] # 407 1345 1935 # # BceAI: # [ACGGC] # 100 3587 # # BciVI: # [GTATCC] # 930 2757 # # BclI: # [TGATCA] # 1662 2025 # # BetI*: # [WCCGGW] # 422 504 2761 # # BglII: # [AGATCT] # 273 1066 2348 # # BinI*: # [GGATC] # 11 198 235 248 280 596 # 2119 # # BisI: # [GCNGC] # 192 210 389 525 565 717 # 1089 # # BlsI: # [GCNGC] # 192 210 390 525 564 717 # 1089 # # BmgT120I: # [GGNCC] # 1 31 153 1462 2040 # # Bpu10I: # [CCTNAGC] # 1204 2483 # # BsaAI: # [YACGTR] # 1054 2633 # # BsaBI: # [GATNNNNATC] # 1338 2349 # # BseBI: # [CCWGG] # 74 438 459 552 1081 1083 # # BseGI: # [GGATG] # 1176 1203 1308 # # BseMII: # [CTCAG] # 177 283 309 522 630 836 # 930 # # BseRI: # [GAGGAG] # 1317 2370 # # BseSI: # [GKGCMC] # 378 1087 2222 # # BsiYI*: # [CCNNNNNNNGG] # 176 475 514 2522 # # BslFI: # [GGGAC] # 1624 2063 # # BsmAI: # [GTCTC] # 310 412 754 756 1455 # # BsmI: # [GAATGC] # 536 1318 1833 # # Bsp120I: # [GGGCCC] # 1461 2226 # # BspCNI: # [CTCAG] # 177 284 311 524 628 835 # 928 # # BspHI: # [TCATGA] # 225 3462 # # BspMI: # [ACCTGC] # 124 504 3059 # # BsrDI: # [GCAATG] # 1839 1848 # # BsrI: # [ACTGG] # 162 579 762 2184 # # BssKI: # [CCNGG] # 74 119 436 459 552 964 # 1083 # # BssNAI: # [GTATAC] # 490 694 920 1583 # # BstAPI: # [GCANNNNNTGC] # 33 550 3104 # # BstKTI: # [GATC] # 5 6 15 32 37 72 # 131 143 162 198 198 240 # 243 295 536 686 688 # # BtgZI: # [GCGATG] # 103 3584 # # BtsI: # [GCAGTG] # 353 3334 # # Cac8I: # [GCNNGC] # 47 3640 # # CauII*: # [CCSGG] # 1642 2045 # # Cfr10I: # [RCCGGY] # 1016 2671 # # CfrI: # [YGGCCR] # 766 2921 # # Csp6I: # [GTAC] # 122 195 301 799 888 1382 # # CspCI: # [CAANNNNNGTGG] # 35 35 386 1167 2064 # # CviAII: # [CATG] # 42 121 128 147 203 226 # 434 515 548 1323 # # CviJI: # [RGCY] # 4 12 18 19 20 21 # 21 27 28 33 35 38 # 41 42 48 60 67 68 # 72 75 87 89 92 93 # 105 108 117 144 170 183 # 186 236 294 321 332 381 # # CviRI*: # [TGCA] # 9 33 53 61 72 105 # 109 133 136 225 258 311 # 408 494 536 744 # # DdeI: # [CTNAG] # 92 103 136 177 229 296 # 297 434 471 545 907 # # DpnI: # [GATC] # 5 5 15 32 37 72 # 131 143 162 198 198 241 # 243 295 536 686 688 # # DraII: # [RGGNCCY] # 1462 2225 # # DraIII: # [CACNNNGTG] # 1192 2495 # # DsaI*: # [CCRYGG] # 1705 1982 # # Eco31I: # [GGTCTC] # 412 754 2521 # # Eco47III: # [AGCGCT] # 513 536 2638 # # Eco57I: # [CTGAAG] # 640 728 1119 1200 # # Eco57MI: # [CTGRAG] # 55 78 363 438 562 673 # 681 837 # # EcoNI: # [CCTNNNNNAGG] # 178 3509 # # EcoP15I: # [CAGCAG] # 1460 2227 # # EcoRI: # [GAATTC] # 643 947 2097 # # EcoRII: # [CCWGG] # 74 436 459 552 1083 1083 # # Esp3I: # [CGTCTC] # 756 2931 # # FatI: # [CATG] # 42 121 128 147 203 225 # 434 515 548 1324 # # FnuDII*: # [CGCG] # 1424 2263 # # FokI: # [GGATG] # 1169 1210 1308 # # GlaI: # [GCGC] # 112 188 232 325 459 536 # 620 1215 # # GsuI: # [CTGGAG] # 55 516 562 1036 1518 # # HaeII: # [RGCGCY] # 459 513 538 850 1327 # # HaeIII: # [GGCC] # 695 768 798 1426 # # HgaI: # [GACGC] # 1418 2269 # # HgiAI*: # [GWGCWC] # 1087 2600 # # HgiCI*: # [GGYRCC] # 792 1296 1599 # # HgiJII*: # [GRGCYC] # 1465 2222 # # HhaI: # [GCGC] # 112 188 232 325 459 537 # 619 1215 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 0 0 # 0 0 31 32 32 32 # 32 32 32 32 211 226 # 446 474 529 771 775 # # Hin4II*: # [CCTTC] # 299 1636 1752 # # Hin6I: # [GCGC] # 112 188 232 325 459 535 # 621 1215 # # HindII: # [GTYRAC] # 1683 2004 # # HindIII: # [AAGCTT] # 1388 2299 # # HinfI: # [GANTC] # 30 43 53 72 82 83 # 84 89 90 120 123 134 # 227 322 403 492 504 736 # # HpaII: # [CCGG] # 90 421 504 1030 1642 # # HphI: # [GGTGA] # 92 315 460 585 804 1431 # # Hpy166II: # [GTNNAC] # 14 20 31 66 68 69 # 79 144 246 277 413 499 # 529 595 637 # # Hpy178III*: # [TCNNGA] # 7 16 45 53 60 64 # 68 104 134 140 153 181 # 205 206 219 233 245 246 # 279 1029 # # Hpy188I: # [TCNGA] # 24 42 46 74 108 129 # 144 165 178 192 249 278 # 300 330 399 501 528 # # Hpy99I: # [CGWCG] # 158 1047 2482 # # Ksp632I*: # [CTCTTC] # 19 81 416 1437 1734 # # MaeI: # [CTAG] # 16 24 175 180 187 267 # 271 294 384 464 565 860 # # MaeII: # [ACGT] # 16 164 185 461 592 660 # 1609 # # MaeIII: # [GTNAC] # 62 160 166 172 188 191 # 191 193 271 728 1365 # # MboI: # [GATC] # 3 5 15 32 37 72 # 131 143 162 198 198 243 # 243 295 536 686 688 # # MboII: # [GAAGA] # 3 11 16 41 52 53 # 60 64 75 90 94 96 # 106 120 126 144 153 155 # 259 309 317 377 448 518 # # MfeI: # [CAATTG] # 244 432 495 981 1535 # # MlyI: # [GAGTC] # 120 140 165 227 705 818 # 1512 # # MnlI: # [CCTC] # 3 12 25 30 42 43 # 83 84 86 87 92 93 # 96 97 109 122 137 150 # 168 172 219 221 237 272 # 284 322 401 # # MseI: # [TTAA] # 10 15 24 27 28 56 # 61 63 63 84 109 112 # 117 122 138 141 146 156 # 188 198 213 237 245 253 # 255 307 319 # # MslI: # [CAYNNNNRTG] # 852 1333 1502 # # MstI*: # [TGCGCA] # 620 3067 # # MwoI: # [GCNNNNNNNGC] # 9 33 63 114 139 225 # 491 792 828 993 # # NdeI: # [CATATG] # 331 514 991 1851 # # NlaIII: # [CATG] # 42 121 128 147 203 229 # 434 515 548 1320 # # NlaIV: # [GGNNCC] # 20 138 790 1296 1443 # # NspBII*: # [CMGCKG] # 1358 2329 # # NspI: # [RCATGY] # 275 595 2817 # # PflMI: # [CCANNNNNTGG] # 989 2698 # # PfoI: # [TCCNGGA] # 652 988 2047 # # PleI: # [GAGTC] # 120 140 165 227 705 817 # 1513 # # PstI: # [CTGCAG] # 228 360 3099 # # PvuII: # [CAGCTG] # 1358 2329 # # RsaI: # [GTAC] # 122 195 301 800 887 1382 # # SauI*: # [CCTNAGG] # 296 3391 # # ScrFI: # [CCNGG] # 74 119 438 459 552 964 # 1081 # # SduI: # [GDGCHC] # 378 1087 2222 # # SecI*: # [CCNNGG] # 838 1144 1705 # # SetI: # [ASST] # 6 12 14 16 16 18 # 20 20 27 28 30 30 # 35 36 41 42 42 42 # 42 51 53 55 59 74 # 75 78 87 88 91 93 # 94 95 97 103 121 124 # 130 144 147 158 162 216 # 226 245 304 # # SexAI: # [ACCWGGT] # 1083 2604 # # SfaNI: # [GCATC] # 114 310 322 324 417 2200 # # SfeI*: # [CTRYAG] # 228 356 3103 # # SmlI: # [CTYRAG] # 422 431 878 1956 # # SnaBI: # [TACGTA] # 1054 2633 # # SspI: # [AATATT] # 22 107 1655 1903 # # StyI: # [CCWWGG] # 838 2849 # # TaiI: # [ACGT] # 16 164 185 464 592 660 # 1606 # # TaqI: # [TCGA] # 51 120 204 288 310 313 # 345 399 532 1125 # # TatI: # [WGTACW] # 301 798 889 1699 # # TauI: # [GCSGC] # 601 3086 # # TfiI: # [GAWTC] # 30 53 72 84 90 125 # 212 217 322 504 736 1242 # # TseI: # [GCWGC] # 192 388 566 717 735 1089 # # TsoI: # [TARCCA] # 37 959 998 1693 # # Tsp45I: # [GTSAC] # 160 188 191 191 228 271 # 2458 # # Tsp4CI*: # [ACNGT] # 231 306 538 639 764 1209 # # TspDTI: # [ATGAA] # 4 21 27 32 53 53 # 69 74 112 123 171 184 # 208 242 264 281 328 339 # 402 700 # # TspEI: # [AATT] # 8 16 17 17 21 23 # 28 29 30 32 42 44 # 50 53 54 55 55 57 # 63 65 73 82 84 94 # 95 96 98 100 102 109 # 117 121 180 198 280 289 # 382 428 # # TspRI: # [CASTGNN] # 277 324 360 382 409 741 # 1194 # # VspI: # [ATTAAT] # 704 2983 # # XbaI: # [TCTAGA] # 17 187 270 962 2251 # # XcmI: # [CCANNNNNNNNNTGG] # 1455 2232 # # XhoII: # [RGATCY] # 273 1066 2348 # # XmnI: # [GAANNNNTTC] # 410 416 445 797 1619 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 3687 # Reported_sequences: 1 # Reported_hitcount: 810 #---------------------------------------