Restriction Fragments for CHL1/YPL008W



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The currently selected gene/sequence is :

CHL1/YPL008W on chromosome XVI from coordinates 539385 to 541970.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.27497.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 14 19 + SspI AATATT 16 16 . . 19 23 + Hpy188I TCNGA 21 20 . . 24 34 + Hin4I GAYNNNNNVTC 15 10 47 42 22 26 - BsmAI GTCTC 16 20 . . 24 34 - Hin4I GAYNNNNNVTC 47 42 15 10 34 38 - FokI GGATG 20 24 . . 34 38 - BseGI GGATG 33 31 . . 39 44 + PsiI TTATAA 41 41 . . 44 47 + CviJI RGCY 45 45 . . 59 62 + SetI ASST 62 58 . . 61 64 + Csp6I GTAC 61 63 . . 61 64 + RsaI GTAC 62 62 . . 63 67 + Tsp4CI* ACNGT 65 64 . . 67 70 + MseI TTAA 67 69 . . 76 80 + Tsp4CI* ACNGT 78 77 . . 79 84 + Hpy166II GTNNAC 81 81 . . 79 84 + BssNAI GTATAC 81 81 . . 79 84 + AccI GTMKAC 80 82 . . 94 98 + Hpy188I TCNGA 96 95 . . 97 101 - Hin4II* CCTTC 91 90 . . 102 106 + MboII GAAGA 114 113 . . 111 114 + SetI ASST 114 110 . . 111 114 + CviJI RGCY 112 112 . . 111 114 + AluI AGCT 112 112 . . 116 122 + PfoI TCCNGGA 116 121 . . 117 121 + BssKI CCNGG 116 121 . . 117 121 + BseBI CCWGG 118 119 . . 117 121 + ScrFI CCNGG 118 119 . . 117 121 + EcoRII CCWGG 116 121 . . 124 127 + CviJI RGCY 125 125 . . 129 135 + TspRI CASTGNN 135 126 . . 130 134 + BsrI ACTGG 135 133 . . 130 135 + BfiI ACTGGG 140 139 . . 133 137 + BslFI GGGAC 147 151 . . 144 148 + HgaI GACGC 153 158 . . 144 152 + Tth111I GACNNNGTC 147 148 . . 154 157 + MseI TTAA 154 156 . . 184 188 + TspDTI ATGAA 199 197 . . 204 213 + MslI CAYNNNNRTG 208 208 . . 206 210 + FauI CCCGC 214 216 . . 203 207 - HphI GGTGA 195 194 . . 207 210 + AciI CCGC 207 209 . . 210 213 + FatI CATG 209 213 . . 210 213 + NlaIII CATG 213 209 . . 210 213 + CviAII CATG 210 212 . . 233 237 + TspDTI ATGAA 248 246 . . 235 239 + MboII GAAGA 247 246 . . 251 254 + SetI ASST 254 250 . . 252 258 + Bpu10I CCTNAGC 253 256 . . 253 257 + DdeI CTNAG 253 256 . . 257 262 + BtgZI GCGATG 272 276 . . 266 269 + CviJI RGCY 267 267 . . 272 276 + BsrI ACTGG 277 275 . . 272 277 + BfiI ACTGGG 282 281 . . 291 294 + TaqI TCGA 291 293 . . 314 317 + SetI ASST 317 313 . . 345 350 + AhaIII* TTTAAA 347 347 . . 346 349 + MseI TTAA 346 348 . . 354 357 + TspEI AATT 353 357 . . 356 359 + MseI TTAA 356 358 . . 365 369 - BsrI ACTGG 365 363 . . 409 412 + FatI CATG 408 412 . . 409 412 + NlaIII CATG 412 408 . . 409 412 + CviAII CATG 409 411 . . 413 417 + MboII GAAGA 425 424 . . 416 425 + BsaBI GATNNNNATC 420 420 . . 430 433 + DpnI GATC 431 431 . . 430 433 + BstKTI GATC 432 430 . . 430 433 + MboI GATC 429 433 . . 430 434 - BinI* GGATC 424 425 . . 436 439 + MseI TTAA 436 438 . . 454 459 + HaeII RGCGCY 458 454 . . 455 458 + Hin6I GCGC 455 457 . . 455 458 + GlaI GCGC 456 456 . . 455 458 + HhaI GCGC 457 455 . . 458 461 + MaeI CTAG 458 460 . . 461 466 + NlaIV GGNNCC 463 463 . . 461 466 + HgiCI* GGYRCC 461 465 . . 463 472 + MslI CAYNNNNRTG 467 467 . . 464 467 + SetI ASST 467 463 . . 481 485 + MboII GAAGA 493 492 . . 499 502 + AciI CCGC 499 501 . . 500 503 + FnuDII* CGCG 501 501 . . 501 504 + Hin6I GCGC 501 503 . . 501 504 + GlaI GCGC 502 502 . . 501 504 + HhaI GCGC 503 501 . . 506 511 + SplI* CGTACG 506 510 . . 507 510 + Csp6I GTAC 507 509 . . 507 510 + RsaI GTAC 508 508 . . 511 515 + TfiI GAWTC 511 514 . . 511 515 + HinfI GANTC 511 514 . . 514 518 + Hpy188I TCNGA 516 515 . . 515 523 + AlwNI CAGNNNCTG 520 517 . . 517 521 + TfiI GAWTC 517 520 . . 517 521 + HinfI GANTC 517 520 . . 520 524 + Hpy188I TCNGA 522 521 . . 535 538 + SetI ASST 538 534 . . 536 539 + MnlI CCTC 546 545 . . 551 556 + BseRI GAGGAG 566 564 . . 551 554 - MnlI CCTC 544 543 . . 554 557 - MnlI CCTC 547 546 . . 557 561 + MboII GAAGA 569 568 . . 565 569 + Hpy188I TCNGA 567 566 . . 581 584 + TspEI AATT 580 584 . . 587 592 + Hpy166II GTNNAC 589 589 . . 594 599 + ApoI RAATTY 594 598 . . 595 598 + TspEI AATT 594 598 . . 624 627 + TspEI AATT 623 627 . . 628 632 - BccI CCATC 622 623 . . 635 638 + SetI ASST 638 634 . . 633 637 - Hin4II* CCTTC 627 626 . . 640 643 + TaqI TCGA 640 642 . . 640 645 + Hpy178III* TCNNGA 641 643 . . 645 650 + XhoII RGATCY 645 649 . . 646 649 + DpnI GATC 647 647 . . 646 649 + BstKTI GATC 648 646 . . 646 649 + MboI GATC 645 649 . . 649 659 + BsiYI* CCNNNNNNNGG 655 652 . . 649 659 + PflMI CCANNNNNTGG 655 652 . . 646 650 - BinI* GGATC 640 641 . . 659 666 + SgfI GCGATCGC 663 661 . . 660 665 + McrI* CGRYCG 663 661 . . 660 665 + PvuI CGATCG 663 661 . . 661 664 + DpnI GATC 662 662 . . 661 664 + BstKTI GATC 663 661 . . 661 664 + MboI GATC 660 664 . . 671 674 + TaiI ACGT 674 670 . . 671 674 + SetI ASST 674 670 . . 671 674 + MaeII ACGT 671 673 . . 673 677 + MaeIII GTNAC 672 677 . . 689 695 + TspRI CASTGNN 695 686 . . 688 692 - BsrI ACTGG 688 686 . . 704 709 + EcoT22I ATGCAT 708 704 . . 705 708 + CviRI* TGCA 706 706 . . 706 710 + SfaNI GCATC 715 719 . . 709 714 + Hpy178III* TCNNGA 710 712 . . 707 711 - FokI GGATG 693 697 . . 707 711 - BseGI GGATG 706 704 . . 713 722 + XmnI GAANNNNTTC 717 717 . . 725 728 + TspEI AATT 724 728 . . 729 732 + SetI ASST 732 728 . . 734 737 + TspEI AATT 733 737 . . 743 747 + DdeI CTNAG 743 746 . . 743 747 + BspCNI CTCAG 756 754 . . 743 747 + BseMII CTCAG 757 755 . . 748 751 + MseI TTAA 748 750 . . 758 761 + MnlI CCTC 768 767 . . 766 770 + BccI CCATC 774 775 . . 770 774 - FokI GGATG 756 760 . . 770 774 - BseGI GGATG 769 767 . . 785 788 + SetI ASST 788 784 . . 785 791 + PpuMI RGGWCCY 786 789 . . 785 791 + DraII RGGNCCY 786 789 . . 786 791 + NlaIV GGNNCC 788 788 . . 786 790 + BmgT120I GGNCC 787 788 . . 786 790 + AvaII GGWCC 786 789 . . 786 790 + AsuI* GGNCC 786 789 . . 787 791 - BslFI GGGAC 772 776 . . 794 798 + TspDTI ATGAA 809 807 . . 800 803 + SetI ASST 803 799 . . 801 805 + HphI GGTGA 813 812 . . 806 811 + TatI WGTACW 806 810 . . 806 811 + ScaI AGTACT 808 808 . . 807 810 + Csp6I GTAC 807 809 . . 807 810 + RsaI GTAC 808 808 . . 822 827 + AsuII TTCGAA 823 825 . . 823 826 + TaqI TCGA 823 825 . . 831 835 + BlsI GCNGC 833 832 . . 831 835 + BisI GCNGC 832 833 . . 831 835 + BbvI GCAGC 843 847 . . 831 835 + TseI GCWGC 831 834 . . 833 836 + SetI ASST 836 832 . . 833 836 + CviJI RGCY 834 834 . . 833 836 + AluI AGCT 834 834 . . 841 846 + VspI ATTAAT 842 844 . . 842 845 + MseI TTAA 842 844 . . 856 860 + TspDTI ATGAA 871 869 . . 876 879 + SetI ASST 879 875 . . 876 879 + CviJI RGCY 877 877 . . 876 879 + AluI AGCT 877 877 . . 880 885 + VspI ATTAAT 881 883 . . 881 884 + MseI TTAA 881 883 . . 886 890 + HgaI GACGC 895 900 . . 889 894 + NspI RCATGY 893 889 . . 890 893 + FatI CATG 889 893 . . 890 893 + NlaIII CATG 893 889 . . 890 893 + CviAII CATG 890 892 . . 897 901 + BmgT120I GGNCC 898 899 . . 897 901 + AvaII GGWCC 897 900 . . 897 901 + AsuI* GGNCC 897 900 . . 895 898 - AciI CCGC 895 897 . . 899 902 + SetI ASST 902 898 . . 901 905 + DdeI CTNAG 901 904 . . 916 919 - MnlI CCTC 909 908 . . 919 923 + FokI GGATG 932 936 . . 919 923 + BseGI GGATG 925 923 . . 950 955 + AcyI GRCGYC 951 953 . . 951 955 - HgaI GACGC 940 945 . . 960 965 + Hpy178III* TCNNGA 961 963 . . 967 970 + TaiI ACGT 970 966 . . 967 970 + SetI ASST 970 966 . . 967 970 + MaeII ACGT 967 969 . . 972 975 + SetI ASST 975 971 . . 972 975 + CviJI RGCY 973 973 . . 972 975 + AluI AGCT 973 973 . . 976 980 + DdeI CTNAG 976 979 . . 976 980 + BspCNI CTCAG 989 987 . . 976 980 + BseMII CTCAG 990 988 . . 977 981 + Hpy188I TCNGA 979 978 . . 994 998 + Hpy188I TCNGA 996 995 . . 998 1003 + ApoI RAATTY 998 1002 . . 999 1002 + TspEI AATT 998 1002 . . 1002 1007 + Hpy178III* TCNNGA 1003 1005 . . 1012 1016 + MboII GAAGA 1024 1023 . . 1025 1031 + PasI CCCWGGG 1026 1029 . . 1025 1030 + SecI* CCNNGG 1025 1029 . . 1026 1030 + BssKI CCNGG 1025 1030 . . 1026 1030 + BseBI CCWGG 1027 1028 . . 1026 1031 + SecI* CCNNGG 1026 1030 . . 1026 1030 + ScrFI CCNGG 1027 1028 . . 1026 1030 + EcoRII CCWGG 1025 1030 . . 1043 1048 + ApoI RAATTY 1043 1047 . . 1044 1047 + TspEI AATT 1043 1047 . . 1049 1053 - BslFI GGGAC 1034 1038 . . 1061 1068 + PspXI VCTCGAGB 1062 1066 . . 1061 1064 + MnlI CCTC 1071 1070 . . 1061 1068 + AbsI CCTCGAGG 1062 1066 . . 1062 1067 + SmlI CTYRAG 1062 1066 . . 1062 1067 + XhoI CTCGAG 1062 1066 . . 1062 1067 + BmeT110I CYCGRG 1063 1065 . . 1062 1067 + AvaI CYCGRG 1062 1066 . . 1063 1066 + TaqI TCGA 1063 1065 . . 1065 1068 - MnlI CCTC 1058 1057 . . 1069 1072 - MnlI CCTC 1062 1061 . . 1084 1087 - AciI CCGC 1084 1086 . . 1088 1091 + SetI ASST 1091 1087 . . 1087 1090 - MnlI CCTC 1080 1079 . . 1093 1097 + Tsp45I GTSAC 1092 1097 . . 1093 1097 + MaeIII GTNAC 1092 1097 . . 1120 1124 + Hpy188I TCNGA 1122 1121 . . 1121 1125 + DdeI CTNAG 1121 1124 . . 1123 1127 + PleI GAGTC 1131 1132 . . 1123 1127 + MlyI GAGTC 1132 1132 . . 1123 1127 + HinfI GANTC 1123 1126 . . 1125 1130 + Hpy166II GTNNAC 1127 1127 . . 1121 1125 - BspCNI CTCAG 1113 1111 . . 1121 1125 - BseMII CTCAG 1112 1110 . . 1140 1144 - MboII GAAGA 1132 1131 . . 1139 1144 - BbvII* GAAGAC 1132 1136 . . 1151 1154 + SetI ASST 1154 1150 . . 1158 1163 + ApoI RAATTY 1158 1162 . . 1159 1162 + TspEI AATT 1158 1162 . . 1163 1168 + SfeI* CTRYAG 1163 1167 . . 1170 1173 + TspEI AATT 1169 1173 . . 1176 1187 + BdaI TGANNNNNNTCA 1165 1163 1199 1197 1182 1185 + CviJI RGCY 1183 1183 . . 1180 1183 - MnlI CCTC 1173 1172 . . 1176 1187 - BdaI TGANNNNNNTCA 1199 1197 1165 1163 1189 1192 + TspEI AATT 1188 1192 . . 1206 1211 + ApoI RAATTY 1206 1210 . . 1207 1210 + TspEI AATT 1206 1210 . . 1220 1223 + MnlI CCTC 1230 1229 . . 1223 1227 + DdeI CTNAG 1223 1226 . . 1223 1227 + BspCNI CTCAG 1236 1234 . . 1223 1227 + BseMII CTCAG 1237 1235 . . 1224 1228 + Hpy188I TCNGA 1226 1225 . . 1226 1231 + XhoII RGATCY 1226 1230 . . 1226 1231 + BglII AGATCT 1226 1230 . . 1227 1230 + DpnI GATC 1228 1228 . . 1227 1230 + BstKTI GATC 1229 1227 . . 1227 1230 + MboI GATC 1226 1230 . . 1233 1238 - MmeI TCCRAC 1214 1212 . . 1243 1246 + MseI TTAA 1243 1245 . . 1249 1252 + TspEI AATT 1248 1252 . . 1267 1271 + MaeIII GTNAC 1266 1271 . . 1282 1287 + ApoI RAATTY 1282 1286 . . 1283 1286 + TspEI AATT 1282 1286 . . 1286 1289 + MseI TTAA 1286 1288 . . 1292 1296 + BssKI CCNGG 1291 1296 . . 1292 1296 + BseBI CCWGG 1293 1294 . . 1292 1296 + ScrFI CCNGG 1293 1294 . . 1292 1296 + EcoRII CCWGG 1291 1296 . . 1295 1298 + CviJI RGCY 1296 1296 . . 1303 1307 + BssKI CCNGG 1302 1307 . . 1303 1307 + ScrFI CCNGG 1304 1305 . . 1303 1307 + CauII* CCSGG 1304 1305 . . 1304 1307 + HpaII CCGG 1304 1306 . . 1307 1311 + MaeIII GTNAC 1306 1311 . . 1324 1327 + MseI TTAA 1324 1326 . . 1328 1331 + SetI ASST 1331 1327 . . 1328 1331 + CviJI RGCY 1329 1329 . . 1328 1331 + AluI AGCT 1329 1329 . . 1333 1336 + TspEI AATT 1332 1336 . . 1347 1351 + HinfI GANTC 1347 1350 . . 1350 1354 + Hpy188I TCNGA 1352 1351 . . 1347 1351 - PleI GAGTC 1341 1342 . . 1347 1351 - MlyI GAGTC 1341 1341 . . 1353 1357 + TfiI GAWTC 1353 1356 . . 1353 1357 + HinfI GANTC 1353 1356 . . 1358 1361 + TspEI AATT 1357 1361 . . 1367 1370 + MseI TTAA 1367 1369 . . 1371 1376 + ApoI RAATTY 1371 1375 . . 1372 1375 + TspEI AATT 1371 1375 . . 1376 1381 + Hpy178III* TCNNGA 1377 1379 . . 1380 1384 + MboII GAAGA 1392 1391 . . 1398 1403 + XhoII RGATCY 1398 1402 . . 1399 1402 + DpnI GATC 1400 1400 . . 1399 1402 + BstKTI GATC 1401 1399 . . 1399 1402 + MboI GATC 1398 1402 . . 1399 1403 - BinI* GGATC 1393 1394 . . 1413 1418 + Hpy166II GTNNAC 1415 1415 . . 1424 1428 + MaeIII GTNAC 1423 1428 . . 1429 1434 + ClaI ATCGAT 1430 1432 . . 1430 1433 + TaqI TCGA 1430 1432 . . 1445 1449 - TspDTI ATGAA 1435 1433 . . 1471 1475 + BsmAI GTCTC 1476 1480 . . 1479 1482 + TspEI AATT 1478 1482 . . 1491 1494 + TspEI AATT 1490 1494 . . 1496 1501 + AflIII ACRYGT 1496 1500 . . 1497 1502 + BsaAI YACGTR 1499 1499 . . 1498 1501 + TaiI ACGT 1501 1497 . . 1498 1501 + SetI ASST 1501 1497 . . 1498 1501 + MaeII ACGT 1498 1500 . . 1503 1508 + TsoI TARCCA 1519 1517 . . 1506 1510 + BssKI CCNGG 1505 1510 . . 1506 1510 + BseBI CCWGG 1507 1508 . . 1506 1510 + ScrFI CCNGG 1507 1508 . . 1506 1510 + EcoRII CCWGG 1505 1510 . . 1519 1522 - MnlI CCTC 1512 1511 . . 1522 1526 + MboII GAAGA 1534 1533 . . 1525 1529 + TfiI GAWTC 1525 1528 . . 1525 1529 + HinfI GANTC 1525 1528 . . 1519 1529 - Hin4I GAYNNNNNVTC 1542 1537 1510 1505 1538 1542 + TspDTI ATGAA 1553 1551 . . 1576 1580 - MboII GAAGA 1568 1567 . . 1580 1584 + DdeI CTNAG 1580 1583 . . 1580 1584 + BspCNI CTCAG 1593 1591 . . 1580 1584 + BseMII CTCAG 1594 1592 . . 1583 1586 + CviJI RGCY 1584 1584 . . 1598 1601 + SetI ASST 1601 1597 . . 1607 1610 + TspEI AATT 1606 1610 . . 1626 1631 + ApoI RAATTY 1626 1630 . . 1627 1630 + TspEI AATT 1626 1630 . . 1632 1637 + ZraI GACGTC 1634 1634 . . 1632 1637 + AcyI GRCGYC 1633 1635 . . 1632 1637 + AatII GACGTC 1636 1632 . . 1633 1636 + TaiI ACGT 1636 1632 . . 1633 1636 + SetI ASST 1636 1632 . . 1633 1636 + MaeII ACGT 1633 1635 . . 1636 1640 + Hpy188I TCNGA 1638 1637 . . 1639 1643 - Hin4II* CCTTC 1633 1632 . . 1646 1649 + TspEI AATT 1645 1649 . . 1663 1666 + TspEI AATT 1662 1666 . . 1676 1681 + TatI WGTACW 1676 1680 . . 1677 1680 + Csp6I GTAC 1677 1679 . . 1677 1680 + RsaI GTAC 1678 1678 . . 1679 1684 + NspI RCATGY 1683 1679 . . 1680 1683 + FatI CATG 1679 1683 . . 1680 1683 + NlaIII CATG 1683 1679 . . 1680 1683 + CviAII CATG 1680 1682 . . 1686 1691 + XbaI TCTAGA 1686 1690 . . 1686 1691 + Hpy178III* TCNNGA 1687 1689 . . 1687 1690 + MaeI CTAG 1687 1689 . . 1695 1705 + FalI AAGNNNNNCTT 1686 1681 1718 1713 1697 1702 + Hpy166II GTNNAC 1699 1699 . . 1701 1704 + SetI ASST 1704 1700 . . 1695 1705 - FalI AAGNNNNNCTT 1718 1713 1686 1681 1708 1712 + PleI GAGTC 1716 1717 . . 1708 1712 + MlyI GAGTC 1717 1717 . . 1708 1712 + HinfI GANTC 1708 1711 . . 1743 1746 + CviRI* TGCA 1744 1744 . . 1746 1749 + SetI ASST 1749 1745 . . 1744 1749 - BspMI ACCTGC 1735 1739 . . 1750 1754 + BslFI GGGAC 1764 1768 . . 1744 1750 - AarI CACCTGC 1735 1739 . . 1758 1763 + NlaIV GGNNCC 1760 1760 . . 1764 1767 + FatI CATG 1763 1767 . . 1764 1767 + NlaIII CATG 1767 1763 . . 1764 1767 + CviAII CATG 1764 1766 . . 1768 1772 + Hpy188I TCNGA 1770 1769 . . 1780 1783 + TaqI TCGA 1780 1782 . . 1771 1781 - Hin4I GAYNNNNNVTC 1794 1789 1762 1757 1782 1787 + ApoI RAATTY 1782 1786 . . 1783 1786 + TspEI AATT 1782 1786 . . 1791 1794 + SetI ASST 1794 1790 . . 1793 1798 + Eco57MI CTGRAG 1814 1812 . . 1793 1798 + Eco57I CTGAAG 1814 1812 . . 1801 1805 + Hin4II* CCTTC 1811 1810 . . 1804 1808 + Hpy188I TCNGA 1806 1805 . . 1805 1810 + Eco57MI CTGRAG 1826 1824 . . 1805 1810 + Eco57I CTGAAG 1826 1824 . . 1807 1811 + MboII GAAGA 1819 1818 . . 1807 1812 + BbvII* GAAGAC 1814 1818 . . 1818 1826 + Tth111I GACNNNGTC 1821 1822 . . 1819 1822 + SetI ASST 1822 1818 . . 1828 1831 + CviRI* TGCA 1829 1829 . . 1834 1837 + FatI CATG 1833 1837 . . 1834 1837 + NlaIII CATG 1837 1833 . . 1834 1837 + CviAII CATG 1834 1836 . . 1851 1856 + ApoI RAATTY 1851 1855 . . 1852 1855 + TspEI AATT 1851 1855 . . 1856 1859 + CviRI* TGCA 1857 1857 . . 1877 1880 + CviJI RGCY 1878 1878 . . 1879 1885 + Bpu10I CCTNAGC 1880 1883 . . 1880 1884 + DdeI CTNAG 1880 1883 . . 1883 1886 + SetI ASST 1886 1882 . . 1883 1886 + CviJI RGCY 1884 1884 . . 1883 1886 + AluI AGCT 1884 1884 . . 1880 1884 - BspCNI CTCAG 1872 1870 . . 1880 1884 - BseMII CTCAG 1871 1869 . . 1885 1890 + SmlI CTYRAG 1885 1889 . . 1885 1890 + Bce83I* CTTGAG 1906 1904 . . 1890 1895 + Hpy166II GTNNAC 1892 1892 . . 1882 1893 - PpiI GAACNNNNNCTC 1905 1900 1873 1868 1897 1902 + AsuII TTCGAA 1898 1900 . . 1898 1901 + TaqI TCGA 1898 1900 . . 1911 1915 + BsmAI GTCTC 1916 1920 . . 1916 1919 + MnlI CCTC 1926 1925 . . 1943 1946 + TspEI AATT 1942 1946 . . 1954 1957 + DpnI GATC 1955 1955 . . 1954 1957 + BstKTI GATC 1956 1954 . . 1954 1957 + MboI GATC 1953 1957 . . 1956 1960 + Hpy188I TCNGA 1958 1957 . . 1957 1961 + DdeI CTNAG 1957 1960 . . 1957 1961 - BspCNI CTCAG 1949 1947 . . 1957 1961 - BseMII CTCAG 1948 1946 . . 1972 1982 + BsiYI* CCNNNNNNNGG 1978 1975 . . 1992 1995 + SetI ASST 1995 1991 . . 1992 1995 + CviJI RGCY 1993 1993 . . 1992 1995 + AluI AGCT 1993 1993 . . 2005 2008 + SetI ASST 2008 2004 . . 2005 2008 + CviJI RGCY 2006 2006 . . 2005 2008 + AluI AGCT 2006 2006 . . 2020 2023 + Hin6I GCGC 2020 2022 . . 2020 2023 + GlaI GCGC 2021 2021 . . 2020 2023 + HhaI GCGC 2022 2020 . . 2022 2027 + NspI RCATGY 2026 2022 . . 2023 2026 + FatI CATG 2022 2026 . . 2023 2026 + NlaIII CATG 2026 2022 . . 2023 2026 + CviAII CATG 2023 2025 . . 2028 2031 + TspEI AATT 2027 2031 . . 2032 2038 + TspRI CASTGNN 2038 2029 . . 2053 2056 + SetI ASST 2056 2052 . . 2065 2068 + MseI TTAA 2065 2067 . . 2072 2075 + TaiI ACGT 2075 2071 . . 2072 2075 + SetI ASST 2075 2071 . . 2072 2075 + MaeII ACGT 2072 2074 . . 2076 2079 - MnlI CCTC 2069 2068 . . 2079 2088 + XmnI GAANNNNTTC 2083 2083 . . 2082 2087 + SspI AATATT 2084 2084 . . 2090 2094 + TspDTI ATGAA 2105 2103 . . 2102 2106 + BceAI ACGGC 2118 2120 . . 2105 2110 + BtgZI GCGATG 2120 2124 . . 2110 2120 + Hin4I GAYNNNNNVTC 2101 2096 2133 2128 2119 2124 + Hpy178III* TCNNGA 2120 2122 . . 2110 2120 - Hin4I GAYNNNNNVTC 2133 2128 2101 2096 2128 2132 + Hpy188I TCNGA 2130 2129 . . 2131 2135 + TfiI GAWTC 2131 2134 . . 2131 2135 + HinfI GANTC 2131 2134 . . 2143 2146 - MnlI CCTC 2136 2135 . . 2147 2151 - Hin4II* CCTTC 2141 2140 . . 2153 2158 + Eco31I GGTCTC 2159 2163 . . 2154 2158 + BsmAI GTCTC 2159 2163 . . 2163 2168 + Cac8I GCNNGC 2165 2165 . . 2166 2169 + CviJI RGCY 2167 2167 . . 2165 2170 - TsoI TARCCA 2155 2153 . . 2183 2186 + TspEI AATT 2182 2186 . . 2188 2192 + Hpy188I TCNGA 2190 2189 . . 2191 2194 - MnlI CCTC 2184 2183 . . 2199 2204 + ApoI RAATTY 2199 2203 . . 2200 2203 + TspEI AATT 2199 2203 . . 2205 2210 + Hpy178III* TCNNGA 2206 2208 . . 2223 2226 + CviJI RGCY 2224 2224 . . 2229 2233 + HphI GGTGA 2241 2240 . . 2232 2236 - BccI CCATC 2226 2227 . . 2238 2242 + BmgT120I GGNCC 2239 2240 . . 2238 2242 + AsuI* GGNCC 2238 2241 . . 2239 2242 + CviJI RGCY 2240 2240 . . 2239 2242 + HaeIII GGCC 2240 2240 . . 2240 2245 + Cac8I GCNNGC 2242 2242 . . 2244 2248 - BceAI ACGGC 2229 2231 . . 2254 2259 + SspI AATATT 2256 2256 . . 2279 2282 + MseI TTAA 2279 2281 . . 2294 2299 + CfrI YGGCCR 2294 2298 . . 2295 2298 + CviJI RGCY 2296 2296 . . 2295 2298 + HaeIII GGCC 2296 2296 . . 2296 2300 + TauI GCSGC 2299 2296 . . 2296 2300 + BlsI GCNGC 2298 2297 . . 2296 2300 + BisI GCNGC 2297 2298 . . 2297 2300 + AciI CCGC 2297 2299 . . 2308 2312 + TspDTI ATGAA 2323 2321 . . 2317 2322 + EciI GGCGGA 2333 2331 . . 2318 2321 - AciI CCGC 2318 2320 . . 2323 2327 + TspGWI ACGGA 2338 2336 . . 2326 2330 + MboII GAAGA 2338 2337 . . 2326 2331 - Ksp632I* CTCTTC 2321 2324 . . 2329 2332 - MnlI CCTC 2322 2321 . . 2333 2338 + HindIII AAGCTT 2333 2337 . . 2334 2337 + SetI ASST 2337 2333 . . 2334 2337 + CviJI RGCY 2335 2335 . . 2334 2337 + AluI AGCT 2335 2335 . . 2335 2345 + MwoI GCNNNNNNNGC 2341 2338 . . 2338 2343 + Hpy178III* TCNNGA 2339 2341 . . 2339 2344 + BsiI* CACGAG 2339 2343 . . 2365 2370 + SspI AATATT 2367 2367 . . 2374 2378 + TspDTI ATGAA 2389 2387 . . 2375 2386 + BdaI TGANNNNNNTCA 2364 2362 2398 2396 2379 2382 + SetI ASST 2382 2378 . . 2379 2382 + CviJI RGCY 2380 2380 . . 2379 2382 + AluI AGCT 2380 2380 . . 2383 2388 + Hpy166II GTNNAC 2385 2385 . . 2383 2388 + HindII GTYRAC 2385 2385 . . 2375 2386 - BdaI TGANNNNNNTCA 2398 2396 2364 2362 2388 2399 + BstXI CCANNNNNNTGG 2395 2391 . . 2400 2403 + TaiI ACGT 2403 2399 . . 2400 2403 + SetI ASST 2403 2399 . . 2395 2400 - MmeI TCCRAC 2376 2374 . . 2400 2403 + MaeII ACGT 2400 2402 . . 2399 2404 - BtrI CACGTC 2401 2401 . . 2409 2413 + BceAI ACGGC 2425 2427 . . 2412 2417 + NspI RCATGY 2416 2412 . . 2412 2417 + Cac8I GCNNGC 2414 2414 . . 2412 2417 + SphI GCATGC 2416 2412 . . 2413 2422 + MslI CAYNNNNRTG 2417 2417 . . 2413 2416 + FatI CATG 2412 2416 . . 2413 2416 + NlaIII CATG 2416 2412 . . 2413 2416 + CviAII CATG 2413 2415 . . 2415 2418 + CviRI* TGCA 2416 2416 . . 2442 2452 + MwoI GCNNNNNNNGC 2448 2445 . . 2444 2447 + TaqI TCGA 2444 2446 . . 2462 2465 + CviJI RGCY 2463 2463 . . 2462 2465 + HaeIII GGCC 2463 2463 . . 2462 2466 + BmgT120I GGNCC 2463 2464 . . 2462 2466 + AsuI* GGNCC 2462 2465 . . 2465 2475 + BsiYI* CCNNNNNNNGG 2471 2468 . . 2467 2470 + TspEI AATT 2466 2470 . . 2471 2476 + Hpy178III* TCNNGA 2472 2474 . . 2471 2476 + BetI* WCCGGW 2471 2475 . . 2471 2476 + BspMII* TCCGGA 2471 2475 . . 2472 2475 + HpaII CCGG 2472 2474 . . 2480 2483 + TspEI AATT 2479 2483 . . 2484 2488 + Tsp45I GTSAC 2483 2488 . . 2484 2488 + MaeIII GTNAC 2483 2488 . . 2487 2490 + TaiI ACGT 2490 2486 . . 2487 2490 + SetI ASST 2490 2486 . . 2487 2490 + MaeII ACGT 2487 2489 . . 2495 2498 + CviRI* TGCA 2496 2496 . . 2491 2496 - TaqII CACCCA 2481 2479 . . 2500 2504 + TfiI GAWTC 2500 2503 . . 2500 2504 + HinfI GANTC 2500 2503 . . 2509 2512 + TspEI AATT 2508 2512 . . 2512 2516 + Hpy188I TCNGA 2514 2513 . . 2531 2534 + SetI ASST 2534 2530 . . 2539 2543 - MboII GAAGA 2531 2530 . . 2547 2551 + TspGWI ACGGA 2562 2560 . . 2564 2569 + MstI* TGCGCA 2566 2566 . . 2565 2568 + Hin6I GCGC 2565 2567 . . 2565 2568 + GlaI GCGC 2566 2566 . . 2565 2568 + HhaI GCGC 2567 2565 . . 2567 2571 + BlsI GCNGC 2569 2568 . . 2567 2571 + BisI GCNGC 2568 2569 . . 2567 2571 + BbvI GCAGC 2579 2583 . . 2567 2571 + TseI GCWGC 2567 2570 . . 2569 2572 + CviJI RGCY 2570 2570 . . 2574 2579 + EcoRI GAATTC 2574 2578 . . 2574 2579 + ApoI RAATTY 2574 2578 . . 2575 2578 + TspEI AATT 2574 2578 . . #--------------------------------------- # # # AarI: # [CACCTGC] # 851 1735 # # AatII: # [GACGTC] # 950 1636 # # AbsI: # [CCTCGAGG] # 1062 1524 # # AccI: # [GTMKAC] # 80 2506 # # AciI: # [CCGC] # 21 189 207 268 292 396 # 1213 # # AcyI: # [GRCGYC] # 682 951 953 # # AflIII: # [ACRYGT] # 1090 1496 # # AhaIII*: # [TTTAAA] # 347 2239 # # AluI: # [AGCT] # 13 43 45 96 109 112 # 206 329 356 555 722 # # AlwNI: # [CAGNNNCTG] # 520 2066 # # ApoI: # [RAATTY] # 12 45 48 69 76 89 # 115 156 255 348 375 404 # 594 # # AsuI*: # [GGNCC] # 111 124 224 786 1341 # # AsuII: # [TTCGAA] # 688 823 1075 # # AvaI: # [CYCGRG] # 1062 1524 # # AvaII: # [GGWCC] # 111 786 1689 # # BbvI: # [GCAGC] # 7 843 1736 # # BbvII*: # [GAAGAC] # 682 772 1132 # # BccI: # [CCATC] # 152 360 622 1452 # # Bce83I*: # [CTTGAG] # 680 1906 # # BceAI: # [ACGGC] # 111 161 196 2118 # # BdaI: # [TGANNNNNNTCA] # 0 0 0 0 34 34 # 188 1165 1165 # # BetI*: # [WCCGGW] # 115 2471 # # BfiI: # [ACTGGG] # 140 142 2304 # # BglII: # [AGATCT] # 1226 1360 # # BinI*: # [GGATC] # 216 424 753 1193 # # BisI: # [GCNGC] # 18 271 832 1465 # # BlsI: # [GCNGC] # 17 271 833 1465 # # BmeT110I: # [CYCGRG] # 1063 1523 # # BmgT120I: # [GGNCC] # 111 123 224 787 1341 # # Bpu10I: # [CCTNAGC] # 253 706 1627 # # BsaAI: # [YACGTR] # 1087 1499 # # BsaBI: # [GATNNNNATC] # 420 2166 # # BseBI: # [CCWGG] # 118 214 266 909 1079 # # BseGI: # [GGATG] # 33 63 156 673 1661 # # BseMII: # [CTCAG] # 77 122 125 233 277 357 # 638 757 # # BseRI: # [GAGGAG] # 566 2020 # # BsiI*: # [CACGAG] # 247 2339 # # BsiYI*: # [CCNNNNNNNGG] # 115 493 655 1323 # # BslFI: # [GGGAC] # 147 262 625 730 822 # # BsmAI: # [GTCTC] # 16 243 427 440 1460 # # BspCNI: # [CTCAG] # 77 123 124 233 279 357 # 637 756 # # BspMI: # [ACCTGC] # 851 1735 # # BspMII*: # [TCCGGA] # 115 2471 # # BsrI: # [ACTGG] # 88 135 142 323 1898 # # BssKI: # [CCNGG] # 11 116 203 266 909 1081 # # BssNAI: # [GTATAC] # 81 2505 # # BstKTI: # [GATC] # 15 172 216 432 555 566 # 630 # # BstXI: # [CCANNNNNNTGG] # 191 2395 # # BtgZI: # [GCGATG] # 272 466 1848 # # BtrI: # [CACGTC] # 185 2401 # # Cac8I: # [GCNNGC] # 77 172 172 2165 # # CauII*: # [CCSGG] # 1282 1304 # # CfrI: # [YGGCCR] # 292 2294 # # ClaI: # [ATCGAT] # 1156 1430 # # Csp6I: # [GTAC] # 61 300 446 870 909 # # CviAII: # [CATG] # 70 84 173 189 199 210 # 390 481 790 # # CviJI: # [RGCY] # 6 13 13 16 16 33 # 39 43 45 45 56 57 # 67 83 96 107 109 113 # 142 161 210 255 294 567 # # CviRI*: # [TGCA] # 28 80 85 90 559 706 # 1038 # # DdeI: # [CTNAG] # 75 77 102 145 158 253 # 300 357 490 629 # # DpnI: # [GATC] # 15 172 216 431 555 566 # 631 # # DraII: # [RGGNCCY] # 786 1800 # # EciI: # [GGCGGA] # 253 2333 # # Eco31I: # [GGTCTC] # 427 2159 # # Eco57I: # [CTGAAG] # 12 760 1814 # # Eco57MI: # [CTGRAG] # 12 760 1814 # # EcoRI: # [GAATTC] # 12 2574 # # EcoRII: # [CCWGG] # 116 214 266 909 1081 # # EcoT22I: # [ATGCAT] # 708 1878 # # FalI: # [AAGNNNNNCTT] # 0 0 32 868 1686 # # FatI: # [CATG] # 70 84 174 189 199 209 # 390 481 790 # # FauI: # [CCCGC] # 214 2372 # # FnuDII*: # [CGCG] # 501 2085 # # FokI: # [GGATG] # 20 63 176 673 1654 # # GlaI: # [GCGC] # 20 46 456 545 1519 # # HaeII: # [RGCGCY] # 458 2128 # # HaeIII: # [GGCC] # 56 123 167 2240 # # HgaI: # [GACGC] # 45 153 742 1646 # # HgiCI*: # [GGYRCC] # 461 2125 # # HhaI: # [GCGC] # 19 46 457 545 1519 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 15 32 # 32 32 32 220 307 453 # 1463 # # Hin4II*: # [CCTTC] # 91 178 330 445 536 1006 # # Hin6I: # [GCGC] # 21 46 455 545 1519 # # HindII: # [GTYRAC] # 201 2385 # # HindIII: # [AAGCTT] # 253 2333 # # HinfI: # [GANTC] # 6 6 86 172 183 224 # 369 423 511 606 # # HpaII: # [CCGG] # 114 1168 1304 # # HphI: # [GGTGA] # 195 345 618 1428 # # Hpy166II: # [GTNNAC] # 81 193 201 284 288 493 # 508 538 # # Hpy178III*: # [TCNNGA] # 42 69 86 114 133 133 # 251 310 374 433 641 # # Hpy188I: # [TCNGA] # 6 17 21 36 45 60 # 72 75 104 126 126 132 # 152 172 286 324 412 420 # # Ksp632I*: # [CTCTTC] # 265 2321 # # MaeI: # [CTAG] # 458 899 1229 # # MaeII: # [ACGT] # 87 99 135 296 328 439 # 531 671 # # MaeIII: # [GTNAC] # 40 103 117 174 420 672 # 1060 # # MboI: # [GATC] # 15 172 216 429 555 566 # 633 # # MboII: # [GAAGA] # 34 55 68 76 108 114 # 133 142 178 193 251 260 # 455 519 # # McrI*: # [CGRYCG] # 663 1923 # # MlyI: # [GAGTC] # 209 376 869 1132 # # MmeI: # [TCCRAC] # 210 1162 1214 # # MnlI: # [CCTC] # 1 2 4 9 9 48 # 57 67 93 138 141 143 # 149 221 264 282 414 544 # # MseI: # [TTAA] # 10 38 39 43 43 67 # 80 87 94 192 214 307 # 312 362 698 # # MslI: # [CAYNNNNRTG] # 169 208 259 1950 # # MstI*: # [TGCGCA] # 20 2566 # # MwoI: # [GCNNNNNNNGC] # 107 138 2341 # # NlaIII: # [CATG] # 70 84 170 189 199 213 # 390 481 790 # # NlaIV: # [GGNNCC] # 325 463 826 972 # # NspI: # [RCATGY] # 170 343 390 790 893 # # PasI: # [CCCWGGG] # 1026 1560 # # PflMI: # [CCANNNNNTGG] # 655 1931 # # PfoI: # [TCCNGGA] # 116 2470 # # PleI: # [GAGTC] # 210 375 870 1131 # # PpiI: # [GAACNNNNNCTC] # 32 681 1873 # # PpuMI: # [RGGWCCY] # 786 1800 # # PsiI: # [TTATAA] # 41 2545 # # PspXI: # [VCTCGAGB] # 1062 1524 # # PvuI: # [CGATCG] # 663 1923 # # RsaI: # [GTAC] # 62 300 446 870 908 # # ScaI: # [AGTACT] # 808 1778 # # ScrFI: # [CCNGG] # 11 118 203 266 909 1079 # # SecI*: # [CCNNGG] # 1 1025 1560 # # SetI: # [ASST] # 5 13 15 19 21 23 # 28 33 35 36 43 44 # 45 45 45 48 52 52 # 56 58 62 63 63 64 # 68 68 71 87 100 100 # 109 116 140 150 170 177 # 262 # # SfaNI: # [GCATC] # 715 1871 # # SfeI*: # [CTRYAG] # 1163 1423 # # SgfI: # [GCGATCGC] # 663 1923 # # SmlI: # [CTYRAG] # 701 823 1062 # # SphI: # [GCATGC] # 170 2416 # # SplI*: # [CGTACG] # 506 2080 # # SspI: # [AATATT] # 16 111 172 219 2068 # # TaiI: # [ACGT] # 87 96 135 296 328 439 # 531 674 # # TaqI: # [TCGA] # 118 142 183 240 291 349 # 350 367 546 # # TaqII: # [CACCCA] # 105 2481 # # TatI: # [WGTACW] # 806 870 910 # # TauI: # [GCSGC] # 287 2299 # # TfiI: # [GAWTC] # 6 86 172 369 511 606 # 836 # # TseI: # [GCWGC] # 19 831 1736 # # TsoI: # [TARCCA] # 431 636 1519 # # Tsp45I: # [GTSAC] # 103 1092 1391 # # Tsp4CI*: # [ACNGT] # 13 65 2508 # # TspDTI: # [ATGAA] # 49 62 66 118 197 199 # 218 552 561 564 # # TspEI: # [AATT] # 9 11 12 12 13 14 # 14 17 17 18 19 19 # 20 25 29 29 34 42 # 45 50 66 69 85 91 # 101 107 115 116 120 155 # 227 265 267 353 # # TspGWI: # [ACGGA] # 24 224 2338 # # TspRI: # [CASTGNN] # 135 548 560 1343 # # Tth111I: # [GACNNNGTC] # 147 765 1674 # # VspI: # [ATTAAT] # 39 842 1705 # # XbaI: # [TCTAGA] # 900 1686 # # XhoI: # [CTCGAG] # 1062 1524 # # XhoII: # [RGATCY] # 172 581 645 1188 # # XmnI: # [GAANNNNTTC] # 503 717 1366 # # ZraI: # [GACGTC] # 952 1634 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 2586 # Reported_sequences: 1 # Reported_hitcount: 567 #---------------------------------------