Restriction Fragments for YORWsigma4



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The currently selected gene/sequence is :

YORWsigma4 on chromosome XV from coordinates 968991 to 969301.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.460.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 9 13 + DdeI CTNAG 9 12 . . 9 13 + BspCNI CTCAG 22 20 . . 9 13 + BseMII CTCAG 23 21 . . 10 14 + Hpy188I TCNGA 12 11 . . 16 27 + BdaI TGANNNNNNTCA 5 3 39 37 18 23 + XhoII RGATCY 18 22 . . 19 22 + DpnI GATC 20 20 . . 19 22 + BstKTI GATC 21 19 . . 19 22 + MboI GATC 18 22 . . 23 26 + MnlI CCTC 33 32 . . 19 23 - BinI* GGATC 13 14 . . 24 28 + DdeI CTNAG 24 27 . . 24 28 + BspCNI CTCAG 37 35 . . 24 28 + BseMII CTCAG 38 36 . . 16 27 - BdaI TGANNNNNNTCA 39 37 5 3 32 42 + Hin4I GAYNNNNNVTC 23 18 55 50 32 42 - Hin4I GAYNNNNNVTC 55 50 23 18 49 54 + AclI AACGTT 50 52 . . 50 53 + TaiI ACGT 53 49 . . 50 53 + SetI ASST 53 49 . . 50 53 + MaeII ACGT 50 52 . . 64 69 + NdeI CATATG 65 67 . . 67 71 + TspDTI ATGAA 82 80 . . 78 83 + PsiI TTATAA 80 80 . . 105 108 + FatI CATG 104 108 . . 105 108 + NlaIII CATG 108 104 . . 105 108 + CviAII CATG 105 107 . . 111 115 + TspDTI ATGAA 126 124 . . 119 122 + AciI CCGC 119 121 . . 120 123 + FnuDII* CGCG 121 121 . . 121 125 - HgaI GACGC 110 115 . . 126 132 + Bpu10I CCTNAGC 127 130 . . 127 131 + DdeI CTNAG 127 130 . . 123 127 - BslFI GGGAC 108 112 . . 130 133 + SetI ASST 133 129 . . 130 133 + CviJI RGCY 131 131 . . 130 133 + AluI AGCT 131 131 . . 145 148 + TaiI ACGT 148 144 . . 145 148 + SetI ASST 148 144 . . 145 148 + MaeII ACGT 145 147 . . 146 151 + SplI* CGTACG 146 150 . . 147 150 + Csp6I GTAC 147 149 . . 147 150 + RsaI GTAC 148 148 . . 151 156 + BsmI GAATGC 157 155 . . 163 166 + TspEI AATT 162 166 . . 172 177 + XhoII RGATCY 172 176 . . 173 176 + DpnI GATC 174 174 . . 173 176 + BstKTI GATC 175 173 . . 173 176 + MboI GATC 172 176 . . 175 179 + Hpy188I TCNGA 177 176 . . 173 177 - BinI* GGATC 167 168 . . 180 184 + TfiI GAWTC 180 183 . . 180 184 + HinfI GANTC 180 183 . . 184 187 + AciI CCGC 184 186 . . 185 188 + FnuDII* CGCG 186 186 . . 186 189 + Hin6I GCGC 186 188 . . 186 189 + GlaI GCGC 187 187 . . 186 189 + HhaI GCGC 188 186 . . 192 196 + BccI CCATC 200 201 . . 236 240 + MaeIII GTNAC 235 240 . . 241 244 + CviJI RGCY 242 242 . . 242 247 + Cac8I GCNNGC 244 244 . . 243 247 + FauI CCCGC 251 253 . . 244 247 + AciI CCGC 244 246 . . 245 248 + FnuDII* CGCG 246 246 . . 246 256 + MwoI GCNNNNNNNGC 252 249 . . 248 252 + TfiI GAWTC 248 251 . . 248 252 + HinfI GANTC 248 251 . . 254 257 + SetI ASST 257 253 . . 254 257 + CviJI RGCY 255 255 . . 254 257 + AluI AGCT 255 255 . . 263 267 - TspGWI ACGGA 253 251 . . 273 278 + PvuII CAGCTG 275 275 . . 273 278 + NspBII* CMGCKG 275 275 . . 274 277 + SetI ASST 277 273 . . 274 277 + CviJI RGCY 275 275 . . 274 277 + AluI AGCT 275 275 . . #--------------------------------------- # # # AciI: # [CCGC] # 60 65 67 119 # # AclI: # [AACGTT] # 50 261 # # AluI: # [AGCT] # 20 36 124 131 # # BccI: # [CCATC] # 111 200 # # BdaI: # [TGANNNNNNTCA] # 0 0 5 34 272 # # BinI*: # [GGATC] # 13 144 154 # # Bpu10I: # [CCTNAGC] # 127 184 # # BseMII: # [CTCAG] # 15 23 273 # # BslFI: # [GGGAC] # 108 203 # # BsmI: # [GAATGC] # 154 157 # # BspCNI: # [CTCAG] # 15 22 274 # # BstKTI: # [GATC] # 21 136 154 # # Cac8I: # [GCNNGC] # 67 244 # # Csp6I: # [GTAC] # 147 164 # # CviAII: # [CATG] # 105 206 # # CviJI: # [RGCY] # 13 20 36 111 131 # # DdeI: # [CTNAG] # 9 15 103 184 # # DpnI: # [GATC] # 20 137 154 # # FatI: # [CATG] # 104 207 # # FauI: # [CCCGC] # 60 251 # # FnuDII*: # [CGCG] # 60 65 65 121 # # GlaI: # [GCGC] # 124 187 # # HgaI: # [GACGC] # 110 201 # # HhaI: # [GCGC] # 123 188 # # Hin4I: # [GAYNNNNNVTC] # 0 0 23 32 256 # # Hin6I: # [GCGC] # 125 186 # # HinfI: # [GANTC] # 63 68 180 # # Hpy188I: # [TCNGA] # 12 134 165 # # MaeII: # [ACGT] # 50 95 166 # # MaeIII: # [GTNAC] # 76 235 # # MboI: # [GATC] # 18 139 154 # # MnlI: # [CCTC] # 33 278 # # MwoI: # [GCNNNNNNNGC] # 59 252 # # NdeI: # [CATATG] # 65 246 # # NlaIII: # [CATG] # 108 203 # # NspBII*: # [CMGCKG] # 36 275 # # PsiI: # [TTATAA] # 80 231 # # PvuII: # [CAGCTG] # 36 275 # # RsaI: # [GTAC] # 148 163 # # SetI: # [ASST] # 15 20 34 53 80 109 # # SplI*: # [CGTACG] # 146 165 # # TaiI: # [ACGT] # 53 95 163 # # TfiI: # [GAWTC] # 63 68 180 # # TspDTI: # [ATGAA] # 44 82 185 # # TspEI: # [AATT] # 149 162 # # TspGWI: # [ACGGA] # 58 253 # # XhoII: # [RGATCY] # 18 139 154 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 311 # Reported_sequences: 1 # Reported_hitcount: 77 #---------------------------------------