Restriction Fragments for YORCdelta20



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The currently selected gene/sequence is :

YORCdelta20 on chromosome XV from coordinates 704395 to 704063.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.25634.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 31 36 + SpeI ACTAGT 31 35 . . 32 35 + MaeI CTAG 32 34 . . 41 44 + TaiI ACGT 44 40 . . 41 44 + SetI ASST 44 40 . . 41 44 + MaeII ACGT 41 43 . . 43 47 + MaeIII GTNAC 42 47 . . 46 51 + SpeI ACTAGT 46 50 . . 47 50 + MaeI CTAG 47 49 . . 64 68 + Tsp4CI* ACNGT 66 65 . . 73 77 + MboII GAAGA 85 84 . . 79 83 + HgaI GACGC 88 93 . . 91 101 - Hin4I GAYNNNNNVTC 114 109 82 77 107 110 + TspEI AATT 106 110 . . 117 120 + SetI ASST 120 116 . . 117 120 + CviJI RGCY 118 118 . . 117 120 + AluI AGCT 118 118 . . 147 151 + BinI* GGATC 155 156 . . 148 151 + DpnI GATC 149 149 . . 148 151 + BstKTI GATC 150 148 . . 148 151 + MboI GATC 147 151 . . 153 157 + TspDTI ATGAA 168 166 . . 156 161 + SspI AATATT 158 158 . . 160 163 + MseI TTAA 160 162 . . 183 188 + SspI AATATT 185 185 . . 194 197 + TspEI AATT 193 197 . . 204 207 + TspEI AATT 203 207 . . 207 210 + CviRI* TGCA 208 208 . . 211 215 + TfiI GAWTC 211 214 . . 211 215 + HinfI GANTC 211 214 . . 215 225 + BsiYI* CCNNNNNNNGG 221 218 . . 221 224 + FatI CATG 220 224 . . 221 224 + NlaIII CATG 224 220 . . 221 224 + CviAII CATG 221 223 . . 219 223 - TspDTI ATGAA 209 207 . . 225 229 + TfiI GAWTC 225 228 . . 225 229 + HinfI GANTC 225 228 . . 237 242 + SmlI CTYRAG 237 241 . . 237 242 + Bce83I* CTTGAG 258 256 . . 240 245 + BseRI GAGGAG 255 253 . . 240 243 - MnlI CCTC 233 232 . . 245 257 + PsrI GAACNNNNNNTAC 237 232 269 264 251 254 + MaeI CTAG 251 253 . . 253 258 + TatI WGTACW 253 257 . . 254 257 + Csp6I GTAC 254 256 . . 254 257 + RsaI GTAC 255 255 . . 267 270 + SetI ASST 270 266 . . 271 276 + SspI AATATT 273 273 . . 286 289 + MseI TTAA 286 288 . . 295 299 + TfiI GAWTC 295 298 . . 295 299 + HinfI GANTC 295 298 . . 305 308 + TspEI AATT 304 308 . . 315 320 + ApoI RAATTY 315 319 . . 316 319 + TspEI AATT 315 319 . . 319 323 - HphI GGTGA 311 310 . . #--------------------------------------- # # # AluI: # [AGCT] # 118 215 # # ApoI: # [RAATTY] # 18 315 # # Bce83I*: # [CTTGAG] # 75 258 # # BinI*: # [GGATC] # 155 178 # # BseRI: # [GAGGAG] # 78 255 # # BsiYI*: # [CCNNNNNNNGG] # 112 221 # # BstKTI: # [GATC] # 150 183 # # Csp6I: # [GTAC] # 79 254 # # CviAII: # [CATG] # 112 221 # # CviJI: # [RGCY] # 118 215 # # CviRI*: # [TGCA] # 125 208 # # DpnI: # [GATC] # 149 184 # # FatI: # [CATG] # 113 220 # # HgaI: # [GACGC] # 88 245 # # Hin4I: # [GAYNNNNNVTC] # 32 82 219 # # HinfI: # [GANTC] # 14 38 70 211 # # HphI: # [GGTGA] # 22 311 # # MaeI: # [CTAG] # 15 32 82 204 # # MaeII: # [ACGT] # 41 292 # # MaeIII: # [GTNAC] # 42 291 # # MboI: # [GATC] # 147 186 # # MboII: # [GAAGA] # 85 248 # # MnlI: # [CCTC] # 100 233 # # MseI: # [TTAA] # 47 126 160 # # NlaIII: # [CATG] # 109 224 # # PsrI: # [GAACNNNNNNTAC] # 32 64 237 # # RsaI: # [GTAC] # 78 255 # # SetI: # [ASST] # 44 63 76 150 # # SmlI: # [CTYRAG] # 96 237 # # SpeI: # [ACTAGT] # 15 31 287 # # SspI: # [AATATT] # 27 60 88 158 # # TaiI: # [ACGT] # 44 289 # # TatI: # [WGTACW] # 80 253 # # TfiI: # [GAWTC] # 14 38 70 211 # # Tsp4CI*: # [ACNGT] # 66 267 # # TspDTI: # [ATGAA] # 41 124 168 # # TspEI: # [AATT] # 10 11 18 87 101 106 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 333 # Reported_sequences: 1 # Reported_hitcount: 54 #---------------------------------------