Restriction Fragments for YORCdelta15



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The currently selected gene/sequence is :

YORCdelta15 on chromosome XV from coordinates 664324 to 664003.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.28569.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 5 15 - Hin4I GAYNNNNNVTC 28 23 . . 21 25 - MboII GAAGA 13 12 . . 31 36 + SpeI ACTAGT 31 35 . . 32 35 + MaeI CTAG 32 34 . . 44 47 + MaeI CTAG 44 46 . . 60 65 + NspI RCATGY 64 60 . . 61 64 + FatI CATG 60 64 . . 61 64 + NlaIII CATG 64 60 . . 61 64 + CviAII CATG 61 63 . . 64 68 + TauI GCSGC 67 64 . . 64 68 + BlsI GCNGC 66 65 . . 64 68 + BisI GCNGC 65 66 . . 64 67 - AciI CCGC 64 66 . . 91 101 - Hin4I GAYNNNNNVTC 114 109 82 77 117 120 + SetI ASST 120 116 . . 117 120 + CviJI RGCY 118 118 . . 117 120 + AluI AGCT 118 118 . . 125 128 + CviRI* TGCA 126 126 . . 144 147 + MaeI CTAG 144 146 . . 152 156 + TspDTI ATGAA 167 165 . . 166 170 + TspGWI ACGGA 181 179 . . 169 173 - Hin4II* CCTTC 163 162 . . 173 176 - MnlI CCTC 166 165 . . 173 183 - Hin4I GAYNNNNNVTC 196 191 164 159 186 191 + SspI AATATT 188 188 . . 208 213 + NlaIV GGNNCC 210 210 . . 212 222 + BsiYI* CCNNNNNNNGG 218 215 . . 219 222 - MnlI CCTC 212 211 . . 234 239 + SfeI* CTRYAG 234 238 . . 241 244 - MnlI CCTC 234 233 . . 249 252 + MaeI CTAG 249 251 . . 259 264 + SfeI* CTRYAG 259 263 . . 261 266 + Hpy166II GTNNAC 263 263 . . 261 266 + AccI GTMKAC 262 264 . . 269 274 + SspI AATATT 271 271 . . 277 280 + CviJI RGCY 278 278 . . 277 280 + HaeIII GGCC 278 278 . . 284 289 + Hpy166II GTNNAC 286 286 . . 284 289 + SduI GDGCHC 288 284 . . 284 289 + BseSI GKGCMC 288 284 . . 284 289 + ApaLI GTGCAC 284 288 . . 284 289 + HgiAI* GWGCWC 288 284 . . 285 288 + CviRI* TGCA 286 286 . . 294 299 + XhoII RGATCY 294 298 . . 294 299 + NlaIV GGNNCC 296 296 . . 294 299 + BamHI GGATCC 294 298 . . 294 298 + BinI* GGATC 302 303 . . 295 298 + DpnI GATC 296 296 . . 295 298 + BstKTI GATC 297 295 . . 295 298 + MboI GATC 294 298 . . 295 299 - BinI* GGATC 289 290 . . 304 307 + TspEI AATT 303 307 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 60 262 # # AciI: # [CCGC] # 64 258 # # AluI: # [AGCT] # 118 204 # # ApaLI: # [GTGCAC] # 38 284 # # BamHI: # [GGATCC] # 28 294 # # BinI*: # [GGATC] # 13 20 289 # # BisI: # [GCNGC] # 65 257 # # BlsI: # [GCNGC] # 66 256 # # BseSI: # [GKGCMC] # 34 288 # # BsiYI*: # [CCNNNNNNNGG] # 104 218 # # BstKTI: # [GATC] # 25 297 # # CviAII: # [CATG] # 61 261 # # CviJI: # [RGCY] # 44 118 160 # # CviRI*: # [TGCA] # 36 126 160 # # DpnI: # [GATC] # 26 296 # # FatI: # [CATG] # 60 262 # # HaeIII: # [GGCC] # 44 278 # # HgiAI*: # [GWGCWC] # 34 288 # # Hin4I: # [GAYNNNNNVTC] # 28 32 32 50 54 126 # # Hin4II*: # [CCTTC] # 159 163 # # Hpy166II: # [GTNNAC] # 23 36 263 # # MaeI: # [CTAG] # 12 32 73 100 105 # # MboI: # [GATC] # 28 294 # # MboII: # [GAAGA] # 13 309 # # MnlI: # [CCTC] # 22 46 88 166 # # NlaIII: # [CATG] # 64 258 # # NlaIV: # [GGNNCC] # 26 86 210 # # NspI: # [RCATGY] # 64 258 # # SduI: # [GDGCHC] # 34 288 # # SetI: # [ASST] # 120 202 # # SfeI*: # [CTRYAG] # 25 63 234 # # SpeI: # [ACTAGT] # 31 291 # # SspI: # [AATATT] # 51 83 188 # # TauI: # [GCSGC] # 67 255 # # TspDTI: # [ATGAA] # 155 167 # # TspEI: # [AATT] # 19 303 # # TspGWI: # [ACGGA] # 141 181 # # XhoII: # [RGATCY] # 28 294 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 322 # Reported_sequences: 1 # Reported_hitcount: 52 #---------------------------------------