Restriction Fragments for SRL1/YOR247W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

SRL1/YOR247W on chromosome XV from coordinates 797676 to 798308.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.24790.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 31 34 + MseI TTAA 31 33 . . 34 37 + SetI ASST 37 33 . . 35 39 + Hin4II* CCTTC 45 44 . . 73 77 + Tsp4CI* ACNGT 75 74 . . 96 101 + HaeII RGCGCY 100 96 . . 97 100 + Hin6I GCGC 97 99 . . 97 100 + GlaI GCGC 98 98 . . 97 100 + HhaI GCGC 99 97 . . 100 105 + GsaI CCCAGC 104 100 . . 100 105 + BseYI CCCAGC 100 104 . . 101 115 + XcmI CCANNNNNNNNNTGG 108 107 . . 103 106 + SetI ASST 106 102 . . 103 106 + CviJI RGCY 104 104 . . 103 106 + AluI AGCT 104 104 . . 110 115 + StyI CCWWGG 110 114 . . 110 115 + SecI* CCNNGG 110 114 . . 114 119 + NlaIV GGNNCC 116 116 . . 114 119 + HgiCI* GGYRCC 114 118 . . 115 120 + SduI GDGCHC 119 115 . . 115 120 + BseSI GKGCMC 119 115 . . 124 128 - BsmAI GTCTC 118 122 . . 137 142 + SplI* CGTACG 137 141 . . 138 141 + Csp6I GTAC 138 140 . . 138 141 + RsaI GTAC 139 139 . . 144 148 + Hpy99I CGWCG 148 143 . . 157 160 + SetI ASST 160 156 . . 166 170 + Tsp45I GTSAC 165 170 . . 166 170 + MaeIII GTNAC 165 170 . . 169 172 + SetI ASST 172 168 . . 170 173 + MnlI CCTC 180 179 . . 167 171 - HphI GGTGA 159 158 . . 175 179 + Tsp4CI* ACNGT 177 176 . . 178 183 + Hpy166II GTNNAC 180 180 . . 178 183 + AccI GTMKAC 179 181 . . 185 195 + BsiYI* CCNNNNNNNGG 191 188 . . 185 195 + PflMI CCANNNNNTGG 191 188 . . 186 191 + BsiI* CACGAG 186 190 . . 190 193 + SetI ASST 193 189 . . 190 193 + CviJI RGCY 191 191 . . 190 193 + AluI AGCT 191 191 . . 195 200 + Hpy166II GTNNAC 197 197 . . 199 202 + SetI ASST 202 198 . . 200 203 + MnlI CCTC 210 209 . . 197 201 - HphI GGTGA 189 188 . . 210 213 + CviJI RGCY 211 211 . . 206 211 - Eco57MI CTGRAG 191 189 . . 206 211 - Eco57I CTGAAG 191 189 . . 226 230 + TauI GCSGC 229 226 . . 226 230 + BlsI GCNGC 228 227 . . 226 230 + BisI GCNGC 227 228 . . 228 231 + CviJI RGCY 229 229 . . 228 231 + HaeIII GGCC 229 229 . . 226 229 - AciI CCGC 226 228 . . 230 233 + MnlI CCTC 240 239 . . 233 236 + MnlI CCTC 243 242 . . 236 239 + MnlI CCTC 246 245 . . 231 236 - BseRI GAGGAG 222 220 . . 234 239 - BseRI GAGGAG 225 223 . . 243 248 + Hpy166II GTNNAC 245 245 . . 247 250 + SetI ASST 250 246 . . 248 251 + MnlI CCTC 258 257 . . 249 254 + Ksp632I* CTCTTC 255 258 . . 250 254 - MboII GAAGA 242 241 . . 255 258 + CviJI RGCY 256 256 . . 255 258 + HaeIII GGCC 256 256 . . 257 260 + MnlI CCTC 267 266 . . 259 264 + Hpy178III* TCNNGA 260 262 . . 262 267 + XhoII RGATCY 262 266 . . 262 266 + BinI* GGATC 270 271 . . 263 266 + DpnI GATC 264 264 . . 263 266 + BstKTI GATC 265 263 . . 263 266 + MboI GATC 262 266 . . 268 272 + MaeIII GTNAC 267 272 . . 277 281 + TfiI GAWTC 277 280 . . 277 281 + HinfI GANTC 277 280 . . 282 287 + TaqII CACCCA 298 296 . . 286 289 + FatI CATG 285 289 . . 286 289 + NlaIII CATG 289 285 . . 286 289 + CviAII CATG 286 288 . . 287 291 + TspDTI ATGAA 302 300 . . 291 294 + TspEI AATT 290 294 . . 295 298 + SetI ASST 298 294 . . 296 299 + MnlI CCTC 306 305 . . 301 304 + SetI ASST 304 300 . . 302 305 + MnlI CCTC 312 311 . . 304 307 + TaqI TCGA 304 306 . . 306 317 + DrdI GACNNNNNNGTC 312 310 . . 312 317 + BtrI CACGTC 314 314 . . 313 316 + TaiI ACGT 316 312 . . 313 316 + SetI ASST 316 312 . . 313 316 + MaeII ACGT 313 315 . . 315 320 + Hpy166II GTNNAC 317 317 . . 324 328 + BlsI GCNGC 326 325 . . 324 328 + BisI GCNGC 325 326 . . 324 328 + TseI GCWGC 324 327 . . 324 328 - BbvI GCAGC 311 315 . . 333 337 + Hin4II* CCTTC 343 342 . . 336 341 + Hpy178III* TCNNGA 337 339 . . 336 341 + BspHI TCATGA 336 340 . . 337 340 + FatI CATG 336 340 . . 337 340 + NlaIII CATG 340 336 . . 337 340 + CviAII CATG 337 339 . . 335 339 - TspDTI ATGAA 325 323 . . 340 344 + HinfI GANTC 340 343 . . 340 344 - PleI GAGTC 334 335 . . 340 344 - MlyI GAGTC 334 334 . . 349 359 + BsaXI ACNNNNNCTCC 339 336 369 366 354 358 + BsmAI GTCTC 359 363 . . 358 362 + BccI CCATC 366 367 . . 368 373 + PstI CTGCAG 372 368 . . 368 373 + SfeI* CTRYAG 368 372 . . 369 372 + CviRI* TGCA 370 370 . . 370 374 + BlsI GCNGC 372 371 . . 370 374 + BisI GCNGC 371 372 . . 370 374 + BbvI GCAGC 382 386 . . 370 374 + TseI GCWGC 370 373 . . 381 387 + TspRI CASTGNN 387 378 . . 383 387 + Tsp45I GTSAC 382 387 . . 383 387 + MaeIII GTNAC 382 387 . . 388 392 + MboII GAAGA 400 399 . . 391 395 + TfiI GAWTC 391 394 . . 391 395 + HinfI GANTC 391 394 . . 408 411 + CviRI* TGCA 409 409 . . 406 410 - SfaNI GCATC 396 400 . . 413 416 + SetI ASST 416 412 . . 432 435 + CviJI RGCY 433 433 . . 452 456 - HgaI GACGC 441 446 . . 454 458 - BslFI GGGAC 439 443 . . 472 476 + Tsp45I GTSAC 471 476 . . 472 476 + MaeIII GTNAC 471 476 . . 474 480 + TspRI CASTGNN 480 471 . . 475 479 + Tsp4CI* ACNGT 477 476 . . 478 482 + Tsp45I GTSAC 477 482 . . 478 482 + MaeIII GTNAC 477 482 . . 479 483 - HphI GGTGA 471 470 . . 487 492 + SduI GDGCHC 491 487 . . 487 492 + HgiJII* GRGCYC 491 487 . . 488 491 + CviJI RGCY 489 489 . . 493 497 + MaeIII GTNAC 492 497 . . 501 504 + Csp6I GTAC 501 503 . . 501 504 + RsaI GTAC 502 502 . . 498 502 - BsrI ACTGG 498 496 . . 497 502 - BfiI ACTGGG 492 491 . . 503 506 + TaiI ACGT 506 502 . . 503 506 + SetI ASST 506 502 . . 503 506 + MaeII ACGT 503 505 . . 505 509 + MaeIII GTNAC 504 509 . . 508 512 + Tsp4CI* ACNGT 510 509 . . 511 515 + Tsp45I GTSAC 510 515 . . 511 515 + MaeIII GTNAC 510 515 . . 512 516 - HphI GGTGA 504 503 . . 536 539 + TaiI ACGT 539 535 . . 536 539 + SetI ASST 539 535 . . 536 539 + MaeII ACGT 536 538 . . 538 542 + MaeIII GTNAC 537 542 . . 541 545 + Tsp4CI* ACNGT 543 542 . . 544 548 + Tsp45I GTSAC 543 548 . . 544 548 + MaeIII GTNAC 543 548 . . 546 553 + SgrAI CRCCGGYG 547 551 . . 547 552 + Cfr10I RCCGGY 547 551 . . 547 552 + BetI* WCCGGW 547 551 . . 547 552 + AgeI ACCGGT 547 551 . . 548 551 + HpaII CCGG 548 550 . . 545 549 - HphI GGTGA 537 536 . . 551 556 + Hpy166II GTNNAC 553 553 . . 551 556 + SduI GDGCHC 555 551 . . 551 556 + BseSI GKGCMC 555 551 . . 551 556 + ApaLI GTGCAC 551 555 . . 551 556 + HgiAI* GWGCWC 555 551 . . 552 555 + CviRI* TGCA 553 553 . . 555 558 + SetI ASST 558 554 . . 556 560 + Hin4II* CCTTC 566 565 . . 562 566 + MaeIII GTNAC 561 566 . . 567 578 + TstI CACNNNNNNTCC 558 553 590 585 568 578 + BsaXI ACNNNNNCTCC 558 555 588 585 571 574 + SetI ASST 574 570 . . 572 575 + MnlI CCTC 582 581 . . 577 581 + BssKI CCNGG 576 581 . . 577 581 + BseBI CCWGG 578 579 . . 577 581 + ScrFI CCNGG 578 579 . . 577 581 + EcoRII CCWGG 576 581 . . 579 582 + SetI ASST 582 578 . . 575 580 - Eco57MI CTGRAG 560 558 . . 575 580 - GsuI CTGGAG 560 558 . . 581 585 + MaeIII GTNAC 580 585 . . 584 587 + TaiI ACGT 587 583 . . 584 587 + SetI ASST 587 583 . . 584 587 + MaeII ACGT 584 586 . . 586 589 + Csp6I GTAC 586 588 . . 586 589 + RsaI GTAC 587 587 . . 594 597 + Csp6I GTAC 594 596 . . 594 597 + RsaI GTAC 595 595 . . 607 610 + TaqI TCGA 607 609 . . 616 619 + TspEI AATT 615 619 . . 619 622 + TaqI TCGA 619 621 . . 623 627 - BsrI ACTGG 623 621 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 179 454 # # AciI: # [CCGC] # 226 407 # # AgeI: # [ACCGGT] # 86 547 # # AluI: # [AGCT] # 87 104 442 # # ApaLI: # [GTGCAC] # 82 551 # # BbvI: # [GCAGC] # 71 251 311 # # BccI: # [CCATC] # 267 366 # # BetI*: # [WCCGGW] # 86 547 # # BfiI: # [ACTGGG] # 141 492 # # BinI*: # [GGATC] # 270 363 # # BisI: # [GCNGC] # 46 98 227 262 # # BlsI: # [GCNGC] # 46 98 228 261 # # BsaXI: # [ACNNNNNCTCC] # 30 30 45 189 339 # # BseBI: # [CCWGG] # 55 578 # # BseRI: # [GAGGAG] # 3 222 408 # # BseSI: # [GKGCMC] # 78 119 436 # # BseYI: # [CCCAGC] # 100 533 # # BsiI*: # [CACGAG] # 186 447 # # BsiYI*: # [CCNNNNNNNGG] # 191 442 # # BslFI: # [GGGAC] # 194 439 # # BsmAI: # [GTCTC] # 118 241 274 # # BspHI: # [TCATGA] # 297 336 # # BsrI: # [ACTGG] # 10 125 498 # # BssKI: # [CCNGG] # 57 576 # # BstKTI: # [GATC] # 265 368 # # BtrI: # [CACGTC] # 314 319 # # Cfr10I: # [RCCGGY] # 86 547 # # Csp6I: # [GTAC] # 8 39 85 138 363 # # CviAII: # [CATG] # 51 286 296 # # CviJI: # [RGCY] # 18 20 27 56 87 104 # 144 177 # # CviRI*: # [TGCA] # 39 80 144 370 # # DpnI: # [GATC] # 264 369 # # DrdI: # [GACNNNNNNGTC] # 312 321 # # Eco57I: # [CTGAAG] # 191 442 # # Eco57MI: # [CTGRAG] # 73 191 369 # # EcoRII: # [CCWGG] # 57 576 # # FatI: # [CATG] # 51 285 297 # # GlaI: # [GCGC] # 98 535 # # GsaI: # [CCCAGC] # 104 529 # # GsuI: # [CTGGAG] # 73 560 # # HaeII: # [RGCGCY] # 100 533 # # HaeIII: # [GGCC] # 27 229 377 # # HgaI: # [GACGC] # 192 441 # # HgiAI*: # [GWGCWC] # 78 555 # # HgiCI*: # [GGYRCC] # 114 519 # # HgiJII*: # [GRGCYC] # 142 491 # # HhaI: # [GCGC] # 99 534 # # Hin4II*: # [CCTTC] # 45 67 223 298 # # Hin6I: # [GCGC] # 97 536 # # HinfI: # [GANTC] # 51 63 242 277 # # HpaII: # [CCGG] # 85 548 # # HphI: # [GGTGA] # 30 33 33 96 159 282 # # Hpy166II: # [GTNNAC] # 17 48 72 80 180 236 # # Hpy178III*: # [TCNNGA] # 77 260 296 # # Hpy99I: # [CGWCG] # 148 485 # # Ksp632I*: # [CTCTTC] # 255 378 # # MaeII: # [ACGT] # 33 48 49 190 313 # # MaeIII: # [GTNAC] # 6 6 6 12 15 18 # 19 27 53 89 102 115 # 165 # # MboI: # [GATC] # 262 371 # # MboII: # [GAAGA] # 158 233 242 # # MlyI: # [GAGTC] # 299 334 # # MnlI: # [CCTC] # 3 3 6 9 12 30 # 30 39 51 180 270 # # MseI: # [TTAA] # 31 602 # # NlaIII: # [CATG] # 51 289 293 # # NlaIV: # [GGNNCC] # 116 517 # # PflMI: # [CCANNNNNTGG] # 191 442 # # PleI: # [GAGTC] # 299 334 # # PstI: # [CTGCAG] # 261 372 # # RsaI: # [GTAC] # 8 38 85 139 363 # # ScrFI: # [CCNGG] # 55 578 # # SduI: # [GDGCHC] # 64 78 119 372 # # SecI*: # [CCNNGG] # 110 523 # # SetI: # [ASST] # 5 6 8 9 12 12 # 16 19 21 33 37 46 # 48 48 54 69 90 100 # # SfaNI: # [GCATC] # 237 396 # # SfeI*: # [CTRYAG] # 265 368 # # SgrAI: # [CRCCGGYG] # 86 547 # # SplI*: # [CGTACG] # 137 496 # # StyI: # [CCWWGG] # 110 523 # # TaiI: # [ACGT] # 33 46 48 190 316 # # TaqI: # [TCGA] # 12 14 303 304 # # TaqII: # [CACCCA] # 298 335 # # TauI: # [GCSGC] # 229 404 # # TfiI: # [GAWTC] # 114 242 277 # # TseI: # [GCWGC] # 46 263 324 # # Tsp45I: # [GTSAC] # 6 33 33 89 90 165 # 217 # # Tsp4CI*: # [ACNGT] # 33 33 75 90 102 300 # # TspDTI: # [ATGAA] # 23 302 308 # # TspEI: # [AATT] # 18 290 325 # # TspRI: # [CASTGNN] # 93 153 387 # # TstI: # [CACNNNNNNTCC] # 32 43 558 # # XcmI: # [CCANNNNNNNNNTGG] # 108 525 # # XhoII: # [RGATCY] # 262 371 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 633 # Reported_sequences: 1 # Reported_hitcount: 196 #---------------------------------------