Restriction Fragments for DGA1/YOR245C



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The currently selected gene/sequence is :

DGA1/YOR245C on chromosome XV from coordinates 795332 to 794076.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.14109.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 4 9 + Hpy178III* TCNNGA 5 7 . . 26 30 + MboII GAAGA 38 37 . . 33 37 + MboII GAAGA 45 44 . . 29 33 - Hin4II* CCTTC 23 22 . . 40 44 + MboII GAAGA 52 51 . . 36 40 - Hin4II* CCTTC 30 29 . . 43 47 - Hin4II* CCTTC 37 36 . . 49 52 + CviJI RGCY 50 50 . . 52 62 + BsiYI* CCNNNNNNNGG 58 55 . . 53 58 + SfeI* CTRYAG 53 57 . . 57 60 + CviJI RGCY 58 58 . . 58 63 + Cfr10I RCCGGY 58 62 . . 59 62 + HpaII CCGG 59 61 . . 76 79 + FatI CATG 75 79 . . 76 79 + NlaIII CATG 79 75 . . 76 79 + CviAII CATG 76 78 . . 79 89 - Hin4I GAYNNNNNVTC 102 97 70 65 98 102 + SfaNI GCATC 107 111 . . 100 105 + ClaI ATCGAT 101 103 . . 101 104 + TaqI TCGA 101 103 . . 112 123 + PpiI GAACNNNNNCTC 104 99 136 131 117 121 + HinfI GANTC 117 120 . . 117 121 - PleI GAGTC 111 112 . . 117 121 - MlyI GAGTC 111 111 . . 133 136 + MaeI CTAG 133 135 . . 136 140 + PleI GAGTC 144 145 . . 136 140 + MlyI GAGTC 145 145 . . 136 140 + HinfI GANTC 136 139 . . 140 143 + FatI CATG 139 143 . . 140 143 + NlaIII CATG 143 139 . . 140 143 + CviAII CATG 140 142 . . 172 175 + SetI ASST 175 171 . . 174 178 + MaeIII GTNAC 173 178 . . 170 174 - Hin4II* CCTTC 164 163 . . 186 189 + CviJI RGCY 187 187 . . 188 193 + SfeI* CTRYAG 188 192 . . 194 197 + FatI CATG 193 197 . . 194 197 + NlaIII CATG 197 193 . . 194 197 + CviAII CATG 194 196 . . 205 209 - MboII GAAGA 197 196 . . 207 211 - TspDTI ATGAA 197 195 . . 213 218 + TatI WGTACW 213 217 . . 214 217 + Csp6I GTAC 214 216 . . 214 217 + RsaI GTAC 215 215 . . 217 222 + Ksp632I* CTCTTC 223 226 . . 218 222 - MboII GAAGA 210 209 . . 232 235 + TaiI ACGT 235 231 . . 232 235 + SetI ASST 235 231 . . 232 235 + MaeII ACGT 232 234 . . 240 243 + CviRI* TGCA 241 241 . . 245 250 + Hpy178III* TCNNGA 246 248 . . 247 250 + TaqI TCGA 247 249 . . 253 264 + BstXI CCANNNNNNTGG 260 256 . . 257 263 + TspRI CASTGNN 263 254 . . 258 262 + Tsp4CI* ACNGT 260 259 . . 299 302 + TaqI TCGA 299 301 . . 304 307 + SetI ASST 307 303 . . 305 310 + Eco31I GGTCTC 311 315 . . 306 310 + BsmAI GTCTC 311 315 . . 310 320 + BsiYI* CCNNNNNNNGG 316 313 . . 312 315 + CviRI* TGCA 313 313 . . 316 320 + BsrI ACTGG 321 319 . . 332 337 + ClaI ATCGAT 333 335 . . 333 336 + TaqI TCGA 333 335 . . 341 346 + Ksp632I* CTCTTC 347 350 . . 345 348 + TaqI TCGA 345 347 . . 342 346 - MboII GAAGA 334 333 . . 354 358 - TspDTI ATGAA 344 342 . . 356 361 - BsrDI GCAATG 355 353 . . 404 407 + MseI TTAA 404 406 . . 409 413 + Tsp4CI* ACNGT 411 410 . . 415 418 + TspEI AATT 414 418 . . 417 422 + AhaIII* TTTAAA 419 419 . . 418 421 + MseI TTAA 418 420 . . 454 459 + Hpy166II GTNNAC 456 456 . . 454 459 + HpaI GTTAAC 456 456 . . 454 459 + HindII GTYRAC 456 456 . . 455 458 + MseI TTAA 455 457 . . 466 469 + TspEI AATT 465 469 . . 484 489 + BalI TGGCCA 486 486 . . 484 489 + CfrI YGGCCR 484 488 . . 485 488 + CviJI RGCY 486 486 . . 485 488 + HaeIII GGCC 486 486 . . 491 495 + DdeI CTNAG 491 494 . . 487 500 - AjuI GAANNNNNNNTTGG 512 507 480 475 502 507 + VspI ATTAAT 503 505 . . 503 506 + MseI TTAA 503 505 . . 540 544 + BssKI CCNGG 539 544 . . 540 544 + BseBI CCWGG 541 542 . . 540 544 + ScrFI CCNGG 541 542 . . 540 544 + EcoRII CCWGG 539 544 . . 547 552 + TatI WGTACW 547 551 . . 547 552 + Bsp1407I TGTACA 547 551 . . 548 551 + Csp6I GTAC 548 550 . . 548 551 + RsaI GTAC 549 549 . . 553 557 + BmgT120I GGNCC 554 555 . . 553 557 + AsuI* GGNCC 553 556 . . 554 557 + CviJI RGCY 555 555 . . 554 557 + HaeIII GGCC 555 555 . . 559 562 + TaiI ACGT 562 558 . . 559 562 + SetI ASST 562 558 . . 559 562 + MaeII ACGT 559 561 . . 561 564 + Csp6I GTAC 561 563 . . 561 564 + RsaI GTAC 562 562 . . 571 577 + BstEII GGTNACC 571 576 . . 572 576 + MaeIII GTNAC 571 576 . . 570 575 - TsoI TARCCA 560 558 . . 576 580 + BccI CCATC 584 585 . . 577 581 - FokI GGATG 563 567 . . 577 581 - BseGI GGATG 576 574 . . 584 588 + BceAI ACGGC 600 602 . . 593 598 + SduI GDGCHC 597 593 . . 593 598 + HgiAI* GWGCWC 597 593 . . 595 605 + MwoI GCNNNNNNNGC 601 598 . . 595 605 + BstAPI GCANNNNNTGC 601 598 . . 596 604 + DraIII CACNNNGTG 601 598 . . 604 614 + MwoI GCNNNNNNNGC 610 607 . . 618 621 + CviRI* TGCA 619 619 . . 627 630 + SetI ASST 630 626 . . 625 629 - Hin4II* CCTTC 619 618 . . 632 636 + MaeIII GTNAC 631 636 . . 651 656 + SecI* CCNNGG 651 655 . . 652 656 + BssKI CCNGG 651 656 . . 652 656 + BseBI CCWGG 653 654 . . 652 656 + ScrFI CCNGG 653 654 . . 652 656 + EcoRII CCWGG 651 656 . . 654 657 + SetI ASST 657 653 . . 669 673 + Hpy188I TCNGA 671 670 . . 674 685 + BdaI TGANNNNNNTCA 663 661 697 695 675 684 + PshAI GACNNNNGTC 679 679 . . 677 683 + TspRI CASTGNN 683 674 . . 678 682 + BsrI ACTGG 683 681 . . 682 686 + Tsp45I GTSAC 681 686 . . 682 686 + MaeIII GTNAC 681 686 . . 674 685 - BdaI TGANNNNNNTCA 697 695 663 661 689 692 + TspEI AATT 688 692 . . 692 696 - TspDTI ATGAA 682 680 . . 710 714 - BsmAI GTCTC 704 708 . . 729 732 + SetI ASST 732 728 . . 736 740 - MboII GAAGA 728 727 . . 737 742 - Eco57MI CTGRAG 722 720 . . 737 742 - Eco57I CTGAAG 722 720 . . 747 751 + Hpy188I TCNGA 749 748 . . 760 763 + MseI TTAA 760 762 . . 765 769 + HinfI GANTC 765 768 . . 765 769 - PleI GAGTC 759 760 . . 765 769 - MlyI GAGTC 759 759 . . 769 773 + DdeI CTNAG 769 772 . . 784 787 + TaqI TCGA 784 786 . . 786 789 + DpnI GATC 787 787 . . 786 789 + BstKTI GATC 788 786 . . 786 789 + MboI GATC 785 789 . . 790 793 + CviRI* TGCA 791 791 . . 805 810 + HaeII RGCGCY 809 805 . . 806 809 + Hin6I GCGC 806 808 . . 806 809 + GlaI GCGC 807 807 . . 806 809 + HhaI GCGC 808 806 . . 809 812 + MaeI CTAG 809 811 . . 814 818 + TfiI GAWTC 814 817 . . 814 818 + HinfI GANTC 814 817 . . 823 826 + MseI TTAA 823 825 . . 839 844 + Hpy166II GTNNAC 841 841 . . 839 842 + Csp6I GTAC 839 841 . . 839 842 + RsaI GTAC 840 840 . . 852 857 + AhaIII* TTTAAA 854 854 . . 853 856 + MseI TTAA 853 855 . . 875 878 + MseI TTAA 875 877 . . 879 883 + BsrI ACTGG 884 882 . . 881 886 + BalI TGGCCA 883 883 . . 881 886 + CfrI YGGCCR 881 885 . . 882 885 + CviJI RGCY 883 883 . . 882 885 + HaeIII GGCC 883 883 . . 898 903 + SspI AATATT 900 900 . . 902 905 + MseI TTAA 902 904 . . 905 908 + SetI ASST 908 904 . . 907 910 + MaeI CTAG 907 909 . . 921 924 + CviRI* TGCA 922 922 . . 932 935 + SetI ASST 935 931 . . 931 934 - MnlI CCTC 924 923 . . 934 939 + Hpy166II GTNNAC 936 936 . . 938 942 + Tsp4CI* ACNGT 940 939 . . 942 947 + PsiI TTATAA 944 944 . . 951 955 + Hpy188I TCNGA 953 952 . . 952 956 + DdeI CTNAG 952 955 . . 954 959 + SduI GDGCHC 958 954 . . 954 959 + HgiAI* GWGCWC 958 954 . . 952 956 - BspCNI CTCAG 944 942 . . 952 956 - BseMII CTCAG 943 941 . . 967 971 + TfiI GAWTC 967 970 . . 967 971 + HinfI GANTC 967 970 . . 975 979 + BssKI CCNGG 974 979 . . 975 979 + BseBI CCWGG 976 977 . . 975 980 + SecI* CCNNGG 975 979 . . 975 979 + ScrFI CCNGG 976 977 . . 975 979 + EcoRII CCWGG 974 979 . . 986 989 + CviRI* TGCA 987 987 . . 1040 1043 - MnlI CCTC 1033 1032 . . 1051 1054 + TspEI AATT 1050 1054 . . 1080 1085 + HaeII RGCGCY 1084 1080 . . 1081 1084 + Hin6I GCGC 1081 1083 . . 1081 1084 + GlaI GCGC 1082 1082 . . 1081 1084 + HhaI GCGC 1083 1081 . . 1079 1089 - Hin4I GAYNNNNNVTC 1102 1097 1070 1065 1099 1104 - MmeI TCCRAC 1080 1078 . . 1105 1110 + StuI AGGCCT 1107 1107 . . 1106 1109 + CviJI RGCY 1107 1107 . . 1106 1109 + HaeIII GGCC 1107 1107 . . 1103 1107 - Hin4II* CCTTC 1097 1096 . . 1115 1118 + TaiI ACGT 1118 1114 . . 1115 1118 + SetI ASST 1118 1114 . . 1115 1118 + MaeII ACGT 1115 1117 . . 1138 1141 + AciI CCGC 1138 1140 . . 1137 1142 - EciI GGCGGA 1127 1125 . . 1154 1157 + MseI TTAA 1154 1156 . . 1159 1168 + MslI CAYNNNNRTG 1163 1163 . . 1165 1168 + FatI CATG 1164 1168 . . 1165 1168 + NlaIII CATG 1168 1164 . . 1165 1168 + CviAII CATG 1165 1167 . . 1180 1183 - AciI CCGC 1180 1182 . . 1224 1229 + KpnI GGTACC 1228 1224 . . 1224 1229 + NlaIV GGNNCC 1226 1226 . . 1224 1229 + HgiCI* GGYRCC 1224 1228 . . 1224 1229 + Acc65I GGTACC 1224 1228 . . 1225 1228 + Csp6I GTAC 1225 1227 . . 1225 1228 + RsaI GTAC 1226 1226 . . 1227 1232 + BetI* WCCGGW 1227 1231 . . 1228 1231 + HpaII CCGG 1228 1230 . . 1230 1234 + FokI GGATG 1243 1247 . . 1230 1234 + BseGI GGATG 1236 1234 . . 1233 1236 + CviRI* TGCA 1234 1234 . . 1231 1235 - SfaNI GCATC 1221 1225 . . 1238 1241 + TspEI AATT 1237 1241 . . 1242 1246 + MboII GAAGA 1254 1253 . . #--------------------------------------- # # # Acc65I: # [GGTACC] # 33 1224 # # AciI: # [CCGC] # 42 77 1138 # # AhaIII*: # [TTTAAA] # 403 419 435 # # AjuI: # [GAANNNNNNNTTGG] # 32 480 745 # # AsuI*: # [GGNCC] # 553 704 # # BalI: # [TGGCCA] # 374 397 486 # # BccI: # [CCATC] # 584 673 # # BceAI: # [ACGGC] # 600 657 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 560 663 # # BetI*: # [WCCGGW] # 30 1227 # # BmgT120I: # [GGNCC] # 554 703 # # BseBI: # [CCWGG] # 112 281 323 541 # # BseGI: # [GGATG] # 21 576 660 # # BseMII: # [CTCAG] # 314 943 # # BsiYI*: # [CCNNNNNNNGG] # 58 258 941 # # BsmAI: # [GTCTC] # 311 393 553 # # Bsp1407I: # [TGTACA] # 547 710 # # BspCNI: # [CTCAG] # 313 944 # # BsrDI: # [GCAATG] # 355 902 # # BsrI: # [ACTGG] # 201 321 362 373 # # BssKI: # [CCNGG] # 112 283 323 539 # # BstAPI: # [GCANNNNNTGC] # 601 656 # # BstEII: # [GGTNACC] # 571 686 # # BstKTI: # [GATC] # 469 788 # # BstXI: # [CCANNNNNNTGG] # 260 997 # # Cfr10I: # [RCCGGY] # 58 1199 # # CfrI: # [YGGCCR] # 376 397 484 # # ClaI: # [ATCGAT] # 101 232 924 # # Csp6I: # [GTAC] # 13 32 214 278 334 386 # # CviAII: # [CATG] # 54 64 76 92 971 # # CviJI: # [RGCY] # 8 50 69 129 150 224 # 299 328 # # CviRI*: # [TGCA] # 23 65 72 131 172 241 # 247 306 # # DdeI: # [CTNAG] # 183 278 305 491 # # DpnI: # [GATC] # 470 787 # # DraIII: # [CACNNNGTG] # 601 656 # # EciI: # [GGCGGA] # 130 1127 # # Eco31I: # [GGTCTC] # 311 946 # # Eco57I: # [CTGAAG] # 535 722 # # Eco57MI: # [CTGRAG] # 535 722 # # EcoRII: # [CCWGG] # 112 283 323 539 # # FatI: # [CATG] # 54 64 75 93 971 # # FokI: # [GGATG] # 14 563 680 # # GlaI: # [GCGC] # 175 275 807 # # HaeII: # [RGCGCY] # 173 275 809 # # HaeIII: # [GGCC] # 69 150 224 328 486 # # HgiAI*: # [GWGCWC] # 299 361 597 # # HgiCI*: # [GGYRCC] # 33 1224 # # HhaI: # [GCGC] # 174 275 808 # # Hin4I: # [GAYNNNNNVTC] # 32 32 70 155 968 # # Hin4II*: # [CCTTC] # 7 7 23 127 160 455 # 478 # # Hin6I: # [GCGC] # 176 275 806 # # HindII: # [GTYRAC] # 456 801 # # HinfI: # [GANTC] # 19 49 117 153 290 629 # # HpaI: # [GTTAAC] # 456 801 # # HpaII: # [CCGG] # 29 59 1169 # # Hpy166II: # [GTNNAC] # 95 321 385 456 # # Hpy178III*: # [TCNNGA] # 5 241 1011 # # Hpy188I: # [TCNGA] # 78 204 304 671 # # KpnI: # [GGTACC] # 29 1228 # # Ksp632I*: # [CTCTTC] # 124 223 910 # # MaeI: # [CTAG] # 98 133 350 676 # # MaeII: # [ACGT] # 142 232 327 556 # # MaeIII: # [GTNAC] # 50 60 173 398 576 # # MboI: # [GATC] # 472 785 # # MboII: # [GAAGA] # 3 7 7 13 38 124 # 145 394 526 # # MlyI: # [GAGTC] # 34 111 498 614 # # MmeI: # [TCCRAC] # 177 1080 # # MnlI: # [CCTC] # 109 224 924 # # MseI: # [TTAA] # 14 22 27 30 37 48 # 63 103 252 257 404 # # MslI: # [CAYNNNNRTG] # 94 1163 # # MwoI: # [GCNNNNNNNGC] # 9 601 647 # # NlaIII: # [CATG] # 54 64 79 89 971 # # NlaIV: # [GGNNCC] # 31 1226 # # PleI: # [GAGTC] # 33 111 498 615 # # PpiI: # [GAACNNNNNCTC] # 32 104 1121 # # PshAI: # [GACNNNNGTC] # 578 679 # # PsiI: # [TTATAA] # 313 944 # # RsaI: # [GTAC] # 13 31 215 278 334 386 # # ScrFI: # [CCNGG] # 112 281 323 541 # # SduI: # [GDGCHC] # 299 361 597 # # SecI*: # [CCNNGG] # 282 324 651 # # SetI: # [ASST] # 27 27 60 68 72 75 # 139 175 176 183 255 # # SfaNI: # [GCATC] # 36 107 1114 # # SfeI*: # [CTRYAG] # 53 135 1069 # # SspI: # [AATATT] # 357 900 # # StuI: # [AGGCCT] # 150 1107 # # TaiI: # [ACGT] # 139 235 327 556 # # TaqI: # [TCGA] # 12 34 52 101 146 439 # 473 # # TatI: # [WGTACW] # 213 334 710 # # TfiI: # [GAWTC] # 153 290 814 # # TsoI: # [TARCCA] # 560 697 # # Tsp45I: # [GTSAC] # 576 681 # # Tsp4CI*: # [ACNGT] # 151 260 317 529 # # TspDTI: # [ATGAA] # 147 197 338 575 # # TspEI: # [AATT] # 20 51 187 223 362 414 # # TspRI: # [CASTGNN] # 263 420 574 # # VspI: # [ATTAAT] # 503 754 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1257 # Reported_sequences: 1 # Reported_hitcount: 233 #---------------------------------------