Restriction Fragments for MGE1/YOR232W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

MGE1/YOR232W on chromosome XV from coordinates 774573 to 775259.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.28327.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 6 9 + SetI ASST 9 5 . . 6 9 + CviJI RGCY 7 7 . . 6 9 + AluI AGCT 7 7 . . 7 17 + MwoI GCNNNNNNNGC 13 10 . . 14 19 + EcoP15I CAGCAG 44 46 . . 16 20 + BlsI GCNGC 18 17 . . 16 20 + BisI GCNGC 17 18 . . 16 20 + BbvI GCAGC 28 32 . . 16 20 + TseI GCWGC 16 19 . . 18 21 + CviJI RGCY 19 19 . . 20 30 + BsiYI* CCNNNNNNNGG 26 23 . . 22 26 + Tsp4CI* ACNGT 24 23 . . 29 33 + BmgT120I GGNCC 30 31 . . 29 33 + AsuI* GGNCC 29 32 . . 30 33 + CviJI RGCY 31 31 . . 30 33 + HaeIII GGCC 31 31 . . 32 42 + BsiYI* CCNNNNNNNGG 38 35 . . 38 41 + MaeI CTAG 38 40 . . 42 51 + XmnI GAANNNNTTC 46 46 . . 51 60 + MslI CAYNNNNRTG 55 55 . . 49 53 - TspDTI ATGAA 39 37 . . 51 55 - FokI GGATG 37 41 . . 51 55 - BseGI GGATG 50 48 . . 60 65 + NlaIV GGNNCC 62 62 . . 60 65 + HgiCI* GGYRCC 60 64 . . 66 76 + FalI AAGNNNNNCTT 57 52 89 84 66 76 - FalI AAGNNNNNCTT 89 84 57 52 79 83 + Tsp45I GTSAC 78 83 . . 79 83 + MaeIII GTNAC 78 83 . . 81 85 + HinfI GANTC 81 84 . . 81 85 - PleI GAGTC 75 76 . . 81 85 - MlyI GAGTC 75 75 . . 85 89 + BccI CCATC 93 94 . . 106 109 + CviJI RGCY 107 107 . . 116 120 + MboII GAAGA 128 127 . . 133 137 + Hpy188I TCNGA 135 134 . . 137 141 + TspDTI ATGAA 152 150 . . 141 144 + CviJI RGCY 142 142 . . 151 155 + MboII GAAGA 163 162 . . 154 158 + TfiI GAWTC 154 157 . . 154 158 + HinfI GANTC 154 157 . . 170 174 + TspDTI ATGAA 185 183 . . 172 176 + MboII GAAGA 184 183 . . 182 187 + Eco57MI CTGRAG 203 201 . . 182 187 + Eco57I CTGAAG 203 201 . . 184 188 + MboII GAAGA 196 195 . . 184 189 - Ksp632I* CTCTTC 179 182 . . 184 190 - SapI GCTCTTC 179 182 . . 193 197 + Hpy188I TCNGA 195 194 . . 200 205 + Hpy178III* TCNNGA 201 203 . . 206 209 + TspEI AATT 205 209 . . 214 217 + CviJI RGCY 215 215 . . 215 225 + MwoI GCNNNNNNNGC 221 218 . . 220 223 + MseI TTAA 220 222 . . 216 220 - BsrI ACTGG 216 214 . . 224 227 + Hin6I GCGC 224 226 . . 224 227 + GlaI GCGC 225 225 . . 224 227 + HhaI GCGC 226 224 . . 225 228 + FnuDII* CGCG 226 226 . . 228 232 + MboII GAAGA 240 239 . . 228 233 + BbvII* GAAGAC 235 239 . . 239 244 + HindIII AAGCTT 239 243 . . 240 243 + SetI ASST 243 239 . . 240 243 + CviJI RGCY 241 241 . . 240 243 + AluI AGCT 241 241 . . 244 248 + Hpy188I TCNGA 246 245 . . 250 255 + SmlI CTYRAG 250 254 . . 250 255 - Bce83I* CTTGAG 235 233 . . 265 268 + MseI TTAA 265 267 . . 268 273 + XhoII RGATCY 268 272 . . 268 273 + BglII AGATCT 268 272 . . 269 272 + DpnI GATC 270 270 . . 269 272 + BstKTI GATC 271 269 . . 269 272 + MboI GATC 268 272 . . 284 288 + Hpy188I TCNGA 286 285 . . 288 293 + ApoI RAATTY 288 292 . . 289 292 + TspEI AATT 288 292 . . 301 305 + Tsp45I GTSAC 300 305 . . 301 305 + MaeIII GTNAC 300 305 . . 309 313 - Hin4II* CCTTC 303 302 . . 318 322 + Hpy188I TCNGA 320 319 . . 324 327 + SetI ASST 327 323 . . 324 327 + CviJI RGCY 325 325 . . 324 327 + AluI AGCT 325 325 . . 326 330 + DdeI CTNAG 326 329 . . 325 331 - Bpu10I CCTNAGC 326 329 . . 351 354 + CviRI* TGCA 352 352 . . 367 371 + TfiI GAWTC 367 370 . . 367 371 + HinfI GANTC 367 370 . . 371 376 + SfeI* CTRYAG 371 375 . . 388 391 + FatI CATG 387 391 . . 388 391 + NlaIII CATG 391 387 . . 388 391 + CviAII CATG 388 390 . . 391 401 + MwoI GCNNNNNNNGC 397 394 . . 396 401 + BsmI GAATGC 402 400 . . 403 408 + AhaIII* TTTAAA 405 405 . . 404 407 + MseI TTAA 404 406 . . 409 412 - MnlI CCTC 402 401 . . 412 416 + MboII GAAGA 424 423 . . 412 417 + BbvII* GAAGAC 419 423 . . 428 433 + StyI CCWWGG 428 432 . . 428 433 + SecI* CCNNGG 428 432 . . 435 438 + TspEI AATT 434 438 . . 447 452 + Hpy166II GTNNAC 449 449 . . 447 452 + BssNAI GTATAC 449 449 . . 447 452 + AccI GTMKAC 448 450 . . 490 494 + DdeI CTNAG 490 493 . . 500 504 + Tsp4CI* ACNGT 502 501 . . 512 515 + TspEI AATT 511 515 . . 538 541 + DpnI GATC 539 539 . . 538 541 + BstKTI GATC 540 538 . . 538 541 + MboI GATC 537 541 . . 538 542 - BinI* GGATC 532 533 . . 558 563 + AclI AACGTT 559 561 . . 559 562 + TaiI ACGT 562 558 . . 559 562 + SetI ASST 562 558 . . 559 562 + MaeII ACGT 559 561 . . 563 566 + TaqI TCGA 563 565 . . 576 579 + SetI ASST 579 575 . . 585 590 + NlaIV GGNNCC 587 587 . . 589 593 + BssKI CCNGG 588 593 . . 589 592 + HpaII CCGG 589 591 . . 589 593 + ScrFI CCNGG 590 591 . . 589 593 + CauII* CCSGG 590 591 . . 593 596 + Csp6I GTAC 593 595 . . 593 596 + RsaI GTAC 594 594 . . 595 599 + Tsp4CI* ACNGT 597 596 . . 604 607 + FatI CATG 603 607 . . 604 607 + NlaIII CATG 607 603 . . 604 607 + CviAII CATG 604 606 . . 606 611 + TatI WGTACW 606 610 . . 606 611 + Bsp1407I TGTACA 606 610 . . 607 610 + Csp6I GTAC 607 609 . . 607 610 + RsaI GTAC 608 608 . . 614 617 + TspEI AATT 613 617 . . 618 621 + SetI ASST 621 617 . . 625 628 + SetI ASST 628 624 . . 623 627 - HphI GGTGA 615 614 . . 644 648 + Hpy188I TCNGA 646 645 . . 659 663 + Hpy188I TCNGA 661 660 . . 663 666 + TspEI AATT 662 666 . . 658 663 - MmeI TCCRAC 639 637 . . 668 671 + MseI TTAA 668 670 . . 676 681 - Ksp632I* CTCTTC 671 674 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 239 448 # # AclI: # [AACGTT] # 128 559 # # AhaIII*: # [TTTAAA] # 282 405 # # AluI: # [AGCT] # 7 84 234 362 # # ApoI: # [RAATTY] # 288 399 # # AsuI*: # [GGNCC] # 29 658 # # BbvI: # [GCAGC] # 28 659 # # BbvII*: # [GAAGAC] # 184 235 268 # # BccI: # [CCATC] # 93 594 # # Bce83I*: # [CTTGAG] # 235 452 # # BglII: # [AGATCT] # 268 419 # # BinI*: # [GGATC] # 155 532 # # BisI: # [GCNGC] # 17 670 # # BlsI: # [GCNGC] # 18 669 # # BmgT120I: # [GGNCC] # 30 657 # # Bpu10I: # [CCTNAGC] # 326 361 # # BseGI: # [GGATG] # 50 637 # # BsiYI*: # [CCNNNNNNNGG] # 12 26 649 # # BsmI: # [GAATGC] # 285 402 # # Bsp1407I: # [TGTACA] # 81 606 # # BsrI: # [ACTGG] # 216 471 # # BssKI: # [CCNGG] # 99 588 # # BssNAI: # [GTATAC] # 238 449 # # BstKTI: # [GATC] # 147 269 271 # # CauII*: # [CCSGG] # 97 590 # # Csp6I: # [GTAC] # 14 80 593 # # CviAII: # [CATG] # 83 216 388 # # CviJI: # [RGCY] # 7 12 12 26 35 73 # 76 84 362 # # CviRI*: # [TGCA] # 335 352 # # DdeI: # [CTNAG] # 164 197 326 # # DpnI: # [GATC] # 148 269 270 # # Eco57I: # [CTGAAG] # 203 484 # # Eco57MI: # [CTGRAG] # 203 484 # # EcoP15I: # [CAGCAG] # 44 643 # # FalI: # [AAGNNNNNCTT] # 0 0 32 57 598 # # FatI: # [CATG] # 84 216 387 # # FnuDII*: # [CGCG] # 226 461 # # FokI: # [GGATG] # 37 650 # # GlaI: # [GCGC] # 225 462 # # HaeIII: # [GGCC] # 31 656 # # HgiCI*: # [GGYRCC] # 60 627 # # HhaI: # [GCGC] # 226 461 # # Hin4II*: # [CCTTC] # 303 384 # # Hin6I: # [GCGC] # 224 463 # # HindIII: # [AAGCTT] # 239 448 # # HinfI: # [GANTC] # 73 81 213 320 # # HpaII: # [CCGG] # 98 589 # # HphI: # [GGTGA] # 72 615 # # Hpy166II: # [GTNNAC] # 238 449 # # Hpy178III*: # [TCNNGA] # 201 486 # # Hpy188I: # [TCNGA] # 15 26 34 40 51 60 # 135 326 # # Ksp632I*: # [CTCTTC] # 16 179 492 # # MaeI: # [CTAG] # 38 649 # # MaeII: # [ACGT] # 128 559 # # MaeIII: # [GTNAC] # 78 222 387 # # MboI: # [GATC] # 150 268 269 # # MboII: # [GAAGA] # 12 21 35 44 128 184 # 263 # # MlyI: # [GAGTC] # 75 612 # # MmeI: # [TCCRAC] # 48 639 # # MnlI: # [CCTC] # 285 402 # # MseI: # [TTAA] # 19 45 139 220 264 # # MslI: # [CAYNNNNRTG] # 55 632 # # MwoI: # [GCNNNNNNNGC] # 13 176 208 290 # # NlaIII: # [CATG] # 80 216 391 # # NlaIV: # [GGNNCC] # 62 100 525 # # PleI: # [GAGTC] # 75 612 # # RsaI: # [GTAC] # 14 79 594 # # SapI: # [GCTCTTC] # 179 508 # # ScrFI: # [CCNGG] # 97 590 # # SecI*: # [CCNNGG] # 259 428 # # SetI: # [ASST] # 7 9 17 42 59 84 # 234 235 # # SfeI*: # [CTRYAG] # 316 371 # # SmlI: # [CTYRAG] # 250 437 # # StyI: # [CCWWGG] # 259 428 # # TaiI: # [ACGT] # 125 562 # # TaqI: # [TCGA] # 124 563 # # TatI: # [WGTACW] # 81 606 # # TfiI: # [GAWTC] # 154 213 320 # # TseI: # [GCWGC] # 16 671 # # Tsp45I: # [GTSAC] # 78 222 387 # # Tsp4CI*: # [ACNGT] # 24 90 95 478 # # TspDTI: # [ATGAA] # 33 39 113 502 # # TspEI: # [AATT] # 25 49 77 83 102 146 # 205 # # XhoII: # [RGATCY] # 268 419 # # XmnI: # [GAANNNNTTC] # 46 641 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 687 # Reported_sequences: 1 # Reported_hitcount: 144 #---------------------------------------