Restriction Fragments for YOR231C-A



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The currently selected gene/sequence is :

YOR231C-A on chromosome XV from coordinates 773363 to 773163.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.15373.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 15 20 + AhaIII* TTTAAA 17 17 . . 16 19 + MseI TTAA 16 18 . . 24 29 + SspI AATATT 26 26 . . 33 36 + DpnI GATC 34 34 . . 33 36 + BstKTI GATC 35 33 . . 33 36 + MboI GATC 32 36 . . 33 37 - BinI* GGATC 27 28 . . 45 48 + TspEI AATT 44 48 . . 64 67 + CviJI RGCY 65 65 . . 68 73 + Cfr10I RCCGGY 68 72 . . 69 72 + HpaII CCGG 69 71 . . 71 74 + CviJI RGCY 72 72 . . 71 76 + NlaIV GGNNCC 73 73 . . 75 79 + Hin4II* CCTTC 85 84 . . 82 85 + MaeI CTAG 82 84 . . 85 90 + BsmI GAATGC 91 89 . . 96 100 + BsmAI GTCTC 101 105 . . 97 102 + Hpy178III* TCNNGA 98 100 . . 99 102 + TaqI TCGA 99 101 . . 101 104 + DpnI GATC 102 102 . . 101 104 + BstKTI GATC 103 101 . . 101 104 + MboI GATC 100 104 . . 101 105 - BinI* GGATC 95 96 . . 111 116 + AhaIII* TTTAAA 113 113 . . 112 115 + MseI TTAA 112 114 . . 114 119 + ApoI RAATTY 114 118 . . 115 118 + TspEI AATT 114 118 . . 119 122 + CviRI* TGCA 120 120 . . 127 132 + MmeI TCCRAC 152 150 . . 137 141 - TspDTI ATGAA 127 125 . . 143 148 + MfeI CAATTG 143 147 . . 139 143 - FokI GGATG 125 129 . . 139 143 - BseGI GGATG 138 136 . . 144 147 + TspEI AATT 143 147 . . 147 152 + BclI TGATCA 147 151 . . 148 151 + DpnI GATC 149 149 . . 148 151 + BstKTI GATC 150 148 . . 148 157 + BsaBI GATNNNNATC 152 152 . . 148 151 + MboI GATC 147 151 . . 160 165 + Hpy166II GTNNAC 162 162 . . 160 163 + Csp6I GTAC 160 162 . . 160 163 + RsaI GTAC 161 161 . . 163 169 + TspRI CASTGNN 169 160 . . 164 168 + Tsp4CI* ACNGT 166 165 . . 169 172 + MseI TTAA 169 171 . . 179 182 + Csp6I GTAC 179 181 . . 179 182 + RsaI GTAC 180 180 . . 183 187 + TfiI GAWTC 183 186 . . 183 187 + HinfI GANTC 183 186 . . 186 190 + Hpy188I TCNGA 188 187 . . #--------------------------------------- # # # AhaIII*: # [TTTAAA] # 17 88 96 # # ApoI: # [RAATTY] # 87 114 # # BclI: # [TGATCA] # 54 147 # # BinI*: # [GGATC] # 27 68 106 # # BsaBI: # [GATNNNNATC] # 49 152 # # BseGI: # [GGATG] # 63 138 # # BsmAI: # [GTCTC] # 100 101 # # BsmI: # [GAATGC] # 91 110 # # BstKTI: # [GATC] # 35 47 51 68 # # Cfr10I: # [RCCGGY] # 68 133 # # Csp6I: # [GTAC] # 19 22 160 # # CviJI: # [RGCY] # 7 65 129 # # CviRI*: # [TGCA] # 81 120 # # DpnI: # [GATC] # 34 47 52 68 # # FokI: # [GGATG] # 76 125 # # Hin4II*: # [CCTTC] # 85 116 # # HinfI: # [GANTC] # 18 183 # # HpaII: # [CCGG] # 69 132 # # Hpy166II: # [GTNNAC] # 39 162 # # Hpy178III*: # [TCNNGA] # 98 103 # # Hpy188I: # [TCNGA] # 13 188 # # MaeI: # [CTAG] # 82 119 # # MboI: # [GATC] # 32 47 54 68 # # MfeI: # [CAATTG] # 58 143 # # MmeI: # [TCCRAC] # 49 152 # # MseI: # [TTAA] # 16 32 57 96 # # NlaIV: # [GGNNCC] # 73 128 # # RsaI: # [GTAC] # 19 21 161 # # SspI: # [AATATT] # 26 175 # # TaqI: # [TCGA] # 99 102 # # TfiI: # [GAWTC] # 18 183 # # Tsp4CI*: # [ACNGT] # 35 166 # # TspDTI: # [ATGAA] # 74 127 # # TspEI: # [AATT] # 29 44 58 70 # # TspRI: # [CASTGNN] # 32 169 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 201 # Reported_sequences: 1 # Reported_hitcount: 50 #---------------------------------------