Restriction Fragments for IAH1/YOR126C



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The currently selected gene/sequence is :

IAH1/YOR126C on chromosome XV from coordinates 560677 to 559961.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.2000.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 31 35 + TfiI GAWTC 31 34 . . 31 35 + HinfI GANTC 31 34 . . 43 48 + ApoI RAATTY 43 47 . . 44 47 + TspEI AATT 43 47 . . 53 56 + MseI TTAA 53 55 . . 59 62 + MaeI CTAG 59 61 . . 62 65 + CviJI RGCY 63 63 . . 62 65 + HaeIII GGCC 63 63 . . 62 66 + BmgT120I GGNCC 63 64 . . 62 66 + AsuI* GGNCC 62 65 . . 70 74 + MboII GAAGA 82 81 . . 73 77 - BccI CCATC 67 68 . . 82 85 + DpnI GATC 83 83 . . 82 85 + BstKTI GATC 84 82 . . 82 85 + MboI GATC 81 85 . . 91 101 + MwoI GCNNNNNNNGC 97 94 . . 97 102 + NlaIV GGNNCC 99 99 . . 99 102 + CviJI RGCY 100 100 . . 100 104 + TauI GCSGC 103 100 . . 100 104 + BlsI GCNGC 102 101 . . 100 104 + BisI GCNGC 101 102 . . 101 104 + AciI CCGC 101 103 . . 109 114 + Hpy166II GTNNAC 111 111 . . 109 114 + HindII GTYRAC 111 111 . . 138 142 - MboII GAAGA 130 129 . . 146 149 - MnlI CCTC 139 138 . . 159 164 + Hpy166II GTNNAC 161 161 . . 159 162 + Csp6I GTAC 159 161 . . 159 162 + RsaI GTAC 160 160 . . 166 171 + XbaI TCTAGA 166 170 . . 166 171 + Hpy178III* TCNNGA 167 169 . . 167 170 + MaeI CTAG 167 169 . . 170 174 - BccI CCATC 164 165 . . 189 194 + Hpy178III* TCNNGA 190 192 . . 191 195 + DdeI CTNAG 191 194 . . 191 195 - BspCNI CTCAG 183 181 . . 191 195 - BseMII CTCAG 182 180 . . 198 203 + AhaIII* TTTAAA 200 200 . . 199 202 + MseI TTAA 199 201 . . 205 208 + FatI CATG 204 208 . . 205 208 + NlaIII CATG 208 204 . . 205 208 + CviAII CATG 205 207 . . 206 210 + TspDTI ATGAA 221 219 . . 208 212 + TfiI GAWTC 208 211 . . 208 212 + HinfI GANTC 208 211 . . 212 226 + XcmI CCANNNNNNNNNTGG 219 218 . . 214 219 + SspI AATATT 216 216 . . 222 225 + FatI CATG 221 225 . . 222 225 + NlaIII CATG 225 221 . . 222 225 + CviAII CATG 222 224 . . 224 229 + BalI TGGCCA 226 226 . . 224 229 + CfrI YGGCCR 224 228 . . 225 228 + CviJI RGCY 226 226 . . 225 228 + HaeIII GGCC 226 226 . . 227 241 + XcmI CCANNNNNNNNNTGG 234 233 . . 231 236 + SspI AATATT 233 233 . . 241 246 + NlaIV GGNNCC 243 243 . . 241 246 + HgiCI* GGYRCC 241 245 . . 239 244 - TaqII CACCCA 229 227 . . 244 254 + MwoI GCNNNNNNNGC 250 247 . . 250 260 + Hin4I GAYNNNNNVTC 241 236 273 268 251 256 + EcoT22I ATGCAT 255 251 . . 252 255 + CviRI* TGCA 253 253 . . 253 258 + NspI RCATGY 257 253 . . 253 263 + MwoI GCNNNNNNNGC 259 256 . . 253 258 + Cac8I GCNNGC 255 255 . . 253 258 + SphI GCATGC 257 253 . . 250 254 - SfaNI GCATC 240 244 . . 254 257 + FatI CATG 253 257 . . 254 257 + NlaIII CATG 257 253 . . 254 257 + CviAII CATG 254 256 . . 257 263 + EspI* GCTNAGC 258 261 . . 258 262 + DdeI CTNAG 258 261 . . 258 262 + BspCNI CTCAG 271 269 . . 258 262 + BseMII CTCAG 272 270 . . 260 265 + EcoP15I CAGCAG 290 292 . . 264 267 + SetI ASST 267 263 . . 264 270 + PpuMI RGGWCCY 265 268 . . 264 270 + DraII RGGNCCY 265 268 . . 265 270 + NlaIV GGNNCC 267 267 . . 265 269 + BmgT120I GGNCC 266 267 . . 265 269 + AvaII GGWCC 265 268 . . 265 269 + AsuI* GGNCC 265 268 . . 262 267 - BspMI ACCTGC 253 257 . . 266 270 - BslFI GGGAC 251 255 . . 277 281 - BslFI GGGAC 262 266 . . 282 285 + MnlI CCTC 292 291 . . 289 294 + ApoI RAATTY 289 293 . . 290 293 + TspEI AATT 289 293 . . 295 300 + ClaI ATCGAT 296 298 . . 296 299 + TaqI TCGA 296 298 . . 301 306 + SspI AATATT 303 303 . . 325 329 + TspDTI ATGAA 340 338 . . 341 345 - TspGWI ACGGA 331 329 . . 350 355 + PsiI TTATAA 352 352 . . 358 362 + BmgT120I GGNCC 359 360 . . 358 362 + AvaII GGWCC 358 361 . . 358 362 + AsuI* GGNCC 358 361 . . 361 365 + BssKI CCNGG 360 365 . . 361 364 + HpaII CCGG 361 363 . . 361 366 + SecI* CCNNGG 361 365 . . 361 365 + ScrFI CCNGG 362 363 . . 361 365 + CauII* CCSGG 362 363 . . 365 368 + CviJI RGCY 366 366 . . 367 370 + MaeI CTAG 367 369 . . 400 404 + Hpy188I TCNGA 402 401 . . 401 406 + Eco57MI CTGRAG 422 420 . . 401 406 + Eco57I CTGAAG 422 420 . . 403 407 + MboII GAAGA 415 414 . . 411 414 + SetI ASST 414 410 . . 411 414 + CviJI RGCY 412 412 . . 411 414 + AluI AGCT 412 412 . . 416 420 + Hpy188I TCNGA 418 417 . . 422 432 + BaeI ACNNNNGTAYC 411 406 444 439 418 423 - BciVI GTATCC 412 411 . . 428 431 + Csp6I GTAC 428 430 . . 428 431 + RsaI GTAC 429 429 . . 425 429 - TspGWI ACGGA 415 413 . . 419 429 - BaeI ACNNNNGTAYC 444 439 411 406 435 446 + BcgI CGANNNNNNTGC 424 422 458 456 454 458 + Hpy188I TCNGA 456 455 . . 457 461 - SfaNI GCATC 447 451 . . 461 467 + Bpu10I CCTNAGC 462 465 . . 462 466 + DdeI CTNAG 462 465 . . 466 476 + MwoI GCNNNNNNNGC 472 469 . . 472 475 + MaeI CTAG 472 474 . . 473 478 + TsoI TARCCA 489 487 . . 474 477 + CviJI RGCY 475 475 . . 481 484 - MnlI CCTC 474 473 . . 484 493 + XmnI GAANNNNTTC 488 488 . . 494 498 + Hin4II* CCTTC 504 503 . . 501 504 + CviJI RGCY 502 502 . . 528 531 + SetI ASST 531 527 . . 526 530 - Hin4II* CCTTC 520 519 . . 532 536 + HphI GGTGA 544 543 . . 535 539 - SfaNI GCATC 525 529 . . 543 553 + MwoI GCNNNNNNNGC 549 546 . . 543 553 + BstAPI GCANNNNNTGC 549 546 . . 559 563 - BccI CCATC 553 554 . . 566 569 + CviRI* TGCA 567 567 . . 565 570 - BsgI GTGCAG 550 548 . . 574 579 + Hpy178III* TCNNGA 575 577 . . 574 579 + BetI* WCCGGW 574 578 . . 574 579 + BspMII* TCCGGA 574 578 . . 575 578 + HpaII CCGG 575 577 . . 575 585 + BsiYI* CCNNNNNNNGG 581 578 . . 585 588 + Csp6I GTAC 585 587 . . 585 588 + RsaI GTAC 586 586 . . 590 595 + ApoI RAATTY 590 594 . . 591 594 + TspEI AATT 590 594 . . 597 602 + Hpy178III* TCNNGA 598 600 . . 597 602 + BspHI TCATGA 597 601 . . 598 601 + FatI CATG 597 601 . . 598 601 + NlaIII CATG 601 597 . . 598 601 + CviAII CATG 598 600 . . 596 600 - TspDTI ATGAA 586 584 . . 605 608 + TspEI AATT 604 608 . . 614 617 + SetI ASST 617 613 . . 612 616 - Hin4II* CCTTC 606 605 . . 622 626 - BsmAI GTCTC 616 620 . . 643 647 - FokI GGATG 629 633 . . 643 647 - BseGI GGATG 642 640 . . 653 658 + NspI RCATGY 657 653 . . 654 657 + FatI CATG 653 657 . . 654 657 + NlaIII CATG 657 653 . . 654 657 + CviAII CATG 654 656 . . 656 659 + CviRI* TGCA 657 657 . . 659 664 + TatI WGTACW 659 663 . . 660 663 + Csp6I GTAC 660 662 . . 660 663 + RsaI GTAC 661 661 . . 688 691 + MaeI CTAG 688 690 . . 691 701 + Hin4I GAYNNNNNVTC 682 677 714 709 697 702 + XhoII RGATCY 697 701 . . 697 701 + BinI* GGATC 705 706 . . 698 701 + DpnI GATC 699 699 . . 698 701 + BstKTI GATC 700 698 . . 698 701 + MboI GATC 697 701 . . 694 698 - BccI CCATC 688 689 . . 691 701 - Hin4I GAYNNNNNVTC 714 709 682 677 712 717 + Hpy178III* TCNNGA 713 715 . . #--------------------------------------- # # # AciI: # [CCGC] # 101 616 # # AhaIII*: # [TTTAAA] # 200 517 # # AluI: # [AGCT] # 305 412 # # ApoI: # [RAATTY] # 43 127 246 301 # # AsuI*: # [GGNCC] # 62 93 203 359 # # AvaII: # [GGWCC] # 93 265 359 # # BaeI: # [ACNNNNGTAYC] # 0 0 33 273 411 # # BalI: # [TGGCCA] # 226 491 # # BccI: # [CCATC] # 29 67 97 135 389 # # BcgI: # [CGANNNNNNTGC] # 34 259 424 # # BciVI: # [GTATCC] # 305 412 # # BetI*: # [WCCGGW] # 143 574 # # BinI*: # [GGATC] # 12 705 # # BisI: # [GCNGC] # 101 616 # # BlsI: # [GCNGC] # 102 615 # # BmgT120I: # [GGNCC] # 63 93 203 358 # # Bpu10I: # [CCTNAGC] # 255 462 # # BseGI: # [GGATG] # 75 642 # # BseMII: # [CTCAG] # 90 182 445 # # BsgI: # [GTGCAG] # 167 550 # # BsiYI*: # [CCNNNNNNNGG] # 136 581 # # BslFI: # [GGGAC] # 11 251 455 # # BsmAI: # [GTCTC] # 101 616 # # BspCNI: # [CTCAG] # 88 183 446 # # BspHI: # [TCATGA] # 120 597 # # BspMI: # [ACCTGC] # 253 464 # # BspMII*: # [TCCGGA] # 143 574 # # BssKI: # [CCNGG] # 357 360 # # BstAPI: # [GCANNNNNTGC] # 168 549 # # BstKTI: # [GATC] # 17 84 616 # # Cac8I: # [GCNNGC] # 255 462 # # CauII*: # [CCSGG] # 355 362 # # CfrI: # [YGGCCR] # 224 493 # # ClaI: # [ATCGAT] # 296 421 # # Csp6I: # [GTAC] # 57 75 157 159 269 # # CviAII: # [CATG] # 17 32 56 63 205 344 # # CviJI: # [RGCY] # 27 37 46 63 63 126 # 140 215 # # CviRI*: # [TGCA] # 60 90 253 314 # # DdeI: # [CTNAG] # 67 191 204 255 # # DpnI: # [GATC] # 18 83 616 # # DraII: # [RGGNCCY] # 265 452 # # Eco57I: # [CTGAAG] # 295 422 # # Eco57MI: # [CTGRAG] # 295 422 # # EcoP15I: # [CAGCAG] # 290 427 # # EcoT22I: # [ATGCAT] # 255 462 # # EspI*: # [GCTNAGC] # 258 459 # # FatI: # [CATG] # 17 32 56 64 204 344 # # FokI: # [GGATG] # 88 629 # # HaeIII: # [GGCC] # 63 163 491 # # HgiCI*: # [GGYRCC] # 241 476 # # Hin4I: # [GAYNNNNNVTC] # 0 0 3 32 32 241 # 409 # # Hin4II*: # [CCTTC] # 16 86 111 504 # # HindII: # [GTYRAC] # 111 606 # # HinfI: # [GANTC] # 31 177 509 # # HpaII: # [CCGG] # 142 214 361 # # HphI: # [GGTGA] # 173 544 # # Hpy166II: # [GTNNAC] # 50 111 556 # # Hpy178III*: # [TCNNGA] # 4 23 23 115 167 385 # # Hpy188I: # [TCNGA] # 16 38 261 402 # # MaeI: # [CTAG] # 29 59 105 108 200 216 # # MboI: # [GATC] # 20 81 616 # # MboII: # [GAAGA] # 48 82 285 302 # # MnlI: # [CCTC] # 139 153 182 243 # # MseI: # [TTAA] # 53 146 518 # # MwoI: # [GCNNNNNNNGC] # 9 77 97 153 168 213 # # NlaIII: # [CATG] # 17 32 56 60 208 344 # # NlaIV: # [GGNNCC] # 24 99 144 450 # # NspI: # [RCATGY] # 60 257 400 # # PpuMI: # [RGGWCCY] # 265 452 # # PsiI: # [TTATAA] # 352 365 # # RsaI: # [GTAC] # 56 75 157 160 269 # # ScrFI: # [CCNGG] # 355 362 # # SecI*: # [CCNNGG] # 356 361 # # SetI: # [ASST] # 86 100 117 147 267 # # SfaNI: # [GCATC] # 78 192 207 240 # # SphI: # [GCATGC] # 257 460 # # SspI: # [AATATT] # 17 70 216 414 # # TaqI: # [TCGA] # 296 421 # # TaqII: # [CACCCA] # 229 488 # # TatI: # [WGTACW] # 58 659 # # TauI: # [GCSGC] # 103 614 # # TfiI: # [GAWTC] # 31 177 509 # # TsoI: # [TARCCA] # 228 489 # # TspDTI: # [ATGAA] # 119 131 221 246 # # TspEI: # [AATT] # 14 43 113 246 301 # # TspGWI: # [ACGGA] # 84 302 331 # # XbaI: # [TCTAGA] # 166 551 # # XcmI: # [CCANNNNNNNNNTGG] # 15 219 483 # # XhoII: # [RGATCY] # 20 697 # # XmnI: # [GAANNNNTTC] # 229 488 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 717 # Reported_sequences: 1 # Reported_hitcount: 180 #---------------------------------------