Restriction Fragments for RPO31/YOR116C



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The currently selected gene/sequence is :

RPO31/YOR116C on chromosome XV from coordinates 544145 to 539763.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.15060.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 1 5 + TspDTI ATGAA 16 14 . . 26 36 + BsiYI* CCNNNNNNNGG 32 29 . . 38 41 + MseI TTAA 38 40 . . 44 47 + CviJI RGCY 45 45 . . 49 54 + ApoI RAATTY 49 53 . . 50 53 + TspEI AATT 49 53 . . 55 60 + HaeII RGCGCY 59 55 . . 55 60 + Eco47III AGCGCT 57 57 . . 56 59 + Hin6I GCGC 56 58 . . 56 59 + GlaI GCGC 57 57 . . 56 66 + MwoI GCNNNNNNNGC 62 59 . . 56 59 + HhaI GCGC 58 56 . . 58 68 + MwoI GCNNNNNNNGC 64 61 . . 61 64 + MseI TTAA 61 63 . . 64 69 + HaeII RGCGCY 68 64 . . 64 69 + Eco47III AGCGCT 66 66 . . 65 68 + Hin6I GCGC 65 67 . . 65 68 + GlaI GCGC 66 66 . . 65 68 + HhaI GCGC 67 65 . . 67 71 + BlsI GCNGC 69 68 . . 67 71 + BisI GCNGC 68 69 . . 67 71 + TseI GCWGC 67 70 . . 67 71 - BbvI GCAGC 54 58 . . 88 92 + Hpy188I TCNGA 90 89 . . 84 88 - BsrI ACTGG 84 82 . . 83 88 - BfiI ACTGGG 78 77 . . 89 94 + Eco57MI CTGRAG 110 108 . . 89 94 + Eco57I CTGAAG 110 108 . . 102 107 + Hpy166II GTNNAC 104 104 . . 102 107 + AccI GTMKAC 103 105 . . 111 116 + XhoII RGATCY 111 115 . . 111 116 + BglII AGATCT 111 115 . . 112 115 + DpnI GATC 113 113 . . 112 115 + BstKTI GATC 114 112 . . 112 115 + MboI GATC 111 115 . . 115 120 + Ksp632I* CTCTTC 121 124 . . 119 122 + TaqI TCGA 119 121 . . 116 120 - MboII GAAGA 108 107 . . 133 136 + DpnI GATC 134 134 . . 133 136 + BstKTI GATC 135 133 . . 133 136 + MboI GATC 132 136 . . 137 142 + SduI GDGCHC 141 137 . . 137 142 + HgiAI* GWGCWC 141 137 . . 139 149 + MwoI GCNNNNNNNGC 145 142 . . 142 148 + SauI* CCTNAGG 143 146 . . 143 147 + DdeI CTNAG 143 146 . . 148 158 + MwoI GCNNNNNNNGC 154 151 . . 152 156 + Tsp4CI* ACNGT 154 153 . . 165 175 + BsiYI* CCNNNNNNNGG 171 168 . . 166 176 + BsiYI* CCNNNNNNNGG 172 169 . . 171 175 - BccI CCATC 165 166 . . 173 178 - TaqII CACCCA 163 161 . . 178 182 + BsmAI GTCTC 183 187 . . 180 185 + Ksp632I* CTCTTC 186 189 . . 185 188 + MnlI CCTC 195 194 . . 181 185 - MboII GAAGA 173 172 . . 186 191 - Eco57MI CTGRAG 171 169 . . 186 191 - GsuI CTGGAG 171 169 . . 192 197 + Hpy178III* TCNNGA 193 195 . . 188 192 - BsrI ACTGG 188 186 . . 201 204 + CviRI* TGCA 202 202 . . 205 208 + SetI ASST 208 204 . . 209 213 + Tsp45I GTSAC 208 213 . . 209 213 + MaeIII GTNAC 208 213 . . 212 216 + BceAI ACGGC 228 230 . . 223 227 + SfaNI GCATC 232 236 . . 232 235 + FatI CATG 231 235 . . 232 235 + NlaIII CATG 235 231 . . 232 235 + CviAII CATG 232 234 . . 234 239 + BalI TGGCCA 236 236 . . 234 239 + CfrI YGGCCR 234 238 . . 235 238 + CviJI RGCY 236 236 . . 235 238 + HaeIII GGCC 236 236 . . 245 249 + Tsp45I GTSAC 244 249 . . 245 249 + MaeIII GTNAC 244 249 . . 250 253 + MseI TTAA 250 252 . . 254 257 + TspEI AATT 253 257 . . 258 261 + SetI ASST 261 257 . . 258 261 + CviJI RGCY 259 259 . . 258 261 + AluI AGCT 259 259 . . 282 285 + Csp6I GTAC 282 284 . . 282 285 + RsaI GTAC 283 283 . . 291 294 + CviJI RGCY 292 292 . . 308 311 + CviRI* TGCA 309 309 . . 312 315 + SetI ASST 315 311 . . 326 330 + Tsp4CI* ACNGT 328 327 . . 320 332 - PsrI GAACNNNNNNTAC 344 339 312 307 346 350 + Hpy188I TCNGA 348 347 . . 365 368 + TspEI AATT 364 368 . . 371 374 + CviRI* TGCA 372 372 . . 373 376 + FatI CATG 372 376 . . 373 376 + NlaIII CATG 376 372 . . 373 376 + CviAII CATG 373 375 . . 374 378 + TspDTI ATGAA 389 387 . . 382 386 + Hpy99I CGWCG 386 381 . . 372 383 - BcgI CGANNNNNNTGC 395 393 361 359 388 392 + BssKI CCNGG 387 392 . . 388 392 + BseBI CCWGG 389 390 . . 388 392 + ScrFI CCNGG 389 390 . . 388 392 + EcoRII CCWGG 387 392 . . 400 403 + TspEI AATT 399 403 . . 403 406 + MseI TTAA 403 405 . . 408 411 + TaiI ACGT 411 407 . . 408 411 + SetI ASST 411 407 . . 408 411 + MaeII ACGT 408 410 . . 423 427 + MboII GAAGA 435 434 . . 423 436 + AjuI GAANNNNNNNTTGG 415 410 447 442 426 430 + MboII GAAGA 438 437 . . 435 439 + BmgT120I GGNCC 436 437 . . 435 439 + AvaII GGWCC 435 438 . . 435 439 + AsuI* GGNCC 435 438 . . 457 460 + SetI ASST 460 456 . . 455 459 - Hin4II* CCTTC 449 448 . . 464 467 + CviRI* TGCA 465 465 . . 465 476 + AlfI GCANNNNNNTGC 454 452 488 486 466 475 + MslI CAYNNNNRTG 470 470 . . 473 478 + SduI GDGCHC 477 473 . . 473 478 + HgiAI* GWGCWC 477 473 . . 465 476 - AlfI GCANNNNNNTGC 488 486 454 452 479 482 + MseI TTAA 479 481 . . 488 493 + Hpy178III* TCNNGA 489 491 . . 498 501 + CviJI RGCY 499 499 . . 499 503 + TauI GCSGC 502 499 . . 499 503 + BlsI GCNGC 501 500 . . 499 503 + BisI GCNGC 500 501 . . 500 505 + NspBII* CMGCKG 502 502 . . 500 503 + AciI CCGC 500 502 . . 502 512 + MwoI GCNNNNNNNGC 508 505 . . 502 506 + BlsI GCNGC 504 503 . . 502 506 + BisI GCNGC 503 504 . . 502 506 + TseI GCWGC 502 505 . . 502 506 - BbvI GCAGC 489 493 . . 503 508 - EcoP15I CAGCAG 475 477 . . 511 521 + MwoI GCNNNNNNNGC 517 514 . . 519 522 + CviJI RGCY 520 520 . . 520 524 + TauI GCSGC 523 520 . . 520 524 + BlsI GCNGC 522 521 . . 520 524 + BisI GCNGC 521 522 . . 521 524 + AciI CCGC 521 523 . . 522 525 + FnuDII* CGCG 523 523 . . 528 532 + MboII GAAGA 540 539 . . 538 541 + FatI CATG 537 541 . . 538 541 + NlaIII CATG 541 537 . . 538 541 + CviAII CATG 538 540 . . 558 561 + SetI ASST 561 557 . . 569 572 + AciI CCGC 569 571 . . 569 574 + BsrBI CCGCTC 571 571 . . 573 578 + Hpy178III* TCNNGA 574 576 . . 589 594 - TaqII CACCCA 579 577 . . 603 607 - Hin4II* CCTTC 597 596 . . 624 629 + Hpy178III* TCNNGA 625 627 . . 631 634 + MaeI CTAG 631 633 . . 647 652 + AclI AACGTT 648 650 . . 648 651 + TaiI ACGT 651 647 . . 648 651 + SetI ASST 651 647 . . 648 651 + MaeII ACGT 648 650 . . 657 661 + FokI GGATG 670 674 . . 657 661 + BseGI GGATG 663 661 . . 661 670 + BsaBI GATNNNNATC 665 665 . . 662 669 + SwaI ATTTAAAT 665 665 . . 663 668 + AhaIII* TTTAAA 665 665 . . 664 667 + MseI TTAA 664 666 . . 671 677 + TspRI CASTGNN 677 668 . . 681 686 + AhaIII* TTTAAA 683 683 . . 682 685 + MseI TTAA 682 684 . . 699 702 + TspEI AATT 698 702 . . 701 704 + MseI TTAA 701 703 . . 707 712 + PstI CTGCAG 711 707 . . 707 712 + SfeI* CTRYAG 707 711 . . 708 711 + CviRI* TGCA 709 709 . . 719 722 + SetI ASST 722 718 . . 719 722 + CviJI RGCY 720 720 . . 719 722 + AluI AGCT 720 720 . . 720 724 + BlsI GCNGC 722 721 . . 720 724 + BisI GCNGC 721 722 . . 720 724 + TseI GCWGC 720 723 . . 720 724 - BbvI GCAGC 707 711 . . 735 738 + CviRI* TGCA 736 736 . . 733 737 - SfaNI GCATC 723 727 . . 739 743 + Tsp4CI* ACNGT 741 740 . . 746 749 + MnlI CCTC 756 755 . . 752 757 + Hpy166II GTNNAC 754 754 . . 752 757 + AccI GTMKAC 753 755 . . 760 764 - BsmAI GTCTC 754 758 . . 768 773 + NdeI CATATG 769 771 . . 784 788 + FauI CCCGC 792 794 . . 785 788 + AciI CCGC 785 787 . . 789 792 + SetI ASST 792 788 . . 790 793 + MnlI CCTC 800 799 . . 792 797 + BetI* WCCGGW 792 796 . . 793 796 + HpaII CCGG 793 795 . . 805 810 + StuI AGGCCT 807 807 . . 806 809 + CviJI RGCY 807 807 . . 806 809 + HaeIII GGCC 807 807 . . 808 812 + Hin4II* CCTTC 818 817 . . 800 812 - BarI GAAGNNNNNNTAC 824 819 792 787 811 815 - TspGWI ACGGA 801 799 . . 821 824 + CviRI* TGCA 822 822 . . 819 823 - SfaNI GCATC 809 813 . . 826 830 + HinfI GANTC 826 829 . . 826 830 - PleI GAGTC 820 821 . . 826 830 - MlyI GAGTC 820 820 . . 835 839 + SfaNI GCATC 844 848 . . 830 835 - Eco57MI CTGRAG 815 813 . . 830 835 - GsuI CTGGAG 815 813 . . 844 848 + MboII GAAGA 856 855 . . 856 860 + Tsp4CI* ACNGT 858 857 . . 862 867 + HindIII AAGCTT 862 866 . . 863 866 + SetI ASST 866 862 . . 863 866 + CviJI RGCY 864 864 . . 863 866 + AluI AGCT 864 864 . . 868 872 + TspGWI ACGGA 883 881 . . 882 890 + Tth111I GACNNNGTC 885 886 . . 892 895 + MseI TTAA 892 894 . . 899 909 + FalI AAGNNNNNCTT 890 885 922 917 900 903 + CviJI RGCY 901 901 . . 901 906 + Cfr10I RCCGGY 901 905 . . 902 905 + HpaII CCGG 902 904 . . 906 911 + Hpy178III* TCNNGA 907 909 . . 899 909 - FalI AAGNNNNNCTT 922 917 890 885 926 929 + MseI TTAA 926 928 . . 936 940 - BccI CCATC 930 931 . . 964 968 + Tsp4CI* ACNGT 966 965 . . 969 972 + CviRI* TGCA 970 970 . . 970 975 + BsrDI GCAATG 977 975 . . 974 979 + TatI WGTACW 974 978 . . 974 979 + Bsp1407I TGTACA 974 978 . . 975 978 + Csp6I GTAC 975 977 . . 975 978 + RsaI GTAC 976 976 . . 984 988 + DdeI CTNAG 984 987 . . 984 988 + BspCNI CTCAG 997 995 . . 984 988 + BseMII CTCAG 998 996 . . 985 989 + Hpy188I TCNGA 987 986 . . 988 992 + HinfI GANTC 988 991 . . 988 996 + Tth111I GACNNNGTC 991 992 . . 990 994 + DdeI CTNAG 990 993 . . 990 994 + BspCNI CTCAG 1003 1001 . . 990 994 + BseMII CTCAG 1004 1002 . . 988 992 - PleI GAGTC 982 983 . . 988 992 - MlyI GAGTC 982 982 . . 1003 1008 + BtgZI GCGATG 1018 1022 . . 1005 1009 - SfaNI GCATC 995 999 . . 1011 1017 + SexAI ACCWGGT 1011 1016 . . 1012 1016 + BssKI CCNGG 1011 1016 . . 1012 1016 + BseBI CCWGG 1013 1014 . . 1012 1016 + ScrFI CCNGG 1013 1014 . . 1012 1016 + EcoRII CCWGG 1011 1016 . . 1014 1017 + SetI ASST 1017 1013 . . 1017 1021 - TspDTI ATGAA 1007 1005 . . 1019 1023 - FokI GGATG 1005 1009 . . 1019 1023 - BseGI GGATG 1018 1016 . . 1028 1032 + TauI GCSGC 1031 1028 . . 1028 1032 + BlsI GCNGC 1030 1029 . . 1028 1032 + BisI GCNGC 1029 1030 . . 1030 1035 + EciI GGCGGA 1046 1044 . . 1028 1031 - AciI CCGC 1028 1030 . . 1031 1034 - AciI CCGC 1031 1033 . . 1045 1055 + BsiYI* CCNNNNNNNGG 1051 1048 . . 1047 1050 + TspEI AATT 1046 1050 . . 1052 1055 - MnlI CCTC 1045 1044 . . 1076 1081 + Hpy166II GTNNAC 1078 1078 . . 1083 1086 + SetI ASST 1086 1082 . . 1086 1089 + TaqI TCGA 1086 1088 . . 1088 1092 + TfiI GAWTC 1088 1091 . . 1088 1092 + HinfI GANTC 1088 1091 . . 1099 1102 + TspEI AATT 1098 1102 . . 1118 1121 + TaqI TCGA 1118 1120 . . 1135 1139 + Tsp4CI* ACNGT 1137 1136 . . 1146 1151 + Hpy178III* TCNNGA 1147 1149 . . 1146 1151 + BetI* WCCGGW 1146 1150 . . 1146 1151 + BspMII* TCCGGA 1146 1150 . . 1147 1150 + HpaII CCGG 1147 1149 . . 1149 1154 + NlaIV GGNNCC 1151 1151 . . 1149 1153 + BmgT120I GGNCC 1150 1151 . . 1149 1153 + AvaII GGWCC 1149 1152 . . 1149 1153 + AsuI* GGNCC 1149 1152 . . 1172 1175 + SetI ASST 1175 1171 . . 1171 1174 - MnlI CCTC 1164 1163 . . 1180 1184 - BslFI GGGAC 1165 1169 . . 1186 1189 + DpnI GATC 1187 1187 . . 1186 1189 + BstKTI GATC 1188 1186 . . 1186 1189 + MboI GATC 1185 1189 . . 1194 1197 + CviRI* TGCA 1195 1195 . . 1202 1207 + Hpy178III* TCNNGA 1203 1205 . . 1207 1210 + TaiI ACGT 1210 1206 . . 1207 1210 + SetI ASST 1210 1206 . . 1207 1210 + MaeII ACGT 1207 1209 . . 1209 1214 + BciVI GTATCC 1220 1219 . . 1212 1217 + Hpy178III* TCNNGA 1213 1215 . . 1220 1223 + SetI ASST 1223 1219 . . 1222 1226 + MaeIII GTNAC 1221 1226 . . 1228 1231 + SetI ASST 1231 1227 . . 1244 1247 + TspEI AATT 1243 1247 . . 1253 1256 + TspEI AATT 1252 1256 . . 1254 1259 + VspI ATTAAT 1255 1257 . . 1255 1258 + MseI TTAA 1255 1257 . . 1257 1260 + TspEI AATT 1256 1260 . . 1267 1272 + NlaIV GGNNCC 1269 1269 . . 1267 1271 + BmgT120I GGNCC 1268 1269 . . 1267 1271 + AvaII GGWCC 1267 1270 . . 1267 1271 + AsuI* GGNCC 1267 1270 . . 1268 1278 + Hin4I GAYNNNNNVTC 1259 1254 1291 1286 1268 1278 + Eam1105I GACNNNNNGTC 1273 1272 . . 1268 1278 - Hin4I GAYNNNNNVTC 1291 1286 1259 1254 1278 1282 + BccI CCATC 1286 1287 . . 1278 1288 + BsiYI* CCNNNNNNNGG 1284 1281 . . 1282 1286 + BssKI CCNGG 1281 1286 . . 1282 1286 + BseBI CCWGG 1283 1284 . . 1282 1287 + SecI* CCNNGG 1282 1286 . . 1282 1286 + ScrFI CCNGG 1283 1284 . . 1282 1286 + EcoRII CCWGG 1281 1286 . . 1279 1283 - FokI GGATG 1265 1269 . . 1279 1283 - BseGI GGATG 1278 1276 . . 1312 1316 + MboII GAAGA 1324 1323 . . 1317 1320 + CviRI* TGCA 1318 1318 . . 1315 1319 - SfaNI GCATC 1305 1309 . . 1322 1326 + MboII GAAGA 1334 1333 . . 1328 1331 + SetI ASST 1331 1327 . . 1330 1334 + DdeI CTNAG 1330 1333 . . 1339 1343 + HphI GGTGA 1351 1350 . . 1342 1345 + DpnI GATC 1343 1343 . . 1342 1345 + BstKTI GATC 1344 1342 . . 1342 1345 + MboI GATC 1341 1345 . . 1348 1352 + TspDTI ATGAA 1363 1361 . . 1355 1360 + TsoI TARCCA 1371 1369 . . 1356 1359 + CviJI RGCY 1357 1357 . . 1362 1367 + ApoI RAATTY 1362 1366 . . 1363 1366 + TspEI AATT 1362 1366 . . 1371 1374 + TspEI AATT 1370 1374 . . 1375 1379 + HphI GGTGA 1387 1386 . . 1383 1387 + Hpy99I CGWCG 1387 1382 . . 1385 1388 + TaqI TCGA 1385 1387 . . 1399 1403 + MboII GAAGA 1411 1410 . . 1399 1404 + BbvII* GAAGAC 1406 1410 . . 1403 1407 + Tsp4CI* ACNGT 1405 1404 . . 1405 1409 + HphI GGTGA 1417 1416 . . 1413 1417 + BmgT120I GGNCC 1414 1415 . . 1413 1417 + AvaII GGWCC 1413 1416 . . 1413 1417 + AsuI* GGNCC 1413 1416 . . 1424 1428 + Tsp4CI* ACNGT 1426 1425 . . 1427 1432 + Hpy166II GTNNAC 1429 1429 . . 1427 1432 + HindII GTYRAC 1429 1429 . . 1432 1436 + BccI CCATC 1440 1441 . . 1439 1442 + CviRI* TGCA 1440 1440 . . 1452 1455 + TspEI AATT 1451 1455 . . 1469 1473 + DdeI CTNAG 1469 1472 . . 1473 1477 + TfiI GAWTC 1473 1476 . . 1473 1477 + HinfI GANTC 1473 1476 . . 1480 1485 + StyI CCWWGG 1480 1484 . . 1480 1485 + SecI* CCNNGG 1480 1484 . . 1489 1492 + SetI ASST 1492 1488 . . 1498 1501 + MseI TTAA 1498 1500 . . 1502 1506 + TspDTI ATGAA 1517 1515 . . 1513 1516 + CviRI* TGCA 1514 1514 . . 1518 1521 + SetI ASST 1521 1517 . . 1521 1526 + PsiI TTATAA 1523 1523 . . 1535 1538 + TaqI TCGA 1535 1537 . . 1536 1540 + Hpy99I CGWCG 1540 1535 . . 1538 1542 + Tsp4CI* ACNGT 1540 1539 . . 1540 1544 + HphI GGTGA 1552 1551 . . 1541 1545 + Tsp45I GTSAC 1540 1545 . . 1541 1545 + MaeIII GTNAC 1540 1545 . . 1549 1553 + TspDTI ATGAA 1564 1562 . . 1551 1556 + ApoI RAATTY 1551 1555 . . 1552 1555 + TspEI AATT 1551 1555 . . 1556 1559 + CviRI* TGCA 1557 1557 . . 1557 1562 + NspI RCATGY 1561 1557 . . 1558 1561 + FatI CATG 1557 1561 . . 1558 1561 + NlaIII CATG 1561 1557 . . 1558 1561 + CviAII CATG 1558 1560 . . 1573 1577 + MboII GAAGA 1585 1584 . . 1578 1581 + CviJI RGCY 1579 1579 . . 1573 1578 - Ksp632I* CTCTTC 1568 1571 . . 1576 1579 - MnlI CCTC 1569 1568 . . 1583 1588 + BsgI GTGCAG 1604 1602 . . 1584 1587 + CviRI* TGCA 1585 1585 . . 1580 1585 - BsiI* CACGAG 1580 1584 . . 1590 1593 + SetI ASST 1593 1589 . . 1590 1593 + CviJI RGCY 1591 1591 . . 1590 1593 + AluI AGCT 1591 1591 . . 1597 1600 + TspEI AATT 1596 1600 . . 1600 1603 + MseI TTAA 1600 1602 . . 1604 1609 - TaqII CACCCA 1594 1592 . . 1624 1627 + MseI TTAA 1624 1626 . . 1629 1632 + SetI ASST 1632 1628 . . 1630 1640 + EcoNI CCTNNNNNAGG 1634 1635 . . 1630 1640 + BsiYI* CCNNNNNNNGG 1636 1633 . . 1631 1635 + DdeI CTNAG 1631 1634 . . 1638 1641 + SetI ASST 1641 1637 . . 1639 1643 + HphI GGTGA 1651 1650 . . 1640 1645 + Hpy166II GTNNAC 1642 1642 . . 1644 1647 + SetI ASST 1647 1643 . . 1654 1658 + TauI GCSGC 1657 1654 . . 1654 1658 + BlsI GCNGC 1656 1655 . . 1654 1658 + BisI GCNGC 1655 1656 . . 1656 1659 + CviJI RGCY 1657 1657 . . 1654 1657 - AciI CCGC 1654 1656 . . 1661 1666 + SmlI CTYRAG 1661 1665 . . 1662 1667 + Hpy178III* TCNNGA 1663 1665 . . 1661 1666 - Bce83I* CTTGAG 1646 1644 . . 1669 1673 - TspDTI ATGAA 1659 1657 . . 1675 1679 + BsrI ACTGG 1680 1678 . . 1680 1684 - TspDTI ATGAA 1670 1668 . . 1687 1690 + MseI TTAA 1687 1689 . . 1702 1706 + TfiI GAWTC 1702 1705 . . 1702 1706 + HinfI GANTC 1702 1705 . . 1714 1717 + DpnI GATC 1715 1715 . . 1714 1717 + BstKTI GATC 1716 1714 . . 1714 1717 + MboI GATC 1713 1717 . . 1726 1729 + MseI TTAA 1726 1728 . . 1750 1754 + Hpy188I TCNGA 1752 1751 . . 1754 1758 + Tsp4CI* ACNGT 1756 1755 . . 1760 1763 + TaqI TCGA 1760 1762 . . 1762 1767 + SduI GDGCHC 1766 1762 . . 1762 1767 + HgiAI* GWGCWC 1766 1762 . . 1779 1782 + SetI ASST 1782 1778 . . 1780 1783 + MnlI CCTC 1790 1789 . . 1790 1795 + Hpy178III* TCNNGA 1791 1793 . . 1790 1795 + BspHI TCATGA 1790 1794 . . 1791 1794 + FatI CATG 1790 1794 . . 1791 1794 + NlaIII CATG 1794 1790 . . 1791 1794 + CviAII CATG 1791 1793 . . 1792 1796 + TspDTI ATGAA 1807 1805 . . 1797 1801 + Tsp4CI* ACNGT 1799 1798 . . 1811 1815 + BmgT120I GGNCC 1812 1813 . . 1811 1815 + AvaII GGWCC 1811 1814 . . 1811 1815 + AsuI* GGNCC 1811 1814 . . 1813 1818 + Cfr10I RCCGGY 1813 1817 . . 1813 1818 + BetI* WCCGGW 1813 1817 . . 1813 1818 + AgeI ACCGGT 1813 1817 . . 1814 1817 + HpaII CCGG 1814 1816 . . 1817 1822 + Hpy166II GTNNAC 1819 1819 . . 1855 1858 + TspEI AATT 1854 1858 . . 1846 1859 - AjuI GAANNNNNNNTTGG 1871 1866 1839 1834 1861 1865 - BsrI ACTGG 1861 1859 . . 1860 1865 - BfiI ACTGGG 1855 1854 . . 1875 1879 + DdeI CTNAG 1875 1878 . . 1879 1883 + HgaI GACGC 1888 1893 . . 1881 1884 + FnuDII* CGCG 1882 1882 . . 1884 1888 + MboII GAAGA 1896 1895 . . 1903 1906 + MnlI CCTC 1913 1912 . . 1900 1904 - BslFI GGGAC 1885 1889 . . 1928 1932 + TspDTI ATGAA 1943 1941 . . 1935 1939 + Tsp45I GTSAC 1934 1939 . . 1935 1939 + MaeIII GTNAC 1934 1939 . . 1944 1948 + Hpy99I CGWCG 1948 1943 . . 1956 1959 + TspEI AATT 1955 1959 . . 1966 1969 + CviJI RGCY 1967 1967 . . 1964 1967 - MnlI CCTC 1957 1956 . . 1973 1978 + ApoI RAATTY 1973 1977 . . 1974 1977 + TspEI AATT 1973 1977 . . 1978 1981 + MseI TTAA 1978 1980 . . 1986 1990 + HphI GGTGA 1998 1997 . . 1989 1993 - BccI CCATC 1983 1984 . . 2005 2008 + MaeI CTAG 2005 2007 . . 2007 2010 + SetI ASST 2010 2006 . . 2008 2012 + HphI GGTGA 2020 2019 . . 2011 2015 - BccI CCATC 2005 2006 . . 2022 2027 - BsmI GAATGC 2022 2020 . . 2044 2047 + MseI TTAA 2044 2046 . . 2054 2058 + BceAI ACGGC 2070 2072 . . 2056 2059 + CviJI RGCY 2057 2057 . . 2056 2059 + HaeIII GGCC 2057 2057 . . 2056 2060 + BmgT120I GGNCC 2057 2058 . . 2056 2060 + AsuI* GGNCC 2056 2059 . . 2067 2070 + CviJI RGCY 2068 2068 . . 2068 2072 + TauI GCSGC 2071 2068 . . 2068 2078 + MwoI GCNNNNNNNGC 2074 2071 . . 2068 2072 + BlsI GCNGC 2070 2069 . . 2068 2072 + BisI GCNGC 2069 2070 . . 2069 2072 + AciI CCGC 2069 2071 . . 2080 2084 + TspDTI ATGAA 2095 2093 . . 2105 2108 + MaeI CTAG 2105 2107 . . 2107 2110 + SetI ASST 2110 2106 . . 2112 2116 + DdeI CTNAG 2112 2115 . . 2115 2118 + SetI ASST 2118 2114 . . 2123 2126 - MnlI CCTC 2116 2115 . . 2140 2145 + VspI ATTAAT 2141 2143 . . 2141 2144 + MseI TTAA 2141 2143 . . 2149 2153 + Tsp45I GTSAC 2148 2153 . . 2149 2153 + MaeIII GTNAC 2148 2153 . . 2153 2159 + AarI CACCTGC 2163 2167 . . 2154 2157 + SetI ASST 2157 2153 . . 2154 2159 + BspMI ACCTGC 2163 2167 . . 2156 2161 - EcoP15I CAGCAG 2128 2130 . . 2182 2186 + MboII GAAGA 2194 2193 . . 2178 2182 - Hin4II* CCTTC 2172 2171 . . 2187 2192 + SpeI ACTAGT 2187 2191 . . 2188 2191 + MaeI CTAG 2188 2190 . . 2192 2195 + TaqI TCGA 2192 2194 . . 2196 2199 + TspEI AATT 2195 2199 . . 2199 2202 + CviRI* TGCA 2200 2200 . . 2206 2215 + MslI CAYNNNNRTG 2210 2210 . . 2213 2217 + Tsp45I GTSAC 2212 2217 . . 2213 2217 + MaeIII GTNAC 2212 2217 . . 2219 2222 + TspEI AATT 2218 2222 . . 2222 2227 + BclI TGATCA 2222 2226 . . 2223 2226 + DpnI GATC 2224 2224 . . 2223 2226 + BstKTI GATC 2225 2223 . . 2223 2226 + MboI GATC 2222 2226 . . 2241 2244 + SetI ASST 2244 2240 . . 2242 2246 + HphI GGTGA 2254 2253 . . 2246 2249 + TspEI AATT 2245 2249 . . 2259 2262 + SetI ASST 2262 2258 . . 2259 2265 + SexAI ACCWGGT 2259 2264 . . 2260 2264 + BssKI CCNGG 2259 2264 . . 2260 2264 + BseBI CCWGG 2261 2262 . . 2260 2264 + ScrFI CCNGG 2261 2262 . . 2260 2264 + EcoRII CCWGG 2259 2264 . . 2266 2269 + CviRI* TGCA 2267 2267 . . 2267 2272 + BsrDI GCAATG 2274 2272 . . 2270 2274 + TspDTI ATGAA 2285 2283 . . 2272 2276 + MboII GAAGA 2284 2283 . . 2287 2300 + AjuI GAANNNNNNNTTGG 2279 2274 2311 2306 2289 2292 + SetI ASST 2292 2288 . . 2289 2292 + CviJI RGCY 2290 2290 . . 2289 2292 + AluI AGCT 2290 2290 . . 2291 2295 + DdeI CTNAG 2291 2294 . . 2303 2306 + CviJI RGCY 2304 2304 . . 2318 2322 + Hpy188I TCNGA 2320 2319 . . 2323 2327 + MboII GAAGA 2335 2334 . . 2332 2336 + HphI GGTGA 2344 2343 . . 2344 2349 + VspI ATTAAT 2345 2347 . . 2345 2348 + MseI TTAA 2345 2347 . . 2348 2352 + TspDTI ATGAA 2363 2361 . . 2351 2354 + TspEI AATT 2350 2354 . . 2359 2362 + TspEI AATT 2358 2362 . . 2364 2369 + BsmI GAATGC 2370 2368 . . 2367 2370 + CviRI* TGCA 2368 2368 . . 2373 2378 + VspI ATTAAT 2374 2376 . . 2374 2377 + MseI TTAA 2374 2376 . . 2376 2379 + TspEI AATT 2375 2379 . . 2382 2385 + CviJI RGCY 2383 2383 . . 2381 2386 - TsoI TARCCA 2371 2369 . . 2400 2403 + SetI ASST 2403 2399 . . 2401 2406 + NlaIV GGNNCC 2403 2403 . . 2410 2413 + MseI TTAA 2410 2412 . . 2424 2428 - BccI CCATC 2418 2419 . . 2430 2433 + CviJI RGCY 2431 2431 . . 2441 2446 + Hpy166II GTNNAC 2443 2443 . . 2441 2446 + HindII GTYRAC 2443 2443 . . 2448 2451 + TspEI AATT 2447 2451 . . 2469 2474 + Hpy178III* TCNNGA 2470 2472 . . 2469 2474 + BetI* WCCGGW 2469 2473 . . 2469 2474 + BspMII* TCCGGA 2469 2473 . . 2470 2473 + HpaII CCGG 2470 2472 . . 2472 2476 + FokI GGATG 2485 2489 . . 2472 2476 + BseGI GGATG 2478 2476 . . 2473 2477 - BccI CCATC 2467 2468 . . 2481 2486 + Hpy178III* TCNNGA 2482 2484 . . 2485 2488 + DpnI GATC 2486 2486 . . 2485 2488 + BstKTI GATC 2487 2485 . . 2485 2488 + MboI GATC 2484 2488 . . 2493 2497 + MaeIII GTNAC 2492 2497 . . 2497 2500 + AciI CCGC 2497 2499 . . 2511 2516 + EcoRI GAATTC 2511 2515 . . 2511 2516 + ApoI RAATTY 2511 2515 . . 2512 2515 + TspEI AATT 2511 2515 . . 2514 2519 + AsuII TTCGAA 2515 2517 . . 2515 2518 + TaqI TCGA 2515 2517 . . 2535 2538 + SetI ASST 2538 2534 . . 2544 2554 + FalI AAGNNNNNCTT 2535 2530 2567 2562 2547 2552 + ApoI RAATTY 2547 2551 . . 2548 2551 + TspEI AATT 2547 2551 . . 2544 2554 - FalI AAGNNNNNCTT 2567 2562 2535 2530 2563 2566 + CviJI RGCY 2564 2564 . . 2578 2583 + ApoI RAATTY 2578 2582 . . 2579 2582 + TspEI AATT 2578 2582 . . 2590 2593 + FatI CATG 2589 2593 . . 2590 2593 + NlaIII CATG 2593 2589 . . 2590 2593 + CviAII CATG 2590 2592 . . 2604 2609 + Hpy178III* TCNNGA 2605 2607 . . 2610 2613 + SetI ASST 2613 2609 . . 2608 2612 - Hin4II* CCTTC 2602 2601 . . 2617 2622 + Hpy166II GTNNAC 2619 2619 . . 2617 2622 + HindII GTYRAC 2619 2619 . . 2624 2629 + PvuII CAGCTG 2626 2626 . . 2624 2629 + NspBII* CMGCKG 2626 2626 . . 2625 2628 + SetI ASST 2628 2624 . . 2625 2628 + CviJI RGCY 2626 2626 . . 2625 2628 + AluI AGCT 2626 2626 . . 2630 2633 + MseI TTAA 2630 2632 . . 2636 2639 + AciI CCGC 2636 2638 . . 2639 2647 + AlwNI CAGNNNCTG 2644 2641 . . 2644 2648 + BsrI ACTGG 2649 2647 . . 2641 2645 - BsmAI GTCTC 2635 2639 . . 2648 2652 + MaeIII GTNAC 2647 2652 . . 2646 2651 - TsoI TARCCA 2636 2634 . . 2651 2656 + BspLU11I* ACATGT 2651 2655 . . 2651 2656 + NspI RCATGY 2655 2651 . . 2651 2656 + AflIII ACRYGT 2651 2655 . . 2652 2655 + FatI CATG 2651 2655 . . 2652 2655 + NlaIII CATG 2655 2651 . . 2652 2655 + CviAII CATG 2652 2654 . . 2655 2659 + BsmAI GTCTC 2660 2664 . . 2664 2669 + VspI ATTAAT 2665 2667 . . 2665 2668 + MseI TTAA 2665 2667 . . 2668 2672 + TspDTI ATGAA 2683 2681 . . 2680 2684 + MboII GAAGA 2692 2691 . . 2682 2687 + XhoII RGATCY 2682 2686 . . 2682 2687 + BglII AGATCT 2682 2686 . . 2683 2686 + DpnI GATC 2684 2684 . . 2683 2686 + BstKTI GATC 2685 2683 . . 2683 2686 + MboI GATC 2682 2686 . . 2701 2712 + DrdI GACNNNNNNGTC 2707 2705 . . 2707 2711 + Tsp4CI* ACNGT 2709 2708 . . 2711 2715 + Hpy188I TCNGA 2713 2712 . . 2721 2724 + CviJI RGCY 2722 2722 . . 2717 2722 - Eco57MI CTGRAG 2702 2700 . . 2717 2722 - Eco57I CTGAAG 2702 2700 . . 2726 2730 + Tsp4CI* ACNGT 2728 2727 . . 2736 2750 + XcmI CCANNNNNNNNNTGG 2743 2742 . . 2738 2741 + TspEI AATT 2737 2741 . . 2744 2749 + NdeI CATATG 2745 2747 . . 2752 2756 + HphI GGTGA 2764 2763 . . 2753 2757 + Tsp45I GTSAC 2752 2757 . . 2753 2757 + MaeIII GTNAC 2752 2757 . . 2763 2768 + XhoII RGATCY 2763 2767 . . 2763 2768 + NlaIV GGNNCC 2765 2765 . . 2763 2768 + BamHI GGATCC 2763 2767 . . 2763 2767 + BinI* GGATC 2771 2772 . . 2764 2767 + DpnI GATC 2765 2765 . . 2764 2767 + BstKTI GATC 2766 2764 . . 2764 2767 + MboI GATC 2763 2767 . . 2760 2765 - MmeI TCCRAC 2741 2739 . . 2767 2770 + MnlI CCTC 2777 2776 . . 2764 2768 - BinI* GGATC 2758 2759 . . 2769 2774 + Hpy178III* TCNNGA 2770 2772 . . 2781 2784 + SetI ASST 2784 2780 . . 2779 2783 - Hin4II* CCTTC 2773 2772 . . 2787 2790 + CviRI* TGCA 2788 2788 . . 2793 2798 + Cfr10I RCCGGY 2793 2797 . . 2793 2798 + BetI* WCCGGW 2793 2797 . . 2793 2798 + AgeI ACCGGT 2793 2797 . . 2794 2797 + HpaII CCGG 2794 2796 . . 2800 2803 + TspEI AATT 2799 2803 . . 2816 2820 + BslFI GGGAC 2830 2834 . . 2816 2821 + NlaIV GGNNCC 2818 2818 . . 2817 2821 + BmgT120I GGNCC 2818 2819 . . 2817 2821 + AvaII GGWCC 2817 2820 . . 2817 2821 + AsuI* GGNCC 2817 2820 . . 2821 2824 + FatI CATG 2820 2824 . . 2821 2824 + NlaIII CATG 2824 2820 . . 2821 2824 + CviAII CATG 2821 2823 . . 2822 2827 + EcoT22I ATGCAT 2826 2822 . . 2823 2826 + CviRI* TGCA 2824 2824 . . 2830 2835 + SspI AATATT 2832 2832 . . 2840 2843 + MseI TTAA 2840 2842 . . 2844 2849 + TsoI TARCCA 2860 2858 . . 2847 2857 + BsiYI* CCNNNNNNNGG 2853 2850 . . 2857 2860 + CviJI RGCY 2858 2858 . . 2871 2874 + CviRI* TGCA 2872 2872 . . 2874 2877 + TspEI AATT 2873 2877 . . 2886 2889 + CviRI* TGCA 2887 2887 . . 2895 2900 + SspI AATATT 2897 2897 . . 2902 2906 + BmgT120I GGNCC 2903 2904 . . 2902 2906 + AvaII GGWCC 2902 2905 . . 2902 2906 + AsuI* GGNCC 2902 2905 . . 2911 2914 - MnlI CCTC 2904 2903 . . 2917 2920 + SetI ASST 2920 2916 . . 2910 2920 - BsaXI ACNNNNNCTCC 2932 2929 2902 2899 2924 2928 + Hpy188I TCNGA 2926 2925 . . 2941 2945 + FokI GGATG 2954 2958 . . 2941 2945 + BseGI GGATG 2947 2945 . . 2941 2952 - TstI CACNNNNNNTCC 2965 2960 2933 2928 2959 2963 + MboII GAAGA 2971 2970 . . 2959 2964 + BbvII* GAAGAC 2966 2970 . . 2974 2977 - AciI CCGC 2974 2976 . . 2985 2989 + BinI* GGATC 2993 2994 . . 2986 2989 + DpnI GATC 2987 2987 . . 2986 2989 + BstKTI GATC 2988 2986 . . 2986 2989 + MboI GATC 2985 2989 . . 2998 3003 + NmeAIII GCCGAG 3024 3022 . . 2995 2999 - SfaNI GCATC 2985 2989 . . 3021 3026 + SmlI CTYRAG 3021 3025 . . 3021 3026 + AflII CTTAAG 3021 3025 . . 3022 3025 + MseI TTAA 3022 3024 . . 3034 3039 + VspI ATTAAT 3035 3037 . . 3035 3038 + MseI TTAA 3035 3037 . . 3057 3060 + CviJI RGCY 3058 3058 . . 3056 3061 - TsoI TARCCA 3046 3044 . . 3061 3064 + TspEI AATT 3060 3064 . . 3064 3067 + MseI TTAA 3064 3066 . . 3074 3078 + BssKI CCNGG 3073 3078 . . 3074 3078 + BseBI CCWGG 3075 3076 . . 3074 3079 + SecI* CCNNGG 3074 3078 . . 3074 3078 + ScrFI CCNGG 3075 3076 . . 3074 3078 + EcoRII CCWGG 3073 3078 . . 3087 3092 + StuI AGGCCT 3089 3089 . . 3088 3091 + CviJI RGCY 3089 3089 . . 3088 3091 + HaeIII GGCC 3089 3089 . . 3099 3102 + SetI ASST 3102 3098 . . 3100 3103 + MnlI CCTC 3110 3109 . . 3105 3108 + CviRI* TGCA 3106 3106 . . 3113 3116 + TspEI AATT 3112 3116 . . 3120 3123 + SetI ASST 3123 3119 . . 3127 3130 + DpnI GATC 3128 3128 . . 3127 3130 + BstKTI GATC 3129 3127 . . 3127 3130 + MboI GATC 3126 3130 . . 3129 3134 + Hpy178III* TCNNGA 3130 3132 . . 3127 3131 - BinI* GGATC 3121 3122 . . 3134 3138 + TspDTI ATGAA 3149 3147 . . 3136 3145 + XmnI GAANNNNTTC 3140 3140 . . 3139 3143 + Tsp4CI* ACNGT 3141 3140 . . 3144 3149 + Hpy178III* TCNNGA 3145 3147 . . 3155 3158 + MseI TTAA 3155 3157 . . 3169 3172 + CviJI RGCY 3170 3170 . . 3182 3186 + TfiI GAWTC 3182 3185 . . 3182 3186 + HinfI GANTC 3182 3185 . . 3186 3192 + TspRI CASTGNN 3192 3183 . . 3198 3202 + HphI GGTGA 3210 3209 . . 3216 3219 + TspEI AATT 3215 3219 . . 3246 3250 + BsrI ACTGG 3251 3249 . . 3247 3252 + Eco57MI CTGRAG 3268 3266 . . 3247 3252 + GsuI CTGGAG 3268 3266 . . 3249 3254 + NlaIV GGNNCC 3251 3251 . . 3251 3254 + CviJI RGCY 3252 3252 . . 3253 3257 + BssKI CCNGG 3252 3257 . . 3253 3257 + BseBI CCWGG 3254 3255 . . 3253 3257 + ScrFI CCNGG 3254 3255 . . 3253 3257 + EcoRII CCWGG 3252 3257 . . 3254 3262 + AlwNI CAGNNNCTG 3259 3256 . . 3255 3258 + SetI ASST 3258 3254 . . 3257 3260 + Csp6I GTAC 3257 3259 . . 3257 3260 + RsaI GTAC 3258 3258 . . 3262 3272 + MwoI GCNNNNNNNGC 3268 3265 . . 3271 3281 + MwoI GCNNNNNNNGC 3277 3274 . . 3278 3283 + SduI GDGCHC 3282 3278 . . 3278 3283 + HgiAI* GWGCWC 3282 3278 . . 3292 3296 + HphI GGTGA 3304 3303 . . 3293 3298 + Hpy166II GTNNAC 3295 3295 . . 3297 3303 + SexAI ACCWGGT 3297 3302 . . 3298 3302 + BssKI CCNGG 3297 3302 . . 3298 3302 + BseBI CCWGG 3299 3300 . . 3298 3302 + ScrFI CCNGG 3299 3300 . . 3298 3302 + EcoRII CCWGG 3297 3302 . . 3300 3303 + SetI ASST 3303 3299 . . 3302 3307 + Hpy166II GTNNAC 3304 3304 . . 3302 3305 + Csp6I GTAC 3302 3304 . . 3302 3305 + RsaI GTAC 3303 3303 . . 3318 3322 + MboII GAAGA 3330 3329 . . 3318 3323 + BbvII* GAAGAC 3325 3329 . . 3322 3325 + SetI ASST 3325 3321 . . 3326 3330 - TspDTI ATGAA 3316 3314 . . 3333 3336 + CviRI* TGCA 3334 3334 . . 3334 3344 + MwoI GCNNNNNNNGC 3340 3337 . . 3336 3339 + SetI ASST 3339 3335 . . 3334 3339 - BspMI ACCTGC 3325 3329 . . 3334 3340 - AarI CACCTGC 3325 3329 . . 3342 3345 + CviJI RGCY 3343 3343 . . 3348 3351 + FatI CATG 3347 3351 . . 3348 3351 + NlaIII CATG 3351 3347 . . 3348 3351 + CviAII CATG 3348 3350 . . 3349 3353 + TspDTI ATGAA 3364 3362 . . 3355 3359 + MaeIII GTNAC 3354 3359 . . 3363 3366 + SetI ASST 3366 3362 . . 3370 3373 + MnlI CCTC 3380 3379 . . 3384 3387 + TspEI AATT 3383 3387 . . 3398 3403 + StyI CCWWGG 3398 3402 . . 3398 3403 + SecI* CCNNGG 3398 3402 . . 3401 3404 + SetI ASST 3404 3400 . . 3414 3417 + SetI ASST 3417 3413 . . 3419 3424 + PsiI TTATAA 3421 3421 . . 3449 3453 + TspDTI ATGAA 3464 3462 . . 3456 3459 + CviJI RGCY 3457 3457 . . 3457 3461 + BlsI GCNGC 3459 3458 . . 3457 3461 + BisI GCNGC 3458 3459 . . 3457 3461 + TseI GCWGC 3457 3460 . . 3461 3464 + MaeI CTAG 3461 3463 . . 3457 3461 - BbvI GCAGC 3444 3448 . . 3470 3473 + MseI TTAA 3470 3472 . . 3474 3477 + SetI ASST 3477 3473 . . 3491 3497 + TspRI CASTGNN 3497 3488 . . 3491 3496 - BtsI GCAGTG 3490 3488 . . 3499 3503 + Hpy188I TCNGA 3501 3500 . . 3503 3506 + TaiI ACGT 3506 3502 . . 3503 3506 + SetI ASST 3506 3502 . . 3503 3506 + MaeII ACGT 3503 3505 . . 3507 3510 + SetI ASST 3510 3506 . . 3507 3510 + CviJI RGCY 3508 3508 . . 3507 3510 + AluI AGCT 3508 3508 . . 3526 3531 + Hpy166II GTNNAC 3528 3528 . . 3535 3545 + Hin4I GAYNNNNNVTC 3526 3521 3558 3553 3535 3545 + Eam1105I GACNNNNNGTC 3540 3539 . . 3535 3545 - Hin4I GAYNNNNNVTC 3558 3553 3526 3521 3549 3558 + MslI CAYNNNNRTG 3553 3553 . . 3547 3551 - TspDTI ATGAA 3537 3535 . . 3570 3573 + SetI ASST 3573 3569 . . 3572 3575 + Csp6I GTAC 3572 3574 . . 3572 3575 + RsaI GTAC 3573 3573 . . 3589 3594 + MfeI CAATTG 3589 3593 . . 3590 3593 + TspEI AATT 3589 3593 . . 3597 3602 + Hpy166II GTNNAC 3599 3599 . . 3597 3602 + HindII GTYRAC 3599 3599 . . 3607 3611 + MboII GAAGA 3619 3618 . . 3610 3615 + EcoRV GATATC 3612 3612 . . 3615 3618 + FnuDII* CGCG 3616 3616 . . 3616 3619 - AciI CCGC 3616 3618 . . 3626 3631 + TsoI TARCCA 3642 3640 . . 3629 3633 + BssKI CCNGG 3628 3633 . . 3629 3633 + BseBI CCWGG 3630 3631 . . 3629 3634 + SecI* CCNNGG 3629 3633 . . 3629 3633 + ScrFI CCNGG 3630 3631 . . 3629 3633 + EcoRII CCWGG 3628 3633 . . 3633 3636 + CviJI RGCY 3634 3634 . . 3641 3644 + TspEI AATT 3640 3644 . . 3654 3657 + CviJI RGCY 3655 3655 . . 3656 3659 + MnlI CCTC 3666 3665 . . 3658 3662 + Hpy188I TCNGA 3660 3659 . . 3667 3672 + SspI AATATT 3669 3669 . . 3682 3685 + DpnI GATC 3683 3683 . . 3682 3685 + BstKTI GATC 3684 3682 . . 3682 3685 + MboI GATC 3681 3685 . . 3684 3687 + TaqI TCGA 3684 3686 . . 3687 3690 + TspEI AATT 3686 3690 . . 3690 3693 + CviRI* TGCA 3691 3691 . . 3698 3701 + TaiI ACGT 3701 3697 . . 3698 3701 + SetI ASST 3701 3697 . . 3698 3701 + MaeII ACGT 3698 3700 . . 3709 3713 - Hin4II* CCTTC 3703 3702 . . 3714 3717 + Csp6I GTAC 3714 3716 . . 3714 3717 + RsaI GTAC 3715 3715 . . 3726 3731 + ClaI ATCGAT 3727 3729 . . 3727 3730 + TaqI TCGA 3727 3729 . . 3729 3734 + EcoRV GATATC 3731 3731 . . 3776 3781 + BsmI GAATGC 3782 3780 . . 3779 3782 + CviRI* TGCA 3780 3780 . . 3783 3787 + Tsp4CI* ACNGT 3785 3784 . . 3786 3790 + MaeIII GTNAC 3785 3790 . . 3789 3792 + TaiI ACGT 3792 3788 . . 3789 3792 + SetI ASST 3792 3788 . . 3789 3792 + MaeII ACGT 3789 3791 . . 3790 3794 + Hpy99I CGWCG 3794 3789 . . 3796 3799 + Hin6I GCGC 3796 3798 . . 3796 3799 + GlaI GCGC 3797 3797 . . 3796 3799 + HhaI GCGC 3798 3796 . . 3801 3806 + Hpy178III* TCNNGA 3802 3804 . . 3815 3818 + MseI TTAA 3815 3817 . . 3819 3822 + SetI ASST 3822 3818 . . 3821 3826 + Hpy166II GTNNAC 3823 3823 . . 3825 3828 + SetI ASST 3828 3824 . . 3836 3841 - BsiI* CACGAG 3836 3840 . . 3841 3844 - AciI CCGC 3841 3843 . . 3847 3852 + VspI ATTAAT 3848 3850 . . 3848 3851 + MseI TTAA 3848 3850 . . 3850 3855 + SspI AATATT 3852 3852 . . 3855 3860 + Hpy178III* TCNNGA 3856 3858 . . 3855 3860 + NruI TCGCGA 3857 3857 . . 3856 3859 + FnuDII* CGCG 3857 3857 . . 3857 3862 + BtgZI GCGATG 3872 3876 . . 3857 3867 + MwoI GCNNNNNNNGC 3863 3860 . . 3863 3867 + BceAI ACGGC 3879 3881 . . 3870 3873 - MnlI CCTC 3863 3862 . . 3875 3878 + TspEI AATT 3874 3878 . . 3888 3891 + SetI ASST 3891 3887 . . 3890 3894 + MaeIII GTNAC 3889 3894 . . 3886 3890 - Hin4II* CCTTC 3880 3879 . . 3893 3897 + BceAI ACGGC 3909 3911 . . 3895 3898 + CviJI RGCY 3896 3896 . . 3895 3898 + HaeIII GGCC 3896 3896 . . 3896 3901 + Cac8I GCNNGC 3898 3898 . . 3912 3917 + Hpy166II GTNNAC 3914 3914 . . 3913 3918 + TatI WGTACW 3913 3917 . . 3913 3918 + Bsp1407I TGTACA 3913 3917 . . 3914 3917 + Csp6I GTAC 3914 3916 . . 3914 3917 + RsaI GTAC 3915 3915 . . 3919 3923 - BccI CCATC 3913 3914 . . 3934 3939 + XbaI TCTAGA 3934 3938 . . 3934 3939 + Hpy178III* TCNNGA 3935 3937 . . 3935 3938 + MaeI CTAG 3935 3937 . . 3948 3953 + TsoI TARCCA 3964 3962 . . 3952 3955 + FatI CATG 3951 3955 . . 3952 3955 + NlaIII CATG 3955 3951 . . 3952 3955 + CviAII CATG 3952 3954 . . 3970 3974 - TspGWI ACGGA 3960 3958 . . 3983 3986 + TaqI TCGA 3983 3985 . . 3987 3990 + SetI ASST 3990 3986 . . 3987 3990 + CviJI RGCY 3988 3988 . . 3987 3990 + AluI AGCT 3988 3988 . . 3988 3992 + BlsI GCNGC 3990 3989 . . 3988 3992 + BisI GCNGC 3989 3990 . . 3988 3992 + TseI GCWGC 3988 3991 . . 3990 3993 + CviRI* TGCA 3991 3991 . . 3988 3992 - BbvI GCAGC 3975 3979 . . 4014 4017 + TspEI AATT 4013 4017 . . 4016 4019 + MseI TTAA 4016 4018 . . 4026 4029 + FatI CATG 4025 4029 . . 4026 4029 + NlaIII CATG 4029 4025 . . 4026 4029 + CviAII CATG 4026 4028 . . 4037 4041 + Tsp4CI* ACNGT 4039 4038 . . 4049 4052 + TaqI TCGA 4049 4051 . . 4051 4054 + DpnI GATC 4052 4052 . . 4051 4054 + BstKTI GATC 4053 4051 . . 4051 4054 + MboI GATC 4050 4054 . . 4044 4054 - Hin4I GAYNNNNNVTC 4067 4062 4035 4030 4055 4066 + TstI CACNNNNNNTCC 4046 4041 4078 4073 4055 4060 + BtrI CACGTC 4057 4057 . . 4051 4055 - BinI* GGATC 4045 4046 . . 4056 4059 + TaiI ACGT 4059 4055 . . 4056 4059 + SetI ASST 4059 4055 . . 4056 4059 + MaeII ACGT 4056 4058 . . 4064 4069 + MmeI TCCRAC 4089 4087 . . 4074 4077 + SetI ASST 4077 4073 . . 4075 4079 + HphI GGTGA 4087 4086 . . 4096 4100 + HphI GGTGA 4108 4107 . . 4139 4143 + BslFI GGGAC 4153 4157 . . 4137 4140 - MnlI CCTC 4130 4129 . . 4141 4145 + HinfI GANTC 4141 4144 . . 4141 4145 - PleI GAGTC 4135 4136 . . 4141 4145 - MlyI GAGTC 4135 4135 . . 4151 4154 + CviRI* TGCA 4152 4152 . . 4152 4156 + BlsI GCNGC 4154 4153 . . 4152 4156 + BisI GCNGC 4153 4154 . . 4152 4156 + BbvI GCAGC 4164 4168 . . 4152 4156 + TseI GCWGC 4152 4155 . . 4154 4157 + SetI ASST 4157 4153 . . 4154 4157 + CviJI RGCY 4155 4155 . . 4154 4157 + AluI AGCT 4155 4155 . . 4155 4160 + Cac8I GCNNGC 4157 4157 . . 4155 4160 + BmtI GCTAGC 4159 4155 . . 4155 4160 + NheI GCTAGC 4155 4159 . . 4156 4159 + MaeI CTAG 4156 4158 . . 4158 4161 + CviJI RGCY 4159 4159 . . 4160 4163 + MnlI CCTC 4170 4169 . . 4163 4167 + Hin4II* CCTTC 4173 4172 . . 4165 4170 + AsuII TTCGAA 4166 4168 . . 4166 4169 + TaqI TCGA 4166 4168 . . 4190 4193 + TaqI TCGA 4190 4192 . . 4195 4199 + TauI GCSGC 4198 4195 . . 4195 4199 + BlsI GCNGC 4197 4196 . . 4195 4199 + BisI GCNGC 4196 4197 . . 4192 4196 - SfaNI GCATC 4182 4186 . . 4197 4200 + CviJI RGCY 4198 4198 . . 4197 4200 + HaeIII GGCC 4198 4198 . . 4195 4198 - AciI CCGC 4195 4197 . . 4207 4211 + TspDTI ATGAA 4222 4220 . . 4216 4220 + HgaI GACGC 4225 4230 . . 4227 4230 + SetI ASST 4230 4226 . . 4225 4229 - Hin4II* CCTTC 4219 4218 . . 4234 4238 + Hpy188I TCNGA 4236 4235 . . 4260 4269 + BsaBI GATNNNNATC 4264 4264 . . 4264 4267 + TaqI TCGA 4264 4266 . . 4265 4270 + McrI* CGRYCG 4268 4266 . . 4265 4270 + PvuI CGATCG 4268 4266 . . 4266 4269 + DpnI GATC 4267 4267 . . 4266 4269 + BstKTI GATC 4268 4266 . . 4266 4269 + MboI GATC 4265 4269 . . 4270 4275 + KpnI GGTACC 4274 4270 . . 4270 4275 + NlaIV GGNNCC 4272 4272 . . 4270 4275 + HgiCI* GGYRCC 4270 4274 . . 4270 4275 + Acc65I GGTACC 4270 4274 . . 4271 4274 + Csp6I GTAC 4271 4273 . . 4271 4274 + RsaI GTAC 4272 4272 . . 4273 4278 + Cfr10I RCCGGY 4273 4277 . . 4273 4278 + BetI* WCCGGW 4273 4277 . . 4273 4278 + AgeI ACCGGT 4273 4277 . . 4274 4277 + HpaII CCGG 4274 4276 . . 4276 4281 + NlaIV GGNNCC 4278 4278 . . 4282 4287 + AhaIII* TTTAAA 4284 4284 . . 4283 4286 + MseI TTAA 4283 4285 . . 4292 4295 + MseI TTAA 4292 4294 . . 4296 4299 + SetI ASST 4299 4295 . . 4298 4301 + Csp6I GTAC 4298 4300 . . 4298 4301 + RsaI GTAC 4299 4299 . . 4309 4313 + Hpy188I TCNGA 4311 4310 . . 4317 4322 + XhoII RGATCY 4317 4321 . . 4317 4321 + BinI* GGATC 4325 4326 . . 4318 4321 + DpnI GATC 4319 4319 . . 4318 4321 + BstKTI GATC 4320 4318 . . 4318 4321 + MboI GATC 4317 4321 . . 4323 4328 + NlaIV GGNNCC 4325 4325 . . 4327 4333 + Bpu10I CCTNAGC 4328 4331 . . 4328 4332 + DdeI CTNAG 4328 4331 . . 4332 4337 + BtgZI GCGATG 4347 4351 . . 4349 4353 + BsmAI GTCTC 4354 4358 . . 4360 4363 - MnlI CCTC 4353 4352 . . 4363 4367 + BlsI GCNGC 4365 4364 . . 4363 4367 + BisI GCNGC 4364 4365 . . 4363 4367 + BbvI GCAGC 4375 4379 . . 4363 4367 + TseI GCWGC 4363 4366 . . 4365 4368 + SetI ASST 4368 4364 . . 4365 4368 + CviJI RGCY 4366 4366 . . 4365 4368 + AluI AGCT 4366 4366 . . 4366 4376 + MwoI GCNNNNNNNGC 4372 4369 . . 4368 4373 + AhaIII* TTTAAA 4370 4370 . . 4369 4372 + MseI TTAA 4369 4371 . . #--------------------------------------- # # # AarI: # [CACCTGC] # 1058 1162 2163 # # Acc65I: # [GGTACC] # 113 4270 # # AccI: # [GTMKAC] # 103 650 3630 # # AciI: # [CCGC] # 3 21 48 139 188 216 # 225 243 338 354 415 428 # 500 623 642 # # AclI: # [AACGTT] # 648 3735 # # AflII: # [CTTAAG] # 1362 3021 # # AflIII: # [ACRYGT] # 1732 2651 # # AgeI: # [ACCGGT] # 110 980 1480 1813 # # AhaIII*: # [TTTAAA] # 13 18 86 665 3601 # # AjuI: # [GAANNNNNNNTTGG] # 32 32 32 408 415 1392 # 2072 # # AlfI: # [GCANNNNNNTGC] # 0 0 34 454 3895 # # AluI: # [AGCT] # 17 144 167 211 259 336 # 461 480 699 727 882 # # AlwNI: # [CAGNNNCTG] # 615 1124 2644 # # ApoI: # [RAATTY] # 31 36 49 189 422 538 # 1313 1805 # # AsuI*: # [GGNCC] # 85 118 146 245 398 435 # 714 761 1481 # # AsuII: # [TTCGAA] # 217 1651 2515 # # AvaII: # [GGWCC] # 85 118 146 398 435 714 # 1006 1481 # # BalI: # [TGGCCA] # 236 4147 # # BamHI: # [GGATCC] # 1620 2763 # # BarI: # [GAAGNNNNNNTAC] # 32 792 3559 # # BbvI: # [GCAGC] # 8 54 189 211 218 435 # 531 2737 # # BbvII*: # [GAAGAC] # 359 1058 1406 1560 # # BccI: # [CCATC] # 22 49 154 165 356 413 # 470 543 765 1446 # # Bce83I*: # [CTTGAG] # 1646 2737 # # BceAI: # [ACGGC] # 30 228 474 1809 1842 # # BcgI: # [CGANNNNNNTGC] # 34 361 3988 # # BciVI: # [GTATCC] # 1220 3163 # # BclI: # [TGATCA] # 2161 2222 # # BetI*: # [WCCGGW] # 110 324 354 656 667 792 # 1480 # # BfiI: # [ACTGGG] # 78 1777 2528 # # BglII: # [AGATCT] # 111 1701 2571 # # BinI*: # [GGATC] # 13 58 128 222 280 924 # 2758 # # BisI: # [GCNGC] # 3 18 19 43 68 164 # 168 200 308 414 432 531 # 626 1389 # # BlsI: # [GCNGC] # 3 18 18 43 69 164 # 168 200 308 414 432 531 # 626 1389 # # BmgT120I: # [GGNCC] # 85 118 146 245 398 436 # 714 761 1480 # # BmtI: # [GCTAGC] # 224 4159 # # Bpu10I: # [CCTNAGC] # 55 4328 # # BsaBI: # [GATNNNNATC] # 119 665 3599 # # BsaXI: # [ACNNNNNCTCC] # 30 1451 2902 # # BseBI: # [CCWGG] # 45 179 270 331 389 624 # 753 814 978 # # BseGI: # [GGATG] # 260 355 469 663 1200 1436 # # BseMII: # [CTCAG] # 6 998 3379 # # BsgI: # [GTGCAG] # 1604 2779 # # BsiI*: # [CACGAG] # 547 1580 2256 # # BsiYI*: # [CCNNNNNNNGG] # 1 32 139 233 352 879 # 1217 1530 # # BslFI: # [GGGAC] # 230 720 945 1165 1323 # # BsmAI: # [GTCTC] # 25 29 183 571 1694 1881 # # BsmI: # [GAATGC] # 348 601 1412 2022 # # Bsp1407I: # [TGTACA] # 470 974 2939 # # BspCNI: # [CTCAG] # 6 997 3380 # # BspHI: # [TCATGA] # 1790 2593 # # BspLU11I*: # [ACATGT] # 1732 2651 # # BspMI: # [ACCTGC] # 1058 1162 2163 # # BspMII*: # [TCCGGA] # 1146 1323 1914 # # BsrBI: # [CCGCTC] # 571 3812 # # BsrDI: # [GCAATG] # 977 1297 2109 # # BsrI: # [ACTGG] # 84 104 181 602 788 1132 # 1492 # # BssKI: # [CCNGG] # 45 179 270 331 387 624 # 755 814 978 # # BstKTI: # [GATC] # 21 52 63 81 114 141 # 156 198 215 222 262 369 # 372 509 555 1053 # # BtgZI: # [GCGATG] # 36 475 1018 2854 # # BtrI: # [CACGTC] # 326 4057 # # BtsI: # [GCAGTG] # 893 3490 # # Cac8I: # [GCNNGC] # 226 259 3898 # # Cfr10I: # [RCCGGY] # 110 901 912 980 1480 # # CfrI: # [YGGCCR] # 234 4149 # # ClaI: # [ATCGAT] # 656 3727 # # Csp6I: # [GTAC] # 27 45 85 142 200 270 # 282 357 693 2282 # # CviAII: # [CATG] # 62 74 141 165 169 232 # 233 357 527 604 799 1020 # # CviJI: # [RGCY] # 4 11 12 14 17 21 # 21 23 31 33 37 39 # 45 48 51 57 62 66 # 79 81 82 87 90 91 # 92 96 114 126 133 136 # 167 168 191 200 200 207 # 222 222 241 310 456 # # CviRI*: # [TGCA] # 15 27 28 36 43 48 # 63 67 74 86 89 93 # 101 107 122 123 148 161 # 202 211 219 225 228 231 # 244 357 420 615 # # DdeI: # [CTNAG] # 6 55 139 143 162 179 # 237 244 340 841 2037 # # DpnI: # [GATC] # 21 52 64 81 113 141 # 156 198 215 222 262 369 # 372 509 555 1053 # # DrdI: # [GACNNNNNNGTC] # 1676 2707 # # Eam1105I: # [GACNNNNNGTC] # 843 1273 2267 # # EciI: # [GGCGGA] # 1046 3337 # # Eco47III: # [AGCGCT] # 9 57 4317 # # Eco57I: # [CTGAAG] # 110 1681 2592 # # Eco57MI: # [CTGRAG] # 61 110 566 644 1115 1887 # # EcoNI: # [CCTNNNNNAGG] # 1634 2749 # # EcoP15I: # [CAGCAG] # 475 1653 2255 # # EcoRI: # [GAATTC] # 1872 2511 # # EcoRII: # [CCWGG] # 45 179 270 331 387 624 # 755 814 978 # # EcoRV: # [GATATC] # 119 652 3612 # # EcoT22I: # [ATGCAT] # 1557 2826 # # FalI: # [AAGNNNNNCTT] # 0 0 0 0 32 32 # 890 1613 1816 # # FatI: # [CATG] # 62 74 141 165 169 231 # 233 358 527 604 799 1020 # # FauI: # [CCCGC] # 792 3591 # # FnuDII*: # [CGCG] # 241 523 526 1359 1734 # # FokI: # [GGATG] # 260 335 469 670 1220 1429 # # GlaI: # [GCGC] # 9 57 586 3731 # # GsuI: # [CTGGAG] # 171 644 1115 2453 # # HaeII: # [RGCGCY] # 9 59 4315 # # HaeIII: # [GGCC] # 185 236 302 571 807 1032 # 1250 # # HgaI: # [GACGC] # 158 1888 2337 # # HgiAI*: # [GWGCWC] # 141 336 1101 1289 1516 # # HgiCI*: # [GGYRCC] # 113 4270 # # HhaI: # [GCGC] # 9 58 585 3731 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 32 32 # 32 316 477 1259 2235 # # Hin4II*: # [CCTTC] # 46 148 164 171 177 221 # 293 430 449 930 1354 # # Hin6I: # [GCGC] # 9 56 587 3731 # # HindII: # [GTYRAC] # 176 784 980 1014 1429 # # HindIII: # [AAGCTT] # 862 3521 # # HinfI: # [GANTC] # 100 162 229 242 385 826 # 959 1480 # # HpaII: # [CCGG] # 109 109 245 324 656 667 # 793 1480 # # HphI: # [GGTGA] # 21 22 30 36 90 94 # 99 135 234 275 347 420 # 446 783 1351 # # Hpy166II: # [GTNNAC] # 9 71 91 104 176 177 # 213 224 224 324 351 469 # 624 650 676 # # Hpy178III*: # [TCNNGA] # 10 12 15 51 54 56 # 79 85 123 128 165 193 # 240 282 296 360 448 450 # 657 679 # # Hpy188I: # [TCNGA] # 72 75 90 159 213 258 # 393 568 575 576 639 765 # # Hpy99I: # [CGWCG] # 153 386 408 589 1001 1846 # # KpnI: # [GGTACC] # 109 4274 # # Ksp632I*: # [CTCTTC] # 65 121 1382 2815 # # MaeI: # [CTAG] # 83 100 221 227 474 631 # 1273 1374 # # MaeII: # [ACGT] # 91 195 240 267 327 408 # 559 2296 # # MaeIII: # [GTNAC] # 36 64 104 105 155 208 # 214 280 319 394 431 494 # 602 977 # # MboI: # [GATC] # 21 52 66 81 111 141 # 156 198 215 222 262 369 # 372 509 555 1053 # # MboII: # [GAAGA] # 3 10 51 65 77 90 # 102 108 174 262 279 289 # 298 311 316 357 359 468 # 764 # # McrI*: # [CGRYCG] # 115 4268 # # MfeI: # [CAATTG] # 794 3589 # # MlyI: # [GAGTC] # 162 248 820 3153 # # MmeI: # [TCCRAC] # 294 1348 2741 # # MnlI: # [CCTC] # 30 40 44 44 119 123 # 127 159 183 195 197 206 # 221 245 267 270 286 405 # 561 661 # # MseI: # [TTAA] # 9 13 14 18 19 23 # 24 29 29 33 34 35 # 36 38 39 63 66 76 # 77 91 97 102 153 168 # 175 182 185 189 191 204 # 220 243 252 267 315 329 # 345 # # MslI: # [CAYNNNNRTG] # 470 830 1343 1740 # # MwoI: # [GCNNNNNNNGC] # 2 9 9 9 11 62 # 63 81 354 509 523 1194 # 1557 # # NdeI: # [CATATG] # 769 1638 1976 # # NheI: # [GCTAGC] # 228 4155 # # NlaIII: # [CATG] # 62 74 141 165 169 233 # 235 354 527 604 799 1020 # # NlaIV: # [GGNNCC] # 6 47 53 58 118 362 # 433 1021 1134 1151 # # NmeAIII: # [GCCGAG] # 1359 3024 # # NruI: # [TCGCGA] # 526 3857 # # NspBII*: # [CMGCKG] # 502 1757 2124 # # NspI: # [RCATGY] # 1094 1561 1728 # # PleI: # [GAGTC] # 162 248 820 3153 # # PsiI: # [TTATAA] # 962 1523 1898 # # PsrI: # [GAACNNNNNNTAC] # 32 312 4039 # # PstI: # [CTGCAG] # 711 3672 # # PvuI: # [CGATCG] # 115 4268 # # PvuII: # [CAGCTG] # 1757 2626 # # RsaI: # [GTAC] # 27 45 84 142 200 270 # 283 357 693 2282 # # SauI*: # [CCTNAGG] # 143 4240 # # ScrFI: # [CCNGG] # 45 179 270 331 389 624 # 753 814 978 # # SduI: # [GDGCHC] # 141 336 1101 1289 1516 # # SecI*: # [CCNNGG] # 198 231 324 754 1282 1594 # # SetI: # [ASST] # 4 6 6 8 8 9 # 13 13 14 15 15 18 # 18 21 22 27 29 29 # 30 30 35 38 39 39 # 45 49 53 54 60 63 # 63 69 69 69 69 70 # 71 72 73 74 75 80 # 87 89 90 91 96 99 # 100 100 101 111 128 135 # 135 135 136 151 156 161 # 182 208 228 # # SexAI: # [ACCWGGT] # 1011 1038 1086 1248 # # SfaNI: # [GCATC] # 35 86 151 201 232 310 # 491 1197 1680 # # SfeI*: # [CTRYAG] # 707 3676 # # SmlI: # [CTYRAG] # 1360 1362 1661 # # SpeI: # [ACTAGT] # 2187 2196 # # SspI: # [AATATT] # 65 183 531 772 2832 # # StuI: # [AGGCCT] # 807 1294 2282 # # StyI: # [CCWWGG] # 985 1480 1918 # # SwaI: # [ATTTAAAT] # 665 3718 # # TaiI: # [ACGT] # 91 195 240 267 324 411 # 559 2296 # # TaqI: # [TCGA] # 24 32 43 66 74 117 # 119 119 150 225 256 267 # 323 432 967 1169 # # TaqII: # [CACCCA] # 163 416 1015 2789 # # TatI: # [WGTACW] # 470 974 2939 # # TauI: # [GCSGC] # 21 185 414 502 508 626 # 2127 # # TfiI: # [GAWTC] # 229 385 1088 1201 1480 # # TseI: # [GCWGC] # 20 67 164 211 218 435 # 531 2737 # # TsoI: # [TARCCA] # 186 224 265 322 419 596 # 1000 1371 # # Tsp45I: # [GTSAC] # 36 64 208 214 394 540 # 1296 1631 # # Tsp4CI*: # [ACNGT] # 19 21 43 108 114 117 # 154 171 174 216 254 268 # 344 413 413 644 910 # # TspDTI: # [ATGAA] # 11 16 47 48 73 78 # 95 100 136 137 152 154 # 161 167 190 320 356 373 # 466 618 685 # # TspEI: # [AATT] # 4 8 8 9 17 18 # 23 27 31 35 36 45 # 46 49 51 52 52 62 # 64 72 74 81 100 101 # 103 105 106 111 139 145 # 159 168 187 188 204 206 # 222 258 299 348 370 # # TspGWI: # [ACGGA] # 82 423 801 3077 # # TspRI: # [CASTGNN] # 305 677 886 2515 # # TstI: # [CACNNNNNNTCC] # 32 32 305 1081 2933 # # Tth111I: # [GACNNNGTC] # 106 885 3392 # # VspI: # [ATTAAT] # 29 204 291 370 535 813 # 886 1255 # # XbaI: # [TCTAGA] # 449 3934 # # XcmI: # [CCANNNNNNNNNTGG] # 1640 2743 # # XhoII: # [RGATCY] # 66 81 111 1554 2571 # # XmnI: # [GAANNNNTTC] # 1243 3140 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 4383 # Reported_sequences: 1 # Reported_hitcount: 986 #---------------------------------------