Restriction Fragments for RKI1/YOR095C



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The currently selected gene/sequence is :

RKI1/YOR095C on chromosome XV from coordinates 504328 to 503552.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.14801.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 6 + CviJI RGCY 4 4 . . 4 8 + BlsI GCNGC 6 5 . . 4 8 + BisI GCNGC 5 6 . . 4 8 + TseI GCWGC 4 7 . . 7 12 + Cfr10I RCCGGY 7 11 . . 8 11 + HpaII CCGG 8 10 . . 21 24 + TspEI AATT 20 24 . . 25 29 - SfaNI GCATC 15 19 . . 34 38 + TfiI GAWTC 34 37 . . 34 38 + HinfI GANTC 34 37 . . 57 61 + FokI GGATG 70 74 . . 57 61 + BseGI GGATG 63 61 . . 55 58 - MnlI CCTC 48 47 . . 61 71 + MwoI GCNNNNNNNGC 67 64 . . 58 62 - SfaNI GCATC 48 52 . . 69 72 + SetI ASST 72 68 . . 69 72 + CviJI RGCY 70 70 . . 69 72 + AluI AGCT 70 70 . . 70 74 + BlsI GCNGC 72 71 . . 70 74 + BisI GCNGC 71 72 . . 70 74 + TseI GCWGC 70 73 . . 71 76 + PstI CTGCAG 75 71 . . 71 76 + SfeI* CTRYAG 71 75 . . 72 75 + CviRI* TGCA 73 73 . . 73 77 + BlsI GCNGC 75 74 . . 73 77 + BisI GCNGC 74 75 . . 73 77 + BbvI GCAGC 85 89 . . 73 77 + TseI GCWGC 73 76 . . 70 74 - BbvI GCAGC 57 61 . . 76 86 + MwoI GCNNNNNNNGC 82 79 . . 92 96 + TspDTI ATGAA 107 105 . . 96 101 + ApoI RAATTY 96 100 . . 97 100 + TspEI AATT 96 100 . . 99 104 + AhaIII* TTTAAA 101 101 . . 100 103 + MseI TTAA 100 102 . . 103 108 + ApoI RAATTY 103 107 . . 104 107 + TspEI AATT 103 107 . . 111 116 + BclI TGATCA 111 115 . . 112 115 + DpnI GATC 113 113 . . 112 115 + BstKTI GATC 114 112 . . 112 115 + MboI GATC 111 115 . . 120 123 + TspEI AATT 119 123 . . 129 132 + TspEI AATT 128 132 . . 145 149 + Tsp4CI* ACNGT 147 146 . . 146 152 + TspRI CASTGNN 152 143 . . 168 171 + TspEI AATT 167 171 . . 176 181 + SspI AATATT 178 178 . . 182 185 + CviRI* TGCA 183 183 . . 184 187 + FatI CATG 183 187 . . 184 187 + NlaIII CATG 187 183 . . 184 187 + CviAII CATG 184 186 . . 193 198 + ApoI RAATTY 193 197 . . 194 197 + TspEI AATT 193 197 . . 200 204 + TspDTI ATGAA 215 213 . . 208 212 - HgaI GACGC 197 202 . . 214 219 + ApoI RAATTY 214 218 . . 215 218 + TspEI AATT 214 218 . . 217 221 - TspDTI ATGAA 207 205 . . 223 226 + CviRI* TGCA 224 224 . . 228 233 + MmeI TCCRAC 253 251 . . 224 229 - BsmI GAATGC 224 222 . . 236 240 + TfiI GAWTC 236 239 . . 236 240 + HinfI GANTC 236 239 . . 236 245 + BsaBI GATNNNNATC 240 240 . . 244 249 + Hpy178III* TCNNGA 245 247 . . 272 275 + CviRI* TGCA 273 273 . . 275 278 + TspEI AATT 274 278 . . 280 283 + CviJI RGCY 281 281 . . 280 285 + NlaIV GGNNCC 282 282 . . 291 295 + Tsp4CI* ACNGT 293 292 . . 294 299 + BciVI GTATCC 305 304 . . 298 301 + MnlI CCTC 308 307 . . 320 324 + Tsp4CI* ACNGT 322 321 . . 329 333 + TspDTI ATGAA 344 342 . . 336 340 + FokI GGATG 349 353 . . 336 340 + BseGI GGATG 342 340 . . 342 347 + ApoI RAATTY 342 346 . . 343 346 + TspEI AATT 342 346 . . 350 353 + TspEI AATT 349 353 . . 351 356 + VspI ATTAAT 352 354 . . 352 359 + PacI TTAATTAA 356 354 . . 352 355 + MseI TTAA 352 354 . . 354 357 + TspEI AATT 353 357 . . 356 359 + MseI TTAA 356 358 . . 360 363 + SetI ASST 363 359 . . 381 386 + Hpy178III* TCNNGA 382 384 . . 389 392 + TspEI AATT 388 392 . . 392 397 - TsoI TARCCA 382 380 . . 397 402 + TatI WGTACW 397 401 . . 397 402 + ScaI AGTACT 399 399 . . 398 401 + Csp6I GTAC 398 400 . . 398 401 + RsaI GTAC 399 399 . . 400 405 + SpeI ACTAGT 400 404 . . 401 404 + MaeI CTAG 401 403 . . 412 415 + SetI ASST 415 411 . . 413 417 + Hin4II* CCTTC 423 422 . . 415 419 - TspDTI ATGAA 405 403 . . 430 434 + TfiI GAWTC 430 433 . . 430 434 + HinfI GANTC 430 433 . . 433 438 + Hpy178III* TCNNGA 434 436 . . 444 448 + Tsp45I GTSAC 443 448 . . 444 448 + MaeIII GTNAC 443 448 . . 445 449 - HphI GGTGA 437 436 . . 459 462 + SetI ASST 462 458 . . 467 471 + BsrI ACTGG 472 470 . . 468 473 + Eco57MI CTGRAG 489 487 . . 468 473 + GsuI CTGGAG 489 487 . . 471 474 - MnlI CCTC 464 463 . . 477 480 + SetI ASST 480 476 . . 470 480 - BsaXI ACNNNNNCTCC 492 489 462 459 470 481 - TstI CACNNNNNNTCC 494 489 462 457 492 495 + TspEI AATT 491 495 . . 496 499 + Csp6I GTAC 496 498 . . 496 499 + RsaI GTAC 497 497 . . 498 501 + SetI ASST 501 497 . . 499 503 + Hin4II* CCTTC 509 508 . . 503 506 + MnlI CCTC 513 512 . . 508 513 + BsaAI YACGTR 510 510 . . 509 512 + TaiI ACGT 512 508 . . 509 512 + SetI ASST 512 508 . . 509 512 + MaeII ACGT 509 511 . . 513 516 - MnlI CCTC 506 505 . . 518 523 + Hpy178III* TCNNGA 519 521 . . 526 529 + DpnI GATC 527 527 . . 526 529 + BstKTI GATC 528 526 . . 526 529 + MboI GATC 525 529 . . 538 543 + MfeI CAATTG 538 542 . . 539 542 + TspEI AATT 538 542 . . 542 545 + CviRI* TGCA 543 543 . . 543 548 + NspI RCATGY 547 543 . . 543 548 + Cac8I GCNNGC 545 545 . . 543 548 + SphI GCATGC 547 543 . . 544 547 + FatI CATG 543 547 . . 544 547 + NlaIII CATG 547 543 . . 544 547 + CviAII CATG 544 546 . . 556 561 + Hpy166II GTNNAC 558 558 . . 556 561 + HindII GTYRAC 558 558 . . 563 567 + Hpy188I TCNGA 565 564 . . 573 576 + SetI ASST 576 572 . . 572 575 - MnlI CCTC 565 564 . . 587 595 + AlwNI CAGNNNCTG 592 589 . . 588 591 + SetI ASST 591 587 . . 588 594 + PpuMI RGGWCCY 589 592 . . 588 594 + DraII RGGNCCY 589 592 . . 589 593 + BmgT120I GGNCC 590 591 . . 589 593 + AvaII GGWCC 589 592 . . 589 593 + AsuI* GGNCC 589 592 . . 586 591 - BspMI ACCTGC 577 581 . . 598 602 + MaeIII GTNAC 597 602 . . 616 620 - TspDTI ATGAA 606 604 . . 622 627 + ClaI ATCGAT 623 625 . . 623 626 + TaqI TCGA 623 625 . . 625 629 - SfaNI GCATC 615 619 . . 628 631 - AciI CCGC 628 630 . . 637 641 + HphI GGTGA 649 648 . . 641 646 + ApoI RAATTY 641 645 . . 642 645 + TspEI AATT 641 645 . . 646 650 + Hpy188I TCNGA 648 647 . . 649 652 + DpnI GATC 650 650 . . 649 652 + BstKTI GATC 651 649 . . 649 652 + MboI GATC 648 652 . . 649 653 - BinI* GGATC 643 644 . . 659 662 + TspEI AATT 658 662 . . 662 665 + CviRI* TGCA 663 663 . . 678 682 + Tsp4CI* ACNGT 680 679 . . 702 705 + SetI ASST 705 701 . . 709 713 - TspDTI ATGAA 699 697 . . 713 716 + TaqI TCGA 713 715 . . 721 731 + MwoI GCNNNNNNNGC 727 724 . . 729 732 + CviJI RGCY 730 730 . . 742 745 + TspEI AATT 741 745 . . 745 749 + Hpy188I TCNGA 747 746 . . 749 753 + Tsp4CI* ACNGT 751 750 . . 763 767 + MaeIII GTNAC 762 767 . . #--------------------------------------- # # # AciI: # [CCGC] # 149 628 # # AhaIII*: # [TTTAAA] # 101 676 # # AluI: # [AGCT] # 70 707 # # AlwNI: # [CAGNNNCTG] # 185 592 # # ApoI: # [RAATTY] # 7 21 90 96 128 136 # 299 # # AsuI*: # [GGNCC] # 188 589 # # AvaII: # [GGWCC] # 188 589 # # BbvI: # [GCAGC] # 28 57 692 # # BciVI: # [GTATCC] # 305 472 # # BclI: # [TGATCA] # 111 666 # # BinI*: # [GGATC] # 134 643 # # BisI: # [GCNGC] # 3 5 66 703 # # BlsI: # [GCNGC] # 3 6 66 702 # # BmgT120I: # [GGNCC] # 187 590 # # BsaAI: # [YACGTR] # 267 510 # # BsaBI: # [GATNNNNATC] # 240 537 # # BsaXI: # [ACNNNNNCTCC] # 30 285 462 # # BseGI: # [GGATG] # 63 279 435 # # BsmI: # [GAATGC] # 224 553 # # BspMI: # [ACCTGC] # 200 577 # # BsrI: # [ACTGG] # 305 472 # # BstKTI: # [GATC] # 114 123 126 414 # # Cac8I: # [GCNNGC] # 232 545 # # Cfr10I: # [RCCGGY] # 7 770 # # ClaI: # [ATCGAT] # 154 623 # # Csp6I: # [GTAC] # 98 281 398 # # CviAII: # [CATG] # 184 233 360 # # CviJI: # [RGCY] # 4 47 66 211 449 # # CviRI*: # [TGCA] # 41 49 73 110 114 120 # 270 # # DpnI: # [GATC] # 113 123 127 414 # # DraII: # [RGGNCCY] # 188 589 # # Eco57MI: # [CTGRAG] # 288 489 # # FatI: # [CATG] # 183 234 360 # # FokI: # [GGATG] # 70 279 428 # # GsuI: # [CTGGAG] # 288 489 # # HgaI: # [GACGC] # 197 580 # # Hin4II*: # [CCTTC] # 86 268 423 # # HindII: # [GTYRAC] # 219 558 # # HinfI: # [GANTC] # 34 194 202 347 # # HpaII: # [CCGG] # 8 769 # # HphI: # [GGTGA] # 128 212 437 # # Hpy166II: # [GTNNAC] # 219 558 # # Hpy178III*: # [TCNNGA] # 52 85 137 245 258 # # Hpy188I: # [TCNGA] # 30 83 99 565 # # MaeI: # [CTAG] # 376 401 # # MaeII: # [ACGT] # 268 509 # # MaeIII: # [GTNAC] # 15 154 165 443 # # MboI: # [GATC] # 111 123 129 414 # # MfeI: # [CAATTG] # 239 538 # # MmeI: # [TCCRAC] # 253 524 # # MnlI: # [CCTC] # 7 42 48 52 156 212 # 260 # # MseI: # [TTAA] # 4 100 252 421 # # MwoI: # [GCNNNNNNNGC] # 15 50 67 645 # # NlaIII: # [CATG] # 187 230 360 # # NlaIV: # [GGNNCC] # 282 495 # # NspI: # [RCATGY] # 230 547 # # PacI: # [TTAATTAA] # 356 421 # # PpuMI: # [RGGWCCY] # 188 589 # # PstI: # [CTGCAG] # 75 702 # # RsaI: # [GTAC] # 98 280 399 # # ScaI: # [AGTACT] # 378 399 # # SetI: # [ASST] # 11 15 18 21 47 52 # 64 72 72 114 291 # # SfaNI: # [GCATC] # 15 33 162 567 # # SfeI*: # [CTRYAG] # 71 706 # # SpeI: # [ACTAGT] # 377 400 # # SphI: # [GCATGC] # 230 547 # # SspI: # [AATATT] # 178 599 # # TaiI: # [ACGT] # 265 512 # # TaqI: # [TCGA] # 64 90 623 # # TatI: # [WGTACW] # 380 397 # # TfiI: # [GAWTC] # 34 194 202 347 # # TseI: # [GCWGC] # 3 4 66 704 # # TsoI: # [TARCCA] # 382 395 # # Tsp45I: # [GTSAC] # 334 443 # # Tsp4CI*: # [ACNGT] # 26 29 71 146 147 358 # # TspDTI: # [ATGAA] # 8 61 78 93 100 107 # 129 201 # # TspEI: # [AATT] # 4 7 7 9 16 17 # 20 21 26 35 36 39 # 47 60 68 76 83 103 # 103 # # TspRI: # [CASTGNN] # 152 625 # # TstI: # [CACNNNNNNTCC] # 32 283 462 # # VspI: # [ATTAAT] # 352 425 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 777 # Reported_sequences: 1 # Reported_hitcount: 174 #---------------------------------------