Restriction Fragments for HST3/YOR025W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

HST3/YOR025W on chromosome XV from coordinates 378219 to 379562.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.13799.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 17 20 + MnlI CCTC 27 26 . . 23 29 + AarI CACCTGC 33 37 . . 24 27 + SetI ASST 27 23 . . 24 29 + BspMI ACCTGC 33 37 . . 33 36 + TaqI TCGA 33 35 . . 29 33 - BsrI ACTGG 29 27 . . 34 39 + McrI* CGRYCG 37 35 . . 34 39 + PvuI CGATCG 37 35 . . 35 38 + DpnI GATC 36 36 . . 35 38 + BstKTI GATC 37 35 . . 35 38 + MboI GATC 34 38 . . 39 44 + SduI GDGCHC 43 39 . . 39 44 + HgiJII* GRGCYC 43 39 . . 40 43 + CviJI RGCY 41 41 . . 41 51 + MwoI GCNNNNNNNGC 47 44 . . 48 53 + SduI GDGCHC 52 48 . . 48 53 + HgiAI* GWGCWC 52 48 . . 52 57 + MmeI TCCRAC 77 75 . . 52 56 + Hpy188I TCNGA 54 53 . . 55 65 + Hin4I GAYNNNNNVTC 46 41 78 73 55 65 + Eam1105I GACNNNNNGTC 60 59 . . 60 64 + Tsp4CI* ACNGT 62 61 . . 65 68 + MnlI CCTC 75 74 . . 55 65 - Hin4I GAYNNNNNVTC 78 73 46 41 71 74 + CviRI* TGCA 72 72 . . 77 81 + DdeI CTNAG 77 80 . . 77 81 - BspCNI CTCAG 69 67 . . 77 81 - BseMII CTCAG 68 66 . . 84 88 + BsrI ACTGG 89 87 . . 99 102 + SetI ASST 102 98 . . 102 107 + Hpy178III* TCNNGA 103 105 . . 106 110 - SfaNI GCATC 96 100 . . 111 115 + Hpy99I CGWCG 115 110 . . 116 120 + TspDTI ATGAA 131 129 . . 126 129 + HpaII CCGG 126 128 . . 129 132 + Hin6I GCGC 129 131 . . 129 132 + GlaI GCGC 130 130 . . 129 132 + HhaI GCGC 131 129 . . 130 133 + FnuDII* CGCG 131 131 . . 133 137 + MaeIII GTNAC 132 137 . . 134 139 + TsoI TARCCA 150 148 . . 151 156 + XhoII RGATCY 151 155 . . 151 156 + BglII AGATCT 151 155 . . 152 155 + DpnI GATC 153 153 . . 152 155 + BstKTI GATC 154 152 . . 152 155 + MboI GATC 151 155 . . 155 158 + MaeI CTAG 155 157 . . 162 165 + TspEI AATT 161 165 . . 171 175 + Hpy188I TCNGA 173 172 . . 175 179 + BsrI ACTGG 180 178 . . 175 180 + BfiI ACTGGG 185 184 . . 181 186 + Cac8I GCNNGC 183 183 . . 185 195 + MwoI GCNNNNNNNGC 191 188 . . 185 195 + BstAPI GCANNNNNTGC 191 188 . . 181 192 - BcgI CGANNNNNNTGC 204 202 170 168 193 196 + CviRI* TGCA 194 194 . . 194 204 + MwoI GCNNNNNNNGC 200 197 . . 198 201 + FnuDII* CGCG 199 199 . . 199 204 + Cac8I GCNNGC 201 201 . . 199 202 - AciI CCGC 199 201 . . 199 203 - FauI CCCGC 192 194 . . 207 212 + Hpy178III* TCNNGA 208 210 . . 203 208 - BsmI GAATGC 203 201 . . 211 221 + Hin4I GAYNNNNNVTC 202 197 234 229 218 224 + SapI GCTCTTC 225 228 . . 219 224 + Ksp632I* CTCTTC 225 228 . . 223 227 + Hpy188I TCNGA 225 224 . . 220 224 - MboII GAAGA 212 211 . . 229 234 + SduI GDGCHC 233 229 . . 229 234 + HgiJII* GRGCYC 233 229 . . 230 233 + CviJI RGCY 231 231 . . 226 230 - BccI CCATC 220 221 . . 239 242 + SetI ASST 242 238 . . 241 244 + MaeI CTAG 241 243 . . 257 262 + Hpy166II GTNNAC 259 259 . . 261 265 + Tsp4CI* ACNGT 263 262 . . 281 284 + HpaII CCGG 281 283 . . 343 348 + ApoI RAATTY 343 347 . . 344 347 + TspEI AATT 343 347 . . 356 359 + CviJI RGCY 357 357 . . 354 357 - MnlI CCTC 347 346 . . 373 378 + MfeI CAATTG 373 377 . . 374 377 + TspEI AATT 373 377 . . 383 386 + CviJI RGCY 384 384 . . 389 393 + DdeI CTNAG 389 392 . . 393 397 + HgaI GACGC 402 407 . . 403 407 - TspDTI ATGAA 393 391 . . 408 413 + MstI* TGCGCA 410 410 . . 409 412 + Hin6I GCGC 409 411 . . 409 412 + GlaI GCGC 410 410 . . 409 412 + HhaI GCGC 411 409 . . 405 410 - BsrDI GCAATG 404 402 . . 411 415 + SfaNI GCATC 420 424 . . 435 439 + BlsI GCNGC 437 436 . . 435 439 + BisI GCNGC 436 437 . . 435 439 + TseI GCWGC 435 438 . . 438 441 + Hin6I GCGC 438 440 . . 438 441 + GlaI GCGC 439 439 . . 433 438 - EcoP15I CAGCAG 405 407 . . 438 441 + HhaI GCGC 440 438 . . 435 439 - BbvI GCAGC 422 426 . . 443 447 + MaeIII GTNAC 442 447 . . 457 462 + ClaI ATCGAT 458 460 . . 458 461 + TaqI TCGA 458 460 . . 463 468 + SduI GDGCHC 467 463 . . 463 468 + HgiJII* GRGCYC 467 463 . . 464 467 + CviJI RGCY 465 465 . . 460 464 - BccI CCATC 454 455 . . 467 470 + TaqI TCGA 467 469 . . 469 473 + MboII GAAGA 481 480 . . 503 506 + TspEI AATT 502 506 . . 508 511 + AciI CCGC 508 510 . . 514 517 + SetI ASST 517 513 . . 515 518 + MnlI CCTC 525 524 . . 524 529 + Hpy166II GTNNAC 526 526 . . 540 545 + Hpy178III* TCNNGA 541 543 . . 543 547 + FokI GGATG 556 560 . . 543 547 + BseGI GGATG 549 547 . . 557 560 + CviRI* TGCA 558 558 . . 560 564 + BceAI ACGGC 576 578 . . 566 569 + SetI ASST 569 565 . . 582 587 + Hpy166II GTNNAC 584 584 . . 583 588 + TatI WGTACW 583 587 . . 583 588 + Bsp1407I TGTACA 583 587 . . 584 587 + Csp6I GTAC 584 586 . . 584 587 + RsaI GTAC 585 585 . . 596 601 + Hpy178III* TCNNGA 597 599 . . 607 612 + SecI* CCNNGG 607 611 . . 608 618 + EcoNI CCTNNNNNAGG 612 613 . . 608 612 + BssKI CCNGG 607 612 . . 608 612 + BseBI CCWGG 609 610 . . 608 612 + ScrFI CCNGG 609 610 . . 608 612 + EcoRII CCWGG 607 612 . . 608 618 + BsiYI* CCNNNNNNNGG 614 611 . . 609 614 + Eco57MI CTGRAG 630 628 . . 609 614 + GsuI CTGGAG 630 628 . . 615 623 + AlwNI CAGNNNCTG 620 617 . . 616 619 + SetI ASST 619 615 . . 618 621 + Csp6I GTAC 618 620 . . 618 621 + RsaI GTAC 619 619 . . 614 619 - BspMI ACCTGC 605 609 . . 620 624 + BsrI ACTGG 625 623 . . 623 628 + Eco31I GGTCTC 629 633 . . 624 628 + BsmAI GTCTC 629 633 . . 634 638 + DdeI CTNAG 634 637 . . 638 642 + MboII GAAGA 650 649 . . 642 645 + SetI ASST 645 641 . . 638 643 - Ksp632I* CTCTTC 633 636 . . 643 647 + HphI GGTGA 655 654 . . 641 644 - MnlI CCTC 634 633 . . 648 652 + MaeIII GTNAC 647 652 . . 660 665 + Hpy178III* TCNNGA 661 663 . . 660 665 + BetI* WCCGGW 660 664 . . 660 665 + BspMII* TCCGGA 660 664 . . 661 664 + HpaII CCGG 661 663 . . 695 699 + TspDTI ATGAA 710 708 . . 697 701 - Hin4II* CCTTC 691 690 . . 733 737 + DdeI CTNAG 733 736 . . 737 747 + Hin4I GAYNNNNNVTC 728 723 760 755 738 741 + SetI ASST 741 737 . . 737 747 - Hin4I GAYNNNNNVTC 760 755 728 723 757 761 + HphI GGTGA 769 768 . . 765 769 + BccI CCATC 773 774 . . 769 773 + BccI CCATC 777 778 . . 766 770 - FokI GGATG 752 756 . . 766 770 - BseGI GGATG 765 763 . . 770 774 - FokI GGATG 756 760 . . 770 774 - BseGI GGATG 769 767 . . 780 783 + TspEI AATT 779 783 . . 792 797 + StuI AGGCCT 794 794 . . 793 796 + CviJI RGCY 794 794 . . 793 796 + HaeIII GGCC 794 794 . . 801 806 + Hpy178III* TCNNGA 802 804 . . 810 813 + TspEI AATT 809 813 . . 812 815 + MseI TTAA 812 814 . . 822 827 + Hpy178III* TCNNGA 823 825 . . 833 838 + BclI TGATCA 833 837 . . 834 837 + DpnI GATC 835 835 . . 834 837 + BstKTI GATC 836 834 . . 834 837 + MboI GATC 833 837 . . 840 843 + FatI CATG 839 843 . . 840 843 + NlaIII CATG 843 839 . . 840 843 + CviAII CATG 840 842 . . 845 848 + Csp6I GTAC 845 847 . . 845 848 + RsaI GTAC 846 846 . . 862 866 - BccI CCATC 856 857 . . 876 880 + BsrI ACTGG 881 879 . . 887 890 + TspEI AATT 886 890 . . 889 892 + MseI TTAA 889 891 . . 899 904 + ApoI RAATTY 899 903 . . 900 903 + TspEI AATT 899 903 . . 903 908 + Hpy178III* TCNNGA 904 906 . . 904 913 + MslI CAYNNNNRTG 908 908 . . 904 913 + OliI CACNNNNGTG 908 908 . . 906 911 + McrI* CGRYCG 909 907 . . 906 911 + PvuI CGATCG 909 907 . . 907 910 + DpnI GATC 908 908 . . 907 910 + BstKTI GATC 909 907 . . 907 910 + MboI GATC 906 910 . . 914 918 + TauI GCSGC 917 914 . . 914 918 + BlsI GCNGC 916 915 . . 914 918 + BisI GCNGC 915 916 . . 916 919 + CviJI RGCY 917 917 . . 916 919 + HaeIII GGCC 917 917 . . 914 917 - AciI CCGC 914 916 . . 920 925 + BclI TGATCA 920 924 . . 921 924 + DpnI GATC 922 922 . . 921 924 + BstKTI GATC 923 921 . . 921 924 + MboI GATC 920 924 . . 931 936 + Hpy166II GTNNAC 933 933 . . 941 946 + TaqII CACCCA 957 955 . . 952 956 + TfiI GAWTC 952 955 . . 952 956 + HinfI GANTC 952 955 . . 956 959 + MnlI CCTC 966 965 . . 965 969 + BceAI ACGGC 981 983 . . 990 994 + DdeI CTNAG 990 993 . . 990 994 + BspCNI CTCAG 1003 1001 . . 990 994 + BseMII CTCAG 1004 1002 . . 991 995 + Hpy188I TCNGA 993 992 . . 987 998 - CspCI CAANNNNNGTGG 1011 1009 976 974 1003 1006 + TspEI AATT 1002 1006 . . 1009 1013 + Tsp45I GTSAC 1008 1013 . . 1009 1013 + MaeIII GTNAC 1008 1013 . . 1017 1022 + Hpy178III* TCNNGA 1018 1020 . . 1021 1025 + TfiI GAWTC 1021 1024 . . 1021 1025 + HinfI GANTC 1021 1024 . . 1043 1048 + Hpy178III* TCNNGA 1044 1046 . . 1040 1044 - MboII GAAGA 1032 1031 . . 1047 1057 + Hin4I GAYNNNNNVTC 1038 1033 1070 1065 1047 1051 + HgaI GACGC 1056 1061 . . 1054 1057 + DpnI GATC 1055 1055 . . 1054 1057 + BstKTI GATC 1056 1054 . . 1054 1057 + MboI GATC 1053 1057 . . 1047 1057 - Hin4I GAYNNNNNVTC 1070 1065 1038 1033 1059 1062 + TspEI AATT 1058 1062 . . 1061 1064 + MseI TTAA 1061 1063 . . 1069 1072 + MseI TTAA 1069 1071 . . 1074 1078 + Tsp4CI* ACNGT 1076 1075 . . 1078 1081 + MseI TTAA 1078 1080 . . 1087 1090 - MnlI CCTC 1080 1079 . . 1090 1095 + Cac8I GCNNGC 1092 1092 . . 1098 1103 + SmlI CTYRAG 1098 1102 . . 1098 1103 + Bce83I* CTTGAG 1119 1117 . . 1111 1115 + TspDTI ATGAA 1126 1124 . . 1116 1119 + CviJI RGCY 1117 1117 . . 1116 1119 + HaeIII GGCC 1117 1117 . . 1116 1120 + BmgT120I GGNCC 1117 1118 . . 1116 1120 + AsuI* GGNCC 1116 1119 . . 1113 1117 - Hin4II* CCTTC 1107 1106 . . 1126 1130 + HinfI GANTC 1126 1129 . . 1126 1130 - PleI GAGTC 1120 1121 . . 1126 1130 - MlyI GAGTC 1120 1120 . . 1133 1137 + Hpy188I TCNGA 1135 1134 . . 1135 1140 + NlaIV GGNNCC 1137 1137 . . 1153 1156 + MnlI CCTC 1163 1162 . . 1166 1172 + PasI CCCWGGG 1167 1170 . . 1166 1171 + SecI* CCNNGG 1166 1170 . . 1167 1171 + BssKI CCNGG 1166 1171 . . 1167 1171 + BseBI CCWGG 1168 1169 . . 1167 1172 + SecI* CCNNGG 1167 1171 . . 1167 1171 + ScrFI CCNGG 1168 1169 . . 1167 1171 + EcoRII CCWGG 1166 1171 . . 1196 1199 + TspEI AATT 1195 1199 . . 1219 1222 + MseI TTAA 1219 1221 . . 1225 1229 + Tsp4CI* ACNGT 1227 1226 . . 1229 1232 + MseI TTAA 1229 1231 . . 1242 1246 + Tsp4CI* ACNGT 1244 1243 . . 1245 1249 + Tsp45I GTSAC 1244 1249 . . 1245 1249 + MaeIII GTNAC 1244 1249 . . 1246 1250 - HphI GGTGA 1238 1237 . . 1260 1266 + TspRI CASTGNN 1266 1257 . . 1257 1262 - Eco57MI CTGRAG 1242 1240 . . 1257 1262 - GsuI CTGGAG 1242 1240 . . 1259 1263 - BsrI ACTGG 1259 1257 . . 1264 1267 - MnlI CCTC 1257 1256 . . 1267 1271 + MboII GAAGA 1279 1278 . . 1267 1272 + BbvII* GAAGAC 1274 1278 . . 1273 1277 + MboII GAAGA 1285 1284 . . 1273 1278 - Ksp632I* CTCTTC 1268 1271 . . 1276 1279 - MnlI CCTC 1269 1268 . . 1287 1292 - BciVI GTATCC 1281 1280 . . 1300 1303 + FnuDII* CGCG 1301 1301 . . 1301 1304 + Hin6I GCGC 1301 1303 . . 1301 1304 + GlaI GCGC 1302 1302 . . 1301 1304 + HhaI GCGC 1303 1301 . . 1304 1308 + DdeI CTNAG 1304 1307 . . 1315 1325 + BsiYI* CCNNNNNNNGG 1321 1318 . . 1324 1328 + HphI GGTGA 1336 1335 . . 1325 1329 + Tsp45I GTSAC 1324 1329 . . 1325 1329 + MaeIII GTNAC 1324 1329 . . 1335 1338 + CviJI RGCY 1336 1336 . . #--------------------------------------- # # # AarI: # [CACCTGC] # 33 1311 # # AciI: # [CCGC] # 199 309 406 430 # # AlwNI: # [CAGNNNCTG] # 620 724 # # ApoI: # [RAATTY] # 343 445 556 # # AsuI*: # [GGNCC] # 228 1116 # # BbvI: # [GCAGC] # 422 922 # # BbvII*: # [GAAGAC] # 70 1274 # # BccI: # [CCATC] # 4 79 220 234 319 488 # # Bce83I*: # [CTTGAG] # 225 1119 # # BceAI: # [ACGGC] # 363 405 576 # # BcgI: # [CGANNNNNNTGC] # 34 170 1140 # # BciVI: # [GTATCC] # 63 1281 # # BclI: # [TGATCA] # 87 424 833 # # BetI*: # [WCCGGW] # 660 684 # # BfiI: # [ACTGGG] # 185 1159 # # BglII: # [AGATCT] # 151 1193 # # BisI: # [GCNGC] # 429 436 479 # # BlsI: # [GCNGC] # 428 437 479 # # BmgT120I: # [GGNCC] # 227 1117 # # BseBI: # [CCWGG] # 176 559 609 # # BseGI: # [GGATG] # 4 216 549 575 # # BseMII: # [CTCAG] # 68 340 936 # # BsiYI*: # [CCNNNNNNNGG] # 23 614 707 # # BsmAI: # [GTCTC] # 629 715 # # BsmI: # [GAATGC] # 203 1141 # # Bsp1407I: # [TGTACA] # 583 761 # # BspCNI: # [CTCAG] # 69 341 934 # # BspMI: # [ACCTGC] # 33 572 739 # # BspMII*: # [TCCGGA] # 660 684 # # BsrDI: # [GCAATG] # 404 940 # # BsrI: # [ACTGG] # 29 60 85 91 256 378 # 445 # # BssKI: # [CCNGG] # 178 559 607 # # BstAPI: # [GCANNNNNTGC] # 191 1153 # # BstKTI: # [GATC] # 14 37 73 117 133 288 # 682 # # Cac8I: # [GCNNGC] # 18 183 252 891 # # ClaI: # [ATCGAT] # 458 886 # # Csp6I: # [GTAC] # 34 227 499 584 # # CspCI: # [CAANNNNNGTGG] # 35 333 976 # # CviAII: # [CATG] # 504 840 # # CviJI: # [RGCY] # 8 27 41 81 123 126 # 190 200 219 329 # # CviRI*: # [TGCA] # 72 122 364 786 # # DdeI: # [CTNAG] # 40 77 99 245 257 312 # 314 # # DpnI: # [GATC] # 14 36 73 117 133 289 # 682 # # Eam1105I: # [GACNNNNNGTC] # 60 1284 # # Eco31I: # [GGTCTC] # 629 715 # # Eco57MI: # [CTGRAG] # 102 612 630 # # EcoNI: # [CCTNNNNNAGG] # 612 732 # # EcoP15I: # [CAGCAG] # 405 939 # # EcoRII: # [CCWGG] # 178 559 607 # # FatI: # [CATG] # 505 839 # # FauI: # [CCCGC] # 192 1152 # # FnuDII*: # [CGCG] # 43 68 131 1102 # # FokI: # [GGATG] # 4 196 556 588 # # GlaI: # [GCGC] # 29 42 130 280 863 # # GsuI: # [CTGGAG] # 102 612 630 # # HaeIII: # [GGCC] # 123 200 227 794 # # HgaI: # [GACGC] # 288 402 654 # # HgiAI*: # [GWGCWC] # 52 1292 # # HgiJII*: # [GRGCYC] # 43 190 234 877 # # HhaI: # [GCGC] # 29 41 131 280 863 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 0 0 # 32 32 32 32 46 124 # 274 278 494 # # Hin4II*: # [CCTTC] # 237 416 691 # # Hin6I: # [GCGC] # 29 43 129 280 863 # # HinfI: # [GANTC] # 69 105 218 952 # # HpaII: # [CCGG] # 126 155 380 683 # # HphI: # [GGTGA] # 8 98 114 469 655 # # Hpy166II: # [GTNNAC] # 58 259 267 349 411 # # Hpy178III*: # [TCNNGA] # 21 26 56 64 81 103 # 105 114 141 300 333 # # Hpy188I: # [TCNGA] # 52 54 119 142 209 768 # # Hpy99I: # [CGWCG] # 115 1229 # # Ksp632I*: # [CTCTTC] # 76 225 408 635 # # MaeI: # [CTAG] # 86 155 1103 # # MaeIII: # [GTNAC] # 20 80 132 205 236 310 # 361 # # MboI: # [GATC] # 14 34 73 117 133 291 # 682 # # MboII: # [GAAGA] # 6 59 169 212 247 269 # 382 # # McrI*: # [CGRYCG] # 37 435 872 # # MfeI: # [CAATTG] # 373 971 # # MlyI: # [GAGTC] # 224 1120 # # MmeI: # [TCCRAC] # 77 1267 # # MnlI: # [CCTC] # 12 27 48 75 83 94 # 109 114 178 272 332 # # MseI: # [TTAA] # 8 9 10 77 115 141 # 172 812 # # MslI: # [CAYNNNNRTG] # 436 908 # # MstI*: # [TGCGCA] # 410 934 # # MwoI: # [GCNNNNNNNGC] # 9 47 144 1144 # # NlaIII: # [CATG] # 501 843 # # NlaIV: # [GGNNCC] # 207 1137 # # OliI: # [CACNNNNGTG] # 436 908 # # PasI: # [CCCWGGG] # 177 1167 # # PleI: # [GAGTC] # 224 1120 # # PvuI: # [CGATCG] # 37 435 872 # # RsaI: # [GTAC] # 34 227 498 585 # # SapI: # [GCTCTTC] # 225 1119 # # ScrFI: # [CCNGG] # 176 559 609 # # SduI: # [GDGCHC] # 9 43 181 234 877 # # SecI*: # [CCNNGG] # 1 177 559 607 # # SetI: # [ASST] # 26 27 50 52 75 96 # 140 275 603 # # SfaNI: # [GCATC] # 96 324 924 # # SmlI: # [CTYRAG] # 246 1098 # # StuI: # [AGGCCT] # 550 794 # # TaqI: # [TCGA] # 9 33 425 877 # # TaqII: # [CACCCA] # 387 957 # # TatI: # [WGTACW] # 583 761 # # TauI: # [GCSGC] # 427 917 # # TfiI: # [GAWTC] # 69 323 952 # # TseI: # [GCWGC] # 435 909 # # TsoI: # [TARCCA] # 150 1194 # # Tsp45I: # [GTSAC] # 20 80 236 1008 # # Tsp4CI*: # [ACNGT] # 17 62 100 151 201 813 # # TspDTI: # [ATGAA] # 131 218 262 317 416 # # TspEI: # [AATT] # 13 30 30 56 77 103 # 129 137 149 161 182 277 # # TspRI: # [CASTGNN] # 78 1266 # # XhoII: # [RGATCY] # 151 1193 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1344 # Reported_sequences: 1 # Reported_hitcount: 291 #---------------------------------------