Restriction Fragments for BDS1/YOL164W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

BDS1/YOL164W on chromosome XV from coordinates 6175 to 8115.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.8390.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 7 12 + NlaIV GGNNCC 9 9 . . 7 12 + HgiCI* GGYRCC 7 11 . . 11 21 + EcoNI CCTNNNNNAGG 15 16 . . 11 21 + BsiYI* CCNNNNNNNGG 17 14 . . 14 17 + MseI TTAA 14 16 . . 20 25 + NlaIV GGNNCC 22 22 . . 18 21 - MnlI CCTC 11 10 . . 23 27 + Tsp4CI* ACNGT 25 24 . . 24 29 + DsaI* CCRYGG 24 28 . . 24 29 + SecI* CCNNGG 24 28 . . 35 39 + DdeI CTNAG 35 38 . . 35 39 + BspCNI CTCAG 48 46 . . 35 39 + BseMII CTCAG 49 47 . . 48 52 - Hin4II* CCTTC 42 41 . . 56 61 + Hpy166II GTNNAC 58 58 . . 56 61 + HindII GTYRAC 58 58 . . 60 63 + SetI ASST 63 59 . . 61 65 + Hin4II* CCTTC 71 70 . . 67 70 + TaiI ACGT 70 66 . . 67 70 + SetI ASST 70 66 . . 67 70 + MaeII ACGT 67 69 . . 70 73 + MseI TTAA 70 72 . . 74 84 + FalI AAGNNNNNCTT 65 60 97 92 75 78 + CviJI RGCY 76 76 . . 77 91 + XcmI CCANNNNNNNNNTGG 84 83 . . 80 83 + SetI ASST 83 79 . . 81 91 + BsiYI* CCNNNNNNNGG 87 84 . . 74 84 - FalI AAGNNNNNCTT 97 92 65 60 108 112 + DdeI CTNAG 108 111 . . 108 112 + BspCNI CTCAG 121 119 . . 108 112 + BseMII CTCAG 122 120 . . 109 113 + Hpy188I TCNGA 111 110 . . 112 115 + DpnI GATC 113 113 . . 112 115 + BstKTI GATC 114 112 . . 112 115 + MboI GATC 111 115 . . 115 119 + Hpy99I CGWCG 119 114 . . 117 122 + Hpy178III* TCNNGA 118 120 . . 117 122 + NruI TCGCGA 119 119 . . 118 121 + FnuDII* CGCG 119 119 . . 125 128 + TaqI TCGA 125 127 . . 129 133 + FokI GGATG 142 146 . . 129 133 + BseGI GGATG 135 133 . . 127 130 - MnlI CCTC 120 119 . . 130 134 - SfaNI GCATC 120 124 . . 137 142 + SmlI CTYRAG 137 141 . . 141 144 + SetI ASST 144 140 . . 137 142 - Bce83I* CTTGAG 122 120 . . 143 147 + MaeIII GTNAC 142 147 . . 146 156 + BsaXI ACNNNNNCTCC 136 133 166 163 151 154 + CviJI RGCY 152 152 . . 151 156 + NlaIV GGNNCC 153 153 . . 160 164 + Hpy188I TCNGA 162 161 . . 178 181 + CviJI RGCY 179 179 . . 178 181 + HaeIII GGCC 179 179 . . 178 182 + BmgT120I GGNCC 179 180 . . 178 182 + AsuI* GGNCC 178 181 . . 179 189 + MwoI GCNNNNNNNGC 185 182 . . 179 189 + BglI GCCNNNNNGGC 185 182 . . 181 191 + BsiYI* CCNNNNNNNGG 187 184 . . 185 189 + BceAI ACGGC 201 203 . . 188 191 - AciI CCGC 188 190 . . 205 209 + TspDTI ATGAA 220 218 . . 217 220 + CviJI RGCY 218 218 . . 225 230 + NlaIV GGNNCC 227 227 . . 227 230 + CviJI RGCY 228 228 . . 228 238 + MwoI GCNNNNNNNGC 234 231 . . 230 234 + DdeI CTNAG 230 233 . . 234 238 + HgaI GACGC 243 248 . . 230 234 - BspCNI CTCAG 222 220 . . 230 234 - BseMII CTCAG 221 219 . . 234 239 + AcyI GRCGYC 235 237 . . 232 236 - BsmAI GTCTC 226 230 . . 237 242 + Cac8I GCNNGC 239 239 . . 232 237 - Esp3I CGTCTC 226 230 . . 238 242 + FauI CCCGC 246 248 . . 239 242 + AciI CCGC 239 241 . . 247 251 + Tsp4CI* ACNGT 249 248 . . 250 255 + Hpy166II GTNNAC 252 252 . . 250 255 + HpaI GTTAAC 252 252 . . 250 255 + HindII GTYRAC 252 252 . . 251 254 + MseI TTAA 251 253 . . 257 260 + MaeI CTAG 257 259 . . 259 262 + SetI ASST 262 258 . . 259 262 + CviJI RGCY 260 260 . . 259 262 + AluI AGCT 260 260 . . 267 271 - BsmAI GTCTC 261 265 . . 274 277 + Hin6I GCGC 274 276 . . 274 277 + GlaI GCGC 275 275 . . 274 277 + HhaI GCGC 276 274 . . 276 280 + BlsI GCNGC 278 277 . . 276 280 + BisI GCNGC 277 278 . . 276 280 + BbvI GCAGC 288 292 . . 276 280 + TseI GCWGC 276 279 . . 276 287 + AlfI GCANNNNNNTGC 265 263 299 297 277 282 + PvuII CAGCTG 279 279 . . 277 282 + NspBII* CMGCKG 279 279 . . 278 281 + SetI ASST 281 277 . . 278 281 + CviJI RGCY 279 279 . . 278 281 + AluI AGCT 279 279 . . 282 287 + BsmI GAATGC 288 286 . . 276 287 - AlfI GCANNNNNNTGC 299 297 265 263 292 295 + FatI CATG 291 295 . . 292 295 + NlaIII CATG 295 291 . . 292 295 + CviAII CATG 292 294 . . 290 294 - TspDTI ATGAA 280 278 . . 295 298 + CviJI RGCY 296 296 . . 295 298 + HaeIII GGCC 296 296 . . 301 306 + AhaIII* TTTAAA 303 303 . . 302 305 + MseI TTAA 302 304 . . 309 315 + TspRI CASTGNN 315 306 . . 316 321 + AclI AACGTT 317 319 . . 317 320 + TaiI ACGT 320 316 . . 317 320 + SetI ASST 320 316 . . 317 320 + MaeII ACGT 317 319 . . 319 324 + Hpy166II GTNNAC 321 321 . . 323 329 + SexAI ACCWGGT 323 328 . . 324 328 + BssKI CCNGG 323 328 . . 324 328 + BseBI CCWGG 325 326 . . 324 328 + ScrFI CCNGG 325 326 . . 324 328 + EcoRII CCWGG 323 328 . . 326 329 + SetI ASST 329 325 . . 332 335 - MnlI CCTC 325 324 . . 335 345 + Hin4I GAYNNNNNVTC 326 321 358 353 337 340 + MaeI CTAG 337 339 . . 340 345 + EcoRV GATATC 342 342 . . 335 345 - Hin4I GAYNNNNNVTC 358 353 326 321 362 365 + TaqI TCGA 362 364 . . 366 369 + SetI ASST 369 365 . . 364 368 - Hin4II* CCTTC 358 357 . . 407 415 + AlwNI CAGNNNCTG 412 409 . . 409 413 - BsmAI GTCTC 403 407 . . 419 429 + FalI AAGNNNNNCTT 410 405 442 437 421 425 + PleI GAGTC 429 430 . . 421 425 + MlyI GAGTC 430 430 . . 421 425 + HinfI GANTC 421 424 . . 428 431 + MseI TTAA 428 430 . . 419 429 - FalI AAGNNNNNCTT 442 437 410 405 438 443 + SfeI* CTRYAG 438 442 . . 443 451 + Tth111I GACNNNGTC 446 447 . . 451 454 + MnlI CCTC 461 460 . . 452 456 + DdeI CTNAG 452 455 . . 452 456 + BspCNI CTCAG 465 463 . . 452 456 + BseMII CTCAG 466 464 . . 453 457 + Hpy188I TCNGA 455 454 . . 459 462 + SetI ASST 462 458 . . 467 470 + Csp6I GTAC 467 469 . . 467 470 + RsaI GTAC 468 468 . . 469 473 + Tsp4CI* ACNGT 471 470 . . 486 491 + TsoI TARCCA 502 500 . . 487 490 + CviJI RGCY 488 488 . . 493 497 + Hpy188I TCNGA 495 494 . . 495 499 + BmgT120I GGNCC 496 497 . . 495 499 + AvaII GGWCC 495 498 . . 495 499 + AsuI* GGNCC 495 498 . . 506 511 + BseRI GAGGAG 521 519 . . 506 509 - MnlI CCTC 499 498 . . 512 515 + MseI TTAA 512 514 . . 519 522 + TspEI AATT 518 522 . . 535 545 + Hin4I GAYNNNNNVTC 526 521 558 553 544 549 + Hpy178III* TCNNGA 545 547 . . 544 549 + BetI* WCCGGW 544 548 . . 544 549 + BspMII* TCCGGA 544 548 . . 535 545 - Hin4I GAYNNNNNVTC 558 553 526 521 545 548 + HpaII CCGG 545 547 . . 551 554 + SetI ASST 554 550 . . 550 553 - MnlI CCTC 543 542 . . 554 557 + CviRI* TGCA 555 555 . . 564 567 + CviJI RGCY 565 565 . . 568 572 - BsrI ACTGG 568 566 . . 567 572 - BfiI ACTGGG 562 561 . . 574 577 + CviJI RGCY 575 575 . . 579 582 + FatI CATG 578 582 . . 579 582 + NlaIII CATG 582 578 . . 579 582 + CviAII CATG 579 581 . . 577 581 - TspDTI ATGAA 567 565 . . 598 607 + XmnI GAANNNNTTC 602 602 . . 610 620 + MwoI GCNNNNNNNGC 616 613 . . 610 620 + BstAPI GCANNNNNTGC 616 613 . . 615 620 + BsmI GAATGC 621 619 . . 619 624 + BtgZI GCGATG 634 638 . . 622 627 + EcoT22I ATGCAT 626 622 . . 623 626 + CviRI* TGCA 624 624 . . 624 628 + SfaNI GCATC 633 637 . . 621 625 - SfaNI GCATC 611 615 . . 627 630 + TaqI TCGA 627 629 . . 628 632 + Hpy99I CGWCG 632 627 . . 630 633 + TaiI ACGT 633 629 . . 630 633 + SetI ASST 633 629 . . 630 633 + MaeII ACGT 630 632 . . 636 650 + XcmI CCANNNNNNNNNTGG 643 642 . . 654 658 + MaeIII GTNAC 653 658 . . 666 672 + TspRI CASTGNN 672 663 . . 665 669 - BsrI ACTGG 665 663 . . 664 669 - BfiI ACTGGG 659 658 . . 671 674 + MseI TTAA 671 673 . . 679 682 + FatI CATG 678 682 . . 679 682 + NlaIII CATG 682 678 . . 679 682 + CviAII CATG 679 681 . . 702 705 + SetI ASST 705 701 . . 702 705 + CviJI RGCY 703 703 . . 702 705 + AluI AGCT 703 703 . . 703 707 + BlsI GCNGC 705 704 . . 703 707 + BisI GCNGC 704 705 . . 703 707 + TseI GCWGC 703 706 . . 707 710 + MnlI CCTC 717 716 . . 703 707 - BbvI GCAGC 690 694 . . 713 717 + TspGWI ACGGA 728 726 . . 718 722 + Tsp4CI* ACNGT 720 719 . . 721 725 + MaeIII GTNAC 720 725 . . 725 731 + TspRI CASTGNN 731 722 . . 732 735 + CviRI* TGCA 733 733 . . 751 755 + MboII GAAGA 763 762 . . 754 759 + SduI GDGCHC 758 754 . . 754 759 + HgiJII* GRGCYC 758 754 . . 755 758 + CviJI RGCY 756 756 . . 751 756 - Ksp632I* CTCTTC 746 749 . . 751 757 - SapI GCTCTTC 746 749 . . 759 763 + Hpy99I CGWCG 763 758 . . 761 764 + TaqI TCGA 761 763 . . 763 767 + MboII GAAGA 775 774 . . 768 772 + TspGWI ACGGA 783 781 . . 773 778 + MfeI CAATTG 773 777 . . 774 777 + TspEI AATT 773 777 . . 778 782 - BccI CCATC 772 773 . . 800 803 + SetI ASST 803 799 . . 800 803 + CviJI RGCY 801 801 . . 800 803 + AluI AGCT 801 801 . . 801 806 + Cac8I GCNNGC 803 803 . . 804 807 + CviRI* TGCA 805 805 . . 807 810 + SetI ASST 810 806 . . 808 812 + BssKI CCNGG 807 812 . . 808 812 + BseBI CCWGG 809 810 . . 808 812 + ScrFI CCNGG 809 810 . . 808 812 + EcoRII CCWGG 807 812 . . 811 815 + BinI* GGATC 819 820 . . 812 815 + DpnI GATC 813 813 . . 812 815 + BstKTI GATC 814 812 . . 812 815 + MboI GATC 811 815 . . 814 818 + Hpy188I TCNGA 816 815 . . 805 816 - BcgI CGANNNNNNTGC 828 826 794 792 819 824 + HaeII RGCGCY 823 819 . . 820 823 + Hin6I GCGC 820 822 . . 820 823 + GlaI GCGC 821 821 . . 820 823 + HhaI GCGC 822 820 . . 823 827 + Hin4II* CCTTC 833 832 . . 826 830 + Hpy188I TCNGA 828 827 . . 837 846 + MslI CAYNNNNRTG 841 841 . . 846 856 + MwoI GCNNNNNNNGC 852 849 . . 859 864 + SduI GDGCHC 863 859 . . 859 864 + HgiAI* GWGCWC 863 859 . . 867 870 + FatI CATG 866 870 . . 867 870 + NlaIII CATG 870 866 . . 867 870 + CviAII CATG 867 869 . . 874 878 + MboII GAAGA 886 885 . . 880 884 + Tsp45I GTSAC 879 884 . . 880 884 + MaeIII GTNAC 879 884 . . 889 894 + NdeI CATATG 890 892 . . 892 897 + EcoT22I ATGCAT 896 892 . . 893 896 + CviRI* TGCA 894 894 . . 907 913 + SapI GCTCTTC 914 917 . . 908 913 + Ksp632I* CTCTTC 914 917 . . 912 915 + TaqI TCGA 912 914 . . 909 913 - MboII GAAGA 901 900 . . 915 918 + SetI ASST 918 914 . . 914 917 - MnlI CCTC 907 906 . . 925 928 + Csp6I GTAC 925 927 . . 925 928 + RsaI GTAC 926 926 . . 928 931 + FnuDII* CGCG 929 929 . . 930 934 + Hpy99I CGWCG 934 929 . . 932 936 + BceAI ACGGC 948 950 . . 945 948 + CviJI RGCY 946 946 . . 955 960 + ClaI ATCGAT 956 958 . . 956 959 + TaqI TCGA 956 958 . . 960 963 + CviRI* TGCA 961 961 . . 961 965 + BlsI GCNGC 963 962 . . 961 965 + BisI GCNGC 962 963 . . 961 965 + BbvI GCAGC 973 977 . . 961 965 + TseI GCWGC 961 964 . . 958 962 - SfaNI GCATC 948 952 . . 963 966 + SetI ASST 966 962 . . 963 966 + CviJI RGCY 964 964 . . 963 966 + AluI AGCT 964 964 . . 964 971 + PspXI VCTCGAGB 965 969 . . 964 974 + MwoI GCNNNNNNNGC 970 967 . . 965 970 + SmlI CTYRAG 965 969 . . 965 970 + XhoI CTCGAG 965 969 . . 965 970 + BmeT110I CYCGRG 966 968 . . 965 970 + AvaI CYCGRG 965 969 . . 966 969 + TaqI TCGA 966 968 . . 988 992 + TspGWI ACGGA 1003 1001 . . 992 995 + TaiI ACGT 995 991 . . 992 995 + SetI ASST 995 991 . . 992 995 + MaeII ACGT 992 994 . . 997 1000 + MseI TTAA 997 999 . . 999 1002 + TspEI AATT 998 1002 . . 1004 1009 + GsaI CCCAGC 1008 1004 . . 1004 1009 + BseYI CCCAGC 1004 1008 . . 1008 1012 + SfaNI GCATC 1017 1021 . . 1008 1018 + MwoI GCNNNNNNNGC 1014 1011 . . 1012 1018 + TspRI CASTGNN 1018 1009 . . 1013 1017 + BsrI ACTGG 1018 1016 . . 1016 1019 + CviJI RGCY 1017 1017 . . 1016 1019 + HaeIII GGCC 1017 1017 . . 1016 1020 + BmgT120I GGNCC 1017 1018 . . 1016 1020 + AsuI* GGNCC 1016 1019 . . 1018 1028 + BsiYI* CCNNNNNNNGG 1024 1021 . . 1019 1029 + BsiYI* CCNNNNNNNGG 1025 1022 . . 1022 1027 + SfeI* CTRYAG 1022 1026 . . 1037 1041 + BinI* GGATC 1045 1046 . . 1038 1041 + DpnI GATC 1039 1039 . . 1038 1041 + BstKTI GATC 1040 1038 . . 1038 1041 + MboI GATC 1037 1041 . . 1035 1038 - AciI CCGC 1035 1037 . . 1043 1047 + TspDTI ATGAA 1058 1056 . . 1046 1049 + TspEI AATT 1045 1049 . . 1050 1060 + MwoI GCNNNNNNNGC 1056 1053 . . 1051 1056 + Eco57MI CTGRAG 1072 1070 . . 1051 1056 + Eco57I CTGAAG 1072 1070 . . 1053 1057 + MboII GAAGA 1065 1064 . . 1059 1063 + BlsI GCNGC 1061 1060 . . 1059 1063 + BisI GCNGC 1060 1061 . . 1059 1063 + BbvI GCAGC 1071 1075 . . 1059 1063 + TseI GCWGC 1059 1062 . . 1060 1065 + NspBII* CMGCKG 1062 1062 . . 1062 1065 - AciI CCGC 1062 1064 . . 1062 1066 - FauI CCCGC 1055 1057 . . 1075 1080 + ApoI RAATTY 1075 1079 . . 1076 1079 + TspEI AATT 1075 1079 . . 1078 1082 - TspDTI ATGAA 1068 1066 . . 1083 1088 + Hpy178III* TCNNGA 1084 1086 . . 1083 1088 + BspHI TCATGA 1083 1087 . . 1084 1087 + FatI CATG 1083 1087 . . 1084 1087 + NlaIII CATG 1087 1083 . . 1084 1087 + CviAII CATG 1084 1086 . . 1082 1086 - TspDTI ATGAA 1072 1070 . . 1098 1101 + TaqI TCGA 1098 1100 . . 1106 1109 + MseI TTAA 1106 1108 . . 1121 1125 - BsrI ACTGG 1121 1119 . . 1129 1134 + EcoRV GATATC 1131 1131 . . 1138 1147 + XmnI GAANNNNTTC 1142 1142 . . 1142 1147 + Ksp632I* CTCTTC 1148 1151 . . 1143 1147 - MboII GAAGA 1135 1134 . . 1153 1157 + BccI CCATC 1161 1162 . . 1154 1158 - FokI GGATG 1140 1144 . . 1154 1158 - BseGI GGATG 1153 1151 . . 1161 1166 + Hpy178III* TCNNGA 1162 1164 . . 1157 1161 - BsrI ACTGG 1157 1155 . . 1162 1167 + SmlI CTYRAG 1162 1166 . . 1162 1167 + Bce83I* CTTGAG 1183 1181 . . 1176 1181 + Hpy166II GTNNAC 1178 1178 . . 1176 1181 + HindII GTYRAC 1178 1178 . . 1181 1186 + BsiI* CACGAG 1181 1185 . . 1185 1188 + SetI ASST 1188 1184 . . 1184 1187 - MnlI CCTC 1177 1176 . . 1187 1191 + MaeIII GTNAC 1186 1191 . . 1193 1197 + BceAI ACGGC 1209 1211 . . 1203 1207 + PleI GAGTC 1211 1212 . . 1203 1207 + MlyI GAGTC 1212 1212 . . 1203 1207 + HinfI GANTC 1203 1206 . . 1205 1209 + Tsp45I GTSAC 1204 1209 . . 1205 1209 + MaeIII GTNAC 1204 1209 . . 1218 1221 + CviJI RGCY 1219 1219 . . 1235 1238 + SetI ASST 1238 1234 . . 1236 1240 + BssKI CCNGG 1235 1240 . . 1236 1240 + BseBI CCWGG 1237 1238 . . 1236 1241 + SecI* CCNNGG 1236 1240 . . 1236 1240 + ScrFI CCNGG 1237 1238 . . 1236 1240 + EcoRII CCWGG 1235 1240 . . 1249 1258 + BsaBI GATNNNNATC 1253 1253 . . 1251 1254 + CviRI* TGCA 1252 1252 . . 1252 1262 + MwoI GCNNNNNNNGC 1258 1255 . . 1249 1253 - SfaNI GCATC 1239 1243 . . 1258 1261 + HpaII CCGG 1258 1260 . . 1260 1263 + CviJI RGCY 1261 1261 . . 1265 1268 + SetI ASST 1268 1264 . . 1287 1290 + CviJI RGCY 1288 1288 . . 1293 1296 + CviRI* TGCA 1294 1294 . . 1294 1304 + MwoI GCNNNNNNNGC 1300 1297 . . 1307 1310 + TaqI TCGA 1307 1309 . . 1319 1323 + TauI GCSGC 1322 1319 . . 1319 1323 + BlsI GCNGC 1321 1320 . . 1319 1323 + BisI GCNGC 1320 1321 . . 1321 1326 + HaeII RGCGCY 1325 1321 . . 1322 1325 + Hin6I GCGC 1322 1324 . . 1322 1325 + GlaI GCGC 1323 1323 . . 1322 1325 + HhaI GCGC 1324 1322 . . 1319 1322 - AciI CCGC 1319 1321 . . 1330 1340 + MwoI GCNNNNNNNGC 1336 1333 . . 1327 1331 - SfaNI GCATC 1317 1321 . . 1344 1347 + CviJI RGCY 1345 1345 . . 1347 1352 + PsiI TTATAA 1349 1349 . . 1358 1363 + Hpy178III* TCNNGA 1359 1361 . . 1383 1386 + CviRI* TGCA 1384 1384 . . 1384 1389 + Cac8I GCNNGC 1386 1386 . . 1394 1397 + MseI TTAA 1394 1396 . . 1399 1404 + MfeI CAATTG 1399 1403 . . 1400 1403 + TspEI AATT 1399 1403 . . 1422 1427 + TsoI TARCCA 1438 1436 . . 1425 1429 + BccI CCATC 1433 1434 . . 1431 1434 + SetI ASST 1434 1430 . . 1431 1434 + CviJI RGCY 1432 1432 . . 1431 1434 + AluI AGCT 1432 1432 . . 1434 1439 + Hpy178III* TCNNGA 1435 1437 . . 1438 1443 + SacI GAGCTC 1442 1438 . . 1438 1443 + Ecl136II GAGCTC 1440 1440 . . 1438 1443 + SduI GDGCHC 1442 1438 . . 1433 1438 - BsiI* CACGAG 1433 1437 . . 1438 1443 + HgiJII* GRGCYC 1442 1438 . . 1438 1443 + HgiAI* GWGCWC 1442 1438 . . 1439 1442 + SetI ASST 1442 1438 . . 1439 1442 + CviJI RGCY 1440 1440 . . 1439 1442 + AluI AGCT 1440 1440 . . 1450 1454 + HgaI GACGC 1459 1464 . . 1462 1467 + PvuII CAGCTG 1464 1464 . . 1462 1467 + NspBII* CMGCKG 1464 1464 . . 1463 1466 + SetI ASST 1466 1462 . . 1463 1466 + CviJI RGCY 1464 1464 . . 1463 1466 + AluI AGCT 1464 1464 . . 1464 1469 - GsaI CCCAGC 1468 1464 . . 1464 1469 - BseYI CCCAGC 1464 1468 . . 1482 1485 + CviJI RGCY 1483 1483 . . 1483 1488 + Cac8I GCNNGC 1485 1485 . . 1484 1494 + BsiYI* CCNNNNNNNGG 1490 1487 . . 1484 1494 + PflMI CCANNNNNTGG 1490 1487 . . 1487 1497 + MwoI GCNNNNNNNGC 1493 1490 . . 1488 1493 + BsaAI YACGTR 1490 1490 . . 1488 1493 + PmaCI CACGTG 1490 1490 . . 1489 1492 + TaiI ACGT 1492 1488 . . 1489 1492 + SetI ASST 1492 1488 . . 1489 1492 + MaeII ACGT 1489 1491 . . 1494 1497 + Hin6I GCGC 1494 1496 . . 1494 1497 + GlaI GCGC 1495 1495 . . 1494 1497 + HhaI GCGC 1496 1494 . . 1505 1508 + SetI ASST 1508 1504 . . 1513 1518 + NarI GGCGCC 1514 1516 . . 1513 1518 + KasI GGCGCC 1513 1517 . . 1513 1518 + DinI GGCGCC 1515 1515 . . 1513 1518 + HaeII RGCGCY 1517 1513 . . 1513 1518 + NlaIV GGNNCC 1515 1515 . . 1513 1518 + AcyI GRCGYC 1514 1516 . . 1513 1518 + HgiCI* GGYRCC 1513 1517 . . 1514 1517 + Hin6I GCGC 1514 1516 . . 1514 1517 + GlaI GCGC 1515 1515 . . 1514 1517 + HhaI GCGC 1516 1514 . . 1517 1522 + DsaI* CCRYGG 1517 1521 . . 1517 1522 + StyI CCWWGG 1517 1521 . . 1517 1522 + NcoI CCATGG 1517 1521 . . 1517 1522 + SecI* CCNNGG 1517 1521 . . 1518 1521 + FatI CATG 1517 1521 . . 1518 1521 + NlaIII CATG 1521 1517 . . 1518 1521 + CviAII CATG 1518 1520 . . 1527 1530 + Hin6I GCGC 1527 1529 . . 1527 1530 + GlaI GCGC 1528 1528 . . 1527 1530 + HhaI GCGC 1529 1527 . . 1530 1535 + StyI CCWWGG 1530 1534 . . 1530 1535 + SecI* CCNNGG 1530 1534 . . 1533 1536 + SetI ASST 1536 1532 . . 1535 1540 + Hpy166II GTNNAC 1537 1537 . . 1537 1540 + Csp6I GTAC 1537 1539 . . 1537 1540 + RsaI GTAC 1538 1538 . . 1552 1557 + TatI WGTACW 1552 1556 . . 1552 1557 + ScaI AGTACT 1554 1554 . . 1553 1556 + Csp6I GTAC 1553 1555 . . 1553 1556 + RsaI GTAC 1554 1554 . . 1555 1559 + BsrI ACTGG 1560 1558 . . 1563 1566 + Hin6I GCGC 1563 1565 . . 1563 1566 + GlaI GCGC 1564 1564 . . 1563 1566 + HhaI GCGC 1565 1563 . . 1570 1575 + SspI AATATT 1572 1572 . . 1576 1580 - BceAI ACGGC 1561 1563 . . 1627 1637 + MwoI GCNNNNNNNGC 1633 1630 . . 1641 1644 + SetI ASST 1644 1640 . . 1639 1643 - Hin4II* CCTTC 1633 1632 . . 1647 1650 + TspEI AATT 1646 1650 . . 1660 1663 + TspEI AATT 1659 1663 . . 1672 1677 + VspI ATTAAT 1673 1675 . . 1669 1673 - TspDTI ATGAA 1659 1657 . . 1673 1676 + MseI TTAA 1673 1675 . . 1675 1678 + TspEI AATT 1674 1678 . . 1682 1686 + TspDTI ATGAA 1697 1695 . . 1686 1690 + Tsp4CI* ACNGT 1688 1687 . . 1691 1696 + Hpy178III* TCNNGA 1692 1694 . . 1694 1699 + BclI TGATCA 1694 1698 . . 1695 1698 + DpnI GATC 1696 1696 . . 1695 1698 + BstKTI GATC 1697 1695 . . 1695 1698 + MboI GATC 1694 1698 . . 1701 1706 + Hpy178III* TCNNGA 1702 1704 . . 1703 1706 + TaqI TCGA 1703 1705 . . 1704 1715 + BcgI CGANNNNNNTGC 1693 1691 1727 1725 1709 1713 + Tsp4CI* ACNGT 1711 1710 . . 1712 1717 + SduI GDGCHC 1716 1712 . . 1712 1717 + BseSI GKGCMC 1716 1712 . . 1720 1723 + SetI ASST 1723 1719 . . 1732 1735 + CviJI RGCY 1733 1733 . . 1733 1738 + Cac8I GCNNGC 1735 1735 . . 1737 1748 + AlfI GCANNNNNNTGC 1726 1724 1760 1758 1746 1749 + CviRI* TGCA 1747 1747 . . 1744 1748 - SfaNI GCATC 1734 1738 . . 1737 1748 - AlfI GCANNNNNNTGC 1760 1758 1726 1724 1752 1755 + CviRI* TGCA 1753 1753 . . 1750 1754 - SfaNI GCATC 1740 1744 . . 1758 1761 + TspEI AATT 1757 1761 . . 1784 1789 + HindIII AAGCTT 1784 1788 . . 1785 1788 + SetI ASST 1788 1784 . . 1785 1788 + CviJI RGCY 1786 1786 . . 1785 1788 + AluI AGCT 1786 1786 . . 1786 1796 + MwoI GCNNNNNNNGC 1792 1789 . . 1793 1798 + BalI TGGCCA 1795 1795 . . 1793 1798 + CfrI YGGCCR 1793 1797 . . 1794 1797 + CviJI RGCY 1795 1795 . . 1794 1797 + HaeIII GGCC 1795 1795 . . 1799 1803 + TspDTI ATGAA 1814 1812 . . 1803 1806 + TspEI AATT 1802 1806 . . 1822 1825 + MseI TTAA 1822 1824 . . 1826 1829 + DpnI GATC 1827 1827 . . 1826 1829 + BstKTI GATC 1828 1826 . . 1826 1829 + MboI GATC 1825 1829 . . 1838 1841 + TaqI TCGA 1838 1840 . . 1846 1849 - MnlI CCTC 1839 1838 . . 1854 1857 + TaqI TCGA 1854 1856 . . 1856 1860 + HinfI GANTC 1856 1859 . . 1858 1862 + DdeI CTNAG 1858 1861 . . 1858 1862 + BspCNI CTCAG 1871 1869 . . 1858 1862 + BseMII CTCAG 1872 1870 . . 1859 1863 + Hpy188I TCNGA 1861 1860 . . 1856 1860 - PleI GAGTC 1850 1851 . . 1856 1860 - MlyI GAGTC 1850 1850 . . 1863 1866 + TspEI AATT 1862 1866 . . 1867 1871 + MboII GAAGA 1879 1878 . . 1871 1874 + SetI ASST 1874 1870 . . 1871 1874 + CviJI RGCY 1872 1872 . . 1871 1874 + AluI AGCT 1872 1872 . . 1867 1872 - Ksp632I* CTCTTC 1862 1865 . . 1867 1873 - SapI GCTCTTC 1862 1865 . . 1895 1898 + TaqI TCGA 1895 1897 . . 1899 1903 - BsrI ACTGG 1899 1897 . . 1911 1915 + Hpy99I CGWCG 1915 1910 . . 1919 1922 + CviJI RGCY 1920 1920 . . 1921 1931 + BsiYI* CCNNNNNNNGG 1927 1924 . . #--------------------------------------- # # # AciI: # [CCGC] # 27 51 188 257 622 796 # # AclI: # [AACGTT] # 317 1624 # # AcyI: # [GRCGYC] # 235 427 1279 # # AhaIII*: # [TTTAAA] # 303 1638 # # AlfI: # [GCANNNNNNTGC] # 0 0 0 0 34 34 # 181 265 1427 # # AluI: # [AGCT] # 8 19 24 69 86 98 # 163 260 322 424 468 # # AlwNI: # [CAGNNNCTG] # 412 1529 # # ApoI: # [RAATTY] # 866 1075 # # AsuI*: # [GGNCC] # 178 317 521 925 # # AvaI: # [CYCGRG] # 965 976 # # AvaII: # [GGWCC] # 495 1446 # # BalI: # [TGGCCA] # 146 1795 # # BbvI: # [GCAGC] # 98 283 288 402 870 # # BccI: # [CCATC] # 272 389 508 772 # # Bce83I*: # [CTTGAG] # 122 758 1061 # # BceAI: # [ACGGC] # 201 261 352 380 747 # # BcgI: # [CGANNNNNNTGC] # 34 34 214 794 865 # # BclI: # [TGATCA] # 247 1694 # # BetI*: # [WCCGGW] # 544 1397 # # BfiI: # [ACTGGG] # 97 562 1282 # # BglI: # [GCCNNNNNGGC] # 185 1756 # # BinI*: # [GGATC] # 226 819 896 # # BisI: # [GCNGC] # 98 258 260 277 427 621 # # BlsI: # [GCNGC] # 98 258 260 278 427 620 # # BmeT110I: # [CYCGRG] # 966 975 # # BmgT120I: # [GGNCC] # 179 317 521 924 # # BsaAI: # [YACGTR] # 451 1490 # # BsaBI: # [GATNNNNATC] # 688 1253 # # BsaXI: # [ACNNNNNCTCC] # 30 136 1775 # # BseBI: # [CCWGG] # 325 428 484 704 # # BseGI: # [GGATG] # 135 788 1018 # # BseMII: # [CTCAG] # 49 69 73 99 245 1406 # # BseRI: # [GAGGAG] # 521 1420 # # BseSI: # [GKGCMC] # 225 1716 # # BseYI: # [CCCAGC] # 460 477 1004 # # BsiI*: # [CACGAG] # 252 508 1181 # # BsiYI*: # [CCNNNNNNNGG] # 1 14 17 70 100 437 # 465 837 # # BsmAI: # [GTCTC] # 35 142 226 1538 # # BsmI: # [GAATGC] # 288 333 1320 # # BspCNI: # [CTCAG] # 48 70 73 101 243 1406 # # BspHI: # [TCATGA] # 858 1083 # # BspMII*: # [TCCGGA] # 544 1397 # # BsrI: # [ACTGG] # 36 42 97 103 339 353 # 403 568 # # BssKI: # [CCNGG] # 323 428 484 706 # # BstAPI: # [GCANNNNNTGC] # 616 1325 # # BstKTI: # [GATC] # 113 114 131 226 657 700 # # BtgZI: # [GCGATG] # 634 1307 # # Cac8I: # [GCNNGC] # 99 206 239 250 564 583 # # CfrI: # [YGGCCR] # 148 1793 # # ClaI: # [ATCGAT] # 956 985 # # Csp6I: # [GTAC] # 16 388 458 467 612 # # CviAII: # [CATG] # 100 188 217 287 292 423 # 434 # # CviJI: # [RGCY] # 8 9 10 10 17 18 # 19 19 21 24 27 27 # 32 39 42 45 48 53 # 53 53 57 76 76 77 # 77 87 128 145 192 202 # 250 # # CviRI*: # [TGCA] # 6 42 67 69 72 89 # 90 109 188 291 363 555 # # DdeI: # [CTNAG] # 35 73 83 122 222 1406 # # DinI: # [GGCGCC] # 426 1515 # # DpnI: # [GATC] # 113 114 131 226 657 700 # # DsaI*: # [CCRYGG] # 24 424 1493 # # Ecl136II: # [GAGCTC] # 501 1440 # # Eco57I: # [CTGAAG] # 869 1072 # # Eco57MI: # [CTGRAG] # 869 1072 # # EcoNI: # [CCTNNNNNAGG] # 15 1926 # # EcoRII: # [CCWGG] # 323 428 484 706 # # EcoRV: # [GATATC] # 342 789 810 # # EcoT22I: # [ATGCAT] # 270 626 1045 # # Esp3I: # [CGTCTC] # 226 1715 # # FalI: # [AAGNNNNNCTT] # 0 0 0 0 32 32 # 65 313 1499 # # FatI: # [CATG] # 100 188 217 287 291 424 # 434 # # FauI: # [CCCGC] # 246 809 886 # # FnuDII*: # [CGCG] # 119 810 1012 # # FokI: # [GGATG] # 142 801 998 # # GlaI: # [GCGC] # 13 20 36 172 275 377 # 502 546 # # GsaI: # [CCCAGC] # 460 473 1008 # # HaeII: # [RGCGCY] # 192 424 502 823 # # HaeIII: # [GGCC] # 117 146 179 721 778 # # HgaI: # [GACGC] # 243 482 1216 # # HgiAI*: # [GWGCWC] # 499 579 863 # # HgiCI*: # [GGYRCC] # 7 428 1506 # # HgiJII*: # [GRGCYC] # 499 684 758 # # HhaI: # [GCGC] # 13 20 36 172 276 376 # 502 546 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 32 32 # 168 326 1383 # # Hin4II*: # [CCTTC] # 29 42 287 308 475 800 # # Hin6I: # [GCGC] # 13 20 36 172 274 378 # 502 546 # # HindII: # [GTYRAC] # 58 194 763 926 # # HindIII: # [AAGCTT] # 157 1784 # # HinfI: # [GANTC] # 85 421 653 782 # # HpaI: # [GTTAAC] # 252 1689 # # HpaII: # [CCGG] # 545 683 713 # # Hpy166II: # [GTNNAC] # 58 69 194 359 404 857 # # Hpy178III*: # [TCNNGA] # 10 76 78 118 197 239 # 257 427 539 # # Hpy188I: # [TCNGA] # 12 40 51 80 111 293 # 321 1033 # # Hpy99I: # [CGWCG] # 26 119 131 171 513 981 # # KasI: # [GGCGCC] # 428 1513 # # Ksp632I*: # [CTCTTC] # 79 168 234 714 746 # # MaeI: # [CTAG] # 80 257 1604 # # MaeII: # [ACGT] # 67 250 313 362 452 497 # # MaeIII: # [GTNAC] # 18 67 142 159 307 511 # 737 # # MboI: # [GATC] # 111 116 131 226 657 700 # # MboII: # [GAAGA] # 12 15 62 70 111 164 # 744 763 # # MfeI: # [CAATTG] # 542 626 773 # # MlyI: # [GAGTC] # 91 430 638 782 # # MnlI: # [CCTC] # 11 38 44 102 109 136 # 174 190 205 270 662 # # MseI: # [TTAA] # 14 51 56 84 109 119 # 126 149 159 181 279 288 # 326 # # MslI: # [CAYNNNNRTG] # 841 1100 # # MwoI: # [GCNNNNNNNGC] # 36 42 42 44 49 118 # 140 149 157 159 185 202 # 236 382 # # NarI: # [GGCGCC] # 427 1514 # # NcoI: # [CCATGG] # 424 1517 # # NdeI: # [CATATG] # 890 1051 # # NlaIII: # [CATG] # 100 188 217 287 295 420 # 434 # # NlaIV: # [GGNNCC] # 9 13 74 131 426 1288 # # NruI: # [TCGCGA] # 119 1822 # # NspBII*: # [CMGCKG] # 279 402 477 783 # # PflMI: # [CCANNNNNTGG] # 451 1490 # # PleI: # [GAGTC] # 91 429 639 782 # # PmaCI: # [CACGTG] # 451 1490 # # PsiI: # [TTATAA] # 592 1349 # # PspXI: # [VCTCGAGB] # 965 976 # # PvuII: # [CAGCTG] # 279 477 1185 # # RsaI: # [GTAC] # 16 387 458 468 612 # # SacI: # [GAGCTC] # 499 1442 # # SapI: # [GCTCTTC] # 79 168 746 948 # # ScaI: # [AGTACT] # 387 1554 # # ScrFI: # [CCNGG] # 325 428 484 704 # # SduI: # [GDGCHC] # 105 225 274 579 758 # # SecI*: # [CCNNGG] # 13 24 281 411 1212 # # SetI: # [ASST] # 7 7 8 9 13 16 # 19 24 26 28 29 30 # 39 40 48 50 61 63 # 65 67 72 79 79 86 # 92 93 98 108 108 118 # 166 193 # # SexAI: # [ACCWGGT] # 323 1618 # # SfaNI: # [GCATC] # 6 22 69 78 120 201 # 222 315 417 491 # # SfeI*: # [CTRYAG] # 438 584 919 # # SmlI: # [CTYRAG] # 137 197 779 828 # # SspI: # [AATATT] # 369 1572 # # StyI: # [CCWWGG] # 13 411 1517 # # TaiI: # [ACGT] # 70 250 313 362 449 497 # # TaqI: # [TCGA] # 10 16 41 44 46 125 # 132 134 135 151 209 237 # 265 396 # # TatI: # [WGTACW] # 389 1552 # # TauI: # [GCSGC] # 619 1322 # # TseI: # [GCWGC] # 98 258 276 427 882 # # TsoI: # [TARCCA] # 502 503 936 # # Tsp45I: # [GTSAC] # 325 737 879 # # Tsp4CI*: # [ACNGT] # 23 25 222 224 230 249 # 968 # # TspDTI: # [ATGAA] # 4 10 38 60 117 127 # 220 287 491 587 # # TspEI: # [AATT] # 13 15 30 45 47 60 # 79 83 225 247 255 324 # 518 # # TspGWI: # [ACGGA] # 55 220 728 938 # # TspRI: # [CASTGNN] # 59 287 315 357 923 # # Tth111I: # [GACNNNGTC] # 446 1495 # # VspI: # [ATTAAT] # 268 1673 # # XcmI: # [CCANNNNNNNNNTGG] # 84 559 1298 # # XhoI: # [CTCGAG] # 965 976 # # XmnI: # [GAANNNNTTC] # 540 602 799 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1941 # Reported_sequences: 1 # Reported_hitcount: 539 #---------------------------------------