Restriction Fragments for ZPS1/YOL154W



Send questions or suggestions to SGD

BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

ZPS1/YOL154W on chromosome XV from coordinates 34658 to 35407.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.25827.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 1 5 + TspDTI ATGAA 16 14 . . 9 14 + Ksp632I* CTCTTC 15 18 . . 10 14 - MboII GAAGA 2 1 . . 14 17 + HpaII CCGG 14 16 . . 33 36 + CviRI* TGCA 34 34 . . 34 44 + MwoI GCNNNNNNNGC 40 37 . . 34 44 + BstAPI GCANNNNNTGC 40 37 . . 43 53 + MwoI GCNNNNNNNGC 49 46 . . 49 52 + MaeI CTAG 49 51 . . 51 54 + SetI ASST 54 50 . . 51 54 + CviJI RGCY 52 52 . . 51 54 + AluI AGCT 52 52 . . 52 62 + MwoI GCNNNNNNNGC 58 55 . . 59 64 + SduI GDGCHC 63 59 . . 59 64 + HgiAI* GWGCWC 63 59 . . 67 71 + Tsp45I GTSAC 66 71 . . 67 71 + MaeIII GTNAC 66 71 . . 76 86 + Hin4I GAYNNNNNVTC 67 62 99 94 92 96 + DdeI CTNAG 92 95 . . 89 94 - EcoP15I CAGCAG 61 63 . . 96 100 + MaeIII GTNAC 95 100 . . 92 96 - BspCNI CTCAG 84 82 . . 92 96 - BseMII CTCAG 83 81 . . 94 104 - Hin4I GAYNNNNNVTC 117 112 85 80 106 110 + Hin4II* CCTTC 116 115 . . 108 112 - TspDTI ATGAA 98 96 . . 113 118 + SmlI CTYRAG 113 117 . . 114 119 + Hpy178III* TCNNGA 115 117 . . 113 118 - Bce83I* CTTGAG 98 96 . . 119 124 + ApoI RAATTY 119 123 . . 120 123 + TspEI AATT 119 123 . . 132 136 + PleI GAGTC 140 141 . . 132 136 + MlyI GAGTC 141 141 . . 132 136 + HinfI GANTC 132 135 . . 128 133 - MmeI TCCRAC 109 107 . . 134 138 + Tsp45I GTSAC 133 138 . . 134 138 + MaeIII GTNAC 133 138 . . 163 166 + SetI ASST 166 162 . . 172 176 - BsmAI GTCTC 166 170 . . 172 177 - Esp3I CGTCTC 166 170 . . 192 197 + BsmI GAATGC 198 196 . . 195 198 + CviRI* TGCA 196 196 . . 196 200 + BlsI GCNGC 198 197 . . 196 200 + BisI GCNGC 197 198 . . 196 200 + BbvI GCAGC 208 212 . . 196 200 + TseI GCWGC 196 199 . . 198 201 + SetI ASST 201 197 . . 198 201 + CviJI RGCY 199 199 . . 198 201 + AluI AGCT 199 199 . . 203 206 + MseI TTAA 203 205 . . 212 217 + NspBII* CMGCKG 214 214 . . 207 212 - BciVI GTATCC 201 200 . . 212 215 + AciI CCGC 212 214 . . 224 227 + AciI CCGC 224 226 . . 221 225 - HphI GGTGA 213 212 . . 236 246 + FalI AAGNNNNNCTT 227 222 259 254 236 246 - FalI AAGNNNNNCTT 259 254 227 222 260 263 + TaqI TCGA 260 262 . . 266 269 + TaiI ACGT 269 265 . . 266 269 + SetI ASST 269 265 . . 266 269 + MaeII ACGT 266 268 . . 268 273 + Hpy166II GTNNAC 270 270 . . 281 285 - BccI CCATC 275 276 . . 306 315 + MslI CAYNNNNRTG 310 310 . . 307 311 + Tsp4CI* ACNGT 309 308 . . 305 309 - HphI GGTGA 297 296 . . 312 315 + FatI CATG 311 315 . . 312 315 + NlaIII CATG 315 311 . . 312 315 + CviAII CATG 312 314 . . 314 319 - TaqII CACCCA 304 302 . . 328 333 + MfeI CAATTG 328 332 . . 329 332 + TspEI AATT 328 332 . . 333 337 - BccI CCATC 327 328 . . 339 342 + CviJI RGCY 340 340 . . 337 340 - MnlI CCTC 330 329 . . 344 349 + StyI CCWWGG 344 348 . . 344 349 + SecI* CCNNGG 344 348 . . 349 354 + NlaIV GGNNCC 351 351 . . 349 354 + HgiCI* GGYRCC 349 353 . . 354 363 + MslI CAYNNNNRTG 358 358 . . 354 357 + FatI CATG 353 357 . . 354 357 + NlaIII CATG 357 353 . . 354 357 + CviAII CATG 354 356 . . 359 364 + Hpy178III* TCNNGA 360 362 . . 359 364 + BspHI TCATGA 359 363 . . 360 363 + FatI CATG 359 363 . . 360 363 + NlaIII CATG 363 359 . . 360 363 + CviAII CATG 360 362 . . 382 385 + CviJI RGCY 383 383 . . 379 383 - BccI CCATC 373 374 . . 389 394 + BsgI GTGCAG 410 408 . . 390 393 + CviRI* TGCA 391 391 . . 391 395 + BlsI GCNGC 393 392 . . 391 395 + BisI GCNGC 392 393 . . 391 395 + BbvI GCAGC 403 407 . . 391 395 + TseI GCWGC 391 394 . . 393 396 + SetI ASST 396 392 . . 393 396 + CviJI RGCY 394 394 . . 393 396 + AluI AGCT 394 394 . . 422 426 + Tsp4CI* ACNGT 424 423 . . 420 424 - HphI GGTGA 412 411 . . 437 442 + PvuII CAGCTG 439 439 . . 437 442 + NspBII* CMGCKG 439 439 . . 438 441 + SetI ASST 441 437 . . 438 441 + CviJI RGCY 439 439 . . 438 441 + AluI AGCT 439 439 . . 434 439 - Eco57MI CTGRAG 419 417 . . 434 439 - GsuI CTGGAG 419 417 . . 445 449 + Tsp4CI* ACNGT 447 446 . . 479 482 + CviJI RGCY 480 480 . . 492 495 + TspEI AATT 491 495 . . 499 504 + AsuII TTCGAA 500 502 . . 500 503 + TaqI TCGA 500 502 . . 504 507 + SetI ASST 507 503 . . 502 506 - Hin4II* CCTTC 496 495 . . 506 509 + Csp6I GTAC 506 508 . . 506 509 + RsaI GTAC 507 507 . . 515 518 + TaqI TCGA 515 517 . . 505 515 - BaeI ACNNNNGTAYC 530 525 497 492 523 527 + BmgT120I GGNCC 524 525 . . 523 527 + AvaII GGWCC 523 526 . . 523 527 + AsuI* GGNCC 523 526 . . 521 526 - TaqII GACCGA 511 509 . . 537 540 + CviRI* TGCA 538 538 . . 540 543 + SetI ASST 543 539 . . 538 543 - BspMI ACCTGC 529 533 . . 552 556 + MaeIII GTNAC 551 556 . . 560 564 + MaeIII GTNAC 559 564 . . 566 569 + CviRI* TGCA 567 567 . . 568 573 + BtrI CACGTC 570 570 . . 569 572 + TaiI ACGT 572 568 . . 569 572 + SetI ASST 572 568 . . 569 572 + MaeII ACGT 569 571 . . 571 575 - BslFI GGGAC 556 560 . . 578 589 + BstXI CCANNNNNNTGG 585 581 . . 579 588 + MslI CAYNNNNRTG 583 583 . . 579 582 + FatI CATG 578 582 . . 579 582 + NlaIII CATG 582 578 . . 579 582 + CviAII CATG 579 581 . . 588 592 + FokI GGATG 601 605 . . 588 592 + BseGI GGATG 594 592 . . 589 593 - BccI CCATC 583 584 . . 606 609 + Csp6I GTAC 606 608 . . 606 609 + RsaI GTAC 607 607 . . 609 612 + FnuDII* CGCG 610 610 . . 610 613 - AciI CCGC 610 612 . . 618 623 + Hpy178III* TCNNGA 619 621 . . 622 626 + MboII GAAGA 634 633 . . 631 636 + Hpy166II GTNNAC 633 633 . . 639 644 + TaqII CACCCA 655 653 . . 652 662 + MwoI GCNNNNNNNGC 658 655 . . 701 704 + TaiI ACGT 704 700 . . 701 704 + SetI ASST 704 700 . . 701 704 + MaeII ACGT 701 703 . . 703 708 + Hpy166II GTNNAC 705 705 . . 707 711 + Tsp4CI* ACNGT 709 708 . . 708 714 + TspRI CASTGNN 714 705 . . 723 726 + SetI ASST 726 722 . . 723 729 + SexAI ACCWGGT 723 728 . . 724 728 + BssKI CCNGG 723 728 . . 724 728 + BseBI CCWGG 725 726 . . 724 728 + ScrFI CCNGG 725 726 . . 724 728 + EcoRII CCWGG 723 728 . . 730 733 + CviJI RGCY 731 731 . . 736 739 + MaeI CTAG 736 738 . . 738 741 + SetI ASST 741 737 . . 740 744 + MaeIII GTNAC 739 744 . . #--------------------------------------- # # # AciI: # [CCGC] # 12 140 212 386 # # AluI: # [AGCT] # 45 52 147 195 311 # # ApoI: # [RAATTY] # 119 631 # # AsuI*: # [GGNCC] # 227 523 # # AsuII: # [TTCGAA] # 250 500 # # AvaII: # [GGWCC] # 227 523 # # BaeI: # [ACNNNNGTAYC] # 33 220 497 # # BbvI: # [GCAGC] # 195 208 347 # # BccI: # [CCATC] # 46 52 167 210 275 # # Bce83I*: # [CTTGAG] # 98 652 # # BciVI: # [GTATCC] # 201 549 # # BisI: # [GCNGC] # 195 197 358 # # BlsI: # [GCNGC] # 195 198 357 # # BmgT120I: # [GGNCC] # 226 524 # # BseBI: # [CCWGG] # 25 725 # # BseGI: # [GGATG] # 156 594 # # BseMII: # [CTCAG] # 83 667 # # BsgI: # [GTGCAG] # 340 410 # # BslFI: # [GGGAC] # 194 556 # # BsmAI: # [GTCTC] # 166 584 # # BsmI: # [GAATGC] # 198 552 # # BspCNI: # [CTCAG] # 84 666 # # BspHI: # [TCATGA] # 359 391 # # BspMI: # [ACCTGC] # 221 529 # # BssKI: # [CCNGG] # 27 723 # # BstAPI: # [GCANNNNNTGC] # 40 710 # # BstXI: # [CCANNNNNNTGG] # 165 585 # # BtrI: # [CACGTC] # 180 570 # # Csp6I: # [GTAC] # 100 144 506 # # CviAII: # [CATG] # 6 42 171 219 312 # # CviJI: # [RGCY] # 11 19 41 43 45 52 # 141 147 251 # # CviRI*: # [TGCA] # 29 34 147 162 183 195 # # DdeI: # [CTNAG] # 92 658 # # Eco57MI: # [CTGRAG] # 331 419 # # EcoP15I: # [CAGCAG] # 61 689 # # EcoRII: # [CCWGG] # 27 723 # # Esp3I: # [CGTCTC] # 166 584 # # FalI: # [AAGNNNNNCTT] # 0 0 32 227 491 # # FatI: # [CATG] # 6 42 172 219 311 # # FnuDII*: # [CGCG] # 140 610 # # FokI: # [GGATG] # 149 601 # # GsuI: # [CTGGAG] # 331 419 # # HgiAI*: # [GWGCWC] # 63 687 # # HgiCI*: # [GGYRCC] # 349 401 # # Hin4I: # [GAYNNNNNVTC] # 14 18 18 67 633 # # Hin4II*: # [CCTTC] # 116 254 380 # # HinfI: # [GANTC] # 132 618 # # HpaII: # [CCGG] # 14 736 # # HphI: # [GGTGA] # 84 115 213 338 # # Hpy166II: # [GTNNAC] # 45 72 270 363 # # Hpy178III*: # [TCNNGA] # 115 131 245 259 # # Ksp632I*: # [CTCTTC] # 15 735 # # MaeI: # [CTAG] # 14 49 687 # # MaeII: # [ACGT] # 49 132 266 303 # # MaeIII: # [GTNAC] # 8 11 29 38 66 180 # 418 # # MboII: # [GAAGA] # 2 116 632 # # MfeI: # [CAATTG] # 328 422 # # MlyI: # [GAGTC] # 141 609 # # MmeI: # [TCCRAC] # 109 641 # # MnlI: # [CCTC] # 330 420 # # MseI: # [TTAA] # 203 547 # # MslI: # [CAYNNNNRTG] # 48 167 225 310 # # MwoI: # [GCNNNNNNNGC] # 9 9 40 92 600 # # NlaIII: # [CATG] # 6 42 168 219 315 # # NlaIV: # [GGNNCC] # 351 399 # # NspBII*: # [CMGCKG] # 214 225 311 # # PleI: # [GAGTC] # 140 610 # # PvuII: # [CAGCTG] # 311 439 # # RsaI: # [GTAC] # 100 143 507 # # ScrFI: # [CCNGG] # 25 725 # # SduI: # [GDGCHC] # 63 687 # # SecI*: # [CCNNGG] # 344 406 # # SetI: # [ASST] # 9 15 22 29 35 36 # 45 54 66 68 112 127 # 132 # # SexAI: # [ACCWGGT] # 27 723 # # SmlI: # [CTYRAG] # 113 637 # # StyI: # [CCWWGG] # 344 406 # # TaiI: # [ACGT] # 46 132 269 303 # # TaqI: # [TCGA] # 15 235 240 260 # # TaqII: # [CACCCA] # 95 144 207 304 # # TseI: # [GCWGC] # 195 196 359 # # Tsp45I: # [GTSAC] # 66 67 617 # # Tsp4CI*: # [ACNGT] # 23 41 115 262 309 # # TspDTI: # [ATGAA] # 16 82 652 # # TspEI: # [AATT] # 119 163 209 259 # # TspRI: # [CASTGNN] # 36 714 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 750 # Reported_sequences: 1 # Reported_hitcount: 167 #---------------------------------------