Restriction Fragments for SPT20/YOL148C



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The currently selected gene/sequence is :

SPT20/YOL148C on chromosome XV from coordinates 47574 to 45760.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.27361.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 13 16 + CviJI RGCY 14 14 . . 28 31 + DpnI GATC 29 29 . . 28 31 + BstKTI GATC 30 28 . . 28 31 + MboI GATC 27 31 . . 28 32 - BinI* GGATC 22 23 . . 33 44 + BstXI CCANNNNNNTGG 40 36 . . 34 37 + FatI CATG 33 37 . . 34 37 + NlaIII CATG 37 33 . . 34 37 + CviAII CATG 34 36 . . 52 57 + Hpy166II GTNNAC 54 54 . . 64 68 + BccI CCATC 72 73 . . 65 69 - FokI GGATG 51 55 . . 65 69 - BseGI GGATG 64 62 . . 82 86 + BssKI CCNGG 81 86 . . 82 86 + BseBI CCWGG 83 84 . . 82 86 + ScrFI CCNGG 83 84 . . 82 86 + EcoRII CCWGG 81 86 . . 95 98 + MseI TTAA 95 97 . . 91 95 - BsrI ACTGG 91 89 . . 100 103 + Csp6I GTAC 100 102 . . 100 103 + RsaI GTAC 101 101 . . 105 108 + SetI ASST 108 104 . . 106 109 + MnlI CCTC 116 115 . . 112 116 + TspDTI ATGAA 127 125 . . 117 122 + GsaI CCCAGC 121 117 . . 117 122 + BseYI CCCAGC 117 121 . . 119 124 + NspBII* CMGCKG 121 121 . . 121 124 - AciI CCGC 121 123 . . 139 143 - FokI GGATG 125 129 . . 139 143 - BseGI GGATG 138 136 . . 150 153 - MnlI CCTC 143 142 . . 158 162 + Tsp4CI* ACNGT 160 159 . . 176 180 + TspDTI ATGAA 191 189 . . 180 183 + SetI ASST 183 179 . . 178 182 - Hin4II* CCTTC 172 171 . . 182 185 + Csp6I GTAC 182 184 . . 182 185 + RsaI GTAC 183 183 . . 185 188 + MaeI CTAG 185 187 . . 193 196 + MseI TTAA 193 195 . . 194 199 + TsoI TARCCA 210 208 . . 197 201 + BssKI CCNGG 196 201 . . 197 201 + BseBI CCWGG 198 199 . . 197 202 + SecI* CCNNGG 197 201 . . 197 201 + ScrFI CCNGG 198 199 . . 197 201 + EcoRII CCWGG 196 201 . . 204 214 + MwoI GCNNNNNNNGC 210 207 . . 213 223 + MwoI GCNNNNNNNGC 219 216 . . 214 219 + MfeI CAATTG 214 218 . . 215 218 + TspEI AATT 214 218 . . 218 221 + CviRI* TGCA 219 219 . . 219 223 + BlsI GCNGC 221 220 . . 219 223 + BisI GCNGC 220 221 . . 219 223 + BbvI GCAGC 231 235 . . 219 223 + TseI GCWGC 219 222 . . 241 246 + SfeI* CTRYAG 241 245 . . 257 260 + TaqI TCGA 257 259 . . 276 281 + Cac8I GCNNGC 278 278 . . 297 300 + CviJI RGCY 298 298 . . 295 298 - MnlI CCTC 288 287 . . 299 303 + DdeI CTNAG 299 302 . . 299 303 + BspCNI CTCAG 312 310 . . 299 303 + BseMII CTCAG 313 311 . . 322 325 + SetI ASST 325 321 . . 325 328 + MseI TTAA 325 327 . . 336 339 + SetI ASST 339 335 . . 352 355 + TspEI AATT 351 355 . . 361 365 + MboII GAAGA 373 372 . . 364 374 + Hin4I GAYNNNNNVTC 355 350 387 382 366 369 + CviRI* TGCA 367 367 . . 364 368 - SfaNI GCATC 354 358 . . 370 374 + HinfI GANTC 370 373 . . 370 374 - PleI GAGTC 364 365 . . 370 374 - MlyI GAGTC 364 364 . . 375 378 + TspEI AATT 374 378 . . 395 400 + SspI AATATT 397 397 . . 404 408 + Tsp4CI* ACNGT 406 405 . . 412 417 + ApoI RAATTY 412 416 . . 413 416 + TspEI AATT 412 416 . . 418 427 + MslI CAYNNNNRTG 422 422 . . 416 420 - TspDTI ATGAA 406 404 . . 433 436 + TspEI AATT 432 436 . . 435 440 + PsiI TTATAA 437 437 . . 441 451 + Hin4I GAYNNNNNVTC 432 427 464 459 446 449 + Hin6I GCGC 446 448 . . 446 449 + GlaI GCGC 447 447 . . 446 449 + HhaI GCGC 448 446 . . 453 456 + CviRI* TGCA 454 454 . . 463 466 + SetI ASST 466 462 . . 465 469 + MaeIII GTNAC 464 469 . . 461 466 - BspMI ACCTGC 452 456 . . 472 477 + EcoP15I CAGCAG 502 504 . . 474 484 + MwoI GCNNNNNNNGC 480 477 . . 474 478 + BlsI GCNGC 476 475 . . 474 478 + BisI GCNGC 475 476 . . 474 478 + BbvI GCAGC 486 490 . . 474 478 + TseI GCWGC 474 477 . . 483 493 + MwoI GCNNNNNNNGC 489 486 . . 487 492 + BmeT110I CYCGRG 488 490 . . 487 492 + AvaI CYCGRG 487 491 . . 491 494 + SetI ASST 494 490 . . 491 494 + CviJI RGCY 492 492 . . 491 494 + AluI AGCT 492 492 . . 496 501 + SpeI ACTAGT 496 500 . . 497 500 + MaeI CTAG 497 499 . . 500 504 + Tsp45I GTSAC 499 504 . . 500 504 + MaeIII GTNAC 499 504 . . 503 507 + Tsp4CI* ACNGT 505 504 . . 508 511 + MseI TTAA 508 510 . . 510 515 + SspI AATATT 512 512 . . 514 517 + MseI TTAA 514 516 . . 515 520 + TsoI TARCCA 531 529 . . 541 546 + ApoI RAATTY 541 545 . . 542 545 + TspEI AATT 541 545 . . 552 555 + Csp6I GTAC 552 554 . . 552 555 + RsaI GTAC 553 553 . . 554 557 + TaiI ACGT 557 553 . . 554 557 + SetI ASST 557 553 . . 554 557 + MaeII ACGT 554 556 . . 555 559 + Hpy99I CGWCG 559 554 . . 563 566 - MnlI CCTC 556 555 . . 570 574 + TfiI GAWTC 570 573 . . 566 570 - Hin4II* CCTTC 560 559 . . 570 574 + HinfI GANTC 570 573 . . 573 578 + Hpy178III* TCNNGA 574 576 . . 577 581 - SfaNI GCATC 567 571 . . 594 599 + SmlI CTYRAG 594 598 . . 594 599 + AflII CTTAAG 594 598 . . 595 598 + MseI TTAA 595 597 . . 599 603 + BslFI GGGAC 613 617 . . 602 606 + Tsp4CI* ACNGT 604 603 . . 614 617 + TspEI AATT 613 617 . . 623 633 + FalI AAGNNNNNCTT 614 609 646 641 622 626 - Hin4II* CCTTC 616 615 . . 623 633 - FalI AAGNNNNNCTT 646 641 614 609 641 644 + SetI ASST 644 640 . . 661 665 + Tsp4CI* ACNGT 663 662 . . 670 674 + Tsp45I GTSAC 669 674 . . 670 674 + MaeIII GTNAC 669 674 . . 671 675 - HphI GGTGA 663 662 . . 689 692 + CviJI RGCY 690 690 . . 694 697 + TspEI AATT 693 697 . . 703 706 + SetI ASST 706 702 . . 703 706 + CviJI RGCY 704 704 . . 703 706 + AluI AGCT 704 704 . . 704 710 + SapI GCTCTTC 711 714 . . 705 710 + Ksp632I* CTCTTC 711 714 . . 706 710 - MboII GAAGA 698 697 . . 709 713 - MboII GAAGA 701 700 . . 715 719 + Hin4II* CCTTC 725 724 . . 742 745 + TspEI AATT 741 745 . . 750 754 + TfiI GAWTC 750 753 . . 750 754 + HinfI GANTC 750 753 . . 760 764 + BccI CCATC 768 769 . . 763 767 + Hpy188I TCNGA 765 764 . . 761 765 - FokI GGATG 747 751 . . 761 765 - BseGI GGATG 760 758 . . 768 771 + SetI ASST 771 767 . . 769 779 + BsiYI* CCNNNNNNNGG 775 772 . . 794 797 + Hin6I GCGC 794 796 . . 794 797 + GlaI GCGC 795 795 . . 794 797 + HhaI GCGC 796 794 . . 796 806 + MwoI GCNNNNNNNGC 802 799 . . 814 824 + FalI AAGNNNNNCTT 805 800 837 832 817 821 + SfaNI GCATC 826 830 . . 813 817 - Hin4II* CCTTC 807 806 . . 821 825 + Hin4II* CCTTC 831 830 . . 818 822 - FokI GGATG 804 808 . . 818 822 - BseGI GGATG 817 815 . . 814 824 - FalI AAGNNNNNCTT 837 832 805 800 831 834 + SetI ASST 834 830 . . 833 836 + MaeI CTAG 833 835 . . 843 846 + TaqI TCGA 843 845 . . 857 860 + CviJI RGCY 858 858 . . 872 876 + Hpy99I CGWCG 876 871 . . 874 877 + TaiI ACGT 877 873 . . 874 877 + SetI ASST 877 873 . . 874 877 + MaeII ACGT 874 876 . . 883 893 + Hin4I GAYNNNNNVTC 874 869 906 901 883 893 + Eam1105I GACNNNNNGTC 888 887 . . 885 888 + FatI CATG 884 888 . . 885 888 + NlaIII CATG 888 884 . . 885 888 + CviAII CATG 885 887 . . 883 893 - Hin4I GAYNNNNNVTC 906 901 874 869 897 900 + CviRI* TGCA 898 898 . . 898 908 + MwoI GCNNNNNNNGC 904 901 . . 898 908 + BstAPI GCANNNNNTGC 904 901 . . 908 912 + BssKI CCNGG 907 912 . . 908 912 + BseBI CCWGG 909 910 . . 908 912 + ScrFI CCNGG 909 910 . . 908 912 + EcoRII CCWGG 907 912 . . 910 913 + SetI ASST 913 909 . . 916 920 + Hpy188I TCNGA 918 917 . . 933 937 + Tsp4CI* ACNGT 935 934 . . 940 944 + TfiI GAWTC 940 943 . . 940 944 + HinfI GANTC 940 943 . . 943 947 + Hpy188I TCNGA 945 944 . . 947 952 + ApoI RAATTY 947 951 . . 948 951 + TspEI AATT 947 951 . . 952 955 + MseI TTAA 952 954 . . 964 974 + FalI AAGNNNNNCTT 955 950 987 982 966 969 - MnlI CCTC 959 958 . . 964 974 - FalI AAGNNNNNCTT 987 982 955 950 975 979 + Tsp45I GTSAC 974 979 . . 975 979 + MaeIII GTNAC 974 979 . . 977 985 + DraIII CACNNNGTG 982 979 . . 979 983 + DdeI CTNAG 979 982 . . 991 994 + MseI TTAA 991 993 . . 998 1003 + NdeI CATATG 999 1001 . . 1003 1008 + SduI GDGCHC 1007 1003 . . 1003 1008 + HgiAI* GWGCWC 1007 1003 . . 1013 1018 + BspLU11I* ACATGT 1013 1017 . . 1013 1018 + NspI RCATGY 1017 1013 . . 1013 1018 + AflIII ACRYGT 1013 1017 . . 1014 1017 + FatI CATG 1013 1017 . . 1014 1017 + NlaIII CATG 1017 1013 . . 1014 1017 + CviAII CATG 1014 1016 . . 1010 1014 - BsmAI GTCTC 1004 1008 . . 1017 1022 - MmeI TCCRAC 998 996 . . 1021 1024 - MnlI CCTC 1014 1013 . . 1029 1032 + SetI ASST 1032 1028 . . 1029 1032 + CviJI RGCY 1030 1030 . . 1029 1032 + AluI AGCT 1030 1030 . . 1036 1040 + Hpy188I TCNGA 1038 1037 . . 1041 1044 + SetI ASST 1044 1040 . . 1042 1045 + MnlI CCTC 1052 1051 . . 1067 1071 + Hin4II* CCTTC 1077 1076 . . 1069 1073 - TspDTI ATGAA 1059 1057 . . 1074 1079 + Hpy178III* TCNNGA 1075 1077 . . 1077 1088 + BcgI CGANNNNNNTGC 1066 1064 1100 1098 1088 1092 + DdeI CTNAG 1088 1091 . . 1090 1094 + PleI GAGTC 1098 1099 . . 1090 1094 + MlyI GAGTC 1099 1099 . . 1090 1094 + HinfI GANTC 1090 1093 . . 1092 1097 + Hpy166II GTNNAC 1094 1094 . . 1088 1092 - BspCNI CTCAG 1080 1078 . . 1088 1092 - BseMII CTCAG 1079 1077 . . 1092 1097 + AccI GTMKAC 1093 1095 . . 1102 1106 - Hin4II* CCTTC 1096 1095 . . 1106 1116 + MwoI GCNNNNNNNGC 1112 1109 . . 1110 1114 - Hin4II* CCTTC 1104 1103 . . 1130 1133 + TaqI TCGA 1130 1132 . . 1132 1136 + MboII GAAGA 1144 1143 . . 1138 1141 + FnuDII* CGCG 1139 1139 . . 1139 1144 + BtgZI GCGATG 1154 1158 . . 1142 1146 + TspDTI ATGAA 1157 1155 . . 1144 1149 + ApoI RAATTY 1144 1148 . . 1145 1148 + TspEI AATT 1144 1148 . . 1150 1153 + AciI CCGC 1150 1152 . . 1165 1168 + TspEI AATT 1164 1168 . . 1169 1173 + TspDTI ATGAA 1184 1182 . . 1173 1177 + Tsp4CI* ACNGT 1175 1174 . . 1178 1189 + BdaI TGANNNNNNTCA 1167 1165 1201 1199 1179 1188 + BsaBI GATNNNNATC 1183 1183 . . 1184 1189 + BclI TGATCA 1184 1188 . . 1185 1188 + DpnI GATC 1186 1186 . . 1185 1188 + BstKTI GATC 1187 1185 . . 1185 1188 + MboI GATC 1184 1188 . . 1187 1192 + Hpy178III* TCNNGA 1188 1190 . . 1187 1192 + BspHI TCATGA 1187 1191 . . 1188 1191 + FatI CATG 1187 1191 . . 1188 1191 + NlaIII CATG 1191 1187 . . 1188 1191 + CviAII CATG 1188 1190 . . 1189 1193 + TspDTI ATGAA 1204 1202 . . 1178 1189 - BdaI TGANNNNNNTCA 1201 1199 1167 1165 1193 1197 + TspDTI ATGAA 1208 1206 . . 1211 1216 + TsoI TARCCA 1227 1225 . . 1222 1225 + TaiI ACGT 1225 1221 . . 1222 1225 + SetI ASST 1225 1221 . . 1222 1225 + MaeII ACGT 1222 1224 . . 1236 1241 + Hpy166II GTNNAC 1238 1238 . . 1236 1241 + HpaI GTTAAC 1238 1238 . . 1236 1241 + HindII GTYRAC 1238 1238 . . 1237 1240 + MseI TTAA 1237 1239 . . 1238 1243 + TsoI TARCCA 1254 1252 . . 1241 1255 + XcmI CCANNNNNNNNNTGG 1248 1247 . . 1245 1250 + BsmI GAATGC 1251 1249 . . 1249 1254 + BtgZI GCGATG 1264 1268 . . 1251 1255 - BccI CCATC 1245 1246 . . 1261 1266 + Cac8I GCNNGC 1263 1263 . . 1261 1266 + BmtI GCTAGC 1265 1261 . . 1261 1266 + NheI GCTAGC 1261 1265 . . 1262 1265 + MaeI CTAG 1262 1264 . . 1264 1267 + SetI ASST 1267 1263 . . 1264 1267 + CviJI RGCY 1265 1265 . . 1264 1267 + AluI AGCT 1265 1265 . . 1266 1271 - Eco57MI CTGRAG 1251 1249 . . 1266 1271 - GsuI CTGGAG 1251 1249 . . 1268 1272 - BsrI ACTGG 1268 1266 . . 1273 1278 + MmeI TCCRAC 1298 1296 . . 1282 1285 + Csp6I GTAC 1282 1284 . . 1282 1285 + RsaI GTAC 1283 1283 . . 1283 1288 + BsaAI YACGTR 1285 1285 . . 1284 1287 + TaiI ACGT 1287 1283 . . 1284 1287 + SetI ASST 1287 1283 . . 1284 1287 + MaeII ACGT 1284 1286 . . 1290 1293 + SetI ASST 1293 1289 . . 1290 1293 + CviJI RGCY 1291 1291 . . 1290 1293 + AluI AGCT 1291 1291 . . 1298 1301 + MnlI CCTC 1308 1307 . . 1305 1310 + AsuII TTCGAA 1306 1308 . . 1306 1309 + TaqI TCGA 1306 1308 . . 1308 1313 + EcoRI GAATTC 1308 1312 . . 1308 1313 + ApoI RAATTY 1308 1312 . . 1309 1312 + TspEI AATT 1308 1312 . . 1313 1316 + AciI CCGC 1313 1315 . . 1315 1319 + SfaNI GCATC 1324 1328 . . 1329 1342 + AjuI GAANNNNNNNTTGG 1321 1316 1353 1348 1334 1338 + Tsp4CI* ACNGT 1336 1335 . . 1341 1344 + CviJI RGCY 1342 1342 . . 1340 1345 - TsoI TARCCA 1330 1328 . . 1359 1362 + SetI ASST 1362 1358 . . 1359 1362 + CviJI RGCY 1360 1360 . . 1359 1362 + AluI AGCT 1360 1360 . . 1360 1370 + MwoI GCNNNNNNNGC 1366 1363 . . 1366 1376 + MwoI GCNNNNNNNGC 1372 1369 . . 1366 1370 + BlsI GCNGC 1368 1367 . . 1366 1370 + BisI GCNGC 1367 1368 . . 1366 1370 + BbvI GCAGC 1378 1382 . . 1366 1370 + TseI GCWGC 1366 1369 . . 1367 1372 + NspBII* CMGCKG 1369 1369 . . 1369 1373 + TauI GCSGC 1372 1369 . . 1369 1379 + MwoI GCNNNNNNNGC 1375 1372 . . 1369 1373 + BlsI GCNGC 1371 1370 . . 1369 1373 + BisI GCNGC 1370 1371 . . 1372 1382 + MwoI GCNNNNNNNGC 1378 1375 . . 1372 1382 + BstAPI GCANNNNNTGC 1378 1375 . . 1372 1376 + BlsI GCNGC 1374 1373 . . 1372 1376 + BisI GCNGC 1373 1374 . . 1372 1376 + BbvI GCAGC 1384 1388 . . 1372 1376 + TseI GCWGC 1372 1375 . . 1369 1372 - AciI CCGC 1369 1371 . . 1373 1378 + NspBII* CMGCKG 1375 1375 . . 1373 1381 + AlwNI CAGNNNCTG 1378 1375 . . 1375 1379 + TauI GCSGC 1378 1375 . . 1375 1385 + MwoI GCNNNNNNNGC 1381 1378 . . 1375 1379 + BlsI GCNGC 1377 1376 . . 1375 1379 + BisI GCNGC 1376 1377 . . 1377 1380 + CviJI RGCY 1378 1378 . . 1378 1388 + MwoI GCNNNNNNNGC 1384 1381 . . 1378 1382 + BlsI GCNGC 1380 1379 . . 1378 1382 + BisI GCNGC 1379 1380 . . 1378 1382 + TseI GCWGC 1378 1381 . . 1375 1378 - AciI CCGC 1375 1377 . . 1381 1385 + TauI GCSGC 1384 1381 . . 1381 1385 + BlsI GCNGC 1383 1382 . . 1381 1385 + BisI GCNGC 1382 1383 . . 1378 1382 - BbvI GCAGC 1365 1369 . . 1383 1386 + CviJI RGCY 1384 1384 . . 1384 1388 + BlsI GCNGC 1386 1385 . . 1384 1388 + BisI GCNGC 1385 1386 . . 1384 1388 + TseI GCWGC 1384 1387 . . 1381 1384 - AciI CCGC 1381 1383 . . 1385 1390 + PstI CTGCAG 1389 1385 . . 1385 1390 + SfeI* CTRYAG 1385 1389 . . 1386 1389 + CviRI* TGCA 1387 1387 . . 1387 1392 + BtsI GCAGTG 1394 1392 . . 1388 1394 + TspRI CASTGNN 1394 1385 . . 1384 1388 - BbvI GCAGC 1371 1375 . . 1393 1398 + XhoII RGATCY 1393 1397 . . 1393 1397 + BinI* GGATC 1401 1402 . . 1394 1397 + DpnI GATC 1395 1395 . . 1394 1397 + BstKTI GATC 1396 1394 . . 1394 1397 + MboI GATC 1393 1397 . . 1400 1405 + DsaI* CCRYGG 1400 1404 . . 1400 1405 + StyI CCWWGG 1400 1404 . . 1400 1405 + NcoI CCATGG 1400 1404 . . 1400 1405 + SecI* CCNNGG 1400 1404 . . 1401 1404 + FatI CATG 1400 1404 . . 1401 1404 + NlaIII CATG 1404 1400 . . 1401 1404 + CviAII CATG 1401 1403 . . 1406 1410 + MaeIII GTNAC 1405 1410 . . 1411 1420 + BsaBI GATNNNNATC 1415 1415 . . 1421 1424 + TspEI AATT 1420 1424 . . 1427 1432 + Cac8I GCNNGC 1429 1429 . . 1430 1433 + CviJI RGCY 1431 1431 . . 1435 1440 + ApoI RAATTY 1435 1439 . . 1436 1439 + TspEI AATT 1435 1439 . . 1477 1482 + EcoP15I CAGCAG 1507 1509 . . 1479 1484 + Cac8I GCNNGC 1481 1481 . . 1482 1485 + CviJI RGCY 1483 1483 . . 1483 1493 + MwoI GCNNNNNNNGC 1489 1486 . . 1486 1489 + MseI TTAA 1486 1488 . . 1491 1494 + CviRI* TGCA 1492 1492 . . 1503 1508 + MmeI TCCRAC 1528 1526 . . 1511 1515 + Hin4II* CCTTC 1521 1520 . . 1520 1523 + MaeI CTAG 1520 1522 . . 1527 1532 + ClaI ATCGAT 1528 1530 . . 1528 1531 + TaqI TCGA 1528 1530 . . 1545 1549 + HinfI GANTC 1545 1548 . . 1545 1549 - PleI GAGTC 1539 1540 . . 1545 1549 - MlyI GAGTC 1539 1539 . . 1559 1562 + SetI ASST 1562 1558 . . 1559 1562 + CviJI RGCY 1560 1560 . . 1559 1562 + AluI AGCT 1560 1560 . . 1576 1581 + EcoP15I CAGCAG 1606 1608 . . 1578 1583 + Cac8I GCNNGC 1580 1580 . . 1580 1585 + StuI AGGCCT 1582 1582 . . 1581 1584 + CviJI RGCY 1582 1582 . . 1581 1584 + HaeIII GGCC 1582 1582 . . 1583 1588 + StyI CCWWGG 1583 1587 . . 1583 1588 + SecI* CCNNGG 1583 1587 . . 1606 1611 + NlaIV GGNNCC 1608 1608 . . 1608 1611 + CviJI RGCY 1609 1609 . . 1611 1614 + FatI CATG 1610 1614 . . 1611 1614 + NlaIII CATG 1614 1610 . . 1611 1614 + CviAII CATG 1611 1613 . . 1612 1616 + TspDTI ATGAA 1627 1625 . . 1629 1633 + TspGWI ACGGA 1644 1642 . . 1637 1641 + MaeIII GTNAC 1636 1641 . . 1652 1656 + MaeIII GTNAC 1651 1656 . . 1649 1653 - BsrI ACTGG 1649 1647 . . 1675 1678 + TspEI AATT 1674 1678 . . 1691 1694 + SetI ASST 1694 1690 . . 1694 1697 + MseI TTAA 1694 1696 . . 1701 1704 + SetI ASST 1704 1700 . . 1703 1706 + MaeI CTAG 1703 1705 . . 1713 1716 + CviRI* TGCA 1714 1714 . . 1733 1738 + Hpy166II GTNNAC 1735 1735 . . 1733 1736 + Csp6I GTAC 1733 1735 . . 1733 1736 + RsaI GTAC 1734 1734 . . 1730 1733 - AciI CCGC 1730 1732 . . 1728 1733 - BsrBI CCGCTC 1730 1730 . . 1735 1745 + BsaXI ACNNNNNCTCC 1725 1722 1755 1752 1740 1743 + SetI ASST 1743 1739 . . 1740 1743 + CviJI RGCY 1741 1741 . . 1740 1743 + AluI AGCT 1741 1741 . . 1763 1767 + DdeI CTNAG 1763 1766 . . 1767 1771 + MboII GAAGA 1779 1778 . . 1778 1781 + Hin6I GCGC 1778 1780 . . 1778 1781 + GlaI GCGC 1779 1779 . . 1778 1788 + MwoI GCNNNNNNNGC 1784 1781 . . 1778 1781 + HhaI GCGC 1780 1778 . . 1787 1792 + Cac8I GCNNGC 1789 1789 . . 1793 1796 + MnlI CCTC 1803 1802 . . 1794 1799 + Ksp632I* CTCTTC 1800 1803 . . 1795 1799 - MboII GAAGA 1787 1786 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 722 1093 # # AciI: # [CCGC] # 6 6 56 85 121 163 # 349 1029 # # AflII: # [CTTAAG] # 594 1221 # # AflIII: # [ACRYGT] # 802 1013 # # AjuI: # [GAANNNNNNNTTGG] # 32 462 1321 # # AluI: # [AGCT] # 26 69 74 181 200 212 # 235 326 492 # # AlwNI: # [CAGNNNCTG] # 437 1378 # # ApoI: # [RAATTY] # 127 129 164 197 380 406 # 412 # # AsuII: # [TTCGAA] # 509 1306 # # AvaI: # [CYCGRG] # 487 1328 # # BbvI: # [GCAGC] # 6 6 7 231 255 431 # 879 # # BccI: # [CCATC] # 72 477 570 696 # # BcgI: # [CGANNNNNNTGC] # 34 715 1066 # # BclI: # [TGATCA] # 631 1184 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 614 1167 # # BinI*: # [GGATC] # 22 414 1379 # # BisI: # [GCNGC] # 3 3 3 3 3 3 # 220 255 430 892 # # BlsI: # [GCNGC] # 3 3 3 3 3 3 # 221 255 429 892 # # BmeT110I: # [CYCGRG] # 488 1327 # # BmtI: # [GCTAGC] # 550 1265 # # BsaAI: # [YACGTR] # 530 1285 # # BsaBI: # [GATNNNNATC] # 232 400 1183 # # BsaXI: # [ACNNNNNCTCC] # 30 60 1725 # # BseBI: # [CCWGG] # 83 115 711 906 # # BseGI: # [GGATG] # 57 64 74 622 998 # # BseMII: # [CTCAG] # 313 736 766 # # BseYI: # [CCCAGC] # 117 1698 # # BsiYI*: # [CCNNNNNNNGG] # 775 1040 # # BslFI: # [GGGAC] # 613 1202 # # BsmAI: # [GTCTC] # 811 1004 # # BsmI: # [GAATGC] # 564 1251 # # BspCNI: # [CTCAG] # 312 735 768 # # BspHI: # [TCATGA] # 628 1187 # # BspLU11I*: # [ACATGT] # 802 1013 # # BspMI: # [ACCTGC] # 452 1363 # # BsrBI: # [CCGCTC] # 85 1730 # # BsrI: # [ACTGG] # 91 166 381 1177 # # BssKI: # [CCNGG] # 81 115 711 908 # # BstAPI: # [GCANNNNNTGC] # 437 474 904 # # BstKTI: # [GATC] # 30 209 419 1157 # # BstXI: # [CCANNNNNNTGG] # 40 1775 # # BtgZI: # [GCGATG] # 110 551 1154 # # BtsI: # [GCAGTG] # 421 1394 # # Cac8I: # [GCNNGC] # 26 52 99 166 209 278 # 985 # # ClaI: # [ATCGAT] # 287 1528 # # Csp6I: # [GTAC] # 82 82 100 370 451 730 # # CviAII: # [CATG] # 34 129 174 204 210 213 # 851 # # CviJI: # [RGCY] # 6 14 14 18 18 22 # 26 27 47 51 52 74 # 77 132 154 172 194 198 # 235 284 # # CviRI*: # [TGCA] # 87 101 105 148 219 222 # 444 489 # # DdeI: # [CTNAG] # 52 109 299 675 680 # # DpnI: # [GATC] # 29 209 420 1157 # # DraIII: # [CACNNNGTG] # 833 982 # # DsaI*: # [CCRYGG] # 415 1400 # # Eam1105I: # [GACNNNNNGTC] # 888 927 # # Eco57MI: # [CTGRAG] # 564 1251 # # EcoP15I: # [CAGCAG] # 99 209 502 1005 # # EcoRI: # [GAATTC] # 507 1308 # # EcoRII: # [CCWGG] # 81 115 711 908 # # FalI: # [AAGNNNNNCTT] # 0 0 0 0 0 0 # 32 32 32 118 159 614 # 828 # # FatI: # [CATG] # 33 129 174 205 210 213 # 851 # # FnuDII*: # [CGCG] # 676 1139 # # FokI: # [GGATG] # 51 57 74 622 1011 # # GlaI: # [GCGC] # 36 348 447 984 # # GsaI: # [CCCAGC] # 121 1694 # # GsuI: # [CTGGAG] # 564 1251 # # HaeIII: # [GGCC] # 233 1582 # # HgiAI*: # [GWGCWC] # 808 1007 # # HhaI: # [GCGC] # 35 348 448 984 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 32 32 45 # 355 410 909 # # Hin4II*: # [CCTTC] # 8 19 24 56 82 109 # 172 246 294 388 417 # # Hin6I: # [GCGC] # 37 348 446 984 # # HindII: # [GTYRAC] # 577 1238 # # HinfI: # [GANTC] # 150 180 190 200 270 370 # 455 # # HpaI: # [GTTAAC] # 577 1238 # # HphI: # [GGTGA] # 663 1152 # # Hpy166II: # [GTNNAC] # 54 80 144 497 1040 # # Hpy178III*: # [TCNNGA] # 113 501 574 627 # # Hpy188I: # [TCNGA] # 27 93 153 765 777 # # Hpy99I: # [CGWCG] # 317 559 939 # # Ksp632I*: # [CTCTTC] # 15 711 1089 # # MaeI: # [CTAG] # 112 183 185 258 312 336 # 429 # # MaeII: # [ACGT] # 62 320 348 531 554 # # MaeIII: # [GTNAC] # 15 35 164 170 231 305 # 431 464 # # MboI: # [GATC] # 27 209 422 1157 # # MboII: # [GAAGA] # 3 8 28 325 373 443 # 635 # # MfeI: # [CAATTG] # 214 1601 # # MlyI: # [GAGTC] # 276 364 440 735 # # MmeI: # [TCCRAC] # 230 287 300 998 # # MnlI: # [CCTC] # 12 27 38 55 116 145 # 256 268 403 495 # # MseI: # [TTAA] # 6 39 81 95 98 121 # 132 183 208 246 249 357 # # MslI: # [CAYNNNNRTG] # 422 1393 # # MwoI: # [GCNNNNNNNGC] # 3 3 3 3 6 9 # 9 31 102 105 208 210 # 254 261 295 313 # # NcoI: # [CCATGG] # 415 1400 # # NdeI: # [CATATG] # 816 999 # # NheI: # [GCTAGC] # 554 1261 # # NlaIII: # [CATG] # 37 129 174 201 210 213 # 851 # # NlaIV: # [GGNNCC] # 207 1608 # # NspBII*: # [CMGCKG] # 6 121 440 1248 # # NspI: # [RCATGY] # 798 1017 # # PleI: # [GAGTC] # 276 364 441 734 # # PsiI: # [TTATAA] # 437 1378 # # PstI: # [CTGCAG] # 426 1389 # # RsaI: # [GTAC] # 81 82 101 370 451 730 # # SapI: # [GCTCTTC] # 711 1104 # # ScrFI: # [CCNGG] # 83 115 711 906 # # SduI: # [GDGCHC] # 808 1007 # # SecI*: # [CCNNGG] # 183 197 232 1203 # # SetI: # [ASST] # 6 10 12 14 20 28 # 36 39 42 43 62 63 # 63 65 69 72 75 87 # 108 119 127 132 142 181 # 200 # # SfaNI: # [GCATC] # 213 259 354 491 498 # # SfeI*: # [CTRYAG] # 241 430 1144 # # SmlI: # [CTYRAG] # 594 1221 # # SpeI: # [ACTAGT] # 496 1319 # # SspI: # [AATATT] # 115 397 1303 # # StuI: # [AGGCCT] # 233 1582 # # StyI: # [CCWWGG] # 183 232 1400 # # TaiI: # [ACGT] # 62 320 348 528 557 # # TaqI: # [TCGA] # 176 222 257 287 287 586 # # TauI: # [GCSGC] # 6 6 431 1372 # # TfiI: # [GAWTC] # 180 190 570 875 # # TseI: # [GCWGC] # 6 6 6 219 255 431 # 892 # # TsoI: # [TARCCA] # 27 76 210 321 485 696 # # Tsp45I: # [GTSAC] # 170 305 499 841 # # Tsp4CI*: # [ACNGT] # 59 99 99 160 161 240 # 246 272 479 # # TspDTI: # [ATGAA] # 4 20 27 64 98 127 # 188 215 419 653 # # TspEI: # [AATT] # 15 20 20 23 38 48 # 72 80 109 112 137 141 # 144 197 206 214 239 # # TspGWI: # [ACGGA] # 171 1644 # # TspRI: # [CASTGNN] # 421 1394 # # XcmI: # [CCANNNNNNNNNTGG] # 567 1248 # # XhoII: # [RGATCY] # 422 1393 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1815 # Reported_sequences: 1 # Reported_hitcount: 436 #---------------------------------------