Restriction Fragments for SMF1/YOL122C



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The currently selected gene/sequence is :

SMF1/YOL122C on chromosome XV from coordinates 91419 to 89692.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.28247.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 7 + HphI GGTGA 15 14 . . 4 9 + Hpy166II GTNNAC 6 6 . . 7 12 + AclI AACGTT 8 10 . . 8 11 + TaiI ACGT 11 7 . . 8 11 + SetI ASST 11 7 . . 8 11 + MaeII ACGT 8 10 . . 13 17 + BmgT120I GGNCC 14 15 . . 13 17 + AvaII GGWCC 13 16 . . 13 17 + AsuI* GGNCC 13 16 . . 16 20 + Hin4II* CCTTC 26 25 . . 22 25 + FatI CATG 21 25 . . 22 25 + NlaIII CATG 25 21 . . 22 25 + CviAII CATG 22 24 . . 25 35 + MwoI GCNNNNNNNGC 31 28 . . 25 29 + BlsI GCNGC 27 26 . . 25 29 + BisI GCNGC 26 27 . . 25 29 + TseI GCWGC 25 28 . . 26 31 + PstI CTGCAG 30 26 . . 26 31 + SfeI* CTRYAG 26 30 . . 27 30 + CviRI* TGCA 28 28 . . 25 29 - BbvI GCAGC 12 16 . . 29 37 + AlwNI CAGNNNCTG 34 31 . . 34 44 + MwoI GCNNNNNNNGC 40 37 . . 39 43 + FokI GGATG 52 56 . . 39 43 + BseGI GGATG 45 43 . . 43 53 + MwoI GCNNNNNNNGC 49 46 . . 43 48 + Cac8I GCNNGC 45 45 . . 43 48 + BmtI GCTAGC 47 43 . . 43 48 + NheI GCTAGC 43 47 . . 40 44 - SfaNI GCATC 30 34 . . 44 47 + MaeI CTAG 44 46 . . 47 57 + MwoI GCNNNNNNNGC 53 50 . . 51 54 + CviJI RGCY 52 52 . . 52 57 + Cac8I GCNNGC 54 54 . . 53 57 + FauI CCCGC 61 63 . . 54 57 + AciI CCGC 54 56 . . 62 71 + XmnI GAANNNNTTC 66 66 . . 64 69 + SspI AATATT 66 66 . . 70 74 + Hpy188I TCNGA 72 71 . . 73 77 + MboII GAAGA 85 84 . . 82 87 + AsuII TTCGAA 83 85 . . 83 86 + TaqI TCGA 83 85 . . 88 92 + DdeI CTNAG 88 91 . . 88 92 - BspCNI CTCAG 80 78 . . 88 92 - BseMII CTCAG 79 77 . . 90 93 - MnlI CCTC 83 82 . . 103 107 + TfiI GAWTC 103 106 . . 103 107 + HinfI GANTC 103 106 . . 107 112 + SfeI* CTRYAG 107 111 . . 109 113 + Tsp4CI* ACNGT 111 110 . . 110 116 + TspRI CASTGNN 116 107 . . 117 120 + TspEI AATT 116 120 . . 121 124 - MnlI CCTC 114 113 . . 124 128 + HphI GGTGA 136 135 . . 129 132 + CviJI RGCY 130 130 . . 131 136 + SecI* CCNNGG 131 135 . . 132 136 + BssKI CCNGG 131 136 . . 132 136 + ScrFI CCNGG 133 134 . . 132 136 + CauII* CCSGG 133 134 . . 133 136 + HpaII CCGG 133 135 . . 149 153 - BsrI ACTGG 149 147 . . 154 157 + SetI ASST 157 153 . . 154 157 + CviJI RGCY 155 155 . . 154 157 + AluI AGCT 155 155 . . 158 161 + MaeI CTAG 158 160 . . 161 165 + MaeIII GTNAC 160 165 . . 162 167 + TsoI TARCCA 178 176 . . 166 169 + FatI CATG 165 169 . . 166 169 + NlaIII CATG 169 165 . . 166 169 + CviAII CATG 166 168 . . 167 171 + TspDTI ATGAA 182 180 . . 175 178 - MnlI CCTC 168 167 . . 180 185 + SnaBI TACGTA 182 182 . . 180 185 + BsaAI YACGTR 182 182 . . 181 184 + TaiI ACGT 184 180 . . 181 184 + SetI ASST 184 180 . . 181 184 + MaeII ACGT 181 183 . . 177 182 - BciVI GTATCC 171 170 . . 178 188 - BaeI ACNNNNGTAYC 203 198 170 165 200 203 + SetI ASST 203 199 . . 198 203 - BspMI ACCTGC 189 193 . . 235 239 - TspDTI ATGAA 225 223 . . 241 245 + BmgT120I GGNCC 242 243 . . 241 245 + AvaII GGWCC 241 244 . . 241 245 + AsuI* GGNCC 241 244 . . 243 246 + SetI ASST 246 242 . . 244 248 + BssKI CCNGG 243 248 . . 244 248 + BseBI CCWGG 245 246 . . 244 248 + ScrFI CCNGG 245 246 . . 244 248 + EcoRII CCWGG 243 248 . . 252 256 - BccI CCATC 246 247 . . 254 259 - TsoI TARCCA 244 242 . . 264 267 + CviJI RGCY 265 265 . . 271 276 + ClaI ATCGAT 272 274 . . 272 275 + TaqI TCGA 272 274 . . 274 277 + DpnI GATC 275 275 . . 274 277 + BstKTI GATC 276 274 . . 274 277 + MboI GATC 273 277 . . 277 281 + BssKI CCNGG 276 281 . . 277 281 + ScrFI CCNGG 278 279 . . 277 281 + CauII* CCSGG 278 279 . . 278 281 + HpaII CCGG 278 280 . . 274 278 - BinI* GGATC 268 269 . . 283 286 + TspEI AATT 282 286 . . 295 305 + MwoI GCNNNNNNNGC 301 298 . . 297 301 + Hpy99I CGWCG 301 296 . . 299 302 + TaqI TCGA 299 301 . . 295 299 - BceAI ACGGC 280 282 . . 300 311 + BcgI CGANNNNNNTGC 289 287 323 321 303 306 + CviRI* TGCA 304 304 . . 301 305 - SfaNI GCATC 291 295 . . 306 309 + SetI ASST 309 305 . . 307 312 + NlaIV GGNNCC 309 309 . . 307 312 + HgiCI* GGYRCC 307 311 . . 304 309 - BspMI ACCTGC 295 299 . . 304 310 - AarI CACCTGC 295 299 . . 311 314 + MnlI CCTC 321 320 . . 320 323 + TspEI AATT 319 323 . . 371 374 + CviRI* TGCA 372 372 . . 372 377 + BsrDI GCAATG 379 377 . . 394 399 + NlaIV GGNNCC 396 396 . . 400 404 + MaeIII GTNAC 399 404 . . 397 401 - TspGWI ACGGA 387 385 . . 405 409 + BslFI GGGAC 419 423 . . 409 412 + MaeI CTAG 409 411 . . 411 416 + XhoII RGATCY 411 415 . . 411 416 + BglII AGATCT 411 415 . . 412 415 + DpnI GATC 413 413 . . 412 415 + BstKTI GATC 414 412 . . 412 415 + MboI GATC 411 415 . . 415 419 + DdeI CTNAG 415 418 . . 417 427 + FalI AAGNNNNNCTT 408 403 440 435 420 423 + TaqI TCGA 420 422 . . 423 426 + SetI ASST 426 422 . . 423 426 + CviJI RGCY 424 424 . . 423 426 + AluI AGCT 424 424 . . 424 429 + Cac8I GCNNGC 426 426 . . 427 430 + CviRI* TGCA 428 428 . . 417 427 - FalI AAGNNNNNCTT 440 435 408 403 442 447 + DsaI* CCRYGG 442 446 . . 442 447 + SecI* CCNNGG 442 446 . . 444 448 + Tsp4CI* ACNGT 446 445 . . 449 452 + CviJI RGCY 450 450 . . 455 459 + BsrI ACTGG 460 458 . . 474 477 + CviRI* TGCA 475 475 . . 475 485 + MwoI GCNNNNNNNGC 481 478 . . 475 485 + BstAPI GCANNNNNTGC 481 478 . . 484 494 + MwoI GCNNNNNNNGC 490 487 . . 484 488 - BceAI ACGGC 469 471 . . 491 496 + TsoI TARCCA 507 505 . . 492 495 + CviJI RGCY 493 493 . . 504 507 + SetI ASST 507 503 . . 504 507 + CviJI RGCY 505 505 . . 504 507 + AluI AGCT 505 505 . . 506 511 + Eco57MI CTGRAG 527 525 . . 506 511 + Eco57I CTGAAG 527 525 . . 518 521 + Csp6I GTAC 518 520 . . 518 521 + RsaI GTAC 519 519 . . 539 544 + Hpy178III* TCNNGA 540 542 . . 542 547 + BclI TGATCA 542 546 . . 543 546 + DpnI GATC 544 544 . . 543 546 + BstKTI GATC 545 543 . . 543 546 + MboI GATC 542 546 . . 548 558 + FalI AAGNNNNNCTT 539 534 571 566 550 555 + SduI GDGCHC 554 550 . . 550 555 + BseSI GKGCMC 554 550 . . 555 559 + Hin4II* CCTTC 565 564 . . 555 565 + BsiYI* CCNNNNNNNGG 561 558 . . 548 558 - FalI AAGNNNNNCTT 571 566 539 534 560 565 + NspBII* CMGCKG 562 562 . . 562 572 + MwoI GCNNNNNNNGC 568 565 . . 562 567 + Cac8I GCNNGC 564 564 . . 562 565 - AciI CCGC 562 564 . . 562 566 - FauI CCCGC 555 557 . . 569 574 + BalI TGGCCA 571 571 . . 569 574 + CfrI YGGCCR 569 573 . . 570 573 + CviJI RGCY 571 571 . . 570 573 + HaeIII GGCC 571 571 . . 572 586 + XcmI CCANNNNNNNNNTGG 579 578 . . 577 581 + Tsp4CI* ACNGT 579 578 . . 585 589 + FokI GGATG 598 602 . . 585 589 + BseGI GGATG 591 589 . . 603 608 + Hpy166II GTNNAC 605 605 . . 615 618 + SetI ASST 618 614 . . 615 621 + SexAI ACCWGGT 615 620 . . 616 620 + BssKI CCNGG 615 620 . . 616 620 + BseBI CCWGG 617 618 . . 616 620 + ScrFI CCNGG 617 618 . . 616 620 + EcoRII CCWGG 615 620 . . 622 626 - HgaI GACGC 611 616 . . 630 633 + TspEI AATT 629 633 . . 634 637 + SetI ASST 637 633 . . 637 641 - TspDTI ATGAA 627 625 . . 645 650 + SspI AATATT 647 647 . . 663 666 + CviRI* TGCA 664 664 . . 685 688 + CviRI* TGCA 686 686 . . 686 697 - BcgI CGANNNNNNTGC 709 707 675 673 704 707 + TspEI AATT 703 707 . . 708 711 + CviJI RGCY 709 709 . . 716 721 + Hpy178III* TCNNGA 717 719 . . 720 724 + MboII GAAGA 732 731 . . 725 728 + Csp6I GTAC 725 727 . . 720 725 - Ksp632I* CTCTTC 715 718 . . 725 728 + RsaI GTAC 726 726 . . 727 730 + TaiI ACGT 730 726 . . 727 730 + SetI ASST 730 726 . . 727 730 + MaeII ACGT 727 729 . . 730 734 - TspGWI ACGGA 720 718 . . 734 737 + MseI TTAA 734 736 . . 746 750 + Hpy188I TCNGA 748 747 . . 749 752 - MnlI CCTC 742 741 . . 760 764 + BccI CCATC 768 769 . . 797 800 + AciI CCGC 797 799 . . 806 810 + BccI CCATC 814 815 . . 810 814 + DdeI CTNAG 810 813 . . 813 816 + SetI ASST 816 812 . . 820 824 + Tsp4CI* ACNGT 822 821 . . 829 840 - CspCI CAANNNNNGTGG 853 851 818 816 847 852 + NlaIV GGNNCC 849 849 . . 851 854 + AciI CCGC 851 853 . . 859 864 + BsgI GTGCAG 880 878 . . 860 863 + CviRI* TGCA 861 861 . . 861 871 + MwoI GCNNNNNNNGC 867 864 . . 861 865 + BlsI GCNGC 863 862 . . 861 865 + BisI GCNGC 862 863 . . 861 865 + BbvI GCAGC 873 877 . . 861 865 + TseI GCWGC 861 864 . . 863 866 + CviJI RGCY 864 864 . . 865 870 + StyI CCWWGG 865 869 . . 865 870 + SecI* CCNNGG 865 869 . . 869 872 + CviJI RGCY 870 870 . . 884 887 + TaiI ACGT 887 883 . . 884 887 + SetI ASST 887 883 . . 884 887 + MaeII ACGT 884 886 . . 887 890 + MseI TTAA 887 889 . . 893 897 + Tsp4CI* ACNGT 895 894 . . 898 901 + TspEI AATT 897 901 . . 904 908 + Tsp4CI* ACNGT 906 905 . . 910 914 + Hpy188I TCNGA 912 911 . . 911 916 + Eco57MI CTGRAG 932 930 . . 911 916 + Eco57I CTGAAG 932 930 . . 913 917 + MboII GAAGA 925 924 . . 945 951 + TspRI CASTGNN 951 942 . . 947 952 + Eco57MI CTGRAG 968 966 . . 947 952 + Eco57I CTGAAG 968 966 . . 949 953 + MboII GAAGA 961 960 . . 949 954 - Ksp632I* CTCTTC 944 947 . . 961 965 + MboII GAAGA 973 972 . . 968 973 + SspI AATATT 970 970 . . 974 977 + MseI TTAA 974 976 . . 976 979 + TspEI AATT 975 979 . . 994 998 + BlsI GCNGC 996 995 . . 994 998 + BisI GCNGC 995 996 . . 994 998 + TseI GCWGC 994 997 . . 994 998 - BbvI GCAGC 981 985 . . 1004 1009 + SspI AATATT 1006 1006 . . 1009 1012 + CviRI* TGCA 1010 1010 . . 1011 1014 + FatI CATG 1010 1014 . . 1011 1014 + NlaIII CATG 1014 1010 . . 1011 1014 + CviAII CATG 1011 1013 . . 1012 1016 + TspDTI ATGAA 1027 1025 . . 1016 1021 + SspI AATATT 1018 1018 . . 1028 1031 + TaqI TCGA 1028 1030 . . 1031 1034 + TspEI AATT 1030 1034 . . 1033 1036 + MseI TTAA 1033 1035 . . 1045 1050 + Ksp632I* CTCTTC 1051 1054 . . 1046 1050 - MboII GAAGA 1038 1037 . . 1049 1053 - HphI GGTGA 1041 1040 . . 1054 1057 + MaeI CTAG 1054 1056 . . 1056 1061 + HaeII RGCGCY 1060 1056 . . 1056 1061 + Eco47III AGCGCT 1058 1058 . . 1057 1060 + Hin6I GCGC 1057 1059 . . 1057 1060 + GlaI GCGC 1058 1058 . . 1057 1060 + HhaI GCGC 1059 1057 . . 1068 1073 + MfeI CAATTG 1068 1072 . . 1069 1072 + TspEI AATT 1068 1072 . . 1076 1080 + BccI CCATC 1084 1085 . . 1077 1081 - FokI GGATG 1063 1067 . . 1077 1081 - BseGI GGATG 1076 1074 . . 1081 1084 + MaeI CTAG 1081 1083 . . 1090 1095 + Cac8I GCNNGC 1092 1092 . . 1092 1097 + SduI GDGCHC 1096 1092 . . 1092 1097 + HgiJII* GRGCYC 1096 1092 . . 1093 1096 + CviJI RGCY 1094 1094 . . 1093 1098 + NlaIV GGNNCC 1095 1095 . . 1090 1093 - AciI CCGC 1090 1092 . . 1090 1094 - FauI CCCGC 1083 1085 . . 1111 1115 - HphI GGTGA 1103 1102 . . 1120 1130 + MwoI GCNNNNNNNGC 1126 1123 . . 1123 1127 - BccI CCATC 1117 1118 . . 1137 1142 + Hpy166II GTNNAC 1139 1139 . . 1146 1151 + Hpy178III* TCNNGA 1147 1149 . . 1146 1151 + BspHI TCATGA 1146 1150 . . 1147 1150 + FatI CATG 1146 1150 . . 1147 1150 + NlaIII CATG 1150 1146 . . 1147 1150 + CviAII CATG 1147 1149 . . 1148 1152 + TspDTI ATGAA 1163 1161 . . 1145 1149 - TspDTI ATGAA 1135 1133 . . 1151 1154 + TspEI AATT 1150 1154 . . 1159 1164 + Hpy178III* TCNNGA 1160 1162 . . 1170 1175 + NlaIV GGNNCC 1172 1172 . . 1170 1175 + HgiCI* GGYRCC 1170 1174 . . 1171 1181 + MwoI GCNNNNNNNGC 1177 1174 . . 1174 1178 + FauI CCCGC 1182 1184 . . 1174 1184 + BsiYI* CCNNNNNNNGG 1180 1177 . . 1175 1178 + AciI CCGC 1175 1177 . . 1177 1181 + BlsI GCNGC 1179 1178 . . 1177 1181 + BisI GCNGC 1178 1179 . . 1177 1181 + BbvI GCAGC 1189 1193 . . 1177 1181 + TseI GCWGC 1177 1180 . . 1178 1183 + EcoP15I CAGCAG 1208 1210 . . 1182 1185 + SetI ASST 1185 1181 . . 1184 1187 + Csp6I GTAC 1184 1186 . . 1184 1187 + RsaI GTAC 1185 1185 . . 1180 1185 - BspMI ACCTGC 1171 1175 . . 1187 1198 + BcgI CGANNNNNNTGC 1176 1174 1210 1208 1194 1197 + FatI CATG 1193 1197 . . 1194 1197 + NlaIII CATG 1197 1193 . . 1194 1197 + CviAII CATG 1194 1196 . . 1192 1196 - TspDTI ATGAA 1182 1180 . . 1197 1202 + Cac8I GCNNGC 1199 1199 . . 1210 1213 + MseI TTAA 1210 1212 . . 1223 1226 + AciI CCGC 1223 1225 . . 1227 1230 + SetI ASST 1230 1226 . . 1225 1230 - BspMI ACCTGC 1216 1220 . . 1225 1231 - AarI CACCTGC 1216 1220 . . 1236 1241 + Hpy166II GTNNAC 1238 1238 . . 1237 1242 + TatI WGTACW 1237 1241 . . 1238 1241 + Csp6I GTAC 1238 1240 . . 1238 1241 + RsaI GTAC 1239 1239 . . 1254 1257 + TspEI AATT 1253 1257 . . 1264 1267 - MnlI CCTC 1257 1256 . . 1273 1278 + VspI ATTAAT 1274 1276 . . 1274 1277 + MseI TTAA 1274 1276 . . 1276 1279 + TspEI AATT 1275 1279 . . 1286 1289 + CviRI* TGCA 1287 1287 . . 1287 1297 + MwoI GCNNNNNNNGC 1293 1290 . . 1287 1291 + BlsI GCNGC 1289 1288 . . 1287 1291 + BisI GCNGC 1288 1289 . . 1287 1291 + BbvI GCAGC 1299 1303 . . 1287 1291 + TseI GCWGC 1287 1290 . . 1289 1292 + CviJI RGCY 1290 1290 . . 1291 1296 + DsaI* CCRYGG 1291 1295 . . 1291 1296 + StyI CCWWGG 1291 1295 . . 1291 1296 + NcoI CCATGG 1291 1295 . . 1291 1296 + SecI* CCNNGG 1291 1295 . . 1292 1295 + FatI CATG 1291 1295 . . 1292 1295 + NlaIII CATG 1295 1291 . . 1292 1295 + CviAII CATG 1292 1294 . . 1301 1305 + MboII GAAGA 1313 1312 . . 1307 1312 + BalI TGGCCA 1309 1309 . . 1307 1312 + CfrI YGGCCR 1307 1311 . . 1308 1311 + CviJI RGCY 1309 1309 . . 1308 1311 + HaeIII GGCC 1309 1309 . . 1313 1316 + MaeI CTAG 1313 1315 . . 1324 1327 + TaqI TCGA 1324 1326 . . 1332 1342 + BsiYI* CCNNNNNNNGG 1338 1335 . . 1367 1372 + HindIII AAGCTT 1367 1371 . . 1368 1371 + SetI ASST 1371 1367 . . 1368 1371 + CviJI RGCY 1369 1369 . . 1368 1371 + AluI AGCT 1369 1369 . . 1379 1384 + StuI AGGCCT 1381 1381 . . 1380 1383 + CviJI RGCY 1381 1381 . . 1380 1383 + HaeIII GGCC 1381 1381 . . 1381 1391 + MwoI GCNNNNNNNGC 1387 1384 . . 1384 1387 + MseI TTAA 1384 1386 . . 1434 1437 + SetI ASST 1437 1433 . . 1438 1441 + MseI TTAA 1438 1440 . . 1440 1443 + TspEI AATT 1439 1443 . . 1445 1449 - MboII GAAGA 1437 1436 . . 1450 1455 + BspLU11I* ACATGT 1450 1454 . . 1450 1455 + NspI RCATGY 1454 1450 . . 1450 1455 + AflIII ACRYGT 1450 1454 . . 1451 1454 + FatI CATG 1450 1454 . . 1451 1454 + NlaIII CATG 1454 1450 . . 1451 1454 + CviAII CATG 1451 1453 . . 1466 1471 + Hpy178III* TCNNGA 1467 1469 . . 1466 1471 + BspHI TCATGA 1466 1470 . . 1467 1470 + FatI CATG 1466 1470 . . 1467 1470 + NlaIII CATG 1470 1466 . . 1467 1470 + CviAII CATG 1467 1469 . . 1468 1472 + TspDTI ATGAA 1483 1481 . . 1479 1482 + TspEI AATT 1478 1482 . . 1483 1487 + Tsp4CI* ACNGT 1485 1484 . . 1485 1489 + Hpy99I CGWCG 1489 1484 . . 1486 1491 + SalI GTCGAC 1486 1490 . . 1486 1491 + Hpy166II GTNNAC 1488 1488 . . 1486 1491 + HindII GTYRAC 1488 1488 . . 1486 1491 + AccI GTMKAC 1487 1489 . . 1487 1490 + TaqI TCGA 1487 1489 . . 1496 1501 + Eco57MI CTGRAG 1517 1515 . . 1496 1501 + Eco57I CTGAAG 1517 1515 . . 1498 1502 + MboII GAAGA 1510 1509 . . 1501 1505 + MboII GAAGA 1513 1512 . . 1506 1511 + TsoI TARCCA 1522 1520 . . 1507 1510 + CviJI RGCY 1508 1508 . . 1512 1517 + TsoI TARCCA 1528 1526 . . 1515 1519 + BccI CCATC 1523 1524 . . 1530 1541 + BcgI CGANNNNNNTGC 1519 1517 1553 1551 1534 1539 + XhoII RGATCY 1534 1538 . . 1534 1539 + BglII AGATCT 1534 1538 . . 1535 1538 + DpnI GATC 1536 1536 . . 1535 1538 + BstKTI GATC 1537 1535 . . 1535 1538 + MboI GATC 1534 1538 . . 1538 1543 + PstI CTGCAG 1542 1538 . . 1538 1543 + SfeI* CTRYAG 1538 1542 . . 1539 1542 + CviRI* TGCA 1540 1540 . . 1542 1545 + SetI ASST 1545 1541 . . 1540 1545 - BspMI ACCTGC 1531 1535 . . 1553 1556 + TaqI TCGA 1553 1555 . . 1561 1565 - BccI CCATC 1555 1556 . . 1568 1571 + MaeI CTAG 1568 1570 . . 1575 1578 + FatI CATG 1574 1578 . . 1575 1578 + NlaIII CATG 1578 1574 . . 1575 1578 + CviAII CATG 1575 1577 . . 1623 1628 + MfeI CAATTG 1623 1627 . . 1624 1627 + TspEI AATT 1623 1627 . . 1635 1641 + TspRI CASTGNN 1641 1632 . . 1636 1640 + Tsp4CI* ACNGT 1638 1637 . . 1650 1653 + TspEI AATT 1649 1653 . . 1658 1661 + CviJI RGCY 1659 1659 . . 1678 1683 + AclI AACGTT 1679 1681 . . 1679 1682 + TaiI ACGT 1682 1678 . . 1679 1682 + SetI ASST 1682 1678 . . 1679 1682 + MaeII ACGT 1679 1681 . . 1697 1700 + TspEI AATT 1696 1700 . . 1703 1708 + NspI RCATGY 1707 1703 . . 1704 1707 + FatI CATG 1703 1707 . . 1704 1707 + NlaIII CATG 1707 1703 . . 1704 1707 + CviAII CATG 1704 1706 . . 1707 1711 + BsmAI GTCTC 1712 1716 . . 1711 1714 + FatI CATG 1710 1714 . . 1711 1714 + NlaIII CATG 1714 1710 . . 1711 1714 + CviAII CATG 1711 1713 . . 1714 1718 + HphI GGTGA 1726 1725 . . 1717 1722 + EcoRV GATATC 1719 1719 . . 1725 1728 + MseI TTAA 1725 1727 . . #--------------------------------------- # # # AarI: # [CACCTGC] # 295 512 921 # # AccI: # [GTMKAC] # 241 1487 # # AciI: # [CCGC] # 48 54 54 85 235 239 # 505 508 # # AclI: # [AACGTT] # 8 49 1671 # # AflIII: # [ACRYGT] # 278 1450 # # AluI: # [AGCT] # 81 155 269 359 864 # # AlwNI: # [CAGNNNCTG] # 34 1694 # # AsuI*: # [GGNCC] # 13 228 1487 # # AsuII: # [TTCGAA] # 83 1645 # # AvaII: # [GGWCC] # 13 228 1487 # # BaeI: # [ACNNNNGTAYC] # 33 170 1525 # # BalI: # [TGGCCA] # 419 571 738 # # BbvI: # [GCAGC] # 12 108 110 208 429 861 # # BccI: # [CCATC] # 32 33 46 173 246 270 # 406 522 # # BceAI: # [ACGGC] # 189 280 1259 # # BcgI: # [CGANNNNNNTGC] # 34 34 34 34 175 289 # 309 352 467 # # BciVI: # [GTATCC] # 171 1557 # # BclI: # [TGATCA] # 542 1186 # # BglII: # [AGATCT] # 194 411 1123 # # BinI*: # [GGATC] # 268 1460 # # BisI: # [GCNGC] # 26 110 133 183 440 836 # # BlsI: # [GCNGC] # 27 110 133 183 439 836 # # BmgT120I: # [GGNCC] # 14 228 1486 # # BmtI: # [GCTAGC] # 47 1681 # # BsaAI: # [YACGTR] # 182 1546 # # BseBI: # [CCWGG] # 245 372 1111 # # BseGI: # [GGATG] # 45 485 546 652 # # BseMII: # [CTCAG] # 79 1649 # # BseSI: # [GKGCMC] # 554 1174 # # BsgI: # [GTGCAG] # 848 880 # # BsiYI*: # [CCNNNNNNNGG] # 158 390 561 619 # # BslFI: # [GGGAC] # 419 1309 # # BsmAI: # [GTCTC] # 16 1712 # # BspCNI: # [CTCAG] # 80 1648 # # BspHI: # [TCATGA] # 262 320 1146 # # BspLU11I*: # [ACATGT] # 278 1450 # # BspMI: # [ACCTGC] # 45 106 189 197 315 876 # # BsrDI: # [GCAATG] # 379 1349 # # BsrI: # [ACTGG] # 149 311 1268 # # BssKI: # [CCNGG] # 33 112 131 339 1113 # # BstAPI: # [GCANNNNNTGC] # 481 1247 # # BstKTI: # [GATC] # 131 138 191 276 992 # # Cac8I: # [GCNNGC] # 9 45 107 138 372 528 # 529 # # CauII*: # [CCSGG] # 133 145 1450 # # CfrI: # [YGGCCR] # 421 569 738 # # ClaI: # [ATCGAT] # 272 1456 # # Csp6I: # [GTAC] # 54 207 459 490 518 # # CspCI: # [CAANNNNNGTGG] # 35 818 875 # # CviAII: # [CATG] # 7 16 17 22 47 98 # 108 129 136 144 159 845 # # CviJI: # [RGCY] # 6 12 12 19 25 26 # 43 52 60 66 69 78 # 110 127 138 151 155 159 # 196 224 # # CviRI*: # [TGCA] # 22 28 47 56 68 149 # 175 188 189 253 276 277 # # DdeI: # [CTNAG] # 88 327 395 918 # # DpnI: # [GATC] # 131 138 192 275 992 # # DsaI*: # [CCRYGG] # 437 442 849 # # Eco47III: # [AGCGCT] # 670 1058 # # Eco57I: # [CTGAAG] # 36 211 405 527 549 # # Eco57MI: # [CTGRAG] # 36 211 405 527 549 # # EcoP15I: # [CAGCAG] # 520 1208 # # EcoRII: # [CCWGG] # 243 372 1113 # # EcoRV: # [GATATC] # 9 1719 # # FalI: # [AAGNNNNNCTT] # 0 0 0 0 32 32 # 99 408 1157 # # FatI: # [CATG] # 7 16 18 21 47 98 # 108 129 136 144 159 845 # # FauI: # [CCCGC] # 61 99 494 528 546 # # FokI: # [GGATG] # 52 465 546 665 # # GlaI: # [GCGC] # 670 1058 # # HaeII: # [RGCGCY] # 668 1060 # # HaeIII: # [GGCC] # 72 347 571 738 # # HgaI: # [GACGC] # 611 1117 # # HgiCI*: # [GGYRCC] # 307 558 863 # # HgiJII*: # [GRGCYC] # 632 1096 # # HhaI: # [GCGC] # 669 1059 # # Hin4II*: # [CCTTC] # 26 539 1163 # # Hin6I: # [GCGC] # 671 1057 # # HindII: # [GTYRAC] # 240 1488 # # HindIII: # [AAGCTT] # 361 1367 # # HinfI: # [GANTC] # 103 1625 # # HpaII: # [CCGG] # 133 145 1450 # # HphI: # [GGTGA] # 2 15 62 121 623 905 # # Hpy166II: # [GTNNAC] # 6 99 240 250 534 599 # # Hpy178III*: # [TCNNGA] # 13 177 261 307 430 540 # # Hpy188I: # [TCNGA] # 72 164 676 816 # # Hpy99I: # [CGWCG] # 239 301 1188 # # Ksp632I*: # [CTCTTC] # 107 229 677 715 # # MaeI: # [CTAG] # 27 44 114 160 232 251 # 255 645 # # MaeII: # [ACGT] # 8 49 157 173 546 795 # # MaeIII: # [GTNAC] # 160 239 1329 # # MboI: # [GATC] # 131 138 194 273 992 # # MboII: # [GAAGA] # 3 12 36 65 73 85 # 124 193 215 275 647 # # MfeI: # [CAATTG] # 105 555 1068 # # MnlI: # [CCTC] # 31 54 83 153 421 471 # 515 # # MseI: # [TTAA] # 3 54 59 64 87 110 # 153 177 287 734 # # MwoI: # [GCNNNNNNNGC] # 4 9 9 9 31 51 # 78 94 116 180 248 259 # 299 341 # # NcoI: # [CCATGG] # 437 1291 # # NheI: # [GCTAGC] # 43 1685 # # NlaIII: # [CATG] # 7 14 16 25 47 98 # 108 129 136 144 159 845 # # NlaIV: # [GGNNCC] # 77 87 246 309 453 556 # # NspBII*: # [CMGCKG] # 562 1166 # # NspI: # [RCATGY] # 21 253 1454 # # PstI: # [CTGCAG] # 30 186 1512 # # RsaI: # [GTAC] # 54 207 459 489 519 # # SalI: # [GTCGAC] # 242 1486 # # ScrFI: # [CCNGG] # 33 112 133 339 1111 # # SduI: # [GDGCHC] # 542 554 632 # # SecI*: # [CCNNGG] # 131 311 423 426 437 # # SetI: # [ASST] # 11 19 19 27 43 45 # 46 63 66 71 81 86 # 93 108 111 117 137 141 # 146 298 # # SexAI: # [ACCWGGT] # 615 1113 # # SfaNI: # [GCATC] # 30 261 1437 # # SfeI*: # [CTRYAG] # 26 81 190 1431 # # SnaBI: # [TACGTA] # 182 1546 # # SspI: # [AATATT] # 12 36 66 323 581 710 # # StuI: # [AGGCCT] # 347 1381 # # StyI: # [CCWWGG] # 426 437 865 # # TaiI: # [ACGT] # 11 46 157 173 546 795 # # TaqI: # [TCGA] # 27 66 83 121 163 175 # 189 296 608 # # TatI: # [WGTACW] # 491 1237 # # TfiI: # [GAWTC] # 103 1625 # # TseI: # [GCWGC] # 25 110 133 183 441 836 # # TsoI: # [TARCCA] # 6 66 178 200 263 1015 # # Tsp4CI*: # [ACNGT] # 11 73 90 111 133 153 # 243 335 579 # # TspDTI: # [ATGAA] # 19 28 43 108 182 245 # 301 400 402 # # TspEI: # [AATT] # 22 26 32 37 38 39 # 47 55 74 78 82 103 # 116 145 164 166 194 310 # # TspGWI: # [ACGGA] # 333 387 1008 # # TspRI: # [CASTGNN] # 87 116 690 835 # # VspI: # [ATTAAT] # 454 1274 # # XcmI: # [CCANNNNNNNNNTGG] # 579 1149 # # XhoII: # [RGATCY] # 194 411 1123 # # XmnI: # [GAANNNNTTC] # 66 1662 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1728 # Reported_sequences: 1 # Reported_hitcount: 437 #---------------------------------------