Restriction Fragments for YOL097W-A



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The currently selected gene/sequence is :

YOL097W-A on chromosome XV from coordinates 136220 to 136405.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.23252.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 2 5 + CviRI* TGCA 3 3 . . 9 12 + FatI CATG 8 12 . . 9 12 + NlaIII CATG 12 8 . . 9 12 + CviAII CATG 9 11 . . 12 15 + DpnI GATC 13 13 . . 12 15 + BstKTI GATC 14 12 . . 12 15 + MboI GATC 11 15 . . 22 26 + Hpy188I TCNGA 24 23 . . 23 27 + DdeI CTNAG 23 26 . . 25 30 + SduI GDGCHC 29 25 . . 25 30 + HgiAI* GWGCWC 29 25 . . 23 27 - BspCNI CTCAG 15 13 . . 23 27 - BseMII CTCAG 14 12 . . 35 38 + SetI ASST 38 34 . . 40 43 + SetI ASST 43 39 . . 40 45 + BspMI ACCTGC 49 53 . . 43 46 + CviRI* TGCA 44 44 . . 47 52 + TatI WGTACW 47 51 . . 47 52 + ScaI AGTACT 49 49 . . 48 51 + Csp6I GTAC 48 50 . . 48 51 + RsaI GTAC 49 49 . . 50 54 + BsrI ACTGG 55 53 . . 53 56 + CviJI RGCY 54 54 . . 53 56 + HaeIII GGCC 54 54 . . 61 64 + SetI ASST 64 60 . . 61 64 + CviJI RGCY 62 62 . . 61 64 + AluI AGCT 62 62 . . 66 69 + MnlI CCTC 76 75 . . 82 92 - Hin4I GAYNNNNNVTC 105 100 73 68 95 106 + AlfI GCANNNNNNTGC 84 82 118 116 96 105 + MslI CAYNNNNRTG 100 100 . . 98 104 + TspRI CASTGNN 104 95 . . 104 107 + CviRI* TGCA 105 105 . . 102 106 - SfaNI GCATC 92 96 . . 95 106 - AlfI GCANNNNNNTGC 118 116 84 82 112 116 - BccI CCATC 106 107 . . 130 134 - BceAI ACGGC 115 117 . . 138 143 + ApoI RAATTY 138 142 . . 139 142 + TspEI AATT 138 142 . . 164 169 + ApoI RAATTY 164 168 . . 165 168 + TspEI AATT 164 168 . . 170 174 - TspDTI ATGAA 160 158 . . 181 186 + SfeI* CTRYAG 181 185 . . #--------------------------------------- # # # AlfI: # [GCANNNNNNTGC] # 0 0 34 68 84 # # AluI: # [AGCT] # 62 124 # # ApoI: # [RAATTY] # 22 26 138 # # BccI: # [CCATC] # 80 106 # # BceAI: # [ACGGC] # 71 115 # # BseMII: # [CTCAG] # 14 172 # # BspCNI: # [CTCAG] # 15 171 # # BspMI: # [ACCTGC] # 49 137 # # BsrI: # [ACTGG] # 55 131 # # BstKTI: # [GATC] # 14 172 # # Csp6I: # [GTAC] # 48 138 # # CviAII: # [CATG] # 9 177 # # CviJI: # [RGCY] # 8 54 124 # # CviRI*: # [TGCA] # 3 41 61 81 # # DdeI: # [CTNAG] # 23 163 # # DpnI: # [GATC] # 13 173 # # FatI: # [CATG] # 8 178 # # HaeIII: # [GGCC] # 54 132 # # HgiAI*: # [GWGCWC] # 29 157 # # Hin4I: # [GAYNNNNNVTC] # 32 73 81 # # Hpy188I: # [TCNGA] # 24 162 # # MboI: # [GATC] # 11 175 # # MnlI: # [CCTC] # 76 110 # # MslI: # [CAYNNNNRTG] # 86 100 # # NlaIII: # [CATG] # 12 174 # # RsaI: # [GTAC] # 49 137 # # ScaI: # [AGTACT] # 49 137 # # SduI: # [GDGCHC] # 29 157 # # SetI: # [ASST] # 5 21 38 122 # # SfaNI: # [GCATC] # 92 94 # # SfeI*: # [CTRYAG] # 5 181 # # TatI: # [WGTACW] # 47 139 # # TspDTI: # [ATGAA] # 26 160 # # TspEI: # [AATT] # 22 26 138 # # TspRI: # [CASTGNN] # 82 104 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 186 # Reported_sequences: 1 # Reported_hitcount: 43 #---------------------------------------