Restriction Fragments for DIS3/YOL021C



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The currently selected gene/sequence is :

DIS3/YOL021C on chromosome XV from coordinates 285426 to 282421.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.29596.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 10 14 + FauI CCCGC 18 20 . . 11 14 + AciI CCGC 11 13 . . 20 30 + BsiYI* CCNNNNNNNGG 26 23 . . 27 31 + TspGWI ACGGA 42 40 . . 30 34 + MboII GAAGA 42 41 . . 30 35 - Ksp632I* CTCTTC 25 28 . . 33 37 - BsmAI GTCTC 27 31 . . 39 42 + CviRI* TGCA 40 40 . . 43 47 - BccI CCATC 37 38 . . 49 52 + MseI TTAA 49 51 . . 55 59 + Tsp45I GTSAC 54 59 . . 55 59 + MaeIII GTNAC 54 59 . . 53 57 - HgaI GACGC 42 47 . . 59 63 + DdeI CTNAG 59 62 . . 59 63 + BspCNI CTCAG 72 70 . . 59 63 + BseMII CTCAG 73 71 . . 60 64 + Hpy188I TCNGA 62 61 . . 65 68 + SetI ASST 68 64 . . 63 67 - Hin4II* CCTTC 57 56 . . 76 79 + SetI ASST 79 75 . . 77 82 + Eco31I GGTCTC 83 87 . . 78 82 + BsmAI GTCTC 83 87 . . 91 96 + HaeII RGCGCY 95 91 . . 89 92 - MnlI CCTC 82 81 . . 92 95 + Hin6I GCGC 92 94 . . 92 95 + GlaI GCGC 93 93 . . 92 95 + HhaI GCGC 94 92 . . 102 105 + DpnI GATC 103 103 . . 102 105 + BstKTI GATC 104 102 . . 102 105 + MboI GATC 101 105 . . 121 124 + MseI TTAA 121 123 . . 125 135 + Hin4I GAYNNNNNVTC 116 111 148 143 125 128 + DpnI GATC 126 126 . . 125 128 + BstKTI GATC 127 125 . . 125 128 + MboI GATC 124 128 . . 127 131 + Hpy188I TCNGA 129 128 . . 125 135 - Hin4I GAYNNNNNVTC 148 143 116 111 132 136 - FokI GGATG 118 122 . . 132 136 - BseGI GGATG 131 129 . . 137 140 + FatI CATG 136 140 . . 137 140 + NlaIII CATG 140 136 . . 137 140 + CviAII CATG 137 139 . . 145 148 + TaqI TCGA 145 147 . . 145 150 + Hpy178III* TCNNGA 146 148 . . 155 158 + Csp6I GTAC 155 157 . . 155 158 + RsaI GTAC 156 156 . . 166 169 + AciI CCGC 166 168 . . 171 174 + TspEI AATT 170 174 . . 178 182 - BslFI GGGAC 163 167 . . 184 188 - SfaNI GCATC 174 178 . . 205 215 + FalI AAGNNNNNCTT 196 191 228 223 208 212 - TspDTI ATGAA 198 196 . . 205 215 - FalI AAGNNNNNCTT 228 223 196 191 217 221 + Hpy188I TCNGA 219 218 . . 220 224 + TfiI GAWTC 220 223 . . 220 224 + HinfI GANTC 220 223 . . 228 232 + DdeI CTNAG 228 231 . . 233 236 + TspEI AATT 232 236 . . 235 238 + MseI TTAA 235 237 . . 239 242 + Hin6I GCGC 239 241 . . 239 242 + GlaI GCGC 240 240 . . 239 242 + HhaI GCGC 241 239 . . 242 247 + TaqII CACCCA 258 256 . . 260 263 + TaiI ACGT 263 259 . . 260 263 + SetI ASST 263 259 . . 260 263 + MaeII ACGT 260 262 . . 261 265 + Hpy99I CGWCG 265 260 . . 270 275 - BciVI GTATCC 264 263 . . 278 281 + TaiI ACGT 281 277 . . 278 281 + SetI ASST 281 277 . . 278 281 + MaeII ACGT 278 280 . . 271 281 - BaeI ACNNNNGTAYC 296 291 263 258 285 289 + MaeIII GTNAC 284 289 . . 293 298 + MfeI CAATTG 293 297 . . 294 297 + TspEI AATT 293 297 . . 299 302 + SetI ASST 302 298 . . 309 313 + TfiI GAWTC 309 312 . . 309 313 + HinfI GANTC 309 312 . . 312 316 + Hpy188I TCNGA 314 313 . . 316 319 + TspEI AATT 315 319 . . 326 329 + TaqI TCGA 326 328 . . 327 331 + Hpy99I CGWCG 331 326 . . 328 333 + ZraI GACGTC 330 330 . . 328 333 + AcyI GRCGYC 329 331 . . 328 333 + AatII GACGTC 332 328 . . 328 339 + DrdI GACNNNNNNGTC 334 332 . . 329 332 + TaiI ACGT 332 328 . . 329 332 + SetI ASST 332 328 . . 329 332 + MaeII ACGT 329 331 . . 337 341 - BslFI GGGAC 322 326 . . 352 355 + MaeI CTAG 352 354 . . 356 360 + TspDTI ATGAA 371 369 . . 378 383 + BetI* WCCGGW 378 382 . . 379 382 + HpaII CCGG 379 381 . . 382 387 + Hpy166II GTNNAC 384 384 . . 383 388 + TatI WGTACW 383 387 . . 383 388 + Bsp1407I TGTACA 383 387 . . 384 389 + Hpy166II GTNNAC 386 386 . . 384 387 + Csp6I GTAC 384 386 . . 384 387 + RsaI GTAC 385 385 . . 387 392 + BsiI* CACGAG 387 391 . . 394 397 + MseI TTAA 394 396 . . 406 409 + CviRI* TGCA 407 407 . . 416 420 + Tsp45I GTSAC 415 420 . . 416 420 + MaeIII GTNAC 415 420 . . 421 424 + DpnI GATC 422 422 . . 421 424 + BstKTI GATC 423 421 . . 421 424 + MboI GATC 420 424 . . 431 435 + TfiI GAWTC 431 434 . . 431 435 + HinfI GANTC 431 434 . . 433 437 - TspDTI ATGAA 423 421 . . 443 447 - TspDTI ATGAA 433 431 . . 451 456 + ApoI RAATTY 451 455 . . 452 455 + TspEI AATT 451 455 . . 458 463 + Hpy166II GTNNAC 460 460 . . 475 484 + XmnI GAANNNNTTC 479 479 . . 479 482 + CviJI RGCY 480 480 . . 488 492 + TspDTI ATGAA 503 501 . . 496 501 + VspI ATTAAT 497 499 . . 497 500 + MseI TTAA 497 499 . . 516 519 + CviJI RGCY 517 517 . . 529 532 + SetI ASST 532 528 . . 530 540 + BsiYI* CCNNNNNNNGG 536 533 . . 547 552 + SduI GDGCHC 551 547 . . 547 552 + HgiAI* GWGCWC 551 547 . . 558 561 + SetI ASST 561 557 . . 565 576 + DrdI GACNNNNNNGTC 571 569 . . 572 575 + TaiI ACGT 575 571 . . 572 575 + SetI ASST 575 571 . . 572 575 + MaeII ACGT 572 574 . . 573 577 + Hpy99I CGWCG 577 572 . . 583 587 + MaeIII GTNAC 582 587 . . 591 596 + McrI* CGRYCG 594 592 . . 591 596 + PvuI CGATCG 594 592 . . 592 595 + DpnI GATC 593 593 . . 592 595 + BstKTI GATC 594 592 . . 592 595 + MboI GATC 591 595 . . 609 612 + SetI ASST 612 608 . . 609 612 + CviJI RGCY 610 610 . . 609 612 + AluI AGCT 610 610 . . 610 614 + BlsI GCNGC 612 611 . . 610 614 + BisI GCNGC 611 612 . . 610 614 + TseI GCWGC 610 613 . . 614 628 + XcmI CCANNNNNNNNNTGG 621 620 . . 610 614 - BbvI GCAGC 597 601 . . 617 621 + DdeI CTNAG 617 620 . . 628 632 + PleI GAGTC 636 637 . . 628 632 + MlyI GAGTC 637 637 . . 628 632 + HinfI GANTC 628 631 . . 641 646 + TsoI TARCCA 657 655 . . 644 655 + BstXI CCANNNNNNTGG 651 647 . . 649 652 + SetI ASST 652 648 . . 649 652 + CviJI RGCY 650 650 . . 649 652 + AluI AGCT 650 650 . . 655 658 + Csp6I GTAC 655 657 . . 655 658 + RsaI GTAC 656 656 . . 657 661 + Tsp4CI* ACNGT 659 658 . . 665 668 + TaqI TCGA 665 667 . . 669 673 + MaeIII GTNAC 668 673 . . 681 684 + CviRI* TGCA 682 682 . . 692 696 + Hpy188I TCNGA 694 693 . . 697 701 + HinfI GANTC 697 700 . . 695 699 - BsmAI GTCTC 689 693 . . 697 701 - PleI GAGTC 691 692 . . 697 701 - MlyI GAGTC 691 691 . . 706 709 + MnlI CCTC 716 715 . . 715 719 + TfiI GAWTC 715 718 . . 715 719 + HinfI GANTC 715 718 . . 724 733 + BsaBI GATNNNNATC 728 728 . . 729 734 + XhoII RGATCY 729 733 . . 729 734 + BglII AGATCT 729 733 . . 730 733 + DpnI GATC 731 731 . . 730 733 + BstKTI GATC 732 730 . . 730 733 + MboI GATC 729 733 . . 732 737 + Hpy178III* TCNNGA 733 735 . . 740 744 + BslFI GGGAC 754 758 . . 751 755 + Hpy188I TCNGA 753 752 . . 760 763 + TaiI ACGT 763 759 . . 760 763 + SetI ASST 763 759 . . 760 763 + MaeII ACGT 760 762 . . 764 770 + PfoI TCCNGGA 764 769 . . 765 769 + BssKI CCNGG 764 769 . . 765 769 + ScrFI CCNGG 766 767 . . 765 769 + CauII* CCSGG 766 767 . . 766 769 + HpaII CCGG 766 768 . . 770 775 + TatI WGTACW 770 774 . . 770 775 + ScaI AGTACT 772 772 . . 771 774 + Csp6I GTAC 771 773 . . 771 774 + RsaI GTAC 772 772 . . 779 784 + SfeI* CTRYAG 779 783 . . 794 799 - TaqII CACCCA 784 782 . . 819 822 + Csp6I GTAC 819 821 . . 819 822 + RsaI GTAC 820 820 . . 822 826 + BssKI CCNGG 821 826 . . 822 826 + BseBI CCWGG 823 824 . . 822 827 + SecI* CCNNGG 822 826 . . 822 826 + ScrFI CCNGG 823 824 . . 822 826 + EcoRII CCWGG 821 826 . . 829 834 + SspI AATATT 831 831 . . 841 845 + Hpy188I TCNGA 843 842 . . 845 850 + TatI WGTACW 845 849 . . 846 849 + Csp6I GTAC 846 848 . . 846 849 + RsaI GTAC 847 847 . . 855 860 + XbaI TCTAGA 855 859 . . 855 860 + Hpy178III* TCNNGA 856 858 . . 856 859 + MaeI CTAG 856 858 . . 861 864 + SetI ASST 864 860 . . 859 863 - Hin4II* CCTTC 853 852 . . 866 872 + TspRI CASTGNN 872 863 . . 872 877 + Hpy166II GTNNAC 874 874 . . 893 899 + TspRI CASTGNN 899 890 . . 892 896 - BsrI ACTGG 892 890 . . 898 901 + MseI TTAA 898 900 . . 900 903 + TspEI AATT 899 903 . . 917 920 + SetI ASST 920 916 . . 928 933 - BsmI GAATGC 928 926 . . 935 939 + Tsp4CI* ACNGT 937 936 . . 937 943 + BstEII GGTNACC 937 942 . . 937 941 + HphI GGTGA 949 948 . . 938 942 + Tsp45I GTSAC 937 942 . . 938 942 + MaeIII GTNAC 937 942 . . 941 947 + SexAI ACCWGGT 941 946 . . 942 946 + BssKI CCNGG 941 946 . . 942 946 + BseBI CCWGG 943 944 . . 942 946 + ScrFI CCNGG 943 944 . . 942 946 + EcoRII CCWGG 941 946 . . 944 947 + SetI ASST 947 943 . . 963 966 + SetI ASST 966 962 . . 964 967 + MnlI CCTC 974 973 . . 970 974 + Hpy188I TCNGA 972 971 . . 981 986 + NlaIV GGNNCC 983 983 . . 981 986 + HgiCI* GGYRCC 981 985 . . 978 982 - Hin4II* CCTTC 972 971 . . 986 989 + MnlI CCTC 996 995 . . 992 1003 + BstXI CCANNNNNNTGG 999 995 . . 989 993 - FokI GGATG 975 979 . . 989 993 - BseGI GGATG 988 986 . . 1003 1007 + TfiI GAWTC 1003 1006 . . 1003 1007 + HinfI GANTC 1003 1006 . . 1006 1010 + Hpy188I TCNGA 1008 1007 . . 1016 1019 + TaqI TCGA 1016 1018 . . 1017 1021 + Hpy99I CGWCG 1021 1016 . . 1019 1022 + TaiI ACGT 1022 1018 . . 1019 1022 + SetI ASST 1022 1018 . . 1019 1022 + MaeII ACGT 1019 1021 . . 1021 1026 + Hpy166II GTNNAC 1023 1023 . . 1021 1026 + HpaI GTTAAC 1023 1023 . . 1021 1026 + HindII GTYRAC 1023 1023 . . 1022 1025 + MseI TTAA 1022 1024 . . 1044 1047 + CviJI RGCY 1045 1045 . . 1042 1045 - MnlI CCTC 1035 1034 . . 1045 1050 + Cac8I GCNNGC 1047 1047 . . 1049 1054 + BtgZI GCGATG 1064 1068 . . 1069 1073 + PleI GAGTC 1077 1078 . . 1069 1073 + MlyI GAGTC 1078 1078 . . 1069 1073 + HinfI GANTC 1069 1072 . . 1073 1076 + MnlI CCTC 1083 1082 . . 1074 1079 + Ksp632I* CTCTTC 1080 1083 . . 1075 1079 - MboII GAAGA 1067 1066 . . 1087 1091 + Tsp4CI* ACNGT 1089 1088 . . 1096 1101 + MmeI TCCRAC 1121 1119 . . 1096 1100 + Hpy188I TCNGA 1098 1097 . . 1104 1114 + MwoI GCNNNNNNNGC 1110 1107 . . 1104 1108 + BlsI GCNGC 1106 1105 . . 1104 1108 + BisI GCNGC 1105 1106 . . 1104 1108 + BbvI GCAGC 1116 1120 . . 1104 1108 + TseI GCWGC 1104 1107 . . 1107 1110 + Hin6I GCGC 1107 1109 . . 1107 1110 + GlaI GCGC 1108 1108 . . 1107 1117 + MwoI GCNNNNNNNGC 1113 1110 . . 1107 1110 + HhaI GCGC 1109 1107 . . 1109 1114 + Cac8I GCNNGC 1111 1111 . . 1112 1115 + CviJI RGCY 1113 1113 . . 1113 1117 + BlsI GCNGC 1115 1114 . . 1113 1117 + BisI GCNGC 1114 1115 . . 1113 1117 + TseI GCWGC 1113 1116 . . 1116 1121 + Cac8I GCNNGC 1118 1118 . . 1113 1117 - BbvI GCAGC 1100 1104 . . 1120 1130 + MwoI GCNNNNNNNGC 1126 1123 . . 1129 1139 + MwoI GCNNNNNNNGC 1135 1132 . . 1126 1130 - SfaNI GCATC 1116 1120 . . 1137 1140 + SetI ASST 1140 1136 . . 1137 1140 + CviJI RGCY 1138 1138 . . 1137 1140 + AluI AGCT 1138 1138 . . 1145 1148 + DpnI GATC 1146 1146 . . 1145 1148 + BstKTI GATC 1147 1145 . . 1145 1148 + MboI GATC 1144 1148 . . 1147 1150 + TaqI TCGA 1147 1149 . . 1154 1159 + ApoI RAATTY 1154 1158 . . 1155 1158 + TspEI AATT 1154 1158 . . 1160 1163 + CviJI RGCY 1161 1161 . . 1163 1168 + DsaI* CCRYGG 1163 1167 . . 1163 1168 + SecI* CCNNGG 1163 1167 . . 1165 1169 + BceAI ACGGC 1181 1183 . . 1190 1194 + MboII GAAGA 1202 1201 . . 1192 1197 + XhoII RGATCY 1192 1196 . . 1193 1196 + DpnI GATC 1194 1194 . . 1193 1196 + BstKTI GATC 1195 1193 . . 1193 1196 + MboI GATC 1192 1196 . . 1195 1201 + PfoI TCCNGGA 1195 1200 . . 1196 1200 + BssKI CCNGG 1195 1200 . . 1196 1200 + BseBI CCWGG 1197 1198 . . 1196 1200 + ScrFI CCNGG 1197 1198 . . 1196 1200 + EcoRII CCWGG 1195 1200 . . 1197 1202 + Eco57MI CTGRAG 1218 1216 . . 1197 1202 + GsuI CTGGAG 1218 1216 . . 1193 1197 - BinI* GGATC 1187 1188 . . 1202 1212 + Hin4I GAYNNNNNVTC 1193 1188 1225 1220 1202 1212 + Eam1105I GACNNNNNGTC 1207 1206 . . 1203 1207 + Tsp4CI* ACNGT 1205 1204 . . 1200 1204 - BsmAI GTCTC 1194 1198 . . 1206 1209 + Csp6I GTAC 1206 1208 . . 1206 1209 + RsaI GTAC 1207 1207 . . 1208 1211 + TaiI ACGT 1211 1207 . . 1208 1211 + SetI ASST 1211 1207 . . 1208 1211 + MaeII ACGT 1208 1210 . . 1209 1213 + Hpy99I CGWCG 1213 1208 . . 1202 1212 - Hin4I GAYNNNNNVTC 1225 1220 1193 1188 1214 1219 + Hpy166II GTNNAC 1216 1216 . . 1214 1219 + HindII GTYRAC 1216 1216 . . 1211 1216 - TaqII GACCGA 1201 1199 . . 1219 1222 + MaeI CTAG 1219 1221 . . 1237 1240 + DpnI GATC 1238 1238 . . 1237 1240 + BstKTI GATC 1239 1237 . . 1237 1240 + MboI GATC 1236 1240 . . 1237 1241 - BinI* GGATC 1231 1232 . . 1252 1257 + TatI WGTACW 1252 1256 . . 1253 1258 + Hpy166II GTNNAC 1255 1255 . . 1253 1256 + Csp6I GTAC 1253 1255 . . 1253 1256 + RsaI GTAC 1254 1254 . . 1260 1269 + XmnI GAANNNNTTC 1264 1264 . . 1265 1269 - MboII GAAGA 1257 1256 . . 1264 1269 - BbvII* GAAGAC 1257 1261 . . 1282 1291 + PshAI GACNNNNGTC 1286 1286 . . 1278 1282 - BccI CCATC 1272 1273 . . 1290 1294 - MboII GAAGA 1282 1281 . . 1289 1294 - BbvII* GAAGAC 1282 1286 . . 1301 1305 + Hpy188I TCNGA 1303 1302 . . 1304 1308 + TfiI GAWTC 1304 1307 . . 1304 1308 + HinfI GANTC 1304 1307 . . 1307 1311 + Hpy188I TCNGA 1309 1308 . . 1317 1322 + Cac8I GCNNGC 1319 1319 . . 1321 1325 + BlsI GCNGC 1323 1322 . . 1321 1325 + BisI GCNGC 1322 1323 . . 1321 1325 + TseI GCWGC 1321 1324 . . 1321 1325 - BbvI GCAGC 1308 1312 . . 1322 1327 - EcoP15I CAGCAG 1294 1296 . . 1328 1331 + TspEI AATT 1327 1331 . . 1341 1344 - MnlI CCTC 1334 1333 . . 1360 1364 + TfiI GAWTC 1360 1363 . . 1360 1364 + HinfI GANTC 1360 1363 . . 1367 1370 + CviJI RGCY 1368 1368 . . 1367 1370 + HaeIII GGCC 1368 1368 . . 1367 1371 + BmgT120I GGNCC 1368 1369 . . 1367 1371 + AsuI* GGNCC 1367 1370 . . 1392 1395 + SetI ASST 1395 1391 . . 1394 1398 + Tsp45I GTSAC 1393 1398 . . 1394 1398 + MaeIII GTNAC 1393 1398 . . 1396 1404 + DraIII CACNNNGTG 1401 1398 . . 1409 1412 + SetI ASST 1412 1408 . . 1410 1413 + MnlI CCTC 1420 1419 . . 1410 1415 + SecI* CCNNGG 1410 1414 . . 1406 1410 - BsmAI GTCTC 1400 1404 . . 1406 1411 - Eco31I GGTCTC 1400 1404 . . 1415 1418 + Csp6I GTAC 1415 1417 . . 1415 1418 + RsaI GTAC 1416 1416 . . 1418 1423 + MfeI CAATTG 1418 1422 . . 1419 1422 + TspEI AATT 1418 1422 . . 1422 1433 + BdaI TGANNNNNNTCA 1411 1409 1445 1443 1423 1427 + TfiI GAWTC 1423 1426 . . 1423 1427 + HinfI GANTC 1423 1426 . . 1430 1435 + SmlI CTYRAG 1430 1434 . . 1422 1433 - BdaI TGANNNNNNTCA 1445 1443 1411 1409 1434 1437 + SetI ASST 1437 1433 . . 1434 1437 + CviJI RGCY 1435 1435 . . 1434 1437 + AluI AGCT 1435 1435 . . 1430 1435 - Bce83I* CTTGAG 1415 1413 . . 1445 1450 + StuI AGGCCT 1447 1447 . . 1446 1449 + CviJI RGCY 1447 1447 . . 1446 1449 + HaeIII GGCC 1447 1447 . . 1444 1447 - MnlI CCTC 1437 1436 . . 1455 1460 - MmeI TCCRAC 1436 1434 . . 1462 1465 + FatI CATG 1461 1465 . . 1462 1465 + NlaIII CATG 1465 1461 . . 1462 1465 + CviAII CATG 1462 1464 . . 1477 1482 + StuI AGGCCT 1479 1479 . . 1478 1481 + CviJI RGCY 1479 1479 . . 1478 1481 + HaeIII GGCC 1479 1479 . . 1497 1502 + XbaI TCTAGA 1497 1501 . . 1497 1502 + Hpy178III* TCNNGA 1498 1500 . . 1498 1501 + MaeI CTAG 1498 1500 . . 1509 1514 + Cac8I GCNNGC 1511 1511 . . 1510 1514 + FauI CCCGC 1518 1520 . . 1510 1520 + BsiYI* CCNNNNNNNGG 1516 1513 . . 1511 1514 + AciI CCGC 1511 1513 . . 1519 1522 + CviJI RGCY 1520 1520 . . 1519 1522 + HaeIII GGCC 1520 1520 . . 1516 1520 - Hin4II* CCTTC 1510 1509 . . 1532 1538 + DraII RGGNCCY 1533 1536 . . 1533 1536 + CviJI RGCY 1534 1534 . . 1533 1536 + HaeIII GGCC 1534 1534 . . 1533 1538 + NlaIV GGNNCC 1535 1535 . . 1533 1537 + BmgT120I GGNCC 1534 1535 . . 1533 1537 + AsuI* GGNCC 1533 1536 . . 1530 1534 - Hin4II* CCTTC 1524 1523 . . 1545 1549 + BsrI ACTGG 1550 1548 . . 1552 1555 + DpnI GATC 1553 1553 . . 1552 1555 + BstKTI GATC 1554 1552 . . 1552 1555 + MboI GATC 1551 1555 . . 1554 1559 + Hpy178III* TCNNGA 1555 1557 . . 1555 1561 + SauI* CCTNAGG 1556 1559 . . 1556 1560 + DdeI CTNAG 1556 1559 . . 1552 1556 - BinI* GGATC 1546 1547 . . 1560 1563 + CviJI RGCY 1561 1561 . . 1556 1560 - BspCNI CTCAG 1548 1546 . . 1556 1560 - BseMII CTCAG 1547 1545 . . 1558 1561 - MnlI CCTC 1551 1550 . . 1572 1577 + XhoII RGATCY 1572 1576 . . 1572 1577 + NlaIV GGNNCC 1574 1574 . . 1572 1577 + BamHI GGATCC 1572 1576 . . 1572 1576 + BinI* GGATC 1580 1581 . . 1573 1576 + DpnI GATC 1574 1574 . . 1573 1576 + BstKTI GATC 1575 1573 . . 1573 1576 + MboI GATC 1572 1576 . . 1573 1577 - BinI* GGATC 1567 1568 . . 1596 1601 + XhoII RGATCY 1596 1600 . . 1596 1600 + BinI* GGATC 1604 1605 . . 1597 1600 + DpnI GATC 1598 1598 . . 1597 1600 + BstKTI GATC 1599 1597 . . 1597 1600 + MboI GATC 1596 1600 . . 1600 1604 + DdeI CTNAG 1600 1603 . . 1600 1604 + BspCNI CTCAG 1613 1611 . . 1600 1604 + BseMII CTCAG 1614 1612 . . 1601 1605 + Hpy188I TCNGA 1603 1602 . . 1624 1629 + ClaI ATCGAT 1625 1627 . . 1625 1628 + TaqI TCGA 1625 1627 . . 1627 1630 + DpnI GATC 1628 1628 . . 1627 1630 + BstKTI GATC 1629 1627 . . 1627 1630 + MboI GATC 1626 1630 . . 1630 1633 + MnlI CCTC 1640 1639 . . 1627 1631 - BinI* GGATC 1621 1622 . . 1632 1638 + PfoI TCCNGGA 1632 1637 . . 1633 1637 + BssKI CCNGG 1632 1637 . . 1633 1637 + BseBI CCWGG 1634 1635 . . 1633 1637 + ScrFI CCNGG 1634 1635 . . 1633 1637 + EcoRII CCWGG 1632 1637 . . 1636 1640 + FokI GGATG 1649 1653 . . 1631 1636 - Eco57MI CTGRAG 1616 1614 . . 1636 1640 + BseGI GGATG 1642 1640 . . 1631 1636 - GsuI CTGGAG 1616 1614 . . 1657 1667 + MwoI GCNNNNNNNGC 1663 1660 . . 1654 1658 - SfaNI GCATC 1644 1648 . . 1662 1667 + NspI RCATGY 1666 1662 . . 1663 1666 + FatI CATG 1662 1666 . . 1663 1666 + NlaIII CATG 1666 1662 . . 1663 1666 + CviAII CATG 1663 1665 . . 1672 1677 + HindIII AAGCTT 1672 1676 . . 1673 1676 + SetI ASST 1676 1672 . . 1673 1676 + CviJI RGCY 1674 1674 . . 1673 1676 + AluI AGCT 1674 1674 . . 1678 1692 + XcmI CCANNNNNNNNNTGG 1685 1684 . . 1682 1686 + Tsp4CI* ACNGT 1684 1683 . . 1687 1690 + TspEI AATT 1686 1690 . . 1703 1707 - TspDTI ATGAA 1693 1691 . . 1713 1724 + BdaI TGANNNNNNTCA 1702 1700 1736 1734 1717 1721 + MaeIII GTNAC 1716 1721 . . 1713 1724 - BdaI TGANNNNNNTCA 1736 1734 1702 1700 1730 1733 + MseI TTAA 1730 1732 . . 1735 1739 + BssKI CCNGG 1734 1739 . . 1735 1738 + HpaII CCGG 1735 1737 . . 1735 1739 + ScrFI CCNGG 1736 1737 . . 1735 1739 + CauII* CCSGG 1736 1737 . . 1737 1742 + SduI GDGCHC 1741 1737 . . 1737 1742 + BseSI GKGCMC 1741 1737 . . 1740 1746 + TspRI CASTGNN 1746 1737 . . 1744 1754 + MwoI GCNNNNNNNGC 1750 1747 . . 1740 1745 - BtsI GCAGTG 1739 1737 . . 1745 1750 + SecI* CCNNGG 1745 1749 . . 1746 1750 + BssKI CCNGG 1745 1750 . . 1746 1750 + BseBI CCWGG 1747 1748 . . 1746 1750 + ScrFI CCNGG 1747 1748 . . 1746 1750 + EcoRII CCWGG 1745 1750 . . 1746 1756 + BsiYI* CCNNNNNNNGG 1752 1749 . . 1749 1753 + FokI GGATG 1762 1766 . . 1749 1753 + BseGI GGATG 1755 1753 . . 1753 1763 + MwoI GCNNNNNNNGC 1759 1756 . . 1750 1754 - SfaNI GCATC 1740 1744 . . 1753 1756 - AciI CCGC 1753 1755 . . 1758 1761 + SetI ASST 1761 1757 . . 1756 1760 - Hin4II* CCTTC 1750 1749 . . 1762 1766 + BlsI GCNGC 1764 1763 . . 1762 1766 + BisI GCNGC 1763 1764 . . 1762 1766 + TseI GCWGC 1762 1765 . . 1764 1767 + CviRI* TGCA 1765 1765 . . 1762 1766 - BbvI GCAGC 1749 1753 . . 1767 1777 + FalI AAGNNNNNCTT 1758 1753 1790 1785 1770 1773 + SetI ASST 1773 1769 . . 1769 1772 - MnlI CCTC 1762 1761 . . 1772 1775 + Csp6I GTAC 1772 1774 . . 1772 1775 + RsaI GTAC 1773 1773 . . 1767 1777 - FalI AAGNNNNNCTT 1790 1785 1758 1753 1771 1781 - BaeI ACNNNNGTAYC 1796 1791 1763 1758 1789 1794 + Hpy166II GTNNAC 1791 1791 . . 1789 1794 + AccI GTMKAC 1790 1792 . . 1797 1800 + TaiI ACGT 1800 1796 . . 1797 1800 + SetI ASST 1800 1796 . . 1797 1800 + MaeII ACGT 1797 1799 . . 1805 1810 + NspI RCATGY 1809 1805 . . 1806 1809 + FatI CATG 1805 1809 . . 1806 1809 + NlaIII CATG 1809 1805 . . 1806 1809 + CviAII CATG 1806 1808 . . 1809 1819 + MwoI GCNNNNNNNGC 1815 1812 . . 1809 1813 + BlsI GCNGC 1811 1810 . . 1809 1813 + BisI GCNGC 1810 1811 . . 1809 1813 + TseI GCWGC 1809 1812 . . 1809 1813 - BbvI GCAGC 1796 1800 . . 1815 1818 + FatI CATG 1814 1818 . . 1815 1818 + NlaIII CATG 1818 1814 . . 1815 1818 + CviAII CATG 1815 1817 . . 1822 1825 + MaeI CTAG 1822 1824 . . 1824 1827 + SetI ASST 1827 1823 . . 1825 1830 + KpnI GGTACC 1829 1825 . . 1825 1830 + NlaIV GGNNCC 1827 1827 . . 1825 1830 + HgiCI* GGYRCC 1825 1829 . . 1825 1830 + Acc65I GGTACC 1825 1829 . . 1826 1829 + Csp6I GTAC 1826 1828 . . 1826 1829 + RsaI GTAC 1827 1827 . . 1832 1835 + SetI ASST 1835 1831 . . 1853 1856 + TaiI ACGT 1856 1852 . . 1853 1856 + SetI ASST 1856 1852 . . 1853 1856 + MaeII ACGT 1853 1855 . . 1862 1866 + TfiI GAWTC 1862 1865 . . 1862 1866 + HinfI GANTC 1862 1865 . . 1867 1872 - BsmI GAATGC 1867 1865 . . 1886 1889 + TspEI AATT 1885 1889 . . 1890 1894 + FokI GGATG 1903 1907 . . 1890 1894 + BseGI GGATG 1896 1894 . . 1903 1908 + SspI AATATT 1905 1905 . . 1916 1919 + MseI TTAA 1916 1918 . . 1918 1921 + TspEI AATT 1917 1921 . . 1924 1928 + TspDTI ATGAA 1939 1937 . . 1925 1936 + BdaI TGANNNNNNTCA 1914 1912 1948 1946 1930 1934 - TspGWI ACGGA 1920 1918 . . 1925 1936 - BdaI TGANNNNNNTCA 1948 1946 1914 1912 1937 1941 + Hpy188I TCNGA 1939 1938 . . 1939 1944 + XhoII RGATCY 1939 1943 . . 1939 1944 + BglII AGATCT 1939 1943 . . 1940 1943 + DpnI GATC 1941 1941 . . 1940 1943 + BstKTI GATC 1942 1940 . . 1940 1943 + MboI GATC 1939 1943 . . 1942 1947 + XbaI TCTAGA 1942 1946 . . 1942 1947 + Hpy178III* TCNNGA 1943 1945 . . 1943 1946 + MaeI CTAG 1943 1945 . . 1949 1954 + HindIII AAGCTT 1949 1953 . . 1950 1953 + SetI ASST 1953 1949 . . 1950 1953 + CviJI RGCY 1951 1951 . . 1950 1953 + AluI AGCT 1951 1951 . . 1975 1979 + DdeI CTNAG 1975 1978 . . 1975 1979 - BspCNI CTCAG 1967 1965 . . 1975 1979 - BseMII CTCAG 1966 1964 . . 1980 1983 + TspEI AATT 1979 1983 . . 2003 2007 + TspDTI ATGAA 2018 2016 . . 2006 2009 + TspEI AATT 2005 2009 . . 2008 2011 + MseI TTAA 2008 2010 . . 2013 2017 - BccI CCATC 2007 2008 . . 2024 2029 + SduI GDGCHC 2028 2024 . . 2024 2029 + HgiJII* GRGCYC 2028 2024 . . 2022 2025 - MnlI CCTC 2015 2014 . . 2025 2028 + CviJI RGCY 2026 2026 . . 2030 2035 + Hpy178III* TCNNGA 2031 2033 . . 2036 2039 + TspEI AATT 2035 2039 . . 2050 2055 + Eco57MI CTGRAG 2071 2069 . . 2050 2055 + Eco57I CTGAAG 2071 2069 . . 2065 2068 + MaeI CTAG 2065 2067 . . 2061 2065 - BsmAI GTCTC 2055 2059 . . 2068 2071 - MnlI CCTC 2061 2060 . . 2073 2076 + SetI ASST 2076 2072 . . 2074 2079 + NlaIV GGNNCC 2076 2076 . . 2074 2079 + HgiCI* GGYRCC 2074 2078 . . 2071 2076 - BspMI ACCTGC 2062 2066 . . 2071 2077 - AarI CACCTGC 2062 2066 . . 2085 2089 + DdeI CTNAG 2085 2088 . . 2088 2091 + SetI ASST 2091 2087 . . 2088 2091 + CviJI RGCY 2089 2089 . . 2088 2091 + AluI AGCT 2089 2089 . . 2094 2099 + Hpy178III* TCNNGA 2095 2097 . . 2095 2101 + SauI* CCTNAGG 2096 2099 . . 2096 2100 + DdeI CTNAG 2096 2099 . . 2099 2102 + SetI ASST 2102 2098 . . 2096 2100 - BspCNI CTCAG 2088 2086 . . 2096 2100 - BseMII CTCAG 2087 2085 . . 2098 2101 - MnlI CCTC 2091 2090 . . 2102 2105 + MseI TTAA 2102 2104 . . 2105 2108 + SetI ASST 2108 2104 . . 2106 2110 + BmgT120I GGNCC 2107 2108 . . 2106 2110 + AvaII GGWCC 2106 2109 . . 2106 2110 + AsuI* GGNCC 2106 2109 . . 2110 2115 + NdeI CATATG 2111 2113 . . 2124 2134 + Hin4I GAYNNNNNVTC 2115 2110 2147 2142 2122 2126 - BsmAI GTCTC 2116 2120 . . 2128 2132 + Hpy188I TCNGA 2130 2129 . . 2130 2135 + XhoII RGATCY 2130 2134 . . 2126 2131 - Eco57MI CTGRAG 2111 2109 . . 2131 2134 + DpnI GATC 2132 2132 . . 2131 2134 + BstKTI GATC 2133 2131 . . 2131 2134 + MboI GATC 2130 2134 . . 2126 2131 - Eco57I CTGAAG 2111 2109 . . 2124 2134 - Hin4I GAYNNNNNVTC 2147 2142 2115 2110 2135 2146 + CspCI CAANNNNNGTGG 2123 2121 2158 2156 2131 2135 - BinI* GGATC 2125 2126 . . 2138 2142 + TspDTI ATGAA 2153 2151 . . 2156 2159 + TspEI AATT 2155 2159 . . 2160 2164 + BsrI ACTGG 2165 2163 . . 2169 2174 + ApoI RAATTY 2169 2173 . . 2170 2173 + TspEI AATT 2169 2173 . . 2182 2186 + MboII GAAGA 2194 2193 . . 2185 2190 + ApoI RAATTY 2185 2189 . . 2186 2189 + TspEI AATT 2185 2189 . . 2199 2202 + CviJI RGCY 2200 2200 . . 2198 2203 - TsoI TARCCA 2188 2186 . . 2210 2216 + TspRI CASTGNN 2216 2207 . . 2220 2224 + MboII GAAGA 2232 2231 . . 2230 2234 - SfaNI GCATC 2220 2224 . . 2234 2238 + Hin4II* CCTTC 2244 2243 . . 2238 2248 + BsiYI* CCNNNNNNNGG 2244 2241 . . 2239 2242 + MnlI CCTC 2249 2248 . . 2245 2249 + BceAI ACGGC 2261 2263 . . 2248 2253 + BtgZI GCGATG 2263 2267 . . 2250 2254 - SfaNI GCATC 2240 2244 . . 2254 2258 + DdeI CTNAG 2254 2257 . . 2258 2262 + MboII GAAGA 2270 2269 . . 2258 2263 + BbvII* GAAGAC 2265 2269 . . 2266 2270 + BlsI GCNGC 2268 2267 . . 2266 2270 + BisI GCNGC 2267 2268 . . 2266 2270 + BbvI GCAGC 2278 2282 . . 2266 2270 + TseI GCWGC 2266 2269 . . 2268 2271 + SetI ASST 2271 2267 . . 2268 2271 + CviJI RGCY 2269 2269 . . 2268 2271 + AluI AGCT 2269 2269 . . 2272 2275 + AciI CCGC 2272 2274 . . 2275 2279 + BccI CCATC 2283 2284 . . 2271 2276 - EciI GGCGGA 2261 2259 . . 2284 2287 + TspEI AATT 2283 2287 . . 2296 2299 + MseI TTAA 2296 2298 . . 2300 2304 + TspDTI ATGAA 2315 2313 . . 2308 2311 + MseI TTAA 2308 2310 . . 2331 2334 + TspEI AATT 2330 2334 . . 2341 2345 + TfiI GAWTC 2341 2344 . . 2341 2345 + HinfI GANTC 2341 2344 . . 2337 2342 - MmeI TCCRAC 2318 2316 . . 2348 2359 + BstXI CCANNNNNNTGG 2355 2351 . . 2348 2353 + StyI CCWWGG 2348 2352 . . 2348 2353 + SecI* CCNNGG 2348 2352 . . 2351 2356 + StuI AGGCCT 2353 2353 . . 2352 2355 + CviJI RGCY 2353 2353 . . 2352 2355 + HaeIII GGCC 2353 2353 . . 2354 2359 + StyI CCWWGG 2354 2358 . . 2354 2359 + SecI* CCNNGG 2354 2358 . . 2357 2362 + CfrI YGGCCR 2357 2361 . . 2358 2361 + CviJI RGCY 2359 2359 . . 2358 2361 + HaeIII GGCC 2359 2359 . . 2362 2366 + TfiI GAWTC 2362 2365 . . 2362 2366 + HinfI GANTC 2362 2365 . . 2374 2377 + SetI ASST 2377 2373 . . 2382 2387 + XhoII RGATCY 2382 2386 . . 2382 2387 + NlaIV GGNNCC 2384 2384 . . 2382 2387 + BamHI GGATCC 2382 2386 . . 2382 2386 + BinI* GGATC 2390 2391 . . 2383 2386 + DpnI GATC 2384 2384 . . 2383 2386 + BstKTI GATC 2385 2383 . . 2383 2386 + MboI GATC 2382 2386 . . 2385 2390 + Hpy178III* TCNNGA 2386 2388 . . 2383 2387 - BinI* GGATC 2377 2378 . . 2389 2393 + MboII GAAGA 2401 2400 . . 2391 2396 + XhoII RGATCY 2391 2395 . . 2392 2395 + DpnI GATC 2393 2393 . . 2392 2395 + BstKTI GATC 2394 2392 . . 2392 2395 + MboI GATC 2391 2395 . . 2392 2396 - BinI* GGATC 2386 2387 . . 2402 2405 + MseI TTAA 2402 2404 . . 2421 2424 + FatI CATG 2420 2424 . . 2421 2424 + NlaIII CATG 2424 2420 . . 2421 2424 + CviAII CATG 2421 2423 . . 2424 2429 + SalI GTCGAC 2424 2428 . . 2424 2429 + Hpy166II GTNNAC 2426 2426 . . 2424 2429 + HindII GTYRAC 2426 2426 . . 2424 2429 + AccI GTMKAC 2425 2427 . . 2425 2428 + TaqI TCGA 2425 2427 . . 2427 2431 + HinfI GANTC 2427 2430 . . 2427 2431 - PleI GAGTC 2421 2422 . . 2427 2431 - MlyI GAGTC 2421 2421 . . 2443 2447 + BlsI GCNGC 2445 2444 . . 2443 2447 + BisI GCNGC 2444 2445 . . 2439 2443 - BccI CCATC 2433 2434 . . 2443 2447 + BbvI GCAGC 2455 2459 . . 2443 2447 + TseI GCWGC 2443 2446 . . 2445 2448 + CviJI RGCY 2446 2446 . . 2461 2466 + Hpy178III* TCNNGA 2462 2464 . . 2462 2467 + Eco57MI CTGRAG 2483 2481 . . 2462 2467 + GsuI CTGGAG 2483 2481 . . 2466 2469 + SetI ASST 2469 2465 . . 2466 2469 + CviJI RGCY 2467 2467 . . 2466 2469 + AluI AGCT 2467 2467 . . 2478 2483 + Hpy178III* TCNNGA 2479 2481 . . 2502 2505 + CviJI RGCY 2503 2503 . . 2503 2507 - BceAI ACGGC 2488 2490 . . 2509 2514 + EcoRV GATATC 2511 2511 . . 2532 2535 + SetI ASST 2535 2531 . . 2530 2534 - HphI GGTGA 2522 2521 . . 2541 2544 + TaiI ACGT 2544 2540 . . 2541 2544 + SetI ASST 2544 2540 . . 2541 2544 + MaeII ACGT 2541 2543 . . 2543 2547 + MaeIII GTNAC 2542 2547 . . 2546 2550 + Tsp4CI* ACNGT 2548 2547 . . 2559 2562 + CviJI RGCY 2560 2560 . . 2559 2562 + HaeIII GGCC 2560 2560 . . 2559 2563 + BmgT120I GGNCC 2560 2561 . . 2559 2563 + AsuI* GGNCC 2559 2562 . . 2570 2573 + TspEI AATT 2569 2573 . . 2577 2580 + SetI ASST 2580 2576 . . 2578 2583 + NlaIV GGNNCC 2580 2580 . . 2578 2583 + HgiCI* GGYRCC 2578 2582 . . 2575 2580 - BspMI ACCTGC 2566 2570 . . 2575 2581 - AarI CACCTGC 2566 2570 . . 2591 2595 + TspDTI ATGAA 2606 2604 . . 2599 2603 + DdeI CTNAG 2599 2602 . . 2607 2611 + HinfI GANTC 2607 2610 . . 2607 2611 - PleI GAGTC 2601 2602 . . 2607 2611 - MlyI GAGTC 2601 2601 . . 2613 2618 + Hpy178III* TCNNGA 2614 2616 . . 2634 2637 + FatI CATG 2633 2637 . . 2634 2637 + NlaIII CATG 2637 2633 . . 2634 2637 + CviAII CATG 2634 2636 . . 2641 2644 + CviRI* TGCA 2642 2642 . . 2671 2681 + MwoI GCNNNNNNNGC 2677 2674 . . 2675 2678 + TspEI AATT 2674 2678 . . 2680 2690 + MwoI GCNNNNNNNGC 2686 2683 . . 2680 2685 + Cfr10I RCCGGY 2680 2684 . . 2680 2690 + BglI GCCNNNNNGGC 2686 2683 . . 2681 2684 + HpaII CCGG 2681 2683 . . 2689 2694 + Cac8I GCNNGC 2691 2691 . . 2721 2724 - MnlI CCTC 2714 2713 . . 2731 2735 + PleI GAGTC 2739 2740 . . 2731 2735 + MlyI GAGTC 2740 2740 . . 2731 2735 + HinfI GANTC 2731 2734 . . 2733 2738 + Hpy166II GTNNAC 2735 2735 . . 2738 2746 + AlwNI CAGNNNCTG 2743 2740 . . 2743 2747 + BsrI ACTGG 2748 2746 . . 2756 2759 + MseI TTAA 2756 2758 . . 2759 2762 + SetI ASST 2762 2758 . . 2765 2768 + MseI TTAA 2765 2767 . . 2782 2785 + Csp6I GTAC 2782 2784 . . 2782 2785 + RsaI GTAC 2783 2783 . . 2784 2788 + BsrI ACTGG 2789 2787 . . 2787 2792 + NlaIV GGNNCC 2789 2789 . . 2791 2801 + BsiYI* CCNNNNNNNGG 2797 2794 . . 2792 2806 + XcmI CCANNNNNNNNNTGG 2799 2798 . . 2801 2811 + MwoI GCNNNNNNNGC 2807 2804 . . 2809 2812 + CviJI RGCY 2810 2810 . . 2806 2810 - Hin4II* CCTTC 2800 2799 . . 2820 2824 + BsrI ACTGG 2825 2823 . . 2827 2830 + TspEI AATT 2826 2830 . . 2832 2842 + Hin4I GAYNNNNNVTC 2823 2818 2855 2850 2834 2838 + DdeI CTNAG 2834 2837 . . 2838 2843 + XhoII RGATCY 2838 2842 . . 2834 2838 - BspCNI CTCAG 2826 2824 . . 2834 2838 - BseMII CTCAG 2825 2823 . . 2838 2843 + NlaIV GGNNCC 2840 2840 . . 2838 2843 + BamHI GGATCC 2838 2842 . . 2838 2842 + BinI* GGATC 2846 2847 . . 2836 2839 - MnlI CCTC 2829 2828 . . 2839 2849 + Hin4I GAYNNNNNVTC 2830 2825 2862 2857 2839 2842 + DpnI GATC 2840 2840 . . 2839 2842 + BstKTI GATC 2841 2839 . . 2839 2842 + MboI GATC 2838 2842 . . 2832 2842 - Hin4I GAYNNNNNVTC 2855 2850 2823 2818 2839 2843 - BinI* GGATC 2833 2834 . . 2847 2851 + DdeI CTNAG 2847 2850 . . 2847 2851 + BspCNI CTCAG 2860 2858 . . 2847 2851 + BseMII CTCAG 2861 2859 . . 2850 2853 + CviJI RGCY 2851 2851 . . 2851 2855 + TauI GCSGC 2854 2851 . . 2851 2855 + BlsI GCNGC 2853 2852 . . 2851 2855 + BisI GCNGC 2852 2853 . . 2852 2855 + AciI CCGC 2852 2854 . . 2864 2867 + SetI ASST 2867 2863 . . 2863 2866 - MnlI CCTC 2856 2855 . . 2876 2879 + TspEI AATT 2875 2879 . . 2878 2881 + MseI TTAA 2878 2880 . . 2881 2884 + SetI ASST 2884 2880 . . 2895 2900 + ApoI RAATTY 2895 2899 . . 2896 2899 + TspEI AATT 2895 2899 . . 2899 2903 + Hpy188I TCNGA 2901 2900 . . 2908 2913 + SecI* CCNNGG 2908 2912 . . 2910 2913 - MnlI CCTC 2903 2902 . . 2913 2917 + FokI GGATG 2926 2930 . . 2913 2917 + BseGI GGATG 2919 2917 . . 2917 2922 + Hpy166II GTNNAC 2919 2919 . . 2921 2924 + TaiI ACGT 2924 2920 . . 2921 2924 + SetI ASST 2924 2920 . . 2921 2924 + MaeII ACGT 2921 2923 . . 2927 2930 + TaqI TCGA 2927 2929 . . 2933 2936 + SetI ASST 2936 2932 . . 2936 2939 + TaqI TCGA 2936 2938 . . 2950 2953 + SetI ASST 2953 2949 . . 2955 2959 + HphI GGTGA 2967 2966 . . 2961 2966 + XhoII RGATCY 2961 2965 . . 2961 2966 + NlaIV GGNNCC 2963 2963 . . 2961 2966 + BamHI GGATCC 2961 2965 . . 2961 2965 + BinI* GGATC 2969 2970 . . 2962 2965 + DpnI GATC 2963 2963 . . 2962 2965 + BstKTI GATC 2964 2962 . . 2962 2965 + MboI GATC 2961 2965 . . 2958 2962 - BccI CCATC 2952 2953 . . 2962 2966 - BinI* GGATC 2956 2957 . . 2967 2970 + TspEI AATT 2966 2970 . . 2972 2975 + MaeI CTAG 2972 2974 . . 2975 2980 + Cac8I GCNNGC 2977 2977 . . 2990 2993 + TspEI AATT 2989 2993 . . 2998 3001 + MseI TTAA 2998 3000 . . #--------------------------------------- # # # AarI: # [CACCTGC] # 440 504 2062 # # AatII: # [GACGTC] # 332 2674 # # Acc65I: # [GGTACC] # 1181 1825 # # AccI: # [GTMKAC] # 581 635 1790 # # AciI: # [CCGC] # 11 154 155 242 519 580 # 1345 # # AcyI: # [GRCGYC] # 329 2677 # # AluI: # [AGCT] # 40 138 180 198 239 277 # 297 488 539 610 # # AlwNI: # [CAGNNNCTG] # 263 2743 # # ApoI: # [RAATTY] # 16 111 451 703 710 1015 # # AsuI*: # [GGNCC] # 166 447 453 573 1367 # # AvaII: # [GGWCC] # 900 2106 # # BaeI: # [ACNNNNGTAYC] # 33 33 263 1210 1467 # # BamHI: # [GGATCC] # 45 123 456 810 1572 # # BbvI: # [GCAGC] # 16 47 177 192 441 482 # 503 551 597 # # BbvII*: # [GAAGAC] # 25 741 983 1257 # # BccI: # [CCATC] # 37 54 150 276 519 735 # 1235 # # Bce83I*: # [CTTGAG] # 1415 1591 # # BceAI: # [ACGGC] # 227 518 1080 1181 # # BciVI: # [GTATCC] # 264 2742 # # BdaI: # [TGANNNNNNTCA] # 0 0 0 0 0 0 # 34 34 34 178 257 1058 # 1411 # # BetI*: # [WCCGGW] # 378 2628 # # BglI: # [GCCNNNNNGGC] # 320 2686 # # BglII: # [AGATCT] # 729 1067 1210 # # BinI*: # [GGATC] # 4 9 13 13 13 17 # 21 24 37 44 110 252 # 315 443 504 1187 # # BisI: # [GCNGC] # 9 47 154 177 208 408 # 441 457 494 611 # # BlsI: # [GCNGC] # 9 47 153 177 208 408 # 441 457 494 612 # # BmgT120I: # [GGNCC] # 166 446 453 573 1368 # # BsaBI: # [GATNNNNATC] # 728 2278 # # BseBI: # [CCWGG] # 113 120 254 437 823 1259 # # BseGI: # [GGATG] # 87 113 131 141 654 857 # 1023 # # BseMII: # [CTCAG] # 36 67 73 121 145 352 # 738 1474 # # BseSI: # [GKGCMC] # 1265 1741 # # BsiI*: # [CACGAG] # 387 2619 # # BsiYI*: # [CCNNNNNNNGG] # 26 209 236 492 510 553 # 980 # # BslFI: # [GGGAC] # 159 163 432 2252 # # BsmAI: # [GTCTC] # 27 56 61 206 505 606 # 655 890 # # BsmI: # [GAATGC] # 928 939 1139 # # Bsp1407I: # [TGTACA] # 383 2623 # # BspCNI: # [CTCAG] # 34 65 72 121 146 354 # 738 1476 # # BspMI: # [ACCTGC] # 440 504 2062 # # BsrI: # [ACTGG] # 36 41 181 583 615 658 # 892 # # BssKI: # [CCNGG] # 11 57 102 120 254 437 # 764 1261 # # BstEII: # [GGTNACC] # 937 2069 # # BstKTI: # [GATC] # 9 21 23 24 30 42 # 44 48 104 123 138 171 # 191 252 296 313 315 415 # 447 # # BstXI: # [CCANNNNNNTGG] # 348 651 651 1356 # # BtgZI: # [GCGATG] # 743 1064 1199 # # BtsI: # [GCAGTG] # 1267 1739 # # Cac8I: # [GCNNGC] # 7 29 64 192 201 286 # 1047 1180 # # CauII*: # [CCSGG] # 766 970 1270 # # Cfr10I: # [RCCGGY] # 326 2680 # # CfrI: # [YGGCCR] # 649 2357 # # ClaI: # [ATCGAT] # 1381 1625 # # Csp6I: # [GTAC] # 27 47 48 54 116 155 # 162 224 229 271 357 360 # 956 # # CspCI: # [CAANNNNNGTGG] # 35 848 2123 # # CviAII: # [CATG] # 9 137 143 201 213 372 # 606 1325 # # CviJI: # [RGCY] # 6 12 14 21 23 25 # 27 32 36 37 40 41 # 41 57 63 67 68 69 # 75 84 87 93 111 113 # 155 207 250 277 395 480 # # CviRI*: # [TGCA] # 40 275 364 367 877 1083 # # DdeI: # [CTNAG] # 11 13 44 59 110 158 # 159 169 235 345 375 389 # 939 # # DpnI: # [GATC] # 9 21 23 24 30 43 # 44 48 103 123 138 171 # 191 252 296 313 315 415 # 447 # # DraII: # [RGGNCCY] # 1473 1533 # # DraIII: # [CACNNNGTG] # 1401 1605 # # DrdI: # [GACNNNNNNGTC] # 237 334 2435 # # DsaI*: # [CCRYGG] # 1163 1843 # # Eam1105I: # [GACNNNNNGTC] # 1207 1799 # # EciI: # [GGCGGA] # 745 2261 # # Eco31I: # [GGTCTC] # 83 1317 1606 # # Eco57I: # [CTGAAG] # 40 895 2071 # # Eco57MI: # [CTGRAG] # 40 372 398 455 523 1218 # # EcoP15I: # [CAGCAG] # 1294 1712 # # EcoRII: # [CCWGG] # 113 120 254 437 821 1261 # # EcoRV: # [GATATC] # 495 2511 # # FalI: # [AAGNNNNNCTT] # 0 0 0 0 32 32 # 196 1216 1530 # # FatI: # [CATG] # 9 136 143 201 213 373 # 606 1325 # # FauI: # [CCCGC] # 18 1488 1500 # # FokI: # [GGATG] # 80 113 118 141 674 857 # 1023 # # GlaI: # [GCGC] # 93 147 868 1898 # # GsuI: # [CTGGAG] # 398 523 867 1218 # # HaeII: # [RGCGCY] # 95 2911 # # HaeIII: # [GGCC] # 6 14 32 41 79 201 # 446 819 1368 # # HgaI: # [GACGC] # 42 2964 # # HgiAI*: # [GWGCWC] # 551 2455 # # HgiCI*: # [GGYRCC] # 249 428 504 844 981 # # HgiJII*: # [GRGCYC] # 978 2028 # # HhaI: # [GCGC] # 94 147 868 1897 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 0 0 # 0 0 7 7 25 32 # 32 32 116 144 676 890 # 1045 # # Hin4II*: # [CCTTC] # 14 57 119 206 226 494 # 538 556 796 # # Hin6I: # [GCGC] # 92 147 868 1899 # # HindII: # [GTYRAC] # 193 580 1023 1210 # # HindIII: # [AAGCTT] # 277 1057 1672 # # HinfI: # [GANTC] # 18 21 56 63 65 66 # 69 89 122 124 180 197 # 220 235 275 288 439 479 # # HpaI: # [GTTAAC] # 1023 1983 # # HpaII: # [CCGG] # 325 379 387 946 969 # # HphI: # [GGTGA] # 39 445 949 1573 # # Hpy166II: # [GTNNAC] # 2 39 74 87 149 184 # 193 309 384 414 536 635 # # Hpy178III*: # [TCNNGA] # 17 57 64 76 88 123 # 135 146 291 388 392 587 # 642 # # Hpy188I: # [TCNGA] # 6 36 59 62 67 90 # 90 90 95 105 129 191 # 205 294 336 380 771 # # Hpy99I: # [CGWCG] # 66 192 246 265 444 1793 # # KpnI: # [GGTACC] # 1177 1829 # # Ksp632I*: # [CTCTTC] # 25 1055 1926 # # MaeI: # [CTAG] # 34 121 122 279 324 352 # 363 504 907 # # MaeII: # [ACGT] # 18 51 56 85 188 189 # 243 259 260 380 589 688 # # MaeIII: # [GTNAC] # 54 86 131 167 230 269 # 323 456 464 826 # # MboI: # [GATC] # 9 21 23 24 30 44 # 45 48 101 123 138 171 # 191 252 296 313 315 415 # 447 # # MboII: # [GAAGA] # 25 38 38 42 55 131 # 135 605 912 1025 # # McrI*: # [CGRYCG] # 594 2412 # # MfeI: # [CAATTG] # 293 1125 1588 # # MlyI: # [GAGTC] # 54 139 180 266 387 637 # 1343 # # MmeI: # [TCCRAC] # 315 688 882 1121 # # MnlI: # [CCTC] # 17 22 27 30 39 46 # 47 48 82 86 89 103 # 114 115 122 158 251 253 # 258 465 634 # # MseI: # [TTAA] # 8 9 12 49 72 92 # 94 94 103 113 114 120 # 124 159 186 194 354 401 # 708 # # MwoI: # [GCNNNNNNNGC] # 3 9 9 9 13 56 # 87 121 199 528 862 1110 # # NdeI: # [CATATG] # 895 2111 # # NlaIII: # [CATG] # 9 140 143 201 213 369 # 606 1325 # # NlaIV: # [GGNNCC] # 39 43 51 123 196 209 # 249 253 308 552 983 # # NspI: # [RCATGY] # 143 1197 1666 # # PfoI: # [TCCNGGA] # 431 437 764 1374 # # PleI: # [GAGTC] # 55 138 180 267 386 636 # 1344 # # PshAI: # [GACNNNNGTC] # 1286 1720 # # PvuI: # [CGATCG] # 594 2412 # # RsaI: # [GTAC] # 27 47 48 54 116 156 # 162 223 229 271 357 360 # 956 # # SalI: # [GTCGAC] # 582 2424 # # SauI*: # [CCTNAGG] # 540 910 1556 # # ScaI: # [AGTACT] # 772 2234 # # ScrFI: # [CCNGG] # 11 57 102 120 254 437 # 766 1259 # # SduI: # [GDGCHC] # 287 551 978 1190 # # SecI*: # [CCNNGG] # 6 98 247 335 341 554 # 603 822 # # SetI: # [ASST] # 6 8 9 11 11 12 # 12 14 15 17 17 17 # 18 19 21 21 25 27 # 27 27 29 30 36 37 # 40 40 53 56 56 66 # 68 71 85 92 97 101 # 105 106 111 118 123 163 # 182 184 184 200 239 # # SexAI: # [ACCWGGT] # 941 2065 # # SfaNI: # [GCATC] # 20 96 174 480 528 766 # 942 # # SfeI*: # [CTRYAG] # 779 2227 # # SmlI: # [CTYRAG] # 1430 1576 # # SspI: # [AATATT] # 831 1074 1101 # # StuI: # [AGGCCT] # 32 653 874 1447 # # StyI: # [CCWWGG] # 6 652 2348 # # TaiI: # [ACGT] # 18 51 56 82 188 189 # 243 259 263 380 589 688 # # TaqI: # [TCGA] # 9 70 131 145 181 339 # 351 478 502 800 # # TaqII: # [CACCCA] # 258 417 526 1805 # # TatI: # [WGTACW] # 75 383 387 407 1754 # # TauI: # [GCSGC] # 152 2854 # # TfiI: # [GAWTC] # 21 56 63 89 122 220 # 284 288 301 439 479 644 # # TseI: # [GCWGC] # 9 47 177 208 441 457 # 494 563 610 # # TsoI: # [TARCCA] # 657 818 1531 # # Tsp45I: # [GTSAC] # 54 361 456 522 1613 # # Tsp4CI*: # [ACNGT] # 116 152 278 458 479 659 # 864 # # TspDTI: # [ATGAA] # 10 52 70 79 135 162 # 173 198 246 291 400 1190 # # TspEI: # [AATT] # 14 16 17 20 22 23 # 26 30 32 47 49 61 # 62 62 71 91 98 105 # 120 136 152 170 173 199 # 239 255 268 448 # # TspGWI: # [ACGGA] # 42 1086 1878 # # TspRI: # [CASTGNN] # 27 470 790 847 872 # # VspI: # [ATTAAT] # 497 2509 # # XbaI: # [TCTAGA] # 445 642 855 1064 # # XcmI: # [CCANNNNNNNNNTGG] # 207 621 1064 1114 # # XhoII: # [RGATCY] # 9 24 45 123 191 252 # 343 380 447 463 729 # # XmnI: # [GAANNNNTTC] # 479 785 1742 # # ZraI: # [GACGTC] # 330 2676 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 3006 # Reported_sequences: 1 # Reported_hitcount: 820 #---------------------------------------