Restriction Fragments for SIN3/YOL004W



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The currently selected gene/sequence is :

SIN3/YOL004W on chromosome XV from coordinates 316938 to 321548.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.3722.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 7 + Tsp45I GTSAC 2 7 . . 3 7 + MaeIII GTNAC 2 7 . . 8 11 + SetI ASST 11 7 . . 19 22 + TspEI AATT 18 22 . . 21 26 + AsuII TTCGAA 22 24 . . 22 25 + TaqI TCGA 22 24 . . 24 29 + EcoRI GAATTC 24 28 . . 24 29 + ApoI RAATTY 24 28 . . 25 28 + TspEI AATT 24 28 . . 35 46 + CspCI CAANNNNNGTGG 23 21 58 56 45 48 + CviJI RGCY 46 46 . . 44 49 - TsoI TARCCA 34 32 . . 58 62 + HgaI GACGC 67 72 . . 67 72 + NlaIV GGNNCC 69 69 . . 70 75 + MmeI TCCRAC 95 93 . . 91 102 + PpiI GAACNNNNNCTC 83 78 115 110 91 103 + AloI GAACNNNNNNTCC 83 78 115 110 93 97 + Tsp4CI* ACNGT 95 94 . . 93 103 + BsaXI ACNNNNNCTCC 83 80 113 110 99 102 + MnlI CCTC 109 108 . . 96 100 - BslFI GGGAC 81 85 . . 102 106 + BssKI CCNGG 101 106 . . 102 106 + BseBI CCWGG 103 104 . . 102 107 + SecI* CCNNGG 102 106 . . 102 106 + ScrFI CCNGG 103 104 . . 102 106 + EcoRII CCWGG 101 106 . . 100 105 - Eco57MI CTGRAG 85 83 . . 100 105 - GsuI CTGGAG 85 83 . . 113 116 + CviJI RGCY 114 114 . . 115 119 + BssKI CCNGG 114 119 . . 115 119 + ScrFI CCNGG 116 117 . . 115 119 + CauII* CCSGG 116 117 . . 116 119 + HpaII CCGG 116 118 . . 130 135 + EcoP15I CAGCAG 160 162 . . 132 136 + BlsI GCNGC 134 133 . . 132 136 + BisI GCNGC 133 134 . . 132 136 + BbvI GCAGC 144 148 . . 132 136 + TseI GCWGC 132 135 . . 138 142 + TspGWI ACGGA 153 151 . . 152 155 + MnlI CCTC 162 161 . . 165 170 + SmlI CTYRAG 165 169 . . 165 170 + Bce83I* CTTGAG 186 184 . . 169 174 + TatI WGTACW 169 173 . . 169 174 + ScaI AGTACT 171 171 . . 170 173 + Csp6I GTAC 170 172 . . 170 173 + RsaI GTAC 171 171 . . 173 177 + DdeI CTNAG 173 176 . . 178 181 - MnlI CCTC 171 170 . . 181 185 + MboII GAAGA 193 192 . . 188 192 + MboII GAAGA 200 199 . . 188 197 + XmnI GAANNNNTTC 192 192 . . 193 197 + TfiI GAWTC 193 196 . . 193 197 + HinfI GANTC 193 196 . . 188 193 - Ksp632I* CTCTTC 183 186 . . 201 206 + BalI TGGCCA 203 203 . . 201 206 + CfrI YGGCCR 201 205 . . 202 205 + CviJI RGCY 203 203 . . 202 205 + HaeIII GGCC 203 203 . . 210 213 + CviJI RGCY 211 211 . . 223 226 + FatI CATG 222 226 . . 223 226 + NlaIII CATG 226 222 . . 223 226 + CviAII CATG 223 225 . . 226 230 + BlsI GCNGC 228 227 . . 226 230 + BisI GCNGC 227 228 . . 226 230 + TseI GCWGC 226 229 . . 226 230 - BbvI GCAGC 213 217 . . 240 243 + SetI ASST 243 239 . . 247 250 + MnlI CCTC 257 256 . . 256 265 + MslI CAYNNNNRTG 260 260 . . 272 275 + MnlI CCTC 282 281 . . 275 280 + MfeI CAATTG 275 279 . . 276 279 + TspEI AATT 275 279 . . 279 282 + CviRI* TGCA 280 280 . . 286 290 + TfiI GAWTC 286 289 . . 286 290 + HinfI GANTC 286 289 . . 302 305 + CviJI RGCY 303 303 . . 307 312 + BsiI* CACGAG 307 311 . . 319 322 + MnlI CCTC 329 328 . . 315 319 - HphI GGTGA 307 306 . . 331 335 - MboII GAAGA 323 322 . . 338 341 + MnlI CCTC 348 347 . . 339 344 + Ksp632I* CTCTTC 345 348 . . 340 344 - MboII GAAGA 332 331 . . 347 350 + MnlI CCTC 357 356 . . 367 371 + TspDTI ATGAA 382 380 . . 369 374 + BsmI GAATGC 375 373 . . 373 383 + MwoI GCNNNNNNNGC 379 376 . . 376 380 + BmgT120I GGNCC 377 378 . . 376 380 + AvaII GGWCC 376 379 . . 376 380 + AsuI* GGNCC 376 379 . . 381 384 + SetI ASST 384 380 . . 381 384 + CviJI RGCY 382 382 . . 381 384 + AluI AGCT 382 382 . . 387 390 + MnlI CCTC 397 396 . . 390 400 + BsiYI* CCNNNNNNNGG 396 393 . . 391 401 + BsiYI* CCNNNNNNNGG 397 394 . . 394 398 + Tsp4CI* ACNGT 396 395 . . 395 401 + TspRI CASTGNN 401 392 . . 400 405 + NlaIV GGNNCC 402 402 . . 402 405 + CviJI RGCY 403 403 . . 403 407 + TauI GCSGC 406 403 . . 403 407 + BlsI GCNGC 405 404 . . 403 407 + BisI GCNGC 404 405 . . 404 407 + AciI CCGC 404 406 . . 407 411 - BsrI ACTGG 407 405 . . 421 424 + TaqI TCGA 421 423 . . 421 426 + Hpy178III* TCNNGA 422 424 . . 444 454 + Hin4I GAYNNNNNVTC 435 430 467 462 450 453 + SetI ASST 453 449 . . 450 453 + CviJI RGCY 451 451 . . 450 453 + AluI AGCT 451 451 . . 458 462 - TspDTI ATGAA 448 446 . . 468 473 + NlaIV GGNNCC 470 470 . . 466 469 - MnlI CCTC 459 458 . . 480 485 + PsiI TTATAA 482 482 . . 492 497 + Hpy178III* TCNNGA 493 495 . . 489 493 - MboII GAAGA 481 480 . . 488 493 - BbvII* GAAGAC 481 485 . . 490 495 - Eco57MI CTGRAG 475 473 . . 490 495 - Eco57I CTGAAG 475 473 . . 496 500 + MboII GAAGA 508 507 . . 499 503 + MboII GAAGA 511 510 . . 509 513 + Hpy99I CGWCG 513 508 . . 510 514 + HgaI GACGC 519 524 . . 516 520 + BceAI ACGGC 532 534 . . 518 521 + CviJI RGCY 519 519 . . 518 521 + HaeIII GGCC 519 519 . . 520 523 + MnlI CCTC 530 529 . . 521 526 + SmlI CTYRAG 521 525 . . 522 527 + Hpy178III* TCNNGA 523 525 . . 521 526 - Bce83I* CTTGAG 506 504 . . 529 532 + CviRI* TGCA 530 530 . . 541 545 + FauI CCCGC 549 551 . . 542 547 + NspBII* CMGCKG 544 544 . . 542 545 + AciI CCGC 542 544 . . 550 555 + BsgI GTGCAG 571 569 . . 551 554 + CviRI* TGCA 552 552 . . 552 556 + BlsI GCNGC 554 553 . . 552 556 + BisI GCNGC 553 554 . . 552 556 + BbvI GCAGC 564 568 . . 552 556 + TseI GCWGC 552 555 . . 554 557 + CviJI RGCY 555 555 . . 555 560 + Cac8I GCNNGC 557 557 . . 557 562 - EcoP15I CAGCAG 529 531 . . 562 566 - SfaNI GCATC 552 556 . . 575 578 + MaeI CTAG 575 577 . . 578 582 + BlsI GCNGC 580 579 . . 578 582 + BisI GCNGC 579 580 . . 578 582 + BbvI GCAGC 590 594 . . 578 582 + TseI GCWGC 578 581 . . 586 595 + MslI CAYNNNNRTG 590 590 . . 586 589 + FatI CATG 585 589 . . 586 589 + NlaIII CATG 589 585 . . 586 589 + CviAII CATG 586 588 . . 588 591 + CviRI* TGCA 589 589 . . 592 596 - SfaNI GCATC 582 586 . . 621 626 + ApoI RAATTY 621 625 . . 622 625 + TspEI AATT 621 625 . . 627 632 + Hpy178III* TCNNGA 628 630 . . 630 641 + BcgI CGANNNNNNTGC 619 617 653 651 632 636 + TspDTI ATGAA 647 645 . . 634 638 + MboII GAAGA 646 645 . . 637 641 - SfaNI GCATC 627 631 . . 645 650 + SfeI* CTRYAG 645 649 . . 651 654 + SetI ASST 654 650 . . 652 655 + MnlI CCTC 662 661 . . 659 662 + TaiI ACGT 662 658 . . 659 662 + SetI ASST 662 658 . . 659 662 + MaeII ACGT 659 661 . . 666 670 + FokI GGATG 679 683 . . 666 670 + BseGI GGATG 672 670 . . 663 667 - Hin4II* CCTTC 657 656 . . 667 677 + Hin4I GAYNNNNNVTC 658 653 690 685 667 671 - SfaNI GCATC 657 661 . . 667 677 - Hin4I GAYNNNNNVTC 690 685 658 653 680 683 + SetI ASST 683 679 . . 681 684 + MnlI CCTC 691 690 . . 681 691 + EcoNI CCTNNNNNAGG 685 686 . . 681 691 + BsiYI* CCNNNNNNNGG 687 684 . . 683 686 + TaqI TCGA 683 685 . . 689 692 + SetI ASST 692 688 . . 694 699 + ApoI RAATTY 694 698 . . 695 698 + TspEI AATT 694 698 . . 701 704 + TspEI AATT 700 704 . . 710 713 + FnuDII* CGCG 711 711 . . 714 719 + Hpy178III* TCNNGA 715 717 . . 714 719 + BetI* WCCGGW 714 718 . . 714 719 + BspMII* TCCGGA 714 718 . . 711 715 - HgaI GACGC 700 705 . . 715 718 + HpaII CCGG 715 717 . . 718 723 + EcoRV GATATC 720 720 . . 727 730 + TspEI AATT 726 730 . . 745 749 + TspDTI ATGAA 760 758 . . 747 751 - Hin4II* CCTTC 741 740 . . 751 761 + Hin4I GAYNNNNNVTC 742 737 774 769 754 759 + AhaIII* TTTAAA 756 756 . . 755 758 + MseI TTAA 755 757 . . 751 761 - Hin4I GAYNNNNNVTC 774 769 742 737 761 765 + DdeI CTNAG 761 764 . . 761 765 + BspCNI CTCAG 774 772 . . 761 765 + BseMII CTCAG 775 773 . . 778 782 + BssKI CCNGG 777 782 . . 778 781 + HpaII CCGG 778 780 . . 778 782 + ScrFI CCNGG 779 780 . . 778 782 + CauII* CCSGG 779 780 . . 796 801 + BciVI GTATCC 807 806 . . 809 813 + Hpy188I TCNGA 811 810 . . 813 816 + SetI ASST 816 812 . . 812 815 - MnlI CCTC 805 804 . . 822 825 + TspEI AATT 821 825 . . 828 832 + TfiI GAWTC 828 831 . . 828 832 + HinfI GANTC 828 831 . . 855 860 + StyI CCWWGG 855 859 . . 855 860 + SecI* CCNNGG 855 859 . . 859 862 + CviJI RGCY 860 860 . . 861 866 + SfeI* CTRYAG 861 865 . . 866 870 + TfiI GAWTC 866 869 . . 866 870 + HinfI GANTC 866 869 . . 869 872 + TaqI TCGA 869 871 . . 871 876 + BsmI GAATGC 877 875 . . 878 881 + MnlI CCTC 888 887 . . 876 881 - BseRI GAGGAG 867 865 . . 885 890 + Hpy178III* TCNNGA 886 888 . . 885 890 + BetI* WCCGGW 885 889 . . 885 890 + BspMII* TCCGGA 885 889 . . 886 889 + HpaII CCGG 886 888 . . 904 908 + MaeIII GTNAC 903 908 . . 920 923 + Csp6I GTAC 920 922 . . 920 923 + RsaI GTAC 921 921 . . 928 932 + Tsp4CI* ACNGT 930 929 . . 931 936 + Hpy166II GTNNAC 933 933 . . 949 953 + BccI CCATC 957 958 . . 960 963 + SetI ASST 963 959 . . 959 962 - MnlI CCTC 952 951 . . 962 965 + Csp6I GTAC 962 964 . . 962 965 + RsaI GTAC 963 963 . . 967 971 + TspGWI ACGGA 982 980 . . 969 973 + FokI GGATG 982 986 . . 969 973 + BseGI GGATG 975 973 . . 972 975 + CviRI* TGCA 973 973 . . 970 974 - SfaNI GCATC 960 964 . . 993 998 + Hpy178III* TCNNGA 994 996 . . 1001 1006 + BtgZI GCGATG 1016 1020 . . 1003 1007 - BccI CCATC 997 998 . . 1006 1018 - AloI GAACNNNNNNTCC 1030 1025 998 993 1022 1025 + CviJI RGCY 1023 1023 . . 1025 1028 + MnlI CCTC 1035 1034 . . 1028 1042 + XcmI CCANNNNNNNNNTGG 1035 1034 . . 1035 1045 + MwoI GCNNNNNNNGC 1041 1038 . . 1041 1046 + NlaIV GGNNCC 1043 1043 . . 1041 1046 + HgiCI* GGYRCC 1041 1045 . . 1045 1049 + Hin4II* CCTTC 1055 1054 . . 1048 1051 + TaqI TCGA 1048 1050 . . 1036 1049 - AjuI GAANNNNNNNTTGG 1061 1056 1029 1024 1050 1060 - Hin4I GAYNNNNNVTC 1073 1068 1041 1036 1063 1067 + Hpy188I TCNGA 1065 1064 . . 1096 1099 + MnlI CCTC 1106 1105 . . 1104 1107 + SetI ASST 1107 1103 . . 1104 1107 + CviJI RGCY 1105 1105 . . 1104 1107 + AluI AGCT 1105 1105 . . 1111 1115 - MboII GAAGA 1103 1102 . . 1118 1123 + Hpy166II GTNNAC 1120 1120 . . 1122 1125 + SetI ASST 1125 1121 . . 1123 1127 + Hin4II* CCTTC 1133 1132 . . 1128 1131 + TspEI AATT 1127 1131 . . 1137 1140 + SetI ASST 1140 1136 . . 1146 1151 + Cac8I GCNNGC 1148 1148 . . 1149 1152 + CviRI* TGCA 1150 1150 . . 1148 1153 - BsgI GTGCAG 1133 1131 . . 1153 1158 - BtgZI GCGATG 1138 1142 . . 1170 1180 + FalI AAGNNNNNCTT 1161 1156 1193 1188 1170 1180 - FalI AAGNNNNNCTT 1193 1188 1161 1156 1186 1189 + MnlI CCTC 1196 1195 . . 1187 1192 + SmlI CTYRAG 1187 1191 . . 1188 1193 + Hpy178III* TCNNGA 1189 1191 . . 1192 1196 + MboII GAAGA 1204 1203 . . 1187 1192 - Bce83I* CTTGAG 1172 1170 . . 1195 1199 - SfaNI GCATC 1185 1189 . . 1199 1203 + DdeI CTNAG 1199 1202 . . 1207 1212 + AclI AACGTT 1208 1210 . . 1208 1211 + TaiI ACGT 1211 1207 . . 1208 1211 + SetI ASST 1211 1207 . . 1208 1211 + MaeII ACGT 1208 1210 . . 1217 1220 + TaqI TCGA 1217 1219 . . 1219 1224 + ApoI RAATTY 1219 1223 . . 1220 1223 + TspEI AATT 1219 1223 . . 1230 1233 + CviJI RGCY 1231 1231 . . 1237 1240 + SetI ASST 1240 1236 . . 1237 1240 + CviJI RGCY 1238 1238 . . 1237 1240 + AluI AGCT 1238 1238 . . 1241 1244 + TaiI ACGT 1244 1240 . . 1241 1244 + SetI ASST 1244 1240 . . 1241 1244 + MaeII ACGT 1241 1243 . . 1244 1247 + MseI TTAA 1244 1246 . . 1251 1254 + TspEI AATT 1250 1254 . . 1253 1256 + MseI TTAA 1253 1255 . . 1259 1262 + MaeI CTAG 1259 1261 . . 1275 1278 + SetI ASST 1278 1274 . . 1296 1301 + Hpy178III* TCNNGA 1297 1299 . . 1331 1334 + CviJI RGCY 1332 1332 . . 1345 1350 + Hpy166II GTNNAC 1347 1347 . . 1345 1350 + AccI GTMKAC 1346 1348 . . 1357 1361 + Tsp45I GTSAC 1356 1361 . . 1357 1361 + MaeIII GTNAC 1356 1361 . . 1359 1363 + HgaI GACGC 1368 1373 . . 1362 1366 + SfaNI GCATC 1371 1375 . . 1380 1385 + Hpy178III* TCNNGA 1381 1383 . . 1390 1393 + MaeI CTAG 1390 1392 . . 1393 1397 + MboII GAAGA 1405 1404 . . 1400 1405 + Hpy178III* TCNNGA 1401 1403 . . 1402 1412 + FalI AAGNNNNNCTT 1393 1388 1425 1420 1405 1410 + ApoI RAATTY 1405 1409 . . 1406 1409 + TspEI AATT 1405 1409 . . 1402 1412 - FalI AAGNNNNNCTT 1425 1420 1393 1388 1414 1417 + HpaII CCGG 1414 1416 . . 1417 1421 + HinfI GANTC 1417 1420 . . 1419 1424 + Ksp632I* CTCTTC 1425 1428 . . 1417 1421 - PleI GAGTC 1411 1412 . . 1417 1421 - MlyI GAGTC 1411 1411 . . 1420 1424 - MboII GAAGA 1412 1411 . . 1424 1432 + AlwNI CAGNNNCTG 1429 1426 . . 1425 1428 + SetI ASST 1428 1424 . . 1425 1428 + CviJI RGCY 1426 1426 . . 1425 1428 + AluI AGCT 1426 1426 . . 1421 1426 - Eco57MI CTGRAG 1406 1404 . . 1421 1426 - Eco57I CTGAAG 1406 1404 . . 1438 1443 + EcoP15I CAGCAG 1468 1470 . . 1442 1445 + SetI ASST 1445 1441 . . 1445 1448 + CviRI* TGCA 1446 1446 . . 1440 1445 - BspMI ACCTGC 1431 1435 . . 1440 1446 - AarI CACCTGC 1431 1435 . . 1449 1454 + NspI RCATGY 1453 1449 . . 1450 1453 + FatI CATG 1449 1453 . . 1450 1453 + NlaIII CATG 1453 1449 . . 1450 1453 + CviAII CATG 1450 1452 . . 1453 1459 + EspI* GCTNAGC 1454 1457 . . 1454 1458 + DdeI CTNAG 1454 1457 . . 1454 1458 + BspCNI CTCAG 1467 1465 . . 1454 1458 + BseMII CTCAG 1468 1466 . . 1461 1466 + NspI RCATGY 1465 1461 . . 1462 1465 + FatI CATG 1461 1465 . . 1462 1465 + NlaIII CATG 1465 1461 . . 1462 1465 + CviAII CATG 1462 1464 . . 1477 1480 + FatI CATG 1476 1480 . . 1477 1480 + NlaIII CATG 1480 1476 . . 1477 1480 + CviAII CATG 1477 1479 . . 1482 1485 + CviJI RGCY 1483 1483 . . 1482 1485 + HaeIII GGCC 1483 1483 . . 1482 1486 + BmgT120I GGNCC 1483 1484 . . 1482 1486 + AsuI* GGNCC 1482 1485 . . 1480 1483 - MnlI CCTC 1473 1472 . . 1489 1494 + EcoT22I ATGCAT 1493 1489 . . 1490 1493 + CviRI* TGCA 1491 1491 . . 1491 1496 + NspI RCATGY 1495 1491 . . 1491 1496 + Cac8I GCNNGC 1493 1493 . . 1491 1496 + SphI GCATGC 1495 1491 . . 1492 1495 + FatI CATG 1491 1495 . . 1492 1495 + NlaIII CATG 1495 1491 . . 1492 1495 + CviAII CATG 1492 1494 . . 1494 1497 + CviRI* TGCA 1495 1495 . . 1506 1509 + SetI ASST 1509 1505 . . 1506 1509 + CviJI RGCY 1507 1507 . . 1506 1509 + AluI AGCT 1507 1507 . . 1508 1512 + DdeI CTNAG 1508 1511 . . 1508 1512 + BspCNI CTCAG 1521 1519 . . 1508 1512 + BseMII CTCAG 1522 1520 . . 1512 1515 + CviJI RGCY 1513 1513 . . 1507 1513 - Bpu10I CCTNAGC 1508 1511 . . 1514 1518 + DdeI CTNAG 1514 1517 . . 1514 1518 + BspCNI CTCAG 1527 1525 . . 1514 1518 + BseMII CTCAG 1528 1526 . . 1513 1519 - Bpu10I CCTNAGC 1514 1517 . . 1529 1532 + SetI ASST 1532 1528 . . 1545 1555 + MwoI GCNNNNNNNGC 1551 1548 . . 1545 1549 + BlsI GCNGC 1547 1546 . . 1545 1549 + BisI GCNGC 1546 1547 . . 1545 1549 + BbvI GCAGC 1557 1561 . . 1545 1549 + TseI GCWGC 1545 1548 . . 1556 1559 + TspEI AATT 1555 1559 . . 1563 1568 + BciVI GTATCC 1574 1573 . . 1567 1577 + BsiYI* CCNNNNNNNGG 1573 1570 . . 1569 1572 + SetI ASST 1572 1568 . . 1569 1572 + CviJI RGCY 1570 1570 . . 1569 1572 + AluI AGCT 1570 1570 . . 1576 1579 + CviJI RGCY 1577 1577 . . 1577 1587 + MwoI GCNNNNNNNGC 1583 1580 . . 1583 1587 + BceAI ACGGC 1599 1601 . . 1584 1589 + CfrI YGGCCR 1584 1588 . . 1585 1588 + CviJI RGCY 1586 1586 . . 1585 1588 + HaeIII GGCC 1586 1586 . . 1587 1591 + BccI CCATC 1595 1596 . . 1591 1595 + Hin4II* CCTTC 1601 1600 . . 1588 1592 - FokI GGATG 1574 1578 . . 1588 1592 - BseGI GGATG 1587 1585 . . 1599 1604 + SecI* CCNNGG 1599 1603 . . 1602 1605 + SetI ASST 1605 1601 . . 1601 1604 - MnlI CCTC 1594 1593 . . 1614 1624 + MwoI GCNNNNNNNGC 1620 1617 . . 1614 1624 + BstAPI GCANNNNNTGC 1620 1617 . . 1624 1627 + MnlI CCTC 1634 1633 . . 1624 1634 + EcoNI CCTNNNNNAGG 1628 1629 . . 1624 1634 + BsiYI* CCNNNNNNNGG 1630 1627 . . 1628 1633 + SfeI* CTRYAG 1628 1632 . . 1635 1640 + HindIII AAGCTT 1635 1639 . . 1636 1639 + SetI ASST 1639 1635 . . 1636 1639 + CviJI RGCY 1637 1637 . . 1636 1639 + AluI AGCT 1637 1637 . . 1645 1648 + MnlI CCTC 1655 1654 . . 1647 1652 + MmeI TCCRAC 1672 1670 . . 1648 1658 + BsiYI* CCNNNNNNNGG 1654 1651 . . 1655 1659 + Tsp4CI* ACNGT 1657 1656 . . 1660 1663 + TaqI TCGA 1660 1662 . . 1661 1666 + McrI* CGRYCG 1664 1662 . . 1648 1661 - AjuI GAANNNNNNNTTGG 1673 1668 1641 1636 1663 1667 + Tsp4CI* ACNGT 1665 1664 . . 1666 1669 + Csp6I GTAC 1666 1668 . . 1666 1669 + RsaI GTAC 1667 1667 . . 1669 1672 + FatI CATG 1668 1672 . . 1669 1672 + NlaIII CATG 1672 1668 . . 1669 1672 + CviAII CATG 1669 1671 . . 1670 1674 + TspDTI ATGAA 1685 1683 . . 1671 1682 + BdaI TGANNNNNNTCA 1660 1658 1694 1692 1674 1677 + CviJI RGCY 1675 1675 . . 1680 1685 + Hpy178III* TCNNGA 1681 1683 . . 1671 1682 - BdaI TGANNNNNNTCA 1694 1692 1660 1658 1683 1687 + BinI* GGATC 1691 1692 . . 1684 1687 + DpnI GATC 1685 1685 . . 1684 1687 + BstKTI GATC 1686 1684 . . 1684 1687 + MboI GATC 1683 1687 . . 1694 1699 + NspI RCATGY 1698 1694 . . 1695 1698 + FatI CATG 1694 1698 . . 1695 1698 + NlaIII CATG 1698 1694 . . 1695 1698 + CviAII CATG 1695 1697 . . 1697 1700 + CviRI* TGCA 1698 1698 . . 1713 1716 + FatI CATG 1712 1716 . . 1713 1716 + NlaIII CATG 1716 1712 . . 1713 1716 + CviAII CATG 1713 1715 . . 1711 1715 - TspDTI ATGAA 1701 1699 . . 1723 1727 + BccI CCATC 1731 1732 . . 1725 1730 + ClaI ATCGAT 1726 1728 . . 1726 1729 + TaqI TCGA 1726 1728 . . 1727 1732 + McrI* CGRYCG 1730 1728 . . 1727 1732 + PvuI CGATCG 1730 1728 . . 1728 1731 + DpnI GATC 1729 1729 . . 1728 1731 + BstKTI GATC 1730 1728 . . 1728 1731 + MboI GATC 1727 1731 . . 1741 1745 + BmgT120I GGNCC 1742 1743 . . 1741 1745 + AsuI* GGNCC 1741 1744 . . 1742 1745 + CviJI RGCY 1743 1743 . . 1742 1745 + HaeIII GGCC 1743 1743 . . 1753 1756 + Csp6I GTAC 1753 1755 . . 1753 1756 + RsaI GTAC 1754 1754 . . 1772 1776 + TspDTI ATGAA 1787 1785 . . 1782 1785 + SetI ASST 1785 1781 . . 1783 1786 + MnlI CCTC 1793 1792 . . 1789 1793 + Hpy188I TCNGA 1791 1790 . . 1791 1796 + XhoII RGATCY 1791 1795 . . 1791 1796 + BglII AGATCT 1791 1795 . . 1792 1795 + DpnI GATC 1793 1793 . . 1792 1795 + BstKTI GATC 1794 1792 . . 1792 1795 + MboI GATC 1791 1795 . . 1795 1799 + DdeI CTNAG 1795 1798 . . 1801 1804 + TaiI ACGT 1804 1800 . . 1801 1804 + SetI ASST 1804 1800 . . 1801 1804 + MaeII ACGT 1801 1803 . . 1802 1807 + Esp3I CGTCTC 1808 1812 . . 1803 1807 + BsmAI GTCTC 1808 1812 . . 1809 1815 + TspRI CASTGNN 1815 1806 . . 1815 1819 + Tsp4CI* ACNGT 1817 1816 . . 1818 1821 + Csp6I GTAC 1818 1820 . . 1818 1821 + RsaI GTAC 1819 1819 . . 1825 1829 + Hin4II* CCTTC 1835 1834 . . 1829 1832 + MaeI CTAG 1829 1831 . . 1832 1837 + Hpy166II GTNNAC 1834 1834 . . 1832 1837 + BssNAI GTATAC 1834 1834 . . 1832 1837 + AccI GTMKAC 1833 1835 . . 1843 1848 + EcoP15I CAGCAG 1873 1875 . . 1845 1849 + BlsI GCNGC 1847 1846 . . 1845 1849 + BisI GCNGC 1846 1847 . . 1845 1849 + BbvI GCAGC 1857 1861 . . 1845 1849 + TseI GCWGC 1845 1848 . . 1846 1851 + EcoP15I CAGCAG 1876 1878 . . 1848 1858 + MwoI GCNNNNNNNGC 1854 1851 . . 1848 1852 + BlsI GCNGC 1850 1849 . . 1848 1852 + BisI GCNGC 1849 1850 . . 1848 1852 + BbvI GCAGC 1860 1864 . . 1848 1852 + TseI GCWGC 1848 1851 . . 1854 1858 + FauI CCCGC 1862 1864 . . 1855 1858 + AciI CCGC 1855 1857 . . 1871 1876 + SfeI* CTRYAG 1871 1875 . . 1891 1895 + HphI GGTGA 1903 1902 . . 1894 1899 + EcoRV GATATC 1896 1896 . . 1908 1913 + BetI* WCCGGW 1908 1912 . . 1909 1912 + HpaII CCGG 1909 1911 . . 1914 1917 + TspEI AATT 1913 1917 . . 1918 1927 + BsaBI GATNNNNATC 1922 1922 . . 1923 1928 + XhoII RGATCY 1923 1927 . . 1924 1927 + DpnI GATC 1925 1925 . . 1924 1927 + BstKTI GATC 1926 1924 . . 1924 1927 + MboI GATC 1923 1927 . . 1928 1931 + MaeI CTAG 1928 1930 . . 1924 1928 - BinI* GGATC 1918 1919 . . 1944 1949 + NlaIV GGNNCC 1946 1946 . . 1944 1948 + BmgT120I GGNCC 1945 1946 . . 1944 1948 + AvaII GGWCC 1944 1947 . . 1944 1948 + AsuI* GGNCC 1944 1947 . . 1945 1949 - BslFI GGGAC 1930 1934 . . 1956 1962 + TspRI CASTGNN 1962 1953 . . 1958 1962 + DdeI CTNAG 1958 1961 . . 1960 1965 + SduI GDGCHC 1964 1960 . . 1960 1965 + HgiJII* GRGCYC 1964 1960 . . 1961 1964 + CviJI RGCY 1962 1962 . . 1958 1962 - BspCNI CTCAG 1950 1948 . . 1958 1962 - BseMII CTCAG 1949 1947 . . 1964 1968 + BccI CCATC 1972 1973 . . 1967 1970 + TaqI TCGA 1967 1969 . . 1985 1988 + MseI TTAA 1985 1987 . . 1990 1993 - MnlI CCTC 1983 1982 . . 1996 2000 + Tsp45I GTSAC 1995 2000 . . 1996 2000 + MaeIII GTNAC 1995 2000 . . 2005 2010 + AsuII TTCGAA 2006 2008 . . 2006 2009 + TaqI TCGA 2006 2008 . . 2003 2007 - MboII GAAGA 1995 1994 . . 2013 2016 + CviJI RGCY 2014 2014 . . 2013 2016 + HaeIII GGCC 2014 2014 . . 2020 2023 + SetI ASST 2023 2019 . . 2019 2022 - MnlI CCTC 2012 2011 . . 2046 2052 + TspRI CASTGNN 2052 2043 . . 2048 2052 + DdeI CTNAG 2048 2051 . . 2048 2052 - BspCNI CTCAG 2040 2038 . . 2048 2052 - BseMII CTCAG 2039 2037 . . 2060 2065 + ApoI RAATTY 2060 2064 . . 2061 2064 + TspEI AATT 2060 2064 . . 2064 2069 + AhaIII* TTTAAA 2066 2066 . . 2065 2068 + MseI TTAA 2065 2067 . . 2067 2072 + ApoI RAATTY 2067 2071 . . 2068 2071 + TspEI AATT 2067 2071 . . 2072 2077 + TatI WGTACW 2072 2076 . . 2073 2076 + Csp6I GTAC 2073 2075 . . 2073 2076 + RsaI GTAC 2074 2074 . . 2078 2083 + SmlI CTYRAG 2078 2082 . . 2079 2084 + Hpy178III* TCNNGA 2080 2082 . . 2081 2091 + FalI AAGNNNNNCTT 2072 2067 2104 2099 2078 2083 - Bce83I* CTTGAG 2063 2061 . . 2081 2091 - FalI AAGNNNNNCTT 2104 2099 2072 2067 2092 2095 + DpnI GATC 2093 2093 . . 2092 2095 + BstKTI GATC 2094 2092 . . 2092 2095 + MboI GATC 2091 2095 . . 2094 2099 + Hpy178III* TCNNGA 2095 2097 . . 2114 2117 + SetI ASST 2117 2113 . . 2131 2136 + NlaIV GGNNCC 2133 2133 . . 2151 2156 + BsaAI YACGTR 2153 2153 . . 2152 2155 + TaiI ACGT 2155 2151 . . 2152 2155 + SetI ASST 2155 2151 . . 2152 2155 + MaeII ACGT 2152 2154 . . 2173 2176 + CviJI RGCY 2174 2174 . . 2172 2177 - TsoI TARCCA 2162 2160 . . 2198 2201 + TaqI TCGA 2198 2200 . . 2198 2203 + Hpy178III* TCNNGA 2199 2201 . . 2203 2208 + SspI AATATT 2205 2205 . . 2211 2216 + Hpy178III* TCNNGA 2212 2214 . . 2211 2216 + BspHI TCATGA 2211 2215 . . 2212 2215 + FatI CATG 2211 2215 . . 2212 2215 + NlaIII CATG 2215 2211 . . 2212 2215 + CviAII CATG 2212 2214 . . 2213 2217 + TspDTI ATGAA 2228 2226 . . 2210 2214 - TspDTI ATGAA 2200 2198 . . 2245 2248 - MnlI CCTC 2238 2237 . . 2254 2257 + CviJI RGCY 2255 2255 . . 2254 2257 + HaeIII GGCC 2255 2255 . . 2254 2258 + BmgT120I GGNCC 2255 2256 . . 2254 2258 + AsuI* GGNCC 2254 2257 . . 2261 2265 + MaeIII GTNAC 2260 2265 . . 2270 2273 + CviJI RGCY 2271 2271 . . 2268 2271 - MnlI CCTC 2261 2260 . . 2280 2290 + FalI AAGNNNNNCTT 2271 2266 2303 2298 2282 2286 + Tsp45I GTSAC 2281 2286 . . 2282 2286 + MaeIII GTNAC 2281 2286 . . 2286 2295 + MslI CAYNNNNRTG 2290 2290 . . 2280 2290 - FalI AAGNNNNNCTT 2303 2298 2271 2266 2292 2295 + FatI CATG 2291 2295 . . 2292 2295 + NlaIII CATG 2295 2291 . . 2292 2295 + CviAII CATG 2292 2294 . . 2290 2294 - TspDTI ATGAA 2280 2278 . . 2296 2306 + BsiYI* CCNNNNNNNGG 2302 2299 . . 2297 2300 + FatI CATG 2296 2300 . . 2297 2300 + NlaIII CATG 2300 2296 . . 2297 2300 + CviAII CATG 2297 2299 . . 2301 2305 + DdeI CTNAG 2301 2304 . . 2301 2305 + BspCNI CTCAG 2314 2312 . . 2301 2305 + BseMII CTCAG 2315 2313 . . 2304 2307 + SetI ASST 2307 2303 . . 2300 2306 - Bpu10I CCTNAGC 2301 2304 . . 2310 2314 + FokI GGATG 2323 2327 . . 2310 2314 + BseGI GGATG 2316 2314 . . 2310 2321 - TstI CACNNNNNNTCC 2334 2329 2302 2297 2330 2335 + Hpy178III* TCNNGA 2331 2333 . . 2339 2343 + TspDTI ATGAA 2354 2352 . . 2347 2352 - MmeI TCCRAC 2328 2326 . . 2353 2357 - FokI GGATG 2339 2343 . . 2353 2357 - BseGI GGATG 2352 2350 . . 2364 2367 + CviJI RGCY 2365 2365 . . 2368 2372 + Hpy188I TCNGA 2370 2369 . . 2371 2375 + MboII GAAGA 2383 2382 . . 2374 2378 + TfiI GAWTC 2374 2377 . . 2374 2378 + HinfI GANTC 2374 2377 . . 2377 2382 + Hpy178III* TCNNGA 2378 2380 . . 2403 2413 + BsiYI* CCNNNNNNNGG 2409 2406 . . 2403 2407 - BsrI ACTGG 2403 2401 . . 2410 2413 - MnlI CCTC 2403 2402 . . 2423 2428 + Hpy178III* TCNNGA 2424 2426 . . 2427 2430 + DpnI GATC 2428 2428 . . 2427 2430 + BstKTI GATC 2429 2427 . . 2427 2430 + MboI GATC 2426 2430 . . 2429 2432 + TaqI TCGA 2429 2431 . . 2431 2435 + MboII GAAGA 2443 2442 . . 2431 2436 - Ksp632I* CTCTTC 2426 2429 . . 2434 2437 - MnlI CCTC 2427 2426 . . 2443 2452 + MslI CAYNNNNRTG 2447 2447 . . 2443 2446 + FatI CATG 2442 2446 . . 2443 2446 + NlaIII CATG 2446 2442 . . 2443 2446 + CviAII CATG 2443 2445 . . 2464 2468 + TfiI GAWTC 2464 2467 . . 2464 2468 + HinfI GANTC 2464 2467 . . 2469 2474 + ApoI RAATTY 2469 2473 . . 2470 2473 + TspEI AATT 2469 2473 . . 2473 2476 + MseI TTAA 2473 2475 . . 2498 2503 + MfeI CAATTG 2498 2502 . . 2499 2502 + TspEI AATT 2498 2502 . . 2511 2514 + DpnI GATC 2512 2512 . . 2511 2514 + BstKTI GATC 2513 2511 . . 2511 2514 + MboI GATC 2510 2514 . . 2513 2516 + TaqI TCGA 2513 2515 . . 2513 2518 + Hpy178III* TCNNGA 2514 2516 . . 2520 2523 + FatI CATG 2519 2523 . . 2520 2523 + NlaIII CATG 2523 2519 . . 2520 2523 + CviAII CATG 2520 2522 . . 2525 2529 + DdeI CTNAG 2525 2528 . . 2525 2529 - BspCNI CTCAG 2517 2515 . . 2525 2529 - BseMII CTCAG 2516 2514 . . 2531 2535 + TspDTI ATGAA 2546 2544 . . 2538 2541 + CviJI RGCY 2539 2539 . . 2542 2545 + TspEI AATT 2541 2545 . . 2545 2550 + AhaIII* TTTAAA 2547 2547 . . 2546 2549 + MseI TTAA 2546 2548 . . 2554 2557 + MnlI CCTC 2564 2563 . . 2557 2561 + BssKI CCNGG 2556 2561 . . 2557 2561 + BseBI CCWGG 2558 2559 . . 2557 2561 + ScrFI CCNGG 2558 2559 . . 2557 2561 + EcoRII CCWGG 2556 2561 . . 2557 2567 + BsiYI* CCNNNNNNNGG 2563 2560 . . 2557 2567 + PflMI CCANNNNNTGG 2563 2560 . . 2559 2562 + SetI ASST 2562 2558 . . 2555 2560 - Eco57MI CTGRAG 2540 2538 . . 2555 2560 - GsuI CTGGAG 2540 2538 . . 2565 2570 + BalI TGGCCA 2567 2567 . . 2565 2570 + CfrI YGGCCR 2565 2569 . . 2566 2569 + CviJI RGCY 2567 2567 . . 2566 2569 + HaeIII GGCC 2567 2567 . . 2586 2591 + PsiI TTATAA 2588 2588 . . 2630 2633 + TaqI TCGA 2630 2632 . . 2630 2635 + Hpy178III* TCNNGA 2631 2633 . . 2619 2631 - AloI GAACNNNNNNTCC 2643 2638 2611 2606 2641 2645 - SfaNI GCATC 2631 2635 . . 2648 2651 + CviRI* TGCA 2649 2649 . . 2650 2653 + FatI CATG 2649 2653 . . 2650 2653 + NlaIII CATG 2653 2649 . . 2650 2653 + CviAII CATG 2650 2652 . . 2653 2658 + SduI GDGCHC 2657 2653 . . 2653 2658 + HgiAI* GWGCWC 2657 2653 . . 2660 2665 + PstI CTGCAG 2664 2660 . . 2660 2665 + SfeI* CTRYAG 2660 2664 . . 2661 2664 + CviRI* TGCA 2662 2662 . . 2662 2667 + BtsI GCAGTG 2669 2667 . . 2662 2672 + MwoI GCNNNNNNNGC 2668 2665 . . 2663 2669 + TspRI CASTGNN 2669 2660 . . 2665 2669 + Tsp45I GTSAC 2664 2669 . . 2665 2669 + MaeIII GTNAC 2664 2669 . . 2670 2673 + CviJI RGCY 2671 2671 . . 2674 2678 - BsrI ACTGG 2674 2672 . . 2673 2678 - BfiI ACTGGG 2668 2667 . . 2682 2686 + Hpy188I TCNGA 2684 2683 . . 2689 2692 + SetI ASST 2692 2688 . . 2692 2695 + MseI TTAA 2692 2694 . . 2707 2711 + MboII GAAGA 2719 2718 . . 2715 2725 + BplI GAGNNNNNCTC 2706 2701 2738 2733 2717 2721 + MboII GAAGA 2729 2728 . . 2720 2725 + SacI GAGCTC 2724 2720 . . 2720 2725 + Ecl136II GAGCTC 2722 2722 . . 2720 2725 + SduI GDGCHC 2724 2720 . . 2720 2725 + HgiJII* GRGCYC 2724 2720 . . 2720 2725 + HgiAI* GWGCWC 2724 2720 . . 2721 2724 + SetI ASST 2724 2720 . . 2721 2724 + CviJI RGCY 2722 2722 . . 2721 2724 + AluI AGCT 2722 2722 . . 2717 2722 - Ksp632I* CTCTTC 2712 2715 . . 2717 2723 - SapI GCTCTTC 2712 2715 . . 2715 2725 - BplI GAGNNNNNCTC 2738 2733 2706 2701 2727 2730 + TaiI ACGT 2730 2726 . . 2727 2730 + SetI ASST 2730 2726 . . 2727 2730 + MaeII ACGT 2727 2729 . . 2748 2751 - MnlI CCTC 2741 2740 . . 2765 2768 + SetI ASST 2768 2764 . . 2763 2767 - Hin4II* CCTTC 2757 2756 . . 2785 2788 + DpnI GATC 2786 2786 . . 2785 2788 + BstKTI GATC 2787 2785 . . 2785 2788 + MboI GATC 2784 2788 . . 2794 2797 + CviJI RGCY 2795 2795 . . 2797 2800 + MseI TTAA 2797 2799 . . 2803 2808 + AhaIII* TTTAAA 2805 2805 . . 2804 2807 + MseI TTAA 2804 2806 . . 2811 2814 + CviJI RGCY 2812 2812 . . 2822 2825 + TspEI AATT 2821 2825 . . 2827 2830 + MseI TTAA 2827 2829 . . 2844 2849 + EcoRV GATATC 2846 2846 . . 2848 2852 + Hpy188I TCNGA 2850 2849 . . 2858 2862 + BlsI GCNGC 2860 2859 . . 2858 2862 + BisI GCNGC 2859 2860 . . 2858 2862 + BbvI GCAGC 2870 2874 . . 2858 2862 + TseI GCWGC 2858 2861 . . 2861 2865 + SfaNI GCATC 2870 2874 . . 2871 2876 + BclI TGATCA 2871 2875 . . 2872 2875 + DpnI GATC 2873 2873 . . 2872 2875 + BstKTI GATC 2874 2872 . . 2872 2875 + MboI GATC 2871 2875 . . 2888 2893 + ApoI RAATTY 2888 2892 . . 2889 2892 + TspEI AATT 2888 2892 . . 2893 2899 + TspRI CASTGNN 2899 2890 . . 2894 2898 + BsrI ACTGG 2899 2897 . . 2898 2903 + Hpy166II GTNNAC 2900 2900 . . 2898 2903 + HpaI GTTAAC 2900 2900 . . 2898 2903 + HindII GTYRAC 2900 2900 . . 2899 2902 + MseI TTAA 2899 2901 . . 2896 2901 - TsoI TARCCA 2886 2884 . . 2917 2920 + CviJI RGCY 2918 2918 . . 2919 2923 - BsrI ACTGG 2919 2917 . . 2959 2964 + EcoRV GATATC 2961 2961 . . 2973 2976 + CviJI RGCY 2974 2974 . . 2984 2989 + PsiI TTATAA 2986 2986 . . 3000 3003 + CviJI RGCY 3001 3001 . . 3012 3017 + Hpy178III* TCNNGA 3013 3015 . . 3049 3053 - TspDTI ATGAA 3039 3037 . . 3076 3081 + AsuII TTCGAA 3077 3079 . . 3077 3080 + TaqI TCGA 3077 3079 . . 3086 3089 + TaqI TCGA 3086 3088 . . 3088 3092 + MboII GAAGA 3100 3099 . . 3091 3095 + TfiI GAWTC 3091 3094 . . 3091 3095 + HinfI GANTC 3091 3094 . . 3098 3103 + TatI WGTACW 3098 3102 . . 3099 3102 + Csp6I GTAC 3099 3101 . . 3099 3102 + RsaI GTAC 3100 3100 . . 3116 3119 + TaiI ACGT 3119 3115 . . 3116 3119 + SetI ASST 3119 3115 . . 3116 3119 + MaeII ACGT 3116 3118 . . 3116 3121 + AflIII ACRYGT 3116 3120 . . 3121 3125 + Hpy188I TCNGA 3123 3122 . . 3124 3128 + TfiI GAWTC 3124 3127 . . 3124 3128 + HinfI GANTC 3124 3127 . . 3128 3131 + MaeI CTAG 3128 3130 . . 3133 3138 + XhoII RGATCY 3133 3137 . . 3133 3137 + BinI* GGATC 3141 3142 . . 3134 3137 + DpnI GATC 3135 3135 . . 3134 3137 + BstKTI GATC 3136 3134 . . 3134 3137 + MboI GATC 3133 3137 . . 3131 3134 - AciI CCGC 3131 3133 . . 3136 3140 + Hpy188I TCNGA 3138 3137 . . 3142 3146 - BccI CCATC 3136 3137 . . 3156 3159 + CviRI* TGCA 3157 3157 . . 3157 3161 + SfaNI GCATC 3166 3170 . . 3165 3169 + MboII GAAGA 3177 3176 . . 3169 3175 + DraII RGGNCCY 3170 3173 . . 3170 3173 + CviJI RGCY 3171 3171 . . 3165 3170 - Ksp632I* CTCTTC 3160 3163 . . 3170 3173 + HaeIII GGCC 3171 3171 . . 3170 3174 + BmgT120I GGNCC 3171 3172 . . 3170 3174 + AsuI* GGNCC 3170 3173 . . 3168 3171 - MnlI CCTC 3161 3160 . . 3168 3178 - Hin4I GAYNNNNNVTC 3191 3186 3159 3154 3191 3196 + Hpy166II GTNNAC 3193 3193 . . 3218 3223 + EcoRV GATATC 3220 3220 . . 3213 3223 - Hin4I GAYNNNNNVTC 3236 3231 3204 3199 3227 3230 + SetI ASST 3230 3226 . . 3227 3230 + CviJI RGCY 3228 3228 . . 3227 3230 + AluI AGCT 3228 3228 . . 3245 3249 + TspDTI ATGAA 3260 3258 . . 3247 3251 + MboII GAAGA 3259 3258 . . 3250 3254 - BccI CCATC 3244 3245 . . 3263 3268 + GsaI CCCAGC 3267 3263 . . 3263 3268 + BseYI CCCAGC 3263 3267 . . 3266 3269 + SetI ASST 3269 3265 . . 3266 3269 + CviJI RGCY 3267 3267 . . 3266 3269 + AluI AGCT 3267 3267 . . 3271 3275 + Hpy188I TCNGA 3273 3272 . . 3283 3287 + MboII GAAGA 3295 3294 . . 3286 3290 + MboII GAAGA 3298 3297 . . 3291 3294 + SetI ASST 3294 3290 . . 3304 3309 + BseRI GAGGAG 3319 3317 . . 3304 3307 - MnlI CCTC 3297 3296 . . 3307 3310 - MnlI CCTC 3300 3299 . . 3310 3314 + MboII GAAGA 3322 3321 . . 3313 3318 + SacI GAGCTC 3317 3313 . . 3313 3318 + Ecl136II GAGCTC 3315 3315 . . 3313 3318 + SduI GDGCHC 3317 3313 . . 3313 3318 + HgiJII* GRGCYC 3317 3313 . . 3313 3318 + HgiAI* GWGCWC 3317 3313 . . 3314 3317 + SetI ASST 3317 3313 . . 3314 3317 + CviJI RGCY 3315 3315 . . 3314 3317 + AluI AGCT 3315 3315 . . 3310 3315 - Ksp632I* CTCTTC 3305 3308 . . 3310 3316 - SapI GCTCTTC 3305 3308 . . 3319 3324 + ClaI ATCGAT 3320 3322 . . 3320 3323 + TaqI TCGA 3320 3322 . . 3323 3327 + TspDTI ATGAA 3338 3336 . . 3325 3329 + MboII GAAGA 3337 3336 . . 3330 3333 + SetI ASST 3333 3329 . . 3330 3333 + CviJI RGCY 3331 3331 . . 3330 3333 + AluI AGCT 3331 3331 . . 3340 3345 + DsaI* CCRYGG 3340 3344 . . 3340 3345 + SecI* CCNNGG 3340 3344 . . 3339 3343 - TspGWI ACGGA 3329 3327 . . 3344 3347 + CviJI RGCY 3345 3345 . . 3343 3348 - TsoI TARCCA 3333 3331 . . 3349 3353 + BsrI ACTGG 3354 3352 . . 3349 3354 + BfiI ACTGGG 3359 3358 . . 3354 3359 + ApoI RAATTY 3354 3358 . . 3355 3358 + TspEI AATT 3354 3358 . . 3364 3367 - MnlI CCTC 3357 3356 . . 3377 3381 + DdeI CTNAG 3377 3380 . . 3377 3381 + BspCNI CTCAG 3390 3388 . . 3377 3381 + BseMII CTCAG 3391 3389 . . 3407 3410 + MseI TTAA 3407 3409 . . 3427 3432 + SspI AATATT 3429 3429 . . 3443 3447 - TspGWI ACGGA 3433 3431 . . 3468 3472 + TauI GCSGC 3471 3468 . . 3468 3472 + BlsI GCNGC 3470 3469 . . 3468 3472 + BisI GCNGC 3469 3470 . . 3470 3473 + CviJI RGCY 3471 3471 . . 3468 3471 - AciI CCGC 3468 3470 . . 3466 3471 - BsrBI CCGCTC 3468 3468 . . 3480 3483 + TspEI AATT 3479 3483 . . 3482 3485 + MseI TTAA 3482 3484 . . 3490 3494 + TspDTI ATGAA 3505 3503 . . 3494 3498 + TspDTI ATGAA 3509 3507 . . 3502 3506 + Tsp45I GTSAC 3501 3506 . . 3502 3506 + MaeIII GTNAC 3501 3506 . . 3523 3526 + SetI ASST 3526 3522 . . 3525 3530 + SalI GTCGAC 3525 3529 . . 3525 3530 + Hpy166II GTNNAC 3527 3527 . . 3525 3530 + HindII GTYRAC 3527 3527 . . 3525 3530 + AccI GTMKAC 3526 3528 . . 3526 3529 + TaqI TCGA 3526 3528 . . 3529 3533 + Tsp4CI* ACNGT 3531 3530 . . 3532 3536 + MaeIII GTNAC 3531 3536 . . 3546 3551 + XhoII RGATCY 3546 3550 . . 3546 3551 + BglII AGATCT 3546 3550 . . 3547 3550 + DpnI GATC 3548 3548 . . 3547 3550 + BstKTI GATC 3549 3547 . . 3547 3550 + MboI GATC 3546 3550 . . 3549 3554 + XbaI TCTAGA 3549 3553 . . 3549 3554 + Hpy178III* TCNNGA 3550 3552 . . 3550 3553 + MaeI CTAG 3550 3552 . . 3553 3563 + Hin4I GAYNNNNNVTC 3544 3539 3576 3571 3562 3565 + TaqI TCGA 3562 3564 . . 3553 3563 - Hin4I GAYNNNNNVTC 3576 3571 3544 3539 3564 3568 + PleI GAGTC 3572 3573 . . 3564 3568 + MlyI GAGTC 3573 3573 . . 3564 3568 + HinfI GANTC 3564 3567 . . 3566 3571 + Hpy166II GTNNAC 3568 3568 . . 3566 3571 + HindII GTYRAC 3568 3568 . . 3574 3578 + Hpy188I TCNGA 3576 3575 . . 3582 3586 + BslFI GGGAC 3596 3600 . . 3586 3589 + MaeI CTAG 3586 3588 . . 3598 3602 + HphI GGTGA 3610 3609 . . 3601 3605 + MboII GAAGA 3613 3612 . . 3601 3606 + BbvII* GAAGAC 3608 3612 . . 3604 3608 + HgaI GACGC 3613 3618 . . 3605 3610 + MluI ACGCGT 3605 3609 . . 3605 3610 + AflIII ACRYGT 3605 3609 . . 3606 3609 + FnuDII* CGCG 3607 3607 . . 3609 3612 + Csp6I GTAC 3609 3611 . . 3609 3612 + RsaI GTAC 3610 3610 . . 3617 3620 + SetI ASST 3620 3616 . . 3622 3625 + MseI TTAA 3622 3624 . . 3628 3632 + DdeI CTNAG 3628 3631 . . 3628 3632 - BspCNI CTCAG 3620 3618 . . 3628 3632 - BseMII CTCAG 3619 3617 . . 3637 3640 + SetI ASST 3640 3636 . . 3635 3639 - Hin4II* CCTTC 3629 3628 . . 3643 3648 + VspI ATTAAT 3644 3646 . . 3644 3647 + MseI TTAA 3644 3646 . . 3651 3656 + XhoII RGATCY 3651 3655 . . 3651 3655 + BinI* GGATC 3659 3660 . . 3652 3655 + DpnI GATC 3653 3653 . . 3652 3655 + BstKTI GATC 3654 3652 . . 3652 3655 + MboI GATC 3651 3655 . . 3654 3659 + Hpy178III* TCNNGA 3655 3657 . . 3664 3670 + TspRI CASTGNN 3670 3661 . . 3673 3677 + MboII GAAGA 3685 3684 . . 3673 3678 - Ksp632I* CTCTTC 3668 3671 . . 3684 3688 + TauI GCSGC 3687 3684 . . 3684 3688 + BlsI GCNGC 3686 3685 . . 3684 3688 + BisI GCNGC 3685 3686 . . 3687 3692 + Cac8I GCNNGC 3689 3689 . . 3684 3687 - AciI CCGC 3684 3686 . . 3689 3694 + HindIII AAGCTT 3689 3693 . . 3690 3693 + SetI ASST 3693 3689 . . 3690 3693 + CviJI RGCY 3691 3691 . . 3690 3693 + AluI AGCT 3691 3691 . . 3708 3715 + PmeI GTTTAAAC 3711 3711 . . 3709 3714 + AhaIII* TTTAAA 3711 3711 . . 3710 3713 + MseI TTAA 3710 3712 . . 3722 3727 + MfeI CAATTG 3722 3726 . . 3723 3726 + TspEI AATT 3722 3726 . . 3733 3737 + Tsp45I GTSAC 3732 3737 . . 3733 3737 + MaeIII GTNAC 3732 3737 . . 3735 3740 + TaqII CACCCA 3751 3749 . . 3734 3738 - HphI GGTGA 3726 3725 . . 3738 3748 + BsiYI* CCNNNNNNNGG 3744 3741 . . 3738 3748 + PflMI CCANNNNNTGG 3744 3741 . . 3753 3758 + NspI RCATGY 3757 3753 . . 3753 3758 + Cac8I GCNNGC 3755 3755 . . 3753 3758 + SphI GCATGC 3757 3753 . . 3754 3757 + FatI CATG 3753 3757 . . 3754 3757 + NlaIII CATG 3757 3753 . . 3754 3757 + CviAII CATG 3754 3756 . . 3763 3766 + SetI ASST 3766 3762 . . 3775 3779 + HgaI GACGC 3784 3789 . . 3778 3788 + MwoI GCNNNNNNNGC 3784 3781 . . 3787 3790 - AciI CCGC 3787 3789 . . 3798 3801 + CviJI RGCY 3799 3799 . . 3809 3812 + MseI TTAA 3809 3811 . . 3824 3827 + MnlI CCTC 3834 3833 . . 3831 3836 + BsiI* CACGAG 3831 3835 . . 3843 3847 + BmgT120I GGNCC 3844 3845 . . 3843 3847 + AvaII GGWCC 3843 3846 . . 3843 3847 + AsuI* GGNCC 3843 3846 . . 3840 3844 - Hin4II* CCTTC 3834 3833 . . 3849 3852 + TspEI AATT 3848 3852 . . 3860 3865 + Cac8I GCNNGC 3862 3862 . . 3863 3866 + CviRI* TGCA 3864 3864 . . 3866 3869 + SetI ASST 3869 3865 . . 3864 3869 - BspMI ACCTGC 3855 3859 . . 3870 3873 + Hin6I GCGC 3870 3872 . . 3870 3873 + GlaI GCGC 3871 3871 . . 3870 3873 + HhaI GCGC 3872 3870 . . 3864 3870 - AarI CACCTGC 3855 3859 . . 3877 3882 + NdeI CATATG 3878 3880 . . 3883 3888 + MmeI TCCRAC 3908 3906 . . 3929 3932 + CviRI* TGCA 3930 3930 . . 3930 3935 + NspI RCATGY 3934 3930 . . 3931 3934 + FatI CATG 3930 3934 . . 3931 3934 + NlaIII CATG 3934 3930 . . 3931 3934 + CviAII CATG 3931 3933 . . 3951 3954 + CviRI* TGCA 3952 3952 . . 3975 3980 + NlaIV GGNNCC 3977 3977 . . 3991 3995 + MboII GAAGA 4003 4002 . . 4004 4009 + SspI AATATT 4006 4006 . . 4012 4016 + MaeIII GTNAC 4011 4016 . . 4035 4040 + MmeI TCCRAC 4060 4058 . . 4036 4046 + BsiYI* CCNNNNNNNGG 4042 4039 . . 4033 4037 - FokI GGATG 4019 4023 . . 4033 4037 - BseGI GGATG 4032 4030 . . 4042 4051 + XmnI GAANNNNTTC 4046 4046 . . 4042 4046 - Hin4II* CCTTC 4036 4035 . . 4046 4051 - BsmI GAATGC 4046 4044 . . 4051 4054 + AciI CCGC 4051 4053 . . 4054 4059 + BsiI* CACGAG 4054 4058 . . 4063 4068 + Eco57MI CTGRAG 4084 4082 . . 4063 4068 + Eco57I CTGAAG 4084 4082 . . 4065 4069 + MboII GAAGA 4077 4076 . . 4085 4088 + CviJI RGCY 4086 4086 . . 4091 4094 + TaqI TCGA 4091 4093 . . 4093 4098 + ApoI RAATTY 4093 4097 . . 4094 4097 + TspEI AATT 4093 4097 . . 4111 4115 - BccI CCATC 4105 4106 . . 4121 4124 + SetI ASST 4124 4120 . . 4120 4123 - MnlI CCTC 4113 4112 . . 4129 4134 + BseRI GAGGAG 4144 4142 . . 4129 4132 - MnlI CCTC 4122 4121 . . 4148 4152 + SfaNI GCATC 4157 4161 . . 4144 4148 - Hin4II* CCTTC 4138 4137 . . 4159 4162 + TaqI TCGA 4159 4161 . . 4170 4173 + TspEI AATT 4169 4173 . . 4172 4175 + MseI TTAA 4172 4174 . . 4175 4180 + XhoII RGATCY 4175 4179 . . 4176 4179 + DpnI GATC 4177 4177 . . 4176 4179 + BstKTI GATC 4178 4176 . . 4176 4179 + MboI GATC 4175 4179 . . 4176 4180 - BinI* GGATC 4170 4171 . . 4181 4184 + CviJI RGCY 4182 4182 . . 4189 4192 + SetI ASST 4192 4188 . . 4199 4202 + CviRI* TGCA 4200 4200 . . 4239 4242 + CviJI RGCY 4240 4240 . . 4269 4280 + BdaI TGANNNNNNTCA 4258 4256 4292 4290 4269 4280 - BdaI TGANNNNNNTCA 4292 4290 4258 4256 4286 4295 + XmnI GAANNNNTTC 4290 4290 . . 4295 4299 + DdeI CTNAG 4295 4298 . . 4295 4299 + BspCNI CTCAG 4308 4306 . . 4295 4299 + BseMII CTCAG 4309 4307 . . 4296 4300 + Hpy188I TCNGA 4298 4297 . . 4299 4303 + MboII GAAGA 4311 4310 . . 4302 4306 - Hin4II* CCTTC 4296 4295 . . 4311 4316 + EcoRV GATATC 4313 4313 . . 4306 4316 - Hin4I GAYNNNNNVTC 4329 4324 4297 4292 4318 4323 + ApoI RAATTY 4318 4322 . . 4319 4322 + TspEI AATT 4318 4322 . . 4323 4328 + XbaI TCTAGA 4323 4327 . . 4323 4328 + Hpy178III* TCNNGA 4324 4326 . . 4324 4327 + MaeI CTAG 4324 4326 . . 4328 4332 + Tsp4CI* ACNGT 4330 4329 . . 4331 4336 + BsgI GTGCAG 4352 4350 . . 4332 4335 + CviRI* TGCA 4333 4333 . . 4351 4354 + TspEI AATT 4350 4354 . . 4362 4365 + CviJI RGCY 4363 4363 . . 4360 4363 - MnlI CCTC 4353 4352 . . 4370 4373 + TspEI AATT 4369 4373 . . 4372 4375 + MseI TTAA 4372 4374 . . 4379 4382 + CviRI* TGCA 4380 4380 . . 4397 4400 + SetI ASST 4400 4396 . . 4434 4438 + BssKI CCNGG 4433 4438 . . 4434 4438 + BseBI CCWGG 4435 4436 . . 4434 4439 + SecI* CCNNGG 4434 4438 . . 4434 4438 + ScrFI CCNGG 4435 4436 . . 4434 4438 + EcoRII CCWGG 4433 4438 . . 4461 4464 + SetI ASST 4464 4460 . . 4467 4470 + TspEI AATT 4466 4470 . . 4486 4490 + Hpy188I TCNGA 4488 4487 . . 4485 4490 - MmeI TCCRAC 4466 4464 . . 4492 4495 + TspEI AATT 4491 4495 . . 4501 4504 + SetI ASST 4504 4500 . . 4501 4504 + CviJI RGCY 4502 4502 . . 4501 4504 + AluI AGCT 4502 4502 . . 4509 4512 + CviRI* TGCA 4510 4510 . . 4514 4519 + Hpy166II GTNNAC 4516 4516 . . 4515 4520 + TatI WGTACW 4515 4519 . . 4516 4519 + Csp6I GTAC 4516 4518 . . 4516 4519 + RsaI GTAC 4517 4517 . . 4521 4526 + Cac8I GCNNGC 4523 4523 . . 4524 4527 + CviRI* TGCA 4525 4525 . . 4525 4529 + SfaNI GCATC 4534 4538 . . 4526 4530 - FokI GGATG 4512 4516 . . 4526 4530 - BseGI GGATG 4525 4523 . . 4534 4544 + BaeI ACNNNNGTAYC 4523 4518 4556 4551 4537 4541 + Tsp4CI* ACNGT 4539 4538 . . 4540 4543 + Csp6I GTAC 4540 4542 . . 4540 4543 + RsaI GTAC 4541 4541 . . 4567 4571 + BsrI ACTGG 4572 4570 . . 4567 4572 + BfiI ACTGGG 4577 4576 . . 4579 4583 + TfiI GAWTC 4579 4582 . . 4579 4583 + HinfI GANTC 4579 4582 . . 4585 4589 + Hpy188I TCNGA 4587 4586 . . 4582 4586 - MboII GAAGA 4574 4573 . . 4583 4588 - Eco57MI CTGRAG 4568 4566 . . 4583 4588 - Eco57I CTGAAG 4568 4566 . . 4596 4599 + CviJI RGCY 4597 4597 . . 4598 4602 + DdeI CTNAG 4598 4601 . . 4602 4606 + TfiI GAWTC 4602 4605 . . 4602 4606 + HinfI GANTC 4602 4605 . . #--------------------------------------- # # # AarI: # [CACCTGC] # 756 1431 2424 # # AccI: # [GTMKAC] # 487 1085 1346 1693 # # AciI: # [CCGC] # 103 138 216 264 337 404 # 560 1276 1313 # # AclI: # [AACGTT] # 1208 3403 # # AflIII: # [ACRYGT] # 489 1006 3116 # # AhaIII*: # [TTTAAA] # 258 481 756 900 906 1310 # # AjuI: # [GAANNNNNNNTTGG] # 32 32 580 1029 2938 # # AloI: # [GAACNNNNNNTCC] # 32 32 32 83 883 1581 # 1968 # # AluI: # [AGCT] # 16 39 48 63 67 69 # 81 109 133 188 360 382 # 506 654 811 1085 # # AlwNI: # [CAGNNNCTG] # 1429 3182 # # ApoI: # [RAATTY] # 7 24 73 186 225 293 # 402 419 466 525 597 655 # 739 # # AsuI*: # [GGNCC] # 203 259 310 376 673 768 # 916 1106 # # AsuII: # [TTCGAA] # 22 1071 1534 1984 # # AvaII: # [GGWCC] # 376 768 1568 1899 # # BaeI: # [ACNNNNGTAYC] # 33 55 4523 # # BalI: # [TGGCCA] # 203 2044 2364 # # BbvI: # [GCAGC] # 3 26 69 144 300 351 # 967 1010 1741 # # BbvII*: # [GAAGAC] # 481 1003 3127 # # BccI: # [CCATC] # 40 108 136 241 506 598 # 861 957 1164 # # Bce83I*: # [CTTGAG] # 186 320 666 891 2548 # # BceAI: # [ACGGC] # 532 1067 3012 # # BcgI: # [CGANNNNNNTGC] # 34 619 3958 # # BciVI: # [GTATCC] # 767 807 3037 # # BclI: # [TGATCA] # 1740 2871 # # BdaI: # [TGANNNNNNTCA] # 0 0 0 0 34 34 # 319 1660 2564 # # BetI*: # [WCCGGW] # 171 714 1023 2703 # # BfiI: # [ACTGGG] # 34 691 1218 2668 # # BglII: # [AGATCT] # 1065 1755 1791 # # BinI*: # [GGATC] # 227 441 511 518 1223 1691 # # BisI: # [GCNGC] # 3 26 94 133 149 177 # 216 300 610 926 967 1010 # # BlsI: # [GCNGC] # 3 26 94 134 149 177 # 216 300 610 925 967 1010 # # BmgT120I: # [GGNCC] # 203 259 310 377 673 767 # 916 1106 # # BplI: # [GAGNNNNNCTC] # 0 0 32 1873 2706 # # Bpu10I: # [CCTNAGC] # 6 787 1508 2310 # # BsaAI: # [YACGTR] # 2153 2458 # # BsaBI: # [GATNNNNATC] # 1922 2689 # # BsaXI: # [ACNNNNNCTCC] # 30 83 4498 # # BseBI: # [CCWGG] # 103 176 1877 2455 # # BseGI: # [GGATG] # 36 86 303 493 612 672 # 729 1680 # # BseMII: # [CTCAG] # 6 54 90 201 228 276 # 302 421 690 693 775 875 # # BseRI: # [GAGGAG] # 467 825 867 2452 # # BseYI: # [CCCAGC] # 1348 3263 # # BsgI: # [GTGCAG] # 259 562 571 3219 # # BsiI*: # [CACGAG] # 223 307 557 3524 # # BsiYI*: # [CCNNNNNNNGG] # 1 24 57 107 154 290 # 298 396 569 648 886 1181 # # BslFI: # [GGGAC] # 81 1015 1666 1849 # # BsmAI: # [GTCTC] # 1808 2803 # # BsmI: # [GAATGC] # 375 502 565 3169 # # BspCNI: # [CTCAG] # 6 54 90 203 230 274 # 303 423 688 693 774 873 # # BspHI: # [TCATGA] # 2211 2400 # # BspMI: # [ACCTGC] # 756 1431 2424 # # BspMII*: # [TCCGGA] # 171 714 3726 # # BsrBI: # [CCGCTC] # 1143 3468 # # BsrI: # [ACTGG] # 20 39 225 271 407 435 # 1218 1996 # # BssKI: # [CCNGG] # 13 101 178 663 1779 1877 # # BssNAI: # [GTATAC] # 1834 2777 # # BstAPI: # [GCANNNNNTGC] # 1620 2991 # # BstKTI: # [GATC] # 44 64 84 87 105 132 # 168 262 274 335 413 433 # 524 1686 # # BtgZI: # [GCGATG] # 122 1016 3473 # # BtsI: # [GCAGTG] # 1942 2669 # # Cac8I: # [GCNNGC] # 66 88 107 345 557 591 # 661 2196 # # CauII*: # [CCSGG] # 116 663 3832 # # CfrI: # [YGGCCR] # 201 981 1383 2046 # # ClaI: # [ATCGAT] # 1291 1594 1726 # # Csp6I: # [GTAC] # 24 42 65 71 87 170 # 255 510 704 750 907 1026 # # CspCI: # [CAANNNNNGTGG] # 23 35 4553 # # CviAII: # [CATG] # 5 12 15 15 18 26 # 77 80 130 146 177 177 # 223 363 499 680 864 1104 # # CviJI: # [RGCY] # 6 7 7 8 9 14 # 14 16 16 17 21 24 # 27 28 36 38 39 46 # 48 48 51 51 52 56 # 57 57 57 58 68 68 # 68 73 79 81 82 89 # 92 94 94 94 95 96 # 104 106 108 123 126 126 # 139 160 163 170 174 219 # 220 287 305 # # CviRI*: # [TGCA] # 4 13 15 22 22 37 # 45 47 66 86 130 133 # 177 203 248 250 280 296 # 384 495 707 951 # # DdeI: # [CTNAG] # 6 13 54 90 163 173 # 224 251 253 255 281 303 # 438 588 667 852 # # DpnI: # [GATC] # 44 64 84 87 105 132 # 168 262 274 335 413 434 # 524 1685 # # DraII: # [RGGNCCY] # 1441 3170 # # DsaI*: # [CCRYGG] # 1271 3340 # # Ecl136II: # [GAGCTC] # 593 1296 2722 # # Eco57I: # [CTGAAG] # 43 475 484 931 2678 # # Eco57MI: # [CTGRAG] # 43 85 390 484 931 1134 # 1544 # # EcoNI: # [CCTNNNNNAGG] # 685 943 2983 # # EcoP15I: # [CAGCAG] # 3 160 369 405 939 2735 # # EcoRI: # [GAATTC] # 24 4587 # # EcoRII: # [CCWGG] # 101 178 1877 2455 # # EcoRV: # [GATATC] # 115 259 298 720 950 1093 # 1176 # # EcoT22I: # [ATGCAT] # 1493 3118 # # Esp3I: # [CGTCTC] # 1808 2803 # # EspI*: # [GCTNAGC] # 1454 3157 # # FalI: # [AAGNNNNNCTT] # 0 0 0 0 0 0 # 0 0 32 32 32 32 # 167 200 647 1161 2308 # # FatI: # [CATG] # 5 12 15 15 18 26 # 77 80 130 146 177 177 # 222 363 499 681 864 1104 # # FauI: # [CCCGC] # 549 1313 2749 # # FnuDII*: # [CGCG] # 711 1004 2896 # # FokI: # [GGATG] # 16 99 303 493 592 679 # 749 1680 # # GlaI: # [GCGC] # 740 3871 # # GsaI: # [CCCAGC] # 1344 3267 # # GsuI: # [CTGGAG] # 85 2071 2455 # # HaeIII: # [GGCC] # 103 157 203 241 271 312 # 316 604 964 1440 # # HgaI: # [GACGC] # 67 171 181 452 668 827 # 2245 # # HgiAI*: # [GWGCWC] # 67 593 1294 2657 # # HgiCI*: # [GGYRCC] # 1041 3570 # # HgiJII*: # [GRGCYC] # 593 760 1294 1964 # # HhaI: # [GCGC] # 739 3872 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 0 0 # 13 32 32 32 32 32 # 32 32 32 52 191 267 # 282 308 435 721 2086 # # Hin4II*: # [CCTTC] # 78 84 102 158 202 205 # 234 314 315 468 657 872 # 922 # # Hin6I: # [GCGC] # 741 3870 # # HindII: # [GTYRAC] # 41 627 1043 2900 # # HindIII: # [AAGCTT] # 922 1635 2054 # # HinfI: # [GANTC] # 9 23 33 38 90 93 # 193 440 542 551 627 957 # 1015 # # HpaI: # [GTTAAC] # 1711 2900 # # HpaII: # [CCGG] # 63 108 116 495 528 599 # 2702 # # HphI: # [GGTGA] # 116 307 885 1596 1707 # # Hpy166II: # [GTNNAC] # 41 95 187 227 293 334 # 487 933 948 1066 # # Hpy178III*: # [TCNNGA] # 13 15 20 30 46 47 # 71 84 87 90 104 105 # 105 108 108 117 119 171 # 195 280 287 382 399 422 # 537 669 # # Hpy188I: # [TCNGA] # 15 24 99 135 166 190 # 254 273 303 314 579 722 # 726 811 # # Hpy99I: # [CGWCG] # 513 4098 # # Ksp632I*: # [CTCTTC] # 145 162 183 286 363 448 # 943 1001 1080 # # MaeI: # [CTAG] # 36 99 131 287 422 439 # 575 684 738 1200 # # MaeII: # [ACGT] # 33 351 389 549 560 575 # 659 1495 # # MaeIII: # [GTNAC] # 2 21 30 201 265 279 # 383 453 600 639 837 901 # # MboI: # [GATC] # 44 64 84 87 105 132 # 168 262 274 335 413 436 # 524 1683 # # MboII: # [GAAGA] # 3 3 7 7 9 10 # 15 24 27 36 37 60 # 72 74 77 82 101 123 # 135 149 193 201 234 263 # 276 276 318 371 388 457 # 583 # # McrI*: # [CGRYCG] # 66 1664 2881 # # MfeI: # [CAATTG] # 275 889 1224 2223 # # MluI: # [ACGCGT] # 1006 3605 # # MlyI: # [GAGTC] # 1038 1411 2162 # # MmeI: # [TCCRAC] # 95 145 152 406 656 1577 # 1580 # # MnlI: # [CCTC] # 3 9 9 9 19 21 # 23 24 25 29 29 40 # 40 47 53 57 62 64 # 71 71 83 83 86 90 # 109 114 121 132 136 137 # 138 142 177 190 226 231 # 258 277 279 420 477 # # MseI: # [TTAA] # 7 9 22 23 66 72 # 73 75 80 99 105 140 # 146 200 239 363 408 489 # 508 732 755 # # MslI: # [CAYNNNNRTG] # 157 260 330 1700 2164 # # MwoI: # [GCNNNNNNNGC] # 32 37 234 379 510 662 # 814 827 1116 # # NdeI: # [CATATG] # 733 3878 # # NlaIII: # [CATG] # 5 12 15 15 18 26 # 77 80 130 146 177 177 # 226 363 499 677 864 1104 # # NlaIV: # [GGNNCC] # 68 69 187 333 573 634 # 903 1844 # # NspBII*: # [CMGCKG] # 544 4067 # # NspI: # [RCATGY] # 12 30 177 203 677 1453 # 2059 # # PflMI: # [CCANNNNNTGG] # 867 1181 2563 # # PleI: # [GAGTC] # 1039 1411 2161 # # PmeI: # [GTTTAAAC] # 900 3711 # # PpiI: # [GAACNNNNNCTC] # 32 83 4496 # # PsiI: # [TTATAA] # 398 482 1625 2106 # # PstI: # [CTGCAG] # 1947 2664 # # PvuI: # [CGATCG] # 1730 2881 # # RsaI: # [GTAC] # 24 42 65 70 87 171 # 255 510 704 750 907 1026 # # SacI: # [GAGCTC] # 593 1294 2724 # # SalI: # [GTCGAC] # 1086 3525 # # SapI: # [GCTCTTC] # 593 1306 2712 # # ScaI: # [AGTACT] # 171 4440 # # ScrFI: # [CCNGG] # 13 103 176 663 1779 1877 # # SduI: # [GDGCHC] # 67 593 693 1294 1964 # # SecI*: # [CCNNGG] # 102 177 744 753 1094 1741 # # SetI: # [ASST] # 4 6 8 9 11 15 # 16 17 18 19 20 21 # 23 23 25 29 32 33 # 34 34 38 38 39 40 # 40 53 64 64 68 69 # 71 73 94 94 103 107 # 111 124 130 141 144 146 # 147 150 152 193 201 208 # 219 232 255 255 351 # # SfaNI: # [GCATC] # 30 30 45 77 186 225 # 239 296 303 377 552 991 # 1260 # # SfeI*: # [CTRYAG] # 216 243 645 767 789 1951 # # SmlI: # [CTYRAG] # 165 356 666 891 2533 # # SphI: # [GCATGC] # 854 1495 2262 # # SspI: # [AATATT] # 577 605 1224 2205 # # StyI: # [CCWWGG] # 855 3756 # # TaiI: # [ACGT] # 33 351 389 549 560 575 # 662 1492 # # TaqI: # [TCGA] # 9 22 36 39 66 68 # 84 117 169 179 186 192 # 206 231 234 241 262 399 # 443 447 452 529 # # TaqII: # [CACCCA] # 860 3751 # # TatI: # [WGTACW] # 96 169 1026 1417 1903 # # TauI: # [GCSGC] # 216 406 924 3065 # # TfiI: # [GAWTC] # 9 23 33 38 90 93 # 193 542 627 1455 1508 # # TseI: # [GCWGC] # 3 26 94 132 300 326 # 967 1010 1753 # # TsoI: # [TARCCA] # 34 447 724 1278 2128 # # Tsp45I: # [GTSAC] # 2 231 286 383 639 837 # 879 1354 # # Tsp4CI*: # [ACNGT] # 8 72 95 152 209 301 # 534 727 799 1714 # # TspDTI: # [ATGAA] # 4 16 28 52 66 74 # 78 86 113 167 192 199 # 221 382 413 493 925 1102 # # TspEI: # [AATT] # 6 6 7 18 19 25 # 26 29 31 32 43 67 # 73 76 92 95 97 120 # 125 126 147 149 150 155 # 243 245 251 280 306 346 # 358 402 466 # # TspGWI: # [ACGGA] # 104 153 829 1178 2347 # # TspRI: # [CASTGNN] # 90 147 230 401 617 771 # 941 1414 # # TstI: # [CACNNNNNNTCC] # 32 2277 2302 # # VspI: # [ATTAAT] # 967 3644 # # XbaI: # [TCTAGA] # 288 774 3549 # # XcmI: # [CCANNNNNNNNNTGG] # 1035 3576 # # XhoII: # [RGATCY] # 105 132 413 436 524 1210 # 1791 # # XmnI: # [GAANNNNTTC] # 192 244 321 3854 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 4611 # Reported_sequences: 1 # Reported_hitcount: 1058 #---------------------------------------