Restriction Fragments for PHO80/YOL001W



Send questions or suggestions to SGD

BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

PHO80/YOL001W on chromosome XV from coordinates 325249 to 326130.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.28045.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 13 18 + Hpy178III* TCNNGA 14 16 . . 20 25 + AclI AACGTT 21 23 . . 21 24 + TaiI ACGT 24 20 . . 21 24 + SetI ASST 24 20 . . 21 24 + MaeII ACGT 21 23 . . 25 29 + Hpy188I TCNGA 27 26 . . 37 40 + FatI CATG 36 40 . . 37 40 + NlaIII CATG 40 36 . . 37 40 + CviAII CATG 37 39 . . 42 46 + BinI* GGATC 50 51 . . 40 43 - MnlI CCTC 33 32 . . 43 46 + DpnI GATC 44 44 . . 43 46 + BstKTI GATC 45 43 . . 43 46 + MboI GATC 42 46 . . 50 55 - BciVI GTATCC 44 43 . . 63 66 + TspEI AATT 62 66 . . 69 74 + Cac8I GCNNGC 71 71 . . 70 74 + FauI CCCGC 78 80 . . 71 76 + NspBII* CMGCKG 73 73 . . 71 74 + AciI CCGC 71 73 . . 80 83 + MseI TTAA 80 82 . . 91 96 + XbaI TCTAGA 91 95 . . 91 96 + Hpy178III* TCNNGA 92 94 . . 92 95 + MaeI CTAG 92 94 . . 101 104 + SetI ASST 104 100 . . 103 106 + MaeI CTAG 103 105 . . 109 114 + SduI GDGCHC 113 109 . . 109 114 + HgiAI* GWGCWC 113 109 . . 118 123 + Hpy178III* TCNNGA 119 121 . . 137 148 + BdaI TGANNNNNNTCA 126 124 160 158 138 147 + BsaBI GATNNNNATC 142 142 . . 137 148 - BdaI TGANNNNNNTCA 160 158 126 124 149 153 + TspDTI ATGAA 164 162 . . 153 158 + ApoI RAATTY 153 157 . . 154 157 + TspEI AATT 153 157 . . 172 176 + Hpy188I TCNGA 174 173 . . 183 186 + TspEI AATT 182 186 . . 190 193 + MseI TTAA 190 192 . . 206 210 + DdeI CTNAG 206 209 . . 210 214 + TfiI GAWTC 210 213 . . 210 214 + HinfI GANTC 210 213 . . 214 217 + MnlI CCTC 224 223 . . 230 234 - MboII GAAGA 222 221 . . 241 245 - TspDTI ATGAA 231 229 . . 263 266 + MnlI CCTC 273 272 . . 304 309 + ClaI ATCGAT 305 307 . . 305 308 + TaqI TCGA 305 307 . . 314 317 + CviRI* TGCA 315 315 . . 319 323 + Tsp4CI* ACNGT 321 320 . . 324 329 + BciVI GTATCC 335 334 . . 327 332 + Hpy178III* TCNNGA 328 330 . . 341 344 + MseI TTAA 341 343 . . 343 346 + TspEI AATT 342 346 . . 348 353 + Hpy166II GTNNAC 350 350 . . 348 353 + HindII GTYRAC 350 350 . . 361 364 + SetI ASST 364 360 . . 370 373 + MseI TTAA 370 372 . . 375 378 + CviJI RGCY 376 376 . . 382 386 + Tsp4CI* ACNGT 384 383 . . 397 400 + CviJI RGCY 398 398 . . 406 410 + TfiI GAWTC 406 409 . . 406 410 + HinfI GANTC 406 409 . . 406 416 + Hin4I GAYNNNNNVTC 397 392 429 424 424 434 + MwoI GCNNNNNNNGC 430 427 . . 432 435 + CviRI* TGCA 433 433 . . 443 448 + BseRI GAGGAG 458 456 . . 439 444 - MmeI TCCRAC 420 418 . . 443 446 - MnlI CCTC 436 435 . . 448 451 + Csp6I GTAC 448 450 . . 448 451 + RsaI GTAC 449 449 . . 455 459 + Tsp45I GTSAC 454 459 . . 455 459 + MaeIII GTNAC 454 459 . . 456 461 + Hpy178III* TCNNGA 457 459 . . 461 464 + TspEI AATT 460 464 . . 471 475 + BsrI ACTGG 476 474 . . 472 477 + Eco57MI CTGRAG 493 491 . . 472 477 + GsuI CTGGAG 493 491 . . 486 491 + AhaIII* TTTAAA 488 488 . . 487 490 + MseI TTAA 487 489 . . 496 501 + Hpy166II GTNNAC 498 498 . . 501 506 + SfeI* CTRYAG 501 505 . . 506 510 + TfiI GAWTC 506 509 . . 506 510 + HinfI GANTC 506 509 . . 514 519 + NspBII* CMGCKG 516 516 . . 514 519 + SacII CCGCGG 517 515 . . 514 519 + DsaI* CCRYGG 514 518 . . 514 519 + SecI* CCNNGG 514 518 . . 514 517 + AciI CCGC 514 516 . . 515 518 + FnuDII* CGCG 516 516 . . 519 523 + BinI* GGATC 527 528 . . 516 519 - AciI CCGC 516 518 . . 516 520 - FauI CCCGC 509 511 . . 520 523 + DpnI GATC 521 521 . . 520 523 + BstKTI GATC 522 520 . . 520 523 + MboI GATC 519 523 . . 532 535 + TaiI ACGT 535 531 . . 532 535 + SetI ASST 535 531 . . 532 535 + MaeII ACGT 532 534 . . 590 595 + Eco31I GGTCTC 596 600 . . 591 595 + BsmAI GTCTC 596 600 . . 594 599 + Hpy178III* TCNNGA 595 597 . . 596 599 + TaqI TCGA 596 598 . . 604 608 + TfiI GAWTC 604 607 . . 604 608 + HinfI GANTC 604 607 . . 617 620 + TaiI ACGT 620 616 . . 617 620 + SetI ASST 620 616 . . 617 620 + MaeII ACGT 617 619 . . 620 623 + MseI TTAA 620 622 . . 628 638 + BsiYI* CCNNNNNNNGG 634 631 . . 628 638 + PflMI CCANNNNNTGG 634 631 . . 648 653 + XbaI TCTAGA 648 652 . . 648 653 + Hpy178III* TCNNGA 649 651 . . 649 652 + MaeI CTAG 649 651 . . 637 649 - AloI GAACNNNNNNTCC 661 656 629 624 663 666 + TaqI TCGA 663 665 . . 665 669 + MboII GAAGA 677 676 . . 676 681 + PvuII CAGCTG 678 678 . . 676 681 + NspBII* CMGCKG 678 678 . . 677 680 + SetI ASST 680 676 . . 677 680 + CviJI RGCY 678 678 . . 677 680 + AluI AGCT 678 678 . . 685 690 + NlaIV GGNNCC 687 687 . . 692 695 + MseI TTAA 692 694 . . 702 705 + SetI ASST 705 701 . . 700 704 - HphI GGTGA 692 691 . . 719 722 + SetI ASST 722 718 . . 735 739 + FauI CCCGC 743 745 . . 736 739 + AciI CCGC 736 738 . . 742 747 + SspI AATATT 744 744 . . 766 769 + CviJI RGCY 767 767 . . 766 771 + NlaIV GGNNCC 768 768 . . 800 805 + TaqII CACCCA 816 814 . . 799 803 - HphI GGTGA 791 790 . . 805 808 + TspEI AATT 804 808 . . 813 818 + Ksp632I* CTCTTC 819 822 . . 814 818 - MboII GAAGA 806 805 . . 832 836 + Hpy188I TCNGA 834 833 . . 833 837 + DdeI CTNAG 833 836 . . 833 837 - BspCNI CTCAG 825 823 . . 833 837 - BseMII CTCAG 824 822 . . 835 838 - MnlI CCTC 828 827 . . 838 848 + MwoI GCNNNNNNNGC 844 841 . . 844 848 + HgaI GACGC 853 858 . . 864 874 + MwoI GCNNNNNNNGC 870 867 . . 866 869 + DpnI GATC 867 867 . . 866 869 + BstKTI GATC 868 866 . . 866 869 + MboI GATC 865 869 . . 872 875 + CviJI RGCY 873 873 . . 879 882 + MseI TTAA 879 881 . . #--------------------------------------- # # # AciI: # [CCGC] # 2 71 146 220 443 # # AclI: # [AACGTT] # 21 861 # # AhaIII*: # [TTTAAA] # 394 488 # # AloI: # [GAACNNNNNNTCC] # 32 221 629 # # AluI: # [AGCT] # 204 678 # # ApoI: # [RAATTY] # 153 729 # # BciVI: # [GTATCC] # 44 291 547 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 126 722 # # BinI*: # [GGATC] # 50 355 477 # # BsaBI: # [GATNNNNATC] # 142 740 # # BseMII: # [CTCAG] # 58 824 # # BseRI: # [GAGGAG] # 424 458 # # BsiYI*: # [CCNNNNNNNGG] # 248 634 # # BsmAI: # [GTCTC] # 286 596 # # BspCNI: # [CTCAG] # 57 825 # # BsrI: # [ACTGG] # 406 476 # # BstKTI: # [GATC] # 14 45 346 477 # # Cac8I: # [GCNNGC] # 71 811 # # ClaI: # [ATCGAT] # 305 577 # # Csp6I: # [GTAC] # 434 448 # # CviAII: # [CATG] # 37 845 # # CviJI: # [RGCY] # 9 22 89 106 280 376 # # CviRI*: # [TGCA] # 118 315 449 # # DdeI: # [CTNAG] # 49 206 627 # # DpnI: # [GATC] # 15 44 346 477 # # DsaI*: # [CCRYGG] # 368 514 # # Eco31I: # [GGTCTC] # 286 596 # # Eco57MI: # [CTGRAG] # 389 493 # # FatI: # [CATG] # 36 846 # # FauI: # [CCCGC] # 78 139 234 431 # # FnuDII*: # [CGCG] # 366 516 # # GsuI: # [CTGGAG] # 389 493 # # HgaI: # [GACGC] # 29 853 # # HgiAI*: # [GWGCWC] # 113 769 # # Hin4I: # [GAYNNNNNVTC] # 32 397 453 # # HindII: # [GTYRAC] # 350 532 # # HinfI: # [GANTC] # 98 100 196 210 278 # # HphI: # [GGTGA] # 91 99 692 # # Hpy166II: # [GTNNAC] # 148 350 384 # # Hpy178III*: # [TCNNGA] # 14 27 54 78 129 138 # 209 233 # # Hpy188I: # [TCNGA] # 27 48 147 660 # # Ksp632I*: # [CTCTTC] # 63 819 # # MaeI: # [CTAG] # 11 92 233 546 # # MaeII: # [ACGT] # 21 85 265 511 # # MaeIII: # [GTNAC] # 428 454 # # MboI: # [GATC] # 17 42 346 477 # # MboII: # [GAAGA] # 76 129 222 455 # # MmeI: # [TCCRAC] # 420 462 # # MnlI: # [CCTC] # 33 49 54 163 191 392 # # MseI: # [TTAA] # 3 29 72 80 110 117 # 133 151 187 # # MwoI: # [GCNNNNNNNGC] # 12 26 414 430 # # NlaIII: # [CATG] # 40 842 # # NlaIV: # [GGNNCC] # 81 114 687 # # NspBII*: # [CMGCKG] # 73 162 204 443 # # PflMI: # [CCANNNNNTGG] # 248 634 # # PvuII: # [CAGCTG] # 204 678 # # RsaI: # [GTAC] # 433 449 # # SacII: # [CCGCGG] # 365 517 # # SduI: # [GDGCHC] # 113 769 # # SecI*: # [CCNNGG] # 368 514 # # SetI: # [ASST] # 17 24 25 60 80 85 # 160 171 260 # # SfeI*: # [CTRYAG] # 381 501 # # SspI: # [AATATT] # 138 744 # # TaiI: # [ACGT] # 24 85 262 511 # # TaqI: # [TCGA] # 67 219 291 305 # # TaqII: # [CACCCA] # 66 816 # # TfiI: # [GAWTC] # 98 100 196 210 278 # # Tsp45I: # [GTSAC] # 428 454 # # Tsp4CI*: # [ACNGT] # 63 321 498 # # TspDTI: # [ATGAA] # 67 164 651 # # TspEI: # [AATT] # 29 62 78 91 118 160 # 344 # # XbaI: # [TCTAGA] # 91 234 557 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 882 # Reported_sequences: 1 # Reported_hitcount: 149 #---------------------------------------