Restriction Fragments for TIM23/YNR017W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

TIM23/YNR017W on chromosome XIV from coordinates 662913 to 663581.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.5818.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 8 11 + CviJI RGCY 9 9 . . 27 30 + SetI ASST 30 26 . . 40 50 + MwoI GCNNNNNNNGC 46 43 . . 37 41 - SfaNI GCATC 27 31 . . 42 47 + BsmI GAATGC 48 46 . . 46 50 + BlsI GCNGC 48 47 . . 46 50 + BisI GCNGC 47 48 . . 46 50 + TseI GCWGC 46 49 . . 50 55 + DsaI* CCRYGG 50 54 . . 50 55 + SecI* CCNNGG 50 54 . . 46 50 - BbvI GCAGC 33 37 . . 49 53 - BceAI ACGGC 34 36 . . 56 60 + TauI GCSGC 59 56 . . 56 60 + BlsI GCNGC 58 57 . . 56 60 + BisI GCNGC 57 58 . . 57 62 + CfrI YGGCCR 57 61 . . 58 61 + CviJI RGCY 59 59 . . 58 61 + HaeIII GGCC 59 59 . . 56 59 - AciI CCGC 56 58 . . 74 77 + CviJI RGCY 75 75 . . 76 82 + SauI* CCTNAGG 77 80 . . 77 81 + DdeI CTNAG 77 80 . . 105 108 + SetI ASST 108 104 . . 110 113 + TaqI TCGA 110 112 . . 113 116 + CviJI RGCY 114 114 . . 137 145 + AlwNI CAGNNNCTG 142 139 . . 138 141 + SetI ASST 141 137 . . 138 144 + PpuMI RGGWCCY 139 142 . . 138 144 + DraII RGGNCCY 139 142 . . 139 143 + BmgT120I GGNCC 140 141 . . 139 143 + AvaII GGWCC 139 142 . . 139 143 + AsuI* GGNCC 139 142 . . 142 146 + BssKI CCNGG 141 146 . . 142 146 + BseBI CCWGG 143 144 . . 142 146 + ScrFI CCNGG 143 144 . . 142 146 + EcoRII CCWGG 141 146 . . 149 154 + BsmI GAATGC 155 153 . . 151 156 + EcoT22I ATGCAT 155 151 . . 152 155 + CviRI* TGCA 153 153 . . 153 158 + NspI RCATGY 157 153 . . 154 157 + FatI CATG 153 157 . . 154 157 + NlaIII CATG 157 153 . . 154 157 + CviAII CATG 154 156 . . 157 162 + SalI GTCGAC 157 161 . . 157 162 + Hpy166II GTNNAC 159 159 . . 157 162 + HindII GTYRAC 159 159 . . 157 162 + AccI GTMKAC 158 160 . . 158 161 + TaqI TCGA 158 160 . . 164 167 + AciI CCGC 164 166 . . 167 170 + MaeI CTAG 167 169 . . 170 173 + CviJI RGCY 171 171 . . 171 175 + BlsI GCNGC 173 172 . . 171 175 + BisI GCNGC 172 173 . . 171 175 + TseI GCWGC 171 174 . . 173 176 + CviRI* TGCA 174 174 . . 174 178 + SfaNI GCATC 183 187 . . 171 175 - BbvI GCAGC 158 162 . . 178 188 + BsiYI* CCNNNNNNNGG 184 181 . . 175 179 - FokI GGATG 161 165 . . 175 179 - BseGI GGATG 174 172 . . 183 186 + CviJI RGCY 184 184 . . 189 194 + XbaI TCTAGA 189 193 . . 189 194 + Hpy178III* TCNNGA 190 192 . . 190 193 + MaeI CTAG 190 192 . . 213 218 + XhoII RGATCY 213 217 . . 213 218 + BglII AGATCT 213 217 . . 214 217 + DpnI GATC 215 215 . . 214 217 + BstKTI GATC 216 214 . . 214 217 + MboI GATC 213 217 . . 216 221 + Hpy178III* TCNNGA 217 219 . . 220 224 + MboII GAAGA 232 231 . . 223 227 + MboII GAAGA 235 234 . . 236 239 + MnlI CCTC 246 245 . . 247 250 + CviJI RGCY 248 248 . . 244 248 - Hin4II* CCTTC 238 237 . . 253 261 + AlwNI CAGNNNCTG 258 255 . . 258 262 + Hpy188I TCNGA 260 259 . . 261 264 + DpnI GATC 262 262 . . 261 264 + BstKTI GATC 263 261 . . 261 264 + MboI GATC 260 264 . . 265 269 + Hin4II* CCTTC 275 274 . . 265 275 + BsiYI* CCNNNNNNNGG 271 268 . . 261 265 - BinI* GGATC 255 256 . . 269 279 + BsiYI* CCNNNNNNNGG 275 272 . . 270 275 + DsaI* CCRYGG 270 274 . . 270 275 + SecI* CCNNGG 270 274 . . 276 281 + Hpy166II GTNNAC 278 278 . . 273 278 - TaqII CACCCA 263 261 . . 278 282 + BmgT120I GGNCC 279 280 . . 278 282 + AvaII GGWCC 278 281 . . 278 282 + AsuI* GGNCC 278 281 . . 279 284 + TaqII GACCGA 295 293 . . 287 290 + SetI ASST 290 286 . . 293 297 + MaeIII GTNAC 292 297 . . 296 300 + Tsp4CI* ACNGT 298 297 . . 298 303 + KpnI GGTACC 302 298 . . 298 303 + NlaIV GGNNCC 300 300 . . 298 303 + HgiCI* GGYRCC 298 302 . . 298 303 + Acc65I GGTACC 298 302 . . 299 302 + Csp6I GTAC 299 301 . . 299 302 + RsaI GTAC 300 300 . . 301 306 + Cfr10I RCCGGY 301 305 . . 301 306 + BetI* WCCGGW 301 305 . . 301 306 + AgeI ACCGGT 301 305 . . 302 305 + HpaII CCGG 302 304 . . 304 309 + NlaIV GGNNCC 306 306 . . 304 309 + HgiCI* GGYRCC 304 308 . . 310 315 + Hpy166II GTNNAC 312 312 . . 310 315 + AccI GTMKAC 311 313 . . 307 311 - BceAI ACGGC 292 294 . . 314 317 + SetI ASST 317 313 . . 314 319 + BspMI ACCTGC 323 327 . . 316 321 - EcoP15I CAGCAG 288 290 . . 321 325 + BslFI GGGAC 335 339 . . 323 333 + Hin4I GAYNNNNNVTC 314 309 346 341 318 323 - GsaI CCCAGC 322 318 . . 318 323 - BseYI CCCAGC 318 322 . . 332 336 + Hpy188I TCNGA 334 333 . . 323 333 - Hin4I GAYNNNNNVTC 346 341 314 309 335 338 - MnlI CCTC 328 327 . . 351 361 + Hin4I GAYNNNNNVTC 342 337 374 369 353 356 + CviRI* TGCA 354 354 . . 354 364 + MwoI GCNNNNNNNGC 360 357 . . 354 364 + BstAPI GCANNNNNTGC 360 357 . . 351 355 - SfaNI GCATC 341 345 . . 355 363 + AlwNI CAGNNNCTG 360 357 . . 361 366 + PstI CTGCAG 365 361 . . 351 361 - Hin4I GAYNNNNNVTC 374 369 342 337 361 366 + SfeI* CTRYAG 361 365 . . 362 365 + CviRI* TGCA 363 363 . . 367 372 + SspI AATATT 369 369 . . 373 376 + AciI CCGC 373 375 . . 372 377 - EciI GGCGGA 362 360 . . 384 390 + PfoI TCCNGGA 384 389 . . 385 389 + BssKI CCNGG 384 389 . . 385 389 + ScrFI CCNGG 386 387 . . 385 389 + CauII* CCSGG 386 387 . . 386 389 + HpaII CCGG 386 388 . . 383 387 - BslFI GGGAC 368 372 . . 392 395 + TspEI AATT 391 395 . . 395 398 + CviRI* TGCA 396 396 . . 397 402 + MfeI CAATTG 397 401 . . 398 401 + TspEI AATT 397 401 . . 406 410 + Tsp4CI* ACNGT 408 407 . . 410 415 + Hpy178III* TCNNGA 411 413 . . 414 418 + TfiI GAWTC 414 417 . . 414 418 + HinfI GANTC 414 417 . . 425 429 + DdeI CTNAG 425 428 . . 432 435 + SetI ASST 435 431 . . 432 438 + PpuMI RGGWCCY 433 436 . . 432 438 + DraII RGGNCCY 433 436 . . 433 438 + NlaIV GGNNCC 435 435 . . 433 437 + BmgT120I GGNCC 434 435 . . 433 437 + AvaII GGWCC 433 436 . . 433 437 + AsuI* GGNCC 433 436 . . 431 434 - MnlI CCTC 424 423 . . 436 446 + BsiYI* CCNNNNNNNGG 442 439 . . 437 441 + Hin4II* CCTTC 447 446 . . 434 438 - BslFI GGGAC 419 423 . . 441 445 + DdeI CTNAG 441 444 . . 444 447 + SetI ASST 447 443 . . 454 457 - AciI CCGC 454 456 . . 454 458 - FauI CCCGC 447 449 . . 459 463 + TfiI GAWTC 459 462 . . 459 463 + HinfI GANTC 459 462 . . 465 468 + FnuDII* CGCG 466 466 . . 472 475 + SetI ASST 475 471 . . 472 475 + CviJI RGCY 473 473 . . 472 475 + AluI AGCT 473 473 . . 487 490 + TspEI AATT 486 490 . . 501 504 + CviRI* TGCA 502 502 . . 499 503 - SfaNI GCATC 489 493 . . 505 509 + DdeI CTNAG 505 508 . . 509 512 - MnlI CCTC 502 501 . . 517 520 + FatI CATG 516 520 . . 517 520 + NlaIII CATG 520 516 . . 517 520 + CviAII CATG 517 519 . . 524 529 + NspBII* CMGCKG 526 526 . . 524 529 + SacII CCGCGG 527 525 . . 524 529 + DsaI* CCRYGG 524 528 . . 524 529 + SecI* CCNNGG 524 528 . . 524 527 + AciI CCGC 524 526 . . 525 528 + FnuDII* CGCG 526 526 . . 526 531 + Cac8I GCNNGC 528 528 . . 528 533 + SduI GDGCHC 532 528 . . 528 533 + HgiJII* GRGCYC 532 528 . . 529 532 + CviJI RGCY 530 530 . . 529 534 + NlaIV GGNNCC 531 531 . . 526 529 - AciI CCGC 526 528 . . 526 530 - FauI CCCGC 519 521 . . 530 540 + MwoI GCNNNNNNNGC 536 533 . . 538 543 + HaeII RGCGCY 542 538 . . 539 542 + Hin6I GCGC 539 541 . . 539 542 + GlaI GCGC 540 540 . . 539 542 + HhaI GCGC 541 539 . . 544 550 + DraII RGGNCCY 545 548 . . 544 549 + NlaIV GGNNCC 546 546 . . 545 551 + DraII RGGNCCY 546 549 . . 545 550 + SduI GDGCHC 549 545 . . 545 550 + Bsp120I GGGCCC 545 549 . . 545 550 + NlaIV GGNNCC 547 547 . . 545 549 + BmgT120I GGNCC 546 547 . . 545 550 + HgiJII* GRGCYC 549 545 . . 545 550 + BseSI GKGCMC 549 545 . . 545 549 + AsuI* GGNCC 545 548 . . 545 550 + ApaI GGGCCC 549 545 . . 541 546 - GsaI CCCAGC 545 541 . . 546 549 + CviJI RGCY 547 547 . . 546 549 + HaeIII GGCC 547 547 . . 541 546 - BseYI CCCAGC 541 545 . . 546 550 + BmgT120I GGNCC 547 548 . . 546 550 + AsuI* GGNCC 546 549 . . 549 552 + MnlI CCTC 559 558 . . 556 561 + HaeII RGCGCY 560 556 . . 557 560 + Hin6I GCGC 557 559 . . 557 560 + GlaI GCGC 558 558 . . 557 560 + HhaI GCGC 559 557 . . 571 575 - MboII GAAGA 563 562 . . 570 575 - BbvII* GAAGAC 563 567 . . 579 582 + SetI ASST 582 578 . . 590 595 + DsaI* CCRYGG 590 594 . . 590 595 + StyI CCWWGG 590 594 . . 590 595 + NcoI CCATGG 590 594 . . 590 595 + SecI* CCNNGG 590 594 . . 591 594 + FatI CATG 590 594 . . 591 594 + NlaIII CATG 594 590 . . 591 594 + CviAII CATG 591 593 . . 602 605 + MnlI CCTC 612 611 . . 602 607 + SecI* CCNNGG 602 606 . . 607 617 + MwoI GCNNNNNNNGC 613 610 . . 607 612 + BsrDI GCAATG 614 612 . . 603 608 - NmeAIII GCCGAG 583 581 . . 614 619 + CfrI YGGCCR 614 618 . . 615 618 + CviJI RGCY 616 616 . . 615 618 + HaeIII GGCC 616 616 . . 616 620 + TauI GCSGC 619 616 . . 616 620 + BlsI GCNGC 618 617 . . 616 620 + BisI GCNGC 617 618 . . 617 622 + NspBII* CMGCKG 619 619 . . 617 620 + AciI CCGC 617 619 . . 619 629 + MwoI GCNNNNNNNGC 625 622 . . 619 623 + BlsI GCNGC 621 620 . . 619 623 + BisI GCNGC 620 621 . . 619 623 + TseI GCWGC 619 622 . . 622 627 + Cac8I GCNNGC 624 624 . . 619 623 - BbvI GCAGC 606 610 . . 626 629 + Hin6I GCGC 626 628 . . 626 629 + GlaI GCGC 627 627 . . 626 629 + HhaI GCGC 628 626 . . 628 632 - BceAI ACGGC 613 615 . . 644 649 + Hpy178III* TCNNGA 645 647 . . #--------------------------------------- # # # Acc65I: # [GGTACC] # 298 371 # # AccI: # [GTMKAC] # 153 158 358 # # AciI: # [CCGC] # 2 52 56 70 81 91 # 108 209 # # AgeI: # [ACCGGT] # 301 368 # # AluI: # [AGCT] # 196 473 # # AlwNI: # [CAGNNNCTG] # 102 116 142 309 # # ApaI: # [GGGCCC] # 120 549 # # AsuI*: # [GGNCC] # 1 112 123 139 139 155 # # AvaII: # [GGWCC] # 139 139 155 236 # # BbvI: # [GCAGC] # 33 63 125 448 # # BbvII*: # [GAAGAC] # 106 563 # # BceAI: # [ACGGC] # 34 56 258 321 # # BetI*: # [WCCGGW] # 301 368 # # BglII: # [AGATCT] # 213 456 # # BinI*: # [GGATC] # 255 414 # # BisI: # [GCNGC] # 3 10 47 49 115 445 # # BlsI: # [GCNGC] # 3 10 48 48 115 445 # # BmgT120I: # [GGNCC] # 1 112 122 139 140 155 # # BseBI: # [CCWGG] # 143 526 # # BseGI: # [GGATG] # 174 495 # # BseSI: # [GKGCMC] # 120 549 # # BseYI: # [CCCAGC] # 128 223 318 # # BsiYI*: # [CCNNNNNNNGG] # 4 87 167 184 227 # # BslFI: # [GGGAC] # 33 51 250 335 # # BsmI: # [GAATGC] # 48 107 514 # # Bsp120I: # [GGGCCC] # 124 545 # # BspMI: # [ACCTGC] # 323 346 # # BsrDI: # [GCAATG] # 55 614 # # BssKI: # [CCNGG] # 141 243 285 # # BstAPI: # [GCANNNNNTGC] # 309 360 # # BstKTI: # [GATC] # 47 216 406 # # Cac8I: # [GCNNGC] # 45 96 528 # # CauII*: # [CCSGG] # 283 386 # # Cfr10I: # [RCCGGY] # 301 368 # # CfrI: # [YGGCCR] # 55 57 557 # # Csp6I: # [GTAC] # 299 370 # # CviAII: # [CATG] # 74 78 154 363 # # CviJI: # [RGCY] # 9 13 16 17 39 50 # 53 57 57 64 69 225 # # CviRI*: # [TGCA] # 9 21 33 106 153 167 # 180 # # DdeI: # [CTNAG] # 16 64 77 164 348 # # DpnI: # [GATC] # 47 215 407 # # DraII: # [RGGNCCY] # 1 112 123 139 294 # # DsaI*: # [CCRYGG] # 50 66 79 220 254 # # EciI: # [GGCGGA] # 307 362 # # EcoP15I: # [CAGCAG] # 288 381 # # EcoRII: # [CCWGG] # 141 528 # # EcoT22I: # [ATGCAT] # 155 514 # # FatI: # [CATG] # 74 79 153 363 # # FauI: # [CCCGC] # 72 150 447 # # FnuDII*: # [CGCG] # 60 143 466 # # FokI: # [GGATG] # 161 508 # # GlaI: # [GCGC] # 18 42 69 540 # # GsaI: # [CCCAGC] # 124 223 322 # # HaeII: # [RGCGCY] # 18 109 542 # # HaeIII: # [GGCC] # 53 59 69 488 # # HgiCI*: # [GGYRCC] # 6 298 365 # # HgiJII*: # [GRGCYC] # 17 120 532 # # HhaI: # [GCGC] # 18 41 69 541 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 4 28 # 28 295 314 # # Hin4II*: # [CCTTC] # 37 172 222 238 # # Hin6I: # [GCGC] # 18 43 69 539 # # HindII: # [GTYRAC] # 159 510 # # HinfI: # [GANTC] # 45 210 414 # # HpaII: # [CCGG] # 84 283 302 # # Hpy166II: # [GTNNAC] # 34 119 159 357 # # Hpy178III*: # [TCNNGA] # 24 27 190 194 234 # # Hpy188I: # [TCNGA] # 74 260 335 # # KpnI: # [GGTACC] # 302 367 # # MaeI: # [CTAG] # 23 167 479 # # MaeIII: # [GTNAC] # 292 377 # # MboI: # [GATC] # 47 213 409 # # MboII: # [GAAGA] # 3 106 232 328 # # MfeI: # [CAATTG] # 272 397 # # MnlI: # [CCTC] # 53 57 57 78 82 96 # 246 # # MwoI: # [GCNNNNNNNGC] # 12 44 46 77 176 314 # # NcoI: # [CCATGG] # 79 590 # # NlaIII: # [CATG] # 74 75 157 363 # # NlaIV: # [GGNNCC] # 1 6 15 96 122 129 # 300 # # NmeAIII: # [GCCGAG] # 86 583 # # NspBII*: # [CMGCKG] # 50 93 526 # # NspI: # [RCATGY] # 157 512 # # PfoI: # [TCCNGGA] # 285 384 # # PpuMI: # [RGGWCCY] # 139 236 294 # # PstI: # [CTGCAG] # 304 365 # # RsaI: # [GTAC] # 300 369 # # SacII: # [CCGCGG] # 142 527 # # SalI: # [GTCGAC] # 157 512 # # SauI*: # [CCTNAGG] # 77 592 # # ScrFI: # [CCNGG] # 143 243 283 # # SduI: # [GDGCHC] # 17 120 532 # # SecI*: # [CCNNGG] # 12 50 66 67 220 254 # # SetI: # [ASST] # 12 27 28 30 33 78 # 87 107 118 149 # # SfaNI: # [GCATC] # 27 148 156 158 180 # # SfeI*: # [CTRYAG] # 308 361 # # SspI: # [AATATT] # 300 369 # # StyI: # [CCWWGG] # 79 590 # # TaqI: # [TCGA] # 48 110 511 # # TaqII: # [CACCCA] # 32 263 374 # # TauI: # [GCSGC] # 50 59 560 # # TfiI: # [GAWTC] # 45 210 414 # # TseI: # [GCWGC] # 46 50 125 448 # # Tsp4CI*: # [ACNGT] # 110 261 298 # # TspEI: # [AATT] # 6 89 183 391 # # XbaI: # [TCTAGA] # 189 480 # # XhoII: # [RGATCY] # 213 456 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 669 # Reported_sequences: 1 # Reported_hitcount: 251 #---------------------------------------