Restriction Fragments for YNLCsigma1



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The currently selected gene/sequence is :

YNLCsigma1 on chromosome XIV from coordinates 102700 to 102632.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.28520.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 16 27 + BdaI TGANNNNNNTCA 5 3 39 37 18 21 + SetI ASST 21 17 . . 17 20 - MnlI CCTC 10 9 . . 20 23 + Csp6I GTAC 20 22 . . 20 23 + RsaI GTAC 21 21 . . 22 25 + TaiI ACGT 25 21 . . 22 25 + SetI ASST 25 21 . . 22 25 + MaeII ACGT 22 24 . . 16 27 - BdaI TGANNNNNNTCA 39 37 5 3 19 29 - BaeI ACNNNNGTAYC 44 39 11 6 35 39 + TfiI GAWTC 35 38 . . 35 39 + HinfI GANTC 35 38 . . 35 45 + Hin4I GAYNNNNNVTC 26 21 58 53 35 45 - Hin4I GAYNNNNNVTC 58 53 26 21 49 54 + AclI AACGTT 50 52 . . 50 53 + TaiI ACGT 53 49 . . 50 53 + SetI ASST 53 49 . . 50 53 + MaeII ACGT 50 52 . . 64 69 + NdeI CATATG 65 67 . . #--------------------------------------- # # # AclI: # [AACGTT] # 19 50 # # BaeI: # [ACNNNNGTAYC] # 11 25 33 # # BdaI: # [TGANNNNNNTCA] # 0 0 5 30 34 # # Csp6I: # [GTAC] # 20 49 # # Hin4I: # [GAYNNNNNVTC] # 0 0 11 26 32 # # HinfI: # [GANTC] # 34 35 # # MaeII: # [ACGT] # 19 22 28 # # MnlI: # [CCTC] # 10 59 # # NdeI: # [CATATG] # 4 65 # # RsaI: # [GTAC] # 21 48 # # SetI: # [ASST] # 4 16 21 28 # # TaiI: # [ACGT] # 16 25 28 # # TfiI: # [GAWTC] # 34 35 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 69 # Reported_sequences: 1 # Reported_hitcount: 19 #---------------------------------------