Restriction Fragments for YNLCTy2-1



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The currently selected gene/sequence is :

YNLCTy2-1 on chromosome XIV from coordinates 567660 to 562364.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.26445.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 6 11 + HaeII RGCGCY 10 6 . . 7 10 + Hin6I GCGC 7 9 . . 7 10 + GlaI GCGC 8 8 . . 7 10 + HhaI GCGC 9 7 . . 21 25 + MaeIII GTNAC 20 25 . . 28 32 + DdeI CTNAG 28 31 . . 39 43 + BccI CCATC 47 48 . . 36 40 - BslFI GGGAC 21 25 . . 47 52 + Hpy178III* TCNNGA 48 50 . . 50 55 + XhoII RGATCY 50 54 . . 51 54 + DpnI GATC 52 52 . . 51 54 + BstKTI GATC 53 51 . . 51 54 + MboI GATC 50 54 . . 51 55 - BinI* GGATC 45 46 . . 53 57 - TspGWI ACGGA 43 41 . . 59 62 + CviJI RGCY 60 60 . . 60 70 + MwoI GCNNNNNNNGC 66 63 . . 60 64 - BceAI ACGGC 45 47 . . 68 71 + SetI ASST 71 67 . . 68 71 + CviJI RGCY 69 69 . . 68 71 + AluI AGCT 69 69 . . 75 78 + TspEI AATT 74 78 . . 80 85 + BetI* WCCGGW 80 84 . . 81 84 + HpaII CCGG 81 83 . . 84 89 + ApoI RAATTY 84 88 . . 85 88 + TspEI AATT 84 88 . . 96 100 + TfiI GAWTC 96 99 . . 96 100 + HinfI GANTC 96 99 . . 103 107 + DdeI CTNAG 103 106 . . 106 109 + SetI ASST 109 105 . . 109 112 + MseI TTAA 109 111 . . 111 114 + TspEI AATT 110 114 . . 123 127 - BsmAI GTCTC 117 121 . . 131 134 + SetI ASST 134 130 . . 132 136 + BssKI CCNGG 131 136 . . 132 136 + BseBI CCWGG 133 134 . . 132 137 + SecI* CCNNGG 132 136 . . 132 136 + ScrFI CCNGG 133 134 . . 132 136 + EcoRII CCWGG 131 136 . . 135 139 + BslFI GGGAC 149 153 . . 142 147 + PvuII CAGCTG 144 144 . . 142 147 + NspBII* CMGCKG 144 144 . . 143 146 + SetI ASST 146 142 . . 143 146 + CviJI RGCY 144 144 . . 143 146 + AluI AGCT 144 144 . . 149 154 + Hpy178III* TCNNGA 150 152 . . 158 162 + BccI CCATC 166 167 . . 159 168 + MslI CAYNNNNRTG 163 163 . . 165 168 + FatI CATG 164 168 . . 165 168 + NlaIII CATG 168 164 . . 165 168 + CviAII CATG 165 167 . . 168 172 + BsmAI GTCTC 173 177 . . 174 177 + MnlI CCTC 184 183 . . 172 177 - BseRI GAGGAG 163 161 . . 179 182 + SetI ASST 182 178 . . 179 184 + BspMI ACCTGC 188 192 . . 184 189 - Eco57MI CTGRAG 169 167 . . 189 192 + Csp6I GTAC 189 191 . . 189 192 + RsaI GTAC 190 190 . . 184 189 - Eco57I CTGAAG 169 167 . . 194 197 + SetI ASST 197 193 . . 195 198 + MnlI CCTC 205 204 . . 198 208 + BsiYI* CCNNNNNNNGG 204 201 . . 198 208 + PflMI CCANNNNNTGG 204 201 . . 209 213 + Tsp4CI* ACNGT 211 210 . . 212 215 + Csp6I GTAC 212 214 . . 212 215 + RsaI GTAC 213 213 . . 223 227 + BceAI ACGGC 239 241 . . 227 230 + FatI CATG 226 230 . . 227 230 + NlaIII CATG 230 226 . . 227 230 + CviAII CATG 227 229 . . 245 248 + SetI ASST 248 244 . . 245 248 + CviJI RGCY 246 246 . . 245 248 + AluI AGCT 246 246 . . 251 254 + CviJI RGCY 252 252 . . 251 254 + HaeIII GGCC 252 252 . . 253 256 + MnlI CCTC 263 262 . . 253 263 + BsiYI* CCNNNNNNNGG 259 256 . . 259 263 + BsrI ACTGG 264 262 . . 259 264 + BfiI ACTGGG 269 268 . . 262 267 + SduI GDGCHC 266 262 . . 262 267 + BseSI GKGCMC 266 262 . . 279 283 + BccI CCATC 287 288 . . 291 294 + TaiI ACGT 294 290 . . 291 294 + SetI ASST 294 290 . . 291 294 + MaeII ACGT 291 293 . . 291 296 + AflIII ACRYGT 291 295 . . 295 300 + Hpy166II GTNNAC 297 297 . . 290 295 - BtrI CACGTC 292 292 . . 309 312 + TaiI ACGT 312 308 . . 309 312 + SetI ASST 312 308 . . 309 312 + MaeII ACGT 309 311 . . 311 315 + Tsp45I GTSAC 310 315 . . 311 315 + MaeIII GTNAC 310 315 . . 313 319 + AarI CACCTGC 323 327 . . 314 317 + SetI ASST 317 313 . . 314 319 + BspMI ACCTGC 323 327 . . 312 316 - HphI GGTGA 304 303 . . 329 334 + BetI* WCCGGW 329 333 . . 330 333 + HpaII CCGG 330 332 . . 332 337 + NlaIV GGNNCC 334 334 . . 332 336 + BmgT120I GGNCC 333 334 . . 332 336 + AvaII GGWCC 332 335 . . 332 336 + AsuI* GGNCC 332 335 . . 344 348 - TspGWI ACGGA 334 332 . . 351 356 + DsaI* CCRYGG 351 355 . . 351 356 + SecI* CCNNGG 351 355 . . 352 363 + TstI CACNNNNNNTCC 343 338 375 370 353 357 + Tsp4CI* ACNGT 355 354 . . 367 373 + TspRI CASTGNN 373 364 . . 369 373 + DdeI CTNAG 369 372 . . 372 377 + TatI WGTACW 372 376 . . 372 377 + ScaI AGTACT 374 374 . . 373 376 + Csp6I GTAC 373 375 . . 369 373 - BspCNI CTCAG 361 359 . . 369 373 - BseMII CTCAG 360 358 . . 373 376 + RsaI GTAC 374 374 . . 379 382 + MnlI CCTC 389 388 . . 383 386 + SetI ASST 386 382 . . 381 385 - HphI GGTGA 373 372 . . 387 390 + DpnI GATC 388 388 . . 387 390 + BstKTI GATC 389 387 . . 387 390 + MboI GATC 386 390 . . 387 391 - BinI* GGATC 381 382 . . 393 398 + ClaI ATCGAT 394 396 . . 394 397 + TaqI TCGA 394 396 . . 398 403 + AhaIII* TTTAAA 400 400 . . 399 402 + MseI TTAA 399 401 . . 414 418 + Hpy188I TCNGA 416 415 . . 416 427 + BdaI TGANNNNNNTCA 405 403 439 437 422 425 + SetI ASST 425 421 . . 423 426 + MnlI CCTC 433 432 . . 424 429 + SmlI CTYRAG 424 428 . . 416 427 - BdaI TGANNNNNNTCA 439 437 405 403 428 431 + SetI ASST 431 427 . . 428 431 + CviJI RGCY 429 429 . . 428 431 + AluI AGCT 429 429 . . 424 429 - Bce83I* CTTGAG 409 407 . . 430 434 + DdeI CTNAG 430 433 . . 439 442 + SetI ASST 442 438 . . 451 454 + TaiI ACGT 454 450 . . 451 454 + SetI ASST 454 450 . . 451 454 + MaeII ACGT 451 453 . . 471 474 + MseI TTAA 471 473 . . 477 481 + Hpy188I TCNGA 479 478 . . 480 484 + MboII GAAGA 492 491 . . 496 499 + FatI CATG 495 499 . . 496 499 + NlaIII CATG 499 495 . . 496 499 + CviAII CATG 496 498 . . 502 505 + MseI TTAA 502 504 . . 504 509 + ApoI RAATTY 504 508 . . 505 508 + TspEI AATT 504 508 . . 514 518 + Hpy188I TCNGA 516 515 . . 524 528 + MboII GAAGA 536 535 . . 540 544 + HphI GGTGA 552 551 . . 541 545 + Tsp45I GTSAC 540 545 . . 541 545 + MaeIII GTNAC 540 545 . . 560 564 + BssKI CCNGG 559 564 . . 560 564 + BseBI CCWGG 561 562 . . 560 565 + SecI* CCNNGG 560 564 . . 560 564 + ScrFI CCNGG 561 562 . . 560 564 + EcoRII CCWGG 559 564 . . 564 568 + HphI GGTGA 576 575 . . 589 593 + TspDTI ATGAA 604 602 . . 591 595 + MboII GAAGA 603 602 . . 596 601 + NspI RCATGY 600 596 . . 597 600 + FatI CATG 596 600 . . 597 600 + NlaIII CATG 600 596 . . 597 600 + CviAII CATG 597 599 . . 615 618 + SetI ASST 618 614 . . 616 620 + Hin4II* CCTTC 626 625 . . 637 641 - TspDTI ATGAA 627 625 . . 652 655 + FatI CATG 651 655 . . 652 655 + NlaIII CATG 655 651 . . 652 655 + CviAII CATG 652 654 . . 664 669 + ApoI RAATTY 664 668 . . 665 668 + TspEI AATT 664 668 . . 674 677 + TspEI AATT 673 677 . . 675 680 + VspI ATTAAT 676 678 . . 676 679 + MseI TTAA 676 678 . . 678 681 + TspEI AATT 677 681 . . 689 693 - FokI GGATG 675 679 . . 689 693 - BseGI GGATG 688 686 . . 696 700 + Tsp4CI* ACNGT 698 697 . . 724 727 + CviRI* TGCA 725 725 . . 737 742 + Hpy178III* TCNNGA 738 740 . . 742 745 + TspEI AATT 741 745 . . 744 747 + MseI TTAA 744 746 . . 758 761 + SetI ASST 761 757 . . 758 761 + CviJI RGCY 759 759 . . 758 761 + AluI AGCT 759 759 . . 769 772 + SetI ASST 772 768 . . 771 776 + SmlI CTYRAG 771 775 . . 771 776 + Bce83I* CTTGAG 792 790 . . 776 779 + Csp6I GTAC 776 778 . . 776 779 + RsaI GTAC 777 777 . . 779 783 + Hpy99I CGWCG 783 778 . . 781 785 + TspGWI ACGGA 796 794 . . 786 791 + TatI WGTACW 786 790 . . 787 790 + Csp6I GTAC 787 789 . . 787 790 + RsaI GTAC 788 788 . . 793 798 - EcoP15I CAGCAG 765 767 . . 809 812 + TspEI AATT 808 812 . . 813 817 + Tsp4CI* ACNGT 815 814 . . 820 823 + Csp6I GTAC 820 822 . . 820 823 + RsaI GTAC 821 821 . . 824 827 + DpnI GATC 825 825 . . 824 827 + BstKTI GATC 826 824 . . 824 827 + MboI GATC 823 827 . . 830 834 + Hpy188I TCNGA 832 831 . . 835 838 + CviJI RGCY 836 836 . . 833 836 - MnlI CCTC 826 825 . . 872 875 + CviJI RGCY 873 873 . . 872 875 + HaeIII GGCC 873 873 . . 877 882 + Hpy166II GTNNAC 879 879 . . 877 882 + HindII GTYRAC 879 879 . . 889 892 + MseI TTAA 889 891 . . 893 896 + SetI ASST 896 892 . . 900 905 + BetI* WCCGGW 900 904 . . 901 904 + HpaII CCGG 901 903 . . 903 907 + HphI GGTGA 915 914 . . 904 908 + Tsp45I GTSAC 903 908 . . 904 908 + MaeIII GTNAC 903 908 . . 916 919 + SetI ASST 919 915 . . 919 924 + SnaBI TACGTA 921 921 . . 919 924 + BsaAI YACGTR 921 921 . . 920 923 + TaiI ACGT 923 919 . . 920 923 + SetI ASST 923 919 . . 920 923 + MaeII ACGT 920 922 . . 934 937 + Csp6I GTAC 934 936 . . 934 937 + RsaI GTAC 935 935 . . 947 950 + FatI CATG 946 950 . . 947 950 + NlaIII CATG 950 946 . . 947 950 + CviAII CATG 947 949 . . 948 952 + TspDTI ATGAA 963 961 . . 961 964 + TspEI AATT 960 964 . . 973 978 + ApoI RAATTY 973 977 . . 974 977 + TspEI AATT 973 977 . . 981 984 + MseI TTAA 981 983 . . 1003 1008 + Hpy178III* TCNNGA 1004 1006 . . 1003 1008 + BspHI TCATGA 1003 1007 . . 1004 1007 + FatI CATG 1003 1007 . . 1004 1007 + NlaIII CATG 1007 1003 . . 1004 1007 + CviAII CATG 1004 1006 . . 1005 1009 + TspDTI ATGAA 1020 1018 . . 1007 1011 + TfiI GAWTC 1007 1010 . . 1007 1011 + HinfI GANTC 1007 1010 . . 1019 1023 + Tsp4CI* ACNGT 1021 1020 . . 1022 1026 - BslFI GGGAC 1007 1011 . . 1090 1093 + SetI ASST 1093 1089 . . 1092 1096 + MaeIII GTNAC 1091 1096 . . 1104 1107 + TspEI AATT 1103 1107 . . 1109 1113 + Hpy188I TCNGA 1111 1110 . . 1137 1147 + MwoI GCNNNNNNNGC 1143 1140 . . 1137 1141 + BlsI GCNGC 1139 1138 . . 1137 1141 + BisI GCNGC 1138 1139 . . 1137 1141 + BbvI GCAGC 1149 1153 . . 1137 1141 + TseI GCWGC 1137 1140 . . 1145 1148 + SetI ASST 1148 1144 . . 1145 1148 + CviJI RGCY 1146 1146 . . 1145 1148 + AluI AGCT 1146 1146 . . 1152 1157 + SspI AATATT 1154 1154 . . 1165 1168 + MaeI CTAG 1165 1167 . . 1170 1175 + ApoI RAATTY 1170 1174 . . 1171 1174 + TspEI AATT 1170 1174 . . 1175 1180 + SmlI CTYRAG 1175 1179 . . 1175 1180 - Bce83I* CTTGAG 1160 1158 . . 1181 1185 + HphI GGTGA 1193 1192 . . 1182 1187 + Hpy166II GTNNAC 1184 1184 . . 1190 1195 + BclI TGATCA 1190 1194 . . 1191 1194 + DpnI GATC 1192 1192 . . 1191 1194 + BstKTI GATC 1193 1191 . . 1191 1194 + MboI GATC 1190 1194 . . 1194 1203 + MslI CAYNNNNRTG 1198 1198 . . 1197 1202 + VspI ATTAAT 1198 1200 . . 1198 1201 + MseI TTAA 1198 1200 . . 1201 1205 + TspDTI ATGAA 1216 1214 . . 1203 1207 + TfiI GAWTC 1203 1206 . . 1203 1207 + HinfI GANTC 1203 1206 . . 1209 1213 + Tsp4CI* ACNGT 1211 1210 . . 1214 1218 - TspDTI ATGAA 1204 1202 . . 1226 1231 + SmlI CTYRAG 1226 1230 . . 1226 1231 + AflII CTTAAG 1226 1230 . . 1227 1230 + MseI TTAA 1227 1229 . . 1231 1236 + BtgZI GCGATG 1246 1250 . . 1245 1249 + DdeI CTNAG 1245 1248 . . 1251 1255 + DdeI CTNAG 1251 1254 . . 1255 1258 + CviJI RGCY 1256 1256 . . 1255 1258 + HaeIII GGCC 1256 1256 . . 1256 1261 + Cac8I GCNNGC 1258 1258 . . 1270 1274 + TfiI GAWTC 1270 1273 . . 1270 1274 + HinfI GANTC 1270 1273 . . 1274 1278 + DdeI CTNAG 1274 1277 . . 1277 1280 + CviJI RGCY 1278 1278 . . 1288 1292 + Tsp4CI* ACNGT 1290 1289 . . 1291 1296 + Hpy166II GTNNAC 1293 1293 . . 1291 1296 + AccI GTMKAC 1292 1294 . . 1294 1298 + HinfI GANTC 1294 1297 . . 1297 1300 + TaqI TCGA 1297 1299 . . 1294 1298 - PleI GAGTC 1288 1289 . . 1294 1298 - MlyI GAGTC 1288 1288 . . 1311 1314 + SetI ASST 1314 1310 . . 1314 1319 + BclI TGATCA 1314 1318 . . 1315 1318 + DpnI GATC 1316 1316 . . 1315 1318 + BstKTI GATC 1317 1315 . . 1315 1318 + MboI GATC 1314 1318 . . 1319 1322 + SetI ASST 1322 1318 . . 1320 1324 + Hin4II* CCTTC 1330 1329 . . 1317 1321 - HphI GGTGA 1309 1308 . . 1330 1334 + TfiI GAWTC 1330 1333 . . 1330 1334 + HinfI GANTC 1330 1333 . . 1333 1338 + Hpy178III* TCNNGA 1334 1336 . . 1338 1341 + SetI ASST 1341 1337 . . 1338 1341 + CviJI RGCY 1339 1339 . . 1338 1341 + AluI AGCT 1339 1339 . . 1355 1359 + Hpy188I TCNGA 1357 1356 . . 1358 1361 + DpnI GATC 1359 1359 . . 1358 1361 + BstKTI GATC 1360 1358 . . 1358 1361 + MboI GATC 1357 1361 . . 1362 1365 + CviJI RGCY 1363 1363 . . 1378 1381 + FatI CATG 1377 1381 . . 1378 1381 + NlaIII CATG 1381 1377 . . 1378 1381 + CviAII CATG 1378 1380 . . 1380 1383 + CviRI* TGCA 1381 1381 . . 1385 1390 + TaqII CACCCA 1401 1399 . . 1390 1393 + TspEI AATT 1389 1393 . . 1393 1397 + Hpy188I TCNGA 1395 1394 . . 1409 1412 + TaqI TCGA 1409 1411 . . 1411 1415 - SfaNI GCATC 1401 1405 . . 1452 1456 - MboII GAAGA 1444 1443 . . 1467 1471 + Hpy188I TCNGA 1469 1468 . . 1472 1476 + BceAI ACGGC 1488 1490 . . 1474 1479 + NlaIV GGNNCC 1476 1476 . . 1474 1479 + HgiCI* GGYRCC 1474 1478 . . 1515 1518 + CviJI RGCY 1516 1516 . . 1522 1525 + DpnI GATC 1523 1523 . . 1522 1525 + BstKTI GATC 1524 1522 . . 1522 1525 + MboI GATC 1521 1525 . . 1528 1531 + MseI TTAA 1528 1530 . . 1541 1544 + SetI ASST 1544 1540 . . 1541 1544 + CviJI RGCY 1542 1542 . . 1541 1544 + AluI AGCT 1542 1542 . . 1542 1547 + Cac8I GCNNGC 1544 1544 . . 1545 1548 + CviJI RGCY 1546 1546 . . 1544 1549 - TsoI TARCCA 1534 1532 . . 1555 1560 + SspI AATATT 1557 1557 . . 1564 1569 + Cac8I GCNNGC 1566 1566 . . 1592 1595 + DpnI GATC 1593 1593 . . 1592 1595 + BstKTI GATC 1594 1592 . . 1592 1595 + MboI GATC 1591 1595 . . 1594 1598 + Hpy188I TCNGA 1596 1595 . . 1596 1600 + FokI GGATG 1609 1613 . . 1596 1600 + BseGI GGATG 1602 1600 . . 1601 1604 + Csp6I GTAC 1601 1603 . . 1597 1601 - BccI CCATC 1591 1592 . . 1601 1604 + RsaI GTAC 1602 1602 . . 1603 1607 + Tsp4CI* ACNGT 1605 1604 . . 1605 1610 + TatI WGTACW 1605 1609 . . 1605 1610 + ScaI AGTACT 1607 1607 . . 1606 1609 + Csp6I GTAC 1606 1608 . . 1606 1609 + RsaI GTAC 1607 1607 . . 1609 1612 + MaeI CTAG 1609 1611 . . 1611 1614 + SetI ASST 1614 1610 . . 1611 1614 + CviJI RGCY 1612 1612 . . 1611 1614 + AluI AGCT 1612 1612 . . 1627 1630 + FatI CATG 1626 1630 . . 1627 1630 + NlaIII CATG 1630 1626 . . 1627 1630 + CviAII CATG 1627 1629 . . 1631 1635 - BsmAI GTCTC 1625 1629 . . 1640 1644 + BsrI ACTGG 1645 1643 . . 1642 1647 - TsoI TARCCA 1632 1630 . . 1658 1661 + SetI ASST 1661 1657 . . 1662 1665 + TspEI AATT 1661 1665 . . 1666 1670 + Hin4II* CCTTC 1676 1675 . . 1682 1685 + SetI ASST 1685 1681 . . 1682 1685 + CviJI RGCY 1683 1683 . . 1682 1685 + AluI AGCT 1683 1683 . . 1697 1700 + TaiI ACGT 1700 1696 . . 1697 1700 + SetI ASST 1700 1696 . . 1697 1700 + MaeII ACGT 1697 1699 . . 1699 1704 + Hpy166II GTNNAC 1701 1701 . . 1699 1704 + HindII GTYRAC 1701 1701 . . 1734 1738 - TspGWI ACGGA 1724 1722 . . 1738 1741 + MseI TTAA 1738 1740 . . 1746 1749 + TaqI TCGA 1746 1748 . . 1748 1753 + BsmI GAATGC 1754 1752 . . 1758 1763 + NspI RCATGY 1762 1758 . . 1759 1762 + FatI CATG 1758 1762 . . 1759 1762 + NlaIII CATG 1762 1758 . . 1759 1762 + CviAII CATG 1759 1761 . . 1769 1773 + Hpy188I TCNGA 1771 1770 . . 1779 1783 + Hpy188I TCNGA 1781 1780 . . 1785 1789 + BsmAI GTCTC 1790 1794 . . 1789 1794 + SmlI CTYRAG 1789 1793 . . 1789 1794 + AflII CTTAAG 1789 1793 . . 1790 1793 + MseI TTAA 1790 1792 . . 1794 1798 + MboII GAAGA 1806 1805 . . 1797 1802 + BsmI GAATGC 1803 1801 . . 1800 1803 + CviRI* TGCA 1801 1801 . . 1804 1808 + MaeIII GTNAC 1803 1808 . . 1819 1823 + TfiI GAWTC 1819 1822 . . 1815 1819 - Hin4II* CCTTC 1809 1808 . . 1819 1823 + HinfI GANTC 1819 1822 . . 1822 1826 + Hpy188I TCNGA 1824 1823 . . 1843 1848 + Cac8I GCNNGC 1845 1845 . . 1843 1848 + BmtI GCTAGC 1847 1843 . . 1843 1848 + NheI GCTAGC 1843 1847 . . 1844 1847 + MaeI CTAG 1844 1846 . . 1850 1859 + MslI CAYNNNNRTG 1854 1854 . . 1860 1865 + Hpy178III* TCNNGA 1861 1863 . . 1865 1869 + Tsp4CI* ACNGT 1867 1866 . . 1891 1900 + MslI CAYNNNNRTG 1895 1895 . . 1896 1901 + BspLU11I* ACATGT 1896 1900 . . 1896 1901 + NspI RCATGY 1900 1896 . . 1896 1901 + AflIII ACRYGT 1896 1900 . . 1897 1900 + FatI CATG 1896 1900 . . 1897 1900 + NlaIII CATG 1900 1896 . . 1897 1900 + CviAII CATG 1897 1899 . . 1906 1910 + BinI* GGATC 1914 1915 . . 1907 1910 + DpnI GATC 1908 1908 . . 1907 1910 + BstKTI GATC 1909 1907 . . 1907 1910 + MboI GATC 1906 1910 . . 1909 1914 + Hpy178III* TCNNGA 1910 1912 . . 1914 1924 + BaeI ACNNNNGTAYC 1903 1898 1936 1931 1920 1923 + Csp6I GTAC 1920 1922 . . 1920 1923 + RsaI GTAC 1921 1921 . . 1927 1931 + TfiI GAWTC 1927 1930 . . 1927 1931 + HinfI GANTC 1927 1930 . . 1931 1936 + NdeI CATATG 1932 1934 . . 1935 1946 + BdaI TGANNNNNNTCA 1924 1922 1958 1956 1937 1940 + CviJI RGCY 1938 1938 . . 1946 1951 + TatI WGTACW 1946 1950 . . 1935 1946 - BdaI TGANNNNNNTCA 1958 1956 1924 1922 1946 1951 + ScaI AGTACT 1948 1948 . . 1947 1950 + Csp6I GTAC 1947 1949 . . 1947 1950 + RsaI GTAC 1948 1948 . . 1952 1955 + CviRI* TGCA 1953 1953 . . 1969 1973 + BmgT120I GGNCC 1970 1971 . . 1969 1973 + AvaII GGWCC 1969 1972 . . 1969 1973 + AsuI* GGNCC 1969 1972 . . 1974 1979 + TatI WGTACW 1974 1978 . . 1974 1979 + Bsp1407I TGTACA 1974 1978 . . 1975 1978 + Csp6I GTAC 1975 1977 . . 1975 1978 + RsaI GTAC 1976 1976 . . 1992 2002 + FalI AAGNNNNNCTT 1983 1978 2015 2010 1994 1999 + Hpy166II GTNNAC 1996 1996 . . 1994 1999 + SduI GDGCHC 1998 1994 . . 1994 1999 + BseSI GKGCMC 1998 1994 . . 1994 1999 + ApaLI GTGCAC 1994 1998 . . 1994 1999 + HgiAI* GWGCWC 1998 1994 . . 1995 1998 + CviRI* TGCA 1996 1996 . . 1998 2001 + SetI ASST 2001 1997 . . 1999 2003 + Hin4II* CCTTC 2009 2008 . . 1992 2002 - FalI AAGNNNNNCTT 2015 2010 1983 1978 2016 2021 + Hpy166II GTNNAC 2018 2018 . . 2036 2040 + TfiI GAWTC 2036 2039 . . 2036 2040 + HinfI GANTC 2036 2039 . . 2047 2052 + Hpy166II GTNNAC 2049 2049 . . 2044 2049 - TaqII CACCCA 2034 2032 . . 2049 2052 + Csp6I GTAC 2049 2051 . . 2049 2052 + RsaI GTAC 2050 2050 . . 2061 2066 + McrI* CGRYCG 2064 2062 . . 2063 2067 + Tsp4CI* ACNGT 2065 2064 . . 2065 2069 + Hpy99I CGWCG 2069 2064 . . 2067 2072 + Hpy178III* TCNNGA 2068 2070 . . 2071 2075 + MboII GAAGA 2083 2082 . . 2074 2078 + TfiI GAWTC 2074 2077 . . 2074 2078 + HinfI GANTC 2074 2077 . . 2082 2085 + MnlI CCTC 2092 2091 . . 2097 2102 + ClaI ATCGAT 2098 2100 . . 2098 2101 + TaqI TCGA 2098 2100 . . 2114 2117 + MseI TTAA 2114 2116 . . 2118 2127 + XmnI GAANNNNTTC 2122 2122 . . 2123 2126 + TspEI AATT 2122 2126 . . 2131 2136 + Cac8I GCNNGC 2133 2133 . . 2134 2137 + FnuDII* CGCG 2135 2135 . . 2140 2143 + MaeI CTAG 2140 2142 . . 2147 2152 + Hpy178III* TCNNGA 2148 2150 . . 2148 2162 + XcmI CCANNNNNNNNNTGG 2155 2154 . . 2154 2158 + BinI* GGATC 2162 2163 . . 2155 2165 + Hin4I GAYNNNNNVTC 2146 2141 2178 2173 2155 2158 + DpnI GATC 2156 2156 . . 2155 2158 + BstKTI GATC 2157 2155 . . 2155 2158 + MboI GATC 2154 2158 . . 2151 2155 - BccI CCATC 2145 2146 . . 2161 2164 + CviJI RGCY 2162 2162 . . 2161 2166 + NlaIV GGNNCC 2163 2163 . . 2164 2168 + Hpy188I TCNGA 2166 2165 . . 2168 2173 + TatI WGTACW 2168 2172 . . 2169 2174 + Hpy166II GTNNAC 2171 2171 . . 2169 2172 + Csp6I GTAC 2169 2171 . . 2169 2172 + RsaI GTAC 2170 2170 . . 2183 2188 + Ksp632I* CTCTTC 2189 2192 . . 2184 2188 - MboII GAAGA 2176 2175 . . 2186 2190 - TspDTI ATGAA 2176 2174 . . 2208 2211 + SetI ASST 2211 2207 . . 2207 2210 - MnlI CCTC 2200 2199 . . 2217 2220 + CviRI* TGCA 2218 2218 . . 2218 2223 + NspI RCATGY 2222 2218 . . 2218 2223 + Cac8I GCNNGC 2220 2220 . . 2218 2223 + SphI GCATGC 2222 2218 . . 2219 2222 + FatI CATG 2218 2222 . . 2219 2222 + NlaIII CATG 2222 2218 . . 2219 2222 + CviAII CATG 2219 2221 . . 2231 2236 + DsaI* CCRYGG 2231 2235 . . 2231 2236 + SecI* CCNNGG 2231 2235 . . 2233 2237 + BceAI ACGGC 2249 2251 . . 2239 2243 + TfiI GAWTC 2239 2242 . . 2239 2243 + HinfI GANTC 2239 2242 . . 2242 2247 + XbaI TCTAGA 2242 2246 . . 2242 2247 + Hpy178III* TCNNGA 2243 2245 . . 2243 2246 + MaeI CTAG 2243 2245 . . 2246 2251 + SduI GDGCHC 2250 2246 . . 2246 2251 + HgiAI* GWGCWC 2250 2246 . . 2249 2257 + DraIII CACNNNGTG 2254 2251 . . 2252 2256 + Tsp4CI* ACNGT 2254 2253 . . 2269 2272 + MseI TTAA 2269 2271 . . 2276 2279 + Csp6I GTAC 2276 2278 . . 2276 2279 + RsaI GTAC 2277 2277 . . 2284 2287 + MseI TTAA 2284 2286 . . 2300 2306 + TspRI CASTGNN 2306 2297 . . 2300 2305 - BtsI GCAGTG 2299 2297 . . 2308 2317 + MslI CAYNNNNRTG 2312 2312 . . 2306 2310 - TspDTI ATGAA 2296 2294 . . 2311 2314 + CviRI* TGCA 2312 2312 . . 2312 2317 + BtsI GCAGTG 2319 2317 . . 2313 2319 + TspRI CASTGNN 2319 2310 . . 2308 2313 - BsrDI GCAATG 2307 2305 . . 2318 2323 + Hpy166II GTNNAC 2320 2320 . . 2318 2323 + AccI GTMKAC 2319 2321 . . 2323 2337 + XcmI CCANNNNNNNNNTGG 2330 2329 . . 2340 2344 + DdeI CTNAG 2340 2343 . . 2340 2344 + BspCNI CTCAG 2353 2351 . . 2340 2344 + BseMII CTCAG 2354 2352 . . 2342 2347 + EcoP15I CAGCAG 2372 2374 . . 2348 2351 + TaqI TCGA 2348 2350 . . 2350 2355 + ApoI RAATTY 2350 2354 . . 2351 2354 + TspEI AATT 2350 2354 . . 2366 2370 + Hpy188I TCNGA 2368 2367 . . 2370 2375 + ApoI RAATTY 2370 2374 . . 2371 2374 + TspEI AATT 2370 2374 . . 2373 2377 - TspDTI ATGAA 2363 2361 . . 2380 2384 + BsmAI GTCTC 2385 2389 . . 2383 2387 - HphI GGTGA 2375 2374 . . 2402 2405 + AciI CCGC 2402 2404 . . 2412 2417 + NspI RCATGY 2416 2412 . . 2413 2416 + FatI CATG 2412 2416 . . 2413 2416 + NlaIII CATG 2416 2412 . . 2413 2416 + CviAII CATG 2413 2415 . . 2415 2418 + CviRI* TGCA 2416 2416 . . 2416 2426 + MwoI GCNNNNNNNGC 2422 2419 . . 2418 2421 + SetI ASST 2421 2417 . . 2416 2421 - BspMI ACCTGC 2407 2411 . . 2424 2427 + SetI ASST 2427 2423 . . 2424 2427 + CviJI RGCY 2425 2425 . . 2424 2427 + AluI AGCT 2425 2425 . . 2430 2434 + BsrI ACTGG 2435 2433 . . 2451 2454 + SetI ASST 2454 2450 . . 2459 2464 + Hpy166II GTNNAC 2461 2461 . . 2459 2464 + HindII GTYRAC 2461 2461 . . 2456 2461 - TaqII GACCGA 2446 2444 . . 2463 2468 + Cfr10I RCCGGY 2463 2467 . . 2463 2468 + BetI* WCCGGW 2463 2467 . . 2463 2468 + AgeI ACCGGT 2463 2467 . . 2464 2467 + HpaII CCGG 2464 2466 . . 2473 2478 + Hpy166II GTNNAC 2475 2475 . . 2473 2478 + HpaI GTTAAC 2475 2475 . . 2473 2478 + HindII GTYRAC 2475 2475 . . 2474 2477 + MseI TTAA 2474 2476 . . 2487 2492 + Hpy178III* TCNNGA 2488 2490 . . 2491 2495 + HinfI GANTC 2491 2494 . . 2494 2497 + TaqI TCGA 2494 2496 . . 2491 2495 - PleI GAGTC 2485 2486 . . 2491 2495 - MlyI GAGTC 2485 2485 . . 2503 2512 + MslI CAYNNNNRTG 2507 2507 . . 2506 2509 + MnlI CCTC 2516 2515 . . 2503 2507 - FokI GGATG 2489 2493 . . 2503 2507 - BseGI GGATG 2502 2500 . . 2507 2512 - BsiI* CACGAG 2507 2511 . . 2518 2522 + BssKI CCNGG 2517 2522 . . 2518 2522 + BseBI CCWGG 2519 2520 . . 2513 2518 - BsmI GAATGC 2513 2511 . . 2518 2522 + ScrFI CCNGG 2519 2520 . . 2518 2522 + EcoRII CCWGG 2517 2522 . . 2520 2523 + SetI ASST 2523 2519 . . 2522 2526 + MaeIII GTNAC 2521 2526 . . 2533 2537 - FokI GGATG 2519 2523 . . 2533 2537 - BseGI GGATG 2532 2530 . . 2538 2542 + Tsp45I GTSAC 2537 2542 . . 2538 2542 + MaeIII GTNAC 2537 2542 . . 2535 2539 - TspGWI ACGGA 2525 2523 . . 2539 2544 + Hpy178III* TCNNGA 2540 2542 . . 2554 2557 + CviJI RGCY 2555 2555 . . 2553 2558 - TsoI TARCCA 2543 2541 . . 2568 2572 - MboII GAAGA 2560 2559 . . 2572 2576 + BccI CCATC 2580 2581 . . 2578 2581 + MseI TTAA 2578 2580 . . 2587 2591 + Tsp4CI* ACNGT 2589 2588 . . 2602 2605 + TspEI AATT 2601 2605 . . 2606 2609 + TaiI ACGT 2609 2605 . . 2606 2609 + SetI ASST 2609 2605 . . 2606 2609 + MaeII ACGT 2606 2608 . . 2633 2636 + TspEI AATT 2632 2636 . . 2637 2641 + BmgT120I GGNCC 2638 2639 . . 2637 2641 + AvaII GGWCC 2637 2640 . . 2637 2641 + AsuI* GGNCC 2637 2640 . . 2640 2644 - BsrI ACTGG 2640 2638 . . 2645 2648 + TaqI TCGA 2645 2647 . . 2668 2677 + BsaBI GATNNNNATC 2672 2672 . . 2674 2677 + DpnI GATC 2675 2675 . . 2674 2677 + BstKTI GATC 2676 2674 . . 2674 2683 + BsaBI GATNNNNATC 2678 2678 . . 2674 2677 + MboI GATC 2673 2677 . . 2686 2689 + MseI TTAA 2686 2688 . . 2691 2694 + CviJI RGCY 2692 2692 . . 2697 2702 + TsoI TARCCA 2713 2711 . . 2720 2724 + TspDTI ATGAA 2735 2733 . . 2725 2729 + TspGWI ACGGA 2740 2738 . . 2735 2740 + NdeI CATATG 2736 2738 . . 2739 2744 + BclI TGATCA 2739 2743 . . 2740 2743 + DpnI GATC 2741 2741 . . 2740 2743 + BstKTI GATC 2742 2740 . . 2740 2743 + MboI GATC 2739 2743 . . 2750 2758 + AlwNI CAGNNNCTG 2755 2752 . . 2752 2756 + TfiI GAWTC 2752 2755 . . 2752 2756 + HinfI GANTC 2752 2755 . . 2755 2759 + Hpy188I TCNGA 2757 2756 . . 2757 2762 + BclI TGATCA 2757 2761 . . 2758 2761 + DpnI GATC 2759 2759 . . 2758 2761 + BstKTI GATC 2760 2758 . . 2758 2761 + MboI GATC 2757 2761 . . 2760 2765 + Hpy178III* TCNNGA 2761 2763 . . 2760 2765 + BspHI TCATGA 2760 2764 . . 2761 2764 + FatI CATG 2760 2764 . . 2761 2764 + NlaIII CATG 2764 2760 . . 2761 2764 + CviAII CATG 2761 2763 . . 2764 2774 + Hin4I GAYNNNNNVTC 2755 2750 2787 2782 2773 2777 + Hpy188I TCNGA 2775 2774 . . 2764 2774 - Hin4I GAYNNNNNVTC 2787 2782 2755 2750 2791 2795 + Hpy188I TCNGA 2793 2792 . . 2794 2797 + DpnI GATC 2795 2795 . . 2794 2797 + BstKTI GATC 2796 2794 . . 2794 2797 + MboI GATC 2793 2797 . . 2797 2800 + MnlI CCTC 2807 2806 . . 2794 2798 - BinI* GGATC 2788 2789 . . 2800 2803 + MaeI CTAG 2800 2802 . . 2803 2808 + Hpy166II GTNNAC 2805 2805 . . 2805 2816 + PpiI GAACNNNNNCTC 2797 2792 2829 2824 2805 2814 + XmnI GAANNNNTTC 2809 2809 . . 2809 2819 + Hin4I GAYNNNNNVTC 2800 2795 2832 2827 2809 2819 + Eam1105I GACNNNNNGTC 2814 2813 . . 2809 2819 - Hin4I GAYNNNNNVTC 2832 2827 2800 2795 2817 2821 - BslFI GGGAC 2802 2806 . . 2840 2843 + TspEI AATT 2839 2843 . . 2850 2855 + NlaIV GGNNCC 2852 2852 . . 2854 2858 - BsrI ACTGG 2854 2852 . . 2866 2869 + Csp6I GTAC 2866 2868 . . 2866 2869 + RsaI GTAC 2867 2867 . . 2867 2872 + BsaAI YACGTR 2869 2869 . . 2868 2871 + TaiI ACGT 2871 2867 . . 2868 2871 + SetI ASST 2871 2867 . . 2868 2871 + MaeII ACGT 2868 2870 . . 2870 2875 + Hpy166II GTNNAC 2872 2872 . . 2870 2875 + SduI GDGCHC 2874 2870 . . 2870 2875 + BseSI GKGCMC 2874 2870 . . 2870 2875 + ApaLI GTGCAC 2870 2874 . . 2870 2875 + HgiAI* GWGCWC 2874 2870 . . 2871 2874 + CviRI* TGCA 2872 2872 . . 2887 2891 - SfaNI GCATC 2877 2881 . . 2899 2903 + Hpy188I TCNGA 2901 2900 . . 2908 2913 + SspI AATATT 2910 2910 . . 2913 2917 - MboII GAAGA 2905 2904 . . 2917 2921 + BccI CCATC 2925 2926 . . 2930 2933 + DpnI GATC 2931 2931 . . 2930 2933 + BstKTI GATC 2932 2930 . . 2930 2933 + MboI GATC 2929 2933 . . 2936 2941 + Hpy166II GTNNAC 2938 2938 . . 2936 2939 + Csp6I GTAC 2936 2938 . . 2936 2939 + RsaI GTAC 2937 2937 . . 2950 2955 + VspI ATTAAT 2951 2953 . . 2951 2954 + MseI TTAA 2951 2953 . . 2963 2966 + Csp6I GTAC 2963 2965 . . 2963 2966 + RsaI GTAC 2964 2964 . . 2977 2982 + KpnI GGTACC 2981 2977 . . 2977 2982 + NlaIV GGNNCC 2979 2979 . . 2977 2982 + HgiCI* GGYRCC 2977 2981 . . 2977 2982 + Acc65I GGTACC 2977 2981 . . 2978 2981 + Csp6I GTAC 2978 2980 . . 2978 2981 + RsaI GTAC 2979 2979 . . 2980 2984 + Tsp4CI* ACNGT 2982 2981 . . 2986 2990 + TfiI GAWTC 2986 2989 . . 2986 2990 + HinfI GANTC 2986 2989 . . 2989 2993 + Hpy188I TCNGA 2991 2990 . . 3003 3006 + SetI ASST 3006 3002 . . 3005 3008 + MaeI CTAG 3005 3007 . . 3001 3005 - HphI GGTGA 2993 2992 . . 3008 3017 + PshAI GACNNNNGTC 3012 3012 . . 3015 3020 + Hpy166II GTNNAC 3017 3017 . . 3015 3020 + AccI GTMKAC 3016 3018 . . 3019 3022 + SetI ASST 3022 3018 . . 3020 3024 + Hin4II* CCTTC 3030 3029 . . 3024 3030 + TspRI CASTGNN 3030 3021 . . 3027 3030 + CviRI* TGCA 3028 3028 . . 3024 3029 - BtsI GCAGTG 3023 3021 . . 3026 3031 - BsgI GTGCAG 3011 3009 . . 3036 3050 + XcmI CCANNNNNNNNNTGG 3043 3042 . . 3044 3055 + DrdI GACNNNNNNGTC 3050 3048 . . 3045 3048 + SetI ASST 3048 3044 . . 3045 3051 + SexAI ACCWGGT 3045 3050 . . 3046 3050 + BssKI CCNGG 3045 3050 . . 3046 3050 + BseBI CCWGG 3047 3048 . . 3046 3050 + ScrFI CCNGG 3047 3048 . . 3046 3050 + EcoRII CCWGG 3045 3050 . . 3053 3057 - BslFI GGGAC 3038 3042 . . 3070 3080 + Hin4I GAYNNNNNVTC 3061 3056 3093 3088 3076 3079 + SetI ASST 3079 3075 . . 3077 3080 + MnlI CCTC 3087 3086 . . 3092 3095 + MseI TTAA 3092 3094 . . 3094 3097 + TspEI AATT 3093 3097 . . 3121 3126 + AflIII ACRYGT 3121 3125 . . 3123 3126 + TaiI ACGT 3126 3122 . . 3123 3126 + SetI ASST 3126 3122 . . 3123 3126 + MaeII ACGT 3123 3125 . . 3128 3133 - TaqII CACCCA 3118 3116 . . 3134 3137 + Csp6I GTAC 3134 3136 . . 3134 3137 + RsaI GTAC 3135 3135 . . 3136 3140 + TspGWI ACGGA 3151 3149 . . 3141 3146 + NlaIV GGNNCC 3143 3143 . . 3139 3142 - MnlI CCTC 3132 3131 . . 3197 3200 + Csp6I GTAC 3197 3199 . . 3197 3200 + RsaI GTAC 3198 3198 . . 3203 3206 + MnlI CCTC 3213 3212 . . 3214 3224 + Hin4I GAYNNNNNVTC 3205 3200 3237 3232 3214 3224 + Eam1105I GACNNNNNGTC 3219 3218 . . 3215 3219 + Tsp4CI* ACNGT 3217 3216 . . 3223 3228 + MmeI TCCRAC 3248 3246 . . 3214 3224 - Hin4I GAYNNNNNVTC 3237 3232 3205 3200 3227 3231 + Tsp4CI* ACNGT 3229 3228 . . 3228 3234 + TspRI CASTGNN 3234 3225 . . 3232 3236 + TfiI GAWTC 3232 3235 . . 3232 3236 + HinfI GANTC 3232 3235 . . 3245 3249 + BccI CCATC 3253 3254 . . 3267 3270 + CviJI RGCY 3268 3268 . . 3287 3290 + MnlI CCTC 3297 3296 . . 3295 3304 + BsaBI GATNNNNATC 3299 3299 . . 3298 3302 + TspGWI ACGGA 3313 3311 . . 3300 3305 + XhoII RGATCY 3300 3304 . . 3300 3305 + NlaIV GGNNCC 3302 3302 . . 3300 3305 + BamHI GGATCC 3300 3304 . . 3300 3304 + BinI* GGATC 3308 3309 . . 3301 3304 + DpnI GATC 3302 3302 . . 3301 3304 + BstKTI GATC 3303 3301 . . 3301 3304 + MboI GATC 3300 3304 . . 3304 3307 + AciI CCGC 3304 3306 . . 3301 3305 - BinI* GGATC 3295 3296 . . 3303 3308 - EciI GGCGGA 3293 3291 . . 3314 3319 + ClaI ATCGAT 3315 3317 . . 3315 3318 + TaqI TCGA 3315 3317 . . 3317 3320 + DpnI GATC 3318 3318 . . 3317 3320 + BstKTI GATC 3319 3317 . . 3317 3320 + MboI GATC 3316 3320 . . 3322 3326 + Hpy188I TCNGA 3324 3323 . . 3319 3323 - MboII GAAGA 3311 3310 . . 3328 3331 + FatI CATG 3327 3331 . . 3328 3331 + NlaIII CATG 3331 3327 . . 3328 3331 + CviAII CATG 3328 3330 . . 3333 3337 + HinfI GANTC 3333 3336 . . 3333 3337 - PleI GAGTC 3327 3328 . . 3333 3337 - MlyI GAGTC 3327 3327 . . 3352 3362 + Hin4I GAYNNNNNVTC 3343 3338 3375 3370 3359 3362 + MnlI CCTC 3369 3368 . . 3370 3374 + MaeIII GTNAC 3369 3374 . . 3373 3377 + BceAI ACGGC 3389 3391 . . 3375 3378 + CviJI RGCY 3376 3376 . . 3379 3383 + TfiI GAWTC 3379 3382 . . 3379 3383 + HinfI GANTC 3379 3382 . . 3387 3392 + Hpy178III* TCNNGA 3388 3390 . . 3395 3398 + SetI ASST 3398 3394 . . 3396 3399 + MnlI CCTC 3406 3405 . . 3405 3410 + Hpy178III* TCNNGA 3406 3408 . . 3412 3415 + MseI TTAA 3412 3414 . . 3417 3421 + BccI CCATC 3425 3426 . . 3430 3434 + TspGWI ACGGA 3445 3443 . . 3439 3443 + Hpy188I TCNGA 3441 3440 . . 3477 3481 + Hpy188I TCNGA 3479 3478 . . 3484 3487 + TspEI AATT 3483 3487 . . 3488 3492 - BsrI ACTGG 3488 3486 . . 3494 3499 - TaqII CACCCA 3484 3482 . . 3504 3508 + FokI GGATG 3517 3521 . . 3504 3508 + BseGI GGATG 3510 3508 . . 3508 3512 + TfiI GAWTC 3508 3511 . . 3508 3512 + HinfI GANTC 3508 3511 . . 3519 3523 + Hpy188I TCNGA 3521 3520 . . 3545 3548 + DpnI GATC 3546 3546 . . 3545 3548 + BstKTI GATC 3547 3545 . . 3545 3548 + MboI GATC 3544 3548 . . 3553 3557 + MboII GAAGA 3565 3564 . . 3560 3564 + TspDTI ATGAA 3575 3573 . . 3570 3573 + TspEI AATT 3569 3573 . . 3575 3578 + SetI ASST 3578 3574 . . 3574 3577 - MnlI CCTC 3567 3566 . . 3577 3582 + BciVI GTATCC 3588 3587 . . 3580 3585 + Hpy178III* TCNNGA 3581 3583 . . 3581 3586 + BmeT110I CYCGRG 3582 3584 . . 3581 3586 + AvaI CYCGRG 3581 3585 . . 3584 3588 - BsmAI GTCTC 3578 3582 . . 3590 3593 + FatI CATG 3589 3593 . . 3590 3593 + NlaIII CATG 3593 3589 . . 3590 3593 + CviAII CATG 3590 3592 . . 3615 3620 + Hpy178III* TCNNGA 3616 3618 . . 3618 3623 + NlaIV GGNNCC 3620 3620 . . 3629 3632 + DpnI GATC 3630 3630 . . 3629 3632 + BstKTI GATC 3631 3629 . . 3629 3632 + MboI GATC 3628 3632 . . 3631 3634 + TaqI TCGA 3631 3633 . . 3633 3637 + MboII GAAGA 3645 3644 . . 3645 3650 + ApoI RAATTY 3645 3649 . . 3646 3649 + TspEI AATT 3645 3649 . . 3649 3652 + MseI TTAA 3649 3651 . . 3651 3654 + TspEI AATT 3650 3654 . . 3654 3657 + CviRI* TGCA 3655 3655 . . 3655 3659 + BlsI GCNGC 3657 3656 . . 3655 3659 + BisI GCNGC 3656 3657 . . 3655 3659 + BbvI GCAGC 3667 3671 . . 3655 3659 + TseI GCWGC 3655 3658 . . 3671 3682 + BdaI TGANNNNNNTCA 3660 3658 3694 3692 3675 3680 + ClaI ATCGAT 3676 3678 . . 3676 3679 + TaqI TCGA 3676 3678 . . 3678 3681 + DpnI GATC 3679 3679 . . 3678 3681 + BstKTI GATC 3680 3678 . . 3678 3681 + MboI GATC 3677 3681 . . 3671 3682 - BdaI TGANNNNNNTCA 3694 3692 3660 3658 3685 3689 - BsrI ACTGG 3685 3683 . . 3689 3694 + AsuII TTCGAA 3690 3692 . . 3690 3693 + TaqI TCGA 3690 3692 . . 3697 3700 + SetI ASST 3700 3696 . . 3699 3704 + SmlI CTYRAG 3699 3703 . . 3699 3704 + AflII CTTAAG 3699 3703 . . 3700 3703 + MseI TTAA 3700 3702 . . 3710 3714 + TspDTI ATGAA 3725 3723 . . 3717 3720 + TspEI AATT 3716 3720 . . 3723 3728 + PsiI TTATAA 3725 3725 . . 3759 3770 + BdaI TGANNNNNNTCA 3748 3746 3782 3780 3761 3766 + HindIII AAGCTT 3761 3765 . . 3762 3765 + SetI ASST 3765 3761 . . 3762 3765 + CviJI RGCY 3763 3763 . . 3762 3765 + AluI AGCT 3763 3763 . . 3759 3770 - BdaI TGANNNNNNTCA 3782 3780 3748 3746 3777 3780 + TspEI AATT 3776 3780 . . 3780 3785 + TsoI TARCCA 3796 3794 . . 3781 3784 + CviJI RGCY 3782 3782 . . 3796 3800 + TspDTI ATGAA 3811 3809 . . 3807 3812 - BciVI GTATCC 3801 3800 . . 3818 3823 + SspI AATATT 3820 3820 . . 3840 3845 + XhoII RGATCY 3840 3844 . . 3841 3844 + DpnI GATC 3842 3842 . . 3841 3844 + BstKTI GATC 3843 3841 . . 3841 3844 + MboI GATC 3840 3844 . . 3841 3845 - BinI* GGATC 3835 3836 . . 3875 3878 + MseI TTAA 3875 3877 . . 3885 3888 + TaiI ACGT 3888 3884 . . 3885 3888 + SetI ASST 3888 3884 . . 3885 3888 + MaeII ACGT 3885 3887 . . 3893 3898 + Hpy166II GTNNAC 3895 3895 . . 3893 3896 + Csp6I GTAC 3893 3895 . . 3889 3893 - BccI CCATC 3883 3884 . . 3893 3896 + RsaI GTAC 3894 3894 . . 3903 3906 + SetI ASST 3906 3902 . . 3903 3906 + CviJI RGCY 3904 3904 . . 3903 3906 + AluI AGCT 3904 3904 . . 3905 3908 + MaeI CTAG 3905 3907 . . 3915 3918 + CviRI* TGCA 3916 3916 . . 3920 3923 - MnlI CCTC 3913 3912 . . 3944 3949 + NdeI CATATG 3945 3947 . . 3949 3953 + TfiI GAWTC 3949 3952 . . 3949 3953 + HinfI GANTC 3949 3952 . . 3952 3956 + Hpy188I TCNGA 3954 3953 . . 3955 3965 + Hin4I GAYNNNNNVTC 3946 3941 3978 3973 3959 3962 + CviRI* TGCA 3960 3960 . . 3955 3965 - Hin4I GAYNNNNNVTC 3978 3973 3946 3941 3970 3974 + Tsp4CI* ACNGT 3972 3971 . . 3973 3976 + Csp6I GTAC 3973 3975 . . 3973 3976 + RsaI GTAC 3974 3974 . . 3976 3985 + MslI CAYNNNNRTG 3980 3980 . . 3984 3987 + CviRI* TGCA 3985 3985 . . 3986 3992 + TspRI CASTGNN 3992 3983 . . 3993 3998 + ZraI GACGTC 3995 3995 . . 3993 3998 + AcyI GRCGYC 3994 3996 . . 3993 3998 + AatII GACGTC 3997 3993 . . 3993 4004 + DrdI GACNNNNNNGTC 3999 3997 . . 3994 3997 + TaiI ACGT 3997 3993 . . 3994 3997 + SetI ASST 3997 3993 . . 3994 3997 + MaeII ACGT 3994 3996 . . 3996 4000 + Tsp45I GTSAC 3995 4000 . . 3996 4000 + MaeIII GTNAC 3995 4000 . . 3998 4004 + TspRI CASTGNN 4004 3995 . . 3999 4003 + Tsp4CI* ACNGT 4001 4000 . . 4036 4041 + PvuII CAGCTG 4038 4038 . . 4036 4041 + NspBII* CMGCKG 4038 4038 . . 4037 4040 + SetI ASST 4040 4036 . . 4037 4040 + CviJI RGCY 4038 4038 . . 4037 4040 + AluI AGCT 4038 4038 . . 4042 4052 + Hin4I GAYNNNNNVTC 4033 4028 4065 4060 4049 4052 + MnlI CCTC 4059 4058 . . 4069 4074 + EcoRV GATATC 4071 4071 . . 4078 4082 + MboII GAAGA 4090 4089 . . 4082 4085 + TspEI AATT 4081 4085 . . 4095 4098 + SetI ASST 4098 4094 . . 4096 4099 + MnlI CCTC 4106 4105 . . 4102 4112 + BsiYI* CCNNNNNNNGG 4108 4105 . . 4110 4113 + SetI ASST 4113 4109 . . 4124 4127 + TspEI AATT 4123 4127 . . 4131 4134 + TaiI ACGT 4134 4130 . . 4131 4134 + SetI ASST 4134 4130 . . 4131 4134 + MaeII ACGT 4131 4133 . . 4170 4173 + CviRI* TGCA 4171 4171 . . 4175 4179 + BsrI ACTGG 4180 4178 . . 4181 4185 + TspDTI ATGAA 4196 4194 . . 4190 4193 + MseI TTAA 4190 4192 . . 4201 4204 + MseI TTAA 4201 4203 . . 4207 4210 + TspEI AATT 4206 4210 . . 4217 4222 + NspI RCATGY 4221 4217 . . 4218 4221 + FatI CATG 4217 4221 . . 4218 4221 + NlaIII CATG 4221 4217 . . 4218 4221 + CviAII CATG 4218 4220 . . 4220 4223 + CviRI* TGCA 4221 4221 . . 4231 4234 + FnuDII* CGCG 4232 4232 . . 4221 4232 - BcgI CGANNNNNNTGC 4244 4242 4210 4208 4234 4238 + FokI GGATG 4247 4251 . . 4234 4238 + BseGI GGATG 4240 4238 . . 4232 4235 - AciI CCGC 4232 4234 . . 4235 4239 - BccI CCATC 4229 4230 . . 4241 4244 + FatI CATG 4240 4244 . . 4241 4244 + NlaIII CATG 4244 4240 . . 4241 4244 + CviAII CATG 4241 4243 . . 4249 4254 + AhaIII* TTTAAA 4251 4251 . . 4250 4253 + MseI TTAA 4250 4252 . . 4264 4268 + MaeIII GTNAC 4263 4268 . . 4269 4272 + TspEI AATT 4268 4272 . . 4292 4303 + BdaI TGANNNNNNTCA 4281 4279 4315 4313 4292 4303 - BdaI TGANNNNNNTCA 4315 4313 4281 4279 4312 4315 + MseI TTAA 4312 4314 . . 4317 4320 + CviRI* TGCA 4318 4318 . . 4342 4347 + SmlI CTYRAG 4342 4346 . . 4343 4348 + Hpy178III* TCNNGA 4344 4346 . . 4342 4347 - Bce83I* CTTGAG 4327 4325 . . 4365 4375 + Hin4I GAYNNNNNVTC 4356 4351 4388 4383 4365 4375 - Hin4I GAYNNNNNVTC 4388 4383 4356 4351 4378 4382 + HphI GGTGA 4390 4389 . . 4376 4381 - TaqII CACCCA 4366 4364 . . 4401 4404 + Csp6I GTAC 4401 4403 . . 4401 4404 + RsaI GTAC 4402 4402 . . 4415 4425 + Hin4I GAYNNNNNVTC 4406 4401 4438 4433 4422 4432 + Hin4I GAYNNNNNVTC 4413 4408 4445 4440 4422 4425 + DpnI GATC 4423 4423 . . 4422 4425 + BstKTI GATC 4424 4422 . . 4422 4425 + MboI GATC 4421 4425 . . 4415 4425 - Hin4I GAYNNNNNVTC 4438 4433 4406 4401 4422 4432 - Hin4I GAYNNNNNVTC 4445 4440 4413 4408 4442 4447 + TatI WGTACW 4442 4446 . . 4443 4446 + Csp6I GTAC 4443 4445 . . 4443 4446 + RsaI GTAC 4444 4444 . . 4446 4449 + FatI CATG 4445 4449 . . 4446 4449 + NlaIII CATG 4449 4445 . . 4446 4449 + CviAII CATG 4446 4448 . . 4447 4451 + TspDTI ATGAA 4462 4460 . . 4451 4454 + TspEI AATT 4450 4454 . . 4455 4458 + SetI ASST 4458 4454 . . 4481 4484 + TspEI AATT 4480 4484 . . 4496 4499 + TaiI ACGT 4499 4495 . . 4496 4499 + SetI ASST 4499 4495 . . 4496 4499 + MaeII ACGT 4496 4498 . . 4498 4501 + Csp6I GTAC 4498 4500 . . 4498 4501 + RsaI GTAC 4499 4499 . . 4500 4503 + SetI ASST 4503 4499 . . 4525 4529 + DdeI CTNAG 4525 4528 . . 4529 4534 + SacI GAGCTC 4533 4529 . . 4529 4534 + Ecl136II GAGCTC 4531 4531 . . 4529 4534 + SduI GDGCHC 4533 4529 . . 4529 4534 + HgiJII* GRGCYC 4533 4529 . . 4529 4534 + HgiAI* GWGCWC 4533 4529 . . 4530 4533 + SetI ASST 4533 4529 . . 4530 4533 + CviJI RGCY 4531 4531 . . 4530 4533 + AluI AGCT 4531 4531 . . 4534 4538 + BssKI CCNGG 4533 4538 . . 4534 4538 + BseBI CCWGG 4535 4536 . . 4534 4538 + ScrFI CCNGG 4535 4536 . . 4534 4538 + EcoRII CCWGG 4533 4538 . . 4536 4539 + SetI ASST 4539 4535 . . 4532 4537 - Eco57MI CTGRAG 4517 4515 . . 4532 4537 - GsuI CTGGAG 4517 4515 . . 4538 4543 + Hpy166II GTNNAC 4540 4540 . . 4538 4543 + HindII GTYRAC 4540 4540 . . 4542 4548 + SexAI ACCWGGT 4542 4547 . . 4543 4547 + BssKI CCNGG 4542 4547 . . 4543 4547 + BseBI CCWGG 4544 4545 . . 4543 4547 + ScrFI CCNGG 4544 4545 . . 4543 4547 + EcoRII CCWGG 4542 4547 . . 4545 4548 + SetI ASST 4548 4544 . . 4560 4564 + BssKI CCNGG 4559 4564 . . 4560 4564 + BseBI CCWGG 4561 4562 . . 4560 4564 + ScrFI CCNGG 4561 4562 . . 4560 4564 + EcoRII CCWGG 4559 4564 . . 4563 4567 + FokI GGATG 4576 4580 . . 4563 4567 + BseGI GGATG 4569 4567 . . 4565 4569 + TspDTI ATGAA 4580 4578 . . 4570 4573 + MaeI CTAG 4570 4572 . . 4580 4584 + TspDTI ATGAA 4595 4593 . . 4582 4586 + MboII GAAGA 4594 4593 . . 4586 4590 + TspDTI ATGAA 4601 4599 . . 4602 4607 + TatI WGTACW 4602 4606 . . 4603 4606 + Csp6I GTAC 4603 4605 . . 4603 4606 + RsaI GTAC 4604 4604 . . 4606 4609 + FatI CATG 4605 4609 . . 4606 4609 + NlaIII CATG 4609 4605 . . 4606 4609 + CviAII CATG 4606 4608 . . 4607 4611 + TspDTI ATGAA 4622 4620 . . 4613 4616 + CviRI* TGCA 4614 4614 . . 4630 4633 + MaeI CTAG 4630 4632 . . 4632 4635 + SetI ASST 4635 4631 . . 4632 4635 + CviJI RGCY 4633 4633 . . 4632 4635 + AluI AGCT 4633 4633 . . 4637 4642 + NdeI CATATG 4638 4640 . . 4635 4639 - TspDTI ATGAA 4625 4623 . . 4642 4647 - MmeI TCCRAC 4623 4621 . . 4651 4656 + ApoI RAATTY 4651 4655 . . 4652 4655 + TspEI AATT 4651 4655 . . 4709 4712 + MnlI CCTC 4719 4718 . . 4712 4715 + MaeI CTAG 4712 4714 . . 4728 4731 + FatI CATG 4727 4731 . . 4728 4731 + NlaIII CATG 4731 4727 . . 4728 4731 + CviAII CATG 4728 4730 . . 4733 4738 + NdeI CATATG 4734 4736 . . 4741 4744 + MseI TTAA 4741 4743 . . 4747 4758 + CspCI CAANNNNNGTGG 4735 4733 4770 4768 4748 4751 + TspEI AATT 4747 4751 . . 4752 4755 + FatI CATG 4751 4755 . . 4752 4755 + NlaIII CATG 4755 4751 . . 4752 4755 + CviAII CATG 4752 4754 . . 4750 4754 - TspDTI ATGAA 4740 4738 . . 4757 4761 + BslFI GGGAC 4771 4775 . . 4763 4766 + MaeI CTAG 4763 4765 . . 4775 4778 + TspEI AATT 4774 4778 . . 4776 4781 + VspI ATTAAT 4777 4779 . . 4777 4780 + MseI TTAA 4777 4779 . . 4797 4800 + SetI ASST 4800 4796 . . 4805 4808 + CviJI RGCY 4806 4806 . . 4818 4823 + SpeI ACTAGT 4818 4822 . . 4819 4822 + MaeI CTAG 4819 4821 . . 4830 4840 + FalI AAGNNNNNCTT 4821 4816 4853 4848 4832 4837 + BtgZI GCGATG 4847 4851 . . 4834 4838 - SfaNI GCATC 4824 4828 . . 4830 4840 - FalI AAGNNNNNCTT 4853 4848 4821 4816 4841 4846 + NdeI CATATG 4842 4844 . . 4839 4843 - TspDTI ATGAA 4829 4827 . . 4846 4852 + BstEII GGTNACC 4846 4851 . . 4847 4851 + MaeIII GTNAC 4846 4851 . . 4848 4853 + TsoI TARCCA 4864 4862 . . 4866 4870 + Tsp45I GTSAC 4865 4870 . . 4866 4870 + MaeIII GTNAC 4865 4870 . . 4872 4875 + TspEI AATT 4871 4875 . . 4877 4881 + MaeIII GTNAC 4876 4881 . . 4895 4899 + TspGWI ACGGA 4910 4908 . . 4910 4913 - MnlI CCTC 4903 4902 . . 4917 4922 + SalI GTCGAC 4917 4921 . . 4917 4922 + Hpy166II GTNNAC 4919 4919 . . 4917 4922 + HindII GTYRAC 4919 4919 . . 4917 4922 + AccI GTMKAC 4918 4920 . . 4918 4921 + TaqI TCGA 4918 4920 . . 4926 4929 + CviJI RGCY 4927 4927 . . 4932 4937 + Hpy166II GTNNAC 4934 4934 . . 4932 4937 + HpaI GTTAAC 4934 4934 . . 4932 4937 + HindII GTYRAC 4934 4934 . . 4933 4936 + MseI TTAA 4933 4935 . . 4936 4941 + NspI RCATGY 4940 4936 . . 4937 4940 + FatI CATG 4936 4940 . . 4937 4940 + NlaIII CATG 4940 4936 . . 4937 4940 + CviAII CATG 4937 4939 . . 4939 4942 + CviRI* TGCA 4940 4940 . . 4949 4954 + SfeI* CTRYAG 4949 4953 . . 4954 4966 + BarI GAAGNNNNNNTAC 4946 4941 4978 4973 4974 4980 + TspRI CASTGNN 4980 4971 . . 4978 4987 + XmnI GAANNNNTTC 4982 4982 . . 4980 4983 + SetI ASST 4983 4979 . . 4980 4983 + CviJI RGCY 4981 4981 . . 4980 4983 + AluI AGCT 4981 4981 . . 4987 4990 + AciI CCGC 4987 4989 . . 4994 5005 + BdaI TGANNNNNNTCA 4983 4981 5017 5015 5000 5003 + SetI ASST 5003 4999 . . 5001 5004 + MnlI CCTC 5011 5010 . . 5002 5006 + DdeI CTNAG 5002 5005 . . 5002 5006 + BspCNI CTCAG 5015 5013 . . 5002 5006 + BseMII CTCAG 5016 5014 . . 4994 5005 - BdaI TGANNNNNNTCA 5017 5015 4983 4981 5006 5010 + Tsp45I GTSAC 5005 5010 . . 5006 5010 + MaeIII GTNAC 5005 5010 . . 5008 5016 + DraIII CACNNNGTG 5013 5010 . . 5009 5012 + SetI ASST 5012 5008 . . 5007 5011 - HphI GGTGA 4999 4998 . . 5015 5018 + CviRI* TGCA 5016 5016 . . 5024 5027 + MseI TTAA 5024 5026 . . 5037 5040 + TspEI AATT 5036 5040 . . 5042 5045 + MseI TTAA 5042 5044 . . 5045 5055 + FalI AAGNNNNNCTT 5036 5031 5068 5063 5047 5050 + CviJI RGCY 5048 5048 . . 5045 5055 - FalI AAGNNNNNCTT 5068 5063 5036 5031 5059 5069 + Hin4I GAYNNNNNVTC 5050 5045 5082 5077 5066 5076 + Hin4I GAYNNNNNVTC 5057 5052 5089 5084 5066 5069 + DpnI GATC 5067 5067 . . 5066 5069 + BstKTI GATC 5068 5066 . . 5066 5069 + MboI GATC 5065 5069 . . 5059 5069 - Hin4I GAYNNNNNVTC 5082 5077 5050 5045 5073 5076 + DpnI GATC 5074 5074 . . 5073 5076 + BstKTI GATC 5075 5073 . . 5073 5076 + MboI GATC 5072 5076 . . 5066 5076 - Hin4I GAYNNNNNVTC 5089 5084 5057 5052 5082 5085 + TspEI AATT 5081 5085 . . 5084 5087 + MseI TTAA 5084 5086 . . 5088 5093 + Hpy166II GTNNAC 5090 5090 . . 5088 5093 + AccI GTMKAC 5089 5091 . . 5096 5100 + TspDTI ATGAA 5111 5109 . . 5098 5102 + MboII GAAGA 5110 5109 . . 5098 5103 - Ksp632I* CTCTTC 5093 5096 . . 5104 5109 + ApoI RAATTY 5104 5108 . . 5105 5108 + TspEI AATT 5104 5108 . . 5134 5139 + BsrDI GCAATG 5141 5139 . . 5143 5147 + DdeI CTNAG 5143 5146 . . 5139 5143 - BsmAI GTCTC 5133 5137 . . 5149 5159 + Hin4I GAYNNNNNVTC 5140 5135 5172 5167 5150 5154 + TspDTI ATGAA 5165 5163 . . 5149 5159 - Hin4I GAYNNNNNVTC 5172 5167 5140 5135 5160 5163 + SetI ASST 5163 5159 . . 5167 5170 + TspEI AATT 5166 5170 . . 5173 5178 + SnaBI TACGTA 5175 5175 . . 5173 5178 + BsaAI YACGTR 5175 5175 . . 5174 5177 + TaiI ACGT 5177 5173 . . 5174 5177 + SetI ASST 5177 5173 . . 5174 5177 + MaeII ACGT 5174 5176 . . 5176 5181 + Hpy166II GTNNAC 5178 5178 . . 5176 5181 + BssNAI GTATAC 5178 5178 . . 5176 5181 + AccI GTMKAC 5177 5179 . . 5186 5189 + TaqI TCGA 5186 5188 . . 5186 5191 + Hpy178III* TCNNGA 5187 5189 . . 5188 5192 - BsmAI GTCTC 5182 5186 . . 5188 5193 - Eco31I GGTCTC 5182 5186 . . 5196 5200 + MboII GAAGA 5208 5207 . . 5202 5207 - BsrDI GCAATG 5201 5199 . . 5217 5227 + Hin4I GAYNNNNNVTC 5208 5203 5240 5235 5223 5226 + SetI ASST 5226 5222 . . 5224 5227 + MnlI CCTC 5234 5233 . . 5225 5230 + Ksp632I* CTCTTC 5231 5234 . . 5229 5233 + Hpy188I TCNGA 5231 5230 . . 5226 5230 - MboII GAAGA 5218 5217 . . 5242 5247 + AhaIII* TTTAAA 5244 5244 . . 5243 5246 + MseI TTAA 5243 5245 . . 5251 5254 + MseI TTAA 5251 5253 . . 5265 5269 + TfiI GAWTC 5265 5268 . . 5265 5269 + HinfI GANTC 5265 5268 . . 5267 5271 - TspDTI ATGAA 5257 5255 . . 5273 5278 + XhoII RGATCY 5273 5277 . . 5273 5278 + BglII AGATCT 5273 5277 . . 5274 5277 + DpnI GATC 5275 5275 . . 5274 5277 + BstKTI GATC 5276 5274 . . 5274 5277 + MboI GATC 5273 5277 . . 5288 5293 - TaqII CACCCA 5278 5276 . . #--------------------------------------- # # # AarI: # [CACCTGC] # 323 4974 # # AatII: # [GACGTC] # 1300 3997 # # Acc65I: # [GGTACC] # 2320 2977 # # AccI: # [GTMKAC] # 88 120 171 697 1027 1292 # 1902 # # AciI: # [CCGC] # 310 755 902 928 2402 # # AcyI: # [GRCGYC] # 1303 3994 # # AflII: # [CTTAAG] # 563 1226 1598 1910 # # AflIII: # [ACRYGT] # 291 1225 1605 2176 # # AgeI: # [ACCGGT] # 2463 2834 # # AhaIII*: # [TTTAAA] # 53 400 993 3851 # # AluI: # [AGCT] # 69 70 71 75 102 102 # 134 141 183 193 203 316 # 330 348 387 493 742 1338 # # AlwNI: # [CAGNNNCTG] # 2542 2755 # # ApaLI: # [GTGCAC] # 876 1994 2427 # # ApoI: # [RAATTY] # 20 84 160 193 197 309 # 420 453 1006 1180 1275 # # AsuI*: # [GGNCC] # 332 668 1637 2660 # # AsuII: # [TTCGAA] # 1607 3690 # # AvaI: # [CYCGRG] # 1716 3581 # # AvaII: # [GGWCC] # 332 668 1637 2660 # # BaeI: # [ACNNNNGTAYC] # 33 1903 3361 # # BamHI: # [GGATCC] # 1997 3300 # # BarI: # [GAAGNNNNNNTAC] # 32 319 4946 # # BbvI: # [GCAGC] # 1149 1630 2518 # # BccI: # [CCATC] # 47 119 121 172 328 345 # 346 435 458 554 1068 1304 # # Bce83I*: # [CTTGAG] # 368 383 409 970 3167 # # BceAI: # [ACGGC] # 45 194 761 1140 1249 1908 # # BcgI: # [CGANNNNNNTGC] # 34 1053 4210 # # BciVI: # [GTATCC] # 213 1496 3588 # # BclI: # [TGATCA] # 18 124 1190 1425 2540 # # BdaI: # [TGANNNNNNTCA] # 0 0 0 0 0 0 # 0 0 0 0 0 0 # 34 34 34 34 34 34 # 54 280 405 499 668 1485 # 1702 # # BetI*: # [WCCGGW] # 80 249 571 1563 2834 # # BfiI: # [ACTGGG] # 269 5028 # # BglII: # [AGATCT] # 24 5273 # # BinI*: # [GGATC] # 13 45 248 336 507 527 # 626 1462 1533 # # BisI: # [GCNGC] # 1138 1641 2518 # # BlsI: # [GCNGC] # 1139 1640 2518 # # BmeT110I: # [CYCGRG] # 1715 3582 # # BmgT120I: # [GGNCC] # 333 668 1637 2659 # # BmtI: # [GCTAGC] # 1847 3450 # # BsaAI: # [YACGTR] # 122 921 1948 2306 # # BsaBI: # [GATNNNNATC] # 6 621 1998 2672 # # BseBI: # [CCWGG] # 9 17 133 428 528 736 # 1488 1958 # # BseGI: # [GGATG] # 30 329 688 728 730 900 # 914 978 # # BseMII: # [CTCAG] # 281 360 1994 2662 # # BseRI: # [GAGGAG] # 163 5134 # # BseSI: # [GKGCMC] # 266 876 1732 2423 # # BsgI: # [GTGCAG] # 2286 3011 # # BsiI*: # [CACGAG] # 2507 2790 # # BsiYI*: # [CCNNNNNNNGG] # 55 204 1189 3849 # # BslFI: # [GGGAC] # 21 128 236 526 858 1733 # 1795 # # BsmAI: # [GTCTC] # 49 56 115 117 165 595 # 1193 1452 1555 # # BsmI: # [GAATGC] # 49 710 1754 2784 # # Bsp1407I: # [TGTACA] # 1974 3323 # # BspCNI: # [CTCAG] # 282 361 1992 2662 # # BspHI: # [TCATGA] # 1003 1757 2537 # # BspLU11I*: # [ACATGT] # 1896 3401 # # BspMI: # [ACCTGC] # 135 188 2084 2890 # # BsrDI: # [GCAATG] # 60 96 2307 2834 # # BsrI: # [ACTGG] # 197 205 214 264 495 634 # 790 1117 1381 # # BssKI: # [CCNGG] # 9 17 131 428 528 738 # 1488 1958 # # BssNAI: # [GTATAC] # 119 5178 # # BstEII: # [GGTNACC] # 451 4846 # # BstKTI: # [GATC] # 7 16 18 21 36 43 # 49 53 66 70 84 124 # 136 163 164 201 228 248 # 315 336 367 371 437 519 # 581 644 # # BtgZI: # [GCGATG] # 450 1246 3601 # # BtrI: # [CACGTC] # 292 5005 # # BtsI: # [GCAGTG] # 20 704 2274 2299 # # Cac8I: # [GCNNGC] # 22 87 279 286 288 1258 # 3077 # # Cfr10I: # [RCCGGY] # 2463 2834 # # ClaI: # [ATCGAT] # 361 394 1217 1621 1704 # # Csp6I: # [GTAC] # 5 11 15 23 27 27 # 28 33 42 55 63 70 # 74 80 105 107 114 120 # 156 161 189 314 403 428 # 590 667 694 696 # # CspCI: # [CAANNNNNGTGG] # 35 527 4735 # # CviAII: # [CATG] # 23 24 55 57 62 101 # 122 132 138 160 165 185 # 194 205 249 262 269 295 # 322 348 360 374 567 628 # # CviJI: # [RGCY] # 4 6 9 19 22 24 # 26 37 54 60 61 66 # 67 71 75 77 102 102 # 108 110 121 122 130 134 # 137 153 173 177 224 249 # 255 263 273 330 387 493 # 576 # # CviRI*: # [TGCA] # 25 43 44 50 76 94 # 97 104 152 156 186 222 # 261 281 296 326 420 456 # 627 656 725 # # DdeI: # [CTNAG] # 6 23 28 61 75 141 # 154 266 477 815 1066 2185 # # DpnI: # [GATC] # 7 16 18 22 36 43 # 49 52 66 70 84 124 # 136 163 164 201 228 248 # 315 336 367 371 437 519 # 581 644 # # DraIII: # [CACNNNGTG] # 284 2254 2759 # # DrdI: # [GACNNNNNNGTC] # 949 1298 3050 # # DsaI*: # [CCRYGG] # 351 1880 3066 # # Eam1105I: # [GACNNNNNGTC] # 405 2078 2814 # # EciI: # [GGCGGA] # 2004 3293 # # Ecl136II: # [GAGCTC] # 766 4531 # # Eco31I: # [GGTCTC] # 115 5182 # # Eco57I: # [CTGAAG] # 169 5128 # # Eco57MI: # [CTGRAG] # 169 780 4348 # # EcoP15I: # [CAGCAG] # 765 1607 2925 # # EcoRII: # [CCWGG] # 9 17 131 428 528 738 # 1488 1958 # # EcoRV: # [GATATC] # 1226 4071 # # FalI: # [AAGNNNNNCTT] # 0 0 0 0 0 0 # 32 32 32 183 229 1983 # 2806 # # FatI: # [CATG] # 23 24 55 57 62 101 # 122 132 138 160 164 185 # 194 205 249 262 269 295 # 322 348 361 374 567 628 # # FnuDII*: # [CGCG] # 1065 2097 2135 # # FokI: # [GGATG] # 30 329 675 721 730 880 # 934 998 # # GlaI: # [GCGC] # 8 5289 # # GsuI: # [CTGGAG] # 780 4517 # # HaeII: # [RGCGCY] # 10 5287 # # HaeIII: # [GGCC] # 252 383 621 4041 # # HgiAI*: # [GWGCWC] # 252 624 764 1659 1998 # # HgiCI*: # [GGYRCC] # 1474 1503 2320 # # HgiJII*: # [GRGCYC] # 764 4533 # # HhaI: # [GCGC] # 9 5288 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 0 0 # 0 0 0 0 0 0 # 0 0 0 0 0 0 # 0 0 7 7 7 7 # 13 18 25 25 32 32 # 32 32 32 32 32 32 # 32 32 32 36 51 55 # 57 106 112 229 291 571 # 577 605 2146 # # Hin4II*: # [CCTTC] # 133 200 346 626 704 1021 # 2267 # # Hin6I: # [GCGC] # 7 5290 # # HindII: # [GTYRAC] # 14 15 363 379 760 822 # 879 2065 # # HindIII: # [AAGCTT] # 1536 3761 # # HinfI: # [GANTC] # 24 32 36 38 46 67 # 96 101 108 109 129 165 # 196 234 246 252 261 441 # 489 911 1316 # # HpaI: # [GTTAAC] # 363 2459 2475 # # HpaII: # [CCGG] # 81 249 571 1563 2833 # # HphI: # [GGTGA] # 24 69 116 179 278 298 # 304 339 609 618 1066 1397 # # Hpy166II: # [GTNNAC] # 14 15 22 31 66 67 # 79 88 109 119 122 141 # 149 156 295 297 305 330 # 379 408 582 645 878 # # Hpy178III*: # [TCNNGA] # 18 35 48 49 52 80 # 95 102 110 158 175 221 # 245 266 330 527 588 627 # 728 843 # # Hpy188I: # [TCNGA] # 10 18 18 37 38 38 # 42 43 63 66 74 90 # 108 117 127 175 202 246 # 279 316 333 342 389 416 # 433 1277 # # Hpy99I: # [CGWCG] # 783 1286 3228 # # KpnI: # [GGTACC] # 2316 2981 # # Ksp632I*: # [CTCTTC] # 66 138 2189 2904 # # MaeI: # [CTAG] # 51 56 60 82 103 205 # 235 296 444 478 557 665 # 900 1165 # # MaeII: # [ACGT] # 18 109 123 137 142 255 # 262 291 365 469 678 762 # 777 909 # # MaeIII: # [GTNAC] # 11 16 19 20 129 188 # 230 268 290 292 363 583 # 626 712 718 832 # # MboI: # [GATC] # 7 16 18 24 36 43 # 49 50 66 70 84 124 # 136 163 164 201 228 248 # 315 336 367 371 437 519 # 581 644 # # MboII: # [GAAGA] # 10 44 67 79 80 93 # 98 254 277 345 362 384 # 406 445 492 504 516 841 # # McrI*: # [CGRYCG] # 2064 3233 # # MlyI: # [GAGTC] # 842 1197 1288 1970 # # MmeI: # [TCCRAC] # 674 1375 3248 # # MnlI: # [CCTC] # 21 37 44 45 47 58 # 63 72 81 84 108 108 # 126 146 161 184 184 223 # 280 291 316 346 393 613 # 1266 # # MseI: # [TTAA] # 8 11 15 18 29 31 # 36 42 46 49 51 52 # 62 68 72 91 92 104 # 108 109 141 145 155 156 # 159 174 175 190 210 217 # 237 265 290 301 315 320 # 324 429 # # MslI: # [CAYNNNNRTG] # 41 163 195 417 656 1035 # 1317 1473 # # MwoI: # [GCNNNNNNNGC] # 66 1077 1279 2875 # # NdeI: # [CATATG] # 96 108 455 693 804 1209 # 1932 # # NheI: # [GCTAGC] # 1843 3454 # # NlaIII: # [CATG] # 23 24 55 57 62 101 # 122 132 138 160 168 185 # 194 205 249 262 269 295 # 322 348 357 374 567 628 # # NlaIV: # [GGNNCC] # 127 159 164 318 334 687 # 689 1142 1677 # # NspBII*: # [CMGCKG] # 144 1259 3894 # # NspI: # [RCATGY] # 138 194 322 357 600 719 # 1162 1805 # # PflMI: # [CCANNNNNTGG] # 204 5093 # # PleI: # [GAGTC] # 842 1197 1288 1970 # # PpiI: # [GAACNNNNNCTC] # 32 2468 2797 # # PshAI: # [GACNNNNGTC] # 2285 3012 # # PsiI: # [TTATAA] # 1572 3725 # # PvuII: # [CAGCTG] # 144 1259 3894 # # RsaI: # [GTAC] # 5 11 15 23 27 27 # 28 33 42 55 63 70 # 74 80 105 107 114 120 # 156 161 190 314 403 428 # 590 667 693 696 # # SacI: # [GAGCTC] # 764 4533 # # SalI: # [GTCGAC] # 380 4917 # # ScaI: # [AGTACT] # 341 374 1233 3349 # # ScrFI: # [CCNGG] # 9 17 133 428 528 736 # 1488 1958 # # SduI: # [GDGCHC] # 252 266 624 764 1659 1732 # # SecI*: # [CCNNGG] # 132 209 219 1671 3066 # # SetI: # [ASST] # 4 4 5 6 6 6 # 8 9 9 11 11 12 # 12 14 15 15 15 16 # 18 18 19 20 21 23 # 24 25 26 27 30 31 # 36 38 39 41 43 46 # 47 47 49 51 55 58 # 65 69 69 70 71 71 # 86 87 91 122 123 124 # 135 143 151 164 165 166 # 170 180 183 203 210 210 # 262 272 301 324 # # SexAI: # [ACCWGGT] # 755 1497 3045 # # SfaNI: # [GCATC] # 473 1401 1476 1947 # # SfeI*: # [CTRYAG] # 348 4949 # # SmlI: # [CTYRAG] # 51 347 404 424 563 643 # 955 1910 # # SnaBI: # [TACGTA] # 122 921 4254 # # SpeI: # [ACTAGT] # 479 4818 # # SphI: # [GCATGC] # 2222 3075 # # SspI: # [AATATT] # 403 910 1154 1353 1477 # # TaiI: # [ACGT] # 18 109 120 137 142 255 # 262 294 365 469 678 762 # 777 909 # # TaqI: # [TCGA] # 14 45 111 112 146 151 # 250 268 316 337 352 394 # 670 903 1228 # # TaqII: # [CACCCA] # 19 366 412 633 672 882 # 912 1401 # # TatI: # [WGTACW] # 28 160 194 341 372 414 # 695 819 2274 # # TfiI: # [GAWTC] # 32 38 60 67 96 108 # 109 129 147 165 196 234 # 246 441 489 513 911 1316 # # TseI: # [GCWGC] # 1137 1642 2518 # # TsoI: # [TARCCA] # 98 170 433 911 1068 1083 # 1534 # # Tsp45I: # [GTSAC] # 140 230 292 310 363 870 # 1458 1634 # # Tsp4CI*: # [ACNGT] # 12 29 79 117 144 189 # 190 198 206 211 235 262 # 315 335 343 393 743 1296 # # TspDTI: # [ATGAA] # 3 6 12 15 21 23 # 40 54 57 67 86 89 # 92 115 118 120 150 184 # 266 282 336 372 385 604 # 840 960 # # TspEI: # [AATT] # 4 5 9 10 13 20 # 23 26 27 30 31 42 # 45 60 62 62 64 66 # 67 67 74 76 83 86 # 96 97 130 131 152 160 # 165 171 182 207 219 228 # 231 254 272 305 390 394 # 461 # # TspGWI: # [ACGGA] # 43 132 162 215 291 387 # 411 462 801 928 1465 # # TspRI: # [CASTGNN] # 12 13 204 317 373 711 # 758 976 1933 # # TstI: # [CACNNNNNNTCC] # 32 343 4922 # # VspI: # [ATTAAT] # 520 522 676 1753 1826 # # XbaI: # [TCTAGA] # 2242 3055 # # XcmI: # [CCANNNNNNNNNTGG] # 175 713 2155 2254 # # XhoII: # [RGATCY] # 24 50 540 1433 3250 # # XmnI: # [GAANNNNTTC] # 315 687 2122 2173 # # ZraI: # [GACGTC] # 1302 3995 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 5297 # Reported_sequences: 1 # Reported_hitcount: 1165 #---------------------------------------