Restriction Fragments for YNL339W-A



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YNL339W-A on chromosome XIV from coordinates 1517 to 2092.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.30727.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 4 8 + TfiI GAWTC 4 7 . . 4 8 + HinfI GANTC 4 7 . . 15 18 + MnlI CCTC 25 24 . . 15 21 + Bpu10I CCTNAGC 16 19 . . 15 21 + BbvCI CCTCAGC 16 19 . . 16 20 + DdeI CTNAG 16 19 . . 16 20 + BspCNI CTCAG 29 27 . . 16 20 + BseMII CTCAG 30 28 . . 21 26 - BsrDI GCAATG 20 18 . . 26 30 + BccI CCATC 34 35 . . 37 42 + AcyI GRCGYC 38 40 . . 42 45 + MnlI CCTC 52 51 . . 38 42 - HgaI GACGC 27 32 . . 45 50 + StyI CCWWGG 45 49 . . 45 50 + SecI* CCNNGG 45 49 . . 53 56 + MaeI CTAG 53 55 . . 62 65 + Csp6I GTAC 62 64 . . 62 65 + RsaI GTAC 63 63 . . 69 75 + TspRI CASTGNN 75 66 . . 89 94 + Eco57MI CTGRAG 110 108 . . 89 94 + GsuI CTGGAG 110 108 . . 100 105 + Cac8I GCNNGC 102 102 . . 100 105 + BmtI GCTAGC 104 100 . . 100 105 + NheI GCTAGC 100 104 . . 101 104 + MaeI CTAG 101 103 . . 104 109 + BtsI GCAGTG 111 109 . . 105 111 + TspRI CASTGNN 111 102 . . 125 130 + Eco57MI CTGRAG 146 144 . . 125 130 + GsuI CTGGAG 146 144 . . 136 141 + Cac8I GCNNGC 138 138 . . 136 141 + BmtI GCTAGC 140 136 . . 136 141 + NheI GCTAGC 136 140 . . 137 140 + MaeI CTAG 137 139 . . 140 145 + BtsI GCAGTG 147 145 . . 141 147 + TspRI CASTGNN 147 138 . . 154 159 + SpeI ACTAGT 154 158 . . 155 158 + MaeI CTAG 155 157 . . 164 168 + PleI GAGTC 172 173 . . 164 168 + MlyI GAGTC 173 173 . . 164 168 + HinfI GANTC 164 167 . . 160 165 - MmeI TCCRAC 141 139 . . 170 173 + Csp6I GTAC 170 172 . . 170 173 + RsaI GTAC 171 171 . . 190 195 + SpeI ACTAGT 190 194 . . 191 194 + MaeI CTAG 191 193 . . 200 204 + PleI GAGTC 208 209 . . 200 204 + MlyI GAGTC 209 209 . . 200 204 + HinfI GANTC 200 203 . . 196 201 - MmeI TCCRAC 177 175 . . 206 209 + Csp6I GTAC 206 208 . . 206 209 + RsaI GTAC 207 207 . . 226 231 + SpeI ACTAGT 226 230 . . 227 230 + MaeI CTAG 227 229 . . 232 237 - MmeI TCCRAC 213 211 . . 242 245 + Csp6I GTAC 242 244 . . 242 245 + RsaI GTAC 243 243 . . 249 255 + TspRI CASTGNN 255 246 . . 262 267 + SpeI ACTAGT 262 266 . . 263 266 + MaeI CTAG 263 265 . . 267 272 + Hpy178III* TCNNGA 268 270 . . 272 275 + TaiI ACGT 275 271 . . 272 275 + SetI ASST 275 271 . . 272 275 + MaeII ACGT 272 274 . . 280 285 + Cac8I GCNNGC 282 282 . . 277 281 - SfaNI GCATC 267 271 . . 284 289 + BtsI GCAGTG 291 289 . . 285 291 + TspRI CASTGNN 291 282 . . 298 303 + SpeI ACTAGT 298 302 . . 299 302 + MaeI CTAG 299 301 . . 303 308 + Hpy178III* TCNNGA 304 306 . . 304 314 + BsiYI* CCNNNNNNNGG 310 307 . . 308 311 + TaiI ACGT 311 307 . . 308 311 + SetI ASST 311 307 . . 308 311 + MaeII ACGT 308 310 . . 316 321 + Cac8I GCNNGC 318 318 . . 320 325 + BtsI GCAGTG 327 325 . . 321 327 + TspRI CASTGNN 327 318 . . 341 346 + Eco57MI CTGRAG 362 360 . . 341 346 + GsuI CTGGAG 362 360 . . 350 353 + Csp6I GTAC 350 352 . . 350 353 + RsaI GTAC 351 351 . . 357 363 + TspRI CASTGNN 363 354 . . 370 375 + SpeI ACTAGT 370 374 . . 371 374 + MaeI CTAG 371 373 . . 375 380 + Hpy178III* TCNNGA 376 378 . . 380 383 + TaiI ACGT 383 379 . . 380 383 + SetI ASST 383 379 . . 380 383 + MaeII ACGT 380 382 . . 388 393 + Cac8I GCNNGC 390 390 . . 385 389 - SfaNI GCATC 375 379 . . 392 397 + BtsI GCAGTG 399 397 . . 393 399 + TspRI CASTGNN 399 390 . . 406 411 + SpeI ACTAGT 406 410 . . 407 410 + MaeI CTAG 407 409 . . 411 416 + Hpy178III* TCNNGA 412 414 . . 412 422 + BsiYI* CCNNNNNNNGG 418 415 . . 416 419 + TaiI ACGT 419 415 . . 416 419 + SetI ASST 419 415 . . 416 419 + MaeII ACGT 416 418 . . 424 429 + Cac8I GCNNGC 426 426 . . 428 433 + BtsI GCAGTG 435 433 . . 429 435 + TspRI CASTGNN 435 426 . . 449 454 + Eco57MI CTGRAG 470 468 . . 449 454 + GsuI CTGGAG 470 468 . . 470 473 + Csp6I GTAC 470 472 . . 470 473 + RsaI GTAC 471 471 . . 472 476 + BsrI ACTGG 477 475 . . 483 492 + MslI CAYNNNNRTG 487 487 . . 489 492 + FatI CATG 488 492 . . 489 492 + NlaIII CATG 492 488 . . 489 492 + CviAII CATG 489 491 . . 490 494 + TspDTI ATGAA 505 503 . . 492 497 + BsmI GAATGC 498 496 . . 495 498 + CviRI* TGCA 496 496 . . 496 506 + MwoI GCNNNNNNNGC 502 499 . . 497 502 + BsaAI YACGTR 499 499 . . 497 502 + PmaCI CACGTG 499 499 . . 498 501 + TaiI ACGT 501 497 . . 498 501 + SetI ASST 501 497 . . 498 501 + MaeII ACGT 498 500 . . 498 503 + AflIII ACRYGT 498 502 . . 510 513 + MnlI CCTC 520 519 . . 516 522 + TspRI CASTGNN 522 513 . . 520 524 + BlsI GCNGC 522 521 . . 520 524 + BisI GCNGC 521 522 . . 520 524 + TseI GCWGC 520 523 . . 516 521 - BtsI GCAGTG 515 513 . . 522 525 + CviRI* TGCA 523 523 . . 518 523 - EcoP15I CAGCAG 490 492 . . 520 524 - BbvI GCAGC 507 511 . . 534 537 + CviRI* TGCA 535 535 . . 537 540 + SetI ASST 540 536 . . 533 538 - BsgI GTGCAG 518 516 . . 541 545 + Tsp45I GTSAC 540 545 . . 541 545 + MaeIII GTNAC 540 545 . . 543 549 + TspRI CASTGNN 549 540 . . 543 548 - BtsI GCAGTG 542 540 . . 557 563 + PfoI TCCNGGA 557 562 . . 558 562 + BssKI CCNGG 557 562 . . 558 562 + BseBI CCWGG 559 560 . . 558 562 + ScrFI CCNGG 559 560 . . 558 562 + EcoRII CCWGG 557 562 . . 564 567 + SetI ASST 567 563 . . 564 567 + CviJI RGCY 565 565 . . 564 567 + AluI AGCT 565 565 . . 566 569 + MaeI CTAG 566 568 . . 570 574 + Tsp4CI* ACNGT 572 571 . . #--------------------------------------- # # # AcyI: # [GRCGYC] # 38 538 # # AflIII: # [ACRYGT] # 78 498 # # AluI: # [AGCT] # 11 565 # # BbvCI: # [CCTCAGC] # 16 560 # # BbvI: # [GCAGC] # 69 507 # # BccI: # [CCATC] # 34 542 # # BisI: # [GCNGC] # 55 521 # # BlsI: # [GCNGC] # 54 522 # # BmtI: # [GCTAGC] # 36 104 436 # # Bpu10I: # [CCTNAGC] # 16 560 # # BsaAI: # [YACGTR] # 77 499 # # BseBI: # [CCWGG] # 17 559 # # BseMII: # [CTCAG] # 30 546 # # BsgI: # [GTGCAG] # 58 518 # # BsiYI*: # [CCNNNNNNNGG] # 108 158 310 # # BsmI: # [GAATGC] # 78 498 # # BspCNI: # [CTCAG] # 29 547 # # BsrDI: # [GCAATG] # 20 556 # # BsrI: # [ACTGG] # 99 477 # # BssKI: # [CCNGG] # 19 557 # # BtsI: # [GCAGTG] # 27 34 36 36 36 72 # 80 111 144 # # Cac8I: # [GCNNGC] # 36 36 36 72 102 144 # 150 # # Csp6I: # [GTAC] # 36 36 62 106 108 108 # 120 # # CviAII: # [CATG] # 87 489 # # CviJI: # [RGCY] # 11 565 # # CviRI*: # [TGCA] # 12 27 41 496 # # DdeI: # [CTNAG] # 16 560 # # Eco57MI: # [CTGRAG] # 36 106 108 110 216 # # EcoP15I: # [CAGCAG] # 86 490 # # EcoRII: # [CCWGG] # 19 557 # # FatI: # [CATG] # 88 488 # # GsuI: # [CTGGAG] # 36 106 108 110 216 # # HgaI: # [GACGC] # 27 549 # # HinfI: # [GANTC] # 4 36 160 376 # # Hpy178III*: # [TCNNGA] # 36 36 72 164 268 # # MaeI: # [CTAG] # 10 18 36 36 36 36 # 36 36 48 53 72 159 # # MaeII: # [ACGT] # 36 36 72 78 82 272 # # MaeIII: # [GTNAC] # 36 540 # # MlyI: # [GAGTC] # 36 173 367 # # MmeI: # [TCCRAC] # 36 36 141 363 # # MnlI: # [CCTC] # 25 27 56 468 # # MslI: # [CAYNNNNRTG] # 89 487 # # MwoI: # [GCNNNNNNNGC] # 74 502 # # NheI: # [GCTAGC] # 36 100 440 # # NlaIII: # [CATG] # 84 492 # # PfoI: # [TCCNGGA] # 19 557 # # PleI: # [GAGTC] # 36 172 368 # # PmaCI: # [CACGTG] # 77 499 # # RsaI: # [GTAC] # 36 36 63 105 108 108 # 120 # # ScrFI: # [CCNGG] # 17 559 # # SecI*: # [CCNNGG] # 45 531 # # SetI: # [ASST] # 9 27 36 36 39 72 # 82 275 # # SfaNI: # [GCATC] # 108 201 267 # # SpeI: # [ACTAGT] # 36 36 36 36 36 72 # 154 170 # # StyI: # [CCWWGG] # 45 531 # # TaiI: # [ACGT] # 36 36 72 75 82 275 # # TfiI: # [GAWTC] # 4 572 # # TseI: # [GCWGC] # 56 520 # # Tsp45I: # [GTSAC] # 36 540 # # Tsp4CI*: # [ACNGT] # 4 572 # # TspDTI: # [ATGAA] # 71 505 # # TspRI: # [CASTGNN] # 27 27 36 36 36 36 # 36 36 36 75 87 108 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 576 # Reported_sequences: 1 # Reported_hitcount: 147 #---------------------------------------