Restriction Fragments for ALP1/YNL270C



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The currently selected gene/sequence is :

ALP1/YNL270C on chromosome XIV from coordinates 137661 to 135940.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.3630.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 7 + FokI GGATG 16 20 . . 3 7 + BseGI GGATG 9 7 . . 5 9 + TspDTI ATGAA 20 18 . . 10 14 + Tsp4CI* ACNGT 12 11 . . 13 18 + Hpy166II GTNNAC 15 15 . . 18 27 + MslI CAYNNNNRTG 22 22 . . 29 34 + StyI CCWWGG 29 33 . . 29 34 + SecI* CCNNGG 29 33 . . 36 39 + SetI ASST 39 35 . . 34 38 - Hin4II* CCTTC 28 27 . . 44 48 + TspDTI ATGAA 59 57 . . 52 55 + TspEI AATT 51 55 . . 56 59 + MaeI CTAG 56 58 . . 58 61 + SetI ASST 61 57 . . 58 61 + CviJI RGCY 59 59 . . 58 61 + AluI AGCT 59 59 . . 73 78 + BseRI GAGGAG 88 86 . . 73 76 - MnlI CCTC 66 65 . . 76 81 + BseRI GAGGAG 91 89 . . 76 79 - MnlI CCTC 69 68 . . 89 93 + TspDTI ATGAA 104 102 . . 95 100 + SspI AATATT 97 97 . . 109 114 + AclI AACGTT 110 112 . . 110 113 + TaiI ACGT 113 109 . . 110 113 + SetI ASST 113 109 . . 110 113 + MaeII ACGT 110 112 . . 112 116 + MaeIII GTNAC 111 116 . . 120 123 + CviJI RGCY 121 121 . . 120 123 + HaeIII GGCC 121 121 . . 117 121 - Hin4II* CCTTC 111 110 . . 122 126 + BccI CCATC 130 131 . . 125 129 - HphI GGTGA 117 116 . . 143 146 + TaqI TCGA 143 145 . . 144 155 + BcgI CGANNNNNNTGC 133 131 167 165 147 151 + FokI GGATG 160 164 . . 147 151 + BseGI GGATG 153 151 . . 145 148 - MnlI CCTC 138 137 . . 154 158 + BlsI GCNGC 156 155 . . 154 158 + BisI GCNGC 155 156 . . 154 158 + TseI GCWGC 154 157 . . 151 155 - SfaNI GCATC 141 145 . . 158 162 + DdeI CTNAG 158 161 . . 154 158 - BbvI GCAGC 141 145 . . 157 163 - Bpu10I CCTNAGC 158 161 . . 171 176 + SacI GAGCTC 175 171 . . 171 176 + Ecl136II GAGCTC 173 173 . . 171 176 + SduI GDGCHC 175 171 . . 171 176 + HgiJII* GRGCYC 175 171 . . 171 176 + HgiAI* GWGCWC 175 171 . . 172 175 + SetI ASST 175 171 . . 172 175 + CviJI RGCY 173 173 . . 172 175 + AluI AGCT 173 173 . . 175 179 - HphI GGTGA 167 166 . . 188 192 + MboII GAAGA 200 199 . . 188 193 - Ksp632I* CTCTTC 183 186 . . 194 197 + SetI ASST 197 193 . . 195 199 + HphI GGTGA 207 206 . . 193 196 - MnlI CCTC 186 185 . . 201 204 - AciI CCGC 201 203 . . 208 211 + MseI TTAA 208 210 . . 213 217 + BlsI GCNGC 215 214 . . 213 217 + BisI GCNGC 214 215 . . 213 217 + BbvI GCAGC 225 229 . . 213 217 + TseI GCWGC 213 216 . . 214 219 + NspBII* CMGCKG 216 216 . . 216 220 + TauI GCSGC 219 216 . . 216 220 + BlsI GCNGC 218 217 . . 216 220 + BisI GCNGC 217 218 . . 216 219 - AciI CCGC 216 218 . . 227 231 + FokI GGATG 240 244 . . 227 231 + BseGI GGATG 233 231 . . 228 238 + Hin4I GAYNNNNNVTC 219 214 251 246 242 246 + TauI GCSGC 245 242 . . 242 246 + BlsI GCNGC 244 243 . . 242 246 + BisI GCNGC 243 244 . . 238 243 - NmeAIII GCCGAG 218 216 . . 244 249 + NlaIV GGNNCC 246 246 . . 244 249 + HgiCI* GGYRCC 244 248 . . 242 245 - AciI CCGC 242 244 . . 253 257 + BslFI GGGAC 267 271 . . 258 262 + BmgT120I GGNCC 259 260 . . 258 262 + AsuI* GGNCC 258 261 . . 259 262 + CviJI RGCY 260 260 . . 259 262 + HaeIII GGCC 260 260 . . 261 264 + MnlI CCTC 271 270 . . 267 270 + TspEI AATT 266 270 . . 277 281 + BmgT120I GGNCC 278 279 . . 277 281 + AvaII GGWCC 277 280 . . 277 281 + AsuI* GGNCC 277 280 . . 280 283 + AciI CCGC 280 282 . . 279 284 - EciI GGCGGA 269 267 . . 295 300 + NaeI GCCGGC 297 297 . . 295 300 + MroNI GCCGGC 295 299 . . 295 300 + Cac8I GCNNGC 297 297 . . 295 300 + Cfr10I RCCGGY 295 299 . . 296 299 + HpaII CCGG 296 298 . . 298 301 + CviJI RGCY 299 299 . . 298 301 + HaeIII GGCC 299 299 . . 298 302 + BmgT120I GGNCC 299 300 . . 298 302 + AsuI* GGNCC 298 301 . . 307 312 + NlaIV GGNNCC 309 309 . . 307 312 + HgiCI* GGYRCC 307 311 . . 315 320 + EcoRV GATATC 317 317 . . 334 338 + BslFI GGGAC 348 352 . . 337 341 + Tsp4CI* ACNGT 339 338 . . 338 344 + TspRI CASTGNN 344 335 . . 352 356 + MaeIII GTNAC 351 356 . . 349 353 - TspGWI ACGGA 339 337 . . 376 380 + Tsp45I GTSAC 375 380 . . 376 380 + MaeIII GTNAC 375 380 . . 372 376 - BccI CCATC 366 367 . . 367 377 - BsaXI ACNNNNNCTCC 389 386 359 356 368 378 - Hin4I GAYNNNNNVTC 391 386 359 354 387 391 + BssKI CCNGG 386 391 . . 387 391 + ScrFI CCNGG 388 389 . . 387 391 + CauII* CCSGG 388 389 . . 388 391 + HpaII CCGG 388 390 . . 391 395 + MaeIII GTNAC 390 395 . . 397 401 - MboII GAAGA 389 388 . . 399 403 - TspDTI ATGAA 389 387 . . 410 414 - MboII GAAGA 402 401 . . 409 414 - BbvII* GAAGAC 402 406 . . 432 437 + Cac8I GCNNGC 434 434 . . 433 443 + BsiYI* CCNNNNNNNGG 439 436 . . 435 440 + HaeII RGCGCY 439 435 . . 435 440 + Eco47III AGCGCT 437 437 . . 436 439 + Hin6I GCGC 436 438 . . 436 439 + GlaI GCGC 437 437 . . 436 446 + MwoI GCNNNNNNNGC 442 439 . . 436 439 + HhaI GCGC 438 436 . . 438 443 - GsaI CCCAGC 442 438 . . 438 443 - BseYI CCCAGC 438 442 . . 452 456 + TspGWI ACGGA 467 465 . . 461 466 + TatI WGTACW 461 465 . . 462 465 + Csp6I GTAC 462 464 . . 462 465 + RsaI GTAC 463 463 . . 464 468 + BsrI ACTGG 469 467 . . 467 470 + CviJI RGCY 468 468 . . 484 487 + SetI ASST 487 483 . . 485 489 + Hin4II* CCTTC 495 494 . . 482 486 - HphI GGTGA 474 473 . . 491 497 + TspRI CASTGNN 497 488 . . 492 496 + BsrI ACTGG 497 495 . . 503 513 + BsiYI* CCNNNNNNNGG 509 506 . . 502 506 - TspGWI ACGGA 492 490 . . 524 529 + TatI WGTACW 524 528 . . 524 529 + ScaI AGTACT 526 526 . . 525 528 + Csp6I GTAC 525 527 . . 525 528 + RsaI GTAC 526 526 . . 527 531 + BsrI ACTGG 532 530 . . 538 543 + BtsI GCAGTG 545 543 . . 539 545 + TspRI CASTGNN 545 536 . . 544 547 + MnlI CCTC 554 553 . . 547 550 + MaeI CTAG 547 549 . . 550 554 + TauI GCSGC 553 550 . . 550 554 + BlsI GCNGC 552 551 . . 550 554 + BisI GCNGC 551 552 . . 552 555 + CviJI RGCY 553 553 . . 550 553 - AciI CCGC 550 552 . . 557 561 + BinI* GGATC 565 566 . . 558 561 + DpnI GATC 559 559 . . 558 561 + BstKTI GATC 560 558 . . 558 567 + BsaBI GATNNNNATC 562 562 . . 558 561 + MboI GATC 557 561 . . 560 565 + Hpy178III* TCNNGA 561 563 . . 564 567 + DpnI GATC 565 565 . . 564 567 + BstKTI GATC 566 564 . . 564 567 + MboI GATC 563 567 . . 572 577 + NlaIV GGNNCC 574 574 . . 572 577 + HgiCI* GGYRCC 572 576 . . 579 584 + Hpy166II GTNNAC 581 581 . . 579 584 + HpaI GTTAAC 581 581 . . 579 584 + HindII GTYRAC 581 581 . . 580 583 + MseI TTAA 580 582 . . 589 593 + TspDTI ATGAA 604 602 . . 593 598 + BspLU11I* ACATGT 593 597 . . 593 598 + NspI RCATGY 597 593 . . 593 598 + AflIII ACRYGT 593 597 . . 594 597 + FatI CATG 593 597 . . 594 597 + NlaIII CATG 597 593 . . 594 597 + CviAII CATG 594 596 . . 614 618 + TspGWI ACGGA 629 627 . . 619 624 + ApoI RAATTY 619 623 . . 620 623 + TspEI AATT 619 623 . . 638 641 + MnlI CCTC 648 647 . . 647 650 + SetI ASST 650 646 . . 654 657 + FnuDII* CGCG 655 655 . . 651 656 - BtgZI GCGATG 636 640 . . 702 706 + BslFI GGGAC 716 720 . . 700 703 - AciI CCGC 700 702 . . 700 704 - FauI CCCGC 693 695 . . 709 713 + Hpy188I TCNGA 711 710 . . 715 720 + NlaIV GGNNCC 717 717 . . 715 719 + BmgT120I GGNCC 716 717 . . 715 719 + AvaII GGWCC 715 718 . . 715 719 + AsuI* GGNCC 715 718 . . 712 716 - BccI CCATC 706 707 . . 719 723 + BccI CCATC 727 728 . . 729 737 + AlwNI CAGNNNCTG 734 731 . . 730 733 + SetI ASST 733 729 . . 732 735 + Csp6I GTAC 732 734 . . 732 735 + RsaI GTAC 733 733 . . 734 738 + BsrI ACTGG 739 737 . . 735 740 + Eco57MI CTGRAG 756 754 . . 735 740 + GsuI CTGGAG 756 754 . . 744 750 + PfoI TCCNGGA 744 749 . . 745 756 + BstXI CCANNNNNNTGG 752 748 . . 745 749 + BssKI CCNGG 744 749 . . 745 749 + BseBI CCWGG 746 747 . . 745 749 + ScrFI CCNGG 746 747 . . 745 749 + EcoRII CCWGG 744 749 . . 748 753 + NlaIV GGNNCC 750 750 . . 750 753 + CviJI RGCY 751 751 . . 752 756 + BssKI CCNGG 751 756 . . 752 756 + BseBI CCWGG 753 754 . . 752 757 + SecI* CCNNGG 752 756 . . 752 756 + ScrFI CCNGG 753 754 . . 752 756 + EcoRII CCWGG 751 756 . . 756 762 + DraII RGGNCCY 757 760 . . 756 761 + NlaIV GGNNCC 758 758 . . 757 761 + BmgT120I GGNCC 758 759 . . 757 761 + AsuI* GGNCC 757 760 . . 758 761 + CviJI RGCY 759 759 . . 758 761 + HaeIII GGCC 759 759 . . 760 764 + BssKI CCNGG 759 764 . . 760 764 + BseBI CCWGG 761 762 . . 760 764 + ScrFI CCNGG 761 762 . . 760 764 + EcoRII CCWGG 759 764 . . 774 780 + TspRI CASTGNN 780 771 . . 773 777 - BsrI ACTGG 773 771 . . 789 793 + BmgT120I GGNCC 790 791 . . 789 793 + AsuI* GGNCC 789 792 . . 787 790 - MnlI CCTC 780 779 . . 790 793 + CviJI RGCY 791 791 . . 790 793 + HaeIII GGCC 791 791 . . 791 795 - BceAI ACGGC 776 778 . . 800 804 + Hpy188I TCNGA 802 801 . . 802 806 + FokI GGATG 815 819 . . 802 806 + BseGI GGATG 808 806 . . 803 807 - BccI CCATC 797 798 . . 812 815 + MnlI CCTC 822 821 . . 820 825 + VspI ATTAAT 821 823 . . 821 824 + MseI TTAA 821 823 . . 826 830 + BlsI GCNGC 828 827 . . 826 830 + BisI GCNGC 827 828 . . 826 830 + TseI GCWGC 826 829 . . 828 831 + CviRI* TGCA 829 829 . . 826 830 - BbvI GCAGC 813 817 . . 834 839 + SnaBI TACGTA 836 836 . . 834 839 + BsaAI YACGTR 836 836 . . 835 838 + TaiI ACGT 838 834 . . 835 838 + SetI ASST 838 834 . . 835 838 + MaeII ACGT 835 837 . . 837 840 + Csp6I GTAC 837 839 . . 837 840 + RsaI GTAC 838 838 . . 840 845 + StyI CCWWGG 840 844 . . 840 845 + SecI* CCNNGG 840 844 . . 843 846 + SetI ASST 846 842 . . 845 848 + Csp6I GTAC 845 847 . . 845 848 + RsaI GTAC 846 846 . . 852 856 + BsrI ACTGG 857 855 . . 866 871 + NspBII* CMGCKG 868 868 . . 866 869 + AciI CCGC 866 868 . . 868 878 + MwoI GCNNNNNNNGC 874 871 . . 871 875 + HphI GGTGA 883 882 . . 877 881 + TauI GCSGC 880 877 . . 877 881 + BlsI GCNGC 879 878 . . 877 881 + BisI GCNGC 878 879 . . 879 882 + CviJI RGCY 880 880 . . 877 880 - AciI CCGC 877 879 . . 894 897 + SetI ASST 897 893 . . 894 897 + CviJI RGCY 895 895 . . 894 897 + AluI AGCT 895 895 . . 899 904 + Hpy178III* TCNNGA 900 902 . . 900 905 + BmeT110I CYCGRG 901 903 . . 900 905 + AvaI CYCGRG 900 904 . . 901 906 + SecI* CCNNGG 901 905 . . 903 906 - MnlI CCTC 896 895 . . 930 934 + TfiI GAWTC 930 933 . . 927 930 - MnlI CCTC 920 919 . . 930 934 + HinfI GANTC 930 933 . . 934 937 + MaeI CTAG 934 936 . . 961 965 - TspDTI ATGAA 951 949 . . 967 970 + CviJI RGCY 968 968 . . 967 970 + HaeIII GGCC 968 968 . . 975 980 + NlaIV GGNNCC 977 977 . . 975 979 + BmgT120I GGNCC 976 977 . . 975 979 + AvaII GGWCC 975 978 . . 975 979 + AsuI* GGNCC 975 978 . . 976 980 - BslFI GGGAC 961 965 . . 990 1000 + BsiYI* CCNNNNNNNGG 996 993 . . 996 1001 - MmeI TCCRAC 977 975 . . 1004 1008 + Tsp45I GTSAC 1003 1008 . . 1004 1008 + MaeIII GTNAC 1003 1008 . . 1007 1011 + Tsp4CI* ACNGT 1009 1008 . . 1018 1022 + BsmAI GTCTC 1023 1027 . . 1020 1025 + Ksp632I* CTCTTC 1026 1029 . . 1021 1025 - MboII GAAGA 1013 1012 . . 1023 1027 - TspDTI ATGAA 1013 1011 . . 1025 1030 - BtgZI GCGATG 1010 1014 . . 1050 1055 + EcoRI GAATTC 1050 1054 . . 1050 1055 + ApoI RAATTY 1050 1054 . . 1051 1054 + TspEI AATT 1050 1054 . . 1082 1085 + MseI TTAA 1082 1084 . . 1086 1089 + FnuDII* CGCG 1087 1087 . . 1088 1093 + McrI* CGRYCG 1091 1089 . . 1087 1090 - AciI CCGC 1087 1089 . . 1100 1104 - HphI GGTGA 1092 1091 . . 1112 1115 + AciI CCGC 1112 1114 . . 1116 1119 + SetI ASST 1119 1115 . . 1118 1122 + MaeIII GTNAC 1117 1122 . . 1114 1119 - BspMI ACCTGC 1105 1109 . . 1129 1134 + Hpy166II GTNNAC 1131 1131 . . 1130 1135 + TatI WGTACW 1130 1134 . . 1130 1135 + Bsp1407I TGTACA 1130 1134 . . 1131 1134 + Csp6I GTAC 1131 1133 . . 1131 1134 + RsaI GTAC 1132 1132 . . 1138 1141 + CviJI RGCY 1139 1139 . . 1140 1145 + SmlI CTYRAG 1140 1144 . . 1141 1146 + Hpy178III* TCNNGA 1142 1144 . . 1140 1145 - Bce83I* CTTGAG 1125 1123 . . 1146 1151 + TatI WGTACW 1146 1150 . . 1146 1151 + ScaI AGTACT 1148 1148 . . 1147 1150 + Csp6I GTAC 1147 1149 . . 1147 1150 + RsaI GTAC 1148 1148 . . 1149 1153 + Tsp4CI* ACNGT 1151 1150 . . 1171 1174 + SetI ASST 1174 1170 . . 1171 1174 + CviJI RGCY 1172 1172 . . 1171 1174 + AluI AGCT 1172 1172 . . 1176 1181 + NarI GGCGCC 1177 1179 . . 1176 1181 + KasI GGCGCC 1176 1180 . . 1176 1181 + DinI GGCGCC 1178 1178 . . 1176 1181 + HaeII RGCGCY 1180 1176 . . 1176 1181 + NlaIV GGNNCC 1178 1178 . . 1176 1181 + AcyI GRCGYC 1177 1179 . . 1176 1181 + HgiCI* GGYRCC 1176 1180 . . 1177 1180 + Hin6I GCGC 1177 1179 . . 1177 1180 + GlaI GCGC 1178 1178 . . 1177 1180 + HhaI GCGC 1179 1177 . . 1180 1185 + StyI CCWWGG 1180 1184 . . 1180 1185 + SecI* CCNNGG 1180 1184 . . 1183 1186 + SetI ASST 1186 1182 . . 1196 1199 + TaiI ACGT 1199 1195 . . 1196 1199 + SetI ASST 1199 1195 . . 1196 1199 + MaeII ACGT 1196 1198 . . 1198 1202 + Tsp45I GTSAC 1197 1202 . . 1198 1202 + MaeIII GTNAC 1197 1202 . . 1206 1209 + SetI ASST 1209 1205 . . 1205 1208 - MnlI CCTC 1198 1197 . . 1205 1216 - PpiI GAACNNNNNCTC 1228 1223 1196 1191 1238 1242 - FokI GGATG 1224 1228 . . 1238 1242 - BseGI GGATG 1237 1235 . . 1240 1244 - TspGWI ACGGA 1230 1228 . . 1247 1251 + Hpy188I TCNGA 1249 1248 . . 1257 1260 + CviJI RGCY 1258 1258 . . 1263 1267 + DdeI CTNAG 1263 1266 . . 1268 1271 + SetI ASST 1271 1267 . . 1267 1270 - MnlI CCTC 1260 1259 . . 1274 1279 + PstI CTGCAG 1278 1274 . . 1274 1279 + SfeI* CTRYAG 1274 1278 . . 1275 1278 + CviRI* TGCA 1276 1276 . . 1276 1281 + Cac8I GCNNGC 1278 1278 . . 1280 1284 + BlsI GCNGC 1282 1281 . . 1280 1284 + BisI GCNGC 1281 1282 . . 1280 1284 + BbvI GCAGC 1292 1296 . . 1280 1284 + TseI GCWGC 1280 1283 . . 1281 1286 + NspBII* CMGCKG 1283 1283 . . 1283 1287 + TauI GCSGC 1286 1283 . . 1283 1287 + BlsI GCNGC 1285 1284 . . 1283 1287 + BisI GCNGC 1284 1285 . . 1283 1286 - AciI CCGC 1283 1285 . . 1289 1294 + StuI AGGCCT 1291 1291 . . 1290 1293 + CviJI RGCY 1291 1291 . . 1290 1293 + HaeIII GGCC 1291 1291 . . 1292 1302 + BsiYI* CCNNNNNNNGG 1298 1295 . . 1295 1298 + MseI TTAA 1295 1297 . . 1298 1302 + BsrI ACTGG 1303 1301 . . 1300 1305 - TsoI TARCCA 1290 1288 . . 1315 1319 + BsrI ACTGG 1320 1318 . . 1322 1327 + CfrI YGGCCR 1322 1326 . . 1323 1326 + CviJI RGCY 1324 1324 . . 1323 1326 + HaeIII GGCC 1324 1324 . . 1324 1329 + Cfr10I RCCGGY 1324 1328 . . 1325 1328 + HpaII CCGG 1325 1327 . . 1337 1341 + BssKI CCNGG 1336 1341 . . 1337 1341 + BseBI CCWGG 1338 1339 . . 1337 1341 + ScrFI CCNGG 1338 1339 . . 1337 1341 + EcoRII CCWGG 1336 1341 . . 1340 1343 + CviJI RGCY 1341 1341 . . 1350 1354 - TspDTI ATGAA 1340 1338 . . 1364 1368 - TspGWI ACGGA 1354 1352 . . 1371 1374 + FatI CATG 1370 1374 . . 1371 1374 + NlaIII CATG 1374 1370 . . 1371 1374 + CviAII CATG 1371 1373 . . 1369 1373 - TspDTI ATGAA 1359 1357 . . 1373 1376 + CviRI* TGCA 1374 1374 . . 1374 1379 + Cac8I GCNNGC 1376 1376 . . 1377 1380 + CviJI RGCY 1378 1378 . . 1389 1392 + TaiI ACGT 1392 1388 . . 1389 1392 + SetI ASST 1392 1388 . . 1389 1392 + MaeII ACGT 1389 1391 . . 1399 1402 + TaqI TCGA 1399 1401 . . 1401 1404 - MnlI CCTC 1394 1393 . . 1404 1408 + FokI GGATG 1417 1421 . . 1404 1408 + BseGI GGATG 1410 1408 . . 1413 1416 + SetI ASST 1416 1412 . . 1416 1421 + PsiI TTATAA 1418 1418 . . 1422 1425 + SetI ASST 1425 1421 . . 1422 1425 + CviJI RGCY 1423 1423 . . 1422 1425 + AluI AGCT 1423 1423 . . 1431 1435 - SfaNI GCATC 1421 1425 . . 1444 1454 + MwoI GCNNNNNNNGC 1450 1447 . . 1444 1454 + BstAPI GCANNNNNTGC 1450 1447 . . 1451 1456 + EcoT22I ATGCAT 1455 1451 . . 1452 1455 + CviRI* TGCA 1453 1453 . . 1453 1457 + SfaNI GCATC 1462 1466 . . 1462 1466 - TspDTI ATGAA 1452 1450 . . 1464 1469 - BtgZI GCGATG 1449 1453 . . 1473 1476 + TspEI AATT 1472 1476 . . 1479 1483 + Hpy188I TCNGA 1481 1480 . . 1482 1485 + DpnI GATC 1483 1483 . . 1482 1485 + BstKTI GATC 1484 1482 . . 1482 1485 + MboI GATC 1481 1485 . . 1485 1489 + BssKI CCNGG 1484 1489 . . 1485 1489 + BseBI CCWGG 1486 1487 . . 1485 1490 + SecI* CCNNGG 1485 1489 . . 1485 1489 + ScrFI CCNGG 1486 1487 . . 1485 1489 + EcoRII CCWGG 1484 1489 . . 1482 1486 - BinI* GGATC 1476 1477 . . 1489 1492 + CviJI RGCY 1490 1490 . . 1496 1499 + AciI CCGC 1496 1498 . . 1493 1497 - HphI GGTGA 1485 1484 . . 1506 1509 + SetI ASST 1509 1505 . . 1517 1520 + CviJI RGCY 1518 1518 . . 1520 1525 + SfeI* CTRYAG 1520 1524 . . 1525 1533 + Tth111I GACNNNGTC 1528 1529 . . 1534 1538 + BlsI GCNGC 1536 1535 . . 1534 1538 + BisI GCNGC 1535 1536 . . 1534 1538 + TseI GCWGC 1534 1537 . . 1536 1539 + CviRI* TGCA 1537 1537 . . 1534 1538 - BbvI GCAGC 1521 1525 . . 1548 1553 + SspI AATATT 1550 1550 . . 1554 1564 + BsiYI* CCNNNNNNNGG 1560 1557 . . 1562 1567 + BalI TGGCCA 1564 1564 . . 1562 1567 + CfrI YGGCCR 1562 1566 . . 1563 1566 + CviJI RGCY 1564 1564 . . 1563 1566 + HaeIII GGCC 1564 1564 . . 1566 1571 + NdeI CATATG 1567 1569 . . 1570 1575 - TsoI TARCCA 1560 1558 . . 1592 1595 + MseI TTAA 1592 1594 . . 1594 1604 + FalI AAGNNNNNCTT 1585 1580 1617 1612 1598 1602 + TauI GCSGC 1601 1598 . . 1598 1602 + BlsI GCNGC 1600 1599 . . 1598 1602 + BisI GCNGC 1599 1600 . . 1599 1602 + AciI CCGC 1599 1601 . . 1594 1604 - FalI AAGNNNNNCTT 1617 1612 1585 1580 1607 1612 + Hpy178III* TCNNGA 1608 1610 . . 1613 1616 + SetI ASST 1616 1612 . . 1613 1616 + CviJI RGCY 1614 1614 . . 1613 1616 + AluI AGCT 1614 1614 . . 1614 1618 + BlsI GCNGC 1616 1615 . . 1614 1618 + BisI GCNGC 1615 1616 . . 1614 1618 + TseI GCWGC 1614 1617 . . 1615 1620 + PstI CTGCAG 1619 1615 . . 1615 1620 + SfeI* CTRYAG 1615 1619 . . 1616 1619 + CviRI* TGCA 1617 1617 . . 1614 1618 - BbvI GCAGC 1601 1605 . . 1621 1626 + EcoRV GATATC 1623 1623 . . 1624 1629 + ClaI ATCGAT 1625 1627 . . 1625 1628 + TaqI TCGA 1625 1627 . . 1627 1632 + EcoRV GATATC 1629 1629 . . 1630 1635 + ClaI ATCGAT 1631 1633 . . 1631 1634 + TaqI TCGA 1631 1633 . . 1633 1637 + TfiI GAWTC 1633 1636 . . 1633 1637 + HinfI GANTC 1633 1636 . . 1636 1640 + Hpy188I TCNGA 1638 1637 . . 1641 1644 + AciI CCGC 1641 1643 . . 1643 1648 + NaeI GCCGGC 1645 1645 . . 1643 1648 + MroNI GCCGGC 1643 1647 . . 1643 1648 + Cac8I GCNNGC 1645 1645 . . 1643 1648 + Cfr10I RCCGGY 1643 1647 . . 1644 1647 + HpaII CCGG 1644 1646 . . 1654 1659 + BseRI GAGGAG 1669 1667 . . 1654 1657 - MnlI CCTC 1647 1646 . . 1673 1676 + CviJI RGCY 1674 1674 . . 1690 1693 + SetI ASST 1693 1689 . . 1707 1711 + BseGI GGATG 1713 1711 . . 1716 1720 - TspDTI ATGAA 1706 1704 . . #--------------------------------------- # # # AciI: # [CCGC] # 11 15 25 26 38 42 # 81 103 150 166 171 201 # 210 213 270 # # AclI: # [AACGTT] # 110 1612 # # AcyI: # [GRCGYC] # 545 1177 # # AflIII: # [ACRYGT] # 593 1129 # # AluI: # [AGCT] # 59 108 114 191 251 277 # 722 # # AlwNI: # [CAGNNNCTG] # 734 988 # # ApoI: # [RAATTY] # 431 619 672 # # AsuI*: # [GGNCC] # 19 21 32 42 186 258 # 417 747 # # AvaI: # [CYCGRG] # 822 900 # # AvaII: # [GGWCC] # 260 277 438 747 # # BalI: # [TGGCCA] # 158 1564 # # BbvI: # [GCAGC] # 80 84 121 141 229 479 # 588 # # BbvII*: # [GAAGAC] # 402 1320 # # BccI: # [CCATC] # 21 70 130 236 340 925 # # Bce83I*: # [CTTGAG] # 597 1125 # # BceAI: # [ACGGC] # 776 946 # # BcgI: # [CGANNNNNNTGC] # 34 133 1555 # # BinI*: # [GGATC] # 246 565 911 # # BisI: # [GCNGC] # 3 3 16 26 51 59 # 64 107 155 251 276 308 # 403 # # BlsI: # [GCNGC] # 3 3 16 26 51 59 # 64 106 156 251 276 308 # 403 # # BmeT110I: # [CYCGRG] # 821 901 # # BmgT120I: # [GGNCC] # 19 21 32 42 186 259 # 417 746 # # Bpu10I: # [CCTNAGC] # 158 1564 # # BsaAI: # [YACGTR] # 836 886 # # BsaBI: # [GATNNNNATC] # 562 1160 # # BsaXI: # [ACNNNNNCTCC] # 30 359 1333 # # BseBI: # [CCWGG] # 7 8 148 236 577 746 # # BseGI: # [GGATG] # 9 9 80 144 173 303 # 429 575 # # BseRI: # [GAGGAG] # 3 53 88 1578 # # BseYI: # [CCCAGC] # 438 1284 # # BsiYI*: # [CCNNNNNNNGG] # 70 162 262 302 439 487 # # BslFI: # [GGGAC] # 81 245 267 368 761 # # BsmAI: # [GTCTC] # 699 1023 # # Bsp1407I: # [TGTACA] # 592 1130 # # BspLU11I*: # [ACATGT] # 593 1129 # # BspMI: # [ACCTGC] # 617 1105 # # BsrI: # [ACTGG] # 17 28 34 35 84 207 # 402 446 469 # # BssKI: # [CCNGG] # 7 8 148 238 358 386 # 577 # # BstAPI: # [GCANNNNNTGC] # 272 1450 # # BstKTI: # [GATC] # 6 238 560 918 # # BstXI: # [CCANNNNNNTGG] # 752 970 # # BtgZI: # [GCGATG] # 273 374 439 636 # # BtsI: # [GCAGTG] # 545 1177 # # Cac8I: # [GCNNGC] # 77 98 137 269 297 844 # # CauII*: # [CCSGG] # 388 1334 # # Cfr10I: # [RCCGGY] # 79 295 319 1029 # # CfrI: # [YGGCCR] # 160 240 1322 # # ClaI: # [ATCGAT] # 6 91 1625 # # Csp6I: # [GTAC] # 8 16 63 105 207 286 # 462 575 # # CviAII: # [CATG] # 351 594 777 # # CviJI: # [RGCY] # 8 15 17 28 32 33 # 33 33 37 39 45 46 # 48 50 52 59 60 62 # 67 73 85 86 87 89 # 169 171 198 # # CviRI*: # [TGCA] # 79 80 84 98 105 447 # 829 # # DdeI: # [CTNAG] # 158 459 1105 # # DinI: # [GGCGCC] # 544 1178 # # DpnI: # [GATC] # 6 239 559 918 # # DraII: # [RGGNCCY] # 757 965 # # EciI: # [GGCGGA] # 269 1453 # # Ecl136II: # [GAGCTC] # 173 1549 # # Eco47III: # [AGCGCT] # 437 1285 # # Eco57MI: # [CTGRAG] # 756 966 # # EcoRI: # [GAATTC] # 672 1050 # # EcoRII: # [CCWGG] # 7 8 148 238 577 744 # # EcoRV: # [GATATC] # 6 93 317 1306 # # EcoT22I: # [ATGCAT] # 267 1455 # # FalI: # [AAGNNNNNCTT] # 0 0 32 105 1585 # # FatI: # [CATG] # 352 593 777 # # FauI: # [CCCGC] # 693 1029 # # FnuDII*: # [CGCG] # 432 635 655 # # FokI: # [GGATG] # 16 80 144 193 305 409 # 575 # # GlaI: # [GCGC] # 437 544 741 # # GsaI: # [CCCAGC] # 442 1280 # # GsuI: # [CTGGAG] # 756 966 # # HaeII: # [RGCGCY] # 439 542 741 # # HaeIII: # [GGCC] # 32 33 39 121 139 158 # 177 240 323 460 # # HgiAI*: # [GWGCWC] # 175 1547 # # HgiCI*: # [GGYRCC] # 63 244 265 546 604 # # HgiJII*: # [GRGCYC] # 175 1547 # # HhaI: # [GCGC] # 438 543 741 # # Hin4I: # [GAYNNNNNVTC] # 32 32 108 219 1331 # # Hin4II*: # [CCTTC] # 28 83 384 1227 # # Hin6I: # [GCGC] # 436 545 741 # # HindII: # [GTYRAC] # 581 1141 # # HinfI: # [GANTC] # 89 703 930 # # HpaI: # [GTTAAC] # 581 1141 # # HpaII: # [CCGG] # 78 92 296 319 937 # # HphI: # [GGTGA] # 40 50 117 209 237 267 # 393 409 # # Hpy166II: # [GTNNAC] # 15 550 566 591 # # Hpy178III*: # [TCNNGA] # 114 242 339 466 561 # # Hpy188I: # [TCNGA] # 84 91 157 232 447 711 # # KasI: # [GGCGCC] # 546 1176 # # Ksp632I*: # [CTCTTC] # 183 696 843 # # MaeI: # [CTAG] # 56 387 491 788 # # MaeII: # [ACGT] # 110 193 333 361 725 # # MaeIII: # [GTNAC] # 15 24 80 111 114 240 # 525 613 # # MboI: # [GATC] # 6 241 557 918 # # MboII: # [GAAGA] # 13 189 200 611 709 # # McrI*: # [CGRYCG] # 631 1091 # # MmeI: # [TCCRAC] # 745 977 # # MnlI: # [CCTC] # 3 24 42 48 62 66 # 69 74 75 85 94 132 # 134 253 278 283 # # MroNI: # [GCCGGC] # 79 295 1348 # # MseI: # [TTAA] # 130 208 213 241 261 297 # 372 # # MslI: # [CAYNNNNRTG] # 22 1700 # # MwoI: # [GCNNNNNNNGC] # 272 432 442 576 # # NaeI: # [GCCGGC] # 77 297 1348 # # NarI: # [GGCGCC] # 545 1177 # # NdeI: # [CATATG] # 155 1567 # # NlaIII: # [CATG] # 348 597 777 # # NlaIV: # [GGNNCC] # 8 33 63 143 201 219 # 246 265 544 # # NmeAIII: # [GCCGAG] # 218 1504 # # NspBII*: # [CMGCKG] # 216 415 439 652 # # NspI: # [RCATGY] # 597 1125 # # PfoI: # [TCCNGGA] # 744 978 # # PpiI: # [GAACNNNNNCTC] # 32 494 1196 # # PsiI: # [TTATAA] # 304 1418 # # PstI: # [CTGCAG] # 103 341 1278 # # RsaI: # [GTAC] # 8 16 63 105 207 286 # 463 574 # # SacI: # [GAGCTC] # 175 1547 # # ScaI: # [AGTACT] # 526 574 622 # # ScrFI: # [CCNGG] # 7 8 148 236 358 388 # 577 # # SduI: # [GDGCHC] # 175 1547 # # SecI*: # [CCNNGG] # 29 61 88 237 279 305 # 723 # # SetI: # [ASST] # 8 9 10 12 13 22 # 22 24 29 39 51 52 # 55 62 62 77 83 84 # 105 107 121 163 222 290 # # SfaNI: # [GCATC] # 41 141 260 1280 # # SfeI*: # [CTRYAG] # 95 107 246 1274 # # SmlI: # [CTYRAG] # 582 1140 # # SnaBI: # [TACGTA] # 836 886 # # SspI: # [AATATT] # 97 172 1453 # # StuI: # [AGGCCT] # 431 1291 # # StyI: # [CCWWGG] # 29 340 542 811 # # TaiI: # [ACGT] # 113 193 330 361 725 # # TaqI: # [TCGA] # 6 91 143 226 1256 # # TatI: # [WGTACW] # 16 63 461 576 606 # # TauI: # [GCSGC] # 26 121 219 308 315 327 # 406 # # TfiI: # [GAWTC] # 89 703 930 # # TseI: # [GCWGC] # 59 80 108 154 254 454 # 613 # # TsoI: # [TARCCA] # 162 270 1290 # # Tsp45I: # [GTSAC] # 194 375 525 628 # # Tsp4CI*: # [ACNGT] # 12 142 327 571 670 # # TspDTI: # [ATGAA] # 16 19 20 39 45 62 # 93 215 254 285 327 347 # # TspEI: # [AATT] # 51 215 250 353 422 431 # # TspGWI: # [ACGGA] # 25 124 128 137 339 368 # 601 # # TspRI: # [CASTGNN] # 48 153 235 344 942 # # Tth111I: # [GACNNNGTC] # 194 1528 # # VspI: # [ATTAAT] # 821 901 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1722 # Reported_sequences: 1 # Reported_hitcount: 488 #---------------------------------------