Restriction Fragments for BSC4/YNL269W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

BSC4/YNL269W on chromosome XIV from coordinates 137699 to 138094.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.27201.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 15 19 + TspGWI ACGGA 30 28 . . 18 22 + MboII GAAGA 30 29 . . 23 27 + MaeIII GTNAC 22 27 . . 18 23 - Ksp632I* CTCTTC 13 16 . . 43 46 + CviRI* TGCA 44 44 . . 44 54 + MwoI GCNNNNNNNGC 50 47 . . 74 79 + PsiI TTATAA 76 76 . . 80 85 + ApoI RAATTY 80 84 . . 81 84 + TspEI AATT 80 84 . . 90 95 + BetI* WCCGGW 90 94 . . 91 94 + HpaII CCGG 91 93 . . 98 102 + Hpy188I TCNGA 100 99 . . 101 106 + SacI GAGCTC 105 101 . . 101 106 + Ecl136II GAGCTC 103 103 . . 101 106 + SduI GDGCHC 105 101 . . 101 106 + HgiJII* GRGCYC 105 101 . . 101 106 + HgiAI* GWGCWC 105 101 . . 102 105 + SetI ASST 105 101 . . 102 105 + CviJI RGCY 103 103 . . 102 105 + AluI AGCT 103 103 . . 103 113 + MwoI GCNNNNNNNGC 109 106 . . 108 113 - BsrDI GCAATG 107 105 . . 124 127 + CviJI RGCY 125 125 . . 126 136 + BsiYI* CCNNNNNNNGG 132 129 . . 142 145 + SetI ASST 145 141 . . 142 145 + CviJI RGCY 143 143 . . 142 145 + AluI AGCT 143 143 . . 143 147 + BlsI GCNGC 145 144 . . 143 147 + BisI GCNGC 144 145 . . 143 147 + TseI GCWGC 143 146 . . 143 147 - BbvI GCAGC 130 134 . . 148 153 + SfeI* CTRYAG 148 152 . . 152 155 + SetI ASST 155 151 . . 161 166 + Hpy178III* TCNNGA 162 164 . . 171 174 + FatI CATG 170 174 . . 171 174 + NlaIII CATG 174 170 . . 171 174 + CviAII CATG 171 173 . . 175 179 + MaeIII GTNAC 174 179 . . 173 178 - TsoI TARCCA 163 161 . . 234 237 + CviJI RGCY 235 235 . . 239 242 + MseI TTAA 239 241 . . 245 249 + TauI GCSGC 248 245 . . 245 249 + BlsI GCNGC 247 246 . . 245 249 + BisI GCNGC 246 247 . . 246 249 + AciI CCGC 246 248 . . 247 250 + FnuDII* CGCG 248 248 . . 250 254 + HinfI GANTC 250 253 . . 250 254 - PleI GAGTC 244 245 . . 250 254 - MlyI GAGTC 244 244 . . 267 271 + Tsp4CI* ACNGT 269 268 . . 272 276 + TauI GCSGC 275 272 . . 272 276 + BlsI GCNGC 274 273 . . 272 276 + BisI GCNGC 273 274 . . 273 276 + AciI CCGC 273 275 . . 277 281 + BceAI ACGGC 293 295 . . 289 294 + HaeII RGCGCY 293 289 . . 290 293 + Hin6I GCGC 290 292 . . 290 293 + GlaI GCGC 291 291 . . 290 293 + HhaI GCGC 292 290 . . 326 329 + MseI TTAA 326 328 . . 335 338 + TspEI AATT 334 338 . . 342 345 + FatI CATG 341 345 . . 342 345 + NlaIII CATG 345 341 . . 342 345 + CviAII CATG 342 344 . . 344 347 + CviRI* TGCA 345 345 . . 349 354 + SecI* CCNNGG 349 353 . . 350 354 + BssKI CCNGG 349 354 . . 350 354 + BseBI CCWGG 351 352 . . 350 354 + ScrFI CCNGG 351 352 . . 350 354 + EcoRII CCWGG 349 354 . . 376 381 + SnaBI TACGTA 378 378 . . 376 381 + BsaAI YACGTR 378 378 . . 377 380 + TaiI ACGT 380 376 . . 377 380 + SetI ASST 380 376 . . 377 380 + MaeII ACGT 377 379 . . 384 387 + Hin6I GCGC 384 386 . . 384 387 + GlaI GCGC 385 385 . . 384 387 + HhaI GCGC 386 384 . . 389 393 - TspDTI ATGAA 379 377 . . #--------------------------------------- # # # AciI: # [CCGC] # 27 123 246 # # AluI: # [AGCT] # 40 103 253 # # ApoI: # [RAATTY] # 80 316 # # BbvI: # [GCAGC] # 130 266 # # BceAI: # [ACGGC] # 103 293 # # BetI*: # [WCCGGW] # 90 306 # # BisI: # [GCNGC] # 27 102 123 144 # # BlsI: # [GCNGC] # 27 102 122 145 # # BsaAI: # [YACGTR] # 18 378 # # BseBI: # [CCWGG] # 45 351 # # BsiYI*: # [CCNNNNNNNGG] # 132 264 # # BsrDI: # [GCAATG] # 107 289 # # BssKI: # [CCNGG] # 47 349 # # CviAII: # [CATG] # 54 171 171 # # CviJI: # [RGCY] # 18 22 92 103 161 # # CviRI*: # [TGCA] # 44 51 301 # # Ecl136II: # [GAGCTC] # 103 293 # # EcoRII: # [CCWGG] # 47 349 # # FatI: # [CATG] # 55 170 171 # # FnuDII*: # [CGCG] # 148 248 # # GlaI: # [GCGC] # 11 94 291 # # HaeII: # [RGCGCY] # 103 293 # # HgiAI*: # [GWGCWC] # 105 291 # # HgiJII*: # [GRGCYC] # 105 291 # # HhaI: # [GCGC] # 10 94 292 # # Hin6I: # [GCGC] # 12 94 290 # # HinfI: # [GANTC] # 146 250 # # HpaII: # [CCGG] # 91 305 # # Hpy178III*: # [TCNNGA] # 162 234 # # Hpy188I: # [TCNGA] # 100 296 # # Ksp632I*: # [CTCTTC] # 13 383 # # MaeII: # [ACGT] # 19 377 # # MaeIII: # [GTNAC] # 22 152 222 # # MboII: # [GAAGA] # 30 366 # # MlyI: # [GAGTC] # 152 244 # # MseI: # [TTAA] # 70 87 239 # # MwoI: # [GCNNNNNNNGC] # 50 59 287 # # NlaIII: # [CATG] # 51 171 174 # # PleI: # [GAGTC] # 152 244 # # PsiI: # [TTATAA] # 76 320 # # SacI: # [GAGCTC] # 105 291 # # ScrFI: # [CCNGG] # 45 351 # # SduI: # [GDGCHC] # 105 291 # # SecI*: # [CCNNGG] # 47 349 # # SetI: # [ASST] # 10 16 40 105 225 # # SfeI*: # [CTRYAG] # 148 248 # # SnaBI: # [TACGTA] # 18 378 # # TaiI: # [ACGT] # 16 380 # # TauI: # [GCSGC] # 27 121 248 # # TseI: # [GCWGC] # 143 253 # # TsoI: # [TARCCA] # 163 233 # # Tsp4CI*: # [ACNGT] # 127 269 # # TspDTI: # [ATGAA] # 17 379 # # TspEI: # [AATT] # 62 80 254 # # TspGWI: # [ACGGA] # 30 366 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 396 # Reported_sequences: 1 # Reported_hitcount: 79 #---------------------------------------