Restriction Fragments for GIS2/YNL255C



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The currently selected gene/sequence is :

GIS2/YNL255C on chromosome XIV from coordinates 167790 to 167329.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.28935.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 7 + BsmAI GTCTC 8 12 . . 11 16 + HindIII AAGCTT 11 15 . . 12 15 + SetI ASST 15 11 . . 12 15 + CviJI RGCY 13 13 . . 12 15 + AluI AGCT 13 13 . . 17 21 + MaeIII GTNAC 16 21 . . 20 23 + TaiI ACGT 23 19 . . 20 23 + SetI ASST 23 19 . . 20 23 + MaeII ACGT 20 22 . . 26 29 - AciI CCGC 26 28 . . 36 41 + BalI TGGCCA 38 38 . . 36 41 + CfrI YGGCCR 36 40 . . 37 40 + CviJI RGCY 38 38 . . 37 40 + HaeIII GGCC 38 38 . . 49 53 + MboII GAAGA 61 60 . . 49 54 + BbvII* GAAGAC 56 60 . . 53 57 + Tsp4CI* ACNGT 55 54 . . 58 62 + TfiI GAWTC 58 61 . . 58 62 + HinfI GANTC 58 61 . . 61 65 + Hpy188I TCNGA 63 62 . . 74 78 + MaeIII GTNAC 73 78 . . 80 84 + Tsp4CI* ACNGT 82 81 . . 83 87 + MaeIII GTNAC 82 87 . . 91 95 + BssKI CCNGG 90 95 . . 91 95 + ScrFI CCNGG 92 93 . . 91 95 + CauII* CCSGG 92 93 . . 92 95 + HpaII CCGG 92 94 . . 95 99 + Tsp45I GTSAC 94 99 . . 95 99 + MaeIII GTNAC 94 99 . . 98 101 + TaiI ACGT 101 97 . . 98 101 + SetI ASST 101 97 . . 98 101 + MaeII ACGT 98 100 . . 106 110 + TspGWI ACGGA 121 119 . . 113 116 + Csp6I GTAC 113 115 . . 113 116 + RsaI GTAC 114 114 . . 117 120 + FatI CATG 116 120 . . 117 120 + NlaIII CATG 120 116 . . 117 120 + CviAII CATG 117 119 . . 127 131 + Tsp4CI* ACNGT 129 128 . . 133 138 + EcoRI GAATTC 133 137 . . 133 138 + ApoI RAATTY 133 137 . . 134 137 + TspEI AATT 133 137 . . 141 146 + BsrDI GCAATG 148 146 . . 146 150 + MaeIII GTNAC 145 150 . . 150 155 + MfeI CAATTG 150 154 . . 151 154 + TspEI AATT 150 154 . . 157 161 + HphI GGTGA 169 168 . . 159 170 + BdaI TGANNNNNNTCA 148 146 182 180 163 168 + Cfr10I RCCGGY 163 167 . . 163 168 + BetI* WCCGGW 163 167 . . 163 168 + AgeI ACCGGT 163 167 . . 164 167 + HpaII CCGG 164 166 . . 167 171 + Tsp45I GTSAC 166 171 . . 167 171 + MaeIII GTNAC 166 171 . . 170 173 + TaiI ACGT 173 169 . . 170 173 + SetI ASST 173 169 . . 170 173 + MaeII ACGT 170 172 . . 159 170 - BdaI TGANNNNNNTCA 182 180 148 146 176 188 + BarI GAAGNNNNNNTAC 168 163 200 195 184 189 + TatI WGTACW 184 188 . . 185 188 + Csp6I GTAC 185 187 . . 185 188 + RsaI GTAC 186 186 . . 187 191 + Tsp4CI* ACNGT 189 188 . . 191 194 + CviRI* TGCA 192 192 . . 194 199 + AclI AACGTT 195 197 . . 195 198 + TaiI ACGT 198 194 . . 195 198 + SetI ASST 198 194 . . 195 198 + MaeII ACGT 195 197 . . 206 210 + Tsp4CI* ACNGT 208 207 . . 209 213 + MaeIII GTNAC 208 213 . . 210 215 + TsoI TARCCA 226 224 . . 217 222 + Cfr10I RCCGGY 217 221 . . 218 221 + HpaII CCGG 218 220 . . 219 224 + CfrI YGGCCR 219 223 . . 220 223 + CviJI RGCY 221 221 . . 220 223 + HaeIII GGCC 221 221 . . 229 234 + Hpy178III* TCNNGA 230 232 . . 228 233 - Eco57MI CTGRAG 213 211 . . 228 233 - GsuI CTGGAG 213 211 . . 235 240 + BsmI GAATGC 241 239 . . 252 256 + MboII GAAGA 264 263 . . 252 257 + BbvII* GAAGAC 259 263 . . 258 263 + EcoP15I CAGCAG 288 290 . . 263 267 + TfiI GAWTC 263 266 . . 263 267 + HinfI GANTC 263 266 . . 278 281 + FatI CATG 277 281 . . 278 281 + NlaIII CATG 281 277 . . 278 281 + CviAII CATG 278 280 . . 276 280 - TspDTI ATGAA 266 264 . . 282 287 + PsiI TTATAA 284 284 . . 295 299 + BmgT120I GGNCC 296 297 . . 295 299 + AvaII GGWCC 295 298 . . 295 299 + AsuI* GGNCC 295 298 . . 304 309 + NdeI CATATG 305 307 . . 309 312 + CviJI RGCY 310 310 . . 311 315 + DdeI CTNAG 311 314 . . 308 313 - TsoI TARCCA 298 296 . . 310 316 - Bpu10I CCTNAGC 311 314 . . 317 321 + Tsp4CI* ACNGT 319 318 . . 322 326 + TspDTI ATGAA 337 335 . . 328 331 - MnlI CCTC 321 320 . . 332 336 + TspGWI ACGGA 347 345 . . 336 339 + TspEI AATT 335 339 . . 353 357 + MaeIII GTNAC 352 357 . . 369 372 + SetI ASST 372 368 . . 369 372 + CviJI RGCY 370 370 . . 369 372 + AluI AGCT 370 370 . . 370 375 + Cac8I GCNNGC 372 372 . . 372 377 + BalI TGGCCA 374 374 . . 372 377 + CfrI YGGCCR 372 376 . . 373 376 + CviJI RGCY 374 374 . . 373 376 + HaeIII GGCC 374 374 . . 377 382 + BspLU11I* ACATGT 377 381 . . 377 382 + NspI RCATGY 381 377 . . 377 382 + AflIII ACRYGT 377 381 . . 378 381 + FatI CATG 377 381 . . 378 381 + NlaIII CATG 381 377 . . 378 381 + CviAII CATG 378 380 . . 383 387 + BssKI CCNGG 382 387 . . 383 387 + BseBI CCWGG 384 385 . . 383 388 + SecI* CCNNGG 383 387 . . 383 387 + ScrFI CCNGG 384 385 . . 383 387 + EcoRII CCWGG 382 387 . . 384 392 + AlwNI CAGNNNCTG 389 386 . . 386 390 + BslFI GGGAC 400 404 . . 389 393 + Tsp4CI* ACNGT 391 390 . . 404 407 + CviJI RGCY 405 405 . . 410 414 + MaeIII GTNAC 409 414 . . 416 420 + Tsp4CI* ACNGT 418 417 . . 419 423 + MaeIII GTNAC 418 423 . . 427 431 + BsrI ACTGG 432 430 . . 429 434 + BalI TGGCCA 431 431 . . 429 434 + CfrI YGGCCR 429 433 . . 430 433 + CviJI RGCY 431 431 . . 430 433 + HaeIII GGCC 431 431 . . 440 445 + StyI CCWWGG 440 444 . . 440 445 + SecI* CCNNGG 440 444 . . 456 459 + CviJI RGCY 457 457 . . 458 462 + DdeI CTNAG 458 461 . . #--------------------------------------- # # # AciI: # [CCGC] # 26 436 # # AclI: # [AACGTT] # 195 267 # # AflIII: # [ACRYGT] # 85 377 # # AgeI: # [ACCGGT] # 163 299 # # AluI: # [AGCT] # 13 92 357 # # AlwNI: # [CAGNNNCTG] # 73 389 # # ApoI: # [RAATTY] # 133 329 # # AsuI*: # [GGNCC] # 167 295 # # AvaII: # [GGWCC] # 167 295 # # BalI: # [TGGCCA] # 31 38 57 336 # # BarI: # [GAAGNNNNNNTAC] # 32 168 262 # # BbvII*: # [GAAGAC] # 56 203 203 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 148 280 # # BetI*: # [WCCGGW] # 163 299 # # BmgT120I: # [GGNCC] # 166 296 # # Bpu10I: # [CCTNAGC] # 151 311 # # BseBI: # [CCWGG] # 78 384 # # BslFI: # [GGGAC] # 62 400 # # BsmAI: # [GTCTC] # 8 454 # # BsmI: # [GAATGC] # 221 241 # # BspLU11I*: # [ACATGT] # 85 377 # # BsrDI: # [GCAATG] # 148 314 # # BsrI: # [ACTGG] # 30 432 # # BssKI: # [CCNGG] # 80 90 292 # # Cac8I: # [GCNNGC] # 90 372 # # CauII*: # [CCSGG] # 92 370 # # Cfr10I: # [RCCGGY] # 54 163 245 # # CfrI: # [YGGCCR] # 33 36 57 153 183 # # Csp6I: # [GTAC] # 72 113 277 # # CviAII: # [CATG] # 84 100 117 161 # # CviJI: # [RGCY] # 4 5 13 25 26 26 # 31 60 89 183 # # CviRI*: # [TGCA] # 192 270 # # DdeI: # [CTNAG] # 4 147 311 # # Eco57MI: # [CTGRAG] # 213 249 # # EcoP15I: # [CAGCAG] # 174 288 # # EcoRI: # [GAATTC] # 133 329 # # EcoRII: # [CCWGG] # 80 382 # # FatI: # [CATG] # 85 100 116 161 # # GsuI: # [CTGGAG] # 213 249 # # HaeIII: # [GGCC] # 31 38 57 153 183 # # HindIII: # [AAGCTT] # 11 451 # # HinfI: # [GANTC] # 58 199 205 # # HpaII: # [CCGG] # 54 72 92 244 # # HphI: # [GGTGA] # 169 293 # # Hpy178III*: # [TCNNGA] # 230 232 # # Hpy188I: # [TCNGA] # 63 399 # # MaeII: # [ACGT] # 20 25 72 78 267 # # MaeIII: # [GTNAC] # 9 9 12 16 21 42 # 44 51 57 57 144 # # MboII: # [GAAGA] # 61 198 203 # # MfeI: # [CAATTG] # 150 312 # # MnlI: # [CCTC] # 141 321 # # NdeI: # [CATATG] # 157 305 # # NlaIII: # [CATG] # 81 100 120 161 # # NspI: # [RCATGY] # 81 381 # # PsiI: # [TTATAA] # 178 284 # # RsaI: # [GTAC] # 72 114 276 # # ScrFI: # [CCNGG] # 78 92 292 # # SecI*: # [CCNNGG] # 22 57 383 # # SetI: # [ASST] # 8 15 25 72 78 90 # 174 # # StyI: # [CCWWGG] # 22 440 # # TaiI: # [ACGT] # 23 25 72 78 264 # # TatI: # [WGTACW] # 184 278 # # TfiI: # [GAWTC] # 58 199 205 # # TsoI: # [TARCCA] # 72 164 226 # # Tsp45I: # [GTSAC] # 72 94 296 # # Tsp4CI*: # [ACNGT] # 19 27 27 44 47 55 # 60 72 111 # # TspDTI: # [ATGAA] # 71 125 266 # # TspEI: # [AATT] # 17 127 133 185 # # TspGWI: # [ACGGA] # 115 121 226 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 462 # Reported_sequences: 1 # Reported_hitcount: 139 #---------------------------------------